AR088252A1 - Produccion de una proteina de interes mediante un promotor regulable de pichia pastoris - Google Patents

Produccion de una proteina de interes mediante un promotor regulable de pichia pastoris

Info

Publication number
AR088252A1
AR088252A1 ARP120103723A ARP120103723A AR088252A1 AR 088252 A1 AR088252 A1 AR 088252A1 AR P120103723 A ARP120103723 A AR P120103723A AR P120103723 A ARP120103723 A AR P120103723A AR 088252 A1 AR088252 A1 AR 088252A1
Authority
AR
Argentina
Prior art keywords
promoter
poi
culturing
interest
protein
Prior art date
Application number
ARP120103723A
Other languages
English (en)
Inventor
Maurer Michael
Klein Joachim
Mattanovich Diethard
Wenger Jana
Gasser Brigitte
Prielhofer Roland
Original Assignee
Lonza Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Ag filed Critical Lonza Ag
Publication of AR088252A1 publication Critical patent/AR088252A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01012—Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

Un método de producción una proteína de interés (POI) cultivando una línea celular eucariota recombinante que comprende un constructo de expresión que comprende un promotor regulable de Pichia pastoris y una molécula de ácido nucleico que codifica una POI bajo el control de la transcripción de dicho promotor, que comprende las etapas de a) cultivar la línea celular con una fuente de carbón basal que reprima el promotor, b) cultivar la línea celular con una cantidad limitada de una fuente de carbono suplementaria que reprima el promotor para inducir la producción de POI a una tasa de transcripción de al menos el 15% en comparación con el promotor pGAP nativo, y c) producir y recuperar la POI; y también un promotor regulable aislado y un respectivo sistema de expresión. Acido nucleico y célula.
ARP120103723A 2011-10-07 2012-10-05 Produccion de una proteina de interes mediante un promotor regulable de pichia pastoris AR088252A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161544451P 2011-10-07 2011-10-07
EP11184323 2011-10-07
EP12171006 2012-06-06

Publications (1)

Publication Number Publication Date
AR088252A1 true AR088252A1 (es) 2014-05-21

Family

ID=48043194

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120103723A AR088252A1 (es) 2011-10-07 2012-10-05 Produccion de una proteina de interes mediante un promotor regulable de pichia pastoris

Country Status (17)

Country Link
US (4) US9512432B2 (es)
EP (2) EP2764016B1 (es)
JP (1) JP6113736B2 (es)
KR (2) KR102128957B1 (es)
CN (3) CN110055248A (es)
AR (1) AR088252A1 (es)
AU (1) AU2012320421B2 (es)
BR (1) BR112014008152A2 (es)
CA (1) CA2849856C (es)
DK (1) DK3508494T3 (es)
ES (1) ES2913825T3 (es)
IL (1) IL231859B (es)
IN (1) IN2014DN03419A (es)
PL (1) PL2764016T3 (es)
SG (2) SG11201400811QA (es)
TW (1) TWI560272B (es)
WO (1) WO2013050551A1 (es)

Families Citing this family (21)

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KR102128957B1 (ko) * 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
CN104195165A (zh) * 2014-09-01 2014-12-10 北京爱必信生物技术有限公司 包含葡萄糖启动子的载体、宿主菌以及重组牛源胰蛋白酶的制备方法
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
EP3332005B1 (en) * 2015-08-05 2021-09-22 Lonza Ltd Promoter variants
EP3423588A1 (en) 2016-03-02 2019-01-09 Lonza Ltd Improved fermentation process
WO2018110616A1 (ja) * 2016-12-15 2018-06-21 株式会社カネカ 新規宿主細胞及びそれを用いた目的タンパク質の製造方法
BR112020016258A2 (pt) * 2018-02-12 2020-12-15 Lonza Ltd Uma célula hospedeira eucariótica modificada geneticamente projetada para reduzir a produção de proteínas da célula hospedeira, método de produzir uma proteína de interesse usando a célula hospedeira, método para reduzir a contaminação por proteína da célula hospedeira
EP3536784A1 (en) 2018-03-05 2019-09-11 ACIB GmbH Host cell engineered for improved metabolite production
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TW202031898A (zh) 2019-01-11 2020-09-01 瑞士商隆沙有限公司 重組宿主細胞中的碳源調控蛋白質製造
EP3690052B1 (en) * 2019-01-29 2023-04-19 Clariant Produkte (Deutschland) GmbH Chimeric promoter for use in metabolic engineering
HUE064229T2 (hu) 2019-04-01 2024-02-28 Univ Wien Bodenkultur Mut-metilotróf élesztõ
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
EP3997118A4 (en) 2019-07-11 2023-07-19 Clara Foods Co. PROTEIN COMPOSITIONS AND CONSUMABLE PRODUCTS THEREOF
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
CN115698300A (zh) 2020-03-26 2023-02-03 阿斯克肋匹奥生物制药公司 佛司可林诱导型启动子和低氧诱导型启动子
EP4127185A1 (en) 2020-04-01 2023-02-08 Lonza Ltd. Helper factors for expressing proteins in yeast
EP4136237A1 (en) 2020-04-15 2023-02-22 Asklepios Biopharmaceutical, Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
CA3193155A1 (en) 2020-09-30 2022-04-07 Lonza Ltd Host cells overexpressing translational factors
JP2024506650A (ja) 2021-02-12 2024-02-14 ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 増加したタンパク質分泌のためのシグナルペプチド

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Also Published As

Publication number Publication date
EP3508494B1 (en) 2022-02-23
SG10201602673PA (en) 2016-05-30
US20220259608A1 (en) 2022-08-18
TWI560272B (en) 2016-12-01
EP3508494A1 (en) 2019-07-10
KR102128957B1 (ko) 2020-07-02
DK3508494T3 (da) 2022-05-23
KR101954389B1 (ko) 2019-03-06
US11401523B2 (en) 2022-08-02
SG11201400811QA (en) 2014-08-28
CN110055248A (zh) 2019-07-26
EP2764016A1 (en) 2014-08-13
CN103998460A (zh) 2014-08-20
AU2012320421B2 (en) 2017-03-02
EP2764016B1 (en) 2019-03-13
US20170037418A1 (en) 2017-02-09
KR20140069112A (ko) 2014-06-09
JP6113736B2 (ja) 2017-04-12
BR112014008152A2 (pt) 2017-04-18
TW201317350A (zh) 2013-05-01
JP2014530016A (ja) 2014-11-17
ES2913825T3 (es) 2022-06-06
KR20190025048A (ko) 2019-03-08
IL231859A0 (en) 2014-05-28
CA2849856A1 (en) 2013-04-11
IL231859B (en) 2020-08-31
US20140242636A1 (en) 2014-08-28
AU2012320421A1 (en) 2014-03-20
US20190185866A1 (en) 2019-06-20
PL2764016T3 (pl) 2019-08-30
US11976284B2 (en) 2024-05-07
US10301634B2 (en) 2019-05-28
CN113788884A (zh) 2021-12-14
US9512432B2 (en) 2016-12-06
WO2013050551A1 (en) 2013-04-11
CA2849856C (en) 2023-03-14
IN2014DN03419A (es) 2015-05-22

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