AU2021101555A4 - Method for cutting and planting Sanguisorba officinalis - Google Patents

Abstract

The present invention relates to a method for cutting and planting of Sanguisorba officinalis, including following steps: 1) Preparation of a planting bed: arranging a sterilized substrate in the planting bed, the substrate including river sand, coarse sand and pebbles; 2) Preparation of cuttings: cutting lignified stem segments as cuttings from June to September, cut the cuttings into short sections, disinfecting, and performing hormone treatment; 3) Planting: inserting the prepared cuttings into the planting bed at an inclination of 75; 4) Management after planting: after planting, conducting weeding and pest control treatments, and controlling relative air humidity and temperature; 5) Transplanting: Transplanting when the root length is 3-5cm. The invention has advantages that survival rate of cuttings can reach more than 80%, the root system is well developed, the survival rate after transplanting exceeds 90%, and growth thereof is good; and the present invention involves low cost, easy operation, achieves high survival rate and is suitable for popularization. 1/1 Figure 1

Description

1/1

Figure 1

Method for cutting and planting Sanguisorba officinalis

Technical field

The present invention relates to the technical field of vegetative propagation of

Sanguisorba, and more particularly relates to a method for cutting and planting

Sanguisorba officinalis.

Background technology

Sanguisorba officinalis L. is a perennial herbaceous plant of the Rosaceae family;

there are about thirty-five plant species of Sanguisorba all over the world, seven

species and six varieties in China, and mainly distributed in Northeast, North,

Northwest, East and Central South of China, etc..

Sanguisorba officinalis is a wild plant that integrates medicinal, edible and

ornamental functions. It is also a kind of traditional Chinese medicine, and its young

leaves are edible, and it is also a wild flower with high ornamental value. Contours

and colors of its flowers and shapes of the leaves are beautiful, with a large number

of flowers and a long flowering period. In addition, it can be used in flower parks, as

potted plants or in flower arranging, with a huge application potential. The vitality of

S. officinalis is strong, and the cultivation conditions are not strict. The underground

part thereof is cold-resistant, and the above-ground part is tolerant to high

temperature and rain. It can be cultivated in all parts of the north and south of China.

Because of its high medicinal value, economic benefits and ornamental value, it is

worthy of popularization and application as an artificially cultivated flower, but it is

still in the wild and needs to be developed urgently.

In nature, the propagation of Sanguisorba officinalis is done by sowing or plant

division, but both of these two methods have some defects. Although the

propagation coefficient of sowing and division is high, the seedlings grow slowly, and

the flowering cycle is long, making it difficult to achieve mass breeding. Moreover, some wild species have a low seed setting rate and uneven seedling quality; although by plant division seedlings multiply quickly and have a high survival rate, it is rarely used in mass production, and it is difficult to maintain good properties of parent plants. Cutting propagation is an important means of vegetive propagation of plants as its seedling cycle is short, low cost is involved, and a wide source of propagation materials isavailable, furthermore, it is convenient for use in mass propagation, excellent traits of the parent plants can be maintained and it is widely used in the cultivation of new varieties and the preservation of germplasm resources. Therefore, existing methods for the propagation of S. officinalis have deficiencies such as long seeding reproduction cycle, high culturing and reproduction cost,cumbersome operation, and difficult technical management and at present, there are no reports on the cutting propagation technology of S. officinalis. Consequently, carrying out researches on cutting propagation technology of S. officinalis will be of great significance to the preservation, promotion, and application of improved varieties of

S. officinalis.

Summary of the invention

The technical problem to be solved by the present invention is to provide a simple

and effective method for cutting and planting of Sanguisorba officinalis. The present

invention solves the above technical problems through the following technical

solutions:

A method for cutting and planting Sanguisorba officinalis, including following steps:

1) Preparation of a planting bed: arranging sterilized substrate in the planting bed,

the substrate including river sand, coarse sand and pebbles;

2) Preparation of cuttings: cutting the lignified stem segments as cuttings from June

to September, cutting the cuttings into sections with a length of 5-12 cm, disinfecting

the cutting sections, cleaning and giving hormone treatment;

3) Planting: digging holes or slots in the substrate, inserting the hormone-treated cutting sections into holes or channels at an inclination of 75, and pouring water thoroughly after planting;

4) Management after planting: after planting, giving weed and pest control

treatments within one to five days after planting, pouring water in the morning and

evening every day, meanwhile, maintaining light transmittance of 80-90%, relative air

humidity of 70-90%, and temperature of 18-380 C, and fifteen days after planting,

keep the relative air humidity at 60-70%, when damaged tissue recovery or rooting

begins, spraying urea water solution with a mass percentage of 0.2%;

) Transplanting: when the root length is 3-5cm, transplanting with the substrate

remaining on the root system, watering thoroughly after transplanting, and spraying

nutrient solution every seven to ten days after one month.

Preferably, in the step 1), the substrate has three layers, an upper layer is river sand

with a thickness of 10-15 cm, which has good air permeability and water retention

property, an intermediate layer is coarse sand with a thickness of 20 cm, and a

bottom layer is pebbles with a thickness of 15 cm to increase water permeability.

Preferably, a method of disinfection treatment is to expose and disinfect river sand

for two days, and use 400-800mg/kg potassium permanganate solution to disinfect

river sand, coarse sand and pebbles for three days one week before planting, and

then rinse with water.

Preferably, in the step 2), the stem segments are ground stems or stem segments

with pinnate leaves, and thickness of the stem segments is 0.2-0.5 cm.

Preferably, in the step 2), put the cuttings immediately into water after cutting.

Preferably, in the step 2), an upper end of the cuttings is flat and 1 cm away from the

buds, with one to two small leaves, and when the leaves are large, reducing 1/2 or

1/3; a lower end thereof is a horse-ear-shaped oblique end with 1-2 young buds.

Preferably, in the step 2), a method of disinfection treatment is to soak upper ends

and lower ends of the cuttings with 500 times carbendazim liquid 0.3% for one to three minutes.

Preferably, in the step 2), the hormone treatment method is to soak the cleaned

lower ends of the cuttings with 50-300mg/L rooting powder for one to three min.,

and rooting powder is prepared from a 50% alcohol solution by volume.

Preferably, in the step 4), the pest control method is spraying cuttings with 500 times

thiophanate methyl every eight to twelve days, and spraying the substrate with 500

times carbendazim every five to nine days.

Preferably, in the step 5), the nutrient solution contains urea with a mass percentage

of 0-0.1% and potassium dihydrogen phosphate with a mass percentage of 0.1-0.5%,

and a dosage thereof is 1-2L/m 2 .

Compared with the prior art, the present invention has the following advantages:

The present invention provides a complete method for vegetative propagation and

seedling raising by cutting and planting of the stem segments of Sanguisorba

officinalis, preparing suitable planting beds, selecting suitable cuttings, suitable

concentration of plant growth regulators and treatment time; and correct cutting

methods can promote root growth of the cuttings quickly, and increase effectively

rooting rate and root length. In addition, the method of the present invention is

simple and easy to implement, the required tools are cheap, the cost is low, the

cutting materials are easy to obtain, the rooting rate after planting is high, and the

effect is remarkable. It provides a simple and effective method for the vegetative

reproduction and seedling cultivation of Sanguisorba officinalis, which has strong

practicability and maneuverability. The survival rate of the cultivated cuttings can

reach more than 80%. Planted stem segments have a well-developed root system

and can be transplanted out of the garden within one month. The survival rate of the

transplanted seedlings exceeds 90%, and the growth thereof is good. The present

invention establishes a relatively complete technical system for the cutting and

planting of S. officinalis, lays a foundation for the construction of great burnet picking

gardens and cultivating improved varieties and new varieties, etc., and strengthens protection and utilization of wild plants.

Description of drawings

Fig. 1 is a diagram showing cutting and planting of Sanguisorba officinalis according

to the present invention.

Detailed Description

In the following paragraphs, embodiments of the present invention are described in

details. The embodiments are implemented on the premise of the technical solution

of the present invention, and provide detailed implementation modes and specific

operation procedures of the present invention, but the scope of protection of the

present invention is not limited to the following implementations.

Embodiment 1

The method for cutting and planting Sanguisorba officinalis includes following steps:

(1) Selection of a nursery land: choose a place with open terrain, deep soil layer and

fertile soil as the nursery land for cutting and planting.

(2) Preparation of a planting bed: the planting bed is surrounded by cement in an

arch shed with double-layer films covered with plastic film,, rectangular in east-west

orientation, leeward, facing sun and enjoying sufficient sunlight. An upper part of the

planting bed is shaded by 80% shading net, the planting bed is 40cm above the

ground, and the planting bed is equipped with artificial spraying facilities. The

planting bed has a thickness of 50cm, and the planting bed consists of three layers.

An upper layer is river sand with a thickness of 12cm, which has good air

permeability and a good water retention property. River sand used has been exposed

in sunlight for two days, which has a certain sterilization effect; under the river sand

is coarse sand with a thickness of 20cm, and the bottom layer is covered with pebbles with a thickness of 15cm to increase water permeability. Treat the planting bed (including river sand, coarse sand and pebbles) with 500mg/kg potassium permanganate solution one week before planting, and rinse thoroughly with water after three days of treatment.

(3) Preparation of cuttings: in July of each year, select robust, disease-free, and

lignified Sanguisorba officinalis plants, and use sharp scissors to cut the lignified

ground stems or stems with pinnate leaves with a diameter of about 0.2cm in the

early morning or evening. Put the stems immediately in a bucket filled with distilled

water. Among them, the scissors and the bucket are sprayed and disinfected with 75%

alcohol.

The stems after cutting should be processed in time, to remove tender parts at spikes,

and cut into short cuttings with a length of 8cm. And then upper ends of the cuttings

is flat and 1 cm away from the buds, with one to two small leaves, and when the

leaves are large, reducing 1/2 or 1/3; lower ends thereof are horse-ear-shaped

oblique ends with one to two young buds. Bundle the prepared cuttings in a neat

manner with the flat parts on the top and the oblique parts on the bottom. Each fifty

roots are tied into a bundle, and the upper and lower ends of the cuttings are

disinfected by adding 0.3% carbendazim (500 times solution). After soaking for two

minutes, wash with distilled water twice. Soak the cleaned lower end of the cuttings

with 50mg/L ABT2 rooting powder (purchased from Beijing Aibiti Biotechnology Co.,

Ltd.) for one min, with soaking depth 1-3cm, and the ABT rooting powder is prepared

with a 50% alcohol solution by volume.

(4) Planting: when planting, use wooden sticks to draw holes or slots 2-4cm deep,

planting depth 3cm, plant spacing 4cm, row spacing 5cm, cuttings inclination 75,

bury the cuttings after cutting and compact the surrounding substrate with both

hands. Keep the cuttings in close contact with the substrate, and water the cuttings

once immediately after finishing planting.

(5) Post-planting management: after planting, weeds should be timely weeded on cloudy and rainy days, and attention should be paid to disease prevention and pest control. Pouring water twice a day, in the early morning and evening respectively, and cover with plastic film to make the light transmittance in the shed 80%. Fully automatic spraying device is used to spray five to six times a day to keep the relative air humidity at 80% and the air temperature at 25-30C. When the temperature in the shed is too high, open two ends of the plastic film to ventilate and cool down.

When the light is too strong at noon, spread the shade net and the plastic film.

Fifteen days after planting, appropriately reduce number of sprays to three to four

times a day, and the relative air humidity is controlled at 70%. When the callus

growth or rooting begins, spray 0.2% urea water solution to supply the nutrients

needed by the plants. Spray thiophanate-methyl (500 times solution) every ten days

to prevent cuttings from going moldy, check temperature and humidity every day,

and remove rotten cuttings in time. In order to prevent the base of the cuttings from

rotting, spray 500 times carbendazim through the substrate every seven days after

planting. The spraying time is in the evening after water spraying. Observe the

growth of the cuttings at any time.

(6) Transplanting after planting: one month after planting, the root length is 3-5cm,

the rooting rate is 90%, the root system is well developed, and pull out the cuttings in

the early morning or evening; growth status of the cuttings is shown in Figure 1, the

survival rate is 85%, and transplant the cuttings in pots or fields within one day after

pulling out. The day before transplanting, do not spray to avoid over-wetting the

substrate; when transplanting, try to preserve the substrate on the root system;

when potting, do not compact the substrate, water thoroughly in time to ensure that

the cuttings are in close contact with the soil to recover the seedlings; two days after

transplanting, water again, then carry out routine water management, fertilize for

the first time after one month, and then spray the nutrient solution once every seven

days, the nutrient solution formula is 0.1% urea by mass percentage and potassium

dihydrogen phosphate with 0.1% mass percentage. The spraying time of the nutrient

solution is to spray with a sprayer after water spraying, and the dosage is 1.5L/m 2 .

Observe the growth of transplanted seedlings, and it is observed that the survival

rate of transplanted seedlings is 94% after three months.

Embodiment 2

The method for cutting and planting Sanguisorba officinalis includes following steps:

(1) Selection of a nursery land: choose a place with open terrain, deep soil layer and

fertile soil as the nursery land for cutting and planting.

(2) Preparation of a planting bed: the planting bed is surrounded by cement in an

arch shed with double-layer films covered with plastic film, rectangular in east-west

orientation, leeward, facing sun and enjoying sufficient sunlight. An upper part of the

planting bed is shaded by 80% shading net, the planting bed is 40cm above the

ground, and the planting bed is equipped with artificial spraying facilities. The

planting bed has a thickness of 50cm, and the planting bed consists of three layers.

An upper layer is river sand with a thickness of 10cm, which has good air

permeability and a good water retention property. River sand used has been exposed

in sunlight for two days, which has a certain sterilization effect; under the river sand

is coarse sand with a thickness of 20cm, and the bottom layer is covered with

pebbles with a thickness of 15cm to increase water permeability. Treat the planting

bed (including river sand, coarse sand and pebbles) with 800mg/kg potassium

permanganate solution one week before planting, and rinse thoroughly with water

after three days of treatment.

(3) Preparation of cuttings: In August of each year, select robust, disease-free, and

lignified Sanguisorba officinalis plants, and use sharp scissors to cut the lignified

ground stems with a diameter of 0.4cm or stems with pinnate leaves with a diameter

of about 0.2cm in the early morning or evening. Put the stems immediately in a

bucket filled with distilled water. Among them, the scissors and the bucket have been

sprayed and disinfected with 75% alcohol.

The stems after cutting should be processed in time, to remove the tender parts at spikes, and cut into short cuttings with a length of 8cm. And then upper ends of the cuttings are flat and 1cm away from the buds, with one to two small leaves, and when the leaves are large, reducing 1/2 or 1/3; lower ends thereof are horse-ear-shaped oblique ends with one to two young buds. Bundle the prepared cuttings in a neat manner with the flat parts on the top and the oblique parts on the bottom. Each fifty roots are tied into a bundle, and the upper and lower ends of the cuttings are disinfected by adding 0.3% carbendazim (500 times solution). After soaking for two minutes, wash with distilled water twice. Soak the cleaned lower ends of the cuttings with 100mg/L ABT2 rooting powder (purchased from Beijing

Aibiti Biotechnology Co., Ltd.) for one min, with soaking depth 1-3cm, and the ABT

rooting powder is prepared with a 50% alcohol solution by volume.

(4) Planting: when planting, use wooden sticks to draw holes or slots 2-4cm deep,

planting depth 3cm, plant spacing 4cm, row spacing 5cm, cuttings inclination 75,

bury the cuttings after cutting and compact the surrounding substrate with both

hands. Keep the cuttings in close contact with the substrate, and water the cuttings

once immediately after the cuttings are finished.

(5) Post-planting management: after planting, weeds should be timely weeded on

cloudy and rainy days, and attention should be paid to disease prevention and pest

control. Pouring water twice a day, in the early morning and evening respectively,

and cover with plastic film to make the light transmittance in the shed 80%. Fully

automatic spraying device is used to spray five to six times a day to keep the relative

air humidity at 90% and the air temperature at 25-30C. When the temperature in

the shed is too high, open two ends of the plastic film to ventilate and cool down.

When the light is too strong at noon, spread the shade net and the plastic film.

Fifteen days after planting, appropriately reduce number of sprays to three to four

times a day, and the relative air humidity is controlled at 70%. When the callus

growth or rooting begins, spray 0.2% urea water solution to supply the nutrients

needed by the plants. Spray thiophanate-methyl (500 times solution) every eight

days to prevent cuttings from going moldy, check temperature and humidity every day, and remove rotten cuttings in time. In order to prevent the base of the cuttings from rotting, spray 500 times carbendazim through the substrate every seven days after planting. The spraying time is in the evening after water is sprayed. Observe the growth of the cuttings at any time.

(6) Transplanting after planting: one month after planting, the root length is 3-5cm,

the rooting rate is 87%, the root system is well developed, and pull out the cuttings in

the early morning or evening. Transplant the cuttings in pots or fields within one day

after pulling out. The day before transplanting, do not spray to avoid over-wetting

the substrate; when transplanting, try to preserve the substrate on the root system;

when potting, do not compact the substrate, water thoroughly in time to ensure that

the cuttings are in close contact with the soil to recover the seedlings; two days after

transplanting, water again, then carry out routine water management, fertilize for

the first time after one month, and then spray the nutrient solution once every ten

days, the nutrient solution formula is 0.5% urea by mass percentage and potassium

dihydrogen phosphate with 0.1% mass percentage. The spraying time of the nutrient

solution is to spray with a sprayer after water spraying, and the dosage is 1L/m2

. Observe the growth of transplanted seedlings, and it is observed that the survival

rate of transplanted seedlings is 91% after three months.

Embodiment 3

The method for cutting and planting Sanguisorba officinalis includes the following

steps:

(1) Selection of a nursery land: choose a place with open terrain, deep soil layer and

fertile soil as the nursery land for cutting and planting.

(2) Preparation of a planting bed: the planting bed is surrounded by cement in an

arch shed with double-layer films covered with plastic film, rectangular in east-west

direction, leeward, facing sun and enjoying sufficient sunlight. An upper part of the

planting bed is shaded by 80% shading net, the planting bed is 40cm above the ground, and the planting bed is equipped with artificial spraying facilities. The planting bed has a thickness of 50cm, and the planting bed consists of three layers.

An upper layer is river sand with a thickness of 15cm, which has good air

permeability and a good water retention property. River sand used has been exposed

in sunlight for two days, which has a certain sterilization effect; under the river sand

is coarse sand with a thickness of 20cm, and the bottom layer is covered with

pebbles with a thickness of 15cm to increase water permeability. Treat the planting

bed (including river sand, coarse sand and pebbles) with 600mg/kg potassium

permanganate solution one week before planting, and rinse thoroughly with water

after three days of treatment.

(3) Preparation of cuttings: In September of each year, select robust, disease-free,

and lignified Sanguisorba officinalis plants, and use sharp scissors to cut the lignified

ground stems with a diameter of 0.4cm or stems with pinnate leaves with a diameter

of about 0.3cm in the early morning or evening. Put the stems immediately in a

bucket filled with distilled water. Among them, the scissors and the bucket have been

sprayed and disinfected with 75% alcohol.

The stems after cutting should be processed in time, to remove the tender parts at

spikes, and cut them into short cuttings with a length of 12cm. And then upper ends

of the cuttings are flat and 1 cm away from the buds, with one to two small leaves,

and when the leaves are large, reducing 1/2 or 1/3; lower ends thereof area

horse-ear-shaped oblique end with one to two young buds. Bundle the prepared

cuttings in a neat manner with the flat parts on the top and the oblique parts on the

bottom. Each fifty roots are tied into a bundle, and the upper and lower ends of the

cuttings are disinfected by adding 0.3% carbendazim (500 times solution). After

soaking for two minutes, wash with distilled water twice. Soak the cleaned lower end

of the cuttings with 200mg/L ABT2 rooting powder (purchased from Beijing Aibiti

Biotechnology Co., Ltd.) for one min, with soaking depth 1-3cm, and the ABT rooting

powder is prepared with a 50% alcohol solution by volume.

(4) Planting: when planting, use wooden sticks to draw holes or slots 2-4cm deep, planting depth 3cm, plant spacing 4cm, row spacing 5cm, cuttings inclination 75, bury the cuttings after cutting and compact the surrounding substrate with both hands. Keep the cuttings in close contact with the substrate, and water the cuttings once immediately after the cuttings are finished.

(5) Post-planting management: after planting, weeds should be timely weeded on

cloudy and rainy days, and attention should be paid to disease prevention and pest

control. In the first five days after planting, pouring water twice a day in the early

morning and evening respectively, and cover with plastic film to make the light

transmittance in the shed 80%. Fully automatic spraying device is used to spray five

to six times a day to keep the relative air humidity at 90% and the air temperature at

-30C. When the temperature in the shed is too high, open two ends of the plastic

film to ventilate and cool down. When the light is too strong at noon, spread the

shade net and the plastic film. Fifteen days after planting, appropriately reducing the

number of sprays to three to four times a day, and the relative air humidity is

controlled at 60%. When the callus growth or rooting begins, spray 0.2% urea water

solution to supply the nutrients needed by the plants. Spray thiophanate-methyl (500

times solution) every twelve days to prevent cuttings from going moldy, check

temperature and humidity every day, and remove rotten cuttings in time. In order to

prevent the base of the cuttings from rotting, spray 500 times carbendazim through

the substrate every nine days after planting. The spraying time is in the evening after

water is sprayed. Observe the growth of the cuttings at any time.

(6) Transplanting after planting: one month after planting, the root length is 3-5cm,

the rooting rate is 85%, the root system is well developed, pull out the cuttings in the

early morning or evening, and the a survival rate is 80%. Transplant the cuttings in

pots or fields within one day after seedling emergence. The day before transplanting,

do not spray anything to avoid over-wetting the substrate; when transplanting, try to

preserve the substrate on the root system; when potting, do not compact the

substrate, water thoroughly in time to ensure that the cuttings are in close contact

with the soil to recover the seedlings; two days after transplanting, water again, then carry out routine water management, fertilize for the first time after one month, and then spray the nutrient solution once every ten days, The nutrient solution formula is

0.1% urea by mass percentage and potassium dihydrogen phosphate with 0.3% mass

percentage. The spraying time of the nutrient solution is to spray with a sprayer after

water spraying, and the dosage is 2L/m2 . Observe the growth of transplanted

seedlings, and it is observed that the survival rate of transplanted seedlings is 90%

after three months.

Embodiment 4

The method for cutting and planting Sanguisorba officinalis includes the following

steps:

(1) Selection of a nursery land: choose a place with open terrain, deep soil layer and

fertile soil as the nursery land for cutting and planting.

(2) Preparation of a planting bed: the planting bed is surrounded by cement in an

arch shed with double-layer films covered with plastic film, rectangular in east-west

direction, leeward, facing sun and enjoying sufficient sunlight. An upper part of the

planting bed is shaded by 80% shading net, the planting bed is 40cm above the

ground, and the planting bed is equipped with artificial spraying facilities. The

planting bed has a thickness of 50cm, and the planting bed consists of three layers.

An upper layer is river sand with a thickness of 14cm, which has good air

permeability and a good water retention property. River sand used has been exposed

in sunlight for two days, which has a certain sterilization effect; under the river sand

is coarse sand with a thickness of 20cm, and the bottom layer is covered with

pebbles with a thickness of 15cm to increase water permeability. Treat the planting

bed (including river sand, coarse sand and pebbles) with 700mg/kg potassium

permanganate solution one week before planting, and rinse thoroughly with water

after three days of treatment.

(3) Preparation of cuttings: In August of each year, select robust, disease-free, and lignified Sanguisorba officinalis plants, and use sharp scissors to cut the lignified ground stems with a diameter of 0.4cm or stems with pinnate leaves with a diameter of about 0.3cm in the early morning or evening. Put the stems immediately in a bucket filled with distilled water. Among them, the scissors and the bucket have been sprayed and disinfected with 75% alcohol.

The stems after cutting should be processed in time, to remove the tender parts at

spikes, and cut them into short cuttings with a length of 10cm. And then upper ends

of the cuttings are flat and 1 cm away from the buds, with one to two small leaves,

and when the leaves are large, reducing 1/2 or 1/3; lower ends thereof are a

horse-ear-shaped oblique end with one to two young buds. Bundle the prepared

cuttings in a neat manner with the flat parts on the top and the oblique parts on the

bottom. Each fifty roots are tied into a bundle, and the upper and lower ends of the

cuttings are disinfected by adding 0.3% carbendazim (500 times solution). After

soaking for two minutes, wash with distilled water twice. Soak the cleaned lower end

of the cuttings with 100mg/L ABT2 rooting powder (purchased from Beijing Aibiti

Biotechnology Co., Ltd.) for two min, with soaking depth 1-3cm, and the ABT rooting

powder is prepared with a 50% alcohol solution by volume.

(4) Planting: when planting, use wooden sticks to draw holes or slots 2-4cm deep,

planting depth 3cm, plant spacing 4cm, row spacing 5cm, cuttings inclination 75,

bury the cuttings after cutting and compact the surrounding substrate with both

hands. Keep the cuttings in close contact with the substrate, and water the cuttings

once immediately after the cuttings are finished.

(5) Post-planting management: after planting, weeds should be timely weeded on

cloudy and rainy days, and attention should be paid to disease prevention and pest

control. In the first five days after planting, pouring water twice a day, in the early

morning and evening respectively, and cover with plastic film to make the light

transmittance in the shed 85%. Fully automatic spraying device is used to spray five

to six times a day to keep the relative air humidity at 90% and the air temperature at

-30C. When the temperature in the shed is too high, open two ends of the plastic film to ventilate and cool down. When the light is too strong at noon, spread the shade net and the plastic film. Fifteen days after planting, appropriately reduce the number of spraying to three to four times a day, and the relative air humidity is controlled at 65%. When the callus growth or rooting begins, spray 0.2% urea water solution to supply the nutrients needed by the plants. Spray thiophanate-methyl (500 times solution) every ten days to prevent cuttings from going moldy, check temperature and humidity every day, and remove rotten cuttings in time. In order to prevent the base of the cuttings from rotting, spray 500 times carbendazim through the substrate every seven days after planting. The spraying time is in the evening after water is sprayed. Observe the growth of the cuttings at any time.

(6) Transplanting after planting: one month after planting, the root length is 3-5cm,

the rooting rate is 90%, the root system is well developed, pull out the cuttings in the

early morning or evening and the survival rate is 85%. Transplant the cuttings in pots

or fields within one day after pulling out. The day before transplanting, do not spray

anything to avoid over-wetting the substrate; when transplanting, try to preserve the

substrate on the root system; when potting, do not compact the substrate, water

thoroughly in time to ensure that the cuttings are in close contact with the soil to

recover the seedlings; two days after transplanting, water again, then carry out

routine water management, fertilize for the first time after one month, and then

spray the nutrient solution once every seven days, The nutrient solution formula is

0.05% urea by mass percentage and potassium dihydrogen phosphate with 0.3%

mass percentage. The spraying time of the nutrient solution is to spray with a sprayer

after water spraying, and the dosage is 1.5L/m 2 . Observe the growth of transplanted

seedlings, and it is observed that the survival rate of transplanted seedlings is 96%

after three months.

The above are only preferred embodiments of the present invention, and are not

intended to limit the present invention. Any modifications, equivalent replacement

and improvement made within the spirits and principles of the present invention

shall be included in the protection scope of the present invention.

Claims (10)

CLAIMS:

1. A method for cutting and planting Sanguisorba officinalis, characterized in that the

method including following steps:

1) Preparation of a planting bed: arranging sterilized substrates in the planting bed,

the substrate including river sand, coarse sand and pebbles;

2) Preparation of cuttings: cutting lignified stem segments as cuttings from June to

September, cutting the cuttings into sections with a length of 5-12 cm, and then

disinfecting the cutting sections, cleaning and giving hormone treatment;

3) Planting: digging holes or slots in the substrate, inserting the hormone-treated

cuttings into the holes or the channels at an inclination of 75, and pouring water

thoroughly after planting;

4) Management after planting: after planting, giving weeding and pest control

treatments within one to five days after planting, pouring water in the morning and

evening every day, meanwhile, maintaining light transmittance of 80-90%, relative air

humidity of 70-90%, and temperature of 18-38°C; fifteen days after planting, keeping

relative air humidity at 60-70%, and when damaged tissue recovery or rooting begins,

spraying urea water solution with a mass percentage 0.2%; and

) Transplanting: when the root length is 3-5cm, transplanting with the substrate

remaining on the root system, watering thoroughly after transplanting, and spraying

nutrient solution every seven to ten days after one month.

2. The method for cutting and planting Sanguisorba officinalis according to claim 1,

wherein in the step 1), the substrate has three layers, and an upper layer is river sand

with a thickness of 10-15 cm, an intermediate layer is coarse sand with a thickness of

cm, and a lower layer is pebbles with a thickness of 15 cm.

3. The method for cutting and planting Sanguisorba officinalis according to claim 1,

wherein in the step 1), a method of sterilizing is to expose and disinfect river sand for

two days, and one week prior to planting, use 400-800mg/kg potassium

permanganate solution to disinfect river sand, coarse sand and pebbles for three days, and then rinse with water.

4. The method for cutting and planting Sanguisorba officinalis according to claim 1,

wherein in the step 2), the stem segments are ground stems or stem segments with

pinnate leaves, and thickness of the stem segments is 0.2 - 0.5 cm.

5. The method for cutting and planting Sanguisorba officinalis according to claim 1,

wherein in the step 2), putting the cuttings immediately into water after cutting.

6. The method for cutting and planting Sanguisorba officinalis according to claim 1,

wherein in the step 2), an upper end of the cuttings is flat and 1 cm away from the

buds, with one to two small leaves, and when the leaves are large, reducing 1/2 or

1/3; a lower end thereof is a horse-ear-shaped oblique end with one to two young

buds.

7. The method for cutting and planting Sanguisorba officinalis according to claim 1,

wherein in the step 2), a method of disinfecting is to soak upper ends and lower ends

of the cuttings with 500 times carbendazim liquid 0.3% for one to three minutes.

8. The method for cutting and planting Sanguisorba officinalis according to claim 1,

wherein in the step 2), the hormone treatment method is to soak the cleaned lower

ends of the cuttings with 50-300mg/L rooting powder for one to three minutes.

9. The method for cutting and planting Sanguisorba officinalis according to claim 1,

wherein in the step 4), the pest control method is spraying cuttings with 500 times

thiophanate methyl every eight to twelve days, and spraying the substrate with 500

times carbendazim every five to nine days.

10. The method for cutting and planting Sanguisorba officinalis according to claim 1,

wherein in the step 5), the nutrient solution contains urea with a mass percentage of

-0.1% and potassium dihydrogen phosphate with a mass percentage of 0.1-0.5%,

and a dosage thereof is 1-2L/m 2

.