AU2017382367B2 - Binding polypeptides and methods of making the same - Google Patents

Binding polypeptides and methods of making the same Download PDF

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Publication number
AU2017382367B2
AU2017382367B2 AU2017382367A AU2017382367A AU2017382367B2 AU 2017382367 B2 AU2017382367 B2 AU 2017382367B2 AU 2017382367 A AU2017382367 A AU 2017382367A AU 2017382367 A AU2017382367 A AU 2017382367A AU 2017382367 B2 AU2017382367 B2 AU 2017382367B2
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Australia
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cell
strand
sequence
cdna
nucleic acid
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AU2017382367A
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AU2017382367A1 (en
Inventor
Gregory Babcock
Luke Robinson
Zachary Shriver
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Visterra Inc
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Visterra Inc
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/005Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/60Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622Single chain antibody (scFv)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2017382367A 2016-12-23 2017-12-22 Binding polypeptides and methods of making the same Active AU2017382367B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662438712P 2016-12-23 2016-12-23
US62/438,712 2016-12-23
PCT/US2017/068204 WO2018119402A1 (en) 2016-12-23 2017-12-22 Binding polypeptides and methods of making the same

Publications (2)

Publication Number Publication Date
AU2017382367A1 AU2017382367A1 (en) 2019-06-13
AU2017382367B2 true AU2017382367B2 (en) 2024-11-21

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AU2017382367A Active AU2017382367B2 (en) 2016-12-23 2017-12-22 Binding polypeptides and methods of making the same

Country Status (15)

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US (2) US11820979B2 (https=)
EP (1) EP3559028A1 (https=)
JP (2) JP7360324B2 (https=)
KR (2) KR102702552B1 (https=)
CN (1) CN110366557B (https=)
AU (1) AU2017382367B2 (https=)
BR (1) BR112019013096A2 (https=)
CA (1) CA3045696A1 (https=)
CL (2) CL2019001757A1 (https=)
CO (1) CO2019006663A2 (https=)
IL (2) IL315727A (https=)
MX (1) MX2019007356A (https=)
MY (1) MY201744A (https=)
RU (1) RU2019123063A (https=)
WO (1) WO2018119402A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3781677A4 (en) * 2018-04-16 2022-01-19 University of Massachusetts COMPOSITIONS AND METHODS FOR IMPROVED GENE EDITTING
CN109161572B (zh) * 2018-07-05 2021-11-12 中国海洋大学 单链环状核酸及其制备方法和应用
WO2020097395A1 (en) 2018-11-08 2020-05-14 The Regents Of The University Of California Systems and methods for targeting cancer cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010022738A1 (en) * 2008-08-29 2010-03-04 Symphogen A/S Method for cloning avian-derived antibodies
WO2013092716A1 (en) * 2011-12-21 2013-06-27 F. Hoffmann-La Roche Ag Rapid method for cloning and expression of cognate antibody variable region gene segments
WO2015166272A2 (en) * 2014-05-02 2015-11-05 Iontas Limited Preparation of libraries of protein variants expressed in eukaryotic cells and use for selecting binding molecules

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
JPS6147500A (ja) 1984-08-15 1986-03-07 Res Dev Corp Of Japan キメラモノクロ−ナル抗体及びその製造法
EP0173494A3 (en) 1984-08-27 1987-11-25 The Board Of Trustees Of The Leland Stanford Junior University Chimeric receptors by dna splicing and expression
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
JPS61134325A (ja) 1984-12-04 1986-06-21 Teijin Ltd ハイブリツド抗体遺伝子の発現方法
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
JP3101690B2 (ja) 1987-03-18 2000-10-23 エス・ビィ・2・インコーポレイテッド 変性抗体の、または変性抗体に関する改良
EP0436597B1 (en) 1988-09-02 1997-04-02 Protein Engineering Corporation Generation and selection of recombinant varied binding proteins
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
GB8905669D0 (en) 1989-03-13 1989-04-26 Celltech Ltd Modified antibodies
WO1991000906A1 (en) 1989-07-12 1991-01-24 Genetics Institute, Inc. Chimeric and transgenic animals capable of producing human antibodies
DE69120146T2 (de) 1990-01-12 1996-12-12 Cell Genesys Inc Erzeugung xenogener antikörper
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
DK0585287T3 (da) 1990-07-10 2000-04-17 Cambridge Antibody Tech Fremgangsmåde til fremstilling af specifikke bindingsparelementer
ATE158021T1 (de) 1990-08-29 1997-09-15 Genpharm Int Produktion und nützung nicht-menschliche transgentiere zur produktion heterologe antikörper
DE69133557D1 (de) 1990-08-29 2007-03-15 Pharming Intellectual Pty Bv Homologe rekombination in säugetier-zellen
CA2095633C (en) 1990-12-03 2003-02-04 Lisa J. Garrard Enrichment method for variant proteins with altered binding properties
EP1820858B1 (en) 1991-03-01 2009-08-12 Dyax Corporation Chimeric protein comprising micro-protein having two or more disulfide bonds and embodiments thereof
ATE414768T1 (de) 1991-04-10 2008-12-15 Scripps Research Inst Bibliotheken heterodimerer rezeptoren mittels phagemiden
EP0519596B1 (en) 1991-05-17 2005-02-23 Merck & Co. Inc. A method for reducing the immunogenicity of antibody variable domains
DE4122599C2 (de) 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
DK1621554T4 (da) 1992-08-21 2012-12-17 Univ Bruxelles Immunoglobuliner blottet for lette kæder
US5851804A (en) 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
CA2258570A1 (en) 1996-06-17 1997-12-24 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs
US20020086330A1 (en) 2000-01-31 2002-07-04 Rosen Craig A. Nucleic acids, proteins, and antibodies
EP1259601A2 (en) 2000-02-22 2002-11-27 Ahuva Nissim Chimeric and tcr phage display libraries, chimeric and tcr reagents and methods of use thereof
HRP20080197T8 (hr) * 2003-09-18 2008-07-31 Symphogen A/S Postupak za povezivanje sekvenci od interesa
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
US20060199191A1 (en) 2004-12-13 2006-09-07 Demian Obregon Methods and compositions for the synthesis of RNA and DNA
CN101370832B (zh) 2005-12-02 2014-07-02 健泰科生物技术公司 结合多肽及其用途
BRPI0808551B1 (pt) * 2007-03-01 2022-04-05 Symphogen A/S Composições de anticorpo para o receptor do fator de crescimento antiepidérmico recombinante, molécula de ligação bi-específica e composição farmacêutica que os compreende
DK2121920T3 (da) 2007-03-01 2011-11-21 Symphogen As Fremgangsmåde til kloning af sammenhørende antistoffer
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
EP3124497B1 (en) 2007-09-14 2020-04-15 Adimab, LLC Rationally designed, synthetic antibody libraries and uses therefor
EP2604279A1 (en) 2008-03-27 2013-06-19 ZymoGenetics, Inc. Compositions and methods for inhibiting PDGFRBETA and VEGF-A
WO2010005593A1 (en) * 2008-07-11 2010-01-14 President And Fellows Of Harvard College Systems and methods of droplet-based selection
US8497071B2 (en) 2009-06-29 2013-07-30 California Institute Of Technology Isolation of unknown rearranged T-cell receptors from single cells
IT1395574B1 (it) 2009-09-14 2012-10-16 Guala Dispensing Spa Dispositivo di erogazione
WO2011071943A1 (en) * 2009-12-07 2011-06-16 Prognosys Biosciences, Inc. Peptide display arrays
CA2799746C (en) 2010-05-17 2020-11-24 Sai Reddy Rapid isolation of monoclonal antibodies from animals
EP2640745B1 (en) * 2010-09-10 2018-11-07 MedImmune Limited Bivalent and bispecific anti-il6/anti-il23 antibodies
WO2012083225A2 (en) 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
CN103732738A (zh) 2011-04-28 2014-04-16 小利兰斯坦福大学托管委员会 与样品相关的多核苷酸的鉴定
US20140243234A1 (en) 2011-08-03 2014-08-28 Texas Biomedical Research Institute Nucleic acid compositions, methods and kits for rapid pairing of affinity agents
EP2783214A2 (en) 2011-11-23 2014-10-01 The Board of Regents of The University of Texas System Proteomic identification of antibodies
US9701959B2 (en) 2012-02-02 2017-07-11 Invenra Inc. High throughput screen for biologically active polypeptides
EP2626433B1 (en) 2012-02-09 2017-04-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for linking nucleic acids in a microsome from endoplasmatic reticuli
SG11201408128WA (en) 2012-06-15 2015-01-29 Adaptive Biotechnologies Corp Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
EP2861760B1 (en) * 2012-06-15 2020-01-01 The Board of Regents of The University of Texas System High throughput sequencing of multiple transcripts of a single cell
WO2014043813A1 (en) 2012-09-19 2014-03-27 British Columbia Cancer Agency Branch Immune repertoire profiling
GB2525568B (en) 2013-03-15 2020-10-14 Abvitro Llc Single cell barcoding for antibody discovery
KR20150132479A (ko) 2013-03-15 2015-11-25 어댑티브 바이오테크놀로지스 코포레이션 복합 유전자 세트에서의 독특하게 태그되고 재배열된 적응 면역 수용체 유전자
RU2578009C2 (ru) 2013-05-08 2016-03-20 Закрытое акционерное общество "ЕВРОГЕН" Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках
EP3041934A1 (en) * 2013-09-03 2016-07-13 Moderna Therapeutics, Inc. Chimeric polynucleotides
GB201402591D0 (en) 2014-02-14 2014-04-02 Memo Therapeutics Ag Method for recovering two or more genes, or gene products, encoding an immunoreceptor
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US10202640B2 (en) 2014-05-07 2019-02-12 The Board Of Trustees Of The Leland Stanford Junior University Single cell analysis of T cells using high-throughput multiplex amplification and deep sequencing
EP3517625B1 (en) 2014-05-22 2021-01-20 The University of British Columbia Methods for determining lymphocyte receptor chain pairs
EP3950944A1 (en) 2014-09-15 2022-02-09 AbVitro LLC High-throughput nucleotide library sequencing
CA2966201A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10513733B2 (en) 2015-03-23 2019-12-24 Board Of Regents, The University Of Texas System High throughout sequencing of paired VH and VL transcripts from B cells secreting antigen-specific antibodies
AU2016253964B2 (en) 2015-04-27 2022-07-07 Abvitro Llc Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigens
US9422547B1 (en) 2015-06-09 2016-08-23 Gigagen, Inc. Recombinant fusion proteins and libraries from immune cell repertoires
EP3443087B1 (en) * 2016-04-12 2024-09-18 Medimmune, LLC Immune repertoire mining

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010022738A1 (en) * 2008-08-29 2010-03-04 Symphogen A/S Method for cloning avian-derived antibodies
WO2013092716A1 (en) * 2011-12-21 2013-06-27 F. Hoffmann-La Roche Ag Rapid method for cloning and expression of cognate antibody variable region gene segments
WO2015166272A2 (en) * 2014-05-02 2015-11-05 Iontas Limited Preparation of libraries of protein variants expressed in eukaryotic cells and use for selecting binding molecules

Also Published As

Publication number Publication date
JP7360324B2 (ja) 2023-10-12
CN110366557A (zh) 2019-10-22
US11820979B2 (en) 2023-11-21
WO2018119402A1 (en) 2018-06-28
JP2023169422A (ja) 2023-11-29
KR20240133790A (ko) 2024-09-04
CO2019006663A2 (es) 2019-06-28
CA3045696A1 (en) 2018-06-28
IL267565A (en) 2019-08-29
US20180201926A1 (en) 2018-07-19
CN110366557B (zh) 2024-04-09
KR102702552B1 (ko) 2024-09-03
MY201744A (en) 2024-03-15
JP2020511117A (ja) 2020-04-16
RU2019123063A (ru) 2021-01-26
CL2019001757A1 (es) 2019-11-29
KR20190111036A (ko) 2019-10-01
IL267565B1 (en) 2024-10-01
US20240141334A1 (en) 2024-05-02
RU2019123063A3 (https=) 2021-11-29
MX2019007356A (es) 2019-09-05
IL267565B2 (en) 2025-02-01
AU2017382367A1 (en) 2019-06-13
EP3559028A1 (en) 2019-10-30
IL315727A (en) 2024-11-01
BR112019013096A2 (pt) 2019-12-17
CL2022000454A1 (es) 2023-03-24

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