CN104395454B - 改善的真菌选择 - Google Patents

改善的真菌选择 Download PDF

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CN104395454B
CN104395454B CN201380028411.5A CN201380028411A CN104395454B CN 104395454 B CN104395454 B CN 104395454B CN 201380028411 A CN201380028411 A CN 201380028411A CN 104395454 B CN104395454 B CN 104395454B
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host cell
fungal host
polynucleotide construct
polynucleotide
site
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CN104395454A (zh
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C·L·奥尔森
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Novozymes AS
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Novozymes AS
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
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    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01003—Nitrate reductase (NADPH) (1.7.1.3)
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    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01004—Nitrite reductase [NAD(P)H] (1.7.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01011—Phosphopyruvate hydratase (4.2.1.11), i.e. enolase

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  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Molecular Biology (AREA)
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  • Mycology (AREA)
  • Physics & Mathematics (AREA)
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  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201380028411.5A 2012-05-31 2013-05-29 改善的真菌选择 Active CN104395454B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12170260 2012-05-31
EP12170260.9 2012-05-31
PCT/EP2013/061052 WO2013178674A1 (en) 2012-05-31 2013-05-29 Improved selection in fungi

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CN104395454A CN104395454A (zh) 2015-03-04
CN104395454B true CN104395454B (zh) 2021-11-30

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US (3) US9487767B2 (enExample)
EP (2) EP2855659A1 (enExample)
JP (1) JP6449763B2 (enExample)
CN (1) CN104395454B (enExample)
BR (1) BR112014029252B8 (enExample)
DK (1) DK3327113T3 (enExample)
WO (1) WO2013178674A1 (enExample)

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US20170027167A1 (en) 2014-03-05 2017-02-02 Novozymes Bioag A/S Formulations comprising polymeric xyloglucan as a carrier for agriculturally beneficial agents
EP3113609B1 (en) 2014-03-05 2019-07-31 Novozymes A/S Methods for improving post-harvest properties of agricultural crops
WO2015134737A1 (en) 2014-03-05 2015-09-11 Novozymes A/S Compositions and methods for improving properties of cellulosic textile materials with xyloglucan endotransglycosylase
CN106062276B (zh) 2014-03-05 2019-06-11 诺维信公司 用于将材料功能化并且进行连接的组合物和方法
US20160348035A1 (en) 2014-03-05 2016-12-01 Novozymes A/S Compositions and Methods for Improving Properties of Non-Cellulosic Textile Materials with Xyloglucan Endotransglycosylase
CN106795504A (zh) 2014-08-20 2017-05-31 诺维信公司 木葡聚糖内糖基转移酶变体以及编码其的多核苷酸
US10781428B2 (en) 2014-12-02 2020-09-22 Novozymes A/S Laccase variants and polynucleotides encoding same
EA202092665A3 (ru) 2015-02-02 2021-06-30 МЕИРЭДжТиЭкс ЮКей II ЛИМИТЕД Регулирование экспрессии генов посредством аптамеропосредованного модулирования альтернативного сплайсинга
WO2016160584A1 (en) * 2015-03-27 2016-10-06 Cargill, Incorporated Glucoamylase-modified yeast strains and methods for bioproduct production
WO2016174258A1 (en) 2015-04-29 2016-11-03 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for protein production
CN108431229A (zh) 2015-12-17 2018-08-21 嘉吉公司 糖转运蛋白修饰的酵母菌株和用于生物制品生产的方法
MX2019001239A (es) 2016-08-03 2019-07-04 Meiragtx Uk Ii Ltd Deteccion de alto rendimiento basada en celulas para aptámeros.
US11421212B2 (en) 2016-08-05 2022-08-23 Cargill, Incorporated Engineered yeast strains with signal sequence-modified glucoamylase polypeptides and enhanced ethanol production
CN108949579B (zh) * 2018-04-19 2021-10-01 中国科学技术大学 嗜热子囊菌基因表达系统
US12398411B2 (en) 2018-06-15 2025-08-26 Kikkoman Corporation Method for producing selenoneine
WO2020025357A1 (en) 2018-08-02 2020-02-06 Novozymes A/S Preparation of combinatorial libraries of dna constructs using introns
EP3898985B1 (en) 2018-12-21 2023-02-08 Novozymes A/S Tandem protein expression
CN110592149B (zh) * 2019-07-16 2020-07-14 甘肃省科学院生物研究所 一种梨囊鞭菌发酵秸秆生产乳酸的新方法和应用
CN114391038A (zh) 2019-07-25 2022-04-22 诺维信公司 丝状真菌表达系统
US11479779B2 (en) * 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CN114292761B (zh) * 2021-12-02 2023-11-03 南京工业大学 一株黑曲霉基因工程菌及构建方法与应用
WO2025133003A2 (en) 2023-12-19 2025-06-26 21St.Bio A/S Non-animal bovine beta lactoglobulin
WO2025140482A1 (zh) * 2023-12-28 2025-07-03 南京百斯杰生物工程有限公司 可改善目标多肽表达效果的多核苷酸构建体及其应用
WO2025226596A1 (en) 2024-04-24 2025-10-30 Novozymes A/S Methods for producing secreted polypeptides

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BR112014029252A2 (pt) 2017-07-18
JP6449763B2 (ja) 2019-01-09
CN104395454A (zh) 2015-03-04
US20150175981A1 (en) 2015-06-25
US9487767B2 (en) 2016-11-08
JP2015517816A (ja) 2015-06-25
US10351861B2 (en) 2019-07-16
WO2013178674A1 (en) 2013-12-05
BR112014029252B8 (pt) 2022-01-25
EP2855659A1 (en) 2015-04-08
EP3327113A2 (en) 2018-05-30
DK3327113T3 (da) 2021-09-13
US10351863B2 (en) 2019-07-16
US20180298394A1 (en) 2018-10-18
BR112014029252B1 (pt) 2021-10-19
EP3327113B1 (en) 2021-08-18
US20170029830A1 (en) 2017-02-02
EP3327113A3 (en) 2018-08-08

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