BR112012015596A2 - "processo de produção de células aderentes e utilização das mesmas" - Google Patents

"processo de produção de células aderentes e utilização das mesmas"

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Publication number
BR112012015596A2
BR112012015596A2 BR112012015596A BR112012015596A BR112012015596A2 BR 112012015596 A2 BR112012015596 A2 BR 112012015596A2 BR 112012015596 A BR112012015596 A BR 112012015596A BR 112012015596 A BR112012015596 A BR 112012015596A BR 112012015596 A2 BR112012015596 A2 BR 112012015596A2
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BR
Brazil
Prior art keywords
cell
cells
microcarriers
adherent cells
cell population
Prior art date
Application number
BR112012015596A
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English (en)
Other versions
BR112012015596B1 (pt
Inventor
Eric Calvosa
Nicolas Seve
Original Assignee
Sanofi Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR1051754A external-priority patent/FR2957355B1/fr
Application filed by Sanofi Pasteur filed Critical Sanofi Pasteur
Publication of BR112012015596A2 publication Critical patent/BR112012015596A2/pt
Publication of BR112012015596B1 publication Critical patent/BR112012015596B1/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0056—Xeno-free medium
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12N2710/00011—Details
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    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
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    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20111—Lyssavirus, e.g. rabies virus
    • C12N2760/20151—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Zoology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Virology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
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  • Communicable Diseases (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

patente da invenção: "processo de produção de células aderentes e utilização das mesmas". a presente invenção refere-se a um processo de produção de células aderentes, segundo a qual: a. se introoduzem células aderentes em um primeiro recipiente de cultura que contém microportadores em um meio de cultura; b. se amplificam as células, efetuando-se várias passgens celulares sucessivas nesses mesmo recipiente de cultura, no qual cada passagem celular posterior à primeira passagem celular é realizada, i. utilizando-se toda ou parte da população celular que foi obtida, quando da passagem celular precedente após ter submetido a população celular a um tratamento enzimático para destacar as células dos microportadores,e ii. introduzindo-se o meio de cultura e uma quantidade crescente de microportadores, e c. coleta-se a população celular obtida, quando da última passagem celular, após ter de forma opcional submetido a população celular a um tratamento enzimático, para destacar as células dos microportadores. a invenção refere-se também à aplicação desse processo para a produção de agentes biológicos, servindo notadamente ao preoaro de vacinas ou de medicamentos.
BR112012015596-2A 2009-12-23 2010-12-21 Processo de cultura de células aderentes BR112012015596B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
FR0959472 2009-12-23
FR0959472 2009-12-23
FR1051754 2010-03-11
FR1051754A FR2957355B1 (fr) 2010-03-11 2010-03-11 Procede de culture de cellules adherentes a des microporteurs
PCT/FR2010/052850 WO2011077035A1 (fr) 2009-12-23 2010-12-21 Procede de culture de cellules adherentes

Publications (2)

Publication Number Publication Date
BR112012015596A2 true BR112012015596A2 (pt) 2015-11-03
BR112012015596B1 BR112012015596B1 (pt) 2021-06-01

Family

ID=43709027

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012015596-2A BR112012015596B1 (pt) 2009-12-23 2010-12-21 Processo de cultura de células aderentes

Country Status (14)

Country Link
US (1) US9145543B2 (pt)
EP (1) EP2516623B1 (pt)
JP (1) JP5894929B2 (pt)
KR (1) KR101835100B1 (pt)
CN (2) CN102782121A (pt)
AR (1) AR079710A1 (pt)
AU (1) AU2010334723C1 (pt)
BR (1) BR112012015596B1 (pt)
CA (1) CA2784741A1 (pt)
HK (1) HK1243731A1 (pt)
IL (1) IL220167B (pt)
MX (1) MX2012007117A (pt)
RU (1) RU2612154C2 (pt)
WO (1) WO2011077035A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140271633A1 (en) * 2013-03-14 2014-09-18 Abbvie Inc. Mammalian cell culture performance through surfactant supplementation of feed media
CN103289949A (zh) * 2013-06-28 2013-09-11 四川省华派生物制药有限公司 一种从克氏瓶直接到生物反应器的细胞培养方法
EP3122799B8 (en) 2014-03-25 2020-03-11 F.Hoffmann-La Roche Ag Methods of preparing a poloxamer for use in cell culture medium
KR102391259B1 (ko) * 2014-06-04 2022-04-26 암젠 인크 포유류 세포 배양물을 회수하는 방법
EP3475410A4 (en) 2016-06-24 2020-02-12 Lonza Ltd VARIABLE DIAMETER BIOREACTORS
CN106754672A (zh) * 2016-11-30 2017-05-31 广州赛莱拉干细胞科技股份有限公司 一种贴壁细胞的培养方法
CA3103570A1 (en) * 2018-06-14 2019-12-19 Abraham J And Phyllis Katz Cord Blood Foundation Isolation of mesenchymal stromal cells from umbilical cord blood
CN116036257A (zh) * 2022-12-29 2023-05-02 瑞普（保定）生物药业有限公司 一种猪瘟活疫苗的制备方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4664912A (en) 1984-10-01 1987-05-12 Wiktor Tadeusz J Process for the large scale production of rabies vaccine
FR2635008B1 (fr) 1988-08-05 1990-11-16 Sanofi Sa Agent activateur de la productivite specifique des cellules animales recombinantes a base de polyvinylpyrrolidone et milieu de culture defini le contenant
DK52393D0 (pt) 1993-05-05 1993-05-05 Novo Nordisk As
US5714375A (en) * 1995-06-05 1998-02-03 Nobl Laboratories, Inc. Ileal symbiont intracellularis propagation in suspended host cells
US6020182A (en) * 1996-07-12 2000-02-01 Connaught Laboratories Limited Subunit respiratory syncytial virus vaccine preparation
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US6168944B1 (en) * 1997-01-31 2001-01-02 Schering Corporation Methods for cultivating cells and propagating viruses
PT1801201E (pt) 1997-12-24 2011-07-05 Abbott Biologicals Bv Preparação de células para a produção de material biológico
FR2801900B1 (fr) 1999-12-03 2004-02-27 Pasteur Merieux Serums Vacc Methode de production de cellules animales adherentes
US6670184B2 (en) * 2000-06-23 2003-12-30 Basf Ag Method for producing a lipid emulsion for use in insect cell culture
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DE102004043256B4 (de) * 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
CN101265464B (zh) * 2008-05-13 2010-06-16 中国人民解放军第二军医大学 一种猪骨髓内皮祖细胞的分离和培养方法

Also Published As

Publication number Publication date
JP2013515473A (ja) 2013-05-09
RU2612154C2 (ru) 2017-03-02
US20110151506A1 (en) 2011-06-23
US9145543B2 (en) 2015-09-29
JP5894929B2 (ja) 2016-03-30
IL220167B (en) 2018-01-31
KR101835100B1 (ko) 2018-03-08
CA2784741A1 (fr) 2011-06-30
CN107460154A (zh) 2017-12-12
EP2516623A1 (fr) 2012-10-31
RU2012130928A (ru) 2014-01-27
EP2516623B1 (fr) 2016-10-05
HK1243731A1 (zh) 2018-07-20
KR20120102788A (ko) 2012-09-18
AR079710A1 (es) 2012-02-15
WO2011077035A1 (fr) 2011-06-30
BR112012015596B1 (pt) 2021-06-01
IL220167A0 (en) 2012-07-31
AU2010334723C1 (en) 2015-05-28
AU2010334723B2 (en) 2015-02-05
MX2012007117A (es) 2012-07-23
AU2010334723A1 (en) 2012-07-12
CN102782121A (zh) 2012-11-14

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