BR112014014037B8 - "construção de ácido nucleico e polinucleotídeo de fita dupla sintético compreendendo elemento de núcleo mínimo de promotor e um promotor do vírus baciliforme da cana-de-açúcar (scbv)" - Google Patents

"construção de ácido nucleico e polinucleotídeo de fita dupla sintético compreendendo elemento de núcleo mínimo de promotor e um promotor do vírus baciliforme da cana-de-açúcar (scbv)"

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Publication number
BR112014014037B8
BR112014014037B8 BR112014014037A BR112014014037A BR112014014037B8 BR 112014014037 B8 BR112014014037 B8 BR 112014014037B8 BR 112014014037 A BR112014014037 A BR 112014014037A BR 112014014037 A BR112014014037 A BR 112014014037A BR 112014014037 B8 BR112014014037 B8 BR 112014014037B8
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BR
Brazil
Prior art keywords
promoter
polynucleotide
nucleic acid
stanned
scbv
Prior art date
Application number
BR112014014037A
Other languages
English (en)
Other versions
BR112014014037A2 (pt
BR112014014037B1 (pt
Inventor
Kumar Sandeep
Alabed Diaa
Wright Terry
Gupta Manju
Original Assignee
Dow Agrosciences Llc
Corteva Agriscience Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Dow Agrosciences Llc, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Publication of BR112014014037A2 publication Critical patent/BR112014014037A2/pt
Publication of BR112014014037B1 publication Critical patent/BR112014014037B1/pt
Publication of BR112014014037B8 publication Critical patent/BR112014014037B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

POLINUCLEOTÍDEO COMPREENDENDO ELEMENTO PROMOTOR DE NÚCLEO MÍNIMO DE GENE DE UBIQUITINA-1 DE ZEA MAYS OU ZEA LUXURIANS , BEM COMO CONSTRUTO DE ÁCIDO NUCLÉICO E VETOR BINÁRIO, E MÉTODOS PARA PRODUÇÃO DE CÉLULA E PLANTA TRANSGÊNICAS E PARA EXPRESSÃO DE MÚLTIPLOS GENES NAS MESMAS. A presente invenção refere-se a polinucleotídeo sintético compreendendo elemento promotor de núcleo mínimo de gene de Ubiquitina-1 de Zea mays ou Zea luxurians , a construto de ácido nucléico para expressão de múltiplos genes em células e/ou tecidos de plantas, e a vetor binário para transformação mediada por Agrobacterium. A presente invenção refere-se ainda ao método para a produção de célula transgênica utilizando o dito polinucleotídeo e para gerar planta ou célula transgênica, bem como a método para a expressão de sequência de nucleotídeos de interesse em célula de planta e para a expressão de múltiplos genes em células e/ou tecidos de plantas.
BR112014014037A 2011-12-30 2012-11-12 "construção de ácido nucleico e polinucleotídeo de fita dupla sintético compreendendo elemento de núcleo mínimo de promotor e um promotor do vírus baciliforme da cana-de-açúcar (scbv)" BR112014014037B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161582138P 2011-12-30 2011-12-30
US61/582,138 2011-12-30
US201261617252P 2012-03-29 2012-03-29
US61/617,252 2012-03-29
PCT/US2012/064683 WO2013101343A1 (en) 2011-12-30 2012-11-12 Construct and method for synthetic bidirectional plant promoter ubi1

Publications (3)

Publication Number Publication Date
BR112014014037A2 BR112014014037A2 (pt) 2020-11-03
BR112014014037B1 BR112014014037B1 (pt) 2022-06-21
BR112014014037B8 true BR112014014037B8 (pt) 2023-05-16

Family

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BR112014014037A BR112014014037B8 (pt) 2011-12-30 2012-11-12 "construção de ácido nucleico e polinucleotídeo de fita dupla sintético compreendendo elemento de núcleo mínimo de promotor e um promotor do vírus baciliforme da cana-de-açúcar (scbv)"

Country Status (16)

Country Link
US (1) US9422569B2 (pt)
EP (1) EP2797406B1 (pt)
JP (1) JP6220794B2 (pt)
KR (1) KR20140107334A (pt)
CN (1) CN104135851B (pt)
AR (1) AR089510A1 (pt)
AU (1) AU2012363062B2 (pt)
BR (1) BR112014014037B8 (pt)
CA (1) CA2855902C (pt)
HK (1) HK1201121A1 (pt)
IL (1) IL233452A0 (pt)
RU (1) RU2639538C2 (pt)
SA (1) SA112340162B1 (pt)
TW (1) TWI601739B (pt)
WO (1) WO2013101343A1 (pt)
ZA (1) ZA201402992B (pt)

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BR102014021330A2 (pt) 2013-08-30 2015-09-22 Dow Agrosciences Llc construtos para expressão de transgenes usando elementos reguladores de genes de ubiquitina de panicum
TW201527313A (zh) * 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（二）
TW201527314A (zh) 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（三）
TW201527312A (zh) 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（一）
TW201527316A (zh) * 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（五）
TW201619386A (zh) * 2014-11-11 2016-06-01 陶氏農業科學公司 合成性雙向植物啓動子
AR102612A1 (es) * 2014-11-11 2017-03-15 Dow Agrosciences Llc Promotor vegetal bidireccional sintético
US10563220B2 (en) * 2015-12-21 2020-02-18 Monsanto Technology Llc Compositions and methods for efficient targeting of transgenes
CN105624163B (zh) * 2016-04-06 2018-07-06 中国农业科学院生物技术研究所 一种来源于棉花的双向启动子及其应用
CN106676107B (zh) * 2016-04-28 2019-04-05 华中农业大学 水稻内源双向表达启动子的分离克隆及功能分析

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Also Published As

Publication number Publication date
EP2797406A1 (en) 2014-11-05
CA2855902A1 (en) 2013-07-04
ZA201402992B (en) 2015-11-25
RU2639538C2 (ru) 2017-12-21
EP2797406B1 (en) 2020-01-08
AU2012363062A1 (en) 2014-05-15
EP2797406A4 (en) 2015-07-15
IL233452A0 (en) 2014-08-31
AR089510A1 (es) 2014-08-27
CN104135851B (zh) 2018-05-01
RU2014131448A (ru) 2016-02-20
US9422569B2 (en) 2016-08-23
BR112014014037A2 (pt) 2020-11-03
BR112014014037B1 (pt) 2022-06-21
CA2855902C (en) 2022-06-21
US20130254943A1 (en) 2013-09-26
SA112340162B1 (ar) 2016-02-22
JP2015503342A (ja) 2015-02-02
JP6220794B2 (ja) 2017-10-25
HK1201121A1 (en) 2015-08-28
AU2012363062B2 (en) 2018-04-12
KR20140107334A (ko) 2014-09-04
CN104135851A (zh) 2014-11-05
WO2013101343A1 (en) 2013-07-04
TW201329097A (zh) 2013-07-16
TWI601739B (zh) 2017-10-11

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