EA200701129A1 - Бактериальные упаковывающие штаммы, используемые для генерирования и продуцирования рекомбинантных нуклеокапсидов с двухцепочечной рнк и их применение - Google Patents

Бактериальные упаковывающие штаммы, используемые для генерирования и продуцирования рекомбинантных нуклеокапсидов с двухцепочечной рнк и их применение

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Publication number
EA200701129A1
EA200701129A1 EA200701129A EA200701129A EA200701129A1 EA 200701129 A1 EA200701129 A1 EA 200701129A1 EA 200701129 A EA200701129 A EA 200701129A EA 200701129 A EA200701129 A EA 200701129A EA 200701129 A1 EA200701129 A1 EA 200701129A1
Authority
EA
Eurasian Patent Office
Prior art keywords
recombinant
nukleokapsides
generation
production
application
Prior art date
Application number
EA200701129A
Other languages
English (en)
Other versions
EA011282B1 (ru
Inventor
Дэвид Майкл Хоун
Джон Фулкерсон
Джералд С. Сэдофф
Дэвид Ониэйб
Мишель Стоун
Original Assignee
ЭЙРАС ГЛОУБАЛ ТиБи ВЭКСИН ФАУНДЕЙШН
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ЭЙРАС ГЛОУБАЛ ТиБи ВЭКСИН ФАУНДЕЙШН filed Critical ЭЙРАС ГЛОУБАЛ ТиБи ВЭКСИН ФАУНДЕЙШН
Publication of EA200701129A1 publication Critical patent/EA200701129A1/ru
Publication of EA011282B1 publication Critical patent/EA011282B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/25—Shigella (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

Abstract

Настоящее изобретение относится к бактериальным упаковывающим штаммам, которые могут быть использованы для продуцирования рекомбинантных нуклеокапсидов, содержащих двухцепочечную РНК (rdsRN). Упаковывающие штаммы могут быть использованы для продуцирования РНК-кодирующих вакцинных антигенов, биологически активных белков, иммунорегуляторных белков, антисмысловых РНК и каталитических РНК в эукармотических клетках или тканях. Рекомбинантную ssPHK вводят в штаммы и пакуют до образования rdsRN de novo.
EA200701129A 2004-11-30 2005-11-23 Бактериальные упаковывающие штаммы, используемые для генерирования и продуцирования рекомбинантных нуклеокапсидов с двухцепочечной рнк, и их применение EA011282B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US99907404A 2004-11-30 2004-11-30
US71372905P 2005-09-06 2005-09-06
PCT/US2005/042480 WO2006060257A2 (en) 2004-11-30 2005-11-23 Bacterial packaging strains usefuel for generation and production of recombinant double-stranded rna nucleocapsids and uses thereof

Publications (2)

Publication Number Publication Date
EA200701129A1 true EA200701129A1 (ru) 2007-12-28
EA011282B1 EA011282B1 (ru) 2009-02-27

Family

ID=36565556

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200701129A EA011282B1 (ru) 2004-11-30 2005-11-23 Бактериальные упаковывающие штаммы, используемые для генерирования и продуцирования рекомбинантных нуклеокапсидов с двухцепочечной рнк, и их применение

Country Status (13)

Country Link
US (1) US8053568B2 (ru)
EP (1) EP1817408B1 (ru)
JP (1) JP2008521434A (ru)
KR (1) KR20070101854A (ru)
AP (1) AP2007004020A0 (ru)
AU (1) AU2005310119A1 (ru)
BR (1) BRPI0518109A (ru)
CA (1) CA2587493A1 (ru)
EA (1) EA011282B1 (ru)
MX (1) MX2007006366A (ru)
PE (1) PE20061169A1 (ru)
TW (1) TW200631593A (ru)
WO (1) WO2006060257A2 (ru)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69930147T2 (de) * 1998-12-04 2007-01-11 University Of Manitoba, Winnipeg Zwei-schritte-verfahren zur impfung gegen chlamydia
US7018835B2 (en) * 2002-08-20 2006-03-28 David Michael Hone Recombinant double-stranded RNA phage, and use of the same
US20080107683A1 (en) * 2004-11-30 2008-05-08 David Hone Bacterial Packaging Strains Useful for Generation and Production of Recombinant Double-Stranded RNA Nucleocapsids and Uses Thereof
US7829104B2 (en) * 2004-12-01 2010-11-09 Aeras Global Tb Vaccine Foundation Electroporation of Mycobacterium and overexpression of antigens in mycobacteria
CN1276076C (zh) * 2004-12-14 2006-09-20 华东理工大学 一种鳗弧菌野生毒株的无标记基因缺失减毒突变株及其应用
WO2008127486A2 (en) * 2006-12-29 2008-10-23 Bacilligen, Inc. Replication-proficient dsrna capsids and uses thereof
JP7098330B2 (ja) * 2015-06-10 2022-07-11 ホオキパ バイオテック ジーエムビーエイチ Ｈｐｖワクチン
CA3102078A1 (en) 2018-06-02 2019-12-05 Synlogic Operating Company, Inc. Bacteria engineered to treat liver disease

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5736388A (en) * 1994-12-30 1998-04-07 Chada; Sunil Bacteriophage-mediated gene transfer systems capable of transfecting eukaryotic cells
CA2231332C (en) * 1995-09-06 2007-04-17 Arthur A. Branstrom Bacterial delivery system
US6887483B2 (en) * 1995-12-01 2005-05-03 University Of Iowa Research Foundation Non-toxic mutants of pathogenic gram-negative bacteria
CA2305785A1 (en) * 1997-10-07 1999-04-15 University Of Maryland Biotechnology Institute Method for introducing and expressing rna in animal cells
JP4709545B2 (ja) * 2002-07-26 2011-06-22 ノバルティス バクシンズ アンド ダイアグノスティックス，インコーポレーテッド 修飾された低分子干渉ｒｎａ分子および使用方法
US7018835B2 (en) * 2002-08-20 2006-03-28 David Michael Hone Recombinant double-stranded RNA phage, and use of the same
US20040198640A1 (en) * 2003-04-02 2004-10-07 Dharmacon, Inc. Stabilized polynucleotides for use in RNA interference

Also Published As

Publication number Publication date
JP2008521434A (ja) 2008-06-26
EA011282B1 (ru) 2009-02-27
CA2587493A1 (en) 2006-06-08
EP1817408B1 (en) 2013-01-16
PE20061169A1 (es) 2006-11-08
MX2007006366A (es) 2008-03-10
EP1817408A2 (en) 2007-08-15
AU2005310119A1 (en) 2006-06-08
KR20070101854A (ko) 2007-10-17
EP1817408A4 (en) 2009-01-28
US8053568B2 (en) 2011-11-08
WO2006060257A2 (en) 2006-06-08
TW200631593A (en) 2006-09-16
WO2006060257A3 (en) 2006-12-14
US20060115493A1 (en) 2006-06-01
BRPI0518109A (pt) 2008-11-04
AP2007004020A0 (en) 2007-06-30

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU