AR079710A1 - Proceso para el cultivo de celulas adherentes - Google Patents
Proceso para el cultivo de celulas adherentesInfo
- Publication number
- AR079710A1 AR079710A1 ARP100104895A AR079710A1 AR 079710 A1 AR079710 A1 AR 079710A1 AR P100104895 A ARP100104895 A AR P100104895A AR 079710 A1 AR079710 A1 AR 079710A1
- Authority
- AR
- Argentina
- Prior art keywords
- microcarriers
- cells
- cell
- cell population
- detach
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000004115 adherent culture Methods 0.000 title 1
- 230000001464 adherent effect Effects 0.000 abstract 2
- 230000002255 enzymatic effect Effects 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 241000711798 Rabies lyssavirus Species 0.000 abstract 1
- 208000036142 Viral infection Diseases 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 239000003124 biologic agent Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 239000012678 infectious agent Substances 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
- 230000009385 viral infection Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0056—Xeno-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C12N2500/92—Medium free of human- or animal-derived components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/99—Serum-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/20011—Rhabdoviridae
- C12N2760/20111—Lyssavirus, e.g. rabies virus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/20011—Rhabdoviridae
- C12N2760/20111—Lyssavirus, e.g. rabies virus
- C12N2760/20151—Methods of production or purification of viral material
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Communicable Diseases (AREA)
- Public Health (AREA)
- Oncology (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Un proceso para la produccion de células adherentes, que comprende: a) la introduccion de una suspension de células adherentes en un recipiente de cultivo que contiene microportadores en un medio de cultivo; b) la amplificacion de las células, efectuando varios pases celulares sucesivos en este mismo recipiente de cultivo, donde cada pase de células sucesivo se lleva a cabo: i) mediante el uso, cada vez, de la totalidad o parte de la poblacion de células producida durante el pase celular previo, y que ha sido sometida al tratamiento enzimático a fin de desprender las células de los microportadores; y ii) mediante la introduccion, cada vez, de una cantidad creciente de microportadores en el medio de cultivo; y c) la cosecha de la poblacion de células producida durante el ultimo pase de células, luego de haber sometido, en forma opcional, dicha poblacion de células al tratamiento enzimático, a fin de desprender las células de los microportadores. Además se refiere a la implementacion de este proceso para la produccion de agentes biologicos, que sirven, en particular, para la preparacion de vacunas o fármacos. Reivindicacion 4: El proceso como se reivindica en la reivindicacion 3, donde el agente infeccioso es el virus de la rabia, y donde el medio de infeccion es un medio de infeccion viral libre de cualquier producto de origen animal.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0959472 | 2009-12-23 | ||
| FR1051754A FR2957355B1 (fr) | 2010-03-11 | 2010-03-11 | Procede de culture de cellules adherentes a des microporteurs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR079710A1 true AR079710A1 (es) | 2012-02-15 |
Family
ID=43709027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP100104895 AR079710A1 (es) | 2009-12-23 | 2010-12-22 | Proceso para el cultivo de celulas adherentes |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US9145543B2 (es) |
| EP (1) | EP2516623B1 (es) |
| JP (1) | JP5894929B2 (es) |
| KR (1) | KR101835100B1 (es) |
| CN (2) | CN102782121A (es) |
| AR (1) | AR079710A1 (es) |
| AU (1) | AU2010334723C1 (es) |
| BR (1) | BR112012015596B1 (es) |
| CA (1) | CA2784741A1 (es) |
| HK (1) | HK1243731A1 (es) |
| IL (1) | IL220167B (es) |
| MX (1) | MX2012007117A (es) |
| RU (1) | RU2612154C2 (es) |
| WO (1) | WO2011077035A1 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140271633A1 (en) * | 2013-03-14 | 2014-09-18 | Abbvie Inc. | Mammalian cell culture performance through surfactant supplementation of feed media |
| CN103289949A (zh) * | 2013-06-28 | 2013-09-11 | 四川省华派生物制药有限公司 | 一种从克氏瓶直接到生物反应器的细胞培养方法 |
| EP3122799B8 (en) * | 2014-03-25 | 2020-03-11 | F.Hoffmann-La Roche Ag | Methods of preparing a poloxamer for use in cell culture medium |
| ES2909630T3 (es) | 2014-06-04 | 2022-05-09 | Amgen Inc | Métodos de recolección en cultivos de células de mamífero |
| TWI808057B (zh) * | 2016-06-24 | 2023-07-11 | 瑞士商隆沙有限公司 | 可變直徑生物感測器容器及系統，在接種階段列及生產感測器中使用減少量之感測器生產醱酵產物之方法，及生物生產設施 |
| CN106754672A (zh) * | 2016-11-30 | 2017-05-31 | 广州赛莱拉干细胞科技股份有限公司 | 一种贴壁细胞的培养方法 |
| CA3103570A1 (en) * | 2018-06-14 | 2019-12-19 | Abraham J And Phyllis Katz Cord Blood Foundation | Isolation of mesenchymal stromal cells from umbilical cord blood |
| JPWO2021181819A1 (es) * | 2020-03-13 | 2021-09-16 | ||
| CN116036257A (zh) * | 2022-12-29 | 2023-05-02 | 瑞普（保定）生物药业有限公司 | 一种猪瘟活疫苗的制备方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4664912A (en) | 1984-10-01 | 1987-05-12 | Wiktor Tadeusz J | Process for the large scale production of rabies vaccine |
| FR2635008B1 (fr) | 1988-08-05 | 1990-11-16 | Sanofi Sa | Agent activateur de la productivite specifique des cellules animales recombinantes a base de polyvinylpyrrolidone et milieu de culture defini le contenant |
| DK52393D0 (es) | 1993-05-05 | 1993-05-05 | Novo Nordisk As | |
| US5714375A (en) * | 1995-06-05 | 1998-02-03 | Nobl Laboratories, Inc. | Ileal symbiont intracellularis propagation in suspended host cells |
| US6020182A (en) * | 1996-07-12 | 2000-02-01 | Connaught Laboratories Limited | Subunit respiratory syncytial virus vaccine preparation |
| EP0936921B1 (en) * | 1996-07-12 | 2003-03-19 | Aventis Pasteur Limited | Two-step immunization procedure against the pyramyxoviridae family of viruses using attenuated viral strains and subunit protein preparation |
| US6168944B1 (en) * | 1997-01-31 | 2001-01-02 | Schering Corporation | Methods for cultivating cells and propagating viruses |
| PT1801201E (pt) | 1997-12-24 | 2011-07-05 | Abbott Biologicals Bv | Preparação de células para a produção de material biológico |
| FR2801900B1 (fr) | 1999-12-03 | 2004-02-27 | Pasteur Merieux Serums Vacc | Methode de production de cellules animales adherentes |
| US6670184B2 (en) * | 2000-06-23 | 2003-12-30 | Basf Ag | Method for producing a lipid emulsion for use in insect cell culture |
| US6855535B2 (en) * | 2001-12-10 | 2005-02-15 | Baxter Healthcare S.A. | Method of large scale production of Hepatitis A virus |
| US6951752B2 (en) * | 2001-12-10 | 2005-10-04 | Bexter Healthcare S.A. | Method for large scale production of virus antigen |
| DE102004043256B4 (de) * | 2004-09-07 | 2013-09-19 | Rheinische Friedrich-Wilhelms-Universität Bonn | Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension |
| CN101265464B (zh) * | 2008-05-13 | 2010-06-16 | 中国人民解放军第二军医大学 | 一种猪骨髓内皮祖细胞的分离和培养方法 |
-
2010
- 2010-12-21 BR BR112012015596-2A patent/BR112012015596B1/pt active IP Right Grant
- 2010-12-21 CN CN2010800645251A patent/CN102782121A/zh active Pending
- 2010-12-21 WO PCT/FR2010/052850 patent/WO2011077035A1/fr active Application Filing
- 2010-12-21 JP JP2012545386A patent/JP5894929B2/ja active Active
- 2010-12-21 CN CN201710197964.3A patent/CN107460154A/zh active Pending
- 2010-12-21 CA CA2784741A patent/CA2784741A1/fr not_active Abandoned
- 2010-12-21 AU AU2010334723A patent/AU2010334723C1/en active Active
- 2010-12-21 EP EP10810863.0A patent/EP2516623B1/fr active Active
- 2010-12-21 RU RU2012130928A patent/RU2612154C2/ru active
- 2010-12-21 MX MX2012007117A patent/MX2012007117A/es active IP Right Grant
- 2010-12-21 KR KR1020127018857A patent/KR101835100B1/ko active IP Right Grant
- 2010-12-22 US US12/976,679 patent/US9145543B2/en active Active
- 2010-12-22 AR ARP100104895 patent/AR079710A1/es active IP Right Grant
-
2012
- 2012-06-04 IL IL220167A patent/IL220167B/en active IP Right Grant
-
2018
- 2018-03-05 HK HK18103103.8A patent/HK1243731A1/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2516623B1 (fr) | 2016-10-05 |
| AU2010334723C1 (en) | 2015-05-28 |
| HK1243731A1 (zh) | 2018-07-20 |
| BR112012015596B1 (pt) | 2021-06-01 |
| US9145543B2 (en) | 2015-09-29 |
| CN102782121A (zh) | 2012-11-14 |
| KR20120102788A (ko) | 2012-09-18 |
| KR101835100B1 (ko) | 2018-03-08 |
| BR112012015596A2 (pt) | 2015-11-03 |
| US20110151506A1 (en) | 2011-06-23 |
| JP2013515473A (ja) | 2013-05-09 |
| RU2012130928A (ru) | 2014-01-27 |
| CA2784741A1 (fr) | 2011-06-30 |
| CN107460154A (zh) | 2017-12-12 |
| IL220167B (en) | 2018-01-31 |
| AU2010334723B2 (en) | 2015-02-05 |
| MX2012007117A (es) | 2012-07-23 |
| RU2612154C2 (ru) | 2017-03-02 |
| EP2516623A1 (fr) | 2012-10-31 |
| IL220167A0 (en) | 2012-07-31 |
| JP5894929B2 (ja) | 2016-03-30 |
| AU2010334723A1 (en) | 2012-07-12 |
| WO2011077035A1 (fr) | 2011-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AR079710A1 (es) | Proceso para el cultivo de celulas adherentes | |
| EA201270173A1 (ru) | Получение полиовируса с высокими титрами для получения вакцины | |
| EA200801221A1 (ru) | Вирусные вакцины, полученные из клеток, с низкими уровнями остаточной клеточной днк | |
| JP2017513477A5 (es) | ||
| JP2019520844A5 (es) | ||
| WO2007002008A3 (en) | Methods and compositions for expressing negative-sense viral rna in canine cells | |
| WO2013039861A3 (en) | Engineered nucleic acids and methods of use thereof | |
| MY151119A (en) | Duck embryonic derived stem cell lines for the production of viral vaccines | |
| DE60139026D1 (de) | Lebender influenza impfstoff und verfahren zu seiner herstellung | |
| EP2471551A3 (en) | Decreasing potential iatrogenic risks associated with influenza vaccines | |
| MXPA05009096A (es) | Metodo de cultivo celular libre de animal. | |
| ATE469972T1 (de) | Multiplasmid-system zur erzeugung des grippevirus | |
| EP3418386A3 (en) | Compositions and methods for inhibiting gene expression of hepatitis b virus | |
| WO2011082388A3 (en) | Infectious dna vaccines against chikungunya virus | |
| WO2007124327A3 (en) | Methods and compositions for expressing negative-sense viral rna in canine cells | |
| MX2014010482A (es) | Metodo para la preparacion de vacunas de celulas dendriticas. | |
| RU2017104621A (ru) | Способ совместного кондиционирования и хемоселекции за один цикл | |
| MX2021009554A (es) | Produccion de virus en cultivos celulares. | |
| AR094389A1 (es) | USO DE INHIBIDORES DE iNOS PARA AUMENTAR EL RENDIMIENTO VÍRICO EN CULTIVOS | |
| AR034216A1 (es) | Metodo de extraccion de virus a partir de cultivos celulares y composiciones que comprenden dicho virus | |
| NZ622731A (en) | Increasing virus-like particle yield in plants | |
| EA200701129A1 (ru) | Бактериальные упаковывающие штаммы, используемые для генерирования и продуцирования рекомбинантных нуклеокапсидов с двухцепочечной рнк и их применение | |
| AR043862A1 (es) | Procedimiento para la obtencion de estirpes celulares de mastocitos a partir de tejidos de cerdo y procedimiento para la produccion de moleculas de tipo de la heparina | |
| CL2013002723A1 (es) | Metodo de produccion de una particula semejante a virus (vlp) de alfavirus salmonido (sav) en celulas de insecto. | |
| BRPI0914805B8 (pt) | método para replicar vírus em uma cultura de células scldk, processo para adaptar células cldk dependentes do substrato para desenvolvimento em suspensão, método para propagar continuamente células scldk em suspensão, e, composição |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant, registration |