BR112012015596B1 - Processo de cultura de células aderentes - Google Patents

Processo de cultura de células aderentes Download PDF

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Publication number
BR112012015596B1
BR112012015596B1 BR112012015596-2A BR112012015596A BR112012015596B1 BR 112012015596 B1 BR112012015596 B1 BR 112012015596B1 BR 112012015596 A BR112012015596 A BR 112012015596A BR 112012015596 B1 BR112012015596 B1 BR 112012015596B1
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Brazil
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cell
cells
microcarriers
culture
culture medium
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BR112012015596-2A
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English (en)
Portuguese (pt)
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BR112012015596A2 (pt
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Eric Calvosa
Nicolas Seve
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Sanofi Pasteur
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Priority claimed from FR1051754A external-priority patent/FR2957355B1/fr
Application filed by Sanofi Pasteur filed Critical Sanofi Pasteur
Publication of BR112012015596A2 publication Critical patent/BR112012015596A2/pt
Publication of BR112012015596B1 publication Critical patent/BR112012015596B1/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0056Xeno-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/14Antivirals for RNA viruses
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • C12N5/0075General culture methods using substrates using microcarriers
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
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    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92Medium free of human- or animal-derived components
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    • C12N2710/00011Details
    • C12N2710/16011Herpesviridae
    • C12N2710/16711Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
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    • C12N2760/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011Details
    • C12N2760/20011Rhabdoviridae
    • C12N2760/20111Lyssavirus, e.g. rabies virus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2760/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011Details
    • C12N2760/20011Rhabdoviridae
    • C12N2760/20111Lyssavirus, e.g. rabies virus
    • C12N2760/20151Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
BR112012015596-2A 2009-12-23 2010-12-21 Processo de cultura de células aderentes BR112012015596B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
FR0959472 2009-12-23
FR0959472 2009-12-23
FR1051754 2010-03-11
FR1051754A FR2957355B1 (fr) 2010-03-11 2010-03-11 Procede de culture de cellules adherentes a des microporteurs
PCT/FR2010/052850 WO2011077035A1 (fr) 2009-12-23 2010-12-21 Procede de culture de cellules adherentes

Publications (2)

Publication Number Publication Date
BR112012015596A2 BR112012015596A2 (pt) 2015-11-03
BR112012015596B1 true BR112012015596B1 (pt) 2021-06-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012015596-2A BR112012015596B1 (pt) 2009-12-23 2010-12-21 Processo de cultura de células aderentes

Country Status (14)

Country Link
US (1) US9145543B2 (https=)
EP (1) EP2516623B1 (https=)
JP (1) JP5894929B2 (https=)
KR (1) KR101835100B1 (https=)
CN (2) CN102782121A (https=)
AR (1) AR079710A1 (https=)
AU (1) AU2010334723C1 (https=)
BR (1) BR112012015596B1 (https=)
CA (1) CA2784741A1 (https=)
HK (1) HK1243731A1 (https=)
IL (1) IL220167B (https=)
MX (1) MX2012007117A (https=)
RU (1) RU2612154C2 (https=)
WO (1) WO2011077035A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140271633A1 (en) * 2013-03-14 2014-09-18 Abbvie Inc. Mammalian cell culture performance through surfactant supplementation of feed media
CN103289949A (zh) * 2013-06-28 2013-09-11 四川省华派生物制药有限公司 一种从克氏瓶直接到生物反应器的细胞培养方法
BR112016021739B1 (pt) * 2014-03-25 2021-11-03 Genentech, Inc Método para preparar um poloxâmero para uso em um meio de cultura de célula
PT3152317T (pt) * 2014-06-04 2019-04-18 Amgen Inc Métodos de colheita de culturas de células de mamíferos
JP7364334B2 (ja) 2016-06-24 2023-10-18 ロンザ リミテッド 直径可変型バイオリアクタ
CN106754672A (zh) * 2016-11-30 2017-05-31 广州赛莱拉干细胞科技股份有限公司 一种贴壁细胞的培养方法
WO2019240962A1 (en) * 2018-06-14 2019-12-19 Abraham J And Phyllis Katz Cord Blood Foundation Isolation of mesenchymal stromal cells from umbilical cord blood
CN115485363A (zh) * 2020-03-13 2022-12-16 昭和电工材料株式会社 细胞悬液的制造方法和黏附细胞的制造方法
EP4166644A1 (en) * 2020-06-12 2023-04-19 Biotech Foods S.L. Process and device for production of cultured meat
WO2022197878A1 (en) * 2021-03-19 2022-09-22 Merck Sharp & Dohme Llc A closed-system upstream manufacturing process for dengue virus production
CN116036257B (zh) * 2022-12-29 2024-11-15 瑞普(保定)生物药业有限公司 一种猪瘟活疫苗的制备方法
WO2025125314A1 (fr) 2023-12-11 2025-06-19 Commissariat A L'energie Atomique Et Aux Energies Alternatives Dispositif et procédé de culture cellulaire

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4664912A (en) 1984-10-01 1987-05-12 Wiktor Tadeusz J Process for the large scale production of rabies vaccine
FR2635008B1 (fr) 1988-08-05 1990-11-16 Sanofi Sa Agent activateur de la productivite specifique des cellules animales recombinantes a base de polyvinylpyrrolidone et milieu de culture defini le contenant
DK52393D0 (https=) 1993-05-05 1993-05-05 Novo Nordisk As
US5714375A (en) * 1995-06-05 1998-02-03 Nobl Laboratories, Inc. Ileal symbiont intracellularis propagation in suspended host cells
US6020182A (en) * 1996-07-12 2000-02-01 Connaught Laboratories Limited Subunit respiratory syncytial virus vaccine preparation
DE69720024D1 (de) * 1996-07-12 2003-04-24 Aventis Pasteur Immunisierungsverfahren in zwei schritten gegen pyramyxoviridae-viren unter verwendung abgeschwächter viraler stämme und preparation von proteinuntereinheiten
US6168944B1 (en) * 1997-01-31 2001-01-02 Schering Corporation Methods for cultivating cells and propagating viruses
ATE356197T1 (de) 1997-12-24 2007-03-15 Duphar Int Res Herstellung von zellen für die produktion von biologischen produkten
FR2801900B1 (fr) 1999-12-03 2004-02-27 Pasteur Merieux Serums Vacc Methode de production de cellules animales adherentes
US6670184B2 (en) * 2000-06-23 2003-12-30 Basf Ag Method for producing a lipid emulsion for use in insect cell culture
US6951752B2 (en) * 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen
US6855535B2 (en) * 2001-12-10 2005-02-15 Baxter Healthcare S.A. Method of large scale production of Hepatitis A virus
DE102004043256B4 (de) * 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
CN101265464B (zh) * 2008-05-13 2010-06-16 中国人民解放军第二军医大学 一种猪骨髓内皮祖细胞的分离和培养方法

Also Published As

Publication number Publication date
JP2013515473A (ja) 2013-05-09
HK1243731A1 (zh) 2018-07-20
CN107460154A (zh) 2017-12-12
CN102782121A (zh) 2012-11-14
US20110151506A1 (en) 2011-06-23
BR112012015596A2 (pt) 2015-11-03
KR20120102788A (ko) 2012-09-18
RU2612154C2 (ru) 2017-03-02
WO2011077035A1 (fr) 2011-06-30
MX2012007117A (es) 2012-07-23
KR101835100B1 (ko) 2018-03-08
EP2516623B1 (fr) 2016-10-05
CA2784741A1 (fr) 2011-06-30
AR079710A1 (es) 2012-02-15
AU2010334723B2 (en) 2015-02-05
US9145543B2 (en) 2015-09-29
IL220167B (en) 2018-01-31
IL220167A0 (en) 2012-07-31
AU2010334723C1 (en) 2015-05-28
AU2010334723A1 (en) 2012-07-12
JP5894929B2 (ja) 2016-03-30
RU2012130928A (ru) 2014-01-27
EP2516623A1 (fr) 2012-10-31

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B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B25G Requested change of headquarter approved

Owner name: SANOFI PASTEUR (FR)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07B Technical examination (opinion): publication cancelled [chapter 7.2 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 7.4 NA RPI NO 2475 DE 12/06/2018 POR TER SIDO INDEVIDA.

B06T Formal requirements before examination [chapter 6.20 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 21/12/2010, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF