DK2524043T3 - Bakteriel værtsstamme omfattende et muteret SPR gen med nedsat TSP aktivitet - Google Patents

Bakteriel værtsstamme omfattende et muteret SPR gen med nedsat TSP aktivitet

Info

Publication number
DK2524043T3
DK2524043T3 DK11701216.1T DK11701216T DK2524043T3 DK 2524043 T3 DK2524043 T3 DK 2524043T3 DK 11701216 T DK11701216 T DK 11701216T DK 2524043 T3 DK2524043 T3 DK 2524043T3
Authority
DK
Denmark
Prior art keywords
bacterial host
host strain
spr gene
mutated spr
tsp activity
Prior art date
Application number
DK11701216.1T
Other languages
English (en)
Inventor
Mark Ellis
David Paul Humphreys
Original Assignee
Ucb Biopharma Sprl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Biopharma Sprl filed Critical Ucb Biopharma Sprl
Application granted granted Critical
Publication of DK2524043T3 publication Critical patent/DK2524043T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK11701216.1T 2010-01-14 2011-01-13 Bakteriel værtsstamme omfattende et muteret SPR gen med nedsat TSP aktivitet DK2524043T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1000587.4A GB201000587D0 (en) 2010-01-14 2010-01-14 Bacterial hoist strain
PCT/EP2011/050413 WO2011086136A1 (en) 2010-01-14 2011-01-13 Bacterial host strain comprising a mutant spr gene and having reduced tsp activity

Publications (1)

Publication Number Publication Date
DK2524043T3 true DK2524043T3 (da) 2015-06-22

Family

ID=42028357

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11701216.1T DK2524043T3 (da) 2010-01-14 2011-01-13 Bakteriel værtsstamme omfattende et muteret SPR gen med nedsat TSP aktivitet

Country Status (21)

Country Link
US (2) US8969039B2 (da)
EP (1) EP2524043B1 (da)
JP (1) JP5896569B2 (da)
KR (1) KR101737152B1 (da)
CN (2) CN102762732B (da)
AU (1) AU2011206584B2 (da)
BR (1) BR112012017425A2 (da)
CA (1) CA2786335C (da)
CY (1) CY1116480T1 (da)
DK (1) DK2524043T3 (da)
ES (1) ES2539931T3 (da)
GB (1) GB201000587D0 (da)
HK (1) HK1177760A1 (da)
HR (1) HRP20150659T1 (da)
PL (1) PL2524043T3 (da)
PT (1) PT2524043E (da)
RS (1) RS54084B1 (da)
SG (1) SG182301A1 (da)
SI (1) SI2524043T1 (da)
SM (1) SMT201500145B (da)
WO (1) WO2011086136A1 (da)

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GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
CN103649124B (zh) * 2011-07-13 2016-04-13 Ucb医药有限公司 表达重组dsbc的细菌宿主菌株
GB201208370D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Antibodies
GB201208367D0 (en) * 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
SI3237432T1 (sl) 2014-12-22 2023-12-29 UCB Biopharma SRL Izdelava proteinov
TW201702380A (zh) * 2015-02-27 2017-01-16 再生元醫藥公司 宿主細胞蛋白質修飾
EP3407914A4 (en) 2016-01-27 2019-08-07 MedImmune, LLC METHOD FOR OBTAINING ANTIBODIES WITH DEFINED GLYCOSILATION PATTERN
JP2022525775A (ja) 2019-03-18 2022-05-19 バイオ－ラッド エービーディー セロテック ゲーエムベーハー ＳｐｙＴａｇ含有ペリプラズム融合タンパク質のプロテアーゼＴＳＰ及びＯＭＰＴ分解からの保護
CN114829578A (zh) * 2019-08-05 2022-07-29 瓦克化学股份公司 用于在发酵方法中释放重组蛋白的细菌菌株
CN113462666B (zh) * 2021-08-18 2023-04-07 杭州文德阶生物科技有限公司 羰基还原酶突变体及其应用
SE545694C2 (en) * 2021-09-24 2023-12-05 Xbrane Biopharma Ab Dna construct comprising nucleotide sequences encoding amino acid sequences of certolizumab and pelb signal peptides
SE545714C2 (en) * 2021-09-24 2023-12-19 Xbrane Biopharma Ab Dna contructs for producing a pelb signal peptide

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Also Published As

Publication number Publication date
SI2524043T1 (sl) 2015-07-31
KR20130030247A (ko) 2013-03-26
HRP20150659T1 (hr) 2015-07-31
US20120295309A1 (en) 2012-11-22
GB201000587D0 (en) 2010-03-03
CN105505845A (zh) 2016-04-20
CY1116480T1 (el) 2018-03-07
US9587227B2 (en) 2017-03-07
HK1177760A1 (zh) 2013-08-30
JP5896569B2 (ja) 2016-03-30
BR112012017425A2 (pt) 2015-09-15
CN102762732A (zh) 2012-10-31
AU2011206584B2 (en) 2016-06-09
AU2011206584A1 (en) 2012-07-26
WO2011086136A1 (en) 2011-07-21
US20150132828A1 (en) 2015-05-14
PL2524043T3 (pl) 2016-08-31
US8969039B2 (en) 2015-03-03
KR101737152B1 (ko) 2017-05-17
PT2524043E (pt) 2015-07-22
SG182301A1 (en) 2012-08-30
ES2539931T3 (es) 2015-07-07
CA2786335A1 (en) 2011-07-21
RS54084B1 (en) 2015-10-30
JP2013516964A (ja) 2013-05-16
CA2786335C (en) 2017-10-10
EP2524043A1 (en) 2012-11-21
EP2524043B1 (en) 2015-03-18
CN102762732B (zh) 2016-03-09
SMT201500145B (it) 2015-09-07

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