HRP20240183T1 - Stanični probir visoke propusnosti za aptamere - Google Patents
Stanični probir visoke propusnosti za aptamere Download PDFInfo
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- HRP20240183T1 HRP20240183T1 HRP20240183TT HRP20240183T HRP20240183T1 HR P20240183 T1 HRP20240183 T1 HR P20240183T1 HR P20240183T T HRP20240183T T HR P20240183TT HR P20240183 T HRP20240183 T HR P20240183T HR P20240183 T1 HRP20240183 T1 HR P20240183T1
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- exon
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- intron
- riboswitch
- reporter gene
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- 108091023037 Aptamer Proteins 0.000 title claims 12
- 238000012216 screening Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims 12
- 108020004422 Riboswitch Proteins 0.000 claims 11
- 108700008625 Reporter Genes Proteins 0.000 claims 9
- 210000003527 eukaryotic cell Anatomy 0.000 claims 9
- 239000003446 ligand Substances 0.000 claims 9
- 230000000295 complement effect Effects 0.000 claims 6
- 108091033319 polynucleotide Proteins 0.000 claims 6
- 102000040430 polynucleotide Human genes 0.000 claims 6
- 239000002157 polynucleotide Substances 0.000 claims 6
- 230000014509 gene expression Effects 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 239000013612 plasmid Substances 0.000 claims 4
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 2
- 108020004705 Codon Proteins 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims 2
- 101000908713 Homo sapiens Dihydrofolate reductase Proteins 0.000 claims 2
- 108020005067 RNA Splice Sites Proteins 0.000 claims 2
- 230000001580 bacterial effect Effects 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims 2
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims 2
- 239000012636 effector Substances 0.000 claims 2
- 239000003623 enhancer Substances 0.000 claims 2
- 238000005259 measurement Methods 0.000 claims 2
- 230000001404 mediated effect Effects 0.000 claims 2
- 108020004999 messenger RNA Proteins 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 claims 1
- 102100025228 Calcium/calmodulin-dependent protein kinase type II subunit delta Human genes 0.000 claims 1
- 108090000695 Cytokines Proteins 0.000 claims 1
- 102000004127 Cytokines Human genes 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 claims 1
- 108010051696 Growth Hormone Proteins 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 101001077338 Homo sapiens Calcium/calmodulin-dependent protein kinase type II subunit delta Proteins 0.000 claims 1
- 101000899111 Homo sapiens Hemoglobin subunit beta Proteins 0.000 claims 1
- 101000621309 Homo sapiens Wilms tumor protein Proteins 0.000 claims 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims 1
- 108091092195 Intron Proteins 0.000 claims 1
- 108060001084 Luciferase Proteins 0.000 claims 1
- 239000005089 Luciferase Substances 0.000 claims 1
- 102100031455 NAD-dependent protein deacetylase sirtuin-1 Human genes 0.000 claims 1
- 238000012408 PCR amplification Methods 0.000 claims 1
- 108010041191 Sirtuin 1 Proteins 0.000 claims 1
- 102100038803 Somatotropin Human genes 0.000 claims 1
- 108010022394 Threonine synthase Proteins 0.000 claims 1
- 108010005774 beta-Galactosidase Proteins 0.000 claims 1
- 102000005936 beta-Galactosidase Human genes 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 125000003636 chemical group Chemical group 0.000 claims 1
- 102000004419 dihydrofolate reductase Human genes 0.000 claims 1
- 108091006047 fluorescent proteins Proteins 0.000 claims 1
- 102000034287 fluorescent proteins Human genes 0.000 claims 1
- 239000000122 growth hormone Substances 0.000 claims 1
- 102000046004 human WT1 Human genes 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000002207 metabolite Substances 0.000 claims 1
- 150000002772 monosaccharides Chemical class 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 230000011664 signaling Effects 0.000 claims 1
- -1 signaling molecule Proteins 0.000 claims 1
- 230000003584 silencer Effects 0.000 claims 1
- 150000003384 small molecules Chemical class 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 230000001225 therapeutic effect Effects 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 150000003722 vitamin derivatives Chemical class 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3517—Marker; Tag
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3519—Fusion with another nucleic acid
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- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
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- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
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Claims (12)
1. Postupak za odabir aptomera koji veže ligand u eukariotskim stanicama koji sadrži sljedeće korake:
(a) pružanje biblioteke aptamera,
(b) uvođenje članova biblioteke aptamera u polinukleotidnu kazetu za ekspresiju reporterskog gena posredovanu ligandom kako bi se stvorila biblioteka riboprekidača,
(c) uvođenje biblioteke riboprekidača u eukariotske stanice, i
(d) dovođenje u kontakt eukariotskih stanica s ligandom, i
(e) mjerenje ekspresije reporterskog gena,
naznačen time što polinukleotidna kazeta sadrži alternativno spojeni egzon, okružen 5' intronom i 3' intronom, i riboprekidač koji sadrži (i) efektorsku regiju koja sadrži stabljiku koja uključuje sekvencu 5' mjesta spajanja 3' introna i sekvencu komplementarnu sekvenci 5' mjesta spajanja 3' introna, i (ii) aptamer smješten između sekvence 5' mjesta spajanja 3' introna i komplementarne sekvence, pri čemu alternativno spojeni egzon sadrži stop kodon koji je u okviru s reporterskim genom kada je alternativno spojeni egzon spojen u mRNA reporterskog gena.
2. Postupak prema zahtjevu 1, naznačen time što je biblioteka aptamera podijeljena u manju biblioteku aptamera prije uvođenja u polinukleotidne kazete koje obuhvaćaju korake:
(a) pružanje randomizirane biblioteke aptamera pri čemu aptameri u biblioteci sadrže višestruke 5' i 3' konstantne regije i jedan ili više randomiziranih nukleotida,
(b) izvođenje PCR-a s dva ciklusa korištenjem biblioteke randomiziranih aptamera kao predloška te prve početnice i druge početnice koje su komplementarne 5' i 3' konstantnim regijama,
(c) izolaciju produkata dvociklusne PCR, i
(d) PCR pojačavanje podskupa izoliranih produkata dvociklične PCR pomoću početnica komplementarnih podskupu jedinstvenih 5' i 3' konstantnih regija.
3. Postupak prema zahtjevu 1 ili 2, naznačen time što je biblioteka riboprekidača podijeljena u jednu ili više podbiblioteka riboprekidača prije uvođenja u eukariotske stanice, izborno, pri čemu je biblioteka riboprekidača dalje podijeljena u podbiblioteke koje sadrže koraci od:
(a) uvođenje biblioteke riboprekidač u bakterije;
(b) prikupljanje bakterijskih klonova i ekstrakcija plazmidne DNK radi dobivanja plazmidnih podbiblioteka riboprekidača za stvaranje jedne ili više primarnih podbiblioteka;
(c) izborno, generiranje sekundarne podbiblioteke riboprekidača iz primarne plazmidne podbiblioteke riboprekidača uvođenjem primarne podbiblioteke u bakterije, prikupljanjem bakterijskih klonova i izolacijom plazmidne DNK.
4. Postupak za odabir aptomera koji veže ligand u eukariotskim stanicama koji sadrži sljedeće korake:
(a) pružanje biblioteke liganada,
(b) pružanje polinukleotidne kazete za ekspresiju reporterskog gena posredovanu ligandom,
(c) uvođenje polinukleotidne kasete u eukariotsku stanicu,
(d) dovođenje u kontakt pojedinačnih skupina eukariotskih stanica s članovima biblioteke liganada, i
(e) mjerenje ekspresije reporterskog gena,
pri čemu polinukleotidna kazeta sadrži alternativno spojeni egzon, okružen 5' intronom i 3' intronom, i riboprekidač koji sadrži (i) efektorsku regiju koja sadrži stabljiku koja uključuje sekvencu 5' mjesta spajanja 3' introna i sekvencu komplementarnu sekvenci 5' mjesta spajanja 3' introna, i (ii) aptamer smješten između sekvence 5' mjesta spajanja 3' introna i komplementarne sekvence, pri čemu alternativno spojeni egzon sadrži stop kodon koji je u okviru s reporterskim genom kada je alternativno spojeni egzon spojen u mRNA reporterskog gena.
5. Postupak prema bilo kojem od zahtjeva 1, 3 ili 4, naznačen time što je ligand
(a) mala molekula; ili
(b) molekula koju proizvodi eukariotska stanica odabrana iz skupine koja se sastoji od metabolita, nukleinske kiseline, vitamina, kofaktora, lipida, monosaharida i drugog glasnika.
6. Postupak prema bilo kojem od zahtjeva 1, 3 ili 4, naznačen time što je eukariotska stanica odabrana iz grupe koju čine stanica sisavca, stanica insekta, biljna stanica i stanica kvasca.
7. Postupak prema bilo kojem od zahtjeva 1, 3 ili 4, naznačen time što je reporterski gen odabran iz skupine koja se sastoji od
(a) fluorescentnog proteina, luciferaza, β-galaktozidaza i peroksidaza hrena; ili
(b) citokina, signalne molekule, hormona rasta, antitijela, regulatorne RNK, terapijskog proteina ili peptida.
8. Postupak prema bilo kojem od zahtjeva 1, 3 ili 4, naznačen time što su 5’ i 3’ introni
(a) izvedeni iz introna 2 ljudskog gena za β-globin;
(b) svaki neovisno dužine od 50 do 300 nukleotida; ili
(c) svaki neovisno dužine od 125 do 240 nukleotida.
9. Postupak prema bilo kojem od zahtjeva 1, 3 ili 4, naznačen time što je ligand
(a) 7 do 20 baznih parova u duljinu; ili
(b) 8 do 11 baznih parova u duljinu.
10. Postupak prema bilo kojem od zahtjeva 1, 3 ili 4, naznačen time što alternativno spojeni egzon
(a) potječe iz skupine koja se sastoji od eksona 2 gena za ljudsku dihidrofolat reduktazu (DHFR), mutiranog ljudskog Wilmsovog tumora 1 eksona 5, mišjeg kalcij/kalmodulina ovisne protein kinaze II delta egzona 16, i SIRT1 egzona 6;
(b) je modificirani egzon 2 iz ljudskog DHFR;
(c) nije izveden iz prirodnog egzona; ili
(d) sadrži jednu ili više skupina koje se sastoje od izmijenjenog pojačivača egzonskog spoja, izmijenjenog prigušivača egzonskog spoja, dodanog pojačivača egzonskog spoja, i dodanog prigušivača egzonskog spoja.
11. Postupak prema zahtjevu 1 ili zahtjevu 3, naznačen time što biblioteka aptamera sadrži aptamere koji imaju jedan ili više randomiziranih nukleotida.
12. Postupak prema zahtjevu 2 ili zahtjevu 3, naznačen time što prva ili druga početnica u PCR-u s dva ciklusa zadrži oznaku odabranu iz skupine koja se sastoji od biotina, digoksigenina (DIG), bromodeoksiuridina (BrdU), fluorofora i kemijske skupine koja se upotrebljava u kemijskom ciklusu klikova.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662370599P | 2016-08-03 | 2016-08-03 | |
| PCT/IB2017/001113 WO2018025085A2 (en) | 2016-08-03 | 2017-08-03 | High throughput cell-based screening for aptamers |
| EP17790843.1A EP3494229B1 (en) | 2016-08-03 | 2017-08-03 | High throughput cell-based screening for aptamers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20240183T1 true HRP20240183T1 (hr) | 2024-04-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20240183TT HRP20240183T1 (hr) | 2016-08-03 | 2017-08-03 | Stanični probir visoke propusnosti za aptamere |
Country Status (22)
| Country | Link |
|---|---|
| US (2) | US11371077B2 (hr) |
| EP (1) | EP3494229B1 (hr) |
| JP (2) | JP7089502B2 (hr) |
| KR (1) | KR102491566B1 (hr) |
| CN (1) | CN110199030B (hr) |
| AU (2) | AU2017306657B2 (hr) |
| BR (1) | BR112019002085A2 (hr) |
| CA (1) | CA3066654C (hr) |
| DK (1) | DK3494229T3 (hr) |
| EA (1) | EA201990423A1 (hr) |
| FI (1) | FI3494229T3 (hr) |
| HR (1) | HRP20240183T1 (hr) |
| IL (1) | IL264490B1 (hr) |
| LT (1) | LT3494229T (hr) |
| MX (1) | MX2019001239A (hr) |
| MY (1) | MY196527A (hr) |
| PH (1) | PH12019500239A1 (hr) |
| PL (1) | PL3494229T3 (hr) |
| PT (1) | PT3494229T (hr) |
| RS (1) | RS65228B1 (hr) |
| SG (1) | SG11201900851QA (hr) |
| WO (1) | WO2018025085A2 (hr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180117630A (ko) * | 2016-02-02 | 2018-10-29 | 메이라지티엑스 유케이 Ii 리미티드 | 압타머-조절된 폴리아데닐화를 통한 유전자 발현의 조절 |
| KR102645079B1 (ko) | 2017-02-21 | 2024-03-08 | 메이라지티엑스 유케이 Ii 리미티드 | 폴리아데닐화 신호의 압타머-매개된 접근성에 의한 유전자 발현의 조절 |
| WO2019165067A1 (en) | 2018-02-21 | 2019-08-29 | Bristol-Myers Squibb Company | Camk2d antisense oligonucleotides and uses thereof |
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| US5580737A (en) | 1990-06-11 | 1996-12-03 | Nexstar Pharmaceuticals, Inc. | High-affinity nucleic acid ligands that discriminate between theophylline and caffeine |
| US5567588A (en) | 1990-06-11 | 1996-10-22 | University Research Corporation | Systematic evolution of ligands by exponential enrichment: Solution SELEX |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| JP2763958B2 (ja) | 1990-06-11 | 1998-06-11 | ネクスター ファーマスーティカルズ，インコーポレイテッド | 核酸リガンド |
| US6423493B1 (en) * | 1998-10-26 | 2002-07-23 | Board Of Regents The University Of Texas System | Combinatorial selection of oligonucleotide aptamers |
| US8008071B2 (en) * | 1999-11-08 | 2011-08-30 | University Of South Florida | Compositions and methods for detecting intracellular glucose and analogs thereof |
| US20040126882A1 (en) * | 2000-06-15 | 2004-07-01 | Ellington Andrew D | Regulatable, catalytically active nucleic acids |
| WO2004028464A2 (en) | 2002-09-27 | 2004-04-08 | University Of North Carolina At Chapel Hill | Methods and compositions for modification of splicing of pre-mrna |
| JP2008518630A (ja) | 2004-11-08 | 2008-06-05 | イェール ユニバーシティ | リボスイッチ、リボスイッチによる、構造を基礎とする化合物設計、ならびにリボスイッチの使用方法およびリボスイッチを伴う組成物 |
| WO2006084868A2 (en) * | 2005-02-09 | 2006-08-17 | Basf Plant Science Gmbh | Expression cassettes for regulation of expression in monocotyledonous plants |
| WO2007035532A2 (en) * | 2005-09-15 | 2007-03-29 | Duke University | Focused libraries, functional profiling, laser selex and deselex |
| WO2008116220A2 (en) | 2007-03-22 | 2008-09-25 | Yale University | Methods and compositions related to riboswitches that control alternative splicing |
| KR20100017893A (ko) | 2007-05-29 | 2010-02-16 | 예일 유니버시티 | 택일적 스플라이싱 및 ｒｎａ 프로세싱을 조절하는 리보스위치와 관련된 방법 및 조성물 |
| US8604176B2 (en) * | 2009-11-10 | 2013-12-10 | California Institute Of Technology | Protein-responsive RNA control devices and uses thereof |
| DK3327113T3 (da) * | 2012-05-31 | 2021-09-13 | Novozymes As | Forbedret svampeselektion |
| AU2014205648B2 (en) * | 2013-01-10 | 2017-05-04 | Dharmacon, Inc. | Templates, libraries, kits and methods for generating molecules |
| PT3265563T (pt) | 2015-02-02 | 2021-06-03 | Meiragtx Uk Ii Ltd | Regulação da expressão genética por modulação mediada por aptâmero de splicing alternativo |
| GB201506440D0 (en) | 2015-04-16 | 2015-06-03 | Univ Wageningen | Riboswitch-controlled screening and selection of desired biocatalysts |
| US10011870B2 (en) * | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
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