JP2013515473A5 - - Google Patents
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- Publication number
- JP2013515473A5 JP2013515473A5 JP2012545386A JP2012545386A JP2013515473A5 JP 2013515473 A5 JP2013515473 A5 JP 2013515473A5 JP 2012545386 A JP2012545386 A JP 2012545386A JP 2012545386 A JP2012545386 A JP 2012545386A JP 2013515473 A5 JP2013515473 A5 JP 2013515473A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- cells
- culture vessel
- microcarriers
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000004027 cell Anatomy 0.000 claims description 215
- 238000000034 method Methods 0.000 claims description 54
- 239000001963 growth medium Substances 0.000 claims description 41
- 230000001464 adherent effect Effects 0.000 claims description 36
- 239000003124 biologic agent Substances 0.000 claims description 35
- 238000004519 manufacturing process Methods 0.000 claims description 29
- 230000002255 enzymatic effect Effects 0.000 claims description 13
- 239000002609 medium Substances 0.000 claims description 8
- 208000015181 infectious disease Diseases 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 239000012678 infectious agent Substances 0.000 claims description 6
- 210000002966 serum Anatomy 0.000 claims description 4
- 102000035195 Peptidases Human genes 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 241000711798 Rabies lyssavirus Species 0.000 claims description 3
- 102000004142 Trypsin Human genes 0.000 claims description 3
- 108090000631 Trypsin Proteins 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 230000002458 infectious effect Effects 0.000 claims description 3
- 239000012588 trypsin Substances 0.000 claims description 3
- 210000003501 vero cell Anatomy 0.000 claims description 3
- 230000001120 cytoprotective effect Effects 0.000 claims 2
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 claims 1
- 244000052769 pathogen Species 0.000 claims 1
- 230000001717 pathogenic effect Effects 0.000 claims 1
- 229960000502 poloxamer Drugs 0.000 claims 1
- 229920001983 poloxamer Polymers 0.000 claims 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 claims 1
- 238000010257 thawing Methods 0.000 claims 1
- 241000700605 Viruses Species 0.000 description 7
- 239000011325 microbead Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 230000002101 lytic effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000991587 Enterovirus C Species 0.000 description 1
- 206010037742 Rabies Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000013587 production medium Substances 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0959472 | 2009-12-23 | ||
| FR0959472 | 2009-12-23 | ||
| FR1051754 | 2010-03-11 | ||
| FR1051754A FR2957355B1 (fr) | 2010-03-11 | 2010-03-11 | Procede de culture de cellules adherentes a des microporteurs |
| PCT/FR2010/052850 WO2011077035A1 (fr) | 2009-12-23 | 2010-12-21 | Procede de culture de cellules adherentes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013515473A JP2013515473A (ja) | 2013-05-09 |
| JP2013515473A5 true JP2013515473A5 (https=) | 2014-02-13 |
| JP5894929B2 JP5894929B2 (ja) | 2016-03-30 |
Family
ID=43709027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012545386A Active JP5894929B2 (ja) | 2009-12-23 | 2010-12-21 | 接着細胞の培養方法 |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US9145543B2 (https=) |
| EP (1) | EP2516623B1 (https=) |
| JP (1) | JP5894929B2 (https=) |
| KR (1) | KR101835100B1 (https=) |
| CN (2) | CN102782121A (https=) |
| AR (1) | AR079710A1 (https=) |
| AU (1) | AU2010334723C1 (https=) |
| BR (1) | BR112012015596B1 (https=) |
| CA (1) | CA2784741A1 (https=) |
| HK (1) | HK1243731A1 (https=) |
| IL (1) | IL220167B (https=) |
| MX (1) | MX2012007117A (https=) |
| RU (1) | RU2612154C2 (https=) |
| WO (1) | WO2011077035A1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140271633A1 (en) * | 2013-03-14 | 2014-09-18 | Abbvie Inc. | Mammalian cell culture performance through surfactant supplementation of feed media |
| CN103289949A (zh) * | 2013-06-28 | 2013-09-11 | 四川省华派生物制药有限公司 | 一种从克氏瓶直接到生物反应器的细胞培养方法 |
| BR112016021739B1 (pt) * | 2014-03-25 | 2021-11-03 | Genentech, Inc | Método para preparar um poloxâmero para uso em um meio de cultura de célula |
| PT3152317T (pt) * | 2014-06-04 | 2019-04-18 | Amgen Inc | Métodos de colheita de culturas de células de mamíferos |
| JP7364334B2 (ja) | 2016-06-24 | 2023-10-18 | ロンザ リミテッド | 直径可変型バイオリアクタ |
| CN106754672A (zh) * | 2016-11-30 | 2017-05-31 | 广州赛莱拉干细胞科技股份有限公司 | 一种贴壁细胞的培养方法 |
| WO2019240962A1 (en) * | 2018-06-14 | 2019-12-19 | Abraham J And Phyllis Katz Cord Blood Foundation | Isolation of mesenchymal stromal cells from umbilical cord blood |
| CN115485363A (zh) * | 2020-03-13 | 2022-12-16 | 昭和电工材料株式会社 | 细胞悬液的制造方法和黏附细胞的制造方法 |
| EP4166644A1 (en) * | 2020-06-12 | 2023-04-19 | Biotech Foods S.L. | Process and device for production of cultured meat |
| WO2022197878A1 (en) * | 2021-03-19 | 2022-09-22 | Merck Sharp & Dohme Llc | A closed-system upstream manufacturing process for dengue virus production |
| CN116036257B (zh) * | 2022-12-29 | 2024-11-15 | 瑞普（保定）生物药业有限公司 | 一种猪瘟活疫苗的制备方法 |
| WO2025125314A1 (fr) | 2023-12-11 | 2025-06-19 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Dispositif et procédé de culture cellulaire |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4664912A (en) | 1984-10-01 | 1987-05-12 | Wiktor Tadeusz J | Process for the large scale production of rabies vaccine |
| FR2635008B1 (fr) | 1988-08-05 | 1990-11-16 | Sanofi Sa | Agent activateur de la productivite specifique des cellules animales recombinantes a base de polyvinylpyrrolidone et milieu de culture defini le contenant |
| DK52393D0 (https=) | 1993-05-05 | 1993-05-05 | Novo Nordisk As | |
| US5714375A (en) * | 1995-06-05 | 1998-02-03 | Nobl Laboratories, Inc. | Ileal symbiont intracellularis propagation in suspended host cells |
| US6020182A (en) * | 1996-07-12 | 2000-02-01 | Connaught Laboratories Limited | Subunit respiratory syncytial virus vaccine preparation |
| DE69720024D1 (de) * | 1996-07-12 | 2003-04-24 | Aventis Pasteur | Immunisierungsverfahren in zwei schritten gegen pyramyxoviridae-viren unter verwendung abgeschwächter viraler stämme und preparation von proteinuntereinheiten |
| US6168944B1 (en) * | 1997-01-31 | 2001-01-02 | Schering Corporation | Methods for cultivating cells and propagating viruses |
| ATE356197T1 (de) | 1997-12-24 | 2007-03-15 | Duphar Int Res | Herstellung von zellen für die produktion von biologischen produkten |
| FR2801900B1 (fr) | 1999-12-03 | 2004-02-27 | Pasteur Merieux Serums Vacc | Methode de production de cellules animales adherentes |
| US6670184B2 (en) * | 2000-06-23 | 2003-12-30 | Basf Ag | Method for producing a lipid emulsion for use in insect cell culture |
| US6951752B2 (en) * | 2001-12-10 | 2005-10-04 | Bexter Healthcare S.A. | Method for large scale production of virus antigen |
| US6855535B2 (en) * | 2001-12-10 | 2005-02-15 | Baxter Healthcare S.A. | Method of large scale production of Hepatitis A virus |
| DE102004043256B4 (de) * | 2004-09-07 | 2013-09-19 | Rheinische Friedrich-Wilhelms-Universität Bonn | Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension |
| CN101265464B (zh) * | 2008-05-13 | 2010-06-16 | 中国人民解放军第二军医大学 | 一种猪骨髓内皮祖细胞的分离和培养方法 |
-
2010
- 2010-12-21 RU RU2012130928A patent/RU2612154C2/ru active
- 2010-12-21 KR KR1020127018857A patent/KR101835100B1/ko active Active
- 2010-12-21 BR BR112012015596-2A patent/BR112012015596B1/pt active IP Right Grant
- 2010-12-21 JP JP2012545386A patent/JP5894929B2/ja active Active
- 2010-12-21 EP EP10810863.0A patent/EP2516623B1/fr active Active
- 2010-12-21 CN CN2010800645251A patent/CN102782121A/zh active Pending
- 2010-12-21 CA CA2784741A patent/CA2784741A1/fr not_active Abandoned
- 2010-12-21 MX MX2012007117A patent/MX2012007117A/es active IP Right Grant
- 2010-12-21 AU AU2010334723A patent/AU2010334723C1/en active Active
- 2010-12-21 WO PCT/FR2010/052850 patent/WO2011077035A1/fr not_active Ceased
- 2010-12-21 CN CN201710197964.3A patent/CN107460154A/zh active Pending
- 2010-12-22 AR ARP100104895 patent/AR079710A1/es active IP Right Grant
- 2010-12-22 US US12/976,679 patent/US9145543B2/en active Active
-
2012
- 2012-06-04 IL IL220167A patent/IL220167B/en active IP Right Grant
-
2018
- 2018-03-05 HK HK18103103.8A patent/HK1243731A1/zh unknown
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