BR112014029252B8 - Método para a construção de uma célula hospedeira fúngica recombinante, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesse - Google Patents
Método para a construção de uma célula hospedeira fúngica recombinante, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesseInfo
- Publication number
- BR112014029252B8 BR112014029252B8 BR112014029252A BR112014029252A BR112014029252B8 BR 112014029252 B8 BR112014029252 B8 BR 112014029252B8 BR 112014029252 A BR112014029252 A BR 112014029252A BR 112014029252 A BR112014029252 A BR 112014029252A BR 112014029252 B8 BR112014029252 B8 BR 112014029252B8
- Authority
- BR
- Brazil
- Prior art keywords
- fungal host
- host cell
- recombinant fungal
- polynucleotide
- polypeptide
- Prior art date
Links
- 230000002538 fungal effect Effects 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 6
- 229920001184 polypeptide Polymers 0.000 title abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
- 238000010276 construction Methods 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 abstract 7
- 239000002157 polynucleotide Substances 0.000 abstract 7
- 102000040430 polynucleotide Human genes 0.000 abstract 7
- 108091026898 Leader sequence (mRNA) Proteins 0.000 abstract 1
- 108020004422 Riboswitch Proteins 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
- C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
- C12Y107/01003—Nitrate reductase (NADPH) (1.7.1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
- C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
- C12Y107/01004—Nitrite reductase [NAD(P)H] (1.7.1.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01011—Phosphopyruvate hydratase (4.2.1.11), i.e. enolase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12170260 | 2012-05-31 | ||
| EP12170260.9 | 2012-05-31 | ||
| PCT/EP2013/061052 WO2013178674A1 (en) | 2012-05-31 | 2013-05-29 | Improved selection in fungi |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112014029252A2 BR112014029252A2 (pt) | 2017-07-18 |
| BR112014029252B1 BR112014029252B1 (pt) | 2021-10-19 |
| BR112014029252B8 true BR112014029252B8 (pt) | 2022-01-25 |
Family
ID=48539153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112014029252A BR112014029252B8 (pt) | 2012-05-31 | 2013-05-29 | Método para a construção de uma célula hospedeira fúngica recombinante, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesse |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US9487767B2 (enExample) |
| EP (2) | EP2855659A1 (enExample) |
| JP (1) | JP6449763B2 (enExample) |
| CN (1) | CN104395454B (enExample) |
| BR (1) | BR112014029252B8 (enExample) |
| DK (1) | DK3327113T3 (enExample) |
| WO (1) | WO2013178674A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170027167A1 (en) | 2014-03-05 | 2017-02-02 | Novozymes Bioag A/S | Formulations comprising polymeric xyloglucan as a carrier for agriculturally beneficial agents |
| EP3113609B1 (en) | 2014-03-05 | 2019-07-31 | Novozymes A/S | Methods for improving post-harvest properties of agricultural crops |
| WO2015134737A1 (en) | 2014-03-05 | 2015-09-11 | Novozymes A/S | Compositions and methods for improving properties of cellulosic textile materials with xyloglucan endotransglycosylase |
| CN106062276B (zh) | 2014-03-05 | 2019-06-11 | 诺维信公司 | 用于将材料功能化并且进行连接的组合物和方法 |
| US20160348035A1 (en) | 2014-03-05 | 2016-12-01 | Novozymes A/S | Compositions and Methods for Improving Properties of Non-Cellulosic Textile Materials with Xyloglucan Endotransglycosylase |
| CN106795504A (zh) | 2014-08-20 | 2017-05-31 | 诺维信公司 | 木葡聚糖内糖基转移酶变体以及编码其的多核苷酸 |
| US10781428B2 (en) | 2014-12-02 | 2020-09-22 | Novozymes A/S | Laccase variants and polynucleotides encoding same |
| EA202092665A3 (ru) | 2015-02-02 | 2021-06-30 | МЕИРЭДжТиЭкс ЮКей II ЛИМИТЕД | Регулирование экспрессии генов посредством аптамеропосредованного модулирования альтернативного сплайсинга |
| WO2016160584A1 (en) * | 2015-03-27 | 2016-10-06 | Cargill, Incorporated | Glucoamylase-modified yeast strains and methods for bioproduct production |
| WO2016174258A1 (en) | 2015-04-29 | 2016-11-03 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Means and methods for protein production |
| CN108431229A (zh) | 2015-12-17 | 2018-08-21 | 嘉吉公司 | 糖转运蛋白修饰的酵母菌株和用于生物制品生产的方法 |
| MX2019001239A (es) | 2016-08-03 | 2019-07-04 | Meiragtx Uk Ii Ltd | Deteccion de alto rendimiento basada en celulas para aptámeros. |
| US11421212B2 (en) | 2016-08-05 | 2022-08-23 | Cargill, Incorporated | Engineered yeast strains with signal sequence-modified glucoamylase polypeptides and enhanced ethanol production |
| CN108949579B (zh) * | 2018-04-19 | 2021-10-01 | 中国科学技术大学 | 嗜热子囊菌基因表达系统 |
| US12398411B2 (en) | 2018-06-15 | 2025-08-26 | Kikkoman Corporation | Method for producing selenoneine |
| WO2020025357A1 (en) | 2018-08-02 | 2020-02-06 | Novozymes A/S | Preparation of combinatorial libraries of dna constructs using introns |
| EP3898985B1 (en) | 2018-12-21 | 2023-02-08 | Novozymes A/S | Tandem protein expression |
| CN110592149B (zh) * | 2019-07-16 | 2020-07-14 | 甘肃省科学院生物研究所 | 一种梨囊鞭菌发酵秸秆生产乳酸的新方法和应用 |
| CN114391038A (zh) | 2019-07-25 | 2022-04-22 | 诺维信公司 | 丝状真菌表达系统 |
| US11479779B2 (en) * | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
| CN114292761B (zh) * | 2021-12-02 | 2023-11-03 | 南京工业大学 | 一株黑曲霉基因工程菌及构建方法与应用 |
| WO2025133003A2 (en) | 2023-12-19 | 2025-06-26 | 21St.Bio A/S | Non-animal bovine beta lactoglobulin |
| WO2025140482A1 (zh) * | 2023-12-28 | 2025-07-03 | 南京百斯杰生物工程有限公司 | 可改善目标多肽表达效果的多核苷酸构建体及其应用 |
| WO2025226596A1 (en) | 2024-04-24 | 2025-10-30 | Novozymes A/S | Methods for producing secreted polypeptides |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| AU694954B2 (en) | 1994-06-03 | 1998-08-06 | Novo Nordisk A/S | Purified myceliophthora laccases and nucleic acids encoding same |
| EP1559776A3 (en) | 1994-06-30 | 2006-01-11 | Novozymes Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system and promoters and terminators for use therein |
| WO1998011203A1 (en) | 1996-09-13 | 1998-03-19 | Novo Nordisk Biotech, Inc. | Cells having dna insertion mutations which produce altered amounts of a polypeptide |
| CN1197965C (zh) | 1998-10-26 | 2005-04-20 | 诺维信公司 | 在丝状真菌细胞内构建和筛选目的dna文库 |
| CN1526820B (zh) | 1999-03-22 | 2010-05-26 | 诺沃奇梅兹有限公司 | 用于在真菌细胞中表达基因的启动子 |
| AU2001262063A1 (en) | 2000-05-24 | 2001-12-03 | Novozymes A/S | Method for increasing gene copy number in a host cell and resulting host cell |
| JP2007259787A (ja) * | 2006-03-29 | 2007-10-11 | Hakutsuru Shuzo Kk | 遺伝子発現制御ポリヌクレオチド |
| GB201107768D0 (en) | 2011-05-10 | 2011-06-22 | Univ Manchester | Riboswitches |
-
2013
- 2013-05-29 BR BR112014029252A patent/BR112014029252B8/pt active Search and Examination
- 2013-05-29 EP EP13726188.9A patent/EP2855659A1/en not_active Withdrawn
- 2013-05-29 EP EP17200169.5A patent/EP3327113B1/en active Active
- 2013-05-29 CN CN201380028411.5A patent/CN104395454B/zh active Active
- 2013-05-29 DK DK17200169.5T patent/DK3327113T3/da active
- 2013-05-29 US US14/403,355 patent/US9487767B2/en active Active
- 2013-05-29 JP JP2015514483A patent/JP6449763B2/ja active Active
- 2013-05-29 WO PCT/EP2013/061052 patent/WO2013178674A1/en not_active Ceased
-
2016
- 2016-10-18 US US15/296,433 patent/US10351861B2/en active Active
-
2018
- 2018-06-26 US US16/018,726 patent/US10351863B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| BR112014029252A2 (pt) | 2017-07-18 |
| JP6449763B2 (ja) | 2019-01-09 |
| CN104395454A (zh) | 2015-03-04 |
| CN104395454B (zh) | 2021-11-30 |
| US20150175981A1 (en) | 2015-06-25 |
| US9487767B2 (en) | 2016-11-08 |
| JP2015517816A (ja) | 2015-06-25 |
| US10351861B2 (en) | 2019-07-16 |
| WO2013178674A1 (en) | 2013-12-05 |
| EP2855659A1 (en) | 2015-04-08 |
| EP3327113A2 (en) | 2018-05-30 |
| DK3327113T3 (da) | 2021-09-13 |
| US10351863B2 (en) | 2019-07-16 |
| US20180298394A1 (en) | 2018-10-18 |
| BR112014029252B1 (pt) | 2021-10-19 |
| EP3327113B1 (en) | 2021-08-18 |
| US20170029830A1 (en) | 2017-02-02 |
| EP3327113A3 (en) | 2018-08-08 |
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Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA. |
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