EA201990423A1 - Высокопроизводительный клеточный скрининг на аптамеры - Google Patents

Высокопроизводительный клеточный скрининг на аптамеры

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Publication number
EA201990423A1
EA201990423A1 EA201990423A EA201990423A EA201990423A1 EA 201990423 A1 EA201990423 A1 EA 201990423A1 EA 201990423 A EA201990423 A EA 201990423A EA 201990423 A EA201990423 A EA 201990423A EA 201990423 A1 EA201990423 A1 EA 201990423A1
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EA
Eurasian Patent Office
Prior art keywords
aptamer
ligand
high performance
performance cell
cell camera
Prior art date
Application number
EA201990423A
Other languages
English (en)
Inventor
Сюэцуй Го
Лэй Фэн
Александрия Форбс
Original Assignee
Мейраджитиэкс Юкэй Айай Лимитед
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Application filed by Мейраджитиэкс Юкэй Айай Лимитед filed Critical Мейраджитиэкс Юкэй Айай Лимитед
Publication of EA201990423A1 publication Critical patent/EA201990423A1/ru

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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    • C12N2330/00—Production
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides

Abstract

Данное изобретение обеспечивает способы скрининга на основе эукариотических клеток для идентификации аптамера, который специфически связывает лиганд, или лиганда, который специфически связывает аптамер, с использованием полинуклеотидной кассеты для регуляции экспрессии репортерного гена, причем полинуклеотидная кассета содержит рибопереключатель в контексте 5' интрона - альтернативного экзона - 3' интрона. Указанный рибопереключатель содержит эффекторную область и аптамер таким образом, что, когда аптамер связывает лиганд, происходит экспрессия репортерного гена.
EA201990423A 2016-08-03 2017-08-03 Высокопроизводительный клеточный скрининг на аптамеры EA201990423A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662370599P 2016-08-03 2016-08-03
PCT/IB2017/001113 WO2018025085A2 (en) 2016-08-03 2017-08-03 High throughput cell-based screening for aptamers

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EA201990423A1 true EA201990423A1 (ru) 2019-09-30

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EA201990423A EA201990423A1 (ru) 2016-08-03 2017-08-03 Высокопроизводительный клеточный скрининг на аптамеры

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US (2) US11371077B2 (ru)
EP (1) EP3494229B1 (ru)
JP (2) JP7089502B2 (ru)
KR (1) KR102491566B1 (ru)
CN (1) CN110199030B (ru)
AU (2) AU2017306657B2 (ru)
BR (1) BR112019002085A2 (ru)
CA (1) CA3066654C (ru)
DK (1) DK3494229T3 (ru)
EA (1) EA201990423A1 (ru)
FI (1) FI3494229T3 (ru)
HR (1) HRP20240183T1 (ru)
IL (1) IL264490B1 (ru)
LT (1) LT3494229T (ru)
MX (1) MX2019001239A (ru)
MY (1) MY196527A (ru)
PH (1) PH12019500239A1 (ru)
PL (1) PL3494229T3 (ru)
PT (1) PT3494229T (ru)
RS (1) RS65228B1 (ru)
SG (1) SG11201900851QA (ru)
WO (1) WO2018025085A2 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112018015819A2 (pt) 2016-02-02 2019-01-02 Meiragtx Uk Ii Ltd regulação da expressão gênica por bloqueio de poliadenilação mediado por aptâmero
WO2018156658A1 (en) 2017-02-21 2018-08-30 MEIRAGTX, UK II Limited Regulation of gene expression by aptamer-mediated accessibility of polyadenylation signals
EP3755800A1 (en) 2018-02-21 2020-12-30 Bristol-Myers Squibb Company Camk2d antisense oligonucleotides and uses thereof

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5567588A (en) 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
ES2259800T3 (es) 1990-06-11 2006-10-16 Gilead Sciences, Inc. Procedimientos de uso de ligandos de acido nucleico.
US5580737A (en) 1990-06-11 1996-12-03 Nexstar Pharmaceuticals, Inc. High-affinity nucleic acid ligands that discriminate between theophylline and caffeine
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US6423493B1 (en) * 1998-10-26 2002-07-23 Board Of Regents The University Of Texas System Combinatorial selection of oligonucleotide aptamers
US8008071B2 (en) * 1999-11-08 2011-08-30 University Of South Florida Compositions and methods for detecting intracellular glucose and analogs thereof
US20040126882A1 (en) * 2000-06-15 2004-07-01 Ellington Andrew D Regulatable, catalytically active nucleic acids
JP2006500933A (ja) 2002-09-27 2006-01-12 ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル プレｍＲＮＡのスプライシングを修飾するための方法および組成物
MX2007005491A (es) 2004-11-08 2007-09-27 Univ Yale Diseno de compuesto basado en la estructura que involucra riboconmutadores.
CN103184218A (zh) * 2005-02-09 2013-07-03 巴斯福植物科学有限公司 在单子叶植物中调控表达的表达盒
EP1931803A2 (en) * 2005-09-15 2008-06-18 Duke University Focused libraries, functional profiling, laser selex and deselex
EP2471925A1 (en) 2007-03-22 2012-07-04 Yale University Methods and compositions related to riboswitches that control alternative splicing
CN101688251A (zh) 2007-05-29 2010-03-31 耶鲁大学 与控制可变剪接和rna加工的核糖开关有关的方法和组合物
US8604176B2 (en) 2009-11-10 2013-12-10 California Institute Of Technology Protein-responsive RNA control devices and uses thereof
EP3327113B1 (en) 2012-05-31 2021-08-18 Novozymes A/S Improved selection in fungi
LT2943579T (lt) * 2013-01-10 2018-11-12 Dharmacon, Inc. Molekulių bibliotekos ir molekulių generavimo būdai
AU2016215454B2 (en) * 2015-02-02 2022-05-19 Meiragtx Uk Ii Limited Regulation of gene expression by aptamer-mediated modulation of alternative splicing
GB201506440D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Riboswitch-controlled screening and selection of desired biocatalysts
US10011870B2 (en) * 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules

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JP2019523013A (ja) 2019-08-22
MX2019001239A (es) 2019-07-04
FI3494229T3 (fi) 2024-01-29
IL264490B1 (en) 2024-02-01
LT3494229T (lt) 2024-02-12
US20230065720A1 (en) 2023-03-02
AU2023201431A1 (en) 2023-04-13
KR20190058461A (ko) 2019-05-29
EP3494229B1 (en) 2023-10-25
BR112019002085A2 (pt) 2019-08-06
DK3494229T3 (da) 2024-01-29
EP3494229A2 (en) 2019-06-12
HRP20240183T1 (hr) 2024-04-26
PH12019500239A1 (en) 2019-07-29
US20200239940A1 (en) 2020-07-30
CN110199030A (zh) 2019-09-03
AU2017306657B2 (en) 2022-12-08
PL3494229T3 (pl) 2024-03-25
IL264490A (en) 2019-02-28
US11371077B2 (en) 2022-06-28
JP7089502B2 (ja) 2022-06-22
KR102491566B1 (ko) 2023-01-26
CN110199030B (zh) 2023-12-12
MY196527A (en) 2023-04-18
WO2018025085A3 (en) 2018-04-12
CA3066654A1 (en) 2018-02-08
PT3494229T (pt) 2024-02-20
CA3066654C (en) 2023-07-18
AU2017306657A1 (en) 2019-03-14
JP2022113773A (ja) 2022-08-04
WO2018025085A2 (en) 2018-02-08
NZ750917A (en) 2023-08-25
RS65228B1 (sr) 2024-03-29
SG11201900851QA (en) 2019-02-27

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