BR112012017425A2 - cepa hospedeira bacteriana compreendendo um gene spr mutante e tendo atividade tsp reduzida - Google Patents

cepa hospedeira bacteriana compreendendo um gene spr mutante e tendo atividade tsp reduzida

Info

Publication number
BR112012017425A2
BR112012017425A2 BR112012017425A BR112012017425A BR112012017425A2 BR 112012017425 A2 BR112012017425 A2 BR 112012017425A2 BR 112012017425 A BR112012017425 A BR 112012017425A BR 112012017425 A BR112012017425 A BR 112012017425A BR 112012017425 A2 BR112012017425 A2 BR 112012017425A2
Authority
BR
Brazil
Prior art keywords
host strain
bacterial host
spr gene
reduced tsp
mutant spr
Prior art date
Application number
BR112012017425A
Other languages
English (en)
Inventor
David Paul Humphreys
Mark Ellis
Original Assignee
Ucb Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Pharma Sa filed Critical Ucb Pharma Sa
Publication of BR112012017425A2 publication Critical patent/BR112012017425A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

cepa hospedeira bacteriana compreendendo um gene spr mutante e tendo atividade tsp reduzida. a presente invenção fornece uma célula bacteriana gram negativa recombinante que compreende um gene spr mutante que codifica uma proteína spr tendo uma mutação em um ou mais aminoácidos selecionados de d133, h145, h157, n31, r62, i70, q73, c94,s95, v98, r100, l108, y115, v135, l136, g140, r144 e g147 e em que a célula possui atividade de proteína tsp reduzida comparada com uma célula do tipo selvagem.
BR112012017425A 2010-01-14 2011-01-13 cepa hospedeira bacteriana compreendendo um gene spr mutante e tendo atividade tsp reduzida BR112012017425A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1000587.4A GB201000587D0 (en) 2010-01-14 2010-01-14 Bacterial hoist strain
PCT/EP2011/050413 WO2011086136A1 (en) 2010-01-14 2011-01-13 Bacterial host strain comprising a mutant spr gene and having reduced tsp activity

Publications (1)

Publication Number Publication Date
BR112012017425A2 true BR112012017425A2 (pt) 2015-09-15

Family

ID=42028357

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012017425A BR112012017425A2 (pt) 2010-01-14 2011-01-13 cepa hospedeira bacteriana compreendendo um gene spr mutante e tendo atividade tsp reduzida

Country Status (21)

Country Link
US (2) US8969039B2 (pt)
EP (1) EP2524043B1 (pt)
JP (1) JP5896569B2 (pt)
KR (1) KR101737152B1 (pt)
CN (2) CN105505845A (pt)
AU (1) AU2011206584B2 (pt)
BR (1) BR112012017425A2 (pt)
CA (1) CA2786335C (pt)
CY (1) CY1116480T1 (pt)
DK (1) DK2524043T3 (pt)
ES (1) ES2539931T3 (pt)
GB (1) GB201000587D0 (pt)
HK (1) HK1177760A1 (pt)
HR (1) HRP20150659T1 (pt)
PL (1) PL2524043T3 (pt)
PT (1) PT2524043E (pt)
RS (1) RS54084B1 (pt)
SG (1) SG182301A1 (pt)
SI (1) SI2524043T1 (pt)
SM (1) SMT201500145B (pt)
WO (1) WO2011086136A1 (pt)

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BR112013032871B1 (pt) 2011-07-13 2022-09-27 Ucb Pharma S.A. Cepa hospedeira bacteriana que expressa dsbc recombinante e método para produzir anticorpo ou fragmento de ligação de antígeno deste que se liga especificamente a cd154
GB201208370D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Antibodies
GB201208367D0 (en) * 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
EP3237432B1 (en) 2014-12-22 2023-09-06 UCB Biopharma SRL Protein manufacture
TW201702380A (zh) * 2015-02-27 2017-01-16 再生元醫藥公司 宿主細胞蛋白質修飾
BR112018015259A2 (pt) 2016-01-27 2018-12-18 Medimmune Llc métodos para preparação de anticorpos com um padrão de glicosilação definido
EP3942079A1 (en) 2019-03-18 2022-01-26 Bio-Rad ABD Serotec GmbH Protection of spytag-containing periplasmic fusion proteins from protease tsp and ompt degradation
KR20220031670A (ko) * 2019-08-05 2022-03-11 와커 헤미 아게 발효 방법에서 재조합 단백질 방출용 박테리아 균주
CN113462666B (zh) * 2021-08-18 2023-04-07 杭州文德阶生物科技有限公司 羰基还原酶突变体及其应用
SE545714C2 (en) * 2021-09-24 2023-12-19 Xbrane Biopharma Ab Dna contructs for producing a pelb signal peptide
SE545694C2 (en) * 2021-09-24 2023-12-05 Xbrane Biopharma Ab Dna construct comprising nucleotide sequences encoding amino acid sequences of certolizumab and pelb signal peptides

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Also Published As

Publication number Publication date
KR20130030247A (ko) 2013-03-26
SMT201500145B (it) 2015-09-07
CN102762732A (zh) 2012-10-31
CA2786335A1 (en) 2011-07-21
KR101737152B1 (ko) 2017-05-17
JP2013516964A (ja) 2013-05-16
PT2524043E (pt) 2015-07-22
SG182301A1 (en) 2012-08-30
AU2011206584B2 (en) 2016-06-09
US20120295309A1 (en) 2012-11-22
EP2524043B1 (en) 2015-03-18
ES2539931T3 (es) 2015-07-07
PL2524043T3 (pl) 2016-08-31
US20150132828A1 (en) 2015-05-14
SI2524043T1 (sl) 2015-07-31
HRP20150659T1 (hr) 2015-07-31
HK1177760A1 (zh) 2013-08-30
JP5896569B2 (ja) 2016-03-30
WO2011086136A1 (en) 2011-07-21
RS54084B1 (en) 2015-10-30
US8969039B2 (en) 2015-03-03
GB201000587D0 (en) 2010-03-03
US9587227B2 (en) 2017-03-07
CY1116480T1 (el) 2018-03-07
DK2524043T3 (da) 2015-06-22
CN105505845A (zh) 2016-04-20
EP2524043A1 (en) 2012-11-21
CA2786335C (en) 2017-10-10
CN102762732B (zh) 2016-03-09
AU2011206584A1 (en) 2012-07-26

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
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