BR0014496A - Proteìna, dna, e, método para a produção de l-aminoácido - Google Patents

Proteìna, dna, e, método para a produção de l-aminoácido

Info

Publication number
BR0014496A
BR0014496A BR0014496-7A BR0014496A BR0014496A BR 0014496 A BR0014496 A BR 0014496A BR 0014496 A BR0014496 A BR 0014496A BR 0014496 A BR0014496 A BR 0014496A
Authority
BR
Brazil
Prior art keywords
amino acid
primers
dna
enzyme
corynebacterium
Prior art date
Application number
BR0014496-7A
Other languages
English (en)
Inventor
Seiko Hirano
Gen Nonaka
Yumi Matsuzaki
Naoki Akiyoshi
Kanae Nakamura
Eiichiro Kimura
Tsuyoshi Osumi
Kazuhiko Matsui
Yoshio Kawahara
Osamu Kurahashi
Tsuyoshi Nakamtsu
Shinichi Sugimoto
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of BR0014496A publication Critical patent/BR0014496A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C12Y108/01004—Dihydrolipoyl dehydrogenase (1.8.1.4), i.e. lipoamide-dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01041—Isocitrate dehydrogenase (NAD+) (1.1.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01051—Pyruvate dehydrogenase (NADP+) (1.2.1.51)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04002—Oxoglutarate dehydrogenase (succinyl-transferring) (1.2.4.2), i.e. alpha-ketoglutarat dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01004—Glutamate dehydrogenase (NADP+) (1.4.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03001—Citrate (Si)-synthase (2.3.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01011—6-Phosphofructokinase (2.7.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01031—Phosphoenolpyruvate carboxylase (4.1.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/03—Oxo-acid-lyases (4.1.3)
    • C12Y401/03001—Isocitrate lyase (4.1.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01003—Aconitate hydratase (4.2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01001—Pyruvate carboxylase (6.4.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

"PROTEìNA, DNA, E, MéTODO PARA A PRODUçãO DE L-AMINOACIDO". Uma pluralidade de grupos iniciadores são designados com base em uma região onde a conservação no nível de aminoácido é observada entre vários microorganismos para as seq³ências de gene conhecidas correspondendo a um gene que codifica uma enzima da via biossintética de L-aminoácido derivado de Corynebacterium thermoaminogenes, preferivelmente uma que funciona em uma temperatura mais elevada comparada com aquela do Corynebacterium glutamicum. O PCR é executado mediante o uso dos iniciadores e DNA cromossómico de Corynebacterium thermoaminogenes como um modelo. Os iniciadores com os quais um fragmento de amplificação foi obtido são usados como iniciadores de peneiramento para selecionar um clone contendo um fragmento de DNA alvo a partir de uma livraria de plasmídeo de DNA cromossómico de Corynebacterium thermoaminogenes.
BR0014496-7A 1999-10-04 2000-10-04 Proteìna, dna, e, método para a produção de l-aminoácido BR0014496A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP28271699 1999-10-04
JP31114799 1999-11-01
JP2000120687 2000-04-21
PCT/JP2000/006913 WO2001025447A1 (fr) 1999-10-04 2000-10-04 Gene enzymatique du systeme de biosynthese d'acide amine thermophile

Publications (1)

Publication Number Publication Date
BR0014496A true BR0014496A (pt) 2002-08-20

Family

ID=27336960

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0014496-7A BR0014496A (pt) 1999-10-04 2000-10-04 Proteìna, dna, e, método para a produção de l-aminoácido

Country Status (12)

Country Link
US (3) US6995250B1 (pt)
EP (3) EP1219712B1 (pt)
JP (1) JP4337298B2 (pt)
KR (1) KR20020042703A (pt)
CN (3) CN101096662A (pt)
AT (3) ATE450612T1 (pt)
AU (1) AU781863B2 (pt)
BR (1) BR0014496A (pt)
DE (3) DE60043465D1 (pt)
PE (1) PE20010711A1 (pt)
TW (3) TW200734459A (pt)
WO (1) WO2001025447A1 (pt)

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JP4427878B2 (ja) 1999-08-20 2010-03-10 味の素株式会社 析出を伴う発酵法によるｌ−グルタミン酸の製造法
EP1106622A3 (de) * 1999-11-23 2004-01-02 Degussa AG Neue für das pfkA-Gen codierende Nukleotidsequenzen
DE10011922A1 (de) 1999-11-23 2001-05-31 Degussa Neue für das pfkA-Gen codierende Nukleotidsequenzen
PT1325135E (pt) * 2000-10-13 2005-03-31 Archer Daniels Midland Co Gene da carboxilase do piruvato de corynebacterium resistente a retroacao
JP4599726B2 (ja) 2001-02-20 2010-12-15 味の素株式会社 Ｌ−グルタミン酸の製造法
BR0304860A (pt) * 2002-11-11 2004-08-31 Ajinomoto Kk Método para produzir uma substância alvo pela utilização de uma-bactéria pertencente ao gênero escherichia
US7205132B2 (en) * 2004-09-10 2007-04-17 Ajinomoto Co., Inc. L-glutamic acid-producing microorganism and a method for producing L-glutamic acid
US9725701B2 (en) 2013-03-15 2017-08-08 Iowa Corn Promotion Board Prokarytoic-type isocitrate dehydrogenase and its application for improving nitrogen utilization in transgenic plants
KR101776375B1 (ko) * 2015-03-18 2017-09-08 씨제이제일제당 (주) 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법
CN106544298B (zh) * 2016-10-27 2020-03-24 广东省微生物研究所 一种芽胞杆菌感受态细胞的制备方法
CN118063566A (zh) * 2022-11-22 2024-05-24 华东理工大学 一种磷酸烯醇式丙酮酸光学探针及其制备方法和应用

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FR2575492B1 (fr) 1984-12-27 1988-09-16 Asahi Chemical Ind Fragment d'adn contenant un gene codant pour la glutamate deshydrogenase, adn recombinant le contenant, et microorganisme contenant l'adn recombinant
JPH07121228B2 (ja) 1986-11-07 1995-12-25 協和醗酵工業株式会社 Ｌ−グルタミン酸およびｌ−プロリンの製造法
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JP7121227B2 (ja) * 2017-06-29 2022-08-18 三菱マテリアル株式会社 熱電変換材料、及び、熱電変換材料の製造方法
JP7063383B2 (ja) * 2018-06-08 2022-05-09 株式会社島津製作所 蛍光ｘ線分析装置および蛍光ｘ線分析方法

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Publication number Publication date
WO2001025447A1 (fr) 2001-04-12
CN1391612A (zh) 2003-01-15
EP1712632A3 (en) 2006-10-25
AU7556100A (en) 2001-05-10
US7125977B2 (en) 2006-10-24
KR20020042703A (ko) 2002-06-05
JP4337298B2 (ja) 2009-09-30
EP1712632B1 (en) 2009-12-02
US20050176127A1 (en) 2005-08-11
TW200734459A (en) 2007-09-16
EP1710313B1 (en) 2009-12-02
CN101096661B (zh) 2011-10-19
PE20010711A1 (es) 2001-07-09
CN101096661A (zh) 2008-01-02
EP1219712A4 (en) 2004-06-02
ATE450613T1 (de) 2009-12-15
EP1712632A2 (en) 2006-10-18
TWI289604B (en) 2007-11-11
US7183403B2 (en) 2007-02-27
DE60043465D1 (de) 2010-01-14
ATE450612T1 (de) 2009-12-15
ATE396272T1 (de) 2008-06-15
EP1710313A1 (en) 2006-10-11
DE60043464D1 (de) 2010-01-14
AU781863B2 (en) 2005-06-16
DE60038967D1 (en) 2008-07-03
CN1333078C (zh) 2007-08-22
US20050239176A1 (en) 2005-10-27
US6995250B1 (en) 2006-02-07
TW200734460A (en) 2007-09-16
EP1219712B1 (en) 2008-05-21
CN101096662A (zh) 2008-01-02
EP1219712A1 (en) 2002-07-03

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