SG11201900851QA - High throughput cell-based screening for aptamers - Google Patents
High throughput cell-based screening for aptamersInfo
- Publication number
- SG11201900851QA SG11201900851QA SG11201900851QA SG11201900851QA SG11201900851QA SG 11201900851Q A SG11201900851Q A SG 11201900851QA SG 11201900851Q A SG11201900851Q A SG 11201900851QA SG 11201900851Q A SG11201900851Q A SG 11201900851QA SG 11201900851Q A SG11201900851Q A SG 11201900851QA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- aptamer
- ligand
- publication
- based screening
- Prior art date
Links
- 108091023037 Aptamer Proteins 0.000 title abstract 6
- 238000012216 screening Methods 0.000 title abstract 3
- 239000003446 ligand Substances 0.000 abstract 3
- 108700008625 Reporter Genes Proteins 0.000 abstract 2
- 108020004422 Riboswitch Proteins 0.000 abstract 2
- 230000014509 gene expression Effects 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- ZAZHPBXBNGWCDV-UHFFFAOYSA-N N2-Maltulosylarginine Chemical compound OC1C(O)C(CNC(CCCNC(=N)N)C(O)=O)(O)OCC1OC1C(O)C(O)C(O)C(CO)O1 ZAZHPBXBNGWCDV-UHFFFAOYSA-N 0.000 abstract 1
- 239000012636 effector Substances 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3517—Marker; Tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3519—Fusion with another nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/30—Production chemically synthesised
- C12N2330/31—Libraries, arrays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
Abstract
GCO r•i O 1-1 r•i C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 February 2018 (08.02.2018) WIPOIPCT omit Ill °nolo 01101 OH 100 (10) International Publication Number WO 2018/025085 A3 (51) International Patent Classification: C12Q 1/68 (2018.01) Cl 2N 15/115 (2010.01) C12N 15/10 (2006.01) Cl 2N 15/113 (2010.01) (21) International Application Number: PCT/IB2017/001113 (22) International Filing Date: 03 August 2017 (03.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/370,599 03 August 2016 (03.08.2016) US (71) Applicant: MEIRAGTX UK II LIMITED [GB/GB]; 92 Britannia Walk, London N1 7NQ (GB). (72) Inventors: GUO, Xuecui; 1 Bungtown Road, Cold Spring Harbor, NY 11724 (US). FENG, Lei; 1207 Neill Avenue, Bronx, NY 10461 (US). FORBES, Alexandria; 14 East 10th Street, Apt 2, New York, NY 10003 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) (88) Date of publication of the international search report: 12 April 2018 (12.04.2018) (54) Title: HIGH THROUGHPUT CELL-BASED SCREENING FOR APTAMERS Aptamer U1 binding site- N. Exonl mDHFR site-complementary E) i - cor) FIG. la (57) : The invention provides eukaryotic cell-based screening methods to identify an aptamer that specifically binds a ligand, or a ligand that specifically binds an aptamer, using a polynucleotide cassette for the regulation of the expression of a reporter gene where the polynucleotide cassette contains a riboswitch in the context of a 5' intron-alternative exon-3' intron. The riboswitch comprises an effector region and an aptamer such that when the aptamer binds a ligand, reporter gene expression occurs.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662370599P | 2016-08-03 | 2016-08-03 | |
| PCT/IB2017/001113 WO2018025085A2 (en) | 2016-08-03 | 2017-08-03 | High throughput cell-based screening for aptamers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201900851QA true SG11201900851QA (en) | 2019-02-27 |
Family
ID=60182820
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201900851QA SG11201900851QA (en) | 2016-08-03 | 2017-08-03 | High throughput cell-based screening for aptamers |
Country Status (21)
| Country | Link |
|---|---|
| US (2) | US11371077B2 (en) |
| EP (1) | EP3494229B1 (en) |
| JP (2) | JP7089502B2 (en) |
| KR (1) | KR102491566B1 (en) |
| CN (1) | CN110199030B (en) |
| AU (2) | AU2017306657B2 (en) |
| BR (1) | BR112019002085A2 (en) |
| CA (1) | CA3066654C (en) |
| DK (1) | DK3494229T3 (en) |
| EA (1) | EA201990423A1 (en) |
| FI (1) | FI3494229T3 (en) |
| IL (1) | IL264490B1 (en) |
| LT (1) | LT3494229T (en) |
| MX (1) | MX2019001239A (en) |
| MY (1) | MY196527A (en) |
| PH (1) | PH12019500239A1 (en) |
| PL (1) | PL3494229T3 (en) |
| PT (1) | PT3494229T (en) |
| RS (1) | RS65228B1 (en) |
| SG (1) | SG11201900851QA (en) |
| WO (1) | WO2018025085A2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3048620A1 (en) * | 2016-02-02 | 2017-08-10 | Meiragtx Uk Ii Limited | Regulation of gene expression through aptamer-modulated polyadenylation |
| WO2018156658A1 (en) | 2017-02-21 | 2018-08-30 | MEIRAGTX, UK II Limited | Regulation of gene expression by aptamer-mediated accessibility of polyadenylation signals |
| SG11202007652UA (en) | 2018-02-21 | 2020-09-29 | Bristol Myers Squibb Co | Camk2d antisense oligonucleotides and uses thereof |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| US5567588A (en) | 1990-06-11 | 1996-10-22 | University Research Corporation | Systematic evolution of ligands by exponential enrichment: Solution SELEX |
| US5580737A (en) | 1990-06-11 | 1996-12-03 | Nexstar Pharmaceuticals, Inc. | High-affinity nucleic acid ligands that discriminate between theophylline and caffeine |
| CA2084987C (en) | 1990-06-11 | 2007-02-13 | Larry Gold | Nucleic acid ligands |
| US6423493B1 (en) * | 1998-10-26 | 2002-07-23 | Board Of Regents The University Of Texas System | Combinatorial selection of oligonucleotide aptamers |
| US8008071B2 (en) | 1999-11-08 | 2011-08-30 | University Of South Florida | Compositions and methods for detecting intracellular glucose and analogs thereof |
| US20040126882A1 (en) * | 2000-06-15 | 2004-07-01 | Ellington Andrew D | Regulatable, catalytically active nucleic acids |
| EP1546733A2 (en) | 2002-09-27 | 2005-06-29 | University Of North Carolina At Chapel Hill | Methods and compositions for modification of splicing of pre-mrna |
| CA2586998A1 (en) | 2004-11-08 | 2006-05-26 | Yale University | Structure-based compound design involving riboswitches |
| WO2006084868A2 (en) * | 2005-02-09 | 2006-08-17 | Basf Plant Science Gmbh | Expression cassettes for regulation of expression in monocotyledonous plants |
| EP1931803A2 (en) * | 2005-09-15 | 2008-06-18 | Duke University | Focused libraries, functional profiling, laser selex and deselex |
| JP2010521977A (en) * | 2007-03-22 | 2010-07-01 | イェール ユニバーシティー | Methods and compositions for riboswitches that regulate alternative splicing |
| JP2010528616A (en) * | 2007-05-29 | 2010-08-26 | イェール ユニバーシティー | Methods and compositions related to riboswitches that regulate alternative splicing and RNA splicing |
| US8604176B2 (en) | 2009-11-10 | 2013-12-10 | California Institute Of Technology | Protein-responsive RNA control devices and uses thereof |
| WO2013178674A1 (en) | 2012-05-31 | 2013-12-05 | Novozymes A/S | Improved selection in fungi |
| EP2943579B1 (en) * | 2013-01-10 | 2018-09-12 | Dharmacon, Inc. | Libraries and methods for generating molecules |
| PL3265563T3 (en) * | 2015-02-02 | 2021-09-13 | Meiragtx Uk Ii Limited | Regulation of gene expression by aptamer-mediated modulation of alternative splicing |
| GB201506440D0 (en) * | 2015-04-16 | 2015-06-03 | Univ Wageningen | Riboswitch-controlled screening and selection of desired biocatalysts |
| US10011870B2 (en) * | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
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2017
- 2017-08-03 EA EA201990423A patent/EA201990423A1/en unknown
- 2017-08-03 MX MX2019001239A patent/MX2019001239A/en unknown
- 2017-08-03 KR KR1020197005510A patent/KR102491566B1/en active IP Right Grant
- 2017-08-03 US US16/322,865 patent/US11371077B2/en active Active
- 2017-08-03 PL PL17790843.1T patent/PL3494229T3/en unknown
- 2017-08-03 RS RS20240097A patent/RS65228B1/en unknown
- 2017-08-03 EP EP17790843.1A patent/EP3494229B1/en active Active
- 2017-08-03 PT PT177908431T patent/PT3494229T/en unknown
- 2017-08-03 IL IL264490A patent/IL264490B1/en unknown
- 2017-08-03 WO PCT/IB2017/001113 patent/WO2018025085A2/en active Application Filing
- 2017-08-03 DK DK17790843.1T patent/DK3494229T3/en active
- 2017-08-03 MY MYPI2019000618A patent/MY196527A/en unknown
- 2017-08-03 LT LTEPPCT/IB2017/001113T patent/LT3494229T/en unknown
- 2017-08-03 BR BR112019002085A patent/BR112019002085A2/en unknown
- 2017-08-03 CN CN201780061565.2A patent/CN110199030B/en active Active
- 2017-08-03 AU AU2017306657A patent/AU2017306657B2/en active Active
- 2017-08-03 FI FIEP17790843.1T patent/FI3494229T3/en active
- 2017-08-03 CA CA3066654A patent/CA3066654C/en active Active
- 2017-08-03 JP JP2019505401A patent/JP7089502B2/en active Active
- 2017-08-03 SG SG11201900851QA patent/SG11201900851QA/en unknown
-
2019
- 2019-02-01 PH PH12019500239A patent/PH12019500239A1/en unknown
-
2022
- 2022-06-09 JP JP2022093742A patent/JP2022113773A/en active Pending
- 2022-06-27 US US17/809,043 patent/US20230065720A1/en active Pending
-
2023
- 2023-03-08 AU AU2023201431A patent/AU2023201431A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018025085A3 (en) | 2018-04-12 |
| AU2023201431A1 (en) | 2023-04-13 |
| CN110199030A (en) | 2019-09-03 |
| WO2018025085A2 (en) | 2018-02-08 |
| IL264490A (en) | 2019-02-28 |
| FI3494229T3 (en) | 2024-01-29 |
| PL3494229T3 (en) | 2024-03-25 |
| DK3494229T3 (en) | 2024-01-29 |
| PH12019500239A1 (en) | 2019-07-29 |
| US20200239940A1 (en) | 2020-07-30 |
| IL264490B1 (en) | 2024-02-01 |
| CA3066654C (en) | 2023-07-18 |
| AU2017306657B2 (en) | 2022-12-08 |
| BR112019002085A2 (en) | 2019-08-06 |
| RS65228B1 (en) | 2024-03-29 |
| EP3494229A2 (en) | 2019-06-12 |
| JP2022113773A (en) | 2022-08-04 |
| US11371077B2 (en) | 2022-06-28 |
| CA3066654A1 (en) | 2018-02-08 |
| MY196527A (en) | 2023-04-18 |
| EP3494229B1 (en) | 2023-10-25 |
| JP2019523013A (en) | 2019-08-22 |
| NZ750917A (en) | 2023-08-25 |
| KR20190058461A (en) | 2019-05-29 |
| US20230065720A1 (en) | 2023-03-02 |
| PT3494229T (en) | 2024-02-20 |
| EA201990423A1 (en) | 2019-09-30 |
| MX2019001239A (en) | 2019-07-04 |
| LT3494229T (en) | 2024-02-12 |
| KR102491566B1 (en) | 2023-01-26 |
| JP7089502B2 (en) | 2022-06-22 |
| CN110199030B (en) | 2023-12-12 |
| AU2017306657A1 (en) | 2019-03-14 |
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