KR100717353B1 - 피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법 - Google Patents

피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법 Download PDF

Info

Publication number
KR100717353B1
KR100717353B1 KR1020060033064A KR20060033064A KR100717353B1 KR 100717353 B1 KR100717353 B1 KR 100717353B1 KR 1020060033064 A KR1020060033064 A KR 1020060033064A KR 20060033064 A KR20060033064 A KR 20060033064A KR 100717353 B1 KR100717353 B1 KR 100717353B1
Authority
KR
South Korea
Prior art keywords
recombinant protein
promoter
auto
pichia pastoris
sodium phosphate
Prior art date
Application number
KR1020060033064A
Other languages
English (en)
Inventor
이홍원
안정오
정준기
최의성
김천석
이은교
홍지연
이혁원
박명수
Original Assignee
한국생명공학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국생명공학연구원 filed Critical 한국생명공학연구원
Priority to KR1020060033064A priority Critical patent/KR100717353B1/ko
Priority to AT06783531T priority patent/ATE535605T1/de
Priority to CN2006800541805A priority patent/CN101415824B/zh
Priority to US12/294,511 priority patent/US8080389B2/en
Priority to PCT/KR2006/003096 priority patent/WO2007117062A1/en
Priority to JP2009505270A priority patent/JP4830018B2/ja
Priority to EP06783531A priority patent/EP2013342B1/en
Application granted granted Critical
Publication of KR100717353B1 publication Critical patent/KR100717353B1/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020060033064A 2006-04-12 2006-04-12 피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법 KR100717353B1 (ko)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR1020060033064A KR100717353B1 (ko) 2006-04-12 2006-04-12 피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법
AT06783531T ATE535605T1 (de) 2006-04-12 2006-08-07 Autoinduzierbarer natriumphosphat-symporter- promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon
CN2006800541805A CN101415824B (zh) 2006-04-12 2006-08-07 获自毕赤酵母的自动诱导型磷酸钠协同转运体启动子和利用该启动子制造重组蛋白的方法
US12/294,511 US8080389B2 (en) 2006-04-12 2006-08-07 Auto-inducible sodium phosphate symporter promoter from Pichia pastoris and method for producing recombinant protein using it
PCT/KR2006/003096 WO2007117062A1 (en) 2006-04-12 2006-08-07 Auto- inducible sodium phosphate symporter promoter from pichia pastoris and method for producing recombinant protein using it
JP2009505270A JP4830018B2 (ja) 2006-04-12 2006-08-07 ピキア・パストリス由来の自動誘導性ｎｐｓプロモーター及びそれを用いた異種タンパク質の製造方法
EP06783531A EP2013342B1 (en) 2006-04-12 2006-08-07 Auto- inducible sodium phosphate symporter promoter from pichia pastoris and method for producing recombinant protein using it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020060033064A KR100717353B1 (ko) 2006-04-12 2006-04-12 피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법

Publications (1)

Publication Number Publication Date
KR100717353B1 true KR100717353B1 (ko) 2007-05-11

Family

ID=38270490

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020060033064A KR100717353B1 (ko) 2006-04-12 2006-04-12 피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법

Country Status (7)

Country Link
US (1) US8080389B2 (ko)
EP (1) EP2013342B1 (ko)
JP (1) JP4830018B2 (ko)
KR (1) KR100717353B1 (ko)
CN (1) CN101415824B (ko)
AT (1) ATE535605T1 (ko)
WO (1) WO2007117062A1 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
US10104183B2 (en) * 2010-06-22 2018-10-16 Microsoft Technology Licensing, Llc Networked device authentication, pairing and resource sharing
EP3508494B1 (en) 2011-10-07 2022-02-23 Lonza Ltd Regulatable promoter
CN106148339B (zh) * 2015-04-24 2019-01-04 中国科学院大连化学物理研究所 一种Na+/Pi协同转运蛋白启动子和终止子及其应用
CN106148338B (zh) * 2015-04-24 2019-01-04 中国科学院大连化学物理研究所 Na+/Pi协同转运蛋白启动子和终止子及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9501939D0 (sv) * 1995-05-24 1995-05-24 Astra Ab DNA molecules for expression of polypeptides
US7816509B2 (en) * 2005-11-16 2010-10-19 Korean Research Institute Of Bioscience & Biotechnology Translational elongation factor promoter from Pichia pastoris and method for producing recombinant protein using the same

Also Published As

Publication number Publication date
CN101415824A (zh) 2009-04-22
JP4830018B2 (ja) 2011-12-07
JP2009533049A (ja) 2009-09-17
EP2013342A1 (en) 2009-01-14
US20100279345A1 (en) 2010-11-04
WO2007117062A1 (en) 2007-10-18
EP2013342B1 (en) 2011-11-30
CN101415824B (zh) 2012-02-29
EP2013342A4 (en) 2009-07-29
US8080389B2 (en) 2011-12-20
ATE535605T1 (de) 2011-12-15

Similar Documents

Publication Publication Date Title
MY160897A (en) Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof
MX2011011215A (es) Polipeptido que degrada carbohidratos y usos del mismo.
EA200901110A1 (ru) Рекомбинантная клетка-хозяин для получения соединения, представляющего интерес
MX2012015142A (es) Polipeptidos que tiene actividad beta-glucosidasa y usos del mismo.
MX2013003681A (es) Ácidos nucleicos manipulados y métodos de uso de los mismos.
MX2008000200A (es) Polinucleotidos que codifican enzimas que modifican el isoprenoide y sus metodos de uso.
MX2010008099A (es) Anticuerpos y angiopoyetina-2 estabilizados y sus usos.
MX2010002753A (es) Plantas que tienen rasgos aumentados relacionados con el rendimiento y un metodo para obtenerlas.
NZ584893A (en) PORCINE DC-SIGN, ICAM-3 AND LSECtin AND USES THEREOF
MX2008010669A (es) Plantas con caracteristicas mejoradas y un metodo para prepararlas.
MX2009010610A (es) Dominio de enlace especifico de especies cruzadas.
UA110599C2 (uk) Пептид для імунотерапії пухлин, включаючи нейрональні пухлини та пухлини мозку
BRPI0808104A8 (pt) Métodos para preparar e para produzir um polipeptídeo, polipeptídeo, sequência de ácido nucleico isolada, construção de ácido nucleico, vetor de expressão recombinante, e, célula hospedeira recombinante
MX351565B (es) Colageno 7 y metodos relacionados.
WO2008023247A3 (en) Matrix attachment regions (mars) for increasing transcription and uses thereof
ATE546464T1 (de) Verfahren zur produktion von rekombinantem protein in cho-zellen
MX2010000385A (es) Clon celular para producir la fsh.
KR100717353B1 (ko) 피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법
PE20090683A1 (es) Peptidos de senalizacion
WO2009066320A3 (en) Gcsf fusion protein systems suitable for high expression of peptides
ES2531402T3 (es) Colagenasa de fusión a la que se une una marca de afinidad, y método para producir la misma
EP1951877A4 (en) TRANSLATIONAL ELONGATION FACTOR PROMOTER FROM PICHIA PASTORIS AND PROCESS FOR PRODUCING RECOMBINANT PROTEIN THEREWITH
WO2007130543A3 (en) Method for producing stable mammalian cell lines producing high levels of recombinant proteins
DE602005017034D1 (de) Signalpeptid zur herstellung eines polypeptids
BRPI1006628B8 (pt) método para produzir uma enzima lipolítica

Legal Events

Date Code Title Description
A201 Request for examination
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20130430

Year of fee payment: 7

FPAY Annual fee payment

Payment date: 20140502

Year of fee payment: 8

FPAY Annual fee payment

Payment date: 20150427

Year of fee payment: 9

FPAY Annual fee payment

Payment date: 20160504

Year of fee payment: 10

FPAY Annual fee payment

Payment date: 20170605

Year of fee payment: 11

LAPS Lapse due to unpaid annual fee