AU2010334723C1 - Method for culturing adherent cells - Google Patents

Method for culturing adherent cells Download PDF

Info

Publication number
AU2010334723C1
AU2010334723C1 AU2010334723A AU2010334723A AU2010334723C1 AU 2010334723 C1 AU2010334723 C1 AU 2010334723C1 AU 2010334723 A AU2010334723 A AU 2010334723A AU 2010334723 A AU2010334723 A AU 2010334723A AU 2010334723 C1 AU2010334723 C1 AU 2010334723C1
Authority
AU
Australia
Prior art keywords
cell
cells
microcarriers
culture
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2010334723A
Other languages
English (en)
Other versions
AU2010334723B2 (en
AU2010334723A1 (en
Inventor
Eric Calvosa
Nicolas Seve
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Pasteur Inc
Original Assignee
Sanofi Pasteur Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR1051754A external-priority patent/FR2957355B1/fr
Application filed by Sanofi Pasteur Inc filed Critical Sanofi Pasteur Inc
Publication of AU2010334723A1 publication Critical patent/AU2010334723A1/en
Publication of AU2010334723B2 publication Critical patent/AU2010334723B2/en
Application granted granted Critical
Publication of AU2010334723C1 publication Critical patent/AU2010334723C1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0056—Xeno-free medium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20111—Lyssavirus, e.g. rabies virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20111—Lyssavirus, e.g. rabies virus
    • C12N2760/20151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AU2010334723A 2009-12-23 2010-12-21 Method for culturing adherent cells Active AU2010334723C1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
FR0959472 2009-12-23
FR0959472 2009-12-23
FR1051754 2010-03-11
FR1051754A FR2957355B1 (fr) 2010-03-11 2010-03-11 Procede de culture de cellules adherentes a des microporteurs
PCT/FR2010/052850 WO2011077035A1 (fr) 2009-12-23 2010-12-21 Procede de culture de cellules adherentes

Publications (3)

Publication Number Publication Date
AU2010334723A1 AU2010334723A1 (en) 2012-07-12
AU2010334723B2 AU2010334723B2 (en) 2015-02-05
AU2010334723C1 true AU2010334723C1 (en) 2015-05-28

Family

ID=43709027

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010334723A Active AU2010334723C1 (en) 2009-12-23 2010-12-21 Method for culturing adherent cells

Country Status (14)

Country Link
US (1) US9145543B2 (https=)
EP (1) EP2516623B1 (https=)
JP (1) JP5894929B2 (https=)
KR (1) KR101835100B1 (https=)
CN (2) CN102782121A (https=)
AR (1) AR079710A1 (https=)
AU (1) AU2010334723C1 (https=)
BR (1) BR112012015596B1 (https=)
CA (1) CA2784741A1 (https=)
HK (1) HK1243731A1 (https=)
IL (1) IL220167B (https=)
MX (1) MX2012007117A (https=)
RU (1) RU2612154C2 (https=)
WO (1) WO2011077035A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140271633A1 (en) * 2013-03-14 2014-09-18 Abbvie Inc. Mammalian cell culture performance through surfactant supplementation of feed media
CN103289949A (zh) * 2013-06-28 2013-09-11 四川省华派生物制药有限公司 一种从克氏瓶直接到生物反应器的细胞培养方法
BR112016021739B1 (pt) * 2014-03-25 2021-11-03 Genentech, Inc Método para preparar um poloxâmero para uso em um meio de cultura de célula
PT3152317T (pt) * 2014-06-04 2019-04-18 Amgen Inc Métodos de colheita de culturas de células de mamíferos
JP7364334B2 (ja) 2016-06-24 2023-10-18 ロンザ リミテッド 直径可変型バイオリアクタ
CN106754672A (zh) * 2016-11-30 2017-05-31 广州赛莱拉干细胞科技股份有限公司 一种贴壁细胞的培养方法
WO2019240962A1 (en) * 2018-06-14 2019-12-19 Abraham J And Phyllis Katz Cord Blood Foundation Isolation of mesenchymal stromal cells from umbilical cord blood
CN115485363A (zh) * 2020-03-13 2022-12-16 昭和电工材料株式会社 细胞悬液的制造方法和黏附细胞的制造方法
EP4166644A1 (en) * 2020-06-12 2023-04-19 Biotech Foods S.L. Process and device for production of cultured meat
WO2022197878A1 (en) * 2021-03-19 2022-09-22 Merck Sharp & Dohme Llc A closed-system upstream manufacturing process for dengue virus production
CN116036257B (zh) * 2022-12-29 2024-11-15 瑞普（保定）生物药业有限公司 一种猪瘟活疫苗的制备方法
WO2025125314A1 (fr) 2023-12-11 2025-06-19 Commissariat A L'energie Atomique Et Aux Energies Alternatives Dispositif et procédé de culture cellulaire

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4664912A (en) 1984-10-01 1987-05-12 Wiktor Tadeusz J Process for the large scale production of rabies vaccine
FR2635008B1 (fr) 1988-08-05 1990-11-16 Sanofi Sa Agent activateur de la productivite specifique des cellules animales recombinantes a base de polyvinylpyrrolidone et milieu de culture defini le contenant
DK52393D0 (https=) 1993-05-05 1993-05-05 Novo Nordisk As
US5714375A (en) * 1995-06-05 1998-02-03 Nobl Laboratories, Inc. Ileal symbiont intracellularis propagation in suspended host cells
US6020182A (en) * 1996-07-12 2000-02-01 Connaught Laboratories Limited Subunit respiratory syncytial virus vaccine preparation
DE69720024D1 (de) * 1996-07-12 2003-04-24 Aventis Pasteur Immunisierungsverfahren in zwei schritten gegen pyramyxoviridae-viren unter verwendung abgeschwächter viraler stämme und preparation von proteinuntereinheiten
US6168944B1 (en) * 1997-01-31 2001-01-02 Schering Corporation Methods for cultivating cells and propagating viruses
ATE356197T1 (de) 1997-12-24 2007-03-15 Duphar Int Res Herstellung von zellen für die produktion von biologischen produkten
FR2801900B1 (fr) 1999-12-03 2004-02-27 Pasteur Merieux Serums Vacc Methode de production de cellules animales adherentes
US6670184B2 (en) * 2000-06-23 2003-12-30 Basf Ag Method for producing a lipid emulsion for use in insect cell culture
US6951752B2 (en) * 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen
US6855535B2 (en) * 2001-12-10 2005-02-15 Baxter Healthcare S.A. Method of large scale production of Hepatitis A virus
DE102004043256B4 (de) * 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
CN101265464B (zh) * 2008-05-13 2010-06-16 中国人民解放军第二军医大学 一种猪骨髓内皮祖细胞的分离和培养方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
LANDAUER, K. et al., Cytotechnology, 2002, Vol. 39, pages 37-45 *
OHLSON S. et al., Cytotechnology, 1994, Vol. 14, pages 67-80 *
ROUROU, S. et al., Applied Microbiology and Biotechnology, 2009, Vol. 85, pages 53-63 *
WANG Y. et al., Cytotechnology, 1999, Vol. 31, pages 221-224 *
XIAO C et al., Chinese Academy of Medical Sciences, 1994, Vol. 9, pages 71-74 *

Also Published As

Publication number Publication date
JP2013515473A (ja) 2013-05-09
HK1243731A1 (zh) 2018-07-20
CN107460154A (zh) 2017-12-12
CN102782121A (zh) 2012-11-14
US20110151506A1 (en) 2011-06-23
BR112012015596A2 (pt) 2015-11-03
KR20120102788A (ko) 2012-09-18
RU2612154C2 (ru) 2017-03-02
WO2011077035A1 (fr) 2011-06-30
MX2012007117A (es) 2012-07-23
KR101835100B1 (ko) 2018-03-08
EP2516623B1 (fr) 2016-10-05
CA2784741A1 (fr) 2011-06-30
BR112012015596B1 (pt) 2021-06-01
AR079710A1 (es) 2012-02-15
AU2010334723B2 (en) 2015-02-05
US9145543B2 (en) 2015-09-29
IL220167B (en) 2018-01-31
IL220167A0 (en) 2012-07-31
AU2010334723A1 (en) 2012-07-12
JP5894929B2 (ja) 2016-03-30
RU2012130928A (ru) 2014-01-27
EP2516623A1 (fr) 2012-10-31

Similar Documents

Publication Publication Date Title
AU2010334723C1 (en) Method for culturing adherent cells
Rourou et al. A microcarrier cell culture process for propagating rabies virus in Vero cells grown in a stirred bioreactor under fully animal component free conditions
JP5264812B2 (ja) Ｍｄｃｋ細胞懸濁培養物において薬物または診断剤の活性成分を生成する方法
JP4787739B2 (ja) 動物を含まない細胞培養方法
JP2010246559A (ja) 細胞培養物中のウイルス増殖
JP2013515473A5 (https=)
CN103827296A (zh) 用于产生病毒抗原和疫苗的方法
US10851350B1 (en) Bioreactor production of virus from adherent cells
Sousa et al. Process intensification for Peste des Petites Ruminants Virus vaccine production
CN112280740A (zh) 一株稀有鮈鲫脑细胞系及其应用
US9932562B2 (en) Drain down and re-feed of microcarrier bioreactor
WO2022071966A1 (en) Bioreactor production of virus from adherent cells
CN118021946A (zh) 一种利用生物反应器制备甲型肝炎灭活疫苗的方法
KR20190019776A (ko) 무혈청배지 부유배양에 적응된 광견병 바이러스 생산 세포주
Elries et al. Chromosomes Stability and Growth Assessment of a Newly Established Lamb Heart Cell Line Sensitive to Lumpy Skin Disease Virus
FR2957355A1 (fr) Procede de culture de cellules adherentes a des microporteurs

Legal Events

Date Code Title Description
DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 19 FEB 2015 .

DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 19 FEB 2015

FGA Letters patent sealed or granted (standard patent)