BR112014029252A2 - método para a construção de uma célula hospedeira fúngica recombinante, construto de polinucleotídeo, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesse - Google Patents
método para a construção de uma célula hospedeira fúngica recombinante, construto de polinucleotídeo, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesseInfo
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- C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
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- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01011—Phosphopyruvate hydratase (4.2.1.11), i.e. enolase
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Abstract
1 / 1 resumo âmãtodo para a construãão de uma cãlula hospedeira fãngica recombinante, construto de polinucleotãdeo, cãlula hospedeira fãngica recombinante, e, mãtodo de produãão de um polipeptãdeo de interesseâ a presente invenã§ã£o refere-se a mã©todos para a construã§ã£o de uma cã©lula hospedeira fãºngica recombinante compreendendo uma ou mais cã³pias de um construto de polinucleotãdeo integrado no seu genoma, o referido mã©todo compreendendo a transformaã§ã£o de uma cã©lula hospedeira fãºngica com um construto de polinucleotãdeo integrativo que compreende um primeiro polinucleotãdeo que codifica um marcador selecionã¡vel, caracterizado o primeiro polinucleotãdeo, uma regiã£o 5â nã£o traduzida do mesmo e/ou um riboswitch operativamente ligado ã mesma compreende um ãntron spliceosomal que tem 5 nucleotãdeos ou menos entre o seu sãtio de ramificaã§ã£o e do seu sãtio receptor; e um segundo polinucleotãdeo que codifica um polipeptãdeo de interesse; bem como construtos polinucleotãdicos resultantes adequados, cã©lulas hospedeiras de fungos e mã©todos de fabricaã§ã£o.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP12170260.9 | 2012-05-31 | ||
EP12170260 | 2012-05-31 | ||
PCT/EP2013/061052 WO2013178674A1 (en) | 2012-05-31 | 2013-05-29 | Improved selection in fungi |
Publications (3)
Publication Number | Publication Date |
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BR112014029252A2 true BR112014029252A2 (pt) | 2017-07-18 |
BR112014029252B1 BR112014029252B1 (pt) | 2021-10-19 |
BR112014029252B8 BR112014029252B8 (pt) | 2022-01-25 |
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ID=48539153
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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BR112014029252A BR112014029252B8 (pt) | 2012-05-31 | 2013-05-29 | Método para a construção de uma célula hospedeira fúngica recombinante, célula hospedeira fúngica recombinante, e, método de produção de um polipeptídeo de interesse |
Country Status (7)
Country | Link |
---|---|
US (3) | US9487767B2 (pt) |
EP (2) | EP2855659A1 (pt) |
JP (1) | JP6449763B2 (pt) |
CN (1) | CN104395454B (pt) |
BR (1) | BR112014029252B8 (pt) |
DK (1) | DK3327113T3 (pt) |
WO (1) | WO2013178674A1 (pt) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20170073890A1 (en) | 2014-03-05 | 2017-03-16 | Novozymes A/S | Compositions and Methods for Functionalizing and Linking Materials |
WO2015134729A1 (en) | 2014-03-05 | 2015-09-11 | Novozymes A/S | Compositions and methods for improving properties of non-cellulosic textile materials with xyloglucan endotransglycosylase |
US20170027167A1 (en) | 2014-03-05 | 2017-02-02 | Novozymes Bioag A/S | Formulations comprising polymeric xyloglucan as a carrier for agriculturally beneficial agents |
CN106068077B (zh) | 2014-03-05 | 2019-06-07 | 诺维信公司 | 用于改进农作物的收获后特性的组合物和方法 |
US20160333292A1 (en) | 2014-03-05 | 2016-11-17 | Novozymes A/S | Compositions and Methods for Improving Properties of Cellulosic Textile Materials with Xyloglucan Endotransglycosylase |
US20170267980A1 (en) | 2014-08-20 | 2017-09-21 | Novozymes A/S | Xyloglucan Endotransglycosylase variants and Polynucleotides Encoding Same |
US10781428B2 (en) | 2014-12-02 | 2020-09-22 | Novozymes A/S | Laccase variants and polynucleotides encoding same |
EA202092665A3 (ru) | 2015-02-02 | 2021-06-30 | МЕИРЭДжТиЭкс ЮКей II ЛИМИТЕД | Регулирование экспрессии генов посредством аптамеропосредованного модулирования альтернативного сплайсинга |
BR112017020474A2 (pt) * | 2015-03-27 | 2018-07-03 | Cargill Inc | levedura modificada, meio de fermentação, método para produção de um bioproduto, ácido nucleico, vetor e método de fermentação para produzir etanol |
WO2016174258A1 (en) | 2015-04-29 | 2016-11-03 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Means and methods for protein production |
IL264490B2 (en) * | 2016-08-03 | 2024-06-01 | Meiragtx Uk Ii Ltd | High-throughput cell-based screening for aptamers |
EP3494129A1 (en) | 2016-08-05 | 2019-06-12 | Cargill, Incorporated | Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production |
CN108949579B (zh) * | 2018-04-19 | 2021-10-01 | 中国科学技术大学 | 嗜热子囊菌基因表达系统 |
US20230159968A1 (en) * | 2018-06-15 | 2023-05-25 | Kikkoman Corporation | Method for Producing Selenoneine |
US20210301285A1 (en) | 2018-08-02 | 2021-09-30 | Novozymes A/S | Preparation of Combinatorial Libraries of DNA Constructs |
DK3898985T5 (da) | 2018-12-21 | 2024-08-05 | Novozymes As | Proteinekspression i tandem |
CN110592149B (zh) * | 2019-07-16 | 2020-07-14 | 甘肃省科学院生物研究所 | 一种梨囊鞭菌发酵秸秆生产乳酸的新方法和应用 |
EP4004211A1 (en) | 2019-07-25 | 2022-06-01 | Novozymes A/S | Filamentous fungal expression system |
US11479779B2 (en) * | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
CN114292761B (zh) * | 2021-12-02 | 2023-11-03 | 南京工业大学 | 一株黑曲霉基因工程菌及构建方法与应用 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
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DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
CN1192108C (zh) | 1994-06-03 | 2005-03-09 | 诺沃奇梅兹生物技术有限公司 | 纯化的毁丝霉属漆酶及编码该酶的核酸 |
ATE294871T1 (de) | 1994-06-30 | 2005-05-15 | Novozymes Biotech Inc | Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren |
EP0966521A1 (en) | 1996-09-13 | 1999-12-29 | Novo Nordisk Biotech, Inc. | Cells having dna insertion mutations which produce altered amounts of a polypeptide |
AU6188599A (en) | 1998-10-26 | 2000-05-15 | Novozymes A/S | Constructing and screening a dna library of interest in filamentous fungal cells |
EP2278016B1 (en) | 1999-03-22 | 2012-09-26 | Novozymes Inc. | Promoter sequences derived from Fusarium Venenatum and uses thereof |
WO2001090393A1 (en) * | 2000-05-24 | 2001-11-29 | Novozymes A/S | Method for increasing gene copy number in a host cell and resulting host cell |
JP2007259787A (ja) * | 2006-03-29 | 2007-10-11 | Hakutsuru Shuzo Kk | 遺伝子発現制御ポリヌクレオチド |
GB201107768D0 (en) | 2011-05-10 | 2011-06-22 | Univ Manchester | Riboswitches |
-
2013
- 2013-05-29 EP EP13726188.9A patent/EP2855659A1/en not_active Withdrawn
- 2013-05-29 DK DK17200169.5T patent/DK3327113T3/da active
- 2013-05-29 WO PCT/EP2013/061052 patent/WO2013178674A1/en active Application Filing
- 2013-05-29 EP EP17200169.5A patent/EP3327113B1/en active Active
- 2013-05-29 CN CN201380028411.5A patent/CN104395454B/zh active Active
- 2013-05-29 US US14/403,355 patent/US9487767B2/en active Active
- 2013-05-29 JP JP2015514483A patent/JP6449763B2/ja active Active
- 2013-05-29 BR BR112014029252A patent/BR112014029252B8/pt active Search and Examination
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2016
- 2016-10-18 US US15/296,433 patent/US10351861B2/en active Active
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2018
- 2018-06-26 US US16/018,726 patent/US10351863B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
JP2015517816A (ja) | 2015-06-25 |
WO2013178674A1 (en) | 2013-12-05 |
EP2855659A1 (en) | 2015-04-08 |
JP6449763B2 (ja) | 2019-01-09 |
US20150175981A1 (en) | 2015-06-25 |
BR112014029252B1 (pt) | 2021-10-19 |
US10351863B2 (en) | 2019-07-16 |
CN104395454A (zh) | 2015-03-04 |
US10351861B2 (en) | 2019-07-16 |
DK3327113T3 (da) | 2021-09-13 |
EP3327113A3 (en) | 2018-08-08 |
EP3327113B1 (en) | 2021-08-18 |
US20170029830A1 (en) | 2017-02-02 |
US9487767B2 (en) | 2016-11-08 |
CN104395454B (zh) | 2021-11-30 |
BR112014029252B8 (pt) | 2022-01-25 |
US20180298394A1 (en) | 2018-10-18 |
EP3327113A2 (en) | 2018-05-30 |
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