BR112014029252A2 - método para a construção de uma célula hospedeira fúngica recombinante, construto de polinucleotídeo, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesse - Google Patents

método para a construção de uma célula hospedeira fúngica recombinante, construto de polinucleotídeo, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesse

Info

Publication number
BR112014029252A2
BR112014029252A2 BR112014029252A BR112014029252A BR112014029252A2 BR 112014029252 A2 BR112014029252 A2 BR 112014029252A2 BR 112014029252 A BR112014029252 A BR 112014029252A BR 112014029252 A BR112014029252 A BR 112014029252A BR 112014029252 A2 BR112014029252 A2 BR 112014029252A2
Authority
BR
Brazil
Prior art keywords
host cell
polynucleotide
fungal host
recombinant
recombinant fungal
Prior art date
Application number
BR112014029252A
Other languages
English (en)
Other versions
BR112014029252B1 (pt
BR112014029252B8 (pt
Inventor
Lillelund Olsen Carsten
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of BR112014029252A2 publication Critical patent/BR112014029252A2/pt
Publication of BR112014029252B1 publication Critical patent/BR112014029252B1/pt
Publication of BR112014029252B8 publication Critical patent/BR112014029252B8/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y107/00Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01003Nitrate reductase (NADPH) (1.7.1.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y107/00Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01004Nitrite reductase [NAD(P)H] (1.7.1.4)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/01Hydro-lyases (4.2.1)
    • C12Y402/01011Phosphopyruvate hydratase (4.2.1.11), i.e. enolase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1 / 1 resumo “mã‰todo para a construã‡ãƒo de uma cã‰lula hospedeira fãšngica recombinante, construto de polinucleotãdeo, cã‰lula hospedeira fãšngica recombinante, e, mã‰todo de produã‡ãƒo de um polipeptãdeo de interesse” a presente invenã§ã£o refere-se a mã©todos para a construã§ã£o de uma cã©lula hospedeira fãºngica recombinante compreendendo uma ou mais cã³pias de um construto de polinucleotã­deo integrado no seu genoma, o referido mã©todo compreendendo a transformaã§ã£o de uma cã©lula hospedeira fãºngica com um construto de polinucleotã­deo integrativo que compreende um primeiro polinucleotã­deo que codifica um marcador selecionã¡vel, caracterizado o primeiro polinucleotã­deo, uma regiã£o 5’ nã£o traduzida do mesmo e/ou um riboswitch operativamente ligado ã  mesma compreende um ã­ntron spliceosomal que tem 5 nucleotã­deos ou menos entre o seu sã­tio de ramificaã§ã£o e do seu sã­tio receptor; e um segundo polinucleotã­deo que codifica um polipeptã­deo de interesse; bem como construtos polinucleotã­dicos resultantes adequados, cã©lulas hospedeiras de fungos e mã©todos de fabricaã§ã£o.
BR112014029252A 2012-05-31 2013-05-29 Método para a construção de uma célula hospedeira fúngica recombinante, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesse BR112014029252B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12170260.9 2012-05-31
EP12170260 2012-05-31
PCT/EP2013/061052 WO2013178674A1 (en) 2012-05-31 2013-05-29 Improved selection in fungi

Publications (3)

Publication Number Publication Date
BR112014029252A2 true BR112014029252A2 (pt) 2017-07-18
BR112014029252B1 BR112014029252B1 (pt) 2021-10-19
BR112014029252B8 BR112014029252B8 (pt) 2022-01-25

Family

ID=48539153

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014029252A BR112014029252B8 (pt) 2012-05-31 2013-05-29 Método para a construção de uma célula hospedeira fúngica recombinante, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesse

Country Status (7)

Country Link
US (3) US9487767B2 (pt)
EP (2) EP2855659A1 (pt)
JP (1) JP6449763B2 (pt)
CN (1) CN104395454B (pt)
BR (1) BR112014029252B8 (pt)
DK (1) DK3327113T3 (pt)
WO (1) WO2013178674A1 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170073890A1 (en) 2014-03-05 2017-03-16 Novozymes A/S Compositions and Methods for Functionalizing and Linking Materials
WO2015134729A1 (en) 2014-03-05 2015-09-11 Novozymes A/S Compositions and methods for improving properties of non-cellulosic textile materials with xyloglucan endotransglycosylase
US20170027167A1 (en) 2014-03-05 2017-02-02 Novozymes Bioag A/S Formulations comprising polymeric xyloglucan as a carrier for agriculturally beneficial agents
CN106068077B (zh) 2014-03-05 2019-06-07 诺维信公司 用于改进农作物的收获后特性的组合物和方法
US20160333292A1 (en) 2014-03-05 2016-11-17 Novozymes A/S Compositions and Methods for Improving Properties of Cellulosic Textile Materials with Xyloglucan Endotransglycosylase
US20170267980A1 (en) 2014-08-20 2017-09-21 Novozymes A/S Xyloglucan Endotransglycosylase variants and Polynucleotides Encoding Same
US10781428B2 (en) 2014-12-02 2020-09-22 Novozymes A/S Laccase variants and polynucleotides encoding same
EA202092665A3 (ru) 2015-02-02 2021-06-30 МЕИРЭДжТиЭкс ЮКей II ЛИМИТЕД Регулирование экспрессии генов посредством аптамеропосредованного модулирования альтернативного сплайсинга
BR112017020474A2 (pt) * 2015-03-27 2018-07-03 Cargill Inc levedura modificada, meio de fermentação, método para produção de um bioproduto, ácido nucleico, vetor e método de fermentação para produzir etanol
WO2016174258A1 (en) 2015-04-29 2016-11-03 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for protein production
IL264490B2 (en) * 2016-08-03 2024-06-01 Meiragtx Uk Ii Ltd High-throughput cell-based screening for aptamers
EP3494129A1 (en) 2016-08-05 2019-06-12 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
CN108949579B (zh) * 2018-04-19 2021-10-01 中国科学技术大学 嗜热子囊菌基因表达系统
US20230159968A1 (en) * 2018-06-15 2023-05-25 Kikkoman Corporation Method for Producing Selenoneine
US20210301285A1 (en) 2018-08-02 2021-09-30 Novozymes A/S Preparation of Combinatorial Libraries of DNA Constructs
DK3898985T5 (da) 2018-12-21 2024-08-05 Novozymes As Proteinekspression i tandem
CN110592149B (zh) * 2019-07-16 2020-07-14 甘肃省科学院生物研究所 一种梨囊鞭菌发酵秸秆生产乳酸的新方法和应用
EP4004211A1 (en) 2019-07-25 2022-06-01 Novozymes A/S Filamentous fungal expression system
US11479779B2 (en) * 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CN114292761B (zh) * 2021-12-02 2023-11-03 南京工业大学 一株黑曲霉基因工程菌及构建方法与应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
CN1192108C (zh) 1994-06-03 2005-03-09 诺沃奇梅兹生物技术有限公司 纯化的毁丝霉属漆酶及编码该酶的核酸
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
EP0966521A1 (en) 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
AU6188599A (en) 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
EP2278016B1 (en) 1999-03-22 2012-09-26 Novozymes Inc. Promoter sequences derived from Fusarium Venenatum and uses thereof
WO2001090393A1 (en) * 2000-05-24 2001-11-29 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell
JP2007259787A (ja) * 2006-03-29 2007-10-11 Hakutsuru Shuzo Kk 遺伝子発現制御ポリヌクレオチド
GB201107768D0 (en) 2011-05-10 2011-06-22 Univ Manchester Riboswitches

Also Published As

Publication number Publication date
JP2015517816A (ja) 2015-06-25
WO2013178674A1 (en) 2013-12-05
EP2855659A1 (en) 2015-04-08
JP6449763B2 (ja) 2019-01-09
US20150175981A1 (en) 2015-06-25
BR112014029252B1 (pt) 2021-10-19
US10351863B2 (en) 2019-07-16
CN104395454A (zh) 2015-03-04
US10351861B2 (en) 2019-07-16
DK3327113T3 (da) 2021-09-13
EP3327113A3 (en) 2018-08-08
EP3327113B1 (en) 2021-08-18
US20170029830A1 (en) 2017-02-02
US9487767B2 (en) 2016-11-08
CN104395454B (zh) 2021-11-30
BR112014029252B8 (pt) 2022-01-25
US20180298394A1 (en) 2018-10-18
EP3327113A2 (en) 2018-05-30

Similar Documents

Publication Publication Date Title
BR112014029252A2 (pt) método para a construção de uma célula hospedeira fúngica recombinante, construto de polinucleotídeo, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesse
BR112015011466A2 (pt) célula hospedeira fúngica recombinante, constructo de ácido nucleico recombinante, sequência de polipeptídeo isolada, e, métodos para produzir um produto de fermentação e pelo menos um produto final a partir de pelo menos um substrato celulósico
BR112013009186A2 (pt) superexpressão de mirnas de plantas para o controle de parasitas
BR112013025753A8 (pt) Métodos para aprimoramento do rendimento de produto e produção em um micro-organismo através da adição de aceptores de elétrons alternativos
AR088252A1 (es) Produccion de una proteina de interes mediante un promotor regulable de pichia pastoris
EA201491470A1 (ru) Композиции фактора viii и способы получения и использования подобных
TR201903066T4 (tr) E. colı'de enzimatik konjugasyonla rekombinant aşı üretimi.
PE20151745A1 (es) Polipeptidos que tienen actividad de lisozima y polinucleotidos que los codifican
BR112016004413A2 (pt) composições, preparação de anticorpo e método de purificação de um polipeptídeo recombinante e de tratamento de um distúrbio
BR112014026206A2 (pt) sistemas e métodos celulares para melhorar a síntese de ácidos graxos através da expressão da desidrogenase
BR112012017396A2 (pt) sistema multifuncional de descongelamento / anticongelamento
BR112013023517A2 (pt) "inibidores de produção de leucotrieno, seus usos, e composição farmacêutica"
BR112015011389A2 (pt) Complexo f do vírus sincicial respiratório, métodos para produzir um complexo f do vírus sincicial respiratório e para indução de uma resposta imunológica a um f de rsv em um indivíduo, e, composição imunogênica
BR112015032418A2 (pt) métodos para produzir um laminado compósito e uma formação de camadas de laminado compósito, laminado compósito, e, formação de camadas de laminado compósito
BR112017010010A2 (pt) método para produzir 3-oxo-5-hidroxipentanoil-coa, método para biossintetizar 1,3-butanodiol, hospedeiro recombinante, produto bioderivado, produto biobaseado ou produto derivado de fermentação, retícula bioquímica de ocorrência não natural, construto de ácido nucleico ou vetor de expressão e composição
BR112014029708A8 (pt) Composto de pirrolo[2,1-f][1,2,4]triazina, método de preparação de compostos de pirrolo[2,1-f][1,2,4]triazina e uso do composto de pirrolo[2,1-f][1,2,4]triazina
BR112013017367A2 (pt) polipeptídeo de alfacarpo, sequência de ácido nucléico, composição farmacêutica e método para a produção de pelo menos um polipeptídeo de alfacorpo
BR112013004124A2 (pt) peptídeo ou complexo de peptídeos ligando a alfa2 integrina e métodos e utilizações envolvendo os mesmos
BR112013000059A2 (pt) derivados de piperidina e seus usos para o tratamento de distúrbios metabólicos
BR112012026907A2 (pt) método para a preparação de uma microvesícula suportando tf tendo atividade pró-coagulante, microvesículas obstidas pelo dito metodo e seu uso, bem como composição farmacêutica e kit as compreendendo
BR112016012371A2 (pt) produção microbial de aminas graxas
BR112012028031A2 (pt) célula hospedeira, e , métodos para produzir um ácido dicarboxílico c4 e para aumentar a produção de ácido dicarboxílico c4.
CR20140257A (es) Nuevos 2h-indazoles como antagonistas del receptor ep2
BR112014029095A2 (pt) célula, linhagem celular derivada de célula, polinucleotídeo isolado, método de preparar uma proteína de múltiplas subunidades, proteína de múltiplas subunidades, e, célula de mamífero ex vivo
BR112016002462A2 (pt) métodos para melhorar os sintomas da asma usando benralizumabe

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 29/05/2013, OBSERVADAS AS CONDICOES LEGAIS.

B09W Correction of the decision to grant [chapter 9.1.4 patent gazette]

Free format text: RETIFICACAO DO PARECER DE DEFERIMENTO DEVIDO A UMA INCORRECAO NO QUADRO 1.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: REFERENTE AO DESPACHO 16.1 PUBLICADO NA RPI 2650 DE 19.10.2021, QUANTO AOS DESENHOS