BR112012016808A2 - cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida - Google Patents

cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida

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Publication number
BR112012016808A2
BR112012016808A2 BR112012016808A BR112012016808A BR112012016808A2 BR 112012016808 A2 BR112012016808 A2 BR 112012016808A2 BR 112012016808 A BR112012016808 A BR 112012016808A BR 112012016808 A BR112012016808 A BR 112012016808A BR 112012016808 A2 BR112012016808 A2 BR 112012016808A2
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BR
Brazil
Prior art keywords
host strain
bacterial host
expressing recombinant
strain expressing
reduced tsp
Prior art date
Application number
BR112012016808A
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English (en)
Inventor
Paul Humphreys David
Ellis Mark
Original Assignee
Ucb Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Pharma Sa filed Critical Ucb Pharma Sa
Publication of BR112012016808A2 publication Critical patent/BR112012016808A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/04—Intramolecular oxidoreductases (5.3) transposing S-S bonds (5.3.4)
    • C12Y503/04001—Protein disulfide-isomerase (5.3.4.1), i.e. disufide bond-forming enzyme
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

cepa de hospedeiro bacteriano que compreende um gene spr mutante e um gene tsp do tipo selvagem. a presente invenção fornece uma célula bacteriana gram negativa recombinante, em que a célula: a. compreende um polinucleotídeo recombinante que codifica dsbc; e b. possui atividade de proteína tsp reduzida comparada à célula do tipo selvagem.
BR112012016808A 2010-01-14 2011-01-13 cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida BR112012016808A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1000591.6A GB201000591D0 (en) 2010-01-14 2010-01-14 Bacterial hoist strain
PCT/EP2011/050416 WO2011086139A1 (en) 2010-01-14 2011-01-13 Bacterial host strain expressing recombinant dsbc and having reduced tsp activity

Publications (1)

Publication Number Publication Date
BR112012016808A2 true BR112012016808A2 (pt) 2017-06-27

Family

ID=42028361

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012016808A BR112012016808A2 (pt) 2010-01-14 2011-01-13 cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida

Country Status (23)

Country Link
US (2) US8969038B2 (pt)
EP (1) EP2523969B1 (pt)
JP (1) JP5960608B2 (pt)
KR (1) KR101729361B1 (pt)
CN (2) CN104328079A (pt)
AU (2) AU2011206587A1 (pt)
BR (1) BR112012016808A2 (pt)
CA (1) CA2786336C (pt)
CY (1) CY1118990T1 (pt)
DK (1) DK2523969T3 (pt)
ES (1) ES2627826T3 (pt)
GB (1) GB201000591D0 (pt)
HK (2) HK1176943A1 (pt)
HR (1) HRP20170852T1 (pt)
HU (1) HUE033555T2 (pt)
LT (1) LT2523969T (pt)
ME (1) ME02752B (pt)
PL (1) PL2523969T3 (pt)
PT (1) PT2523969T (pt)
RS (1) RS56159B1 (pt)
SG (1) SG182280A1 (pt)
SI (1) SI2523969T1 (pt)
WO (1) WO2011086139A1 (pt)

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GB201000591D0 (en) * 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
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GB201001791D0 (en) 2010-02-03 2010-03-24 Ucb Pharma Sa Process for obtaining antibodies
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KR102023786B1 (ko) 2011-07-13 2019-09-20 유씨비 파마, 에스.에이. 재조합 dsbc를 발현하는 세균 숙주 균주
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JP2022525775A (ja) 2019-03-18 2022-05-19 バイオ－ラッド エービーディー セロテック ゲーエムベーハー ＳｐｙＴａｇ含有ペリプラズム融合タンパク質のプロテアーゼＴＳＰ及びＯＭＰＴ分解からの保護

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GB201001791D0 (en) 2010-02-03 2010-03-24 Ucb Pharma Sa Process for obtaining antibodies
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Also Published As

Publication number Publication date
US20120288894A1 (en) 2012-11-15
RS56159B1 (sr) 2017-11-30
LT2523969T (lt) 2017-06-26
US8969038B2 (en) 2015-03-03
PT2523969T (pt) 2017-07-04
CA2786336C (en) 2018-09-04
US9493559B2 (en) 2016-11-15
HK1206785A1 (en) 2016-01-15
KR20120115997A (ko) 2012-10-19
CY1118990T1 (el) 2018-01-10
GB201000591D0 (en) 2010-03-03
CA2786336A1 (en) 2011-07-21
JP2013516966A (ja) 2013-05-16
CN102712679A (zh) 2012-10-03
CN102712679B (zh) 2015-06-03
HUE033555T2 (hu) 2017-12-28
CN104328079A (zh) 2015-02-04
ES2627826T3 (es) 2017-07-31
KR101729361B1 (ko) 2017-04-21
US20150166652A1 (en) 2015-06-18
WO2011086139A1 (en) 2011-07-21
AU2017202434A1 (en) 2017-05-04
ME02752B (me) 2018-01-20
JP5960608B2 (ja) 2016-08-02
PL2523969T3 (pl) 2017-09-29
AU2017202434B2 (en) 2019-05-16
EP2523969A1 (en) 2012-11-21
DK2523969T3 (en) 2017-06-12
HK1176943A1 (en) 2013-08-09
SG182280A1 (en) 2012-08-30
EP2523969B1 (en) 2017-03-22
HRP20170852T1 (hr) 2017-08-25
SI2523969T1 (sl) 2017-07-31
AU2011206587A1 (en) 2012-07-19

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]