BR112012016807A2 - cepa de hospedeiro bacteriano que compreende um gene spr mutante e um gene tsp do tipo selvagem - Google Patents

cepa de hospedeiro bacteriano que compreende um gene spr mutante e um gene tsp do tipo selvagem

Info

Publication number
BR112012016807A2
BR112012016807A2 BR112012016807A BR112012016807A BR112012016807A2 BR 112012016807 A2 BR112012016807 A2 BR 112012016807A2 BR 112012016807 A BR112012016807 A BR 112012016807A BR 112012016807 A BR112012016807 A BR 112012016807A BR 112012016807 A2 BR112012016807 A2 BR 112012016807A2
Authority
BR
Brazil
Prior art keywords
gene
wild type
mutant spr
host strain
bacterial host
Prior art date
Application number
BR112012016807A
Other languages
English (en)
Other versions
BR112012016807B1 (pt
BR112012016807A8 (pt
Inventor
Paul Humphreys David
Ellis Mark
Original Assignee
Ucb Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Pharma Sa filed Critical Ucb Pharma Sa
Publication of BR112012016807A2 publication Critical patent/BR112012016807A2/pt
Publication of BR112012016807A8 publication Critical patent/BR112012016807A8/pt
Publication of BR112012016807B1 publication Critical patent/BR112012016807B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C12N1/28—Processes using, or culture media containing, hydrocarbons aliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

cepa de hospedeiro bacteriano que compreende um gene spr mutante e um gene tsp do tipo selvagem. a presente invenção proporciona uma célula bacteriana gram negativa recombinante que compreende um gene spr mutante que codifica uma proteína spr mutante e em que a célula compreende um gene tsp cromossômico do tipo selvagem não recombinante.
BR112012016807A 2010-01-14 2011-01-13 cepa de hospedeiro bacteriano que compreende um gene spr mutante e um gene tsp do tipo selvagem BR112012016807B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1000590.8A GB201000590D0 (en) 2010-01-14 2010-01-14 Bacterial host strain
PCT/EP2011/050415 WO2011086138A1 (en) 2010-01-14 2011-01-13 Bacterial host strain comprising a mutant spr gene and a wild-type tsp gene

Publications (3)

Publication Number Publication Date
BR112012016807A2 true BR112012016807A2 (pt) 2017-06-27
BR112012016807A8 BR112012016807A8 (pt) 2019-08-06
BR112012016807B1 BR112012016807B1 (pt) 2019-09-10

Family

ID=42028360

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012016807A BR112012016807B1 (pt) 2010-01-14 2011-01-13 cepa de hospedeiro bacteriano que compreende um gene spr mutante e um gene tsp do tipo selvagem

Country Status (23)

Country Link
US (2) US8969037B2 (pt)
EP (1) EP2523975B1 (pt)
JP (1) JP5960607B2 (pt)
KR (1) KR101741865B1 (pt)
CN (1) CN102712694B (pt)
AU (2) AU2011206586B2 (pt)
BR (1) BR112012016807B1 (pt)
CA (1) CA2785931C (pt)
CY (1) CY1122174T1 (pt)
DK (1) DK2523975T3 (pt)
ES (1) ES2747981T3 (pt)
GB (1) GB201000590D0 (pt)
HK (1) HK1176942A1 (pt)
HR (1) HRP20191815T1 (pt)
HU (1) HUE046420T2 (pt)
LT (1) LT2523975T (pt)
ME (1) ME03561B (pt)
PL (1) PL2523975T3 (pt)
PT (1) PT2523975T (pt)
RS (1) RS59417B1 (pt)
SG (1) SG182279A1 (pt)
SI (1) SI2523975T1 (pt)
WO (1) WO2011086138A1 (pt)

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GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201001791D0 (en) 2010-02-03 2010-03-24 Ucb Pharma Sa Process for obtaining antibodies
GB201012599D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
CN103649124B (zh) * 2011-07-13 2016-04-13 Ucb医药有限公司 表达重组dsbc的细菌宿主菌株
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CN110055202B (zh) * 2019-03-15 2023-08-22 百奥泰生物制药股份有限公司 用于高表达外源蛋白的大肠杆菌及其构建方法与应用
CN114829578A (zh) * 2019-08-05 2022-07-29 瓦克化学股份公司 用于在发酵方法中释放重组蛋白的细菌菌株

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Also Published As

Publication number Publication date
US9493558B2 (en) 2016-11-15
US20150166651A1 (en) 2015-06-18
CA2785931C (en) 2019-07-30
US20120301920A1 (en) 2012-11-29
AU2011206586A1 (en) 2012-07-19
BR112012016807B1 (pt) 2019-09-10
HRP20191815T1 (hr) 2019-12-27
HUE046420T2 (hu) 2020-03-30
US8969037B2 (en) 2015-03-03
LT2523975T (lt) 2019-09-25
ME03561B (me) 2020-07-20
AU2011206586B2 (en) 2016-10-27
AU2017200545B2 (en) 2018-10-25
KR20120125569A (ko) 2012-11-15
WO2011086138A1 (en) 2011-07-21
ES2747981T3 (es) 2020-03-12
CA2785931A1 (en) 2011-07-21
BR112012016807A8 (pt) 2019-08-06
DK2523975T3 (da) 2019-09-23
RS59417B1 (sr) 2019-11-29
EP2523975B1 (en) 2019-07-17
SI2523975T1 (sl) 2019-10-30
KR101741865B1 (ko) 2017-05-30
AU2017200545A1 (en) 2017-02-23
CN102712694B (zh) 2015-09-09
EP2523975A1 (en) 2012-11-21
CY1122174T1 (el) 2020-11-25
GB201000590D0 (en) 2010-03-03
JP5960607B2 (ja) 2016-08-02
SG182279A1 (en) 2012-08-30
JP2013516965A (ja) 2013-05-16
PT2523975T (pt) 2019-10-14
HK1176942A1 (zh) 2013-08-09
PL2523975T3 (pl) 2020-01-31
CN102712694A (zh) 2012-10-03

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