BR112012016808A2 - cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida - Google Patents

cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida

Info

Publication number
BR112012016808A2
BR112012016808A2 BR112012016808A BR112012016808A BR112012016808A2 BR 112012016808 A2 BR112012016808 A2 BR 112012016808A2 BR 112012016808 A BR112012016808 A BR 112012016808A BR 112012016808 A BR112012016808 A BR 112012016808A BR 112012016808 A2 BR112012016808 A2 BR 112012016808A2
Authority
BR
Brazil
Prior art keywords
host strain
bacterial host
expressing recombinant
strain expressing
reduced tsp
Prior art date
Application number
BR112012016808A
Other languages
English (en)
Inventor
Paul Humphreys David
Ellis Mark
Original Assignee
Ucb Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Pharma Sa filed Critical Ucb Pharma Sa
Publication of BR112012016808A2 publication Critical patent/BR112012016808A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/04—Intramolecular oxidoreductases (5.3) transposing S-S bonds (5.3.4)
    • C12Y503/04001—Protein disulfide-isomerase (5.3.4.1), i.e. disufide bond-forming enzyme
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

cepa de hospedeiro bacteriano que compreende um gene spr mutante e um gene tsp do tipo selvagem. a presente invenção fornece uma célula bacteriana gram negativa recombinante, em que a célula: a. compreende um polinucleotídeo recombinante que codifica dsbc; e b. possui atividade de proteína tsp reduzida comparada à célula do tipo selvagem.
BR112012016808A 2010-01-14 2011-01-13 cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida BR112012016808A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1000591.6A GB201000591D0 (en) 2010-01-14 2010-01-14 Bacterial hoist strain
PCT/EP2011/050416 WO2011086139A1 (en) 2010-01-14 2011-01-13 Bacterial host strain expressing recombinant dsbc and having reduced tsp activity

Publications (1)

Publication Number Publication Date
BR112012016808A2 true BR112012016808A2 (pt) 2017-06-27

Family

ID=42028361

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012016808A BR112012016808A2 (pt) 2010-01-14 2011-01-13 cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida

Country Status (23)

Country Link
US (2) US8969038B2 (pt)
EP (1) EP2523969B1 (pt)
JP (1) JP5960608B2 (pt)
KR (1) KR101729361B1 (pt)
CN (2) CN104328079A (pt)
AU (2) AU2011206587A1 (pt)
BR (1) BR112012016808A2 (pt)
CA (1) CA2786336C (pt)
CY (1) CY1118990T1 (pt)
DK (1) DK2523969T3 (pt)
ES (1) ES2627826T3 (pt)
GB (1) GB201000591D0 (pt)
HK (2) HK1176943A1 (pt)
HR (1) HRP20170852T1 (pt)
HU (1) HUE033555T2 (pt)
LT (1) LT2523969T (pt)
ME (1) ME02752B (pt)
PL (1) PL2523969T3 (pt)
PT (1) PT2523969T (pt)
RS (1) RS56159B1 (pt)
SG (1) SG182280A1 (pt)
SI (1) SI2523969T1 (pt)
WO (1) WO2011086139A1 (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2012DN02401A (pt) 2009-09-24 2015-08-21 Ucb Pharma Sa
GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000591D0 (en) * 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000590D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201001791D0 (en) 2010-02-03 2010-03-24 Ucb Pharma Sa Process for obtaining antibodies
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
BR112013032871B1 (pt) 2011-07-13 2022-09-27 Ucb Pharma S.A. Cepa hospedeira bacteriana que expressa dsbc recombinante e método para produzir anticorpo ou fragmento de ligação de antígeno deste que se liga especificamente a cd154
EP2546267A1 (en) * 2011-07-13 2013-01-16 UCB Pharma S.A. Bacterial host strain expressing recombinant DsbC
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
US10563225B2 (en) 2013-07-26 2020-02-18 President And Fellows Of Harvard College Genome engineering
EP3237432B1 (en) 2014-12-22 2023-09-06 UCB Biopharma SRL Protein manufacture
BR112018015259A2 (pt) 2016-01-27 2018-12-18 Medimmune Llc métodos para preparação de anticorpos com um padrão de glicosilação definido
AU2019375413A1 (en) * 2018-11-05 2021-05-27 Genentech, Inc. Methods of producing two chain proteins in prokaryotic host cells
EP3942079A1 (en) 2019-03-18 2022-01-26 Bio-Rad ABD Serotec GmbH Protection of spytag-containing periplasmic fusion proteins from protease tsp and ompt degradation

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264365A (en) 1990-11-09 1993-11-23 Board Of Regents, The University Of Texas System Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides
US5508192A (en) 1990-11-09 1996-04-16 Board Of Regents, The University Of Texas System Bacterial host strains for producing proteolytically sensitive polypeptides
GB9215540D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5304472A (en) 1992-11-20 1994-04-19 Genentech, Inc. Method of controlling polypeptide production in bacterial cells
US5639635A (en) 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
US6027888A (en) 1996-04-05 2000-02-22 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells
US6083715A (en) 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
JP2000083670A (ja) 1998-09-09 2000-03-28 Hsp Kenkyusho:Kk ＤｓｂＡ／ＤｓｂＢ／ＤｓｂＣ／ＤｓｂＤ発現プラスミド
EP1190258B1 (en) 1999-06-29 2006-10-25 Siga Technologies, Inc. Assay for degp protease inhibitors
GB0006398D0 (en) 2000-03-16 2000-05-03 Novartis Ag Organic compounds
GB0013810D0 (en) 2000-06-06 2000-07-26 Celltech Chiroscience Ltd Biological products
WO2002018446A2 (en) 2000-09-01 2002-03-07 Biogen, Inc. Methods of designing and producing compounds having improved binding affinity for cd154 or other trimeric proteins
AU8867501A (en) 2000-09-01 2002-03-13 Biogen Inc Co-crystal structure of monoclonal antibody 5c8 and cd154, and use thereof in drug design
US7041479B2 (en) 2000-09-06 2006-05-09 The Board Of Trustess Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
DK1341899T3 (da) 2000-12-14 2006-04-10 Genentech Inc Bakterieværtsstammer
KR100879194B1 (ko) * 2000-12-14 2009-01-16 제넨테크, 인크. 원핵생물에 의해 제조된 항체 및 이의 용도
TWI334439B (en) 2001-08-01 2010-12-11 Centocor Inc Anti-tnf antibodies, compositions, methods and uses
MXPA04001566A (es) 2001-08-27 2004-05-17 Genentech Inc Un sistema para expresion y ensamblado de anticuerpos.
GB0124317D0 (en) 2001-10-10 2001-11-28 Celltech R&D Ltd Biological products
WO2003048306A2 (en) 2001-11-16 2003-06-12 Idec Pharmaceuticals Corporation Polycistronic expression of antibodies
GB0129105D0 (en) 2001-12-05 2002-01-23 Celltech R&D Ltd Expression control using variable intergenic sequences
ATE328906T1 (de) 2002-06-28 2006-06-15 Domantis Ltd Dual-specifische liganden mit erhöhter halbwertszeit
GB0224082D0 (en) 2002-10-16 2002-11-27 Celltech R&D Ltd Biological products
MXPA05004677A (es) 2002-10-31 2005-11-17 Genentech Inc Metodos y composiciones para aumentar la produccion de anticuerpos.
US7993864B2 (en) 2002-12-03 2011-08-09 Ucb Pharma S.A. Assay for identifying antibody producing cells
GB0303337D0 (en) 2003-02-13 2003-03-19 Celltech R&D Ltd Biological products
AU2004253868B2 (en) 2003-06-13 2011-06-16 Biogen Ma Inc. Aglycosyl anti-CD154 (CD40 ligand) antibodies and uses thereof
CA2529819A1 (en) 2003-06-30 2004-09-23 Domantis Limited Pegylated single domain antibodies
GB0315457D0 (en) 2003-07-01 2003-08-06 Celltech R&D Ltd Biological products
GB0315450D0 (en) 2003-07-01 2003-08-06 Celltech R&D Ltd Biological products
CA2533593A1 (en) 2003-07-26 2005-02-10 Biogen Idec Ma Inc. Altered antibodies having improved antigen-binding affinity
WO2006033702A2 (en) 2004-07-26 2006-03-30 Biogen Idec Ma Inc. Anti-cd154 antibodies
AU2005282700A1 (en) * 2004-09-02 2006-03-16 Genentech, Inc. Heteromultimeric molecules
US7563443B2 (en) 2004-09-17 2009-07-21 Domantis Limited Monovalent anti-CD40L antibody polypeptides and compositions thereof
GB0425534D0 (en) 2004-11-19 2004-12-22 Celltech R&D Ltd Process for obtaining antibodies
GB0520169D0 (en) 2005-10-04 2005-11-09 Celltech R&D Ltd Biological products
CN103214577B (zh) 2007-03-22 2015-09-02 生物基因Ma公司 特异性结合cd154的包括抗体、抗体衍生物和抗体片段在内的结合蛋白及其用途
US7662587B1 (en) 2009-03-05 2010-02-16 E. I. Du Pont De Nemours And Company Gene knockout mutations that increase peptide production
IN2012DN02401A (pt) 2009-09-24 2015-08-21 Ucb Pharma Sa
US8470552B2 (en) 2009-10-12 2013-06-25 Keck Graduate Institute Strategy to reduce lactic acid production and control PH in animal cell culture
KR101968766B1 (ko) 2009-11-05 2019-04-12 제넨테크, 인크. 이종 폴리펩티드의 분비를 위한 방법 및 조성물
GB201000590D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000588D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201000591D0 (en) * 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201001791D0 (en) 2010-02-03 2010-03-24 Ucb Pharma Sa Process for obtaining antibodies
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
BR112013032871B1 (pt) 2011-07-13 2022-09-27 Ucb Pharma S.A. Cepa hospedeira bacteriana que expressa dsbc recombinante e método para produzir anticorpo ou fragmento de ligação de antígeno deste que se liga especificamente a cd154
EP2546267A1 (en) 2011-07-13 2013-01-16 UCB Pharma S.A. Bacterial host strain expressing recombinant DsbC
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product

Also Published As

Publication number Publication date
ME02752B (me) 2018-01-20
US9493559B2 (en) 2016-11-15
PT2523969T (pt) 2017-07-04
WO2011086139A1 (en) 2011-07-21
JP5960608B2 (ja) 2016-08-02
AU2011206587A1 (en) 2012-07-19
CN102712679A (zh) 2012-10-03
RS56159B1 (sr) 2017-11-30
KR101729361B1 (ko) 2017-04-21
US20120288894A1 (en) 2012-11-15
PL2523969T3 (pl) 2017-09-29
CN102712679B (zh) 2015-06-03
HK1176943A1 (en) 2013-08-09
DK2523969T3 (en) 2017-06-12
US20150166652A1 (en) 2015-06-18
ES2627826T3 (es) 2017-07-31
HUE033555T2 (hu) 2017-12-28
AU2017202434A1 (en) 2017-05-04
HK1206785A1 (en) 2016-01-15
SI2523969T1 (sl) 2017-07-31
KR20120115997A (ko) 2012-10-19
LT2523969T (lt) 2017-06-26
AU2017202434B2 (en) 2019-05-16
HRP20170852T1 (hr) 2017-08-25
SG182280A1 (en) 2012-08-30
JP2013516966A (ja) 2013-05-16
CA2786336A1 (en) 2011-07-21
CN104328079A (zh) 2015-02-04
CY1118990T1 (el) 2018-01-10
GB201000591D0 (en) 2010-03-03
EP2523969B1 (en) 2017-03-22
EP2523969A1 (en) 2012-11-21
US8969038B2 (en) 2015-03-03
CA2786336C (en) 2018-09-04

Similar Documents

Publication Publication Date Title
BR112012016808A2 (pt) cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida
BR112012016807A2 (pt) cepa de hospedeiro bacteriano que compreende um gene spr mutante e um gene tsp do tipo selvagem
WO2011103300A3 (en) Improving activity of fe-s cluster requiring proteins
UA108348C2 (uk) Ген bacillus thuringiensis з активністю проти lepidoptera
BR112015002280A2 (pt) produção de gado resistente a vfa por substituição de alelo
AU2009223508A8 (en) Expression of catalase in Trichoderma
MX352023B (es) Variantes de alfa-amilasa con estabilidad mejorada.
BR112013010551A8 (pt) variantes de isopreno sintase para produção aprimorada de isopreno
UA116980C2 (uk) Полінуклеотид, який кодує 5-енолпірувілшикімат-3-фосфатсинтазу, та його використання в наданні рослині стійкості до гліфосату
MX348738B (es) Cepa huesped bacteriana que expresa dsbc recombinante.
BR112012006670B8 (pt) Célula bacteriana gram-negativa recombinante, e, método para produzir uma proteína recombinante
EP2576804A4 (en) PENTOSE FERMENTATION BY RECOMBINANT MICROORGANISM
GB201000588D0 (en) Bacterial host strain
CL2013000032A1 (es) Metodo para modificar el contenido de glucosinolato (gls) en una planta del orden brassicales que comprende modificar la actividad funcional de al menos una proteina transportadora de glucosinolato (gtr); metodo para reducir la actividad de la gtr en una celula de planta; uso de una secuencia que codifica gtr para obtener planta con contenido modiificado de gsl; gen quimérico; proteina aislada; alelo de gtr mutante.
IN2012DE00622A (pt)
EP4067485A3 (en) Lipase variants and polynucleotides encoding same
BR112014010053A2 (pt) variantes de isopreno sintase com solubilidade aprimorada para produção de isopreno
EP3678066B8 (en) Mr-scanner with embedded quantum computer
WO2012092379A3 (en) Cells having disrupted expression of proteins involved in adme and toxicology processes
WO2014170916A3 (en) Uricase mutants
UA101158C2 (ru) ПЕПТИД RumC, КОТОРЫЙ ИМЕЕТ АНТИМИКРОБНУЮ АКТИВНОСТЬ
WO2012082509A3 (en) Biomarkers for enhanced protein production
MX2013013063A (es) Plantas que tienen rasgos mejorados relacionados con el rendimiento y metodo para elaborar las mismas.
Gupta et al. Bioinformatics Analysis of Microbial Genes Encoding Enzymes of Industrial Importance

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]