JP6449763B2 - 真菌における改善された選択 - Google Patents

真菌における改善された選択 Download PDF

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JP6449763B2
JP6449763B2 JP2015514483A JP2015514483A JP6449763B2 JP 6449763 B2 JP6449763 B2 JP 6449763B2 JP 2015514483 A JP2015514483 A JP 2015514483A JP 2015514483 A JP2015514483 A JP 2015514483A JP 6449763 B2 JP6449763 B2 JP 6449763B2
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seq
polynucleotide
host cell
intron
fungal host
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JP2015517816A5 (enExample
JP2015517816A (ja
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リレルンド オルセン カーステン
リレルンド オルセン カーステン
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ノボザイムス アクティーゼルスカブ
ノボザイムス アクティーゼルスカブ
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01003—Nitrate reductase (NADPH) (1.7.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01004—Nitrite reductase [NAD(P)H] (1.7.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01011—Phosphopyruvate hydratase (4.2.1.11), i.e. enolase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2015514483A 2012-05-31 2013-05-29 真菌における改善された選択 Active JP6449763B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12170260.9 2012-05-31
EP12170260 2012-05-31
PCT/EP2013/061052 WO2013178674A1 (en) 2012-05-31 2013-05-29 Improved selection in fungi

Publications (3)

Publication Number Publication Date
JP2015517816A JP2015517816A (ja) 2015-06-25
JP2015517816A5 JP2015517816A5 (enExample) 2016-07-21
JP6449763B2 true JP6449763B2 (ja) 2019-01-09

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JP2015514483A Active JP6449763B2 (ja) 2012-05-31 2013-05-29 真菌における改善された選択

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US (3) US9487767B2 (enExample)
EP (2) EP2855659A1 (enExample)
JP (1) JP6449763B2 (enExample)
CN (1) CN104395454B (enExample)
BR (1) BR112014029252B8 (enExample)
DK (1) DK3327113T3 (enExample)
WO (1) WO2013178674A1 (enExample)

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CN106062271A (zh) 2014-03-05 2016-10-26 诺维信公司 用于改进具有木葡聚糖内糖基转移酶的纤维素纺织材料的性质的组合物和方法
CN106062270A (zh) 2014-03-05 2016-10-26 诺维信公司 使用木葡聚糖内糖基转移酶改进非纤维素纺织材料的性质的组合物和方法
EP3113609B1 (en) 2014-03-05 2019-07-31 Novozymes A/S Methods for improving post-harvest properties of agricultural crops
EP3114276B1 (en) 2014-03-05 2019-01-16 Novozymes A/S Compositions and methods for functionalizing and linking materials
EP3113611A1 (en) 2014-03-05 2017-01-11 Novozymes A/S Formulations comprising polymeric xyloglucan as a carrier for agriculturally beneficial agents
US20170267980A1 (en) 2014-08-20 2017-09-21 Novozymes A/S Xyloglucan Endotransglycosylase variants and Polynucleotides Encoding Same
WO2016090059A1 (en) 2014-12-02 2016-06-09 Novozymes A/S Laccase variants and polynucleotides encoding same
WO2016126747A1 (en) 2015-02-02 2016-08-11 Meiragtx Uk Limited Regulation of gene expression by aptamer-mediated modulation of alternative splicing
EP3274461A1 (en) * 2015-03-27 2018-01-31 Cargill, Incorporated Glucoamylase-modified yeast strains and methods for bioproduct production
WO2016174258A1 (en) 2015-04-29 2016-11-03 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for protein production
BR112018011902A2 (pt) 2015-12-17 2018-12-04 Cargill Inc método de fermentação, levedura geneticamente modificada, construto de ácido nucleico, vetor, célula hospedeira, meio de fermentação e utilização da levedura geneticamente modificada
EP3494229B1 (en) * 2016-08-03 2023-10-25 Meiragtx UK II Limited High throughput cell-based screening for aptamers
WO2018027131A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
CN108949579B (zh) * 2018-04-19 2021-10-01 中国科学技术大学 嗜热子囊菌基因表达系统
EP3808853A4 (en) 2018-06-15 2022-05-18 Kikkoman Corporation Selenoneine production method
WO2020025357A1 (en) 2018-08-02 2020-02-06 Novozymes A/S Preparation of combinatorial libraries of dna constructs using introns
WO2020127198A1 (en) 2018-12-21 2020-06-25 Novozymes A/S Tandem protein expression
CN110592149B (zh) * 2019-07-16 2020-07-14 甘肃省科学院生物研究所 一种梨囊鞭菌发酵秸秆生产乳酸的新方法和应用
EP4004211A1 (en) 2019-07-25 2022-06-01 Novozymes A/S Filamentous fungal expression system
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CN114292761B (zh) * 2021-12-02 2023-11-03 南京工业大学 一株黑曲霉基因工程菌及构建方法与应用
WO2025133003A2 (en) 2023-12-19 2025-06-26 21St.Bio A/S Non-animal bovine beta lactoglobulin
WO2025140482A1 (zh) * 2023-12-28 2025-07-03 南京百斯杰生物工程有限公司 可改善目标多肽表达效果的多核苷酸构建体及其应用
WO2025226596A1 (en) 2024-04-24 2025-10-30 Novozymes A/S Methods for producing secreted polypeptides

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DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
CA2191718A1 (en) 1994-06-03 1995-12-14 Randy M. Berka Phosphonyldipeptides useful in the treatment of cardiovascular diseases
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
JP4436934B2 (ja) 1996-09-13 2010-03-24 ノボザイムス，インコーポレイティド 変更された量のポリペプチドを生成するｄｎａ挿入突然変異を有する細胞
JP5043254B2 (ja) 1998-10-26 2012-10-10 ノボザイムス アクティーゼルスカブ 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング
EP1194572A2 (en) 1999-03-22 2002-04-10 Novozymes Biotech, Inc. Promoter sequences derived from fusarium venenatum and uses thereof
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JP2007259787A (ja) * 2006-03-29 2007-10-11 Hakutsuru Shuzo Kk 遺伝子発現制御ポリヌクレオチド
GB201107768D0 (en) 2011-05-10 2011-06-22 Univ Manchester Riboswitches

Also Published As

Publication number Publication date
CN104395454B (zh) 2021-11-30
BR112014029252A2 (pt) 2017-07-18
BR112014029252B1 (pt) 2021-10-19
WO2013178674A1 (en) 2013-12-05
US20150175981A1 (en) 2015-06-25
EP3327113A3 (en) 2018-08-08
US10351863B2 (en) 2019-07-16
BR112014029252B8 (pt) 2022-01-25
CN104395454A (zh) 2015-03-04
DK3327113T3 (da) 2021-09-13
US20170029830A1 (en) 2017-02-02
US20180298394A1 (en) 2018-10-18
US10351861B2 (en) 2019-07-16
JP2015517816A (ja) 2015-06-25
EP3327113B1 (en) 2021-08-18
US9487767B2 (en) 2016-11-08
EP2855659A1 (en) 2015-04-08
EP3327113A2 (en) 2018-05-30

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