NZ795976A - Binding polypeptides and methods of making the same - Google Patents
Binding polypeptides and methods of making the sameInfo
- Publication number
- NZ795976A NZ795976A NZ795976A NZ79597617A NZ795976A NZ 795976 A NZ795976 A NZ 795976A NZ 795976 A NZ795976 A NZ 795976A NZ 79597617 A NZ79597617 A NZ 79597617A NZ 795976 A NZ795976 A NZ 795976A
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Abstract
Polypeptides, such as antibody molecules and TCR molecules, and methods of making the same, are disclosed. The polypeptides can be used to treat, prevent, and/or diagnose disorders.
Description
Polypeptides, such as antibody les and TCR molecules, and methods of making the same,
are disclosed. The polypeptides can be used to treat, prevent, and/or diagnose disorders.
NZ 795976
BINDING POLYPEPTIDES AND METHODS OF MAKING THE SAME
This is a onal ofNew Zealand patent application No. 753978, the entire contents
of which are incorporated herein by reference.
BACKGROUND
Monoclonal antibody therapies are a class of irnmunotherapies that involve monoclonal
antibodies (mAbs) that are capable of specifically cting with e-relevant biological
molecules. In recent years, the disease areas that therapeutic antibodies can target have significantly
expanded, and a number of monoclonal antibodies and antibody-derivative products have been
ed for therapeutic use in the United States and many other countries. Monoclonal antibody
therapies are currently used or investigated for treating various diseases or conditions, including, for
example, infectious diseases, cancer, immune diseases, organ transplantation, cardiovascular diseases,
and metabolic diseases.
Given the y of monoclonal antibodies and dy-derivative ts in modulating
various biological functions, the need exists for developing new approaches for generation of
antibodies le for treating, preventing, and diagnosing disorders.
SUMMARY
This disclosure provides, at least in part, binding polypeptides (e.g., antibody molecules or T-
cell receptor (TCR) molecules) that comprise one or more of the structural or functional properties
disclosed herein. In an embodiment, libraries of the binding polypeptides, methods for making the
polypeptides or libraries, nucleic acid molecules encoding the binding polypeptides, expression
vectors, host cells, compositions (e.g., ceutical compositions), kits, and containers, are also
ed. The ptides (e.g., antibody molecules or TCR molecules) disclosed herein can be
used (alone or in combination with other agents or therapeutic modalities) to treat, prevent and/or
diagnose disorders, such as disorders and conditions disclosed herein.
In an aspect, the disclosure features a method of making a nucleic acid sequence comprising a
sequence that encodes a heavy chain element (HC t) of an antibody heavy chain variable
region (HCVR) and a light chain t (LC element) of an antibody light chain variable region
(LCVR), and wherein the HCVR and LCVR are matched, the method comprising:
a) acquiring an ed tion reaction site, e.g., a production micro-chamber,
comprising:
i) a heavy chain (HC) strand, wherein the HC strand is a strand of a heavy chain -
ed cDNA (HC ds cDNA) comprising a segment that encodes an HC element of the HCVR from
a cell, e. g., a heavy chain le region sequence (HCVRS); and
ii) a light chain (LC) strand, n the LC strand is a strand of a light chain double-stranded
cDNA (LC ds cDNA) comprising a segment that encodes an LC element of the LCVR from the cell,
e.g., a light chain variable region sequence (LCVRS), and
b) covalent linking, e. g., ligation, of an HC strand to an LC strand,
wherein the isolated production reaction site, e.g., a tion micro-chamber, does not
e a nucleic acid encoding an HCVR or an LCVR from a cell other than the cell (e. g., a different
cell, e.g., a different B cell),
thereby making a nucleic acid sequence comprising a sequence that encodes an HC element
of an HCVR and a LC element of an LCVR, wherein the HCVR and LCVR are matched.
In an embodiment, the HC element comprises, or consists of, an HCVRS, or a onal
nt thereof (e.g., an antigen binding fragment thereof). In an embodiment, the LC element
comprises, or ts of, an LCVRS, or a functional fragment thereof (e.g., an antigen binding
fragment thereof).
In an embodiment, the HC ds cDNA comprises a segment that encodes an HCVRS. In an
embodiment, the LC ds cDNA comprises a segment that encodes an LCVRS. In an embodiment, the
HC ds cDNA comprises a t that s an HCVRS, and the LC ds cDNA comprises a
segment that encodes an LCVRS.
In an embodiment, the cell is an immune cell, e.g., a B cell, e.g., a human B cell. In an
embodiment, the cell is a mammalian cell or an avian cell.
In an ment, the nucleic acid sequence is configured such that, when expressed, the HC
element and the LC element (6.3., the HCVRS and the LCVRS) form a functional antigen binding
molecule, e.g., an scFv, an Fab, or an scFab. In an embodiment, the antigen binding molecule, e.g.,
an scFv, is functional in vitro, ex vivo, or in vivo, e.g., as determined by a method or assay described
herein.
In an embodiment, acquiring an isolated production reaction site, e.g., a production micro-
chamber, comprises:
a) ing a capture substrate bound to: (i) a first double-stranded cDNA (ds cDNA)
comprising a strand that is mentary to a first mRNA that encodes an HCVR from a cell; and
(ii) a second ds cDNA comprising a strand complementary to a second mRNA encoding an LCVR
from the cell (the cDNA loaded capture substrate), and
b) maintaining the isolated production reaction site, e.g., the production micro-chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce: a plurality of
HC ds cDNAs comprising a segment that encodes an HC element of the HCVR from the cell, e.g., an
HCVRS; and a plurality of LC ds cDNAs comprising a segment that encodes an LC element of the
LCVR from the cell, e. g., an LCVRS.
In an embodiment, the HC ds cDNA is identical, or substantially identical, to the first ds
cDNA. For example, the sense strand of the HC ds cDNA is at least 80%, 85 %, 90%, 95%, 98%,
99%, or 100% cal to, or differs by no more than 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50
nucleotides from, the sense strand of the first ds cDNA, and/or the antisense strand of the HC ds
cDNA is at least 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to, or differs by no more than 1,
2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50 nucleotides from, the antisense strand of the first ds cDNA.
In an embodiment, the LC ds cDNA is identical, or substantially identical, to the second ds
cDNA. For example, the sense strand of the LC ds cDNA is at least 80%, 85%, 90%, 95%, 98%,
99%, or 100% identical to, or differs by no more than 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50
nucleotides from, the sense strand of the second ds cDNA, and/or the antisense strand of the LC ds
cDNA is at least 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to, or differs by no more than 1,
2, 5, 10, 15, 20, 25, 30, 35, 40, 45 , or 50 tides from, the antisense strand of the second ds
cDNA.
In an embodiment, the HC strand is a sense strand. In an embodiment, the LC strand is a
sense strand. In an embodiment, the HC strand is an antisense strand. In an embodiment, the LC
strand is an antisense strand. In an embodiment, both the HC strand and the LC strand are sense
strands. In an embodiment, both the HC strand and the LC strand are antisense strands.
In an embodiment, the capture substrate comprises a bead, e.g., a magnetic bead. In an
embodiment, the capture substrate comprises a moiety (e.g., an oligonucleotide) which binds to
cDNA, e.g., (i) a moiety which binds to the HC strand; (ii) a moiety which binds to the LC ; or
(iii) both (i) and (ii). In an embodiment, the moiety which binds to the HC strand is different from the
moiety which binds to the LC strand, e.g., to facilitate creating conditions favorable to ing
similar levels of each DNA le type. In an embodiment, the moiety which binds to the HC
strand is identical to the moiety which binds to the LC strand.
In an embodiment, the first mRNA and the second mRNA are disposed on an mRNA loaded
capture substrate.
In an embodiment, the isolated production reaction site, e.g., the production micro-chamber,
comprises: a reagent mixture suitable for producing, from the first and second mRNAs (e.g., after the
first and second mRNAs are released from the mRNA loaded capture substrate into a solution), a first
ds cDNA comprising a segment that encodes an HC element of the HCVR of the cell, e.g., an
HCVRS, and a second ds cDNA comprising a segment that s an LC element of the LCVR of
the cell, e.g., an LCVRS.
In an embodiment, the isolated production on site, e.g., tion micro-chamber,
comprises primers that mediate the production of the first ds cDNA. In an embodiment, the isolated
production on site, e.g., production micro-chamber, comprises primers that mediate the
production of the second ds cDNA.
In an embodiment, a cDNA strand that is complementary to a first mRNA that encodes an
HCVR from a cell is made by reverse transcription of the first mRNA. In an embodiment, a cDNA
strand that is mentary to a second mRNA that encodes an LCVR from a cell is made by
reverse transcription of the second mRNA.
In an embodiment, the reverse transcription takes place in the isolated production reaction
site, e.g., a production-micro chamber. In an ment, the reverse transcription takes place in an
isolated cell reaction site, e.g., a cell isolation micro-chamber. In an ment, the reverse
transcription takes place outside the isolated production reaction site, e.g., a production micro-
chamber, or outside an isolated cell reaction site, e.g., a cell isolation micro-chamber. In an
embodiment, the reverse transcription takes place outside the ed production reaction site, e.g., a
production-micro chamber, and outside an isolated cell reaction site, e.g., a cell isolation micro-
chamber. In an embodiment, the reverse transcription takes place outside an isolated reaction site,
e.g., outside a chamber.
In an embodiment, the amplification comprises 30 or fewer cycles, e.g., 20 or fewer cycles,
e.g., 15 or fewer, 14 or fewer, 13 or fewer, 12 or fewer, 11 or fewer, 10 or fewer, 9 or fewer, 8 or
fewer, 7 or fewer, 6 or fewer, or 5 or fewer cycles.
In an embodiment, the reverse transcription and/or amplification uses one or more primers,
e.g., comprising a sequence specific for an HCVRS and/or an LCVRS.
In an embodiment, the reverse transcription and/or amplification comprises using two or more
primers that mediate the production of the HC ds cDNA, wherein at least one primer comprises a
nucleotide modification, and wherein at least one primer does not comprise a nucleotide modification.
In an embodiment, the amplification ses using two or more primers that mediate the tion
of the LC ds cDNA, wherein at least one primer ses a nucleotide modification, and wherein at
least one primer does not comprise a nucleotide modification.
In an embodiment, at least one primer comprises a tide modification, e.g., which
reduces, e.g., inhibits, DNA synthesis, e.g., by a DNA polymerase. In an embodiment, at least one
primer does not comprise a nucleotide modification, e.g., which reduces, e.g., inhibits, DNA
synthesis, e.g., by a DNA polymerase.
In an embodiment, the nucleotide modification inhibits a DNA rase from extending
the DNA. Without wishing to be bound by theory, it is believed that in an embodiment, any chemical
entity that reduces (e.3., blocks) DNA polymerase extension can be used in accordance with the
methods described herein.
In an ment, the nucleotide modification is an insertion of a spacer to the , e. g.,
between two nt nucleotides in the primer. In an embodiment, the spacer is a flexible spacer. In
an embodiment, the spacer is a carbon spacer (e.g., n-, wherein n=3, 4, 5, 6, 7, 8, 9, 10, or
more), two or more (e.g., three, four, five, six, seven, eight, nine, ten, or more) abasic nucleotides, or a
hylene glycol (PEG) spacer. In an embodiment, the spacer is a PEG . In an embodiment,
the tide modification is 2’-O-methyl, 2’-OH, 2’-NH2, or uracil, e.g., to a ribose.
In an embodiment, the nucleotide modification is located internally or at the 3’ end of the
. In an embodiment, at least one primer comprises (i) a first member; (ii) a second member;
and optionally (iii) a third member, e.g., comprising a nucleotide modification described herein, e.g.,
d between (i) and (ii).
In an embodiment, the first member is capable of annealing with the second member. In an
embodiment, the first member is capable of annealing with the second member in the same primer,
e.g., through intra-molecular hybridization, e.g., to form a hairpin structure sing a duplex
region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more basepairs. In another
embodiment, the first member is capable of annealing hybridizing with the second member in a
different primer, e.g., through inter-molecular hybridization, e.g., to form a double-stranded structure
sing a duplex region of 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more
basepairs. Without wishing to be bound by theory, it is believed that in an ment, there are at
least two secondary structures that the modified primers can form and facilitate reduction (e.g.,
prevention) of competition to substrate (e.g., bead) capture. For example, the secondary structure can
be a hairpin-like structure formed by intra-molecular hybridization (within the same primer), or the
secondary structure can be a duplex structure formed by molecular hybridization (between two
different s).
In an embodiment, the first member comprises a sequence that is mentary to the
sequence of an ucleotide attached to the capture substrate. In an embodiment, the second
member comprises (e.g., from 5’ to 3’) one, two, or all of: (i) a sequence that is mentary to at
least a portion of the first member; (ii) a universal priming sequence (e.g., for PCR amplification or
next-generation sequencing); and (iii) a sequence complementary to a target sequence, e.g., an
HCVRS and/or an LCVRS. In an embodiment, the universal priming sequence is identical, or
substantially identical, to the sequence that is complementary to at least a portion of the first member.
In another embodiment, the universal priming ce is ent from the sequence that is
complementary to at least a portion of the first member. In an embodiment, the second member
comprises a sequence for homologous recombination (e.g., in a yeast or mammalian cell).
In an embodiment, at least one primer comprises a sequence encoding at least a portion of a
linker sequence, or a complementary sequence thereof. In an ment, the primer that ses
a sequence encoding at least a portion of a linker sequence, or a complementary sequence f, is
phosphorylated, e.g., 5’ phosphorylated. Without wishing to be bound by theory, it is believed that in
an embodiment, any sequence with the general properties of flexibility (e.g., facilitated by glycine)
and hydrophilicity can work effectively in accordance with the methods described herein. Exemplary
linkers can generally have overrepresentation of one or more of Gly, Ser, Thr, or Ala and
WO 19402
underrepresentation of hydrophobic residues, e.g., one or more of Trp, Tyr, Phe, Cys, Met, Leu, or Ile.
The length of the primer may vary, e.g., 3-50 amino acid es (e.g., 5-45, 10-40, 15-35, 20-30, 10-
, 10-30, 20-40, or 30-40 amino acid residues). In an embodiment, the linker sequence comprises, or
consists of, ((Gly)m-Ser))n, where m=3, 4, 5, or more and n=1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more. In an
embodiment, the linker sequence comprises, or consists of, (Gly-Gly-Gly-Gly-Ser)n, where n=1, 2, 3,
4, 5, 6, 7, 8, 9, 10, or more.
In an embodiment, the primer is a primer described herein, e.g., in Examples.
In an embodiment, the reverse transcription, the amplification, or both, occurs in a solution in
the isolated production on site, e.g., production micro-chamber. In an embodiment, the reverse
transcription, the amplification, or both, does not occur on the substrate (e.g., bead). For example, the
reverse transcription, the amplification, or both, can occur on in a solution within a droplet.
In an embodiment, the HC ds cDNA comprises a 5’ overhang, e.g., a 5’ overhang that is
capable of hybridizing to an oligonucleotide attached to a capture substrate. In an embodiment, the
HC ds cDNA comprises a blunt end, e.g., a blunt end comprising a 5’ phosphate. In an embodiment,
the LC ds cDNA comprises a 5’ overhang, e.g., a 5’ overhang that is capable of izing to an
oligonucleotide attached to a capture substrate. In an embodiment, the LC ds cDNA comprises a
blunt end, e.g., a blunt end comprising a 5’ phosphate. In an ment, the HC ds cDNA and the
LC ds cDNA comprise sticky ends, e.g., both have 5 ’ overhangs.
In an embodiment, the HC strand and the LC strand are covalently linked, e.g., ligated, to
produce a single stranded nucleic acid sequence, wherein the HC and LC s are both sense
s or both antisense s. In an ment, a denatured HC strand of the HC ds cDNA to a
denatured LC strand of the LC ds cDNA are covalently , e.g., ligated, wherein the HC and LC
strands are both sense strands or both antisense strands. In an embodiment, the HC strand is present
in the HC ds cDNA and the LC strand is present in the LC ds cDNA, and wherein the HC ds cDNA
and the LC ds cDNA are covalently linked, e.g., ligated, e.g., to produce a double stranded nucleic
acid sequence.
In an embodiment, the covalent linking, e. g., on, occurs in the ed production
reaction site. In an embodiment, the isolated production reaction site, e.g., a production micro-
chamber, or the isolated linkage reaction site, e.g., a linkage micro-chamber, comprises a reagent that
is e of covalently linking, e.g., ligating, the HC and LC strands or the HC and LC ds cDNAs.
In an embodiment, the isolated production reaction site, e.g., a production micro-chamber ses
an enzyme that covalently couples the HC and LC strands or the HC and LC ds cDNAs. In an
embodiment, the enzyme is a ligase, e. g., a thermal stable ligase. In an embodiment, the covalent
linking comprises ligase thermocycling.
In an embodiment, the covalent linking, e. g., ligation, occurs in a site different from the
isolated production reaction site, e.g., occurs in an isolated e reaction site, e.g., a linkage micro-
chamber. In an embodiment, the HC strand and the LC strand are transferred from the isolated
production site to the ed linkage reaction site, e.g., a linkage micro-chamber, and the covalent
linking occurs in the isolated linkage reaction site, e.g., a linkage micro-chamber. In an embodiment,
the isolated linkage on site, e.g., a linkage micro-chamber, comprises a reagent that is capable of
covalently linking, e.g., ligating, the HC and LC strands or the HC and LC ds cDNAs. In an
embodiment, the ed linkage on site, e. g., a linkage micro-chamber, comprises an enzyme
that covalently s the HC and LC s or the HC and LC ds cDNAs. In an embodiment, the
enzyme is a , e.g., a thermal stable ligase. In an embodiment, the covalent linking comprises
ligase thermocycling.
In an embodiment, the covalent linking, e.g., ligation, comprises: (a) heating the isolated
linkage reaction site, e.g., the linkage micro-chamber, under conditions (e.g., at 950C) that allow
denaturation of the HC strand and the LC strand; (b) cooling the isolated linkage reaction site, e.g., the
linkage micro-chamber, under conditions (e.g., at 50-65°C) that allow hybridization of the splint
oligonucleotide to the HC strand and the LC strand; (c) maintaining the isolated linkage reaction site,
e. g., the linkage micro-chamber, under ions (e.g., at 45-65°C) that allow ligation of the HC
strand and the LC strand (e.g., formation of phosphodiester bond between the HC strand and the LC
strand); and (d) repeating steps (a), (b), and (c) sequentially for 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50,
or more cycles.
In an embodiment, the HC strand and the LC strand are covalently linked, e.g., ligated, in the
presence of a splint oligonucleotide. In an embodiment, the splint oligonucleotide is hybridized to a
sequence comprising the junction of the HC strand and the LC strand, or a sequence complementary
thereof, and forms a duplexed region at the site of on. In an ment, the splint
oligonucleotide comprises a modification (e.g., an NH; group) that inhibits DNA synthesis, e.g., by a
DNA polymerase. In an embodiment, the modification is at the 3’ end of the splint oligonucleotide.
In an embodiment, a strand complimentary to the ntly linked, e.g., ligated, HC and LC
strands is produced by amplification.
In an embodiment, the method, e.g., the step of covalent linkage, does not include a step of
overlap extension polymerase chain reaction (OE-PCR), also known as splicing by p extension
or splicing by overhang extension (SOE) PCR.
In an embodiment, the method further comprises, prior to ing the isolated production
reaction site, e.g., a production micro-chamber, acquiring an mRNA loaded capture ate.
In an embodiment, acquiring the mRNA loaded e substrate comprising: a) acquiring an
isolated cell reaction site, e.g., a cell isolation micro-chamber, comprising: i) a cell; and ii) a capture
substrate capable of binding a first mRNA encoding an HCVR from the cell and a second mRNA
encoding an LCVR from the cell; and b) maintaining the isolated cell reaction site, e.g., the cell
isolation micro-chamber, under conditions that allow lysis of the cell and binding of the capture
substrate with the first mRNA and the second mRNA to form the mRNA loaded e substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a nucleic
acid encoding an HCVR or an LCVR from a cell other than the cell (e.g., a different cell).
In an ment, the isolated cell reaction site, e.g., cell isolation micro-chamber, comprises
a lysing reagent, e.g., a detergent. In an embodiment, the cell is lysed by heat or an enzyme. In an
ment, the capture substrate comprises a moiety (e.g., an oligonucleotide) which binds mRNA,
6.3., an dT).
In an embodiment, the method further comprises releasing the mRNA loaded capture
substrate from the ed cell reaction site, e.g., the cell isolation micro-chamber. In an
embodiment, the releasing step is performed in the presence of a poly(dA) or T)
oligonucleotide, e.g., to reduce cross-binding of non-captured mRNA.
In an embodiment, the mRNA loaded capture substrate is transferred from the isolated cell
reaction site, e.g., the cell isolation micro-chamber, to the isolated production reaction site, e.g., the
production micro-chamber.
In an embodiment, the method further comprises releasing the c acid sequence from the
isolated tion reaction site, e.g., the tion micro-chamber. In an embodiment, the method
further comprises amplifying the nucleic acid sequence. In an ment, amplification of the
nucleic acid sequence occurs outside the isolated production reaction site, e.g., the production micro-
chamber, e.g., after the nucleic acid is ed from the isolated production reaction site, e.g., the
production chamber. In an embodiment, amplification of the nucleic acid sequence occurs at
the ed tion reaction site, e. g., the production micro-chamber.
In an embodiment, the method further comprises sequencing all or a portion of the nucleic
acid sequence.
In an embodiment, the method further comprises inserting all or a portion of nucleic acid
sequence into a vector. In an embodiment, the vector supplies an additional HC element or LC
element not included in the nucleic acid sequence. In an embodiment, the vector supplies an HC
CDRl, an HC CDRZ, or both. In an embodiment, the method further comprises sing the
vector.
In an embodiment, the method r comprises expressing the nucleic acid sequence to
produce a polypeptide comprising a segment that encodes an HC element of the HCVR, e.g., an
HCVRS, and a segment that encodes an LC element of the LCVR, e.g., an LCVRS. In an
embodiment, the LC element is inal to the HC element in the polypeptide. In an embodiment,
the HC element is C-terminal to the LC element in the polypeptide.
In an embodiment, the method further comprises contacting the polypeptide with an antigen.
In an embodiment, the method further ses determining if the polypeptide binds the antigen, in
vitro, ex vivo, or in viva, e.g., by a method or assay described herein.
In an aspect, the sure features a method of making a nucleic acid sequence comprising a
sequence that encodes a heavy chain element (HC element) of an antibody heavy chain variable
region (HCVR) and a light chain t (LC element) of an antibody light chain variable region
(LCVR), and wherein the HCVR and LCVR are matched, comprising:
a) acquiring an isolated cell reaction site (e.g., an isolated cell reaction site described herein),
6.3., a cell isolation micro-chamber, comprising: i) a cell (e.g., a cell described herein); and ii) a
capture substrate (e. g., a e substrate described herein) capable of binding a first mRNA
encoding an HCVR from the cell and a second mRNA encoding an LCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the e substrate with the first mRNA and the
second mRNA to form an mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell ion micro-chamber, does not include a
nucleic acid encoding an HCVR or an LCVR from a cell other than the cell (e.g., a different cell);
c) contacting the mRNA loaded capture substrate with a reaction mixture, e.g., a reaction
mixture comprising reverse transcriptase, that uses the loaded mRNA as a template to make cDNA
(this can occur, e.g., in the isolated cell reaction site, in an isolated production reaction site, or in
neither, e.g., not in an isolated reaction site);
d) acquiring an isolated production reaction site (e.g., an isolated production on site
described herein), e.g., a production chamber, comprising: i) a heavy chain (HC) strand,
wherein the HC strand is a strand of a heavy chain double-stranded cDNA (HC ds cDNA) comprising
a segment that encodes an HC element of the HCVR from the cell, e.g., a heavy chain variable region
sequence (HCVRS); and ii) a light chain (LC) strand, wherein the LC strand is a strand of a light
chain double-stranded cDNA (LC ds cDNA) comprising a segment that encodes an LC element of the
LCVR from the cell, e.g., a light chain variable region sequence ),
wherein the isolated production reaction site, e.g., a tion micro-chamber, does not
include a nucleic acid encoding an LCVR or an HCVR from a cell other than the cell (e.g., a different
cell); and
e) nt linking, e.g., ligation, of the HC strand to the LC strand.
In an embodiment, one or more (e.g., two, three, four, or all) of the steps a)-e) are med
in accordance with a method described . In an ment, each of the steps a)-e) is performed
in accordance with a method described herein.
In an aspect, the disclosure features a method of making a nucleic acid ce comprising a
sequence that encodes a heavy chain t (HC element) of an antibody heavy chain variable
region (HCVR) and a light chain element (LC element) of an antibody light chain variable region
(LCVR), and wherein the HCVR and LCVR are matched, comprising:
a) acquiring an isolated cell reaction site (e.g., an isolated cell reaction site described herein),
e.g., a cell isolation micro-chamber, comprising: i) a cell (e.g., a cell described herein); and ii) a
capture ate (e. g., a capture substrate described herein) capable of binding a first mRNA
encoding an HCVR from the cell and a second mRNA encoding an LCVR from the cell;
b) ining the isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a
c acid encoding an HCVR or an LCVR from a cell other than the cell (e.g., a different cell);
c) acquiring an isolated production reaction site (e.g., an isolated production reaction site
described herein), e.g., a production micro-chamber, comprises: contacting the mRNA loaded capture
substrate with a reaction mixture, e.g., a reaction mixture comprising reverse transcriptase, that uses
the loaded mRNA as a te, to produce: a first double-stranded cDNA (ds cDNA) comprising a
strand that is complementary to a first mRNA that s an HCVR from a cell; and a second ds
cDNA comprising a strand complementary to a second mRNA encoding an LCVR from the cell (the
cDNA loaded capture substrate);
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid ng an LCVR or an HCVR from a cell other than the cell (e. g., a different
cell).
d) ining the isolated production reaction site, e.g., the production micro-chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce: a ity of
HC ds cDNAs comprising a t that encodes an HC element of the HCVR from the cell, e. g., an
HCVRS; and a plurality of LC ds cDNAs comprising a t that encodes an LC element of the
LCVR from the cell, e.g., an LCVRS;
e) acquiring an isolated linkage reaction site (e.g., an isolated e reaction site described
herein), e.g., a linkage micro-chamber, comprising: covalent linking, e.g., ligation, of a strand of the
HC ds cDNA (HC strand) to a strand of the LC ds cDNA (LC strand), wherein the HC and LC strands
are both sense strands or antisense strands; and
t) amplifying the covalently , e.g., d, HC and LC s.
In an embodiment, one or more (e.g., two, three, four, five, or all) of the steps a)-f) are
performed in accordance with a method described herein. In an embodiment, each of the steps a)-f) is
performed in ance with a method described herein.
In an aspect, the disclosure features a method of making a library comprising a plurality of
unique members, the method comprising:
making the plurality of members, wherein each of the members ses a sequence that
encodes a heavy chain element (HC element) of a heavy chain variable region (HCVR) and a light
chain element (LC element) of a light chain variable region (LCVR), and wherein the HCVR and
LCVR are matched, made by a method described herein,
wherein each unique nucleic acid sequence of the plurality ses an HC t and an
LC element from a ent unique cell (e.g., a cell described herein),
thereby making a library comprising a plurality of unique members.
In an embodiment, the plurality of unique members ses at least 104, 105, 106, 107, 108,
or 109 unique members. In an embodiment, the plurality of unique members comprises 104 to 109, 104
to 10“, 104 to 107, 104 to 106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 108, 106 to
107, 104 to 105, 105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique members. In an embodiment, at
least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the members in the library are unique
members (which encode matched HC element and LC element sequences). In an embodiment, less
than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the members in the library are unique members
(which encode matched HC element and LC element sequences).
In an aspect, the disclosure features a y comprising a plurality of unique members,
wherein,
i) each unique member of the plurality comprises a segment that encodes an HC element, e.g.,
an HCVRS, and a segment that encodes an LC t, e. g., an LCVRS, wherein the HC element and
the LC element in each unique member is matched;
ii) each unique member of the ity comprises a segment that encodes an HC element,
6.3., an HCVRS, and a segment that encodes an LC t, e.g., an LCVRS, from a different unique
cell; and
iii) the library comprises one or more (e.g., two, three, four, or all) of the following
properties:
a) the library is made by a method described herein;
b) the plurality of unique members comprises at least 104, 105, 106, 107, 108, or 109
unique nucleic acid sequences;
c) the plurality of unique s comprises 104 to 109, 104 to 108, 104 to 107, 104 to
106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 108, 106 to 107, 104 to 105,
105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique members;
d) at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the members in
the y are unique members (which encode matched HC element and LC element
sequences); or
e) less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the members in the library
are unique s (which encode matched HC element and LC element sequences).
In an ment, each unique member of the plurality is configured such that, when
expressed, the HC element, e.g., the HCVRS, and the LC element, e.g., the LCVRS, form a functional
antigen binding molecule, e.g., an scFv, an Fab, or an scFab.
In an embodiment, the library is a display library. In an embodiment, each of the members of
the plurality further encodes a polypeptide that results in display of the member on the surface of a
display entity. In an embodiment, the library is a phage display library. In an embodiment, the
y is a yeast display y. In an embodiment, the library is a mammalian display library.
In an aspect, the disclosure features a method of making a binding polypeptide (e.g., a
polypeptide comprising an HC element and an LC element), the method comprising: a) acquiring a
y described herein, e.g., by a method described herein; and b) expressing a polypeptide encoded
by a unique nucleic acid of the library.
In an embodiment, the method further ses contacting the polypeptide with an antigen.
In an embodiment, the method further comprises retrieving (e.g., isolating or purifying) the nucleic
acid that s a polypeptide that binds the antigen.
In an aspect, the disclosure features an isolated production reaction site, e.g., a production
micro-chamber, which is an isolated production reaction site described herein (e.g., sing a
nucleic acid encoding an HCVR and a nucleic acid encoding a LCVR, wherein the HCVR and the
LCVR are matched).
In an embodiment, the isolated production on site, e.g., a production chamber,
does not include a nucleic acid encoding an HCVR or an LCVR from a different cell.
In an embodiment, the isolated production reaction site, e.g., a production micro-chamber,
comprises one, two, or all of: (i) one or more s specific to V gene sequences of the HC and LC;
(ii) one or more primers specific to overhangs uced onto the HC and LC cDNAs; or (iii) one or
more primers comprising a first member, a second member, and a third member comprising a
nucleotide modification (e.g., a ) located between the first and second members, wherein the
first member is capable of annealing with the second member of the same primer or a different
, e.g., forming a structure comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, ll, 12, l3, 14, 15,
16, 17, 18, 19, 20, more basepairs.
In an embodiment, the ed production reaction site, e. g., a production micro-chamber,
does not comprise a reagent that can covalently link nucleic acids, e.g., a ligase, e.g., a thermostable
ligase. In another embodiment, the isolated tion reaction site, e.g., a production micro-
chamber, comprises a reagent that can covalently link c acids, e.g., a ligase, e.g., a thermostable
1i gase.
In an aspect, the sure features a self-annealing oligonucleotide comprising a first
member, a second member, and third member comprising a nucleotide modification (e.g., a spacer)
located n the first and second members, n the first member is capable of annealing with
the second member of the same oligonucleotide (e.g., for a method of making a nucleic acid sequence
comprising a sequence that encodes an HC element of an HCVR and a LC element of an LCVR,
wherein the HCVR and LCVR are matched).
In an ment, the first and second members are e of forming a hairpin structure
comprising a duplex region of 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more
basepairs. In an embodiment, the first member is 5-40 nucleotides, 6.3., 5-10, 5-20, 5-30, 30-40, 20—
40, 10-30, 10-30, or 15-25 nucleotides, in length. In an embodiment, the second member is 5-40
nucleotides, e.g., 5-10, 5-20, 5-30, 30-40, 20-40, 10-30, 10-30, or 15-25 nucleotides, in length.
In an embodiment, the spacer is a spacer described herein, e.g., a flexible spacer or a PEG
In an ment, the first member comprises a sequence that is complementary to the
sequence of an oligonucleotide ed to a capture substrate.
In an embodiment, the second member comprises (e.g., from 5’ to 3’) one, two, or all of: (i) a
sequence that is complementary to at least a portion of the first member; (ii) a universal priming
sequence (e.g., for PCR amplification or next-generation sequencing); and (iii) a sequence
complementary to a target sequence, e.g., an HCVRS and/or an LCVRS. In an embodiment, the
universal priming sequence is identical, or substantially identical, to the sequence that is
complementary to at least a portion of the first member. In another embodiment, the universal
priming sequence is different from the sequence that is mentary to at least a portion of the first
member. In an embodiment, the second member ses a sequence for homologous
recombination (e.g., in a yeast or mammalian cell).
In an aspect, the disclosure features an isolated linkage reaction site, e. g., a e micro-
chamber, which is an isolated linkage reaction site described herein (e.g., sing a nucleic acid
encoding an HCVR and a nucleic acid encoding a LCVR, wherein the HCVR and the LCVR are
matched).
In an embodiment, the isolated linkage reaction site, e.g., a linkage micro-chamber, does not
include a nucleic acid encoding an HCVR or an LCVR from a different cell.
In an embodiment, the isolated linkage reaction site, e.g., a linkage micro-chamber, comprises
a splint oligonucleotide (e.g., a splint oligonucleotide described herein) that is capable of hybridizing
to a sequence sing the junction of the HC strand and the LC strand, or a sequence
complementary thereof, to form a ed region at the site of on.
In an ment, the isolated linkage reaction site, e. g., a linkage micro-chamber, comprises
a reagent that can covalently link nucleic acids, e.g., a ligase, e.g., a thermostable ligase,
In an aspect, the disclosure features a method of making a nucleic acid sequence comprising a
sequence that encodes an a chain t (AC element) of a TCR 0L chain le region (ACVR)
and a [3 chain element (BC element) of a TCR [3 chain variable region (BCVR), and wherein the
ACVR and the BCVR are matched, the method comprising:
a) acquiring an ed production reaction site, e.g., a production chamber,
comprising:
i) an a chain (AC) strand, wherein the AC strand is a strand of an or chain double-stranded
cDNA (AC ds cDNA) comprising a t that encodes an AC element of the ACVR from a cell,
e.g., an or chain variable region sequence (ACVRS); and
ii) a [5 chain (BC) strand, n the BC strand is a strand of a [3 chain ds cDNA (BC ds
cDNA) comprising a t that encodes a BC element of the BCVR from the cell, e.g., a [3 chain
variable region sequence (BCVRS), and
b) covalent linking, e. g., ligation, of the first strand to the second strand,
n the isolated production reaction site, e.g., a production chamber, does not
include a nucleic acid encoding an ACVR or a BCVR from a cell other than the cell (e.g., a different
cell, e.g., a different T cell),
thereby making a nucleic acid sequence sing a sequence that encodes an AC element
of an ACVR and a BC element of a BCVR, wherein the ACVR and the BCVR are matched.
In an embodiment, the AC element comprises, or consists of, an ACVRS, or a functional
fragment thereof (e.g., an antigen binding fragment thereof). In an embodiment, the BC element
comprises, or consists of, a BCVRS, or a functional fragment thereof (e.g., an antigen binding
fragment thereof).
In an embodiment, the AC ds cDNA comprises a segment that s an ACVRS. In an
embodiment, the BC ds cDNA comprises a segment that encodes a BCVRS. In an embodiment, the
AC ds cDNA comprises a segment that encodes an ACVRS, and the BC ds cDNA comprises a
segment that encodes a BCVRS.
In an embodiment, the cell is an immune cell, e.g., a T cell, 6.3., a human T cell. In an
embodiment, the cell is a mammalian cell or an avian cell.
In an embodiment, the nucleic acid sequence is configured such that, when expressed, the AC
t and the BC element (e.g., the ACVRS and the BCVRS) form a functional antigen binding
molecule, e.g., a single chain or a complex of a TCR or chain and a [3 chain. In an embodiment, the
antigen binding molecule, e.g., a TCR a chain andior a [3 chain, is functional in vitro, ex vivo, or in
vivo, e.g., as determined by a method or assay described herein.
In an embodiment, acquiring an isolated production reaction site, e.g., a production micro-
chamber, comprises:
a) acquiring a capture substrate bound to: (i) a first double-stranded cDNA (ds cDNA)
comprising a strand that is complementary to a first mRNA that encodes an ACVR from a cell; and
(ii) a second ds cDNA comprising a strand complementary to a second mRNA encoding a BCVR
from the cell (the cDNA loaded capture substrate), and
b) maintaining the isolated production reaction site, e.g., the tion chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce: a plurality of
AC ds cDNAs comprising a segment that encodes an AC element of the ACVR from the cell, e. g., an
ACVRS; and a plurality of BC ds cDNAs comprising a segment that encodes a BC element of the
BCVR from the cell, e.g., a BCVRS.
In an embodiment, the AC ds cDNA is cal, or substantially cal, to the first ds
cDNA. For example, the sense strand of the AC ds cDNA is at least 80%, 85 %, 90%, 95%, 98%,
99%, or 100% identical to, or differs by no more than 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50
nucleotides from, the sense strand of the first ds cDNA, and/or the antisense strand of the AC ds
cDNA is at least 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to, or s by no more than 1,
2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50 nucleotides from, the antisense strand of the first ds cDNA.
In an embodiment, the BC ds cDNA is identical, or ntially identical, to the second ds
cDNA. For example, the sense strand of the BC ds cDNA is at least 80%, 85%, 90%, 95%, 98%,
99%, or 100% identical to, or differs by no more than 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50
nucleotides from, the sense strand of the second ds cDNA, and/or the antisense strand of the BC ds
cDNA is at least 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to, or differs by no more than 1,
2, 5, 10, 15, 20, 25, 30, 35, 40, 45 , or 50 nucleotides from, the antisense strand of the second ds
cDNA.
In an embodiment, the AC strand is a sense strand. In an embodiment, the BC strand is a
sense strand. In an embodiment, the AC strand is an antisense strand. In an embodiment, the BC
strand is an antisense strand. In an ment, both the AC strand and the BC strand are sense
strands. In an ment, both the AC strand and the BC strand are nse strands.
In an embodiment, the capture ate comprises a bead, e.g., a magnetic bead. In an
embodiment, the capture substrate comprises a moiety (e.g., an oligonucleotide) which binds to
cDNA, e.g., (i) a moiety which binds to the AC strand; (ii) a moiety which binds to the BC strand; or
(iii) both (i) and (ii). In an embodiment, the moiety which binds to the AC strand is different from the
moiety which binds to the BC strand, e.g., to facilitate creating conditions favorable to ing
similar levels of each DNA molecule type. In an embodiment, the moiety which binds to the AC
strand is identical to the moiety which binds to the BC strand.
In an embodiment, the first mRNA and the second mRNA are disposed on an mRNA loaded
capture substrate.
In an embodiment, the isolated production reaction site, e.g., the production micro-chamber,
comprises: a reagent mixture suitable for producing, from the first and second mRNAs (e.g., after the
first and second mRNAs are released from the loaded mRNA capture substrate into a solution), a first
cDNA comprising a t that encodes an AC element of the ACVR of the cell, e.g., an ACVRS,
and a second cDNA comprising a segment that encodes a BC element of the BCVR of the cell, e.g., a
BCVRS.
In an ment, the isolated production reaction site, e.g., production micro-chamber,
comprises primers that mediate the production of the first ds cDNA. In an embodiment, the isolated
production reaction site, e.g., production micro-chamber, comprises primers that mediate the
production of the second ds cDNA.
In an embodiment, a cDNA strand that is complementary to a first mRNA that encodes an
ACVR from a cell is made by reverse transcription of the first mRNA. In an ment, a cDNA
strand that is complementary to a second mRNA that encodes a BCVR from a cell is made by reverse
transcription of the second mRNA.
In an embodiment, the reverse transcription takes place in the isolated production reaction
site, e.g., a production-micro chamber. In an embodiment, the e transcription takes place in an
isolated cell reaction site, e.g., a cell isolation chamber. In an embodiment, the reverse
transcription takes place outside the isolated production reaction site, e.g., a production micro-
chamber, or outside an isolated cell reaction site, e.g., a cell isolation micro-chamber. In an
embodiment, the reverse transcription takes place e the isolated production reaction site, e. g., a
production-micro chamber, and outside an isolated cell reaction site, e.g., a cell isolation micro-
chamber. In an embodiment, the reverse transcription takes place outside an isolated on site,
6.3., outside a micro-chamber.
In an embodiment, the amplification ses 20 or fewer cycles, e. g., 15 or fewer, 14 or
fewer, 13 or fewer, 12 or fewer, 11 or fewer, 10 or fewer, 9 or fewer, 8 or fewer, 7 or fewer, 6 or
fewer, or 5 or fewer cycles.
In an embodiment, the reverse transcription and/or amplification uses one or more primers,
e.g., comprising a sequence specific for an ACVRS and/or a BCVRS.
In an embodiment, the reverse transcription and/or amplification comprises using two or more
primers that mediate the production of the AC ds cDNA, wherein at least one primer ses a
nucleotide modification, and wherein at least one primer does not comprise a nucleotide modification.
In an embodiment, the amplification comprises using two or more primers that mediate the production
of the BC ds cDNA, n at least one primer comprises a nucleotide modification, and wherein at
least one primer does not comprise a nucleotide modification.
In an embodiment, at least one primer comprises a nucleotide modification, e.g., which
reduces, e.g., inhibits, DNA synthesis, e.g., by a DNA polymerase. In an embodiment, at least one
primer does not comprise a tide modification, e.g., which s, e.g., inhibits, DNA
sis, e.g., by a DNA polymerase.
In an embodiment, the nucleotide modification inhibits a DNA polymerase from ing
the DNA. Without wishing to be bound by theory, it is believed that in an embodiment, any chemical
entity that reduces (e.3., blocks) DNA polymerase extension can be used in accordance with the
methods described herein.
In an embodiment, the nucleotide modification is an insertion of a spacer to the primer, e. g.,
between two adjacent nucleotides in the primer. In an embodiment, the spacer is a e spacer. In
an embodiment, the spacer is a carbon spacer (e.g., -(CH2)n-, n n=3, 4, 5, 6, 7, 8, 9, 10, or
more), two or more (e.g., three, four, five, six, seven, eight, nine, ten, or more) abasic nucleotides, or a
polyethylene glycol (PEG) spacer. In an embodiment, the spacer is a PEG spacer. In an ment,
the nucleotide modification is 2’-O-methyl, 2’-OH, 2’-NH2, or uracil, e.g., to a ribose.
In an embodiment, the tide modification is located internally or at the 3’ end of the
primer. In an embodiment, at least one primer comprises (i) a first member; (ii) a second ;
and optionally (iii) a third member, e.g., sing a nucleotide modification described herein, e.g.,
located between (i) and (ii).
In an embodiment, the first member is capable of annealing with the second member. In an
embodiment, the first member is capable of ing with the second member in the same primer,
e.g., through intra-molecular ization, e.g., to form a hairpin structure comprising a duplex
region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more basepairs. In r
ment, the first member is capable of annealing hybridizing with the second member in a
different primer, e. g., through inter-molecular hybridization, e. g., to form a double-stranded structure
comprising a duplex region of 4, 5,6, 7, 8, 9, 10, ll, 12, l3, 14, 15, l6, l7, 18, 19, 20, more
basepairs. Without wishing to be bound by theory, it is believed that in an embodiment, there are at
least two secondary structures that the modified primers can form and facilitate reduction (e.g.,
prevention) of ition to substrate (e.g., bead) capture. For example, the secondary structure can
be a hairpin-like structure formed by molecular hybridization (within the same primer), or the
secondary structure can be a duplex structure formed by inter-molecular hybridization (between two
different primers).
In an embodiment, the first member comprises a sequence that is complementary to the
sequence of an oligonucleotide attached to the capture substrate. In an embodiment, the second
member comprises (6.3., from 5’ to 3’) one, two, or all of: (i) a sequence that is complementary to at
least a portion of the first member; (ii) a universal priming sequence (e.g., for PCR amplification or
next-generation sequencing); and (iii) a sequence complementary to a target sequence, e.g., an
ACVRS and/or a BCVRS. In an embodiment, the universal priming sequence is identical, or
substantially identical, to the sequence that is complementary to at least a portion of the first member.
In r embodiment, the universal priming sequence is different from the ce that is
complementary to at least a portion of the first . In an embodiment, the second member
comprises a sequence for homologous recombination (e.g., in a yeast or mammalian cell).
In an embodiment, at least one primer ses a sequence encoding at least a n of a
linker sequence, or a complementary sequence thereof. In an embodiment, the primer that comprises
a sequence ng at least a portion of a linker sequence, or a complementary sequence thereof, is
phosphorylated, e.g., 5’ phosphorylated. Without wishing to be bound by theory, it is believed that in
an embodiment, any sequence with the general ties of flexibility (e.g., facilitated by glycine)
and hydrophilicity can work effectively in accordance with the methods described herein. Exemplary
linkers can generally have overrepresentation of one or more of Gly, Ser, Thr, or Ala and
underrepresentation of hydrophobic residues, e.g., one or more of Trp, Tyr, Phe, Cys, Met, Leu, or Ile.
The length of the primer may vary, e.g., 3-50 amino acid residues (e.g., 5-45, 10-40, 15-35, 20-30, 10-
20, 10-30, 20-40, or 30-40 amino acid residues). In an embodiment, the linker sequence comprises, or
consists of, ((Gly)m-Ser))n, where m=3, 4, 5, or more and n=1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more. In an
ment, the linker ce comprises, or consists of, (Gly-Gly-Gly-Gly-Ser)n, where n=1, 2, 3,
4, 5, 6, 7, 8, 9, 10, or more.
In an embodiment, the primer is a primer described herein, e. g., in Examples.
In an embodiment, the reverse transcription, the amplification, or both, occurs in a solution in
the isolated production reaction site, e.g., production micro-chamber. In an embodiment, the reverse
transcription, the amplification, or both, does not occur on the substrate (e.g., bead). For example, the
reverse ription, the amplification, or both, can occur on in a solution within a droplet.
In an embodiment, the AC ds cDNA comprises a 5’ overhang, e. g., a 5’ overhang that is
capable of hybridizing to an oligonucleotide attached to a capture substrate. In an embodiment, the
AC ds cDNA ses a blunt end, e.g., a blunt end comprising a 5’ phosphate. In an embodiment,
the BC ds cDNA comprises a 5’ ng, e.g., a 5’ ng that is capable of hybridizing to an
oligonucleotide attached to a e substrate. In an embodiment, the BC ds cDNA comprises a
blunt end, e.g., a blunt end comprising a 5’ phosphate. In an embodiment, the AC ds cDNA and the
BC ds cDNA comprise sticky ends, e.g., both have 5’ overhangs.
In an embodiment, the AC strand and the BC strand are ntly linked, e.g., ligated, to
produce a single stranded nucleic acid sequence, wherein the AC and BC strands are both sense
strands or both antisense strands. In an embodiment, a denatured AC strand of the AC ds cDNA to a
denatured BC strand of the BC ds cDNA are covalently linked, e.g., ligated, wherein the AC and BC
strands are both sense strands or both antisense strands. In an embodiment, the AC strand is present
in the AC ds cDNA and the BC strand is present in the BC ds cDNA, and wherein the AC ds cDNA
and the BC ds cDNA are covalently linked, e.g., ligated, e.g., to produce a double stranded nucleic
acid sequence.
In an embodiment, the covalent linking, e.g., on, occurs in the isolated production
reaction site. In an ment, the isolated production reaction site, e.g., a production micro-
chamber, or the ed e reaction site, e.g., a linkage micro-chamber, comprises a reagent that
is capable of covalently linking, e.g., ligating, the AC and BC strands or the AC and BC ds cDNAs.
In an embodiment, the isolated tion reaction site, e.g., a production micro-chamber comprises
an enzyme that covalently couples the AC and BC strands or the AC and BC ds cDNAs. In an
embodiment, the enzyme is a ligase, e.g., a thermal stable ligase. In an embodiment, the covalent
linking comprises ligase thermocycling.
In an ment, the covalent linking, e. g., on, occurs in a site different from the
isolated production reaction site, e.g., occurs in an isolated linkage reaction site, e.g., a linkage micro-
chamber. In an embodiment, the AC strand and the BC strand are transferred from the isolated
production site to the isolated linkage reaction site, e.g., a linkage micro-chamber, and the covalent
linking occurs in the isolated linkage reaction site, e.g., a linkage micro-chamber. In an embodiment,
the isolated linkage on site, e.g., a linkage micro-chamber, comprises a t that is e of
covalently linking, e.g., ligating, the AC and BC strands or the AC and BC ds cDNAs. In an
embodiment, the isolated linkage reaction site, e.g., a linkage micro-chamber, ses an enzyme
that covalently couples the AC and BC s or the AC and BC ds cDNAs. In an embodiment, the
enzyme is a ligase, e.g., a thermal stable ligase. In an embodiment, the covalent linking ses
ligase thermocycling.
In an embodiment, the covalent linking, e.g., ligation, comprises: (a) heating the isolated
linkage reaction site, e. g., the linkage micro-chamber, under conditions (e.g., at 950C) that allow
denaturation of the AC strand and the BC ; (b) cooling the isolated linkage reaction site, e. g.,
the linkage micro-chamber, under conditions (e.g., at 50-65°C) that allow hybridization of the splint
oligonucleotide to the AC strand and the BC strand; (c) maintaining the isolated linkage reaction site,
6.3., the linkage micro-chamber, under conditions (e.g., at 45-650C) that allow on of the AC
strand and the BC strand (e.g., formation of phosphodiester bond between the AC strand and the BC
strand); and (d) repeating steps (a), (b), and (c) sequentially for 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50,
or more cycles.
In an embodiment, the AC strand and the BC strand are covalently linked, e.g., ligated, in the
presence of a splint ucleotide. In an ment, the splint oligonucleotide is hybridized to a
sequence comprising the junction of the AC strand and the BC strand, or a sequence complementary
thereof, and forms a duplexed region at the site of ligation. In an embodiment, the splint
oligonucleotide comprises a modification (e.g., an NHZ group) that inhibits DNA synthesis, e.g., by a
DNA rase. In an ment, the modification is at the 3’ end of the splint oligonucleotide.
In an embodiment, a strand complimentary to the ntly linked, e.g., ligated, AC and BC
strands is produced by amplification.
In an embodiment, the method, e.g., the step of covalent linkage, does not include a step of
overlap extension polymerase chain on (OE-PCR), also known as splicing by overlap extension
or splicing by overhang extension (SOE) PCR.
In an embodiment, the method further comprises, prior to acquiring the isolated production
reaction site, e.g., a production micro-chamber, acquiring an mRNA loaded capture substrate.
In an ment, ing the mRNA loaded capture substrate comprising: a) ing an
isolated cell reaction site, e.g., a cell isolation micro-chamber, comprising: i) a cell; and ii) a capture
substrate capable of binding a first mRNA ng an ACVR from the cell and a second mRNA
encoding a BCVR from the cell; and b) maintaining the isolated cell reaction site, e.g., the cell
isolation micro-chamber, under ions that allow lysis of the cell and binding of the capture
substrate with the first mRNA and the second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a nucleic
acid encoding an ACVR or a BCVR from a cell other than the cell (e.g., a different cell).
In an embodiment, the isolated cell reaction site, e.g., cell isolation micro-chamber, comprises
a lysing reagent, e.g., a detergent. In an embodiment, the cell is lysed by heat or an enzyme. In an
embodiment, the capture substrate comprises a moiety (e.g., an oligonucleotide) which binds mRNA,
e.g., an dT).
In an embodiment, the method further comprises releasing the mRNA loaded capture
substrate from the isolated cell reaction site, e.g., the cell isolation micro-chamber. In an
ment, the releasing step is med in the presence of a A) or poly(dT)
oligonucleotide, e.g., to reduce cross-binding of non-captured mRNA.
In an embodiment, the mRNA loaded capture substrate is transferred from the isolated cell
reaction site, e.g., the cell isolation micro-chamber, to the isolated production reaction site, e.g., the
production micro-chamber.
In an ment, the method further comprises releasing the nucleic acid sequence from the
isolated production reaction site, e.g., the tion micro-chamber. In an embodiment, the method
further comprises amplifying the nucleic acid sequence. In an embodiment, amplification of the
nucleic acid sequence occurs outside the isolated production reaction site, e.g., the production micro-
chamber, e. g., after the nucleic acid is released from the ed tion reaction site, e.g., the
production micro-chamber. In an embodiment, amplification of the nucleic acid ce occurs at
the ed production reaction site, e.g., the production micro-chamber.
In an embodiment, the method further comprises sequencing all or a portion of the c
acid sequence.
In an embodiment, the method further comprises inserting all or a portion of nucleic acid
sequence into a vector. In an embodiment, the vector supplies an additional AC element or BC
element not included in the nucleic acid sequence. In an embodiment, the method further comprises
expressing the vector.
In an embodiment, the method further comprises expressing the nucleic acid sequence to
e a polypeptide comprising a segment that s an AC element of the ACVR, e. g., an
ACVRS, and a segment that encodes a BC element of the BCVR, e.g., a BCVRS. In an embodiment,
the BC element is N—terminal to the AC element in the polypeptide. In an embodiment, the AC
element is C-terminal to the BC element in the polypeptide.
In an ment, the method further comprises contacting the polypeptide with an antigen.
In an embodiment, the method r comprises determining if the polypeptide binds the antigen, in
vitro, ex vivo, or in viva, e.g., by a method or assay bed herein.
In an embodiment, the disclosure features a method of making a nucleic acid sequence
comprising a sequence that encodes a TCR 0L chain element (AC element) of TCR 0L chain variable
region (ACVR) and a TCR [3 chain element (BC element) of a TCR [3 chain variable region (BCVR),
and wherein the ACVR and BCVR are matched, comprising:
a) acquiring an isolated cell on site (e.g., an isolated cell reaction site described herein),
e.g., a cell isolation micro-chamber, comprising: i) a cell (e.g., a cell described herein); and ii) a
capture substrate (e.g., a capture substrate described herein) capable of binding a first mRNA
encoding an ACVR from the cell and a second mRNA encoding a BCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form an mRNA loaded e substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not e a
nucleic acid encoding an ACVR or a BCVR from a cell other than the cell (e.g., a different cell);
c) contacting the mRNA loaded capture substrate with a reaction mixture, e.g., a reaction
mixture comprising e transcriptase, that uses the loaded mRNA as a template to make cDNA
(this can occur, e.g., in the isolated cell on site, in an isolated tion reaction site, or in
neither, e.g., not in an isolated reaction site);
d) acquiring an isolated production reaction site (e. g., an isolated production on site
described herein), e.g., a production micro-chamber, comprising: i) a TCR 0L chain (AC) strand,
wherein the AC strand is a strand of a TCR a chain double-stranded cDNA (AC ds cDNA)
comprising a segment that encodes an AC element of the ACVR from the cell, e.g., a TCR 0L chain
variable region sequence (ACVRS); and ii) a TCR [5 chain (BC) , wherein the BC strand is a
strand of a TCR [3 chain double-stranded cDNA (BC ds cDNA) comprising a segment that encodes a
BC element of the BCVR from the cell, e. g., a TCR [3 chain variable region sequence (BCVRS),
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding an ACVR or a BCVR from a cell other than the cell (e.g., a different
cell); and
e) covalent linking, e.g., ligation, of the AC strand to the BC .
In an embodiment, one or more (e.g., two, three, four, or all) of the steps a)-e) are performed
in accordance with a method bed herein. In an embodiment, each of the steps a)-e) is performed
in accordance with a method bed herein.
In an aspect, the disclosure features a method of making a nucleic acid sequence comprising a
sequence that encodes a TCR 0c chain element (AC t) of a TCR a chain variable region
(ACVR) and a TCR B chain element (BC element) of a TCR [3 chain variable region (BCVR), and
wherein the ACVR and BCVR are matched, comprising:
a) acquiring an isolated cell reaction site (e.g., an isolated cell on site described herein),
e.g., a cell ion chamber, comprising: i) a cell (e.g., a cell described herein); and ii) a
capture substrate (e. g., a capture substrate described herein) capable of binding a first mRNA
encoding an ACVR from the cell and a second mRNA encoding a BCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell ion micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a
nucleic acid encoding an ACVR or a BCVR from a cell other than the cell (e.g., a different cell);
c) ing an isolated production reaction site (e.g., an isolated production reaction site
described ), e.g., a production micro-chamber, ses: contacting the mRNA loaded capture
substrate with a reaction mixture, e.g., a reaction mixture comprising e transcriptase, that uses
the loaded mRNA as a te, to produce: a first double-stranded cDNA (ds cDNA) comprising a
strand that is complementary to a first mRNA that encodes an ACVR from a cell; and a second ds
cDNA comprising a strand complementary to a second mRNA encoding a BCVR from the cell (the
cDNA loaded e substrate);
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding an ACVR or a BCVR from a cell other than the cell (e. g., a different
cell).
d) maintaining the isolated production reaction site, e.g., the production micro-chamber,
under ions that allow amplification of the first and second ds cDNAs, to produce: a plurality of
AC ds cDNAs comprising a segment that s an AC element of the ACVR from the cell, e.g., an
ACVRS; and a plurality of BC ds cDNAs comprising a segment that encodes a BC element of the
BCVR from the cell, e.g., a BCVRS;
e) acquiring an isolated linkage reaction site (e.g., an ed linkage reaction site described
herein), e.g., a linkage micro-chamber, comprising: covalent linking, e. g., ligation, of a strand of the
AC ds cDNA (AC strand) to a strand of the BC ds cDNA (BC strand), n the AC and BC
strands are both sense strands or antisense strands; and
f) amplifying the covalently linked, e.g., ligated, AC and BC strands.
In an embodiment, one or more (e. g., two, three, four, five, or all) of the steps a)-f) are
performed in accordance with a method described herein. In an embodiment, each of the steps a)-f) is
performed in accordance with a method described herein.
In an aspect, the disclosure features a method of making a library comprising a plurality of
unique members, the method comprising:
making the plurality of members, wherein each of the members comprises a sequence that
encodes a TCR a chain element (AC element) of a TCR a chain variable region (ACVR) and a TCR [3
chain element (BC t) of a TCR [3 chain le region (BCVR), and wherein the ACVR and
BCVR are matched, made by a method described ,
wherein each unique nucleic acid sequence of the plurality comprises an AC t and a
BC element from a different unique cell (e. g., a cell described herein),
thereby making a library comprising a plurality of unique members.
In an embodiment, the plurality of unique members ses at least 104, 105, 106, 107, 108,
or 109 unique s. In an embodiment, the plurality of unique members ses 104 to 109, 104
to 108, 104 to 107, 104 to 106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 10", 106 to
107, 104 to 105, 105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique s. In an embodiment, at
least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the members in the library are unique
members (which encode d AC element and BC element sequences). In an embodiment, less
than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the members in the library are unique members
(which encode d AC element and BC element sequences).
In an aspect, the disclosure features a y comprising a plurality of unique members,
wherein,
i) each unique member of the plurality comprises a segment that encodes an AC element, e.g.,
an ACVRS, and a segment that encodes a BC element, e.g., a BCVRS, wherein the AC element and
the BC element in each unique member is matched;
ii) each unique member of the plurality comprises a segment that encodes an AC element,
e.g., an ACVRS, and a segment that encodes a BC t, e.g., a BCVRS, from a different unique
cell; and
iii) the library comprises one or more (e.g., two, three, four, or all) of the following
properties:
a) the library is made by a method described herein;
b) the plurality of unique members comprises at least 104, 105, 106, 107, 108, or 109 unique
nucleic acid sequences;
c) the plurality of unique members comprises 104 to 109, 104 to 108, 104 to 107, 104 to 106, 104
to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 103, 106 to 107, 104 to 105, 105 to 106, 106 to
107, 107 to 108, or 108 to 109 unique members;
d) at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the members in the
library are unique members (which encode matched AC element and BC element sequences); or
e) less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the members in the library are
unique members (which encode matched AC element and BC element sequences).
In an embodiment, each unique member of the plurality is configured such that, when
expressed, the AC element, e.g., the ACVRS, and the BC element, e.g., the BCVRS, form a functional
antigen binding molecule, e.g., a single chain or a x of a TCR a chain and a [3 chain.
In an embodiment, the library is a display library. In an embodiment, each of the members of
the plurality further encodes a ptide that results in display of the member on the surface of a
display entity. In an embodiment, the library is a phage display library. In an embodiment, the
library is a yeast display library. In an embodiment, the library is a mammalian display library.
In an aspect, the disclosure es a method of making a binding ptide (e.g., a
polypeptide sing an AC element and a BC element), the method comprising: a) acquiring a
library described herein, e.g., by a method described herein; and b) expressing a polypeptide encoded
by a unique nucleic acid of the library.
In an embodiment, the method further comprises contacting the polypeptide with an antigen.
In an embodiment, the method r comprises retrieving (e.g., isolating or purifying) the c
acid that encodes a polypeptide that binds the antigen.
In an aspect, the disclosure features an isolated production reaction site, e.g., a production
micro-chamber, which is an isolated production reaction site described herein (e.g., comprising a
nucleic acid encoding an ACVR and a nucleic acid encoding a BCVR, wherein the ACVR and the
BCVR are matched).
In an embodiment, the isolated production reaction site, e.g., a production micro-chamber,
does not include a nucleic acid ng an ACVR or a BCVR from a ent cell.
In an embodiment, the isolated production on site, e.g., a production micro-chamber,
comprises one, two, or all of: (i) one or more primers specific to V gene sequences of the AC and BC;
(ii) one or more primers specific to overhangs introduced onto the AC and BC cDNAs; or (iii) one or
more primers comprising a first , a second member, and a third member comprising a
nucleotide modification (e.g., a spacer) located between the first and second s, n the
first member is capable of annealing with the second member of the same primer or a different
primer, e.g., forming a structure comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,
16, 17, 18, 19, 20, more basepairs.
In an embodiment, the isolated production reaction site, e.g., a production micro-chamber,
does not comprise a reagent that can ntly link nucleic acids, e.g., a ligase, e.g., a thermostable
1i gase. In another embodiment, the isolated production reaction site, e. g., a tion micro-
chamber, comprises a reagent that can covalently link nucleic acids, e.g., a ligase, e.g., a thermostable
ligase.
In an aspect, the disclosure features a self-annealing oligonucleotide comprising a first
member, a second , and third member comprising a nucleotide modification (e. g., a spacer)
located between the first and second members, wherein the first member is capable of annealing with
the second member of the same oligonucleotide (e.g., for a method of making a nucleic acid sequence
comprising a sequence that encodes an AC element of an ACVR and a BC element of a BCVR,
wherein the ACVR and BCVR are matched).
In an embodiment, the first and second members are e of forming a n structure
comprising a duplex region of 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more
basepairs. In an ment, the first member is 5-40 nucleotides, e.g., 5-10, 5-20, 5-30, 30-40, 20-
40, 10-30, 10-30, or 15-25 nucleotides, in length. In an embodiment, the second member is 5-40
nucleotides, e.g., 5-10, 5-20, 5-30, 30-40, 20-40, 10-30, 10-30, or 15-25 nucleotides, in length.
In an embodiment, the spacer is a spacer described , e.g., a flexible spacer or a PEG
spacer.
In an embodiment, the first member comprises a sequence that is complementary to the
sequence of an ucleotide attached to a capture substrate.
In an embodiment, the second member comprises (e.g., from 5’ to 3’) one, two, or all of: (i) a
sequence that is complementary to at least a portion of the first member; (ii) a universal priming
sequence (e.g., for PCR amplification or next-generation sequencing); and (iii) a sequence
complementary to a target sequence, e.g., an ACVRS and/or a BCVRS. In an embodiment, the
universal priming sequence is identical, or substantially identical, to the sequence that is
complementary to at least a portion of the first member. In r embodiment, the universal
g sequence is different from the sequence that is complementary to at least a portion of the first
member. In an embodiment, the second member comprises a sequence for homologous
recombination (e.g., in a yeast or mammalian cell).
In an aspect, the disclosure features an isolated linkage reaction site, e.g., a linkage micro-
chamber, which is an isolated linkage reaction site described herein (e.g., comprising a nucleic acid
encoding an ACVR and a nucleic acid encoding a BCVR, wherein the ACVR and the BCVR are
d).
In an embodiment, the isolated linkage on site, e.g., a linkage chamber, does not
include a nucleic acid encoding an ACVR or a BCVR from a different cell.
In an embodiment, the isolated linkage reaction site, 6. g., a linkage micro-chamber, comprises
a splint oligonucleotide (e.g., a splint oligonucleotide described herein) that is capable of izing
to a sequence comprising the junction of the AC strand and the BC strand, or a ce
complementary thereof, to form a duplexed region at the site of ligation.
WO 19402
In an embodiment, the isolated linkage on site, e.g., a linkage micro-chamber, ses
a reagent that can covalently link nucleic acids, e.g., a ligase, e.g., a thermostable ligase.
In an aspect, the disclosure features a method of making a nucleic acid sequence comprising a
sequence that encodes a 7 chain element (GC element) of a TCR V chain variable region (GCVR) and
a 5 chain element (DC element) of a TCR 5 chain variable region , and n the GCVR
and the DCVR are matched, the method comprising:
a) acquiring an isolated tion reaction site, e.g., a production micro-chamber,
comprising:
i) an y chain (GC) strand, n the GC strand is a strand of an 7 chain double-stranded
cDNA (GC ds cDNA) comprising a segment that encodes a GC element of the GCVR from a cell,
e.g., an 7 chain variable region sequence (GCVRS); and
ii) a 5 chain (DC) strand, wherein the DC strand is a strand of a 5 chain ds cDNA (DC ds
cDNA) comprising a segment that encodes a DC element of the DCVR from the cell, e. g., a 5 chain
variable region sequence (DCVRS), and
b) covalent linking, e.g., ligation, of the first strand to the second strand,
n the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding a GCVR or a DCVR from a cell other than the cell (e.g., a different
cell, e.g., a different T cell),
y making a nucleic acid sequence comprising a sequence that encodes a GC element of
a GCVR and a DC element of a DCVR, wherein the GCVR and the DCVR are matched.
In an embodiment, the GC element comprises, or consists of, a GCVRS, or a functional
fragment thereof (e.g., an antigen binding fragment thereof). In an embodiment, the DC element
ses, or consists of, a DCVRS, or a functional fragment thereof (e.g., an antigen binding
fragment thereof).
In an embodiment, the GC ds cDNA comprises a t that encodes a GCVRS. In an
ment, the DC ds cDNA comprises a segment that encodes a DCVRS. In an embodiment, the
GC ds cDNA comprises a segment that encodes a GCVRS, and the DC ds cDNA comprises a
segment that encodes a DCVRS.
In an embodiment, the cell is an immune cell, e.g., a T cell, 6.3., a human T cell. In an
embodiment, the cell is a mammalian cell or an avian cell.
In an embodiment, the nucleic acid sequence is configured such that, when expressed, the GC
element and the DC element (e.g., the GCVRS and the DCVRS) form a functional antigen binding
molecule, e.g., a single chain or a complex of a TCR 7 chain and a 5 chain. In an embodiment, the
antigen binding molecule, e.g., a TCR V chain and/or a 5 chain, is functional in vitro, ex vivo, or in
viva, e.g., as determined by a method or assay bed herein.
In an embodiment, ing an isolated production reaction site, e.g., a tion micro-
chamber, comprises:
a) acquiring a capture substrate bound to: (i) a first double-stranded cDNA (ds cDNA)
comprising a strand that is complementary to a first mRNA that encodes a GCVR from a cell; and (ii)
a second ds cDNA comprising a strand complementary to a second mRNA encoding a DCVR from
the cell (the cDNA loaded capture substrate), and
b) maintaining the isolated production reaction site, e.g., the production micro-chamber,
under conditions that allow cation of the first and second ds cDNAs, to produce: a plurality of
GC ds cDNAs comprising a segment that encodes a GC element of the GCVR from the cell, e.g., a
GCVRS; and a plurality of DC ds cDNAs comprising a segment that s a DC t of the
DCVR from the cell, e.g., a DCVRS.
In an embodiment, the GC ds cDNA is identical, or substantially identical, to the first ds
cDNA. For e, the sense strand of the GC ds cDNA is at least 80%, 85 %, 90%, 95%, 98%,
99%, or 100% identical to, or differs by no more than 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50
nucleotides from, the sense strand of the first ds cDNA, and/or the antisense strand of the GC ds
cDNA is at least 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to, or differs by no more than 1,
2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50 tides from, the antisense strand of the first ds cDNA.
In an embodiment, the DC ds cDNA is identical, or substantially identical, to the second ds
cDNA. For example, the sense strand of the DC ds cDNA is at least 80%, 85 %, 90%, 95%, 98%,
99%, or 100% identical to, or differs by no more than 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50
nucleotides from, the sense strand of the second ds cDNA, and/or the antisense strand of the DC ds
cDNA is at least 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to, or differs by no more than 1,
2, 5, 10, 15, 20, 25, 30, 35, 40, 45 , or 50 nucleotides from, the antisense strand of the second ds
cDNA.
In an embodiment, the GC strand is a sense strand. In an embodiment, the DC strand is a
sense strand. In an embodiment, the GC strand is an antisense strand. In an embodiment, the DC
strand is an antisense strand. In an ment, both the GC strand and the DC strand are sense
strands. In an embodiment, both the GC strand and the DC strand are antisense s.
In an embodiment, the capture substrate ses a bead, e.g., a magnetic bead. In an
embodiment, the capture substrate comprises a moiety (e.g., an oligonucleotide) which binds to
cDNA, e.g., (i) a moiety which binds to the GC strand; (ii) a moiety which binds to the DC strand; or
(iii) both (i) and (ii). In an embodiment, the moiety which binds to the GC strand is different from the
moiety which binds to the DC strand, e.g., to facilitate creating conditions favorable to capturing
similar levels of each DNA molecule type. In an embodiment, the moiety which binds to the GC
strand is identical to the moiety which binds to the DC strand.
In an embodiment, the first mRNA and the second mRNA are disposed on an mRNA loaded
capture substrate.
In an embodiment, the isolated production on site, e. g., the production micro-chamber,
comprises: a reagent e suitable for producing, from the first and second mRNAs (e.g., after the
first and second mRNAs are released from the mRNA loaded capture substrate into a solution), a first
cDNA comprising a segment that encodes a GC element of the GCVR of the cell, e.g., a GCVRS, and
a second cDNA sing a segment that encodes a DC element of the DCVR of the cell, e.g., a
DCVRS.
In an embodiment, the isolated production reaction site, e.g., production micro-chamber,
comprises primers that mediate the production of the first ds cDNA. In an embodiment, the isolated
production reaction site, e.g., production micro-chamber, comprises primers that mediate the
production of the second ds cDNA.
In an embodiment, a cDNA strand that is complementary to a first mRNA that encodes a
GCVR from a cell is made by reverse transcription of the first mRNA. In an embodiment, a cDNA
strand that is mentary to a second mRNA that encodes a DCVR from a cell is made by reverse
transcription of the second mRNA.
In an embodiment, the reverse transcription takes place in the isolated production reaction
site, e.g., a production-micro chamber. In an embodiment, the reverse transcription takes place in an
isolated cell on site, e.g., a cell isolation micro-chamber. In an embodiment, the reverse
transcription takes place outside the isolated production reaction site, e.g., a tion micro-
chamber, or outside an isolated cell on site, e.g., a cell isolation micro-chamber. In an
embodiment, the reverse transcription takes place outside the isolated production reaction site, e.g., a
production-micro chamber, and outside an isolated cell reaction site, e.g., a cell ion micro-
chamber. In an ment, the reverse transcription takes place outside an isolated reaction site,
e.g., e a micro-chamber.
In an ment, the amplification comprises 20 or fewer cycles, e.g., 15 or fewer, 14 or
fewer, 13 or fewer, 12 or fewer, 11 or fewer, 10 or fewer, 9 or fewer, 8 or fewer, 7 or fewer, 6 or
fewer, or 5 or fewer cycles.
In an ment, the e transcription and/or amplification uses one or more primers,
e.g., comprising a sequence specific for a GCVRS and/or a DCVRS.
In an embodiment, the e transcription and/or amplification comprises using two or more
primers that mediate the production of the GC ds cDNA, wherein at least one primer comprises a
nucleotide modification, and wherein at least one primer does not comprise a nucleotide modification.
In an embodiment, the amplification comprises using two or more primers that mediate the production
of the DC ds cDNA, wherein at least one primer comprises a nucleotide modification, and wherein at
least one primer does not comprise a nucleotide modification.
In an embodiment, at least one primer comprises a nucleotide modification, e. g., which
reduces, e.g., inhibits, DNA synthesis, e.g., by a DNA polymerase. In an embodiment, at least one
primer does not comprise a nucleotide modification, e.g., which reduces, e.g., inhibits, DNA
sis, e.g., by a DNA polymerase.
In an embodiment, the nucleotide modification inhibits a DNA polymerase from extending
the DNA. Without wishing to be bound by theory, it is ed that in an embodiment, any chemical
entity that reduces (e.g., blocks) DNA rase extension can be used in accordance with the
methods described herein.
In an embodiment, the nucleotide modification is an insertion of a spacer to the primer, e.g.,
between two adjacent nucleotides in the primer. In an embodiment, the spacer is a flexible spacer. In
an embodiment, the spacer is a carbon spacer (e.g., -(CH2)n-, wherein n=3, 4, 5, 6, 7, 8, 9, 10, or
more), two or more (e.g., three, four, five, six, seven, eight, nine, ten, or more) abasic nucleotides, or a
polyethylene glycol (PEG) spacer. In an embodiment, the spacer is a PEG spacer. In an embodiment,
the tide modification is ethyl, 2’-OH, 2’-NH2, or uracil, e.g., to a ribose.
In an embodiment, the tide modification is located internally or at the 3’ end of the
primer. In an ment, at least one primer comprises (i) a first member; (ii) a second member;
and optionally (iii) a third member, e.g., comprising a nucleotide modification described herein, e.g.,
located between (i) and (ii).
In an embodiment, the first member is capable of annealing with the second member. In an
embodiment, the first member is capable of annealing with the second member in the same primer,
e.g., through intra-molecular hybridization, e.g., to form a hairpin structure comprising a duplex
region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more basepairs. In another
embodiment, the first member is capable of annealing hybridizing with the second member in a
different , e.g., through inter-molecular hybridization, e.g., to form a double-stranded ure
comprising a duplex region of 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more
basepairs. t wishing to be bound by theory, it is believed that in an embodiment, there are at
least two secondary structures that the modified primers can form and facilitate reduction (e.g.,
prevention) of ition to substrate (e.g., bead) capture. For example, the secondary structure can
be a hairpin-like structure formed by intra-molecular hybridization (within the same primer), or the
secondary structure can be a duplex structure formed by inter-molecular hybridization (between two
different primers).
In an embodiment, the first member comprises a sequence that is complementary to the
sequence of an oligonucleotide attached to the capture substrate. In an embodiment, the second
member ses (e.g., from 5’ to 3’) one, two, or all of: (i) a sequence that is complementary to at
least a portion of the first member; (ii) a universal priming sequence (e.g., for PCR amplification or
next-generation sequencing); and (iii) a sequence complementary to a target sequence, e.g., a GCVRS
andior a DCVRS. In an embodiment, the universal priming ce is identical, or substantially
cal, to the sequence that is complementary to at least a portion of the first . In another
ment, the universal g sequence is different from the sequence that is complementary to
at least a portion of the first member. In an embodiment, the second member comprises a sequence
for homologous recombination (e.g., in a yeast or mammalian cell).
In an embodiment, at least one primer comprises a sequence encoding at least a portion of a
linker sequence, or a complementary ce f. In an embodiment, the primer that comprises
a sequence encoding at least a portion of a linker sequence, or a complementary sequence thereof, is
phosphorylated, e.g., 5’ phosphorylated. Without wishing to be bound by theory, it is believed that in
an embodiment, any sequence with the general properties of flexibility (e.g., facilitated by glycine)
and hydrophilicity can work effectively in accordance with the methods described . ary
linkers can generally have overrepresentation of one or more of Gly, Ser, Thr, or Ala and
underrepresentation of hydrophobic residues, e.g., one or more of Trp, Tyr, Phe, Cys, Met, Leu, or 116.
The length of the primer may vary, e.g., 3-50 amino acid residues (e.g., 5-45, 10-40, 15-35, 20-30, 10-
, 10-30, 20-40, or 30-40 amino acid residues). In an embodiment, the linker sequence comprises, or
consists of, ((Gly)m-Ser))n, where m=3, 4, 5, or more and n=1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more. In an
embodiment, the linker sequence ses, or consists of, (Gly-Gly-Gly-Gly-Ser)n, where n=1, 2, 3,
4, 5, 6, 7, 8, 9, 10, or more.
In an embodiment, the primer is a primer bed herein, e.g., in Examples.
In an embodiment, the reverse transcription, the amplification, or both, occurs in a solution in
the isolated production reaction site, e.g., production micro-chamber. In an embodiment, the reverse
transcription, the amplification, or both, does not occur on the substrate (e.g., bead). For example, the
reverse transcription, the amplification, or both, can occur on in a solution within a droplet.
In an ment, the GC ds cDNA comprises a 5’ overhang, e.g., a 5’ overhang that is
capable of hybridizing to an oligonucleotide attached to a e substrate. In an embodiment, the
GC ds cDNA comprises a blunt end, e.g., a blunt end comprising a 5’ phosphate. In an embodiment,
the DC ds cDNA comprises a 5’ overhang, e.g., a 5’ overhang that is e of hybridizing to an
oligonucleotide attached to a capture ate. In an embodiment, the DC ds cDNA comprises a
blunt end, e. g., a blunt end comprising a 5’ phosphate. In an embodiment, the GC ds cDNA and the
DC ds cDNA comprise sticky ends, e.g., both have 5’ overhangs.
In an embodiment, the GC strand and the DC strand are covalently , e.g., ligated, to
produce a single stranded nucleic acid sequence, wherein the GC and DC strands are both sense
strands or both antisense s. In an embodiment, a red GC strand of the GC ds cDNA to a
denatured DC strand of the DC ds cDNA are covalently linked, e.g., ligated, wherein the GC and DC
strands are both sense s or both antisense strands. In an embodiment, the GC strand is present
in the GC ds cDNA and the DC strand is present in the DC ds cDNA, and n the GC ds cDNA
and the DC ds cDNA are covalently linked, e.g., ligated, e.g., to produce a double stranded nucleic
acid sequence.
In an embodiment, the covalent linking, e.g., ligation, occurs in the isolated production
reaction site. In an embodiment, the isolated production reaction site, e.g., a production micro-
r, or the isolated linkage reaction site, e.g., a linkage micro-chamber, comprises a reagent that
is capable of covalently linking, e.g., ligating, the GC and DC strands or the GC and DC ds cDNAs.
In an embodiment, the isolated production reaction site, e.g., a production micro-chamber comprises
an enzyme that covalently couples the GC and DC strands or the GC and DC ds cDNAs. In an
embodiment, the enzyme is a ligase, e. g., a thermal stable ligase. In an embodiment, the covalent
linking comprises ligase cycling.
In an embodiment, the covalent linking, e. 3., ligation, occurs in a site different from the
isolated tion reaction site, e.g., occurs in an isolated e reaction site, e.g., a linkage micro-
chamber. In an embodiment, the GC strand and the DC strand are transferred from the isolated
production site to the isolated linkage reaction site, e.g., a linkage micro-chamber, and the covalent
linking occurs in the isolated e reaction site, e.g., a linkage micro-chamber. In an embodiment,
the isolated linkage reaction site, e.g., a linkage micro-chamber, comprises a reagent that is capable of
covalently g, e.g., ligating, the GC and DC strands or the GC and DC ds cDNAs. In an
embodiment, the ed linkage reaction site, e. g., a linkage micro-chamber, ses an enzyme
that covalently couples the GC and DC strands or the GC and DC ds cDNAs. In an embodiment, the
enzyme is a ligase, e.g., a thermal stable ligase. In an embodiment, the covalent g comprises
ligase cycling.
In an embodiment, the covalent linking, e. 3., ligation, comprises: (a) heating the isolated
linkage reaction site, e. g., the linkage micro-chamber, under conditions (e.g., at 950C) that allow
denaturation of the GC strand and the DC strand; (b) cooling the isolated linkage reaction site, e.g.,
the linkage micro-chamber, under conditions (e.g., at 50-65°C) that allow hybridization of the splint
ucleotide to the GC strand and the DC strand; (c) maintaining the isolated linkage reaction site,
e.g., the linkage micro-chamber, under conditions (e.g., at 45-650C) that allow ligation of the GC
strand and the DC strand (e.g., formation of phosphodiester bond between the GC strand and the DC
); and (d) repeating steps (a), (b), and (c) sequentially for 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50,
or more cycles.
In an embodiment, the GC strand and the DC strand are covalently linked, e.g., ligated, in the
presence of a splint oligonucleotide. In an ment, the splint oligonucleotide is hybridized to a
sequence comprising the junction of the GC strand and the DC strand, or a sequence mentary
f, and forms a duplexed region at the site of ligation. In an embodiment, the splint
oligonucleotide comprises a modification (e.g., an NH2 group) that inhibits DNA synthesis, e.g., by a
DNA polymerase. In an embodiment, the ation is at the 3’ end of the splint oligonucleotide.
In an embodiment, a strand complimentary to the covalently linked, e.g., ligated, GC and DC
strands is produced by amplification.
In an embodiment, the method, e.g., the step of covalent linkage, does not include a step of
p extension polymerase chain reaction (OE-PCR), also known as splicing by p extension
or splicing by overhang extension (SOE) PCR.
In an embodiment, the method further comprises, prior to acquiring the isolated production
reaction site, e.g., a tion micro-chamber, acquiring an mRNA loaded e substrate.
In an embodiment, ing the mRNA loaded capture substrate comprising: a) acquiring an
isolated cell reaction site, e.g., a cell isolation micro-chamber, comprising: i) a cell; and ii) a capture
substrate capable of binding a first mRNA encoding a GCVR from the cell and a second mRNA
encoding a DCVR from the cell; and b) maintaining the isolated cell reaction site, e.g., the cell
isolation micro-chamber, under conditions that allow lysis of the cell and binding of the capture
substrate with the first mRNA and the second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell on site, e.g., cell isolation chamber, does not include a nucleic
acid encoding a GCVR or a DCVR from a cell other than the cell (e.g., a different cell).
In an embodiment, the isolated cell reaction site, e.g., cell isolation micro-chamber, comprises
a lysing reagent, e.g., a detergent. In an embodiment, the cell is lysed by heat or an enzyme. In an
embodiment, the capture substrate comprises a moiety (e.g., an oligonucleotide) which binds mRNA,
e. g., an oligo(dT).
In an embodiment, the method further comprises releasing the mRNA loaded capture
substrate from the isolated cell reaction site, e.g., the cell isolation micro-chamber. In an
embodiment, the releasing step is med in the presence of a A) or poly(dT)
oligonucleotide, e.g., to reduce cross-binding of non-captured mRNA.
In an embodiment, the mRNA loaded capture substrate is transferred from the isolated cell
reaction site, e. g., the cell ion chamber, to the isolated production on site, e.g., the
production micro-chamber.
In an embodiment, the method r comprises releasing the nucleic acid sequence from the
isolated production reaction site, e.g., the production micro-chamber. In an embodiment, the method
further comprises ying the nucleic acid sequence. In an embodiment, amplification of the
nucleic acid sequence occurs outside the isolated production reaction site, e.g., the production micro-
chamber, e. g., after the nucleic acid is released from the isolated production reaction site, 6.55., the
production micro-chamber. In an ment, amplification of the nucleic acid sequence occurs at
the isolated production reaction site, e.g., the production micro-chamber.
In an embodiment, the method further comprises sequencing all or a portion of the nucleic
acid sequence.
In an embodiment, the method further comprises inserting all or a portion of nucleic acid
ce into a vector. In an embodiment, the vector supplies an additional GC element or DC
element not ed in the c acid sequence. In an embodiment, the method further comprises
expressing the vector.
In an embodiment, the method further comprises expressing the nucleic acid sequence to
produce a ptide comprising a segment that encodes a GC element of the GCVR, e.g., a
GCVRS, and a segment that encodes a DC element of the DCVR, e. g., a DCVRS. In an embodiment,
the DC element is N-terminal to the GC element in the polypeptide. In an embodiment, the GC
element is C-terminal to the DC element in the ptide.
In an embodiment, the method further comprises contacting the ptide with an antigen.
In an embodiment, the method r comprises determining if the polypeptide binds the antigen, in
vitro, ex vivo, or in viva, e.g., by a method or assay described herein.
In an embodiment, the disclosure features a method of making a nucleic acid sequence
comprising a sequence that encodes a TCR 7 chain element (GC element) of TCR 7 chain variable
region (GCVR) and a TCR 5 chain element (DC element) of a TCR 5 chain variable region (DCVR),
and n the GCVR and DCVR are matched, comprising:
a) acquiring an ed cell on site (e.g., an isolated cell reaction site described herein),
e.g., a cell isolation micro-chamber, comprising: i) a cell (e.g., a cell described herein); and ii) a
capture substrate (e.g., a capture substrate described herein) capable of g a first mRNA
encoding a GCVR from the cell and a second mRNA encoding a DCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form an mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation chamber, does not include a
nucleic acid encoding a GCVR or a DCVR from a cell other than the cell (e.g., a different cell);
c) contacting the mRNA loaded capture substrate with a reaction mixture, e.g., a reaction
mixture sing reverse transcriptase, that uses the loaded mRNA as a template to make cDNA
(this can occur, e.g., in the isolated cell reaction site, in an isolated production on site, or in
neither, e.g., not in an isolated reaction site);
d) acquiring an isolated production reaction site (e.g., an isolated tion reaction site
described herein), e.g., a production micro-chamber, comprising: i) a TCR 7 chain (GC) strand,
wherein the GC strand is a strand of a TCR 7 chain double-stranded cDNA (GC ds cDNA) comprising
a segment that encodes a GC t of the GCVR from the cell, e.g., a TCR V chain variable region
sequence ); and ii) a TCR 5 chain (DC) , wherein the DC strand is a strand of a TCR 5
chain double-stranded cDNA (DC ds cDNA) comprising a segment that encodes a DC element of the
DCVR from the cell, e. g., a TCR 5 chain variable region sequence (DCVRS),
n the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding a GCVR or a DCVR from a cell other than the cell (e.g., a different
cell); and
e) covalent linking, e.g., ligation, of the GC strand to the DC strand.
In an embodiment, one or more (e.g., two, three, four, or all) of the steps a)-e) are performed
in accordance with a method described herein. In an embodiment, each of the steps a)-e) is performed
in accordance with a method described herein.
In an aspect, the disclosure features a method of making a nucleic acid sequence comprising a
sequence that encodes a TCR V chain element (GC element) of a TCR 7 chain variable region
(GCVR) and a TCR 5 chain element (DC element) of a TCR 5 chain variable region (DCVR), and
wherein the GCVR and DCVR are d, comprising:
a) acquiring an isolated cell reaction site (e.g., an isolated cell reaction site described herein),
e.g., a cell isolation micro-chamber, comprising: i) a cell (e.g., a cell described herein); and ii) a
e ate (e.g., a capture substrate described herein) capable of binding a first mRNA
encoding a GCVR from the cell and a second mRNA encoding a DCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a
c acid encoding a GCVR or a DCVR from a cell other than the cell (e.g., a different cell);
c) acquiring an isolated production reaction site (e.g., an isolated tion reaction site
described herein), e.g., a production micro-chamber, comprises: contacting the mRNA loaded capture
substrate with a reaction mixture, e.g., a reaction mixture comprising reverse transcriptase, that uses
the loaded mRNA as a template, to produce: a first double-stranded cDNA (ds cDNA) comprising a
strand that is complementary to a first mRNA that encodes a GCVR from a cell; and a second ds
cDNA comprising a strand complementary to a second mRNA encoding a DCVR from the cell (the
cDNA loaded e substrate);
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
e a c acid encoding a GCVR or a DCVR from a cell other than the cell (e.g., a different
cell).
d) maintaining the isolated production reaction site, e.g., the tion micro-chamber,
under ions that allow amplification of the first and second ds cDNAs, to produce: a plurality of
GC ds cDNAs comprising a segment that s a GC element of the GCVR from the cell, e.g., a
GCVRS; and a ity of DC ds cDNAs comprising a segment that encodes a DC element of the
DCVR from the cell, e.g., a DCVRS;
e) acquiring an isolated linkage reaction site (e.g., an isolated linkage reaction site described
herein), e.g., a linkage micro-chamber, comprising: covalent linking, e.g., ligation, of a strand of the
GC ds cDNA (GC strand) to a strand of the DC ds cDNA (DC strand), wherein the GC and DC
strands are both sense strands or nse strands; and
f) amplifying the covalently linked, e.g., ligated, GC and DC strands.
In an embodiment, one or more (e.g., two, three, four, five, or all) of the steps a)-f) are
med in accordance with a method described herein. In an embodiment, each of the steps a)-f) is
performed in accordance with a method described herein.
WO 19402
In an aspect, the disclosure features a method of making a library comprising a plurality of
unique members, the method comprising:
making the plurality of members, n each of the members comprises a ce that
encodes a TCR V chain element (GC element) of a TCR y chain le region (GCVR) and a TCR 6
chain element (DC element) of a TCR 5 chain variable region (DCVR), and wherein the GCVR and
DCVR are matched, made by a method bed herein,
wherein each unique nucleic acid sequence of the plurality comprises a GC element and a DC
element from a different unique cell (e.g., a cell described herein),
thereby making a library comprising a plurality of unique members.
In an embodiment, the plurality of unique members comprises at least 104, 105, 106, 107, 108,
or 109 unique members. In an embodiment, the plurality of unique members comprises 104 to 109, 104
to 108, 104 to 107, 104 to 106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 108, 106 to
107, 104 to 105, 105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique members. In an embodiment, at
least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the s in the library are unique
members (which encode matched GC element and DC element sequences). In an embodiment, less
than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the s in the library are unique members
(which encode matched GC element and DC element sequences).
In an aspect, the disclosure features a library comprising a plurality of unique members,
wherein,
i) each unique member of the ity ses a segment that encodes a GC element, e. g.,
a GCVRS, and a segment that encodes a DC element, e.g., a DCVRS, wherein the GC element and
the DC element in each unique member is matched;
ii) each unique member of the plurality comprises a segment that encodes an GC element,
e.g., a GCVRS, and a segment that encodes a DC element, e.g., a DCVRS, from a different unique
cell; and
iii) the library comprises one or more of the ing properties:
a) the library is made by a method described ;
b) the plurality of unique s comprises at least 104, 105, 106, 107, 108, or 109 unique
nucleic acid sequences;
c) the plurality of unique members comprises 104 to 109, 104 to 108, 104 to 107, 104 to 106, 104
to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 103, 106 to 107, 104 to 105, 105 to 106, 106 to
107, 107 to 108, or 108 to 109 unique members;
d) at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the members in the
library are unique members (which encode matched GC element and DC element sequences); or
e) less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the members in the library are
unique members (which encode matched GC element and DC element sequences).
In an embodiment, each unique member of the plurality is configured such that, when
expressed, the GC element, e.g., the GCVRS, and the DC element, e.g., the DCVRS, form a
functional antigen binding molecule, e.g., a single chain or a complex of a TCR 7 chain and a 5 chain.
In an embodiment, the library is a display y. In an embodiment, each of the members of
the plurality further s a polypeptide that results in display of the member on the surface of a
y entity. In an embodiment, the y is a phage display library. In an embodiment, the
library is a yeast y library. In an embodiment, the library is a mammalian display library.
In an aspect, the disclosure features a method of making a binding ptide (e.g., a
polypeptide comprising a GC element and a DC element), the method comprising: a) acquiring a
library bed herein, e.g., by a method bed herein; and b) sing a polypeptide encoded
by a unique nucleic acid of the library.
In an embodiment, the method further comprises contacting the polypeptide with an n.
In an ment, the method further ses retrieving (e.g., isolating or purifying) the nucleic
acid that encodes a polypeptide that binds the antigen.
In an aspect, the disclosure features an isolated production reaction site, e.g., a production
micro-chamber, which is an isolated production reaction site described herein (e.g., comprising a
nucleic acid encoding a GCVR and a nucleic acid encoding a DCVR, wherein the GCVR and the
DCVR are matched).
In an embodiment, the isolated production reaction site, e.g., a production micro-chamber,
does not include a nucleic acid encoding a GCVR or a DCVR from a different cell.
In an embodiment, the isolated production reaction site, e.g., a production micro-chamber,
comprises one, two, or all of: (i) one or more primers ic to V gene sequences of the GC and DC;
(ii) one or more primers c to overhangs introduced onto the GC and DC cDNAs; or (iii) one or
more primers comprising a first member, a second member, and a third member comprising a
nucleotide modification (e.g., a spacer) located between the first and second members, wherein the
first member is capable of annealing with the second member of the same primer or a different
primer, e.g., g a structure comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,
16, 17, 18, 19, 20, more basepairs.
In an embodiment, the isolated production reaction site, e.g., a production micro-chamber,
does not comprise a reagent that can covalently link nucleic acids, e.g., a , e.g., a thermostable
1i gase. In another embodiment, the isolated production reaction site, e. g., a production micro-
chamber, comprises a reagent that can ntly link nucleic acids, e.g., a ligase, e.g., a thermostable
ligase.
In an aspect, the disclosure features a self-annealing oligonucleotide comprising a first
member, a second , and third member comprising a nucleotide modification (e. g., a spacer)
located between the first and second members, wherein the first member is capable of annealing with
the second member of the same oligonucleotide (e.g., for a method of making a nucleic acid sequence
comprising a sequence that encodes a GC element of a GCVR and a DC element of a DCVR, wherein
the GCVR and DCVR are d).
In an embodiment, the first and second members are capable of g a hairpin structure
sing a duplex region of 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more
basepairs. In an embodiment, the first member is 5-40 nucleotides, e.g., 5-10, 5-20, 5-30, 30-40, 20-
40, 10-30, 10-30, or 15-25 nucleotides, in length. In an embodiment, the second member is 5-40
nucleotides, e.g., 5-10, 5-20, 5-30, 30-40, 20-40, 10-30, 10-30, or 15-25 nucleotides, in length.
In an embodiment, the spacer is a spacer described , e.g., a flexible spacer or a PEG
spacer.
In an embodiment, the first member comprises a sequence that is complementary to the
sequence of an oligonucleotide attached to a capture substrate.
In an ment, the second member comprises (e.g., from 5’ to 3’) one, two, or all of: (i) a
sequence that is mentary to at least a portion of the first member; (ii) a universal priming
sequence (e.g., for PCR amplification or next-generation sequencing); and (iii) a sequence
complementary to a target sequence, e.g., a GCVRS and/or a DCVRS. In an embodiment, the
universal priming ce is identical, or substantially identical, to the sequence that is
complementary to at least a portion of the first member. In another embodiment, the universal
priming sequence is different from the ce that is mentary to at least a portion of the first
member. In an embodiment, the second member comprises a sequence for homologous
recombination (e.g., in a yeast or mammalian cell).
In an aspect, the sure features an isolated linkage reaction site, e.g., a linkage micro-
chamber, which is an isolated linkage reaction site described herein (e.g., comprising a nucleic acid
encoding a GCVR and a nucleic acid encoding a DCVR, wherein the GCVR and the DCVR are
matched).
In an embodiment, the isolated linkage reaction site, e.g., a linkage micro-chamber, does not
include a nucleic acid encoding a GCVR or a DCVR from a different cell.
In an ment, the isolated linkage reaction site, 6. g., a linkage micro-chamber, comprises
a splint oligonucleotide (e.g., a splint oligonucleotide described herein) that is capable of hybridizing
to a sequence sing the junction of the GC strand and the DC strand, or a ce
complementary thereof, to form a duplexed region at the site of ligation.
In an embodiment, the isolated linkage reaction site, e.g., a e micro-chamber, comprises
a reagent that can covalently link nucleic acids, e.g., a ligase, e.g., a thermostable ligase.
BRIEF DESCRIPTION OF THE DRAWINGS
depicts a number of ways of making nucleic acid ce comprising a sequence that
encodes a heavy chain element (HC element) of an antibody heavy chain variable region (HCVR) and
a light chain element (LC t) of an antibody light chain variable region (LCVR), and wherein
the HCVR and LCVR are matched. The A1, B1 and C2 boxes indicate steps occurring in an isolated
reaction site, particularly, in an isolated cell reaction site. The C3, D1, D2, D3, D4, D5 and D6 boxes
indicate steps occurring in an isolated reaction site, particularly, in an isolated production reaction
site. The E1, E2 and E3 boxes indicate steps occurring in an isolated reaction site, ularly, in an
ed linkage on site. The C1 box indicates steps that need not occur in an isolated reaction
site. As is discussed in the text, the isolated reaction sites are free of nucleic acid that would result in
a ched HC and LC element.
FIGS. 2A-2D are a series of diagrams showing an exemplary method of making a nucleic acid
comprising a sequence that encodes a heavy chain element (HC element) of an dy heavy chain
variable region (HCVR) and a light chain element (LC element) of an antibody light chain variable
region (LCVR), and wherein the HCVR and LCVR are matched. In , a cell (e.g., an immune
cell, such as a B cell) is lysed and mRNAs encoding an HCVR and a matched LCVR are captured on
a bead. In , captured mRNAs are ted to cDNA by reverse transcription followed by
amplification by DNA polymerase (PCR) to create cDNA beads comprising matched pairs of HCVR
and LCVR cDNAs. A self-annealing primer (e.g., a primer comprising a first member and a second
member capable of hybridizing to the first member, with the first and second members separated by a
spacer, e.g., a PEG spacer, and further comprising a sequence capable of hybridizing to an HCVR or
LCVR sequence) can be used for the reverse transcription on and/or DNA polymerase
amplification. In , matched LCVR and HCVR cDNA products can be fused using a ligase
cycling reaction, in which matched pairs of LCVRs and HCVRs are brought together using a splint
oligo comprising sequences capable of hybridizing to an end of each of the LCVR or HCVR
sequences (e.g., the 3’ terminus of the LCVR and the 5’ terminus of the HCVR). In , the
fused LCVR/HCVR product can be amplified, e.g., by PCR.
is a polyacrylamide gel electrophoresis (PAGE) image g that Taq ligase and Ampligase
thermostable ligase (Amp; Lucigen) were capable of efficiently g VH and VL product.
FIGS. 4A-4B are gel electrophoresis images showing that natively paired, linked VH + VL products
for each of antibodies 4G2 and 9E10 were successfully produced by ligase cycling. In ,
denaturing PAGE of ligase g products showed that ligase-containing reactions yielded the
linked VH + VL products for each of 4G2 and 9E10, as well as the individual VH and VL
polynucleotides. The linked VH + VL ts were not detected in reactions lacking ligase. In FIG.
WO 19402
4B, agarose gel electrophoresis of bulk PCR re-amplification products showed that native pairing was
retained for when VH-VL linked polynucleotides for 4G2 and 9E10 were mixed in the PCR reaction.
FIGS. 5A-SB are a graph and diagram showing efficient and specific PCR product capture using a
self-annealing primer. In , a series of forward PCR primer designs were tested for their
capacity to capture PCR product, ing (1) a VL primer comprising a spacer and with 5’ sequence
complementary to oligo on bead and 3’ sequence that is complementary to VL template sequence, (2)
a VL primer lacking a spacer and with 5’ sequence complementary to oligo on bead and 3’ sequence
that is complementary to VL template sequence, (3) a VL primer lacking 5’ sequence mentary
to oligo on bead and 3’ ce that is complementary to VL template sequence, and (4) a VH
primer with similar design as in (l) but with 3’-end having sequence complementary to VH template
(for DNA polymerase extension). In , the VL primer comprising a spacer was used for
ent and specific PCR capture of VL oligo, VH oligo, and VH+VL oligo. Of the remaining
primers, only the VH primer was capable of capturing any of the oligos (specifically, the VH oligo
and VH+VL oligo).
is an agarose gel electrophoresis image showing that natively paired VH-VL products could be
produced in drops from nucleic acids ed from cells expressing the 4G2 antibody. NTC = sample
in which the entire droplet workflow was performed but no cells were included; PCR NTC = no-
template control.
FIGS. 7A-7B are a series of graphs showing that self-annealing primers (in ) can t
PCR product capture competition at high levels of unused primer, whereas non-self—annealing primers
(in ) can only do so at low levels of unused primer.
DETAILED DESCRIPTION
Disclosed herein are polypeptides (e.g., antibody molecules or T cell receptor molecules) that
bind to a target molecule or cell, e.g., a human protein or cell, with high affinity and specificity. In an
embodiment, the polypeptide is a g polypeptide. In an embodiment, the binding polypeptide is
an antibody molecule. In an ment, the binding polypeptide is a TCR molecule (e.g., a soluble
TCR molecule). In an embodiment, libraries of the polypeptides, methods for making the
polypeptides or libraries, c acid molecules encoding the polypeptides, expression vectors, host
cells, and compositions (e.g., pharmaceutical compositions), kits, ners, are also provided. The
s described herein are useful for making or selecting functional polypeptides that contain two
or more chains that are lly matched or paired. The polypeptides (e.g., antibody molecules or T
cell receptor molecules) sed herein can be used (alone or in combination with other agents or
therapeutic modalities) to treat, prevent and/or diagnose disorders, such as disorders and conditions
disclosed herein.
Without wishing to be bound by theory, it is ed that the methods described herein can
facilitate, e. g., high-throughput phenotypic (e.g., binding) screening of ns of B-cellfplasma cell
antibodies, and antibody discovery from B-cells derived from different species, including, but not
d to, human, mouse, rat, rabbit, or chicken. For example, the only requirement can be
dge of primers to appropriately amplify VH and VL sequences from that species.
Since the workflow bed herein is amenable to use in any species, it can significantly
improve ability to discover diverse binding polypeptides (e. g., antibodies) to target antigens (post
immunization/vaccination), as each species ps different types of binding polypeptides (e.g.,
antibodies) to an antigen. Immune tolerance issues (e.g., to a target epitope) can be better overcome
by using a species which lacks the target antigen or has significant amino acid differences to the target
antigen, e.g., chicken has d nce to human antigens/epitopes than human or mouse does to
human antigens/epitopes.
The methods described herein can facilitate making a ‘phenotypic copy’ of an dy
repertoire in yeast, which are rugged and can be regrown. This tates rigorous and repeated
testing of the antibody repertoire, unlike when using primary B-cells, which are sensitive, do no
survive long in vitro, and cannot survive rigorous antibody/BCR binding characterizations.
Other methods to generate ly paired VH-VL sequences in droplets can use splicing by
overlap extension with DNA polymerase (PCR) to link the DNA, which may have limitations with
specificity and can result in heterogeneous products of divergent sizes due to imprecise linking. The
ligation methods described here do not suffer from such issues.
Additionally, droplet methods using splicing by overlap extension PCR suffer from an
inherent limitation in which any PCR products not fused within drops have the potential to become
fused during non-drop PCR amplification due to the common ed sequence between VH and
VL. Fusion occurring outside of drops leads to non-native pairing, as chains are not
compartmentalized. For the exemplary ligation workflow described herein, there is no need to add
common sequence to VH and VL, and therefore this issue is precluded from occurring.
Such PCR amplification can lead to significantly biased representation of divergent
sequences, as some ces amplify more efficiently than others, which can lead to dramatic
differences after the ntial amplification which occurs in PCR. The workflow described herein
reduces this issue by having PCR products captured onto a bead. For example, if a cell’s VH and VL
sequences are amplified very well or poorly, a r amount of product will be captured onto the
bead. Thereby, there is a more even entation of antibody sequences in the final library, relative
to methods that omit this step and perform linking by splicing by overlap extension PCR.
Definitions
An “HC le region,” as that term is used herein, refers to a polypeptide comprising
heavy chain CDRs 1, 2 and 3 and heavy chain FW regions 1, 2, 3, and 4.
An “LC variable region,” as that term is used herein, refers to a polypeptide comprising light
chain CDRs 1, 2 and 3 and light chain FW regions 1, 2, 3, and 4.
A “heavy chain variable region sequence,” or “HCVRS,” as that term is used herein, refers to
a polypeptide comprising sufficient sequence from heavy chain CDRs and sufficient sequence from
heavy chain FW regions, to allow binding of antigen. In embodiments the HCVRS can assemble with
a light chain variable region, and, e.g., bind antigen. In an embodiment, a HCVRS comprises
sufficient sequence from heavy chain CDRs 1, 2, and 3,and sufficient sequence from heavy chain FW
regions, e.g., heavy chain FW regions 1, 2, 3, and 4, to allow binding of antigen. In an ment, a
HCVRS ses heavy chain CDRs 1, 2, and 3, and sufficient sequence from heavy chain FW
regions, e.g., heavy chain FW s 1, 2, 3, and 4, to complex with a light chain variable region and
to allow binding of antigen.
A “light chain variable region sequence,” or “LCVRS,” as that term is used herein, refers to a
polypeptide comprising sufficient sequence from light chain CDRs and sufficient sequence from light
chain FW regions, to allow binding of antigen. In embodiments the LCVRS can assemble with a
heavy chain le region, and, e.g., bind antigen. In an embodiment, a LCVRS comprises
sufficient sequence from light chain CDRs l, 2, and 3,and ient sequence from light chain FW
regions, e.g., light chain FW regions 1, 2, 3, and 4, to allow binding of antigen. In an embodiment, a
LCVRS comprises light chain CDRs 1, 2, and 3, and sufficient sequence from light chain FW regions,
e.g., light chain FW regions 1, 2, 3, and 4, to x with a heavy chain variable region and to allow
binding of antigen.
“Element” of an LC or HC variable region, as that term is used herein, refers to a sequence
that encodes at least one amino acid. In an embodiment, an element comprises a CDR. In an
embodiment an element comprises a FW region. In an embodiment, and element comprises a CDR
and a FW region. In an embodiment an element comprises a HCVRS or a LCVRS. In an
embodiment, the element comprises at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 amino acid
residues.
A “micro-chamber,” as that term is used , refers to a compartment that is dimensioned,
e.g., is sufficiently small, such that upon formation it ns a single cell, or the content from a
single cell. In an ment, the micro-chamber has a volume of that is 10 to 10,000 times r
of a cell that it contains. In an embodiment, the micro-chamber has a volume of 20 pL. In an
embodiment, the micro-chamber has a maximum dimension of 100 nL. In an embodiment the micro-
chamber comprises a droplet of liquid. In ment, the micro-chamber comprises a droplet of a
first liquid disposed in an immiscible media, e.g., a gas or second . In an embodiment, the
micro-chamber comprises a droplet of a first liquid, e. g., a lysis buffer or a PCR reaction ,
formed by dispersing the first liquid in an immiscible second liquid, e.g., a fluorinated oil. In an
embodiment, the micro-chamber comprises a substrate and a nce other than the substrate, e.g., a
solution. In an embodiment, the droplet comprises a substrate (e.g., a capture substrate, e.g., a bead)
and a substance other than the substrate, e.g., a solution.
“Acquiring,” as that term is used herein, refers to possession of provision of an entity, e.g., a
physical entity or data. Acquiring a physical entity includes making or manufacturing a physical
entity tly acquiring) as well as receiving a physical entity from another party or source
(indirectly acquiring). Acquiring a data or a value includes generating the data or value (directly
acquiring) as well as ing the data or value from another party or source (indirectly acquiring).
“Matched,” as that term is used herein in connection with a heavy chain variable region and a
light chain variable region, means they are from the same cell. With respect to an t of a light
chain le region and an element of a heavy chain variable region it means that the light chain
le region and the heavy chain variable region from which the elements are derived are from the
same cell.
An “isolated reaction site,” as that term is used here, refers to a site, e.g., a location on a
substrate, a micro chamber, or a well on a substrate, which allows for sufficient separation between a
first loaded capture ate and a second loaded capture substrate, or generally, from HC or LC (or
at chain or [3 chain, or 7 chain or 6 chain) encoding nucleic acid of another cell, such that the first
loaded capture substrate is not contaminated with nucleic acid encoding a HC or LC (or 0t chain or [5
chain, or y chain or 5 chain) from another cell. In an embodiment an isolated reaction site provides
sufficient separation between a first mRNA loaded capture substrate and a second mRNA loaded
e substrate, or generally, from LC or HC encoding nucleic acid of another cell, that the first
loaded mRNA capture substrate is not inated with nucleic acid, e. g., mRNA, encoding an HC
or LC (or a chain or [3 chain, or 7 chain or 6 chain) from another cell. In an embodiment an ed
reaction site provides sufficient separation n a first cDNA loaded capture substrate and a
second cDNA loaded capture substrate, or generally, from HC or LC (or 0. chain or [3 chain, or y chain
or 8 chain) encoding nucleic acid of another cell, that the first loaded cDNA capture substrate is not
contaminated with nucleic acid, e.g., cDNA, encoding a HC or LC (or 0L chain or [3 chain, or 7 chain or
5 chain) from another cell. Separation can be provided, e.g., by sufficient distance n isolated
reaction sites on a substrate; by configuring the isolated reaction sites such that they are not in fluid
connection, or by formation of an immiscible barrier n a volume or chamber and the
environment. In an embodiment, the isolated reaction site comprises a substrate and a substance other
than the substrate, e.g., a solution.
“Complimentary,” as that term is used herein, refers to sequences which can form Watson-
Crick pairing. When a first sequence is complementary with a second ce it can be
mentary to the entire second sequence or to less than all of the second sequence.
A “display entity,” as that term is used herein, refers to an entity, e. g., a phage or cell, e. g., a
yeast cell, which es a gene that encodes a polypeptide.
An “AC variable region,” as that term is used herein, refers to a polypeptide comprising TCR
0t chain CDRs l, 2 and 3 and 0t chain FW regions 1, 2, 3, and 4.
A “BC le region,” as that term is used herein, refers to a ptide comprising [3
chain CDRs 1, 2 and 3 and [3 chain FW regions 1, 2, 3, and 4.
A “GC variable region,” as that term is used , refers to a polypeptide comprising TCR V
chain CDRs 1, 2 and 3 and 7 chain FW regions 1, 2, 3, and 4.
A “DC variable region,” as that term is used herein, refers to a polypeptide comprising 5
chain CDRs l, 2 and 3 and 5 chain FW regions 1, 2, 3, and 4.
An “(1 chain variable region sequence,” or “ACVRS,” as that term is used herein, refers to a
polypeptide comprising sufficient sequence from 0t chain CDRs and sufficient ce from 0. chain
FW regions, to allow g of antigen. In ments the ACVRS can assemble with a [3 chain
variable region, and, e.g., bind antigen. In an embodiment, a ACVRS comprises sufficient sequence
from or chain CDRs 1, 2, and 3, and sufficient sequence from or chain FW s, e.g., or chain FW
regions 1, 2, 3, and 4, to allow binding of antigen. In an embodiment, an ACVRS ses 0. chain
CDRs 1, 2, and 3, and sufficient sequence from or chain FW regions, e.g., 0. chain FW regions 1, 2, 3,
and 4, to complex with a [3 chain variable region and to allow binding of antigen.
A “[3 chain variable region sequence,” or “BCVRS,” as that term is used herein, refers to a
polypeptide comprising sufficient sequence from [3 chain CDRs and sufficient sequence from [3 chain
FW regions, to allow binding of n. In embodiments the BCVRS can assemble with an 0. chain
variable region, and, e.g., bind antigen. In an embodiment, a BCVRS comprises sufficient sequence
from [3 chain CDRs 1, 2, and 3, and sufficient sequence from [5 chain FW regions, e.g., [3 chain FW
regions 1, 2, 3, and 4, to allow binding of antigen. In an embodiment, a BCVRS comprises [3 chain
CDRs 1, 2, and 3, and sufficient sequence from [3 chain FW s, e.g., [3 chain FW regions 1, 2, 3,
and 4, to complex with an a chain variable region and to allow binding of antigen.
A “7 chain variable region sequence,” or “GCVRS,” as that term is used herein, refers to a
polypeptide comprising sufficient sequence from 7 chain CDRs and sufficient sequence from 7 chain
FW regions, to allow binding of antigen. In ments the GCVRS can assemble with a 5 chain
variable region, and, e.g., bind antigen. In an embodiment, a GCVRS comprises sufficient sequence
from 7 chain CDRs 1, 2, and 3, and sufficient sequence from y chain FW regions, e.g., y chain FW
regions 1, 2, 3, and 4, to allow binding of antigen. In an embodiment, a GCVRS comprises 7 chain
CDRs l, 2, and 3, and sufficient sequence from y chain FW regions, e.g., 7 chain FW regions 1, 2, 3,
and 4, to complex with a 5 chain variable region and to allow binding of antigen.
A “5 chain variable region ce,” or “DCVRS,” as that term is used herein, refers to a
polypeptide comprising sufficient sequence from 5 chain CDRs and sufficient sequence from 5 chain
FW s, to allow binding of antigen. In embodiments the DCVRS can assemble with a y chain
variable , and, e.g., bind antigen. In an embodiment, a DCVRS comprises sufficient sequence
from 5 chain CDRs 1, 2, and 3,and sufficient sequence from 5 chain FW regions, e.g., 5 chain FW
regions 1, 2, 3, and 4, to allow binding of antigen. In an embodiment, a DCVRS comprises 5 chain
CDRs 1, 2, and 3, and sufficient sequence from 5 chain FW regions, 6g, 5 chain FW s 1, 2, 3,
and 4, to complex with an or chain variable region and to allow binding of antigen.
“Element” of an 0. chain or [3 chain le region, as that term is used herein, refers to a
sequence that encodes at least one amino acid. In an embodiment, an element comprises a CDR. In
an embodiment an element ses a FW region. In an embodiment, and element comprises a
CDR and a FW region. In an embodiment an element comprises an ACVRS or a BCVRS. In an
embodiment, the t comprises at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 amino acid
“Element” of a 7 chain or 5 chain variable region, as that term is used herein, refers to a
sequence that encodes at least one amino acid. In an embodiment, an element comprises a CDR. In
an embodiment an element comprises a FW region. In an embodiment, and element comprises a
CDR and a FW region. In an embodiment an element comprises a GCVRS or a DCVRS. In an
embodiment, the element ses at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 amino acid
residues.
“Matched,” as that term is used herein in connection with an or chain le region and a [5
chain variable region, means they are from the same cell. With respect to an element of an 0. chain
variable region and an element of a [3 chain variable region it means that the or chain variable region
and the [3 chain variable region from which the elements are derived are from the same cell.
“Matched,” as that term is used herein in connection with a 7 chain variable region and a 5
chain variable region, means they are from the same cell. With respect to an t of a y chain
variable region and an element of a 5 chain variable region it means that the 7 chain variable region
and the 7 chain variable region from which the elements are derived are from the same cell.
As used herein, the articles “a” and “an” refer to one or to more than one (e.g., to at least one)
of the grammatical object of the article.
The term “or” is used herein to mean, and is used interchangeably with, the term “and/or”,
unless context clearly indicates otherwise.
“About” and “approximately” shall generally mean an acceptable degree of error for the
quantity measured given the nature or ion of the measurements. Exemplary degrees of error are
within 20 percent (%), typically, within 10%, and more typically, within 5% of a given value or range
of values.
The compositions and methods disclosed herein ass polypeptides and nucleic acids
having the sequences specified, or sequences substantially identical or similar thereto, e.g., sequences
at least 85%, 90%, 95% identical or higher to the sequence specified.
In the context of an amino acid sequence, the term antially identical” is used herein to
refer to a first amino acid that contains a sufficient or m number of amino acid residues that
are i) identical to, or ii) conservative tutions of aligned amino acid residues in a second amino
acid sequence such that the first and second amino acid sequences can have a common structural
domain and/or common functional activity. For example, amino acid sequences that contain a
common structural domain having at least about 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%,
98% or 99% identity to a reference sequence, e.g., a sequence provided herein.
In the context of nucleotide sequence, the term “substantially identical” is used herein to refer
to a first nucleic acid ce that contains a sufficient or m number of nucleotides that are
identical to aligned nucleotides in a second nucleic acid ce such that the first and second
nucleotide sequences encode a polypeptide having common functional activity, or encode a common
structural polypeptide domain or a common functional polypeptide activity. For example, nucleotide
sequences having at least about 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%
identity to a reference ce, e.g., a sequence provided herein.
The term “functional variant” refers polypeptides that have a substantially identical amino
acid sequence to the naturally-occurring sequence, or are encoded by a substantially identical
nucleotide sequence, and are capable of having one or more activities of the naturally-occurring
sequence.
Calculations of homology or sequence identity between sequences (the terms are used
interchangeably herein) are performed as follows.
To determine the percent identity of two amino acid sequences, or of two nucleic acid
sequences, the sequences are aligned for optimal comparison purposes (e.g., gaps can be uced in
one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and
non-homologous sequences can be disregarded for ison purposes). In a typical embodiment,
the length of a reference sequence aligned for comparison purposes is at least 30%, e.g., at least 40%,
50%, 60%, e.g., at least 70%, 80%, 90%, 100% of the length of the reference sequence. The amino
acid residues or nucleotides at ponding amino acid positions or nucleotide positions are then
compared. When a position in the first ce is occupied by the same amino acid e or
nucleotide as the corresponding position in the second sequence, then the molecules are identical at
that on.
The percent identity between the two sequences is a function of the number of identical
positions shared by the sequences, taking into t the number of gaps, and the length of each gap,
which need to be introduced for optimal ent of the two sequences.
The ison of sequences and determination of percent identity n two sequences
can be accomplished using a mathematical algorithm. In some embodiments, the percent identity
between two amino acid sequences is determined using the Needleman and Wunsch ((1970) J. M01.
Biol. -453) algorithm which has been incorporated into the GAP program in the GCG software
package (available at www.gcg.com), using either a Blossum 62 matrix or a PAM250 matrix, and a
gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of l, 2, 3, 4, 5, or 6. In certain
embodiments, the percent identity between two nucleotide sequences is determined using the GAP
program in the GCG software package (available at www.gcg.com), using a NWSgapdna.CMP matrix
and a gap weight of 40, 50, 60, 70, or 80 and a length weight of l, 2, 3, 4, 5, or 6. One suitable set of
parameters (and the one that should be used unless otherwise specified) are a Blossum 62 scoring
matrix with a gap penalty of 12, a gap extend penalty of 4, and a frameshift gap penalty of 5.
The percent identity between two amino acid or nucleotide sequences can be determined
using the algorithm of E. Meyers and W. Miller ((1989) CABIOS, 4: 11-17) which has been
incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap
length y of 12 and a gap penalty of 4.
The nucleic acid and n ces described herein can be used as a “query sequence” to
perform a search against public ses to, for example, identify other family members or related
sequences. Such searches can be med using the NBLAST and XBLAST programs on 2.0)
of Altschul, et al. (1990) J. Mol. Biol. 215:403-10. BLAST nucleotide searches can be performed
with the NBLAST m, score = 100, wordlength = 12 to obtain nucleotide sequences homologous
to a nucleic acid as described . BLAST protein searches can be med with the XBLAST
program, score = 50, wordlength = 3 to obtain amino acid sequences homologous to protein
molecules described herein. To obtain gapped alignments for comparison purposes, Gapped BLAST
can be utilized as described in Altschul et al., (1997) Nucleic Acids Res. 25 :3389-3402. When
utilizing BLAST and gapped BLAST programs, the default parameters of the respective programs
(e.g., XBLAST and NBLAST) can be used. See www.ncbi.nlm.nih.gov.
As used herein, the term “hybridizes under low stringency, medium stringency, high
ency, or very high stringency conditions” describes conditions for hybridization and washing.
ce for ming hybridization reactions can be found in Current Protocols in Molecular
Biology, John Wiley & Sons, NY. (1989), 63.1-63.6, which is incorporated by reference. Aqueous
and nonaqueous methods are described in that nce and either can be used. Specific
hybridization conditions referred to herein are as s: 1) low stringency hybridization conditions
in 6X sodium chloride/sodium citrate (SSC) at about 45°C, followed by two washes in 0.2X SSC,
0.1% SDS at least at 50°C (the temperature of the washes can be increased to 55°C for low stringency
conditions); 2) medium stringency hybridization conditions in 6X SSC at about 45°C, followed by
one or more washes in 0.2X SSC, 0.1% SDS at 60°C; 3) high stringency hybridization conditions in
6X SSC at about 45°C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 65°C; and
ably 4) very high stringency hybridization conditions are 0.5M sodium phosphate, 7% SDS at
65°C, followed by one or more washes at 0.2X SSC, 1% SDS at 65°C. Very high stringency
conditions 4) are suitable conditions and the ones that should be used unless otherwise specified.
It is understood that the molecules described herein may have additional conservative or non-
essential amino acid substitutions, which do not have a substantial effect on their functions.
The term “amino acid” is intended to embrace all molecules, whether natural or synthetic,
which include both an amino functionality and an acid functionality and capable of being ed in
a polymer of lly-occurring amino acids. Exemplary amino acids include naturally-occurring
amino acids; analogs, tives and congeners thereof; amino acid analogs having variant side
chains; and all stereoisomers of any of any of the foregoing. As used herein the term “amino acid”
includes both the D- or L- optical isomers and peptidomimetics.
A “conservative amino acid substitution” is one in which the amino acid residue is replaced
with an amino acid residue having a similar side chain. es of amino acid residues having
similar side chains have been defined in the art. These families include amino acids with basic side
chains (e.g., lysine, arginine, ine), acidic side chains (e.g., aspartic acid, glutamic acid),
uncharged polar side chains (6.3., glycine, asparagine, ine, serine, threonine, tyrosine,
cysteine), nonpolar side chains (e. g., alanine, valine, leucine, isoleucine, proline, phenylalanine,
methionine, tryptophan), beta-branched side chains (e.g., threonine, valine, cine) and aromatic
side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine).
The terms eptide,93 upeptide” and “protein” (if single chain) are used interchangeably
herein to refer to polymers of amino acids of any length. The polymer may be linear or branched, it
may comprise modified amino acids, and it may be interrupted by ino acids. The terms also
encompass an amino acid polymer that has been modified; for example, disulfide bond formation,
glycosylation, lipidation, acetylation, orylation, or any other lation, such as conjugation
with a labeling ent. The polypeptide can be ed from natural sources, can be a produced
by inant techniques from a eukaryotic or prokaryotic host, or can be a product of synthetic
procedures. In an embodiment, the polypeptide is an antibody molecule. In another embodiment, the
polypeptide is a TCR molecule, e.g., soluble TCR molecule.
The terms “nucleic acid,” “nucleic acid sequence,a, unucleotide sequence,” or “polynucleotide
sequence,” and “polynucleotide” are used interchangeably. They refer to a polymeric form of
nucleotides of any length, either deoxyribonucleotides or ribonucleotides, or analogs thereof. The
polynucleotide may be either single-stranded or double-stranded, and if single-stranded may be the
coding strand or non-coding (antisense) . A polynucleotide may comprise d
nucleotides, such as methylated nucleotides and nucleotide analogs. The sequence of nucleotides may
be interrupted by non-nucleotide components. A polynucleotide may be further modified after
polymerization, such as by conjugation with a labeling component. The nucleic acid may be a
recombinant polynucleotide, or a polynucleotide of genomic, cDNA, semisynthetic, or synthetic
origin which either does not occur in nature or is linked to another polynucleotide in a non-natural
arrangement.
The term “isolated,” as used herein, refers to material that is removed from its original or
native nment (e.g., the natural environment if it is lly occurring). For example, a
lly-occurring polynucleotide or polypeptide present in a living animal is not isolated, but the
same polynucleotide or polypeptide, ted by human intervention from some or all of the co-
existing materials in the l system, is isolated. Such polynucleotides could be part of a vector
andior such polynucleotides or polypeptides could be part of a composition, and still be isolated in
that such vector or composition is not part of the environment in which it is found in .
As used herein, the term “treat,” e.g., a disorder described herein, means that a subject (e.g., a
human) who has a disorder, e.g., a disorder described herein, and/or experiences a symptom of a
disorder, e.g., a disorder described herein, will, in an embodiment, suffer less a severe symptom
and/or recover faster when an antibody molecule is administered than if the antibody molecule were
never stered. Treatment can, e.g., partially or completely, alleviate, rate, relieve, inhibit,
or reduce the severity of, and/or reduce incidence, and optionally, delay onset of, one or more
manifestations of the effects or symptoms, features, and/or causes of the disorder. In an embodiment,
treatment is of a subject who does not exhibit certain signs of the disorder, and/or of a subject who
exhibits only early signs of the disorder. In an ment, treatment is of a subject who exhibits one
or more ished signs of a disorder. In an embodiment, treatment is of a subject diagnosed as
ing from a disorder.
As used herein, the term “prevent,” a disorder, means that a subject (e.g., a human) is less
likely to have the disorder, if the subject receives a polypeptide (e.g., antibody molecule).
Various aspects of the compositions and methods herein are described in further detail below.
Additional definitions are set out throughout the specification.
Libraries of Binding Polypeptides
Disclosed herein are libraries (e.g., y libraries) of binding polypeptides, e. g., antibody
les or T cell receptor molecules, and methods of making libraries of binding polypeptides, e.g.,
antibody molecules or T cell receptor molecules.
In an embodiment, a method described herein links two DNA fragments, such as sequences
encoding an antibody heavy chain variable region (or a portion thereof) and an dy light chain
variable region (or a portion thereof), a TCR or chain (or a portion thereof) and a TCR [3 chain (or a
portion thereof), or a TCR 7 chain (or a portion thereof) and a TCR 5 chain (or a n thereof),
using a ligase-mediated approach.
For example, antibodies are composed of two types of ptide chains, light chain and
heavy chain, each of which are ated from separate mRNA molecules. In order to copy a
functional unit of a ular antibody (or B cell receptor) from a B cell, knowledge of the particular
heavy chain and its cognate light chain must be ined. This is lly med using
methods in which individual clones are in wells of microwell plates, which keeps clones segregated
and the result heavy and light chain sequences thus are known to be paired. Such cloning processes
scale well to B cell numbers compatible with 96- or 384-well plates. However, B cell repertoires in
humans and animals can range from about 106-1011 B cells, many of which are different clones (La,
different BCRs or antibodies). Thus, there is a need to be able to, in an efficient manner, make copies
of millions to billions of B cells which (1) retains native pairing of chains and (2) allows for
functional interrogation of such a large number of unique clones. Such a method can facilitate
making a renewable copy of an antibody oire which can be functionally interrogated by a
variety of methods.
In an embodiment, a method described herein uses one or more (e.g., two, three, or all) of the
following: (1) miniaturized compartmentalization of individual cells (e.g., B cells or T cells) in
droplets (pL to nL volume drops), (2) lysing and PCR amplifying two chains (e.g., antibody VH and
VL, TCR 0t and [3 chains, or TCR V and 5 chains), (3) specifically linking the two , such that
native chain pairing is retained and that a thermostable ligase catalyzes the linking, and (4) amplifying
the linked DNA in a manner that allows for high throughput phenotypic ogation of clones by a
surface display technology, such as yeast or phage display.
The s bed herein can result in a nucleic acid sequence, when expressed, encodes
a functional polypeptide, e.g., a functional antigen binding polypeptide. For e, the HC element
and the LC element (or the AC element and the BC element, or the GC element and the DC element)
are not configured in a head-to-head or tail-to-tail ation. In an embodiment, the HC element and
the LC element (or the AC element and the BC element, or the GC t and the DC element) are
configured in a o-tail orientation. For example, the C-terminus of the LC element (or LCVRS)
is linked, directly or indirectly, with the N-terminus of the HC element (or HCVRS), or the C-
terminus of the HC element (or HCVRS) is linked, directly or indirectly, with the C-terminus of the
LC element (or LCVRS).
Exemplary Workflow
Cells (e.g., immune cells, e.g., B cells or T cells) are encapsulated dually into drops. In
the drops, the cells are lysed and mRNA is captured onto beads, which contain oligonucleotides to
hybridize to mRNA. The beads tate maintaining native pairing information (e.g., the native
pairing n two chains, e.g., a heavy chain and a light chain in a single B cell; an 0t chain and a [3
chain in a single T cell; or a 7 chain and a 6 chain in a single T cell). Next, the mRNA is reversed
transcribed to cDNA by a reverse transcriptase (RT). The e transcription can be performed
within the lysis drops, outside of drops, or in the subsequent drop (‘PCR’ drop). Beads having
captured mRNA or cDNA are recovered from the initial drops. The beads are then ulated into
new drops, wherein the nucleic acids are amplified, either by RT-PCR (when mRNA is template) or
PCR (when cDNA is template). The cDNAs encoding the two chains are amplified in drops. The
ied products are captured back onto beads by specific complementary nucleic acid
hybridization. The beads having captured products are recovered from drops and subsequently
encapsulated into new drops. The amplified product encoding one chain (e.g., VH) is linked with the
amplification product encoding the other chain (e. g., VL) in drops using a thermostable ligase. In an
approach (“linking cohesive products”), cohesive (or “sticky-end”) PCR products are generated, and
covalent ligation of hybridized cohesive PCR products are performed by a thermostable ligase. In
another ch (“ligase cycling reaction”), no cohesive PCR products are produced. Rather, in
drops, DNA is linked together through use of a stable ligase and a splint (or bridging)
oligonucleotide. While not wishing to be bound by theory, it is believed that in an embodiment, the
methods described herein reduce or preclude the possibility of unintended fusing caused by overlap
extension PCR methods (Turchaninova et al. Eur J Immunol. 2013; 43(9): 2507-2515). The ligated
ts, representing natively paired chains, are further amplified to generate sufficient al to
create a display library, such as in yeast or phage. The amplified product, encoding natively paired
chains (e.g., antibody heavy chain and light chain, TCR 0L chain and [3 chain, or TCR 7 chain and 5
chain) in a format such as an scFv, scFab, Fab, or full-length IgG, are introduced to an appropriate
expression or display vehicle, such as yeast or phage display. The ucted y, e. g., having
>104 and up to 109 or larger members, can be rapidly interrogated for desired binding and/or other
phenotypic properties, using established methods.
Generation of Cohesive PCR Products That Are Suitable Substratesfor Ligase
In an embodiment, amplification (e.g., PCR) products with cohesive ends that are suitable
substrates for ligase are generated. Without wishing to be bound by theory, it is believed that in an
embodiment, DNA polymerase extension can be prematurely terminated at a defined location, e.g.,
through use of a chemically d (e.g., lesioned) nucleotide or base, or other alterations to the
primer used for amplification. These chemically modified nucleotides or bases (or other primer
alterations) are subsequently incorporated into one strand of the amplification t. As the DNA
polymerase reads h the template strand which contains the d nucleotide, it prematurely
stops extension at (or near) the modified nucleotide, as it is not able to read through. This early
polymerase termination due to the modification can lead to production of an ication product
with a cohesive end. The amplification product can hybridize (or anneal) ntly with another
ication product having a complementary ve end, which can be produced in an analogous
matter. For example, a PCR product encoding one chain (6.3., VH) and a PCR product encoding
another chain (e.g., VL), each having a complementary cohesive end, can hybridize (or anneal) to
each other with high efficiency. Next, a thermostable ligase, t in the droplet with the DNA
polymerase (e. g., throughout thermocycling), catalyzes ligation ent linkage) of the hybridized
(or annealed) DNA molecules.
In an embodiment, the native paring ation is maintained during amplification and
ligation. In an embodiment, both amplification and ligation occur in the same drop, e.g., without
breaking the drop. In an embodiment, the ligase retains at least 50%, e.g., at least 60%, 70%, 80%,
85%, 90%, 95%, or 99% activity, at 95°C or more (e.g., 96°C or more, 97°C or more, or 98°C or
more), during one or more (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, or more) thermocycles. In an
embodiment, the ligase retains at least 50%, e.g., at least 60%, 70%, 80%, 85%, 90%, 95%, or 99%
activity, at 95°C or more (e.g., 96°C or more, 97°C or more, or 98°C or more), for at least 5 s
(e. g., at least 10 minutes, 15 minutes, 20 minutes, 25 s, 30 s, 45 minutes, or 60 minutes).
In an ment, the ligase retains at least 50%, e.g., at least 60%, 70%, 80%, 85%, 90%, 95%, or
99% activity, at 95°C or more (e.g., 96°C or more, 97°C or more, or 98°C or more), in a buffer
ion that allows for DNA polymerase activity.
In an embodiment, the modification inhibits or blocks DNA polymerase activity and remains
a substrate for ligation. In an embodiment, the modification does not inhibit or prevent g of the
ication product to a ligase. In an embodiment, the modification does not inhibit or prevent
formation of a phosphodiester bond. In an embodiment, the modification does not comprise a large
bulky chemical group. Exemplary modifications include, but are not limited to, a ribose 2’-C (2nd
carbon) modification (e.g., OH (516., a ribonucleotide, not a deoxyribonucleotide), O-methyl (O-CH3),
or amine (NH2)); a ribose 4’-C (4th carbon) modification; a base modification (e.g., a non-native base,
a uracil, or others); an abasic site (e.g., an AP site or apyrimidine/apurine); or a staggered primer (e.g.,
different length ng). In an embodiment, the modification comprises a uracil and a DNA
polymerase that is inhibited by uracil (e.g., an archaeal DNA polymerase) is used for amplification.
Exemplary steps for performing a cohesive-end PCR-ligation ment in drops are
illustrated below.
Cell Encapsulation
Cells can be encapsulated individually into droplets. In an embodiment, the cell is an immune
cell. In an embodiment, the cell is a B cell. In an embodiment, the cell is a T cell. In an embodiment,
the cell is an antibody-producing cell. In an embodiment, the cell is an isolated cell or purified cell.
In an embodiment, the cell is obtained from a subject, e.g., a human, mouse, rabbit, rat, goat, sheep, or
chicken.
In an embodiment, the volume of the droplet is from 10 pL to 100 nL, e.g., from 10 pL to 100
pL, from 10 pL to 1000 pL, from 10 pL to 10 nL, from 10 nL to 100 nL, from 1000 pL to 100 nL,
from 100 pL to 100 nL, from 100 pL to 10 nL, from 100 pL to 1000 pL, from 1000 pL to 10 nL, or
from 100 pL to 1000 pL. In an embodiment, the volume of the droplet is from 100 pL to 1000 pL.
In an embodiment, the droplet is a water-in-oil droplet. In an embodiment, the droplet is
present in a r (e.g., oil) phase, e.g., a carrier phase comprising 3MTM HFE-7500 with about 1%
fiuorosurfactant (RAN Biotechnologies).
The droplets can be formed, e. g., using a microfluidic chip (e.g., 2R 100 pm from te)
with the flow of fluid phase controlled by a syringe or pressure pump. In an embodiment, the aqueous
phase of the droplet comprises a buffer, a reagent that aids cell lysis, and a bead. In an embodiment,
the buffer ses Tris at pH 7.5. In an embodiment, the reagent that aids cell lysis comprises a
detergent. Exemplary detergents that can be used to aid cell lysis include, but are not limited to,
Tween-20, Triton X, IGEPAL, or sodium lauroyl sarcosinate (Sarkosyl). In an embodiment, the bead
is a magnetic bead. In an ment, the bead comprises, is coupled to, an oligonucleotide (e. g., a
primer), e. g., to anneal to an mRNA (e. g., an mRNA encoding a heavy chain or a light chain).
In an embodiment, the droplet contains no more than one cell after encapsulation. In an
embodiment, the droplet contains a ity of beads. In an embodiment, a plurality of beads are
obtained, and at least 80%, e. g., at least 85%, 90%, 95 %, 98%, 99%, or 100%, of the plurality
contains no more than one cell per droplet. In an embodiment, a plurality of beads are obtained, and
at least 80%, e. g., at least 85%, 90%, 95%, 98%, 99%, or 100%, of the plurality contains at least one
bead per droplet. Typically, the occupancy of drops is no more than one cell per droplet, and at least
one bead per droplet.
Cell Lysis
After encapsulation, the droplets can be ted to facilitate cell lysis. In an embodiment,
an emulsion (e. g., containing ce different solution phases) is heated, e. g., to reduce mRNA
secondary structures so that it can be more efficiently captured onto the bead and/or to improved lysis
efficiency in the presence of a detergent (e. g., Tween20). In an embodiment, the emulsion is
incubated at a temperature n 40°C and 80°C, e. g., between 40°C and 60°C, 50°C and 70°C, or
60°C and 80°C, e. g., at 40°C, 50°C, 60°C, 70°C, or 80°C. In an embodiment, the emulsion is
incubated for 5 to 60 minutes, e.g., 10 to 45 minutes, 15 to 30 minutes, 5 to 30 minutes, or 30 to 50
minutes. In an ment, the cell is lysed by heat. In an embodiment, the cell is lysed by an
enzyme. Typically, after the cell is lysed, mRNA is released and is captured on a bead by annealing
to the oligonucleotides on the bead.
Bead ry
Emulsions (e.g., containing coalesce different solution phases) can be broken using a drop
ilizing reagent, e.g., perfluorooctanol (PFO). In an embodiment, the bead-containing aqueous
phase is recovered. In an embodiment, the bead is a magnetic bead, and is isolated using magnet. In
an ment, the bead is washed and resuspended in a buffer (e. g., Tris, pH 7.5).
Reverse Transcription
Reverse transcription can be performed using standard methods. In an embodiment, the
reverse transcription is performed in a ulsion reaction. In an embodiment, the reverse
transcription is performed in an emulsion reaction. In an ment, the bead with captured mRNA
is resuspended in a buffer-enzyme mix (e.g., Superscript II RT) and incubated at 35°C to 45°C (e. g.,
at 40°C) for 10 to 60 minutes (e.g., 15 minutes) to facilitate reverse ription. In an ment,
the oligonucleotide coupled to the bead is used as a primer for the synthesis of the first strand cDNA.
In an embodiment, the bead is washed with a buffer (e.g., Tris, pH 7.5) after reverse transcription.
Bead Encapsulation
Beads can be ulated individually into droplets. In an embodiment, the volume of the
droplet is from 5 pL to 500 pL, e. g., from 5 pL to 400 pL, from 5 pL to 300 pL, from 5 pL to 200 pL,
from 5 pL to 100 pL, from 5 pL to 50 pL, from 5 pL to 25 pL, from 400 pL to 500 pL, from 300 pL to
500 pL, from 200 pL to 500 pL, from 100 pL to 500 pL, from 50 pL to 500 pL, from 25 pL to 500 pL,
from 10 pL to 500 pL, from 10 pL to 400 pL, from 25 pL to 300 pL, from 50 pL to 200 pL, or from
pL to 50 pL. In an embodiment, the volume of the droplet is from 10 pL to 50 pL.
In an embodiment, the droplet is a water-in-oil droplet. In an ment, the droplet is
present in a carrier (e.g., oil) phase, e.g., a carrier phase comprising 3MTM HFE-7500 with about 1%
urfactant (RAN Biotechnologies).
In an embodiment, the droplet contains one bead after encapsulation. In an embodiment, a
plurality of beads are obtained, and at least 80%, e.g., at least 85%, 90%, 95%, 98%, 99%, or 100%,
of the plurality contains no more than one bead per droplet.
PCR-Ligarion Reaction
In an ment, a PCR-ligation reaction is performed. In an embodiment, the PCR-
ligation reaction generates a ligated product (e.g., a -stranded DNA) that comprises a
nucleotide sequence that encodes an antibody heavy chain variable region (or a n thereof) and
an antibody light chain variable region (or a portion thereof). In an embodiment, the PCR-ligation
reaction generates a d product (e.g., a double-stranded DNA) that comprises a nucleotide
sequence that encodes a TCR (1 Chain (or a portion thereof) and a TCR [3 chain (or a portion thereof).
In an embodiment, the PCR-ligation reaction generates a ligated product (e.g., a double-stranded
DNA) that comprises a nucleotide sequence that encodes a TCR 7 chain (or a portion thereof) and a
TCR 5 chain (or a portion thereof). In an embodiment, the PCR-ligation reaction is performed in a
droplet comprising a bead that is d with cDNA, a DNA polymerase, oligonucleotides (e.g., for
amplification of the cDNA), a ligase (e.g., a thermostable ), and a .
Exemplary DNA polymerases that can be used in the reaction include, but are not d to,
n® High-Fidelity DNA Polymerase (NEB), Q5® High-Fidelity DNA Polymerase (NEB), Pfu
DNA polymerase, KAPA DNA polymerase, Vent® DNA polymerase, or Taq DNA polymerase.
In an ment, the ligated product contains an scFv, a Fab, or scFab cassette. In an
embodiment, the te (e.g., scFv cassette) is constructed as VL-Linker-VH. Without wishing to
be bound by theory, it is believed that in an embodiment, the order can be switched to VH-Linker-VL,
with no significant impact on expression or function. In an embodiment, the cassette (e.g., scFv)
cassette is constructed as VH-Linker-VL. In an embodiment, the cassette comprises a constant region
sequence (e.g., a CH1 domain and/or a CL domain), e.g., VH-CHl coupled with VL-CL.
Similarly, the ligated t can contain a cassette constructed as at chain-Linker-B chain or
[3 chain-Linker-a chain, or y chain-Linker-5 chain or 5 chain-Linker-y chain.
In an embodiment, the reverse primer for the VL sequence contains one, two, or all of the
following: (a) an ng sequence encoding a linker sequence (b) at least one modified nucleotide
(e.g., 3 consecutive nucleotides with 2’-O-methy1 modification), e.g., in the overhang; or (c) a 5’-
phosphate. In an embodiment, the forward primer for the VH sequence contains one, two, or all of
the following: (a) an ng sequence encoding a linker sequence (b) at least one modified
tide (e.g., 3 consecutive nucleotides with 2’-O-methyl modification), e.g., in the overhang; or
(c) a sphate.
In an embodiment, the reverse primer for the VH ce contains one, two, or all of the
following: (a) an overhang ce encoding a linker sequence (b) at least one modified nucleotide
(e. g., 3 consecutive nucleotides with 2’-O-methyl modification), e.g., in the overhang; or (c) a 5’-
phosphate. In an embodiment, the forward primer for the VL sequence contains one, two, or all of the
following: (a) an overhang sequence encoding a linker sequence (b) at least one modified nucleotide
(6.3., 3 consecutive nucleotides with 2’-O-methyl modification), e.g., in the overhang; or (c) a 5’-
phosphate.
Similarly, in an ment, the reverse primer for the OL chain (or 7 chain) sequence ns
one, two, or all of the following: (a) an overhang sequence encoding a linker ce (b) at least one
modified nucleotide (e.g., 3 consecutive nucleotides with 2’-O-methyl modification), e.g., in the
overhang; or (c) a 5’-phosphate. In an embodiment, the forward primer for the B chain (or 6 chain)
sequence contains one, two, or all of the ing: (a) an overhang ce ng a linker
sequence (b) at least one modified nucleotide (e. g., 3 consecutive nucleotides with 2’-O-methyl
modification), e.g., in the overhang; or (c) a sphate.
Similarly, in an embodiment, the e primer for the [3 chain (or 5 chain) sequence contains
one, two, or all of the following: (a) an overhang sequence encoding a linker sequence (b) at least one
modified nucleotide (e.g., 3 consecutive nucleotides with 2’-O-methyl modification), e.g., in the
overhang; or (c) a 5’-phosphate. In an embodiment, the forward primer for the 0: chain (or 7 chain)
sequence contains one, two, or all of the following: (a) an overhang sequence encoding a linker
sequence (b) at least one modified nucleotide (e.g., 3 consecutive tides with 2’-O-methyl
modification), e.g., in the ng; or (c) a 5’-phosphate.
Exemplary ligases (e.g., thermostable ligases) that can be used in the reaction include, but are
not limited to, Taq DNA ligase, Pfu DNA ligase, Ampligase® thermostable DNA ligase, Tsc DNA
ligase, Rma DNA ligase, Tfi DNA ligase, or Tth DNA ligase.
In an embodiment, the buffer supports both DNA polymerase and ligase enzymatic activities.
In an embodiment, the thermocycling is performed with emulsion (e.g., in a PCR tube). In an
embodiment, the thermocycling is performed using the following conditions: initial denaturation at
95-98°C for 30 seconds to 2 minutes; 10-30 cycles of: ration at 95-98°C for 10-30 seconds,
primer annealing at 50-60°C for 10-30 seconds, polymerase ion at 72°C for 30 seconds, and
cohesive product annealing and ligation at 45-55°C for 3 minutes. The reaction can be hold at 4°C.
Recovery ofAqueous Portion
Emulsions (e.g., containing coalesce different solution phases) can be broken using a drop
destabilizing reagent, e. g., perfluorooctanol (PFO). In an embodiment, the aqueous portion (e. g.,
containing linked product, and optionally, nked product) is recovered. In an embodiment, the
bead is discarded.
Purification ofLinked t
Linked product (e.g., enting natively linked VL—linker-VH) can be purified from non-
linked products (e. g., non-linked VH and VL). The ligated products are separated from non-ligated
products by size separation. For example, denaturing PAGE crylamide gel ophoresis) or
denaturing HPLC-SEC can be used. The linked product (~800-900 bp) is isolated from non-linked
product (~350-500 bp). For denaturing PAGE purification, the ligated band is cut out from the gel
and an elecro-elution is med to extract DNA from gel slice (Bio-Rad Electro-Elutor).
Amplification ofPurified Linked Product
The purified linked product can be amplified, e. g., by PCR. For example, the purified linked
product is amplified by PCR using a DNA polymerase (e.g., Taq polymerase) under conditions that
can moderately read through DNA containing modified nucleotides.
The final PCR product can be introduced to yeast using standard methods (e.g.,
electroporation with expression vector) to create a ly paired library d from biological
SOUICCS.
Ligase Cycling
In an embodiment, the different chains (e. g., VH and VL) are not amplified in a manner
which incorporates DNA sequence common to both chains, which would facilitate annealing of
sticky-end ts directly to each other. In an embodiment, a bridging (or ) oligonucleotide is
included after the amplification of cDNA but in the presence of a thermostable ligase. The bridging
oligonucleotide can facilitate bringing the two chains immediately adjacent to each other such that
they become a substrate of . Ligase, in turn, catalyzes a covalent bond formation between
chains of DNA. Since this mechanism does not lead to incorporation of sequence common to both
chains in each chain (e.g., overhang DNA with common sequence in both VH and VL), there is no
opportunity for splicing by p ion PCR.
The steps of this approach are generally the same as above except beginning at the emulsion
PCR step. PCR amplification of cDNA can be performed in drops. s can add overhang
sequences, but there is generally no common sequence to both chains (e.g., VL and VH) that is added
(unlike the above strategy). The bead in the drop, through its ated oligonucleotides, becomes
filled or saturated with dsDNA ts of the two chains (e.g., VH and VL), each with specific
overhang sequence. Drops are broken, and any PCR product not linked or annealed to beads is
washed away. Beads containing dsDNA of two chains (e.g., VH and VL) are ulated into new
drops in the presence of a thermostable ligase and a splint oligonucleotide. In this emulsion format,
cycling is performed, which facilitates formation of the 3-DNA piece complex. This complex
is a substrate for ligase, which catalyzes covalent bond formation, linking the two . In an
embodiment, thermocycling aids sion of all ‘top strand’ DNA to linked product, until one
substrate becomes limiting. In another embodiment, both s become ligated. For example, once
the ‘top strand’ is d, it can serve as the ‘splint’ for the opposing strand, which ligase will
recognize as a substrate. Without wishing to be bound by theory, it is believed that, this facet
specifically makes the reaction nt, that is, initial ligated product can serve as more templates
(splints) to te even more additional d product. Drops are , and the ligated products
are amplified by rd PCR means.
ary steps for performing a ligase cycling experiment are illustrated below.
Cell Encapsulation
Cells can be encapsulated individually into droplets. In an embodiment, the cell is an immune
cell. In an embodiment, the cell is a B cell. In an embodiment, the cell is a T cell. In an embodiment,
the cell is an antibody-producing cell. In an embodiment, the cell is an isolated cell or purified cell.
In an embodiment, the cell is obtained from a subject, e.g., a human, mouse, rabbit, rat, goat, sheep, or
chicken.
In an embodiment, the volume of the droplet is from 10 pL to 100 nL, e.g., from 10 pL to 100
pL, from 10 pL to 1000 pL, from 10 pL to 10 nL, from 10 nL to 100 nL, from 1000 pL to 100 nL,
from 100 pL to 100 nL, from 100 pL to 10 nL, from 100 pL to 1000 pL, from 1000 pL to 10 nL, or
from 100 pL to 1000 pL. In an embodiment, the volume of the droplet is from 100 pL to 1000 pL.
In an embodiment, the droplet is a water-in-oil droplet. In an embodiment, the droplet is
present in a carrier (e.g., oil) phase, e.g., a carrier phase comprising 3MTM HFE-7500 with about 1%
fluorosurfactant (RAN Biotechnologies).
The droplets can be formed, e.g., using a microfluidic chip (e.g., 2R 100 pm from Dolomite)
with the flow of fluid phase controlled by a syringe or pressure pump. In an embodiment, the aqueous
phase of the droplet comprises a buffer, a reagent that aids cell lysis, and a bead. In an embodiment,
the buffer comprises Tris at pH 7.5. In an embodiment, the reagent that aids cell lysis comprises a
detergent. Exemplary detergents that can be used to aid cell lysis include, but are not limited to,
Tween-20, Triton X, IGEPAL, or sodium lauroyl sarcosinate (Sarkosyl). In an embodiment, the bead
is a magnetic bead. In an embodiment, the bead ses, is d to, an ucleotide (e. g., a
primer), e. g., to anneal to an mRNA (e. g., an mRNA encoding a heavy chain or a light chain).
In an embodiment, the droplet contains no more than one cell after encapsulation. In an
embodiment, the droplet contains a plurality of beads. In an embodiment, a plurality of beads are
obtained, and at least 80%, e.g., at least 85%, 90%, 95 %, 98%, 99%, or 100%, of the ity
contains no more than one cell per droplet. In an embodiment, a ity of beads are obtained, and
at least 80%, e.g., at least 85%, 90%, 95%, 98%, 99%, or 100%, of the plurality contains at least one
bead per droplet. Typically, the occupancy of drops is no more than one cell per droplet, and at least
one bead per droplet.
Cell Lysis
After encapsulation, the droplets can be incubated to facilitate cell lysis. In an embodiment,
an emulsion (e. g., containing coalesce different solution phases) is heated to improved lysis efficiency
in the presence of a detergent (e.g., Tween20). In an embodiment, the emulsion is incubated at a
temperature between 40°C and 80°C, e. g., between 40°C and 60°C, 50°C and 70°C, or 60°C and
80°C, e. g., at 40°C, 50°C, 60°C, 70°C, or 80°C. In an embodiment, the emulsion is incubated for 5 to
60 minutes, e.g., 10 to 45 minutes, 15 to 30 minutes, 5 to 30 minutes, or 30 to 50 minutes. In an
embodiment, the cell is lysed by heat. In an embodiment, the cell is lysed by an enzyme. Typically,
after the cell is lysed, mRNA is released and is captured on a bead by annealing to the
oligonucleotides on the bead.
Bead Recovery
Emulsions (e. g., containing coalesce different solution ) can be broken using a drop
destabilizing reagent, e.g., perfluorooctanol (PFO). In an embodiment, the bead-containing aqueous
phase is recovered. In an embodiment, the bead is a magnetic bead, and is isolated using magnet. In
an ment, the bead is washed and resuspended in a buffer (e. g., Tris, pH 7.5). In an
ment, the bead is kept cold to reduce dissociation of mRNA from the bead.
Reverse Transcription
Reverse transcription can be performed using rd s. In an embodiment, the
reverse ription is performed in a non-emulsion reaction. In an embodiment, the reverse
transcription is performed in an emulsion reaction. In a typical ment, the reverse transcription
step is performed within the PCR drop. For example, mRNA-beads are encapsulated into drops with
both reverse transcriptase and DNA polymerase to facilitate cDNA formation and dsDNA
amplification. In an embodiment, the bead with ed mRNA is resuspended in a buffer-enzyme
mix (e. g., Superscript II RT) and incubated at 35°C to 45°C (e. g., at 40°C) for 10 to 60 minutes (e.g.,
minutes) to facilitate e transcription. In an embodiment, the oligonucleotide coupled to the
bead is used as a primer for the synthesis of the first strand cDNA. In an embodiment, the bead is
washed with a buffer (e.g., Tris, pH 7.5) after reverse transcription.
Bead Encapsulationfor PCR
Beads can be encapsulated individually into droplets. In an embodiment, the volume of the
droplet is from 5 pL to 500 pL, e. g., from 5 pL to 400 pL, from 5 pL to 300 pL, from 5 pL to 200 pL,
from 5 pL to 100 pL, from 5 pL to 50 pL, from 5 pL to 25 pL, from 400 pL to 500 pL, from 300 pL to
500 pL, from 200 pL to 500 pL, from 100 pL to 500 pL, from 50 pL to 500 pL, from 25 pL to 500 pL,
from 10 pL to 500 pL, from 10 pL to 400 pL, from 25 pL to 300 pL, from 50 pL to 200 pL, or from
10 pL to 50 pL. In an embodiment, the volume of the droplet is from 10 pL to 50 pL.
In an embodiment, the t is a water-in-oil droplet. In an embodiment, the droplet is
present in a carrier (e.g, oil) phase, e.g., a carrier phase comprising 3MTM HFE-7500 with about 1%
fluorosurfactant (RAN Biotechnologies).
In an embodiment, the droplet contains one bead after ulation. In an embodiment, a
plurality of beads are obtained, and at least 80%, e.g., at least 85%, 90%, 95%, 98%, 99%, or 100%,
of the ity contains no more than one bead per droplet.
PCR Reaction
In an embodiment, a PCR reaction is performed. In an embodiment, the PCR reaction is
performed in a t comprising a bead that is coupled with cDNA, a DNA polymerase,
oligonucleotides (e.g., for amplification of the cDNA), and a buffer.
Exemplary DNA polymerases that can be used in the reaction include, but are not limited to,
Phusion® High-Fidelity DNA Polymerase (NEB), Q5® idelity DNA Polymerase (NEB), Pfu
DNA polymerase, KAPA DNA polymerase, Vent® DNA polymerase, or Taq DNA polymerase.
In an embodiment, the PCR product ns an scFv cassette. In an embodiment, the scFv
cassette is ucted as ker-VH. Without wishing to be bound by theory, it is believed that
in an embodiment, the order can be switched to ker-VL, with no cant impact on
expression or function. In an embodiment, the scFv cassette is constructed as VH-Linker-VL.
Similarly, the PCR product can contain a cassette constructed as or chain-Linker-B chain or [3
chain-Linker-a chain, or y chain-Linker-8 chain or 6 chain-Linker-y chain.
In an embodiment, a primer for a target le region sequence described herein can contain
(e.g., from 5’ to 3’): a first sequence that is complementary to the sequence of an oligonucleotide
attached to a capture substrate, a spacer (e.g., a spacer described herein, e.g., a PEG spacer), a
ce that is complementary to at least a portion of the first sequence, a universal priming
sequence, and a sequence complementary to the target variable region sequence.
In an embodiment, the reverse primer for the VL sequence contains one, two, or all of the
following: (a) an overhang ce encoding a linker sequence (b) at least one modified nucleotide
(e. 3., 3 consecutive nucleotides with 2’-O-methyl modification), e. g., in the ng; or (c) a 5’-
phosphate. In an embodiment, the forward primer for the VH sequence contains one, two, or all of
the following: (a) an overhang ce ng a linker sequence (b) at least one modified
nucleotide (e.g., 3 consecutive nucleotides with 2’-O-methyl modification), e. g., in the overhang; or
(c) a 5’-phosphate.
In an embodiment, the e primer for the VH sequence contains one, two, or all of the
following: (a) an overhang ce encoding a linker sequence (b) at least one modified nucleotide
(e. g., 3 consecutive nucleotides with 2’-O-methyl modification), e. g., in the overhang; or (c) a 5’-
phosphate. In an embodiment, the forward primer for the VL sequence contains one, two, or all of the
following: (a) an overhang sequence encoding a linker sequence (b) at least one modified nucleotide
(e.g., 3 consecutive nucleotides with 2’-O-methyl modification), e. g., in the overhang; or (c) a 5’-
phosphate.
Similarly, in an embodiment, the reverse primer for the Qt chain (or 7 chain) sequence contains
one, two, or all of the ing: (a) an overhang sequence ng a linker ce (b) at least one
modified nucleotide (e.g., 3 consecutive nucleotides with 2’-O-methyl modification), e. g., in the
ng; or (c) a 5’-phosphate. In an embodiment, the forward primer for the B chain (or 5 chain)
sequence contains one, two, or all of the following: (a) an overhang sequence encoding a linker
sequence (b) at least one modified tide (e. g., 3 consecutive nucleotides with 2’-O-methyl
modification), e. g., in the overhang; or (c) a 5’-phosphate.
Similarly, in an embodiment, the reverse primer for the B chain (or 5 chain) sequence contains
one, two, or all of the following: (a) an overhang sequence encoding a linker sequence (b) at least one
modified nucleotide (e.g., 3 consecutive nucleotides with 2’-O-methyl modification), e. g., in the
overhang; or (c) a 5’-phosphate. In an ment, the forward primer for the a chain (or 7 chain)
sequence contains one, two, or all of the following: (a) an ng sequence encoding a linker
sequence (b) at least one modified nucleotide (e. g., 3 consecutive nucleotides with 2’-O-methyl
modification), e. g., in the overhang; or (c) a 5’-phosphate.
In an embodiment, the cycling is performed with emulsion (e. g., in a PCR tube). In an
embodiment, the thermocycling is med using the ing conditions: initial denaturation at
95-98°C for 30 seconds to 2 s; 10-30 cycles of: denaturation at 95-98°C for 10-30 seconds,
primer annealing at 50-60°C for 10-30 seconds, and polymerase ion at 72°C for 30 seconds. In
an embodiment, the reaction undergoes a slow cooling to facilitate capture of PCR products onto
beads. The reaction can be hold at 4°C.
Bead Recovery
Emulsions (e.g., containing coalesce different solution phases) can be broken using a drop
destabilizing reagent, e. g., perfluorooctanol (PFO). In an embodiment, the bead-containing aqueous
phase is recovered. In an embodiment, the bead is a magnetic bead, and is isolated using magnet. In
an embodiment, the bead is washed and resuspended in a buffer (e. g., Tris, pH 7.5).
Bead Encapsulationfor Ligase Cycling
Beads can be encapsulated individually into droplets. In an embodiment, the volume of the
droplet is from 5 pL to 500 pL, e. g., from 5 pL to 400 pL, from 5 pL to 300 pL, from 5 pL to 200 pL,
from 5 pL to 100 pL, from 5 pL to 50 pL, from 5 pL to 25 pL, from 400 pL to 500 pL, from 300 pL to
500 pL, from 200 pL to 500 pL, from 100 pL to 500 pL, from 50 pL to 500 pL, from 25 pL to 500 pL,
from 10 pL to 500 pL, from 10 pL to 400 pL, from 25 pL to 300 pL, from 50 pL to 200 pL, or from
10 pL to 50 pL. In an embodiment, the volume of the droplet is from 10 pL to 50 pL.
In an ment, the droplet is a water-in-oil droplet. In an embodiment, the droplet is
t in a carrier (e.g., oil) phase, e. g., a carrier phase comprising 3MTM HFE-7500 with about 1%
fluorosurfactant (RAN Biotechnologies).
In an embodiment, the droplet contains one bead after encapsulation. In an embodiment, a
plurality of beads are obtained, and at least 80%, e.g., at least 85%, 90%, 95%, 98%, 99%, or 100%,
of the ity contains no more than one bead per droplet.
Ligase Cycling Reaction
In an embodiment, a ligase cycling reaction is med. In an ment, the ligase
cycling reaction is performed in a droplet comprising a bead that is coupled with PCR product, a
Splint oligonucleotide (e. g., complementary and anneals to 3’ terminus of one strand (e. g., “top” VL
strand) and 5’ us of another strand (e. g., “top” VH strand)), a thermostable ligase, and one or
more reaction components that supports ligase enzymatic ty (e. g., NAD).
Exemplary ligases (e. g., thermostable ligases) that can be used in the reaction include, but are
not limited to, Taq DNA ligase, Pfu DNA ligase, Ampligase® thermostable DNA ligase, Tsc DNA
ligase, Rma DNA ligase, Tfi DNA ligase, or Tth DNA ligase.
In an embodiment, the thermocycling is performed with emulsion (e. g., in a PCR tube). In an
embodiment, the thermocycling is performed using the following conditions: 3- 15 cycles of:
ration at 90-95°C for 30 seconds, and annealing and ligation at 50-60°C for 1-3 minutes. The
on can be hold at 4°C.
Recovery ous Portion
Emulsions (e.g., containing coalesce different solution phases) can be broken using a drop
destabilizing reagent, e. g., perfluorooctanol (PFO). In an embodiment, the aqueous n (e. g.,
containing linked product, and ally, non-linked product) is recovered. In an embodiment, the
bead is discarded.
Purification ofLinked Product
Linked product (e.g., representing natively linked VL—linker-VH) can be purified from non-
linked products (e.g., non-linked VH and VL). The ligated products are separated from gated
products by size separation. For example, denaturing PAGE (polyacrylamide gel electrophoresis),
denaturing EC, agarose gel electrophoresis or AMPure XP beads can be used. The linked
product (~800-900 bp) is isolated from non-linked product (~350-500 bp). For denaturing PAGE
purification, the ligated band is cut out from the gel and an -elution is performed to extract
DNA from gel slice (Bio-Rad Electro-Elutor).
Amplification ofPurified Linked Product
The purified linked t can be amplified, e.g., by PCR. For example, the purified linked
product is amplified by PCR using a DNA polymerase (e.g., Taq polymerase) under standard
conditions with oligonucleotides that anneal the outer termini of the ligated product.
The final PCR product can be introduced to yeast or mammalian cells using standard methods
(e.g., electroporation with expression vector) to create a natively paired library derived from
ical sources.
Exemplary steps in a method of making a nucleic acid comprising a sequence that encodes a
heavy chain element (HC element) of an antibody heavy chain variable region (HCVR) and a light
chain element (LC element) of an antibody light chain variable region (LCVR), and n the
HCVR and LCVR are matched, are illustrated in FIGS. 2A-2D.
Additional Exemplary Methods
In an aspect, the sure features a method of making a nucleic acid ce comprising a
sequence that encodes a heavy chain element (HC element) of an antibody heavy chain variable
region (HCVR) and a light chain element (LC t) of an antibody light chain variable region
(LCVR), and wherein the HCVR and LCVR are matched, the method sing carrying out the
following steps from A1, B1, C1, and D1, thereby making a c acid sequence comprising
a sequence that encodes an HC element of an HCVR and a LC element of an LCVR, wherein the
HCVR and LCVR are matched.
In an aspect, the disclosure es a method of making a nucleic acid sequence comprising a
sequence that encodes a heavy chain element (HC element) of an antibody heavy chain variable
region (HCVR) and a light chain element (LC element) of an antibody light chain le region
(LCVR), and wherein the HCVR and LCVR are matched, the method comprising ng out the
following steps from A1, B1, C1, D2, and E1, thereby making a nucleic acid sequence
comprising a sequence that encodes an HC element of an HCVR and a LC element of an LCVR,
wherein the HCVR and LCVR are matched.
In an aspect, the disclosure features a method of making a c acid sequence comprising a
sequence that encodes a heavy chain element (HC element) of an antibody heavy chain variable
region (HCVR) and a light chain element (LC element) of an dy light chain variable region
(LCVR), and wherein the HCVR and LCVR are matched, the method comprising ng out the
following steps from A1, B1, C2, and D3, thereby making a nucleic acid ce comprising
a ce that encodes an HC element of an HCVR and a LC element of an LCVR, n the
HCVR and LCVR are matched.
In an aspect, the disclosure features a method of making a nucleic acid ce comprising a
sequence that encodes a heavy chain element (HC element) of an antibody heavy chain le
region (HCVR) and a light chain t (LC element) of an antibody light chain variable region
(LCVR), and wherein the HCVR and LCVR are matched, the method comprising carrying out the
following steps from A1, B1, C2, D4 and E2, thereby making a nucleic acid sequence
comprising a ce that encodes an HC element of an HCVR and a LC element of an LCVR,
wherein the HCVR and LCVR are matched.
In an aspect, the disclosure features a method of making a nucleic acid ce comprising a
sequence that encodes a heavy chain element (HC element) of an dy heavy chain variable
region (HCVR) and a light chain element (LC element) of an antibody light chain variable region
(LCVR), and wherein the HCVR and LCVR are matched, the method comprising carrying out the
following steps from A1, B1, C3, and D5, y making a nucleic acid sequence comprising
a sequence that encodes an HC element of an HCVR and a LC element of an LCVR, wherein the
HCVR and LCVR are matched.
In an aspect, the disclosure features a method of making a nucleic acid sequence comprising a
sequence that encodes a heavy chain element (HC element) of an antibody heavy chain variable
region (HCVR) and a light chain element (LC element) of an antibody light chain variable region
(LCVR), and wherein the HCVR and LCVR are matched, the method comprising carrying out the
following steps from A1, B1, C3, D6 and E3, thereby making a nucleic acid sequence
sing a sequence that encodes an HC element of an HCVR and a LC element of an LCVR,
wherein the HCVR and LCVR are matched.
In the aforesaid exemplary s, the cDNA is typically not captured on the substrate (e.g.,
bead) in this workflow concept. For example, mRNA dissociates from the substrate (e.g., bead), then
cDNA is made in solution in the isolated reaction site (e.g., micro-chamber), e.g., in the drop, and
then PCR product is made from cDNA as template in solution in the isolated reaction site (e.g., micro-
chamber), e. g., in drop. In an embodiment, the method es an RT-PCR reaction, where both
enzymatic steps occur in solution in drop. In the aforesaid exemplary methods, the amplified products
are typically captured onto the substrate (e.g., bead), which can facilitate transitioning the paired
products into the next isolated reaction site (e.g., micro-chamber), e.g., the next drop.
Antibody Molecules
Disclosed herein are antibody molecules and libraries of antibody molecules. In an
embodiment, the antibody molecule or library of antibody molecules are made by a method described
herein.
As used herein, the term “antibody molecule” refers to a protein, e.g., an immunoglobulin
chain or a fragment thereof, sing at least one imrnunoglobulin variable domain sequence. The
term “antibody molecule” includes, for example, full-length, mature antibodies and antigen-binding
fragments of an antibody. For example, an antibody molecule can include a heavy (H) chain variable
domain sequence (abbreviated herein as VH), and a light (L) chain variable domain sequence
(abbreviated herein as VL). In another example, an dy molecule includes two heavy (H) chain
variable domain sequences and two light (L) chain variable domain sequence, y forming two
antigen binding sites, such as Fab, Fab’, F(ab’)2, Fc, Fd, Fd’, Fv, single chain antibodies (scFv for
e), single variable domain antibodies, diabodies (Dab) (bivalent and ific), and chimeric
(e.g., humanized) antibodies, which may be produced by the modification of whole antibodies or
those synthesized de novo using recombinant DNA technologies. These functional antibody
fragments retain the ability to selectively bind with their tive antigen or receptor. Antibodies
and antibody fragments can be from any class of antibodies including, but not limited to, IgG, IgA,
IgM, IgD, and IgE, and from any subclass (e.g., IgGl, IgGZ, IgG3, and IgG4) of antibodies. The
dy molecules can be monoclonal or polyclonal. The antibody molecule can also be a human,
zed, CDR-grafted, or in vitro generated antibody. The dy molecule can have a heavy
chain constant region chosen from, e.g., IgGl, IgGZ, IgG3, or IgG4. The antibody le can also
have a light chain chosen from, e.g., kappa or lambda. The term “immunoglobulin” (lg) is used
interchangeably with the term “antibody” herein.
Examples of antigen-binding fragments include: (i) a Fab fragment, a monovalent nt
consisting of the VL, VH, CL and CH1 domains; (ii) a F(ab')2 fragment, a bivalent fragment
comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd nt
consisting of the VH and CH1 domains; (iv) a Fv nt consisting of the VL and VH domains of a
single arm of an antibody, (v) a diabody (dAb) fragment, which consists of a VH domain; (vi) a
d or camelized variable domain; (vii) a single chain Fv (scFv), see e.g., Bird er al. (1988)
Science 242:423-426; and Huston er al. (1988) Proc. Natl. Acad. Sci. USA 9-5883); (viii) a
single domain antibody. These antibody fragments may be obtained using any suitable method,
including several tional techniques known to those with skill in the art, and the fragments can
be screened for utility in the same manner as are intact antibodies.
The term “antibody” includes intact les as well as functional fragments thereof.
Constant regions of the antibodies can be altered, e.g., mutated, to modify the properties of the
antibody (e.3., to se or decrease one or more of: Fc receptor binding, antibody glycosylation,
the number of ne residues, effector cell function, or complement function).
The antibody molecule can be a single chain antibody. A single-chain antibody (scFV) may
be engineered (see, for example, Colcher, D. et al. (1999) Ann N Y Acad Sci 880:263-80; and Reiter,
Y. (1996) Clin Cancer Res 2:245-52). The single chain antibody can be dimerized or multimerized to
generate multivalent dies having specificities for different epitopes of the same target protein.
The antibody les disclosed herein can also be single domain antibodies. Single
domain antibodies can e antibodies whose complementary determining regions are part of a
single domain polypeptide. es include, but are not limited to, heavy chain antibodies,
dies lly devoid of light chains, single domain antibodies derived from conventional 4-
chain antibodies, engineered dies and single domain scaffolds other than those derived from
antibodies. Single domain antibodies may be any of the art, or any future single domain antibodies.
Single domain antibodies may be derived from any s including, but not limited to mouse,
human, camel, llama, fish, shark, goat, , and bovine. According to some aspects, a single
domain antibody is a naturally occurring single domain antibody known as heavy chain antibody
devoid of light chains. Such single domain antibodies are disclosed in WO 94/04678, for example.
For clarity reasons, this variable domain derived from a heavy chain antibody naturally devoid of light
chain is known herein as a VHH or nanobody to distinguish it from the tional VH of four chain
immunoglobulins. Such a VHH molecule can be derived from antibodies raised in Camelidae
species, for example in camel, llama, dromedary, alpaca and guanaco. Other species besides
Camelidae may produce heavy chain antibodies naturally devoid of light chain; such VHHs are also
contemplated.
The VH and VL regions can be subdivided into regions of hypervariability, termed
“complementarity determining regions” (CDR), interspersed with regions that are more conserved,
termed “framework s” (FR or FW). The terms “complementarity determining region,” and
“CDR,” as used herein refer to the sequences of amino acids within antibody variable s which
confer n specificity and binding affinity. As used herein, the terms “framework,” “FW” and
“FR” are used interchangeably.
The extent of the framework region and CDRs has been precisely defined by a number of
methods (see, Kabat, E. A., et al. (1991) Sequences of Proteins of Immunological Interest, Fifth
Edition, US. Department of Health and Human Services, NIH Publication No. 91-3242; Chothia, C.
et al. (1987) J. Mol. Biol. 196:901-917; and the AbM definition used by Oxford Molecular’s AbM
antibody modeling software. See, generally, e.g., Protein Sequence and ure Analysis of
Antibody Variable Domains. In: Antibody Engineering Lab Manual (Ed.: Duebel, S. and
Kontermann, R., Springer-Verlag, Heidelberg). In an ment, the following definitions are used:
AbM definition of CDRl of the heavy chain variable domain and Kabat definitions for the other
CDRs. In an embodiment, Kabat definitions are used for all CDRs. In addition, embodiments
described with respect to Kabat or AbM CDRs may also be implemented using Chothia hypervariable
loops. Each VH and VL typically es three CDRs and four FRs, arranged from amino-terminus
to y-terminus in the following order: FRl, CDRl, FR2, CDRZ, FR3, CDR3, and FR4.
As used herein, an “immunoglobulin variable domain sequence” refers to an amino acid
sequence which can form the structure of an immunoglobulin le domain. For example, the
sequence may include all or part of the amino acid sequence of a naturally-occurring variable domain.
For example, the sequence may or may not e one, two, or more N- or C-terminal amino acids,
or may include other alterations that are compatible with formation of the protein structure.
The term “antigen-binding region” refers to the part of an antibody molecule that comprises
determinants that form an interface that binds to an antigen, or an epitope thereof. With respect to
proteins (or protein mimetics), the antigen-binding region lly includes one or more loops (of at
least, e.g., four amino acids or amino acid ) that form an interface that binds to the antigen.
Typically, the antigen-binding region of an antibody molecule includes at least one or two CDRs
andfor hypervariable loops, or more typically at least three, four, five or six CDRs and/or
hypervariable loops.
The terms “compete” or “cross-compete” are used interchangeably herein to refer to the
ability of an antibody molecule to interfere with binding of another antibody molecule, to a target.
The interference with binding can be direct or indirect (e. g., through an allosteric tion of the
antibody molecule or the target). The extent to which an antibody le is able to interfere with
the binding of another antibody molecule to the , and therefore whether it can be said to
compete, can be ined using a competition binding assay, for example, a FACS assay, an ELISA
or BIACORE assay. In an embodiment, a competition binding assay is a quantitative competition
assay. In an embodiment, a first antibody molecule is said to compete for g to the target with a
second antibody molecule when the binding of the first antibody molecule to the target is d by
% or more, e.g., 20% or more, 30% or more, 40% or more, 50% or more, 55% or more, 60% or
more, 65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 90% or more, 95% or
more, 98% or more, 99% or more in a competition binding assay (e.g., a competition assay described
herein).
The terms “monoclonal antibody” or “monoclonal antibody composition” as used herein refer
to a preparation of antibody molecules of single molecular composition. A monoclonal antibody
composition displays a single binding specificity and ty for a particular e. A monoclonal
antibody can be made by hybridoma technology or by methods that do not use hybridoma technology
(e.g., recombinant methods).
An “effectively human” protein is a protein that does not evoke a neutralizing antibody
response, e.g., the human urine antibody (HAMA) response. HAMA can be problematic in a
number of circumstances, e. g., if the antibody molecule is stered repeatedly, e. g., in treatment
of a chronic or recurrent disease condition. A HAMA response can make repeated antibody
administration potentially ineffective because of an increased antibody nce from the serum (see,
e.g., Saleh et al., Cancer Immunol. Immunother. 32:180-190 (1990)) and also because of potential
allergic reactions (see, e. g., LoBuglio et at, Hybridoma, 5:5117-5123 (1986)).
The antibody molecule can be a onal or a monoclonal antibody. In some embodiments,
the antibody can be inantly produced, e. g., produced by any suitable phage display or
combinatorial methods.
Various phage display and combinatorial methods for generating antibodies are known in the
art (as described in, e. g., Ladner et al. U.S. Patent No. 5,223,409; Kang et al. International Publication
No. W0 92/18619; Dower et al. International Publication No. WO 91/17271; Winter et al.
International Publication WO 92/20791; Markland et al. International Publication No. W0 92/ 15679;
Breitling et al. International Publication WO 93/01288; McCafferty et al. International Publication
No. WO 92/01047; Garrard et al. International Publication No. WO 90; Ladner et al.
International Publication No. WO 90/02809; Fuchs et al. (1991) Bio/Technology 9: 1370-1372; Hay et
al. (1992) Hum Antiboa’ Hybridomas 3:81-85; Huse et al. (1989) Science 246: 1275-1281; hs er
al. (1993) EMBO J 12:725-734; Hawkins et al. (1992) JMol Biol 226:889-896; Clackson et al. (1991)
Nature 352:624-628; Gram et al. (1992) PNAS 89:3576-3580; Garrad et al. (1991) chnology
9: 1373-1377; Hoogenboom et al. (1991) Nuc Acid Res 19:4133-4137; and Barbas et al. (1991) PNAS
88:7978-7982, the contents of all of which are incorporated by reference herein).
In an embodiment, the antibody molecule is a fully human antibody (e. g., an antibody made
in a mouse which has been genetically engineered to produce an antibody from a human
immunoglobulin sequence), or a man antibody, e. g., a rodent (mouse or rat), goat, e
(e. g., monkey), camel antibody. In an embodiment, the non-human antibody is a rodent (mouse or rat
antibody). Methods of producing rodent dies are known in the art.
Human monoclonal antibodies can be generated using transgenic mice carrying the human
immunoglobulin genes rather than the mouse system. cytes from these transgenic mice
immunized with the antigen of interest are used to produce hybridomas that secrete human mAbs with
specific affinities for epitopes from a human n (see e. g., Wood et al. ational Application
WO 06, Kucherlapati et al. PCT publication W0 91/ 10741; Lonberg et al. International
Application WO 92/03918; Kay et al. International Application 92/03917; Lonberg, N. et al. 1994
Nature 368:856-859; Green, L.L. et al. 1994 Nature Genet. 7:13-21; on, S.L. et al. 1994 Proc.
Natl. Acad. Sci. USA 1-6855; Bruggeman et al. 1993 Year Immunol 7:33-40; Tuaillon et al.
1993 PNAS 90:3720—3724; Bruggeman et al. 1991 Eur J l 21:1323-1326).
An antibody can be one in which the variable , or a portion thereof, e. g., the CDRs, are
generated in a man organism, e.g., a rat or mouse. Chimeric, CDR-grafted, and humanized
antibodies are within the invention. Antibodies generated in a non-human organism, e. g., a rat or
mouse, and then modified, e.g., in the variable framework or constant , to decrease antigenicity
in a human are within the invention.
Chimeric antibodies can be produced by any suitable recombinant DNA technique. Several
are known in the art (see Robinson et al., International Patent Publication PCT/US86/02269; Akira, et
al., European Patent Application 7; Taniguchi, M., European Patent Application 171,496;
Morrison et al., European Patent Application 4; Neuberger et al., International Application WO
86301533; Cabilly et al. US. Patent No. 4,816,567; Cabilly et al., European Patent Application
125,023; Better et al. (1988 Science 240: 1041-1043); Liu et al. (1987) PNAS 9-3443; Liu et
al., 1987, J. l. 139:3521-3526; Sun et al. (1987) PNAS 84:214-218; Nishimura et al., 1987,
Canc. Res. 47:999-1005; Wood et al. (1985) Nature 314:446—449; and Shaw et al., 1988, J. Natl
Cancer Inst. 80: 1553- 1559).
A humanized or CDR-grafted antibody will have at least one or two but generally all three
recipient CDRs (of heavy and or light globulin chains) replaced with a donor CDR. The
antibody may be replaced with at least a portion of a non-human CDR or only some of the CDRs may
be replaced with man CDRs. It is only ary to replace the number of CDRs required for
binding of the humanized antibody to an antigen. In an embodiment, the donor will be a rodent
antibody, e. g., a rat or mouse antibody, and the recipient will be a human framework or a human
consensus framework. Typically, the globulin providing the CDRs is called the “donor” and
the immunoglobulin providing the framework is called the tor.” In some embodiments, the
donor immunoglobulin is a non-human (e.g., rodent). The acceptor framework is typically a
naturally-occurring (e.g., a human) framework or a consensus framework, or a sequence about 85% or
higher, e.g., 90%, 95%, 99% or higher identical thereto.
As used herein, the term nsus sequence” refers to the sequence formed from the most
frequently occurring amino acids (or tides) in a family of related sequences (See e.g., Winnaker,
From Genes to Clones (Verlagsgesellschaft, Weinheim, Germany 1987). In a family of proteins, each
position in the sus sequence is occupied by the amino acid occurring most frequently at that
on in the family. If two amino acids occur y frequently, either can be included in the
consensus sequence. A “consensus framework” refers to the framework region in the consensus
immunoglobulin sequence.
An antibody can be humanized by any suitable method, and several such s known in
the art (see e.g., Morrison, S. L., 1985, Science 229: 1202-1207, by Oi et al., 1986, BioTechniqaes
4:214, and by Queen et al. US 5,585,089, US 5,693,761 and US 5,693,762, the contents of all of
which are hereby incorporated by reference).
Humanized or CDR-grafted antibodies can be produced by CDR-grafting or CDR
substitution, wherein one, two, or all CDRs of an immunoglobulin chain can be replaced. See e.g.,
US. Patent 5,225,539; Jones et al. 1986 Nature 321 :552-525; Verhoeyan et al. 1988 Science
239:1534; r et al. 1988 J. Immunol. 141:4053-4060; Winter US 5,225,539, the contents of all of
which are hereby expressly incorporated by reference. Winter describes a CDR-grafting method
which may be used to prepare humanized antibodies (UK Patent Application GB 2188638A, filed on
March 26, 1987; Winter US 5,225,539), the contents of which is expressly incorporated by nce.
Also provided are humanized antibodies in which specific amino acids have been tuted,
deleted or added. Criteria for selecting amino acids from the donor are bed in, e. g., US
,585,089, e.g., s 12-16 of US 5,585,089, the contents of which are hereby incorporated by
nce. Other techniques for humanizing antibodies are described in Padlan et al. EP 519596 A1,
published on December 23, 1992.
In an embodiment, the dy le has a heavy chain constant region chosen from,
e.g., the heavy chain constant regions of IgG1, IgG2 (e.g., IgG2a), IgG3, IgG4, IgM, IgAl, IgA2,
IgD, and IgE; particularly, chosen from, e.g., the (e.g., human) heavy chain constant regions of IgG1,
IgG2, IgG3, and IgG4. In another embodiment, the antibody molecule has a light chain constant
region chosen from, e. g., the (e.g., human) light chain constant regions of kappa or lambda. The
constant region can be altered, e. g., mutated, to modify the properties of the dy molecule (e.g.,
to increase or decrease one or more of: Fc receptor g, antibody glycosylation, the number of
cysteine residues, effector cell function, and/or complement on). In an embodiment, the
dy molecule has or function and can fix complement. In another embodiment, the
antibody molecule does not recruit effector cells or fix complement. In certain embodiments, the
antibody molecule has reduced or no ability to bind an Fc receptor. For example, it may be an isotype
or subtype, fragment or other mutant, which does not support binding to an Fc or, e. g., it has a
mutagenized or deleted Fc receptor binding region.
In an embodiment, a constant region of the antibody molecule is altered. Methods for altering
an antibody constant region are known in the art. dy molecules with altered function, e.g.
altered affinity for an effector ligand, such as FcR on a cell, or the C1 component of complement can
be produced by replacing at least one amino acid residue in the constant portion of the antibody with a
different residue (see e.g., EP 388,151 A1, US. Pat. No. 5,624,821 and US. Pat. No. 5,648,260, the
contents of all of which are hereby incorporated by reference). Amino acid mutations which stabilize
antibody structure, such as S228P (EU nomenclature, S241P in Kabat nomenclature) in human IgG4
are also contemplated. Similar type of alterations could be described which if applied to the murine,
or other species immunoglobulin would reduce or ate these functions.
In an embodiment, the only amino acids in the antibody molecule are canonical amino acids.
In an embodiment, the antibody le comprises naturally-occurring amino acids; analogs,
derivatives and congeners thereof; amino acid analogs having variant side chains; and/or all
stereoisomers of any of any of the foregoing. The antibody molecule may comprise the D- or L-
optical s of amino acids and peptidomimetics.
A polypeptide of an antibody molecule described herein may be linear or branched, it may
comprise modified amino acids, and it may be interrupted by non-amino acids. The antibody
WO 19402
molecule may also be modified; for example, by disulfide bond ion, glycosylation, lipidation,
acetylation, phosphorylation, or any other manipulation, such as conjugation with a labeling
component. The polypeptide can be isolated from natural sources, can be a produced by recombinant
techniques from a otic or prokaryotic host, or can be a product of synthetic procedures.
The antibody molecule bed herein can be used alone in unconjugated form, or can be
bound to a substance, e. g., a toxin or moiety (e.g., a therapeutic drug; a compound emitting radiation;
molecules of plant, fungal, or bacterial origin; or a biological protein (e.g., a protein toxin) or particle
(e.g., a recombinant Viral particle, e.g., Via a Viral coat protein). For example, the antibody molecule
can be coupled to a radioactive isotope such as an (1-, [3-, or y-emitter, or a B-and y-emitter.
An antibody molecule can be derivatized or linked to another functional molecule (e.g.,
another peptide or protein). As used herein, a “derivatized” antibody molecule is one that has been
modified. Methods of derivatization include but are not limited to the addition of a cent
moiety, a radionucleotide, a toxin, an enzyme or an affinity ligand such as biotin. Accordingly, the
dy les are intended to include derivatized and otherwise modified forms of the
antibodies described herein, including immunoadhesion molecules. For example, an antibody
molecule can be functionally linked (by al coupling, genetic fusion, noncovalent association or
otherwise) to one or more other molecular entities, such as r antibody (e.g., a bispecific
antibody or a diabody), a detectable agent, a toxin, a pharmaceutical agent, andior a n or e
that can mediate association of the antibody or antibody portion with another molecule (such as a
avidin core region or a polyhistidine tag).
Some types of tized antibody molecule are produced by crosslinking two or more
antibodies (of the same type or of different types, e.g., to create bispecific antibodies). Suitable
crosslinkers include those that are heterobifunctional, having two distinctly reactive groups separated
by an appropriate spacer (e.g., m-maleimidobenzoyl-N-hydroxysuccinimide ester) or
homobifunctional (e.g., disuccinimidyl suberate). Such s are ble from Pierce Chemical
Company, Rockford, Ill.
Useful detectable agents with which an antibody molecule may be derivatized (or d) to
include cent compounds, various enzymes, prosthetic groups, luminescent materials,
bioluminescent materials, fluorescent emitting metal atoms, e.g., europium (Eu), and other anthanides,
and radioactive materials (described below). Exemplary cent detectable agents include
cein, fluorescein isothiocyanate, rhodamine, 5dimethylamine-l-napthalenesulfonyl chloride,
phycoerythrin and the like. An antibody may also be derivatized with detectable enzymes, such as
alkaline phosphatase, horseradish peroxidase, B-galactosidase, acetylcholinesterase, glucose oxidase
and the like. When an antibody is derivatized with a detectable enzyme, it is detected by adding
additional reagents that the enzyme uses to produce a detectable reaction product. For example, when
the detectable agent horseradish peroxidase is present, the addition of en peroxide and
diaminobenzidine leads to a colored reaction product, which is detectable. An antibody molecule may
also be tized with a etic group (e.g., streptavidin/biotin and avidin/biotin). For example,
an antibody may be derivatized with biotin, and detected through indirect measurement of avidin or
streptavidin binding. Examples of suitable fluorescent materials include umbelliferone, fluorescein,
fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or
phycoerythrin; an example of a luminescent material includes luminol; and examples of
bioluminescent materials include luciferase, luciferin, and aequorin.
Labeled antibody molecules can be used, for example, diagnostically and/or experimentally in
a number of contexts, ing (i) to isolate a predetermined antigen by standard techniques, such as
affinity tography or immunoprecipitation; (ii) to detect a predetermined antigen (e.g., in a
cellular lysate or cell supernatant) in order to evaluate the abundance and pattern of expression of the
protein; (iii) to monitor protein levels in tissue as part of a clinical testing procedure, e.g., to determine
the efficacy of a given treatment regimen.
An antibody molecule may be conjugated to another lar entity, typically a label or a
therapeutic (e.g., crobial (e. g., antibacterial or bactericidal), immunomodulatory,
immunostimularoty, cytotoxic, or cytostatic) agent or moiety. Radioactive isotopes can be used in
diagnostic or therapeutic applications. Radioactive es that can be coupled to the antibody
les include, but are not limited to or-, [3-, or y—emitters, or B-and y—emitters. Such radioactive
es include, but are not limited to iodine (1311 or 125I), yttrium (90Y), lutetium (177Lu), um
(225Ac), praseodymium, astatine (leAt), rhenium (186Re), bismuth (212Bi or 213Bi), indium (1 1 1In),
technetium (gngc), phosphorus (32F), rhodium (lgth), sulfur (35S) carbon (14C), tritium (3H),
chromium (5 lCr), chlorine (36Cl), cobalt (57Co or 58C0), iron (59Fe), selenium (75Se), or gallium (67Ga).
Radioisotopes useful as therapeutic agents include m (90Y), lutetium (177Lu), actinium (225Ac),
praseodymium, astatine (leAt), rhenium (186Re), bismuth (212Bi or 213Bi), and rhodium (lgth).
Radioisotopes useful as labels, e.g., for use in diagnostics, e iodine (1311 or 125I), indium 1),
technetium ), phosphorus (32F), carbon (14C), and tritium (3H), or one or more of the therapeutic
isotopes listed above.
The present disclosure provides radiolabeled antibody molecules and methods of labeling the
same. In an embodiment, a method of labeling an antibody molecule is disclosed. The method
includes ting an antibody molecule, with a ing agent, to thereby produce a conjugated
antibody. The conjugated antibody is radiolabeled with a sotope, e.g., 111Indium, 90Yttrium and
177Lutetium, to thereby produce a labeled antibody molecule.
In some aspects, this disclosure provides a method of making an antibody molecule disclosed
herein. The method includes: providing an antigen, or a fragment thereof; obtaining an antibody
molecule that specifically binds to the antigen; ting efficacy of the antibody molecule in
ting activity of the antigen and/or organism expressing the antigen. The method can further
e administering the antibody molecule, including a derivative thereof (6.3., a humanized
antibody molecule) to a subject, e.g., a human.
This disclosure provides an isolated nucleic acid molecule encoding the above antibody
molecule, vectors and host cells thereof. The nucleic acid molecule includes, but is not limited to,
RNA, genomic DNA and cDNA.
Other Binding Polypeptides
The disclosures herein are not intended to be limited to antibody molecules. The methods
described herein are broadly applicable to any binding polypeptides that have two or more chains
(e.g., having at least two paired or matched chains).
In an embodiment, the g molecule comprises an X chain variable region and a Y chain
variable region. For example, in any of the aspects, embodiments, and definitions herein, an dy
heavy chain (or variable region) can be replaced with an X chain (or variable ), and an antibody
light chain (or le region) can be replaced with a Y chain (or variable region).
In an aspect, the disclosure features a method of making a nucleic acid sequence comprising a
sequence that encodes an X chain element (XC element) of an antibody heavy chain variable region
(XCVR) and a Y chain t (YC element) of an antibody light chain variable region (YCVR), and
wherein the XCVR and YCVR are matched, the method comprising:
a) acquiring an isolated production reaction site, e.g., a production micro-chamber,
comprising:
i) an X chain (XC) strand, n the XC strand is a strand of an X chain -stranded
cDNA (XC ds cDNA) comprising a t that encodes an XC element of the XCVR from a cell,
e.g., an X chain variable region sequence (XCVRS); and
ii) a Y chain (YC) strand, wherein the YC strand is a strand of a Y chain double-stranded
cDNA (YC ds cDNA) comprising a segment that s a YC element of the YCVR from the cell,
e.g., a Y chain variable region sequence (YCVRS), and
b) nt linking, e.g., ligation, of an XC strand to a YC strand,
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding a YCVR or an XCVR from a cell other than the cell (e.g., a ent
cell),
thereby making a nucleic acid sequence comprising a sequence that encodes an XC element
of an XCVR and a YC element of a YCVR, wherein the XCVR and YCVR are matched.
“Matched,” as that term is used herein in connection with an X chain variable region and a Y
chain variable region, means they are from the same cell. In an embodiment, the X chain variable
region and the Y chain variable region can form a multimeric protein or a part of a multimeric protein.
With respect to an t of an X chain variable region and an element of a Y chain variable region
it means that the X chain variable region and the Y chain le region from which the ts are
derived are from the same cell.
An “X chain variable region sequence,” or “XCVRS,” as that term is used herein, refers to a
polypeptide comprising sufficient ce to allow binding of another polypeptide (e.g., an antigen).
In embodiments the XCVRS can assemble with a Y chain variable region, and, e.g., bind antigen. A
“Y chain variable region sequence,” or “YCVRS,” as that term is used herein, refers to a polypeptide
comprising ent sequence to allow binding of another polypeptide (e.g., an antigen). In
embodiments the YCVRS can assemble with an X chain variable region, and, e.g., bind antigen.
“Element” of an XC or YC le region, as that term is used herein, refers to a sequence
that encodes at least one amino acid. In an embodiment, an element comprises a CDR. In an
embodiment an element comprises a FW region.
In an ment, the XC element comprises, or consists of, an XCVRS. In an embodiment,
the YC element comprises, or consists of, a YCVRS.
In an embodiment, the XC ds cDNA comprises a segment that encodes an XCVRS. In an
embodiment, the YC ds cDNA ses a segment that encodes a YCVRS. In an embodiment, the
XC ds cDNA comprises a segment that encodes an XCVRS, and the YC ds cDNA comprises a
t that encodes a YCVRS.
In an embodiment, the cell is an immune cell, e.g., a B cell, e.g., a human B cell. In an
embodiment, the cell is a mammalian cell or an avian cell.
In an embodiment, the nucleic acid sequence is configured such that, when expressed, the XC
element and the YC element (e.g., the XCVRS and the YCVRS) form a functional n binding
molecule, e. g., a single chain or a complex of an XC and a YC. In an embodiment, the n
binding molecule is functional in vitro, ex vivo, or in vivo, e.g., as determined by a method or assay
described herein.
In an embodiment, acquiring an isolated production reaction site, e.g., a production micro-
chamber, comprises:
a) acquiring a capture substrate bound to: (i) a first double-stranded cDNA (ds cDNA)
comprising a strand that is complementary to a first mRNA that encodes an XCVR from a cell; and
(ii) a second ds cDNA sing a strand complementary to a second mRNA encoding a YCVR
from the cell (the cDNA loaded capture substrate), and
b) maintaining the ed production reaction site, e.g., the production micro-chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce: a plurality of
XC ds cDNAs comprising a segment that encodes an XC element of the XCVR from the cell, e.g., an
XCVRS; and a ity of YC ds cDNAs comprising a segment that encodes a YC element of the
YCVR from the cell, e.g., a YCVRS.
In an ment, the XC ds cDNA is identical, or substantially identical, to the first ds
cDNA. For example, the sense strand of the XC ds cDNA is at least 80%, 85%, 90%, 95%, 98%,
99%, or 100% identical to, or differs by no more than 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50
nucleotides from, the sense strand of the first ds cDNA, and/or the antisense strand of the XC ds
cDNA is at least 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to, or differs by no more than 1,
2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50 nucleotides from, the antisense strand of the first ds cDNA.
In an embodiment, the YC ds cDNA is cal, or substantially identical, to the second ds
cDNA. For example, the sense strand of the YC ds cDNA is at least 80%, 85 %, 90%, 95%, 98%,
99%, or 100% identical to, or differs by no more than 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50
nucleotides from, the sense strand of the second ds cDNA, and/or the antisense strand of the YC ds
cDNA is at least 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to, or differs by no more than 1,
2, 5, 10, 15, 20, 25, 30, 35, 40, 45 , or 50 nucleotides from, the antisense strand of the second ds
cDNA.
In an embodiment, the XC strand is a sense strand. In an embodiment, the YC strand is a
sense strand. In an embodiment, the XC strand is an antisense strand. In an embodiment, the YC
strand is an antisense . In an ment, both the XC strand and the YC strand are sense
strands. In an embodiment, both the XC strand and the YC strand are antisense strands.
In an embodiment, the capture substrate comprises a bead, e.g., a magnetic bead. In an
ment, the capture substrate comprises a moiety (e.g., an oligonucleotide) which binds to
cDNA, e.g., (i) a moiety which binds to the XC strand; (ii) a moiety which binds to the YC strand; or
(iii) both (i) and (ii). In an embodiment, the moiety which binds to the XC strand is ent from the
moiety which binds to the YC strand, e.g., to facilitate creating conditions favorable to capturing
similar levels of each DNA molecule type. In an embodiment, the moiety which binds to the XC
strand is identical to the moiety which binds to the YC strand.
In an embodiment, the first mRNA and the second mRNA are disposed on an mRNA loaded
capture substrate.
In an embodiment, the isolated production reaction site, e.g., the production chamber,
comprises: a reagent mixture suitable for producing, from the first and second mRNAs (e.g., after the
first and second mRNAs are released from the mRNA loaded capture substrate into a solution), a first
cDNA comprising a segment that s an XC t of the XCVR of the cell, e.g., an XCVRS,
and a second cDNA comprising a segment that encodes a YC element of the YCVR of the cell, e.g., a
YCVRS.
In an embodiment, the isolated production reaction site, e.g., production micro-chamber,
comprises primers that mediate the production of the first ds cDNA. In an embodiment, the isolated
production reaction site, e.g., production chamber, comprises primers that mediate the
production of the second ds cDNA.
In an embodiment, a cDNA strand that is complementary to a first mRNA that s an
XCVR from a cell is made by reverse transcription of the first mRNA. In an embodiment, a cDNA
strand that is complementary to a second mRNA that s a YCVR from a cell is made by reverse
transcription of the second mRNA.
In an embodiment, the reverse transcription takes place in the isolated production reaction
site, e.g., a production-micro chamber. In an embodiment, the reverse ription takes place in an
isolated cell reaction site, e. g., a cell isolation micro-chamber. In an embodiment, the reverse
transcription takes place outside the isolated production reaction site, e. g., a production micro-
chamber, or outside an ed cell reaction site, e. g., a cell isolation micro-chamber. In an
embodiment, the reverse transcription takes place e the isolated tion reaction site, e.g., a
production-micro chamber, and e an isolated cell reaction site, e.g., a cell isolation micro-
chamber. In an embodiment, the reverse transcription takes place outside an isolated reaction site,
e.g., outside a micro-chamber.
In an embodiment, the amplification comprises 30 or fewer cycles, e.g., 20 or fewer cycles,
e.g., 15 or fewer, 14 or fewer, 13 or fewer, 12 or fewer, 11 or fewer, 10 or fewer, 9 or fewer, 8 or
fewer, 7 or fewer, 6 or fewer, or 5 or fewer cycles.
In an ment, the reverse transcription and/or amplification uses one or more primers,
e. g., comprising a sequence specific for an XCVRS and/or a YCVRS.
In an embodiment, the reverse ription and/or amplification comprises using two or more
primers that e the production of the XC ds cDNA, wherein at least one primer comprises a
nucleotide modification, and wherein at least one primer does not se a nucleotide modification.
In an embodiment, the amplification comprises using two or more primers that mediate the production
of the YC ds cDNA, wherein at least one primer comprises a nucleotide modification, and n at
least one primer does not comprise a nucleotide modification.
In an embodiment, at least one primer ses a nucleotide modification, e.g., which
reduces, e. g., ts, DNA synthesis, e.g., by a DNA polymerase. In an embodiment, at least one
primer does not comprise a nucleotide modification, e.g., which reduces, e.g., inhibits, DNA
synthesis, 6.3., by a DNA polymerase.
In an embodiment, the nucleotide ation inhibits a DNA polymerase from extending
the DNA. Without wishing to be bound by theory, it is believed that in an embodiment any al
entity that reduces (e.g., blocks) DNA polymerase extension can be used in accordance with the
methods bed herein.
In an embodiment, the nucleotide modification is an insertion of a spacer to the primer, e. g.,
between two adjacent nucleotides in the primer. In an embodiment, the spacer is a flexible spacer. In
an embodiment, the spacer is a carbon spacer (e.g., n-, wherein n=3, 4, 5, 6, 7, 8, 9, 10, or
more), two or more (e.g., three, four, five, six, seven, eight, nine, ten, or more) abasic nucleotides, or a
polyethylene glycol (PEG) spacer. In an embodiment, the spacer is a PEG spacer. In an embodiment,
the nucleotide ation is 2’-O-methyl, 2’-OH, 2’-NH2, or uracil, e. g., to a ribose.
In an embodiment, the nucleotide modification is located internally or at the 3’ end of the
primer. In an embodiment, at least one primer comprises (i) a first member; (ii) a second member;
and optionally (iii) a third member, e.g., comprising a nucleotide modification described herein, e.g.,
located between (i) and (ii).
In an embodiment, the first member is capable of annealing with the second member. In an
embodiment, the first member is capable of ing with the second member in the same primer,
e.g., through intra-molecular hybridization, e.g., to form a hairpin structure comprising a duplex
region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more irs. In another
embodiment, the first member is capable of annealing hybridizing with the second member in a
different primer, e.g., through inter-molecular hybridization, e. g., to form a double-stranded structure
comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more
basepairs. Without wishing to be bound by theory, it is believed that in an ment, there are at
least two secondary structures that the modified primers can form and facilitate reduction (e.g.,
prevention) of competition to ate (e.g., bead) capture. For example, the secondary structure can
be a hairpin-like structure formed by intra-molecular hybridization (within the same primer), or the
secondary structure can be a duplex structure formed by inter-molecular hybridization (between two
different primers).
In an embodiment, the first member comprises a ce that is mentary to the
sequence of an ucleotide attached to the capture ate. In an ment, the second
member comprises (e.g., from 5’ to 3’) one, two, or all of: (i) a sequence that is complementary to at
least a portion of the first member; (ii) a universal priming ce (e.g., for PCR amplification or
next-generation sequencing); and (iii) a sequence complementary to a target sequence, e. g., an
XCVRS and/or a YCVRS. In an embodiment, the universal priming sequence is identical, or
substantially identical, to the sequence that is mentary to at least a portion of the first member.
In another embodiment, the universal priming sequence is different from the sequence that is
complementary to at least a portion of the first member. In an embodiment, the second member
comprises a sequence for homologous recombination (e.g., in a yeast or mammalian cell).
In an ment, at least one primer comprises a sequence encoding at least a portion of a
linker sequence, or a mentary sequence thereof. In an embodiment, the primer that comprises
a sequence encoding at least a portion of a linker sequence, or a complementary sequence thereof, is
orylated, e.g., 5 ’ phosphorylated. Without g to be bound by theory, it is believed that in
an embodiment, any ce with the general properties of flexibility (e.g., facilitated by glycine)
and hydrophilicity can work effectively in accordance with the methods described herein. Exemplary
linkers can generally have overrepresentation of one or more of Gly, Ser, Thr, or Ala and
underrepresentation of hydrophobic residues, e.g., one or more of Trp, Tyr, Phe, Cys, Met, Leu, or Ile.
The length of the primer may vary, e.g., 3-50 amino acid residues (e.g., 5-45, 10-40, 15-35, 20-30, 10-
20, 10-30, 20-40, or 30-40 amino acid residues). In an embodiment, the linker sequence comprises, or
consists of, ((Gly)m-Ser))n, where m=3, 4, 5, or more and n=1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more. In an
embodiment, the linker sequence comprises, or consists of, (Gly-Gly-Gly-Gly-Ser)n, where n=1, 2, 3,
4, 5, 6, 7, 8, 9, 10, or more.
In an embodiment, the primer is a primer described , e.g., in Examples.
In an embodiment, the reverse ription, the cation, or both, occurs in a solution in
the isolated production reaction site, e. g., production micro-chamber. In an embodiment, the e
transcription, the amplification, or both, does not occur on the substrate (e.g., bead). For example, the
e transcription, the amplification, or both, can occur on in a solution within a droplet.
In an ment, the XC ds cDNA comprises a 5’ overhang, e.g., a 5’ overhang that is
capable of hybridizing to an oligonucleotide attached to a capture substrate. In an embodiment, the
KC ds cDNA comprises a blunt end, e.g., a blunt end comprising a 5’ phosphate. In an embodiment,
the YC ds cDNA comprises a 5’ overhang, e.g., a 5’ overhang that is capable of hybridizing to an
oligonucleotide attached to a capture substrate. In an embodiment, the YC ds cDNA comprises a
blunt end, 6.3., a blunt end comprising a 5’ phosphate. In an embodiment, the XC ds cDNA and the
YC ds cDNA comprise sticky ends, e.g., both have 5’ overhangs.
In an embodiment, the XC strand and the YC strand are covalently linked, e.g., ligated, to
produce a single stranded nucleic acid sequence, wherein the XC and YC strands are both sense
strands or both antisense strands. In an embodiment, a denatured XC strand of the XC ds cDNA to a
denatured YC strand of the YC ds cDNA are covalently linked, e. g., ligated, wherein the XC and YC
s are both sense strands or both antisense strands. In an embodiment, the XC strand is present
in the XC ds cDNA and the YC strand is present in the YC ds cDNA, and wherein the XC ds cDNA
and the YC ds cDNA are covalently linked, e.g., ligated, e.g., to produce a double stranded nucleic
acid sequence.
In an embodiment, the covalent linking, e.g., ligation, occurs in the isolated tion
reaction site. In an embodiment, the isolated tion reaction site, e.g., a tion micro-
chamber, or the isolated linkage reaction site, e.g., a e micro-chamber, comprises a reagent that
is capable of covalently linking, e.g., ng, the XC and YC strands or the XC and YC ds cDNAs.
In an embodiment, the isolated production on site, e.g., a production micro-chamber comprises
an enzyme that covalently couples the XC and YC s or the XC and YC ds cDNAs. In an
embodiment, the enzyme is a ligase, e.g., a thermal stable ligase. In an embodiment, the covalent
linking ses ligase thermocycling.
In an embodiment, the covalent linking, e.g., on, occurs in a site different from the
isolated production on site, e.g., occurs in an isolated linkage reaction site, e.g., a linkage micro-
chamber. In an embodiment, the XC strand and the YC strand are transferred from the isolated
production site to the isolated linkage reaction site, e.g., a linkage micro-chamber, and the covalent
linking occurs in the isolated e reaction site, e.g., a linkage micro-chamber. In an embodiment,
the isolated linkage reaction site, e.g., a linkage micro-chamber, comprises a reagent that is capable of
covalently linking, e.g., ligating, the XC and YC strands or the XC and YC ds cDNAs. In an
embodiment, the ed linkage reaction site, e.g., a linkage micro-chamber, comprises an enzyme
that covalently couples the XC and YC s or the XC and YC ds cDNAs. In an embodiment, the
enzyme is a ligase, e.g., a thermal stable ligase. In an embodiment, the nt linking ses
ligase thermocycling.
In an ment, the covalent linking, e. g., ligation, comprises: (a) g the isolated
linkage reaction site, e.g., the linkage micro-chamber, under conditions (e.g., at 950C) that allow
ration of the XC strand and the YC strand; (b) cooling the isolated linkage reaction site, e. g.,
the e micro-chamber, under ions (e.g., at 50-65°C) that allow hybridization of the splint
oligonucleotide to the XC strand and the YC strand; (c) maintaining the isolated linkage reaction site,
e.g., the linkage micro-chamber, under conditions (e.g., at 45-65°C) that allow ligation of the XC
strand and the YC strand (e.g., formation of odiester bond between the XC strand and the YC
strand); and (d) repeating steps (a), (b), and (c) tially for 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50,
or more cycles.
In an embodiment, the XC strand and the YC strand are covalently linked, e.g., ligated, in the
presence of a splint oligonucleotide. In an embodiment, the splint oligonucleotide is hybridized to a
sequence comprising the junction of the XC strand and the YC strand, or a sequence complementary
thereof, and forms a duplexed region at the site of ligation. In an embodiment, the splint
oligonucleotide comprises a modification (e.g., an NHZ group) that inhibits DNA synthesis, e.g., by a
DNA polymerase. In an embodiment, the modification is at the 3’ end of the splint oligonucleotide.
In an embodiment, a strand complimentary to the covalently linked, e. g., ligated, XC and YC
strands is produced by amplification.
In an ment, the method, e.g., the step of covalent linkage, does not include a step of
overlap extension polymerase chain reaction (OE-PCR), also known as splicing by overlap ion
or splicing by overhang extension (SOE) PCR.
In an embodiment, the method further comprises, prior to acquiring the isolated production
reaction site, e.g., a production micro-chamber, acquiring an mRNA loaded capture ate.
In an embodiment, ing the mRNA loaded capture substrate comprising: a) acquiring an
isolated cell reaction site, e.g., a cell isolation micro-chamber, comprising: i) a cell; and ii) a capture
substrate capable of binding a first mRNA encoding an XCVR from the cell and a second mRNA
encoding a YCVR from the cell; and b) maintaining the isolated cell reaction site, e.g., the cell
isolation micro-chamber, under conditions that allow lysis of the cell and binding of the capture
substrate with the first mRNA and the second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a c
acid encoding an XCVR or a YCVR from a cell other than the cell (e.g., a different cell).
In an embodiment, the isolated cell reaction site, e. g., cell isolation micro-chamber, comprises
a lysing reagent, e.g., a detergent. In an embodiment, the cell is lysed by heat or an enzyme. In an
embodiment, the capture substrate ses a moiety (e.g., an oligonucleotide) which binds mRNA,
e.g., an oligo(dT).
In an embodiment, the method further comprises releasing the mRNA loaded capture
substrate from the isolated cell reaction site, e.g., the cell isolation micro-chamber. In an
embodiment, the releasing step is performed in the presence of a poly(dA) or poly(dT)
oligonucleotide, e.g., to reduce binding of non-captured mRNA.
In an embodiment, the mRNA loaded capture ate is transferred from the isolated cell
reaction site, e.g., the cell isolation micro-chamber, to the ed production reaction site, e.g., the
tion micro-chamber.
In an ment, the method further ses releasing the nucleic acid sequence from the
isolated production reaction site, e.g., the production micro-chamber. In an embodiment, the method
further comprises amplifying the nucleic acid sequence. In an embodiment, amplification of the
nucleic acid sequence occurs outside the isolated production reaction site, e.g., the production micro-
chamber, e. g., after the nucleic acid is ed from the ed production reaction site, e. g., the
tion micro-chamber. In an embodiment, amplification of the nucleic acid sequence occurs at
the isolated production on site, e.g., the production micro-chamber.
In an embodiment, the method further comprises sequencing all or a portion of the c
acid sequence.
In an embodiment, the method further ses inserting all or a portion of nucleic acid
sequence into a vector. In an embodiment, the vector supplies an additional XC element or YC
element not included in the nucleic acid sequence. In an embodiment, the vector supplies an XC
CDRl, an XC CDR2, or both. In an embodiment, the method further comprises sing the
vector.
In an embodiment, the method further comprises expressing the nucleic acid sequence to
produce a polypeptide comprising a segment that encodes an XC element of the XCVR, e.g., an
XCVRS, and a segment that encodes a YC element of the YCVR, e.g., a YCVRS. In an embodiment,
the YC element is N—terminal to the KC element in the polypeptide. In an embodiment, the XC
element is C-terminal to the YC element in the polypeptide.
In an embodiment, the method further comprises contacting the polypeptide with an antigen.
In an embodiment, the method further comprises determining if the polypeptide binds the n, in
vitro, ex vivo, or in viva, e.g., by a method or assay described herein.
In an aspect, the disclosure features a method of making a nucleic acid sequence comprising a
sequence that encodes an X chain element (XC t) of an antibody heavy chain variable region
(XCVR) and a Y chain element (YC element) of an antibody light chain variable region (YCVR), and
wherein the XCVR and YCVR are matched, comprising:
a) acquiring an isolated cell reaction site (e.g., an isolated cell reaction site bed herein),
e.g., a cell isolation micro-chamber, comprising: i) a cell (e.g., a cell described herein); and ii) a
capture substrate (e. g., a capture substrate described herein) capable of g a first mRNA
ng an XCVR from the cell and a second mRNA encoding a YCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form an mRNA loaded capture substrate,
wherein the isolated cell on site, e.g., cell isolation chamber, does not include a
nucleic acid encoding an XCVR or a YCVR from a cell other than the cell (e.g., a different cell);
c) contacting the mRNA loaded capture substrate with a reaction mixture, e.g., a reaction
mixture sing reverse transcriptase, that uses the loaded mRNA as a template to make cDNA
(this can occur, e.g., in the isolated cell reaction site, in an isolated production reaction site, or in
neither, e.g., not in an isolated on site);
(1) acquiring an isolated production reaction site (e.g., an isolated production reaction site
described herein), e.g., a production chamber, comprising: i) an X chain (XC) strand, n
the XC strand is a strand of an X chain double-stranded cDNA (XC ds cDNA) comprising a t
that encodes an XC element of the XCVR from the cell, e.g., an X chain variable region sequence
(XCVRS); and ii) a Y chain (YC) strand, wherein the YC strand is a strand of a Y chain double-
stranded cDNA (YC ds cDNA) comprising a segment that encodes a YC element of the YCVR from
the cell, e.g., a Y chain variable region sequence (YCVRS),
wherein the isolated tion on site, e.g., a production micro-chamber, does not
e a nucleic acid encoding a YCVR or an XCVR from a cell other than the cell (e. g., a different
cell); and
e) covalent linking, e. g., ligation, of the XC strand to the YC strand.
In an embodiment, one or more (e.g., two, three, four, or all) of the steps a)-e) are performed
in accordance with a method described herein. In an embodiment, each of the steps a)-e) is performed
in accordance with a method described herein.
In an , the disclosure features a method of making a nucleic acid sequence comprising a
sequence that encodes an X chain element (XC element) of an dy heavy chain variable region
(XCVR) and a Y chain element (YC element) of an antibody light chain variable region (YCVR), and
wherein the XCVR and YCVR are matched, comprising:
a) acquiring an isolated cell reaction site (e.g., an isolated cell reaction site described herein),
e.g., a cell isolation micro-chamber, comprising: i) a cell (e.g., a cell described herein); and ii) a
capture ate (e.g., a capture substrate described herein) capable of binding a first mRNA
encoding an XCVR from the cell and a second mRNA encoding a YCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and g of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not e a
nucleic acid encoding an XCVR or a YCVR from a cell other than the cell (e.g., a different cell);
c) acquiring an isolated production reaction site (e.g., an isolated production reaction site
described herein), e. g., a production micro-chamber, comprises: contacting the mRNA loaded capture
substrate with a on mixture, e.g., a reaction mixture comprising reverse riptase, that uses
the loaded mRNA as a template, to produce: a first double-stranded cDNA (ds cDNA) comprising a
strand that is complementary to a first mRNA that encodes an XCVR from a cell; and a second ds
cDNA comprising a strand complementary to a second mRNA ng a YCVR from the cell (the
cDNA loaded e substrate);
wherein the isolated production reaction site, e.g., a production chamber, does not
include a nucleic acid encoding a YCVR or an XCVR from a cell other than the cell (e.g., a different
cell).
(1) maintaining the isolated production reaction site, e.g., the production micro-chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce: a ity of
XC ds cDNAs sing a segment that encodes an XC element of the XCVR from the cell, e.g., an
XCVRS; and a plurality of YC ds cDNAs comprising a t that encodes a YC element of the
YCVR from the cell, e.g., a YCVRS;
e) acquiring an isolated linkage on site (e.g., an isolated linkage reaction site described
), e. g., a linkage micro-chamber, comprising: covalent linking, e. g., ligation, of a strand of the
KC ds cDNA (XC strand) to a strand of the YC ds cDNA (YC strand), wherein the XC and YC
strands are both sense strands or antisense strands; and
f) amplifying the covalently linked, e.g., ligated, KC and YC strands.
In an embodiment, one or more (e.g., two, three, four, five, or all) of the steps a)-f) are
performed in accordance with a method bed herein. In an embodiment, each of the steps a)-f) is
performed in accordance with a method described herein.
In an aspect, the disclosure features a method of making a library comprising a plurality of
unique members, the method comprising:
making the plurality of members, wherein each of the members comprises a sequence that
encodes an X chain element (XC element) of an X chain variable region (XCVR) and a Y chain
element (YC element) of a Y chain variable region (YCVR), and wherein the XCVR and YCVR are
matched, made by a method bed herein,
n each unique nucleic acid sequence of the plurality comprises an XC element and a
YC element from a different unique cell (e.g., a cell described herein),
thereby making a library comprising a plurality of unique members.
In an embodiment, the plurality of unique members comprises at least 104, 105, 106, 107, 108,
or 109 unique members. In an embodiment, the plurality of unique s comprises 104 to 109, 104
to 108, 104 to 107, 104 to 106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 108, 106 to
107, 104 to 105, 105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique members. In an embodiment, at
least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the s in the library are unique
members (which encode matched XC element and YC elements sequences). In an embodiment, less
than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the members in the library are unique members
(which encode matched XC element and YC ts sequences).
In an aspect, the disclosure features a library comprising a plurality of unique members,
wherein,
i) each unique member of the plurality comprises a segment that encodes an XC element, e.g.,
an XCVRS, and a segment that encodes a YC element, e.g., a YCVRS, n the XC element and
the YC element in each unique member is matched;
ii) each unique member of the plurality comprises a segment that s an XC element,
e.g., an XCVRS, and a segment that encodes a YC element, e.g., a YCVRS, from a ent unique
cell; and
iii) the library comprises one or more (e.g., two, three, four, or all) of the following
ties:
a) the library is made by a method described ;
b) the plurality of unique members comprises at least 104, 105, 106, 107, 108, or 109
unique nucleic acid sequences;
c) the plurality of unique members comprises 104 to 109, 104 to 108, 104 to 107, 104 to
106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 108, 106 to 107, 104 to 105,
105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique members;
d) at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the s in
the library are unique members (which encode matched XC t and YC elements
sequences); or
e) less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the members in the library
are unique members (which encode matched XC element and YC elements sequences).
In an embodiment, each unique member of the plurality is configured such that, when
expressed, the XC element, e. g., the XCVRS, and the YC element, e.g., the YCVRS, form a
functional antigen binding molecule, e.g., an scFV.
In an embodiment, the y is a display library. In an embodiment, each of the members of
the plurality further encodes a polypeptide that results in display of the member on the surface of a
display entity. In an embodiment, the library is a phage display library. In an ment, the
y is a yeast y library. In an embodiment, the library is a mammalian display library.
In an aspect, the disclosure features a method of making a binding polypeptide (e.g., a
polypeptide comprising an XC element and a YC element), the method comprising: a) acquiring a
library described herein, e.g., by a method described herein; and b) expressing a polypeptide encoded
by a unique nucleic acid of the library.
In an embodiment, the method further ses contacting the polypeptide with an n.
In an embodiment, the method further comprises retrieving (e.g., isolating or purifying) the nucleic
acid that encodes a polypeptide that binds the n.
In an aspect, the disclosure features an isolated production reaction site, e.g., a production
micro-chamber, which is an isolated tion reaction site described herein (e.g., comprising a
nucleic acid encoding an XCVR and a nucleic acid encoding a YCVR, wherein the XCVR and the
YCVR are matched).
In an embodiment, the ed production reaction site, e.g., a production micro-chamber,
does not include a nucleic acid encoding an XCVR or a YCVR from a different cell.
In an ment, the isolated production reaction site, e.g., a production micro-chamber,
comprises one, two, or all of: (i) one or more primers specific to V gene ces of the XC and YC;
(ii) one or more s specific to overhangs introduced onto the XC and YC cDNAs; or (iii) one or
more primers comprising a first member, a second member, and a third member comprising a
nucleotide modification (e.g., a spacer) located between the first and second members, wherein the
first member is capable of annealing with the second member of the same primer or a different
primer, e.g., forming a structure comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,
l6, l7, l8, 19, 20, more basepairs.
In an embodiment, the isolated production reaction site, e.g., a tion micro-chamber,
does not comprise a reagent that can covalently link nucleic acids, e.g., a ligase, e.g., a thermostable
ligase. In another embodiment, the isolated production reaction site, e. g., a tion micro-
chamber, comprises a reagent that can covalently link nucleic acids, e.g., a ligase, e.g., a thermostable
ligase.
In an aspect, the disclosure features a self-annealing oligonucleotide comprising a first
member, a second member, and third member comprising a nucleotide modification (e. g., a )
located between the first and second s, wherein the first member is capable of ing with
the second member of the same oligonucleotide (e.g., for a method of making a nucleic acid sequence
comprising a ce that encodes an XC element of an XCVR and a YC element of a YCVR,
wherein the XCVR and YCVR are matched).
In an embodiment, the first and second members are capable of forming a hairpin structure
comprising a duplex region of 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more
basepairs. In an embodiment, the first member is 5-40 nucleotides, e. 3., 5-10, 5-20, 5-30, 30-40, 20-
40, 10-30, 10-30, or 15-25 nucleotides, in length. In an embodiment, the second member is 5-40
nucleotides, e.g., 5-10, 5-20, 5-30, 30-40, 20-40, 10-30, 10-30, or 15-25 nucleotides, in length.
In an embodiment, the spacer is a spacer bed herein, e.g., a flexible spacer or a PEG
spacer.
In an embodiment, the first member comprises a sequence that is complementary to the
sequence of an oligonucleotide attached to a capture substrate.
In an embodiment, the second member comprises (e.g., from 5’ to 3’) one, two, or all of: (i) a
ce that is complementary to at least a portion of the first member; (ii) a universal priming
sequence (e.g., for PCR amplification or next-generation sequencing); and (iii) a sequence
complementary to a target sequence, e.g., an XCVRS and/or a YCVRS. In an embodiment, the
universal priming sequence is identical, or substantially identical, to the sequence that is
complementary to at least a portion of the first member. In another embodiment, the universal
g sequence is different from the sequence that is complementary to at least a portion of the first
member. In an embodiment, the second member comprises a sequence for homologous
recombination (e.g., in a yeast or mammalian cell).
In an , the disclosure features an isolated linkage reaction site, e. g., a linkage micro-
chamber, which is an ed e reaction site described herein (e.g., sing a nucleic acid
encoding an XCVR and a nucleic acid encoding a YCVR, n the XCVR and the YCVR are
matched).
In an embodiment, the isolated linkage reaction site, e.g., a linkage micro-chamber, does not
include a nucleic acid encoding an XCVR or a YCVR from a different cell.
In an ment, the ed linkage reaction site, e.g., a e micro-chamber, comprises
a splint oligonucleotide (e.g., a splint ucleotide described herein) that is capable of hybridizing
to a sequence comprising the junction of the XC strand and the YC strand, or a sequence
mentary thereof, to form a duplexed region at the site of ligation.
In an embodiment, the isolated e reaction site, e.g., a linkage micro-chamber, comprises
a reagent that can covalently link nucleic acids, e.g., a ligase, e.g., a thermostable ligase.
T-Cell Receptor Molecules
The disclosures herein are not intended to be limited to antibody molecules. In an
embodiment, the binding molecule is a TCR molecule, e. g., a soluble TCR molecule. In an
embodiment, the binding molecule comprises a TCR or chain variable region and a TCR [3 chain
variable . In an embodiment, the binding molecule comprises a TCR 7 chain variable region
and a TCR 5 chain variable region. For example, in any of the aspects, embodiments, and definitions
herein, an antibody heavy chain (or variable region) can be ed with a TCR or chain (or variable
region), and an antibody light chain (or variable region) can be ed with a TCR [3 chain (or
variable region); or an antibody heavy chain (or variable region) can be replaced with a TCR 7 chain
(or variable region), and an antibody light chain (or variable region) can be replaced with a TCR 5
chain (or variable region).
Disclosed herein are T-cell receptor (TCR) molecules and libraries of TCR molecules. In an
embodiment, the TCR molecule or y of TCR molecules are made by a method described herein.
As used herein, the term “TCR molecule,” also known as “T-cell receptor molecule” or “T
cell receptor le,” refers to a protein, e.g., a TCR chain or a fragment thereof, comprising at
least one TCR variable domain sequence. The term “TCR molecule” es, for example, full-
, mature TCRs and antigen-binding nts of a TCR. For example, a TCR molecule can
include an 0t chain variable domain sequence and a B chain variable domain sequence. In another
example, a TCR molecule can include a y chain variable domain sequence and a 6 chain variable
domain sequence. In an embodiment, the TCR molecule is a soluble TCR molecule.
T-cell receptors can be found on the surface of T cells and are responsible for recognizing
fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. A
TCR lly e two different protein chains. In humans, in about 95% of T cells the TCR
include an alpha (or) chain and a beta ([3) chain (encoded by TRA and TRB, respectively), whereas in
about 5% of T cells the TCR include gamma and delta (7/5) chains (encoded by TRG and TRD,
respectively). This ratio can change during ontogeny and in diseased states (such as leukemia).
When the TCR engages with antigenic peptide and MHC (peptide/MHC), the T lymphocyte is
activated through signal transduction, e.g., a series of biochemical events mediated by associated
enzymes, eptors, specialized r molecules, and activated or released transcription factors.
For example, the naturally-occurring TCR is typically a disulfide-linked ne-anchored
heterodimeric protein including, e.g., the highly variable alpha (or) and beta ([3) chains expressed as
part of a complex with the invariant CD3 chain molecules. T cells sing this receptor are
sometimes referred to as azfi (or (XB) T cells, though a minority of T cells express an alternate or,
formed by variable gamma (y) and delta (6) chains, sometimes referred as 1/5 T cells.
Each chain of TCR can include two extracellular domains: a variable (V) region and a
constant (C) region. The constant region is proximal to the cell membrane, followed by a
embrane region and a short cytoplasmic tail, while the variable region can bind to the
peptide/MHC complex.
The variable domain of the TCR or chain or [5 chain each can have three hypervariable or
mentarity determining regions (CDRs). There can also be an additional area of
hypervariability on the [5 chain (HV4), which typically does not contact antigen and is not considered
a CDR. The es in these variable domains are located in two regions of the TCR, at the interface
of the or and [3 chains and in the [3-chain framework region that is in proximity to the CD3 signal-
transduction complex. Without wishing to be bound by theory, it is believed that in an embodiment,
CDR3 is the main CDR responsible for recognizing sed antigen. CDRl of the alpha chain can
interact with the N—terminal part of the antigenic peptide, and CDRl of the [3 chain can interact with
the C-terminal part of the peptide. CDR2 may recognize the MHC. CDR4 of the [3 chain is generally
not thought to participate in n recognition, but may ct with ntigens.
The constant domain of the TCR include, e.g., short connecting sequences, which form a link
between the two chains, e.g., h disulfide bonds.
The generation of TCR diversity arises mainly from genetic recombination of the DNA
encoded segments in dual somatic T cells by somatic V(D)J recombination . Each recombined
TCR may possess unique antigen specificity, determined by the ure of the antigen-binding site,
e.g., formed by the 0t and [3 chains in case of (113 T cells or y and 5 chains on case of 1/5 T cells. For
example, the TCR 0c and y chains can be generated by VJ recombination, and the [3 and 5 chains can
be generated by VDJ recombination. The ection of these specific regions (e.g., V and J for 0t or
7 chain; V, D, and J for [3 or 5 chain) corresponds to the CDR3 region that is typically ant for
peptide/MHC recognition.
The TCR receptor can form a complex of variable TCR chains (e.g., 0t and [3 chains with three
dimeric signaling s CD35/e, CD3~yle and CD247 C/C or Cjn). T cell can express clonal TCRs
which recognize specific peptide/MHC complex during physical contact between T cell and antigen-
presenting cell-APC (MHC class II) or any other cell type (MHC class I). The signal from the T-cell
complex can be enhanced by simultaneous binding of the MHC molecules by a ic co-receptor.
For example, on helper T cells and regulatory T cells, the co-receptor is CD4 that is specific for MHC
class II, and on xic T cells, the co-receptor is CD8 that is specific for MHC class I.
The term “TCR” includes intact molecules as well as functional fragments thereof. TCR
fragments may be obtained using any suitable method, ing several conventional techniques
known to those with skill in the art, and the fragments can be screened for utility in the same manner
as are intact TCRs. Constant s of the TCRs can be altered, e.g., mutated, to modify the
properties of the TCR.
The TCR molecule can be a single chain TCR. The single chain TCR can be dimerized or
multimerized to generate multivalent TCRs having icities for different epitopes of the same
target protein.
The TCR molecules disclosed herein can also be single domain TCRs. Single domain TCRs
can include TCRs whose complementary determining regions are part of a single domain polypeptide.
Examples include, but are not limited to, or, [3, y, or 5 chain TCRs, TCRs naturally devoid of a 0t, [3, y,
or 5 chain, single domain TCRs derived from conventional two-chain TCRs, engineered TCRs and
single domain scaffolds other than those derived from TCRs. Single domain TCRs may be any of the
art, or any future single domain TCRs. Single domain TCRs may be derived from any species
including, but not limited to mouse, human, camel, llama, fish, shark, goat, rabbit, and bovine.
The variable regions can be subdivided into regions of hypervariability, termed
ementarity determining regions” (CDR), interspersed with regions that are more conserved,
termed “framework regions” (FR or FW). The terms “complementarity determining ,” and
“CDR,” as used herein in the context of TCR molecules refer to the sequences of amino acids within
TCR variable s which confer antigen specificity and binding affinity. As used herein, the terms
“framework,” “FW” and “FR” are used interchangeably.
As used herein, a “TCR variable domain sequence” refers to an amino acid sequence which
can form the structure of a TCR variable domain. For e, the sequence may include all or part
of the amino acid sequence of a naturally-occurring variable domain. For example, the sequence may
or may not include one, two, or more N— or C-terminal amino acids, or may include other alterations
that are compatible with ion of the protein structure.
The term “antigen-binding region” refers to the part of a TCR molecule that comprises
determinants that form an interface that binds to an antigen, or an epitope thereof. With respect to
proteins (or n mimetics), the antigen-binding region lly includes one or more loops (of at
least, e.g., four amino acids or amino acid mimics) that form an ace that binds to the antigen.
Typically, the antigen-binding region of a TCR molecule includes at least one or two CDRs and/or
hypervariable loops, or more typically at least three, four, five or six CDRs and/or hypervariable
loops.
The terms “compete” or “cross-compete” are used interchangeably herein to refer to the
ability of a TCR molecule to interfere with binding of another TCR molecule, to a . The
interference with binding can be direct or indirect (e.g., h an allosteric modulation of the TCR
molecule or the target). The extent to which an antibody molecule is able to interfere with the binding
of another TCR molecule to the target, and therefore whether it can be said to compete, can be
determined using a competition binding assay, for example, a FACS assay, an ELISA or BIACORE
assay. In an embodiment, a competition binding assay is a quantitative ition assay. In an
embodiment, a first TCR molecule is said to compete for g to the target with a second TCR
le when the binding of the first antibody molecule to the target is reduced by 10% or more,
e.g., 20% or more, 30% or more, 40% or more, 50% or more, 55% or more, 60% or more, 65% or
more, 70% or more, 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 98% or
more, 99% or more in a competition binding assay (e.g., a competition assay described herein).
The TCR molecule can be a polyclonal or a monoclonal. In some embodiments, the TCR can
be recombinantly produced, e.g., produced by any suitable phage display or combinatorial methods.
Phage y and combinatorial methods are known in the art.
In an embodiment, the TCR molecule is a fully human TCR (e. g., a TCR made in a mouse
which has been genetically engineered to produce a TCR from a human TCR sequence), or a non-
human TCR, e.g., a rodent (mouse or rat), goat, e (e.g., monkey), camel TCR. In an
embodiment, the non-human TCR is a rodent (mouse or rat TCR). For example, Human TCRs can be
generated using transgenic mice carrying the human TCR genes rather than the mouse system.
A TCR can be one in which the variable region, or a portion thereof, e.g., the CDRs, are
generated in a non-human organism, e.g., a rat or mouse. Chimeric, CDR-grafted, and humanized
TCRs are within the invention. TCRs generated in a non-human sm, e.g., a rat or mouse, and
then modified, e.g., in the variable framework or constant region, to decrease antigenicity in a human
are within the invention.
Chimeric TCRs can be produced by any suitable recombinant DNA technique.
A humanized or CDR-grafted TCR will have at least one or two but generally all three
recipient CDRs (of TCR chains) replaced with a donor CDR. The TCR may be replaced with at least
a portion of a non-human CDR or only some of the CDRs may be replaced with non-human CDRs. It
is only necessary to replace the number of CDRs required for g of the humanized TCR to an
antigen. In an embodiment, the donor will be a rodent TCR, e.g., a rat or mouse TCR, and the
ent will be a human framework or a human consensus framework. Typically, the TCR
providing the CDRs is called the “donor” and the TCR providing the ork is called the
tor.” In some embodiments, the donor TCR is a non-human (e.g., rodent). The acceptor
framework is lly a naturally-occurring (e.g., a human) framework or a sus framework, or
a sequence about 85% or higher, e.g., 90%, 95%, 99% or higher identical thereto.
As used herein, the term “consensus sequence” refers to the ce formed from the most
frequently occurring amino acids (or nucleotides) in a family of related sequences (See e.g., Winnaker,
From Genes to Clones (Verlagsgesellschaft, Weinheim, Germany 1987). In a family of proteins, each
position in the sus sequence is ed by the amino acid occurring most frequently at that
position in the family. If two amino acids occur equally frequently, either can be included in the
consensus sequence. A “consensus framework” refers to the framework region in the consensus TCR
sequence.
A TCR can be humanized by any suitable method. Humanized or CDR-grafted TCR s can be
produced by CDR-grafting or CDR substitution, wherein one, two, or all CDRs of an immunoglobulin
chain can be replaced. Also provided are humanized TCRs in which specific amino acids have been
substituted, deleted or added.
In an embodiment, the TCR molecule has a nt region. The constant region can be
d, e.g., mutated, to modify a property of the TCR molecule. In an embodiment, a constant
region of the TCR molecule is altered. Methods for altering a constant region are known in the art.
In an embodiment, the only amino acids in the TCR molecule are cal amino acids. In
an ment, the TCR molecule comprises naturally-occurring amino acids; analogs, derivatives
and congeners thereof; amino acid analogs having variant side chains; and/or all stereoisomers of any
of any of the ing. The TCR molecule may comprise the D- or L— optical isomers of amino
acids and peptidomimetics.
A polypeptide of a TCR le described herein may be linear or branched, it may
comprise modified amino acids, and it may be interrupted by non-amino acids. The TCR molecule
may also be modified; for example, by disulfide bond formation, glycosylation, lipidation, acetylation,
phosphorylation, or any other manipulation, such as conjugation with a labeling component. The
polypeptide can be isolated from natural sources, can be a produced by recombinant techniques from
a otic or prokaryotic host, or can be a product of synthetic ures.
The TCR molecule described herein can be used alone in unconjugated form, or can be bound
to a substance, e.g., a toxin or moiety (e.g., a therapeutic drug; a compound emitting radiation;
molecules of plant, fungal, or bacterial origin; or a biological protein (e. g., a protein toxin) or le
(e. g., a recombinant viral particle, e.g., via a viral coat n). For example, the TCR molecule can
be coupled to a radioactive isotope such as an (1-, [3-, or y—emitter, or a B-and y—emitter.
A TCR molecule can be derivatized or linked to r functional molecule (e.g., another
peptide or protein). As used herein, a “derivatized” TCR le is one that has been modified.
Methods of derivatization include but are not limited to the addition of a fluorescent moiety, a
ucleotide, a toxin, an enzyme or an affinity ligand such as biotin. Accordingly, the TCR
molecules are intended to include derivatized and otherwise modified forms of the antibodies
described herein, including immunoadhesion molecules. For example, a TCR molecule can be
functionally linked (by chemical coupling, c fusion, noncovalent association or otherwise) to
one or more other lar entities, such as another TCR (e.g., a bispecific TCR), a detectable agent,
a toxin, a pharmaceutical agent, and/or a protein or peptide that can mediate association of the TCR or
TCR portion with another molecule (such as a streptavidin core region or a polyhistidine tag).
Some types of derivatized TCR le are produced by inking two or more TCRs (of
the same type or of different types, e.g., to create bispecific TCRs). Suitable crosslinkers include
those that are heterobifunctional, having two distinctly reactive groups separated by an appropriate
spacer (e.g., m—maleimidobenzoyl-N-hydroxysuccinimide ester) or homobifunctional (e.g.,
disuccinimidyl suberate). Such linkers are available from Pierce Chemical Company, Rockford, Ill.
Useful able agents with which a TCR molecule may be derivatized (or d) to
include fluorescent compounds, various enzymes, prosthetic groups, luminescent materials,
bioluminescent materials, fluorescent emitting metal atoms, e.g., um (Eu), and other anthanides,
and radioactive materials (described below). Exemplary cent detectable agents include
fluorescein, cein isothiocyanate, rhodamine, 5dimethylamine-l-napthalenesulfonyl chloride,
phycoerythrin and the like. A TCR may also be derivatized with detectable enzymes, such as alkaline
phosphatase, horseradish peroxidase, B-galactosidase, acetylcholinesterase, glucose oxidase and the
like. When a TCR is derivatized with a detectable enzyme, it is detected by adding additional reagents
that the enzyme uses to produce a able reaction product. For e, when the detectable
agent horseradish peroxidase is present, the addition of hydrogen peroxide and diaminobenzidine
leads to a colored reaction product, which is detectable. A TCR molecule may also be tized
with a prosthetic group (e.g., streptavidin/biotin and avidin/biotin). For example, a TCR may be
derivatized with biotin, and detected through indirect measurement of avidin or streptavidin binding.
Examples of suitable fluorescent materials include umbelliferone, cein, fluorescein
isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an
example of a luminescent al includes luminol; and es of bioluminescent materials
include luciferase, luciferin, and aequorin.
Labeled TCR molecules can be used, for example, diagnostically and/or experimentally in a
number of contexts, including (i) to isolate a predetermined antigen by standard techniques, such as
ty chromatography or immunoprecipitation; (ii) to detect a predetermined antigen (e.g., in a
cellular lysate or cell supernatant) in order to evaluate the abundance and pattern of expression of the
protein; (iii) to monitor protein levels in tissue as part of a clinical testing procedure, e.g., to determine
the efficacy of a given treatment regimen.
A TCR molecule may be ated to another molecular entity, typically a label or a
therapeutic (e.g., antimicrobial (e.g., antibacterial or bactericidal), immunomodulatory,
immunostimularoty, cytotoxic, or cytostatic) agent or moiety. Radioactive isotopes can be used in
stic or therapeutic applications. Radioactive isotopes that can be coupled to the antibody
molecules include, but are not limited to (1-, [3-, or y—emitters, or B-and y—emitters. Such ctive
isotopes include, but are not limited to iodine (1311 or I), yttrium (90Y), lutetium ), actinium
(225Ac), praseodymium, astatine , rhenium (186Re), bismuth (212Bi or 213Bi), indium (1 1 1In),
technetium (99mTc), orus (32F), rhodium (lgth), sulfur (35$) carbon (14C), tritium (3H),
chromium (SlCr), chlorine (36Cl), cobalt (“Co or 58C0), iron (59Fe), selenium (7586), or gallium (67Ga).
Radioisotopes useful as therapeutic agents include yttrium (90Y), lutetium (177Lu), actinium (225Ac),
praseodymium, astatine (leAt), rhenium ), bismuth (212Bi or 213Bi), and rhodium (lgth).
Radioisotopes useful as labels, e.g., for use in diagnostics, include iodine (1311 or 125I), indium (mln),
technetium (99mTc), phosphorus (32F), carbon (14C), and tritium (3H), or one or more of the eutic
isotopes listed above.
The present sure provides radiolabeled TCR molecules and s of labeling the
same. In an embodiment, a method of ng a TCR molecule is disclosed. The method includes
contacting a TCR molecule, with a chelating agent, to thereby produce a ated TCR. The
conjugated antibody is radiolabeled with a radioisotope, e.g., 111Indium, ium and 177Lutetium, to
thereby produce a labeled TCR molecule.
In some aspects, this disclosure es a method of making a TCR molecule disclosed
herein. The method includes: providing an antigen, or a fragment thereof; obtaining a TCR molecule
that specifically binds to the n; evaluating efficacy of the TCR molecule in ting activity
of the antigen and/or organism sing the antigen. The method can further include administering
the TCR molecule, including a derivative thereof (e.g., a humanized TCR molecule) to a subject, e.g.,
a human.
This disclosure provides an isolated c acid molecule encoding the above TCR molecule,
vectors and host cells thereof. The nucleic acid molecule includes, but is not limited to, RNA,
c DNA and cDNA.
Animal Models
The polypeptides (e.g., binding polypeptides, e.g., dy molecules or TCR molecules)
described herein can be evaluated in viva, e.g., using various animal models. For example, an animal
model can be used to test the efficacy of a binding polypeptide (e.g., an antibody molecule or TCR
molecule) bed herein in modulating a biological on of a target molecule or cell. As
another example, an animal model can also be used to test the efficacy of a binding polypeptide (e.g.,
an antibody molecule or TCR molecule) described herein in in treating, preventing, or diagnosing a
disorder described . Animal models can also be used, e.g., to igate for side effects,
measure concentrations of binding ptides (e.g., antibody molecules or TCR molecules) in situ,
demonstrate correlations between a function of a target molecule or cell and a disorder described
herein.
Exemplary animal models for other disorders described herein are also known in the art.
Exemplary types of animals that can be used to evaluate the binding polypeptides (e.g., antibody
molecules or TCR molecules) bed herein include, but are not limited to, mice, rats, rabbits,
guinea pigs, and monkeys.
Pharmaceutical Compositions and Kits
In some aspects, this disclosure provides compositions, e.g., pharmaceutically acceptable
compositions, which include a polypeptide (e.g., a binding polypeptide, e.g., an antibody molecule or
a TCR molecule) described herein, ated together with a ceutically acceptable carrier.
As used herein, “pharmaceutically acceptable carrier” includes any and all ts,
dispersion media, isotonic and absorption delaying agents, and the like that are physiologically
compatible. The r can be suitable for intravenous, intramuscular, subcutaneous, parenteral,
rectal, spinal or mal administration (e.g., by ion or infusion). In certain embodiments, less
than about 5%, e.g., less than about 4%, 3%, 2%, or 1% of the binding polypeptides in the
pharmaceutical composition are present as aggregates. In other embodiments, at least about 95%,
e.g., at least about 96%, 97%, 98%, 98.5%, 99%, 99.5%, 99.8%, or more of the binding polypeptides
in the pharmaceutical composition are present as monomers. In some embodiments, the level of
aggregates or monomers is ined by chromatography, e.g., high performance size exclusion
chromatography (HP-SEC).
The compositions set out herein may be in a variety of forms. These include, for example,
liquid, semi-solid and solid dosage forms, such as liquid solutions (e.g., injectable and infusible
solutions), dispersions or suspensions, liposomes, and suppositories. A le form depends on the
intended mode of administration and therapeutic application. Typical suitable compositions are in the
form of injectable or infusible ons. One suitable mode of stration is parenteral (e.g.,
intravenous, aneous, intraperitoneal, intramuscular). In an embodiment, the binding
polypeptide is administered by intravenous infusion or injection. In another embodiment, the binding
polypeptide is administered by intramuscular or subcutaneous injection.
The phrases “parenteral administration” and “administered parenterally” as used herein means
modes of administration other than enteral and topical stration, usually by injection, and
includes, without limitation, intravenous, intramuscular, rterial, intrathecal, apsular,
intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular,
intraarticular, subcapsular, subarachnoid, intraspinal, epidural and intrasternal injection and infusion.
Therapeutic compositions typically should be sterile and stable under the ions of
manufacture and storage. The composition can be ated as a solution, microemulsion,
dispersion, liposome, or other ordered structure suitable to high concentrations of binding
polypeptides (e.g., dy molecules or TCR molecules). Sterile injectable solutions can be
prepared by incorporating the active compound (e.g., binding polypeptide) in the required amount in
an appropriate solvent with one or a combination of ingredients enumerated above, as required,
followed by filtered sterilization. Generally, dispersions are prepared by incorporating the active
compound into a sterile vehicle that contains a basic dispersion medium and the required other
ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile
injectable ons, the preferred methods of preparation are vacuum drying and freeze-drying that
yields a powder of the active ingredient plus any additional desired ingredient from a previously
sterile-filtered solution thereof. The proper fluidity of a solution can be maintained, for example, by
the use of a coating such as in, by the maintenance of the ed particle size in the case of
dispersion and by the use of surfactants. ged absorption of injectable compositions can be
brought about by including in the composition an agent that delays absorption, for example,
monostearate salts and gelatin.
The binding polypeptides (e.g., antibody molecules or TCR ors) described herein can be
administered by a variety of methods. Several are known in the art, and for many therapeutic,
prophylactic, or stic applications, an appropriate mode of administration is intravenous
injection or infusion. For example, the binding polypeptides can be administered by intravenous
infusion at a rate of less than lOmg/min; preferably less than or equal to 5 mg/min to reach a dose of
about 1 to 100 mg/mz, preferably about 5 to 50 mg/m2, about 7 to 25 mg/m2 and more preferably,
about 10 mg/m2. As will be appreciated by the skilled artisan, the route and/or mode of
administration will vary ing upon the d results. In certain embodiments, the active
compound may be prepared with a carrier that will t the nd against rapid release, such
as a controlled release formulation, including implants, transdermal patches, and microencapsulated
delivery systems. Biodegradable, biocompatible rs can be used, such as ethylene vinyl acetate,
polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Many methods for
the preparation of such formulations are patented or generally known to those skilled in the art. See,
e.g., Sustained and Controlled Release Drug Delivery Systems, J. R. Robinson, ed., Marcel Dekker,
Inc., New York, 1978.
In certain embodiments, the binding polypeptide (e. g., antibody molecule or TCR molecule)
can be orally administered, for example, with an inert diluent or an assimilable edible carrier. The
binding polypeptide (and other ients, if desired) may also be enclosed in a hard or soft shell
gelatin capsule, compressed into tablets, or orated directly into the subject’s diet. For oral
therapeutic administration, the binding polypeptide may be incorporated with excipients and used in
the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, , ,
and the like. To administer the binding polypeptide (e. g., dy molecule) by other than parenteral
administration, it may be necessary to coat the compound with, or co-administer the nd with,
a material to prevent its inactivation. Therapeutic, prophylactic, or diagnostic compositions can also
be administered with medical devices, and several are known in the art.
Dosage regimens are adjusted to provide the d response (e. g., a eutic,
lactic, or diagnostic response). For e, a single bolus may be administered, several
divided doses may be administered over time or the dose may be proportionally reduced or increased
as indicated by the exigencies of the therapeutic situation. It is especially advantageous to formulate
parenteral compositions in dosage unit form for ease of administration and mity of dosage.
Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the
subjects to be treated; each unit contains a predetermined quantity of active compound calculated to
produce the desired therapeutic effect in association with the required pharmaceutical carrier. The
specification for the dosage unit forms are dictated by and directly dependent on (a) the unique
characteristics of the antibody molecule and the particular therapeutic, prophylactic, or stic
effect to be achieved, and (b) the limitations inherent in the art of compounding such a binding
polypeptide for the treatment of sensitivity in individuals.
An exemplary, non-limiting range for a therapeutically, prophylactically, or diagnostically
effective amount of a binding ptide (e. g., an antibody le or TCR molecule) is about 0.1-
50 mg/kg, e.g., about 0.1-30 mg/kg, e.g., about 1-30, 1-15, 1-10, 1-5, 5-10, or 1-3 mg/kg, e.g., about
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30, 40, or 50 mgikg. The binding polypeptide can be administered
by intravenous infusion at a rate of less than 10 mg/min, e. g., less than or equal to 5 mg/min to reach a
dose of about 1 to 100 mg/mz, e.g., about 5 to 50 mg/m2, about 7 to 25 mg/m2, e.g., about 10 mg/m2.
It is to be noted that dosage values may vary with the type and severity of the condition to be
ated. It is to be further understood that for any particular t, specific dosage regimens
should be adjusted over time according to the individual need and the professional judgment of the
person administering or supervising the administration of the compositions, and that dosage ranges set
forth herein are exemplary only and are not ed to limit the scope or practice of the claimed
compositions.
The pharmaceutical compositions herein may include a “therapeutically effective amount,”
“prophylactically effective amount,” or “diagnostically effectively amount” of a binding polypeptide
(e. g., an dy molecule or TCR molecule) described herein.
A “therapeutically effective amount” refers to an amount effective, at s and for periods
of time necessary, to e the desired therapeutic result. A therapeutically effective amount of the
binding polypeptide (e.g., antibody molecule or TCR molecule) may vary according to factors such as
the disease state, age, sex, and weight of the individual, and the ability of the antibody or antibody
n to elicit a desired response in the individual. A therapeutically ive amount is also one in
which any toxic or ental effect of the antibody molecule is outweighed by the therapeutically
beneficial effects.
A “therapeutically effective dosage” typically inhibits a measurable parameter by at least
about 20%, e.g., by at least about 40%, by at least about 60%, or by at least about 80% relative to
untreated subjects. The measurable ter may be, e.g., hematuria, colored urine, foamy urine,
pain, swelling (edema) in the hands and feet, or high blood pressure. The ability of a binding
polypeptide (e.g., an dy molecule) to inhibit a able parameter can be evaluated in an
animal model system predictive of efficacy in treating or preventing a disorder described herein.
Alternatively, this property of a composition can be evaluated by examining the ability of the binding
polypeptide (e.g., antibody molecule or TCR molecule) to modulate a biological function of a target
molecule or cell, e.g., by an in vitro assay.
A “prophylactically effective amount” refers to an amount effective, at s and for
periods of time necessary, to achieve the desired prophylactic result. Typically, since a prophylactic
dose is used in subjects prior to or at an earlier stage of disease, the prophylactically effective amount
will be less than the therapeutically effective amount.
A “diagnostically effective amount” refers to an amount effective, at dosages and for periods
of time necessary, to achieve the desired diagnostic result. Typically, a diagnostically effective
amount is one in which a disorder, e.g., a er described herein, can be diagnosed in vitro, ex vivo,
or in viva.
Also within this sure is a kit that comprises a binding polypeptide (e. g., an antibody
molecule or TCR molecule), described herein. The kit can include one or more other elements
including: instructions for use; other ts, e.g., a label, a therapeutic agent, or an agent useful for
chelating, or otherwise coupling, an dy molecule to a label or eutic agent, or a
radioprotective composition; devices or other materials for preparing the binding polypeptide (e.g.,
antibody molecule or TCR molecule) for administration; pharmaceutically acceptable carriers; and
devices or other materials for administration to a subject.
Nucleic Acids
The t disclosure also features nucleic acids sing nucleotide sequences that
encode polypeptides (e. g., binding polypeptides, e.g., dy molecules or T cell receptor
molecules), as described herein.
In an embodiment, the c acid further comprises a nucleotide sequence ng a heavy
chain variable region of a polypeptide (6.3., an antibody molecule or TCR molecule) bed herein,
or having a tide sequence substantially homologous thereto (e.g., a sequence at least about
85%, 90%, 95%, 99% or more identical thereto, and/or e of hybridizing under the stringency
conditions described herein). In another embodiment, the nucleic acid further comprises a nucleotide
sequence encoding a light chain variable region of a polypeptide (e.g., an antibody molecule or TCR
molecule) described herein, or a nucleotide sequence substantially homologous thereto (e.g., a
sequence at least about 85%, 90%, 95%, 99% or more identical thereto, and/or capable of izing
under the stringency conditions described herein). In yet another embodiment, the nucleic acid
further comprises a tide sequence encoding a heavy chain variable region and a light chain
variable region of a polypeptide (e.g., an antibody molecule or TCR molecule) described herein, or a
nucleotide sequence substantially homologous thereto (e.g., a sequence at least about 85%, 90%, 95%,
99% or more identical thereto, and/or capable of hybridizing under the stringency ions
described herein).
In an embodiment, the nucleic acid further comprises a nucleotide sequence encoding at least
one, two, or three CDRs from a heavy chain variable region of a polypeptide (e.g., an antibody
molecule or TCR molecule) bed herein, or a nucleotide sequence substantially homologous
thereto (e.g., a sequence at least about 85%, 90%, 95%, 99% or more identical thereto, and/or capable
of hybridizing under the stringency conditions described herein). In another embodiment, the nucleic
acid further comprises a nucleotide sequence encoding at least one, two, or three CDRs from a light
chain variable region of a polypeptide (e.g., an antibody le or TCR molecule) described herein,
or a nucleotide sequence substantially homologous thereto (e.g., a sequence at least about 85%, 90%,
95%, 99% or more identical thereto, and/or capable of hybridizing under the ency conditions
described herein). In yet another embodiment, the nucleic acid comprises a tide sequence
encoding at least one, two, three, four, five, or six CDRs from heavy and light chain variable regions
of a ptide (e.g., an antibody le or TCR le) described herein, or a nucleotide
sequence substantially homologous thereto (e.g., a sequence at least about 85%, 90%, 95%, 99% or
more identical thereto, and/or capable of hybridizing under the stringency conditions described
herein) .
In an embodiment, the nucleic acid comprises a portion of a nucleotide sequence described
herein. The portion may encode, for example, a variable region (e.g., VH or VL); one, two, or three
or more (e.g., four, five, or six) CDRs; or one, two, three, or four or more framework regions,
optionally, a constant region or an Fc region.
The c acids disclosed herein include deoxyribonucleotides or cleotides, or
analogs thereof. The polynucleotide may be either single-stranded or double-stranded, and if -
ed may be the coding strand or non-coding (antisense) strand. A polynucleotide may comprise
modified nucleotides, such as methylated tides and nucleotide analogs. The sequence of
nucleotides may be interrupted by non-nucleotide components. A polynucleotide may be further
modified after polymerization, such as by conjugation with a labeling component. The nucleic acid
may be a recombinant polynucleotide, or a cleotide of genomic, cDNA, semisynthetic, or
synthetic origin which either does not occur in nature or is linked to another polynucleotide in a non-
natural ement.
In some aspects, the ation features host cells and vectors containing the nucleic acids
described herein. The nucleic acids may be present in a single vector or separate vectors present in
the same host cell or separate host cell, as described in more detail below.
Vectors
The present disclosure features s that comprise nucleotide sequences encoding
polypeptides (e.g., binding polypeptides, e.g., antibody molecules or TCR molecules).
The vectors include, but are not limited to, a virus, plasmid, cosmid, lambda phage or a yeast
artificial some (YAC).
Numerous vector systems can be employed. For example, one class of vectors utilizes DNA
elements which are d from animal viruses such as, for example, bovine papilloma virus,
polyoma Virus, adenovirus, ia virus, baculovirus, retroviruses (Rous Sarcoma Virus, MMTV or
MOMLV) or SV4O virus. Another class of vectors utilizes RNA elements derived from RNA viruses
such as Semliki Forest Virus, Eastern Equine Encephalitis Virus and Flaviviruses.
Additionally, cells which have stably integrated the DNA into their chromosomes may be
selected by introducing one or more markers which allow for the selection of transfected host cells.
The marker may provide, for example, prototropy to an auxotrophic host, biocide resistance (e.g.,
antibiotics), or resistance to heavy metals such as copper, or the like. The able marker gene can
be either directly linked to the DNA sequences to be expressed, or introduced into the same cell by
sformation. Additional elements may also be needed for l synthesis of mRNA. These
elements may e splice signals, as well as transcriptional promoters, enhancers, and termination
signals.
Once the expression vector or DNA sequence containing the constructs has been prepared for
expression, the expression vectors may be transfected or introduced into an appropriate host cell.
Various techniques may be ed to achieve this, such as, for example, protoplast fusion, calcium
phosphate precipitation, oporation, retroviral transduction, viral ection, gene gun, lipid
based transfection or other conventional techniques. In the case of protoplast fusion, the cells are
grown in media and screened for the appropriate activity.
Methods and conditions for culturing the resulting transfected cells and for recovering the
polypeptide (e. g., antibody molecule) produced are known to those skilled in the art, and may be
varied or optimized depending upon the specific expression vector and mammalian host cell
employed, based upon the present description.
Cells
The present disclosure also provides host cells comprising a nucleic acid encoding a
polypeptide (e.g., an antibody le or TCR le) as described herein. The ptide (e.g.,
antibody molecule or TCR molecule) can be engineered in accordance with a method described
herein. For example, the host cells may se a nucleic acid molecule having a nucleotide
sequence of a polypeptide described herein (e.g., an antibody molecule or TCR molecule bed
herein), a sequence substantially homologous thereto (e.g., a sequence at least about 85%, 90%, 95%,
99% or more identical o, and/or capable of hybridizing under the stringency conditions
described ), or a portion of one of said nucleic acids.
In some embodiments, the host cells are genetically ered to comprise nucleic acids
encoding the polypeptide (e.g., antibody molecule or TCR molecule) described herein.
In certain embodiments, the host cells are genetically engineered by using an expression
cassette. The phrase “expression cassette,” refers to nucleotide sequences, which are capable of
affecting expression of a gene in hosts compatible with such sequences. Such cassettes may include a
promoter, an open reading frame with or without introns, and a termination signal. Additional factors
necessary or helpful in ing expression may also be used, such as, for example, an inducible
promoter.
The disclosure also provides host cells comprising the vectors described herein.
The cell can be, but is not d to, a eukaryotic cell, a bacterial cell, an insect cell, or a
human cell. Suitable eukaryotic cells include, but are not limited to, Vero cells, HeLa cells, COS cells,
CHO cells, HEK293 cells, BHK cells and MDCKII cells. Suitable insect cells include, but are not
limited to, Sf9 cells.
Uses of Polypeptides
The polypeptides (e.g., g polypeptides, e.g., antibody molecules or TCR molecule)
disclosed herein, as well as the pharmaceutical compositions sed herein, have in vitro, ex vivo,
and in viva therapeutic, prophylactic, and/or diagnostic utilities.
In an embodiment, the polypeptide (e.g., antibody molecule or TCR molecule) modulates
(e.g., s (e.g., ts, blocks, or neutralizes) or increases (e.g., activates, initiates, or es))
one or more biological activities of a target molecule (e. g., protein) or cell. For example, these
polypeptides (e.g., antibody les or TCR molecules) can be administered to cells in culture, in
vitra or ex viva, or to a subject, e.g., a human subject, e.g., in viva, to modulate one or more biological
activities of the target molecule or cell. Accordingly, in an aspect, the disclosure provides a method
of treating, preventing, or diagnosing a disorder, e. g., a er described herein, in a subject,
comprising administering to the t a polypeptide (e.g., an antibody molecule or TCR molecule)
described herein, such that the disorder is treated, prevented, or diagnosed. For example, the
disclosure provides a method comprising contacting the polypeptide (e.g., antibody le or TCR
molecule) described herein with cells in e, 6.3. in vitra or ex viva, or administering the
polypeptide (e.g., antibody molecule or TCR molecule) described herein to a subject, e.g., in viva, to
treat, prevent, or diagnose a disorder, e.g., a disorder associated with a target molecule or cell (e.g., a
disorder described ).
As used herein, the term “subject” is intended to include human and non-human animals. In
some embodiments, the t is a human t, e.g., a human patient having a disorder described
herein, or at risk of having a disorder described herein. The term uman animals” includes
mammals and non-mammals, such as non-human primates. In an embodiment, the subject is a
human. The methods and compositions described herein are suitable for treating human patients for a
disorder described herein. Patients having a disorder described herein include those who have
developed a disorder described herein but are (at least temporarily) asymptomatic, patients who have
exhibited a symptom of a disorder described herein, or patients having a disorder related to or
associated with a disorder described herein.
Methods of Treating or Preventing Disorders
The polypeptides (e.g., antibody molecules or TCR molecules) described herein can be used
to treat or prevent disorders or conditions. In an embodiment, the polypeptide has an optimal or
improved half-life, which can be desirable for treating or ting the disorder or condition. While
not wishing to be bound by , it is believed that in an embodiment, the ptide described
herein (e.g., the polypeptide having an optimal or improved half-life) can provide one or more
benefits over another polypeptide having the same or similar g affinity and/or icity (e.g., a
polypeptide that does not have, or has not been engineered to have, an l or improved half-life).
These benefits can include, but are not limited to, an increased therapeutic or preventive efficacy, a
d dosage regimen, or an improved pharmacokinetic ty. In an embodiment, the
polypeptide includes a mutated Fc region as described herein.
Exemplary disorders or conditions that can be treated or ted by the polypeptides
described herein include, but are not limited to, a cancer (e.g., a solid tumor or a hematologic cancer),
an infectious disease (e.g., a bacterial infection or a viral infection), an immune disorder (e.g., an
autoimmune er), an inflammatory disorder, a metabolic disorder (e.g., es), a
cardiovascular er, an organ transplant rejection.
Exemplary cancers that can be d or prevented by the polypeptides described herein
include, but are not limited to, acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML),
adrenocortical carcinoma, Kaposi sarcoma, an AIDS -related lymphoma, y central nervous
system (CNS) lymphoma, anal cancer, appendix cancer, astrocytoma, atypical teratoid/rhabdoid
tumor, basal cell carcinoma, bile duct cancer, bladder cancer, bone cancer (e.g., Ewing sarcoma or
osteosarcoma and malignant fibrous histiocytoma), brain tumor (e.g., astrocytomas, brain stem
glioma, central nervous system atypical teratoid/rhabdoid tumor, central nervous system embryonal
tumor, central nervous system germ cell tumor, craniopharyngioma, or ependymoma), breast cancer,
bronchial tumor, Burkitt lymphoma, carcinoid tumor (e.g., gastrointestinal carcinoid tumor), cardiac
(heart) tumor, embryonal tumor, germ cell tumor, lymphoma, cervical cancer, cholangiocarcinoma,
ma, chronic lymphocytic leukemia (CLL), chronic myelogenous ia (CML), chronic
myeloproliferative neoplasm, colon cancer, colorectal cancer, craniopharyngioma, ous T-cell
lymphoma, ductal carcinoma in situ (DCIS), endometrial cancer, ependymoma, esophageal cancer,
esthesioneuroblastoma, Ewing sarcoma, extracranial germ cell tumor, extragonadal germ cell tumor,
eye cancer (e.g., intraocular melanoma or retinoblastoma), ian tube cancer, fibrous histiocytoma
of bone, osteosarcoma, gallbladder cancer, gastric (stomach) cancer, gastrointestinal oid tumor,
gastrointestinal stromal tumors (GIST), germ cell tumor (e. g., central nervous system tumor,
extracranial tumor, extragonadal tumor, ovarian cancer, or ular cancer), gestational trophoblastic
disease, glioma, hairy cell leukemia, head and neck cancer, hepatocellular (liver) cancer, Hodgkin
lymphoma, hypopharyngeal cancer, cular melanoma, islet cell tumor, pancreatic neuroendocrine
tumor, Kaposi sarcoma, kidney cancer (e.g., renal cell cancer or Wilms tumor), Langerhans cell
histiocytosis (LCH), laryngeal cancer, leukemia (e.g., acute lymphoblastic leukemia (ALL), acute
myeloid leukemia (AML), chronic lymphocytic ia (CLL), chronic myelogenous leukemia
(CML), or hairy cell ia), lip and oral cavity cancer, liver cancer, lung cancer (e.g., all
cell lung cancer (NSCLC) or small cell lung cancer), ma (e.g., elated, Burkitt lymphoma,
ous T-cell lymphoma, Hodgkin lymphoma, non-Hodgkin lymphoma, or primary central
nervous system (CNS) lymphoma), Waldenstrom lobulinemia, male breast cancer, malignant
fibrous histiocytoma of bone and osteosarcoma, melanoma (e.g., intraocular (eye) melanoma), Merkel
cell carcinoma, mesothelioma, metastatic squamous neck cancer, midline tract carcinoma, mouth
cancer, le endocrine neoplasia syndrome, multiple myeloma/plasma cell neoplasm, mycosis
fungoides, myelodysplastic me, myelodysplastic/myeloproliferative neoplasm, chronic
myeloproliferative sm, nasal cavity and paranasal sinus cancer, nasopharyngeal cancer,
neuroblastoma, oral cancer, lip and oral cavity cancer, oropharyngeal cancer, osteosarcoma and
malignant fibrous histiocytoma of bone, n cancer (e.g., epithelial ovarian cancer or germ cell
ovarian tumor), pancreatic cancer, atic neuroendocrine tumors (islet cell tumors),
papillomatosis, paraganglioma, paranasal sinus and nasal cavity cancer, parathyroid cancer, penile
cancer, pharyngeal cancer, pheochromocytoma, pituitary tumor, pleuropulmonary blastoma,
neal cancer, prostate cancer, rectal cancer, retinoblastoma, rhabdomyosarcoma, salivary gland
cancer, sarcoma (e. g., Ewing sarcoma, Kaposi sarcoma, osteosarcoma, myosarcoma, soft tissue
sarcoma, or uterine sarcoma), Sézary syndrome, skin cancer (e.g., melanoma, Merkel cell carcinoma,
or nonmelanoma skin cancer), small intestine cancer, us cell carcinoma, testicular cancer,
throat cancer, thymoma and thymic carcinoma, thyroid cancer, transitional cell cancer of the renal
pelvis and ureter, urethral cancer, endometrial uterine cancer, vaginal cancer, vulvar , or a
metastatic lesion thereof.
ary infectious diseases that can be treated or prevented by the polypeptides described
herein include, but are not limited to, Acinetobacter infections, actinomycosis, African sleeping
sickness (African trypanosomiasis), AIDS (acquired immunodeficiency syndrome), amebiasis,
anaplasmosis, angiostrongyliasis, anisakiasis, anthrax, arcanobacterium haemolyticum infection,
argentine hemorrhagic fever, asis, aspergillosis, astrovirus infection, babesiosis, bacillus
cereus infection, bacterial pneumonia, bacterial vaginosis, bacteroides ion, balantidiasis,
bartonellosis, baylisascaris ion, bk virus infection, black , blastocystosis, mycosis,
bolivian hemorrhagic fever, botulism (and infant botulism), brazilian hemorrhagic fever, brucellosis,
bubonic plague, burkholderia infection, buruli ulcer, calicivirus ion (norovirus and sapovirus),
campylobacteriosis, candidiasis (moniliasis; thrush), ariasis, carrion’s disease, cat-scratch
disease, itis, chagas disease (american trypanosomiasis), chancroid, chickenpox, chikungunya,
chlamydia, chlamydophila pneumoniae ion (taiwan acute respiratory agent or twar), cholera,
chromoblastomycosis, chytridiomycosis, clonorchiasis, clostridium difficile colitis,
coccidioidomycosis, colorado tick fever (CTF), common cold (Acute viral rhinopharyngitis; Acute
coryza), Creutzfeldt-Jakob disease (CJD), Crimean-Congo hemorrhagic fever (CCHF),
cryptococcosis, cryptosporidiosis, cutaneous larva migrans (CLM), cyclosporiasis, cysticercosis,
cytomegalovirus infection, dengue fever, desmodesmus ion, dientamoebiasis, diphtheria,
diphyllobothriasis, dracunculiasis, ebola hemorrhagic fever, echinococcosis, hiosis,
enterobiasis rm infection), coccus infection, enterovirus infection, epidemic typhus,
erythema infectiosum (fifth disease), em subitum (sixth disease), fasciolasis, fasciolopsiasis,
fatal familial insomnia (FFI), filariasis, food poisoning by clostridium perfringens, free-living amebic
infection, fusobacterium ion, gas gangrene (clostridial myonecrosis), geotrichosis, gerstmann-
straussler-scheinker syndrome (GSS), giardiasis, glanders, gnathostomiasis, gonorrhea, granuloma
inguinale (donovanosis), Group A streptococcal infection, Group B streptococcal infection,
haemophilus influenzae infection, hand, foot and mouth disease (HFMD), Hantavirus ary
me (HPS), heartland virus disease, helicobacter pylori infection, hemolytic-uremic
syndrome (HUS), hemorrhagic fever with renal me (HFRS), hepatitis A, hepatitis B, hepatitis
C, hepatitis D, tis E, herpes x, histoplasmosis, hookworm infection, human
rus infection, human ewingii ehrlichiosis, human granulocytic anaplasmosis (HGA), human
metapneumovirus infection, Human monocytic ehrlichiosis, human papillomavirus (HPV) infection,
Human parainfluenza virus infection, Hymenolepiasis, Epstein-Barr Virus ious
Mononucleosis (Mono), influenza (flu), isosporiasis, kawasaki disease, keratitis, la
kingae infection, kuru, lassa fever, legionellosis nnaires' disease), legionellosis (pontiac fever),
leishmaniasis, leprosy, leptospirosis, listeriosis, lyme disease (lyme borreliosis), lymphatic
sis antiasis), Lymphocytic choriomeningitis, Malaria, Marng hemorrhagic
fever (MHF), Measles, Middle East respiratory syndrome (MERS), melioidosis (Whitmore's disease),
meningitis, meningococcal e, metagonimiasis, microsporidiosis, molluscum contagiosum (MC),
Monkeypox, Mumps, Murine typhus (Endemic typhus), Mycoplasma pneumonia, Mycetoma
biguation), Myiasis, Neonatal conjunctivitis (Ophthalmia orum), (New) Variant
Creutzfeldt-Jakob disease (vCJD, nvCJD), nocardiosis, onchocerciasis (River blindness),
opisthorchiasis, ccidioidomycosis (South an blastomycosis), paragonimiasis,
pasteurellosis, pediculosis capitis (head lice), losis corporis (body lice), pediculosis
pubis (pubic lice, crab lice), pelvic inflammatory disease (PID), pertussis (Whooping cough), plague,
pneumococcal infection, pneumocystis pneumonia (PCP), pneumonia, poliomyelitis,
prevotella ion, primary amoebic meningoencephalitis (PAM), progressive multifocal
leukoencephalopathy, psittacosis, Q fever, rabies, relapsing fever, respiratory syncytial
virus infection, rhinosporidiosis, rhinovirus infection, rickettsial infection, rickettsialpox, Rift Valley
fever (RVF), Rocky Mountain spotted fever (RMSF), rotavirus infection, rubella, salmonellosis,
SARS (Severe Acute Respiratory Syndrome), scabies, schistosomiasis, sepsis, shigellosis (Bacillary
dysentery), shingles (Herpes zoster), smallpox (Variola), sporotrichosis, staphylococcal food
poisoning, staphylococcal infection, strongyloidiasis, subacute sclerosing ephalitis, syphilis,
Taeniasis, Tetanus (Lockj aw), Tinea barbae (Barber's itch), Tinea capitis (Ringworm of the Scalp),
Tinea corporis (Ringworm of the Body), Tinea cruris (Jock itch), Tinea manum (Ringworm of the
Hand), Tinea nigra, Tinea pedis (Athlete’s foot), Tinea unguium (Onychomycosis), Tinea
versicolor (Pityriasis versicolor), Toxocariasis (Ocular Larva Migrans (OLM)), Toxocariasis (Visceral
Larva Migrans (VLM)), Trachoma, Toxoplasmosis, Trichinosis, Trichomoniasis,
Trichuriasis (Whipworm infection), Tuberculosis, Tularemia, Typhoid fever, Typhus fever,
Ureaplasma urealyticum infection, Valley fever, elan equine encephalitis, Venezuelan
hemorrhagic fever, Vibrio vulnificus ion, Vibrio parahaemolyticus enteritis, viral pneumonia,
West Nile Fever, white piedra (Tinea blanca), Yersinia pseudotuberculosis ion, yersiniosis,
yellow fever, Zika fever, or zygomycosis.
Exemplary immune disorders or conditions that can be treated or prevented by the
polypeptides described herein e, but are not limited to, Addison’s disease,
agammaglobulinemia, alopecia areata, amyloidosis, ankylosing litis, anti-GBM/anti-TBM
nephritis, antiphospholipid syndrome (APS), autoimmune hepatitis, autoimmune inner ear disease
(AIED), axonal & neuronal neuropathy (AMAN), Behcet’s disease, Bullous pemphigoid, Castleman
disease (CD), Celiac disease, Chagas disease, chronic inflammatory demyelinating polyneuropathy
(CIDP), chronic recurrent multifocal osteomyelitis (CRMO), Churg-Strauss, icial
pemphigoidfbenign mucosal pemphigoid, Cogan’s syndrome, Cold agglutinin disease, Congenital
heart block, Coxsackie myocarditis, CREST me, Crohn’ s disease, itis herpetiformis,
dermatomyositis, Devic’s disease (neuromyelitis ), Discoid lupus, Dressler’s syndrome,
endometriosis, eosinophilic esophagitis (EoE), eosinophilic tis, erythema nodosum, essential
mixed cryoglobulinemia, Evans syndrome, fibromyalgia, fibrosing alveolitis, giant cell arteritis
ral arteritis), giant cell myocarditis, Glomerulonephritis, Goodpasture’s syndrome,
Granulomatosis with Polyangiitis, Graves’ disease, in-Barre syndrome, oto’s thyroiditis,
hemolytic anemia, Henoch-Schonlein purpura (HSP), herpes gestationis or pemphigoid gestationis
(PG), hypogammalglobulinemia, IgA nephropathy, IgG4-related sclerosing disease, inclusion body
myositis (IBM), interstitial cystitis (IC), le arthritis, juvenile diabetes (Type 1 diabetes),
juvenile myositis (JM), Kawasaki disease, t-Eaton syndrome, leukocytoclastic vasculitis,
Lichen planus, Lichen sclerosus, Ligneous conjunctivitis, linear IgA disease (LAD), lupus, Lyme
disease chronic, Meniere’s e, microscopic polyangiitis (MPA), mixed connective tissue e
(MCTD), Mooren’s ulcer, Mucha-Habermann disease, multiple sclerosis (MS), enia gravis,
is, Narcolepsy, Neuromyelitis optica, neutropenia, ocular cicatricial pemphigoid, optic neuritis,
palindromic tism (PR), PANDAS (Pediatric mune Neuropsychiatric ers
Associated with Streptococcus), paraneoplastic cerebellar degeneration (PCD), smal nocturnal
hemoglobinuria (PNH), Parry g syndrome, Pars planitis (peripheral uveitis), Parsonnage-
Turner syndrome, Pemphigus, peripheral neuropathy, Perivenous encephalomyelitis, ious
anemia (PA), POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, monoclonal
gammopathy, skin changes), polyarteritis nodosa, polymyalgia rheumatica, polymyositis,
postmyocardial infarction syndrome, postpericardiotomy syndrome, primary biliary cirrhosis, primary
sing cholangitis, progesterone dermatitis, psoriasis, psoriatic arthritis, pure red cell aplasia
(PRCA), pyoderma gangrenosum, Raynaud’s phenomenon, Reactive Arthritis, Reflex sympathetic
dystrophy, Reiter’s syndrome, relapsing polychondritis, restless legs syndrome (RLS), retroperitoneal
fibrosis, rheumatic fever, rheumatoid tis (RA), sarcoidosis, Schmidt syndrome, tis,
scleroderma, Sj ogren’s syndrome, sperm & testicular autoimmunity, Stiff person syndrome (SPS),
subacute bacterial endocarditis (SBE), Susac’s syndrome, sympathetic ophthalmia (SO), Takayasu’s
arteritis, temporal arteritis/Giant cell arteritis, thrombocytopenic purpura (TTP), Tolosa-Hunt
syndrome (THS), transverse myelitis, type 1 diabetes, ulcerative colitis (UC), undifferentiated
connective tissue disease (UCTD), uveitis, vasculitis, vitiligo, or Wegener’s granulomatosis
(Granulomatosis with Polyangiitis (GPA)).
The ptides (e.g., dy molecules or TCR molecules) described herein are typically
stered at a frequency that keeps a therapeutically effective level of polypeptides in the patient’s
system until the patient recovers. For example, the polypeptides may be administered at a frequency
that achieves a serum tration sufficient for at least about 1, 2, 5, 10, 20, 30, or 40 polypeptides
to bind each target le or cell. In an embodiment, the polypeptides are administered every 1, 2,
3, 4, 5,6, or 7 days, every 1,2,3, 4, 5, or 6 weeks, or every 1, 2, 3, 4,5, or 6 months.
Methods of administering various polypeptides (e.g., dy molecules or TCR molecules)
are known in the art and are described below. Suitable dosages of the ptides used will depend
on the age and weight of the subject and the particular drug used.
The polypeptides can be used by themselves or conjugated to a second agent, e.g., an bacterial
agent, toxin, or protein, e.g., a second polypeptide. This method includes: administering the
polypeptide, alone or conjugated to a second agent, to a subject requiring such treatment. The
polypeptides can be used to deliver a variety of therapeutic agents, e.g., a toxin, or es thereof.
Combination Therapies
The polypeptides (e.g., antibody molecules or TCR molecules) can be used in combination
with other therapies. For example, the combination therapy can e a polypeptide co-formulated
with, and/or co-administered with, one or more additional therapeutic agents, e. 3., one or more
additional therapeutic agents described herein. In other embodiments, the polypeptides are
administered in combination with other therapeutic treatment modalities, e.g., other therapeutic
treatment modalities described . Such combination therapies may advantageously utilize lower
dosages of the administered therapeutic agents, thus avoiding possible toxicities or complications
associated with the various monotherapies.
Administered “in combination”, as used herein, means that two (or more) different treatments
are delivered to the subject before, or during the course of the subject's affliction with a disorder. In
an embodiment, two or more ents are delivered lactically, e.g., before the subject has the
disorder or is diagnosed with the er. In another ment, the two or more treatments are
delivered after the subject has developed or diagnosed with the disorder. In some ments, the
delivery of one treatment is still occurring when the delivery of the second begins, so that there is
overlap. This is mes referred to herein as "simultaneous" or "concurrent ry." In other
embodiments, the delivery of one treatment ends before the delivery of the other treatment begins. In
some embodiments of either case, the treatment is more effective because of combined administration.
For example, the second treatment is more effective, e.g., an equivalent effect is seen with less of the
second treatment, or the second treatment reduces symptoms to a greater extent, than would be seen if
the second treatment were administered in the absence of the first treatment, or the analogous
situation is seen with the first treatment. In some embodiments, delivery is such that the reduction in
a symptom, or other parameter related to the disorder is greater than what would be observed with one
treatment delivered in the absence of the other. The effect of the two treatments can be partially
ve, wholly additive, or greater than additive. The delivery can be such that an effect of the first
treatment delivered is still detectable when the second is delivered.
In an embodiment, the polypeptide is administered in combination with a second therapy
(e. g., an additional agent) to treat or prevent a er described herein. In an embodiment, the
additional agent is a second ptide (e.g., antibody molecule), e.g., a polypeptide (e.g., an
antibody molecule) different from a first ptide (e.g., antibody molecule). Exemplary
polypeptides (e.g., antibody molecules) that can be used in combination include, but are not limited
to, any combination of the ptides (e.g., antibody molecules) described herein. In another
embodiment, the additional agent is other than a polypeptide (e.g., antibody molecule). For example,
the additional agent can be a small molecule or a nucleic acid molecule. In yet another embodiment,
the second therapy is chosen from a surgery, a radiation therapy, a cell therapy (e.g., a stem cell
therapy), or an organ or tissue transplantation.
In an embodiment, the second therapy comprises a therapy chosen from one or more of: an
androgen replacement therapy, an antihormone y, an antiserum therapy, an autologous immune
enhancement therapy, a biotherapy, a blood irradiation therapy, a brachytherapy, a cardiac
resynchronization therapy, a cell therapy, a cell transfer therapy, a chelation y, a herapy,
a chrysotherapy, a cobalt therapy, a cold compression therapy, a cryotherapy, an electroconvulsive
therapy, an electromagnetic y, an electron therapy, an electrotherapy, an enzyme replacement
therapy, an epigenetic therapy, an estrogen replacement therapy, an extracorporeal shockwave
therapy, a fast neutron therapy, a e therapy, a gene therapy, a heat therapy, a helminthic
therapy, a hormone therapy, a hormone replacement therapy, a host modulatory therapy, a hyperbaric
oxygen therapy, a hyperthermia y, an immunosuppressive therapy, an immunotherapy, an
intraoperative electron ion therapy, an intraoperative radiation therapy, an inversion therapy, a
laser therapy, a light y, a lithium therapy, a low level laser therapy, a magnet therapy, a
magnetic resonance therapy, a medical gas y, a medical nutrition therapy, a molecular
chaperone therapy, a molecular y, a monoclonal antibody therapy, a negative air ionization
therapy, a n capture therapy, a neutron therapy, an oral rehydration therapy, an osmotherapy, an
oxygen therapy, an ozone therapy, a palliative therapy, a particle therapy, a phage therapy, a
phonemic ogical hypochromium therapy, a photodynamic therapy, a herapy, a
photothermal therapy, a physical therapy, a prolotherapy, a protein therapy, a proton therapy, a pulsed
electromagnetic field therapy, a PUVA y, a radiation therapy, a rehydration therapy, a
respiratory therapy, salvage therapy, a serotherapy, a stem cell therapy, a stereotactic radiation
therapy, a targeted therapy, a therapy, a TK cell therapy, a tolerogenic therapy, a transdermal
continuous oxygen therapy, an ultraviolet light therapy, or a virotherapy.
Exemplary therapies that can be used in combination with a polypeptide or composition
described herein to treat or prevent other disorders are also described in the section of “Methods of
Treating or Preventing Disorders” herein.
Methods of Diagnosis
In some aspects, the present disclosure provides a diagnostic method for detecting the
presence of a target molecule (e.g., a n) or cell in vitro (e.g., in a biological sample, such as a
biopsy or body fluid (e.g., blood, urine, or cerebrospinal fluid) sample) or in viva (e.g., in vivo
imaging in a subject). The method includes: (i) contacting the sample with a polypeptide described
herein (e.g., an antibody molecule described herein), or administering to the subject, the ptide
(6.3., dy molecule); (optionally) (ii) contacting a reference sample, e.g., a control sample (e.g., a
control biological sample, such as a biopsy or body fluid (e.g., blood, urine, or cerebrospinal fluid)
) or a control subject with a polypeptide described herein (e.g., an antibody molecule described
herein); and (iii) detecting formation of a complex between the polypeptide (e. g., antibody molecule)
and the target molecule or cell in the sample or subject, or the control sample or subject, wherein a
change, e.g., a statistically significant change, in the formation of the complex in the sample or subject
relative to the control sample or subject is indicative of the ce of the target molecule or cell in
the sample. The polypeptide (e.g., antibody molecule) can be ly or indirectly labeled with a
able substance to facilitate detection of the bound or unbound polypeptide (e.g., antibody
molecule). le detectable substances include various enzymes, prosthetic groups, fluorescent
materials, luminescent materials and radioactive materials, as described herein.
The term “sample,” as it refers to samples used for detecting bacteria includes, but is not
limited to, cells, cell lysates, proteins or membrane ts of cells, body fluids such as blood, urine,
or CSF, or tissue samples such as biopsies.
Complex ion n the polypeptide (e.g., antibody molecule), and the target
molecule or cell, can be detected by measuring or visualizing either the polypeptide (e.g., antibody
molecule) bound to the target molecule or cell, or unbound polypeptide (e.g., antibody molecule).
Any suitable detection assays can be used, and conventional detection assays include an -
linked imrnunosorbent assay (ELISA), a radioimmunoassay (RIA), a FACS assay, a BIACORE assay,
or tissue immunohistochemistry. Alternative to labeling the polypeptide, the presence of the target
molecule or cell can be d in a sample by a ition immunoassay utilizing standards labeled
with a able substance and an unlabeled polypeptide. In this assay, the biological sample, the
labeled standards and the polypeptide are ed and the amount of labeled standard bound to the
unlabeled binding molecule is determined. The amount of the target molecule or cell in the sample is
inversely proportional to the amount of labeled standard bound to the polypeptide (e.g., antibody
molecule).
The polypeptides (e.g., antibody molecules) described herein can be used to diagnose
disorders that can be treated or prevented by the polypeptides described herein. The detection or
diagnostic methods described herein can be used in combination with other methods described herein
to treat or prevent disorders bed herein.
Additional embodiments are described in the numbered paragraphs below.
1. A method of making a nucleic acid sequence sing a sequence that encodes a heavy
chain element (HC element) of an antibody heavy chain variable region (HCVR) and a light chain
element (LC element) of an antibody light chain variable region (LCVR), and n the HCVR and
LCVR are matched, the method comprising:
a) acquiring an isolated production reaction site, e.g., a production micro-chamber,
comprising:
i) a heavy chain (HC) strand, wherein the HC strand is a strand of a heavy chain
-stranded cDNA (HC ds cDNA) comprising a segment that encodes an HC element of
the HCVR from a cell, e.g., a heavy chain variable region sequence (HCVRS); and
ii) a light chain (LC) strand, wherein the LC strand is a strand of a light chain double-
stranded cDNA (LC ds cDNA) comprising a segment that encodes an LC element of the
LCVR from the cell, e. 3., a light chain variable region sequence (LCVRS), and
b) covalent linking, e.g., ligation, of an HC strand to an LC strand,
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding an LCVR or an HCVR from a cell other than the cell,
thereby making a c acid sequence comprising a sequence that encodes an HC element
of an HCVR and a LC element of an LCVR, wherein the HCVR and LCVR are matched.
2. The method of paragraph 1, wherein the HC element comprises, or consists of, an
HCVRS, or a functional fragment f (e.g., an antigen binding fragment thereof).
3. The method of aph 1 or 2, n the LC element comprises, or consists of, an
LCVRS, or a functional fragment thereof (e.g., an antigen g fragment thereof).
4. The method of any of paragraphs 1-3, wherein the nucleic acid ce is configured
such that, when expressed, the HCVRS and the LCVRS form a functional antigen binding molecule,
e.g., an scFv.
. The method of paragraph 4, wherein the n binding molecule, e.g., an scFv, is
functional in vitro, ex vivo, or in viva, e.g., as ined by a method or assay bed herein.
6. The method of any of aphs 1-5, wherein acquiring an isolated production reaction
site, e.g., a production micro-chamber, comprises:
a) acquiring a capture substrate bound to:
(i) a first double-stranded cDNA (ds cDNA) comprising a strand that is
complementary to a first mRNA that encodes an HCVR from a cell; and
(ii) a second ds cDNA sing a strand complementary to a second mRNA
encoding an LCVR from the cell (the cDNA loaded capture substrate), and
b) ining the isolated production reaction site, e.g., the production micro-chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce:
a plurality of HC ds cDNAs comprising a segment that s an HC element of the HCVR
from the cell, e.g., an HCVRS; and
a plurality of LC ds cDNAs comprising a t that encodes an LC element of the LCVR
from the cell, e.g., an LCVRS.
7. The method of paragraph 6, wherein the HC ds cDNA is identical, or substantially
identical, to the first ds cDNA.
8. The method of paragraph 6 or 7, wherein the LC ds cDNA is identical, or substantially
cal, to the second ds cDNA.
9. The method of any of paragraphs 6-8, wherein the capture substrate comprises a bead, e. g.,
a ic bead.
10. The method of any of paragraphs 6-9, wherein the capture substrate comprises a moiety
(e.g., an oligonucleotide) which binds to cDNA, e.g., (i) a moiety which binds to the HC strand; (ii) a
moiety which binds to the LC strand; or (iii) both (i) and (ii).
11. The method of any of paragraphs 6-10, wherein the first mRNA and the second mRNA
are disposed on an mRNA loaded capture substrate.
12. The method of any of paragraphs 6-11, wherein the isolated production reaction site, e.g.,
the production micro-chamber, comprises:
a reagent mixture suitable for producing, from the first and second mRNAs (e. g., after the
first and second mRNAs are released from the mRNA loaded capture substrate into a solution), a first
ds cDNA comprising a segment that s an HC t of the HCVR of the cell, e. g., an
HCVRS, and a second ds cDNA comprising a segment that s an LC element of the LCVR of
the cell, e.g., an LCVRS.
13. The method of any of paragraphs 6-12, n the isolated production reaction site, e.g.,
production micro-chamber, comprises primers that mediate the production of the first ds cDNA.
14. The method of any of paragraphs 6-13, wherein the isolated production reaction site, e.g.,
production micro-chamber, comprises primers that mediate the production of the second ds cDNA.
. The method of any of paragraphs 6-14, wherein a cDNA strand that is complementary to
a first mRNA that encodes an HCVR from a cell is made by reverse transcription of the first mRNA.
16. The method of any of paragraphs 6-15, wherein a cDNA strand that is complementary to
a second mRNA that encodes an LCVR from a cell is made by reverse ription of the second
mRNA.
17. The method of paragraph 15 or 16, wherein the reverse transcription takes place in the
isolated production reaction site, e.g., a production-micro chamber.
18. The method of paragraph 15 or 16, wherein the reverse transcription takes place in an
isolated cell reaction site, e.g., a cell isolation micro-chamber.
19. The method of paragraph 15 or 16, wherein the e transcription takes place outside
the isolated production reaction site, e.g., a production micro-chamber, or outside an isolated cell
reaction site, e. g., a cell ion micro-chamber.
. The method of paragraph 15 or 16, wherein the reverse transcription takes place outside
the isolated production reaction site, e. g., a production-micro r, and outside an isolated cell
reaction site, e.g., a cell isolation micro-chamber.
21. The method of paragraph 15 or 16, wherein the reverse transcription takes place outside
an isolated reaction site, e.g., outside a chamber.
22. The method of any of aphs 6-21, wherein the amplification comprises 20 or fewer
, e.g., 15 or fewer, 14 or fewer, 13 or fewer, 12 or fewer, 11 or fewer, 10 or fewer, 9 or fewer, 8
or fewer, 7 or fewer, 6 or fewer, or 5 or fewer cycles.
23. The method of any of paragraphs 6-22, wherein the e transcription and/or
ication uses one or more primers, e. g., comprising a sequence specific for an HCVRS and/or an
LCVRS.
24. The method of any of paragraphs 6-23, wherein the amplification comprises using two or
more primers that mediate the production of the HC ds cDNA, wherein at least one primer comprises
a nucleotide modification, and wherein at least one primer does not comprise a nucleotide
modification.
. The method of any of aphs 6-24, wherein the amplification comprises using two or
more primers that mediate the tion of the LC ds cDNA, wherein at least one primer ses
a nucleotide modification, and wherein at least one primer does not comprise a nucleotide
modification.
26. The method of paragraph 25, wherein at least one primer comprises a nucleotide
modification, e.g., which reduces, e.g., inhibits, DNA synthesis, e.g., by a DNA polymerase.
27. The method of paragraph 25 or 26, wherein at least one primer does not comprise a
nucleotide modification, e.g., which reduces, e.g., inhibits, DNA synthesis, e.g., by a DNA
polymerase.
28. The method of paragraph 26 or 27, wherein the nucleotide cation inhibits a DNA
polymerase from extending the DNA.
29. The method of any of paragraphs 26-28, wherein the nucleotide modification is an
insertion of a spacer to the , e.g., between two adjacent nucleotides in the primer.
. The method of paragraph 29, wherein the spacer is a flexible spacer, a carbon spacer
(e.g., -(CH2)n-, wherein n=3, 4, 5, or more), two or more (e.g., three, four, five, or more) abasic
nucleotides or a PEG spacer.
31. The method of any of paragraphs 26-28, wherein the nucleotide modification is 2’-O-
methyl, 2’-OH, 2’-NH2, or uracil, e.g., to a ribose.
32. The method of any of paragraphs 26-31, wherein the nucleotide modification is located
internally or at the 3’ end of the primer.
33. The method of any of paragraphs 23-32, wherein at least one primer comprises (i) a first
; (ii) a second member; and optionally (iii) a nucleotide modification described herein, e.g.,
located between (i) and (ii).
34. The method of paragraph 33, wherein the first member is e of annealing with the
second member in the same primer or a different primer, e.g., forming a hairpin structure (via
intramolecular hybridization) or a -stranded structure (via intermolecular ization)
comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, ll, 12, 13, 14, 15, 16, 17, 18, 19, 20, more
basepairs.
. The method of paragraph 33 or 34, wherein the first member comprises a sequence that is
mentary to the sequence of an oligonucleotide attached to the capture substrate.
36. The method of any of paragraphs 33-35, wherein the second member comprises (e.g.,
from 5’ to 3’) one, two, or all of: (i) a sequence that is complementary to at least a portion of the first
member; (ii) a universal priming sequence (e.g., for PCR amplification or next-generation
sequencing); and (iii) a sequence complementary to a target sequence, e.g., an HCVRS and/or a
LCVRS, ally, wherein the second member ses a sequence for homologous
recombination (e.g., in a yeast or mammalian cell).
37. The method of any of paragraphs 23-36, n at least one primer comprises a
sequence encoding at least a portion of a linker sequence, or a complementary sequence thereof.
38. The method of paragraph 37, wherein the primer that comprises a sequence encoding at
least a portion of a linker sequence, or a complementary sequence thereof, is orylated, e.g., 5’
phosphorylated.
39. The method of paragraph 37 or 38, wherein the linker sequence comprises, or consists of,
(Gly-Gly-Gly-Gly-Ser)n, where n=1, 2, 3, 4, 5, or more.
40. The method of any of paragraphs 1-39, n the HC ds cDNA comprises a 5’
overhang, e.g., a 5’ overhang that is capable of hybridizing to an oligonucleotide attached to a capture
substrate.
41. The method of any of paragraphs 1-40, wherein the HC ds cDNA comprises a blunt end,
e.g., a blunt end comprising a 5’ phosphate.
42. The method of any of paragraphs 1-41, n the LC ds cDNA comprises a 5’
overhang, e.g., a 5’ overhang that is capable of hybridizing to an ucleotide attached to a capture
substrate.
43. The method of any of paragraphs 1-42, wherein the LC ds cDNA comprises a blunt end,
e.g., a blunt end comprising a 5’ phosphate.
44. The method of any of paragraphs 1-43, wherein the HC ds cDNA and the LC ds cDNA
comprise sticky ends, e.g., both have 5’ overhangs.
45. The method of any of paragraphs 1-44, wherein the HC strand and the LC strand are
covalently linked, e.g., d, to produce a single stranded nucleic acid sequence, wherein the HC
and LC strands are both sense strands or both antisense strands.
46. The method of any of paragraphs 1-44, wherein a denatured HC strand of the HC ds
cDNA to a denatured LC strand of the LC ds cDNA are covalently linked, e.g., ligated, wherein the
HC and LC strands are both sense strands or both antisense strands.
47. The method of any of paragraphs 1-44, wherein the HC strand is present in the HC ds
cDNA and the LC strand is present in the LC ds cDNA, and wherein the HC ds cDNA and the LC ds
cDNA are covalently linked, e.g., ligated, e.g., to produce a double stranded nucleic acid sequence.
48. The method of any of paragraphs 1-47, wherein the covalent g, e.g., ligation, occurs
in the isolated production reaction site.
49. The method of paragraph 48, wherein the isolated production reaction site, e.g., a
production micro-chamber, ses a reagent that is capable of covalently linking, e.g., ligating, the
HC and LC strands or the HC and LC ds cDNAs.
50. The method of aph 48 or 49, wherein the isolated tion reaction site, e.g., a
production micro-chamber comprises an enzyme that covalently couples the HC and LC s or the
HC and LC ds cDNAs.
51. The method of any of aphs 1-47, wherein the covalent linking, e.g., ligation, occurs
in a site different from the isolated production reaction site, e.g., occurs in an isolated linkage reaction
site, e.g., a linkage micro-chamber.
52. The method of paragraph 51, wherein the HC strand and the LC strand are transferred
from the isolated production site to the isolated linkage reaction site, e.g., a e micro-chamber,
and the covalent linking occurs in the isolated linkage reaction site, e.g., a e micro-chamber.
53. The method of paragraph 51 or 52, n the isolated linkage reaction site, e.g., a
linkage micro-chamber, comprises a reagent that is capable of covalently linking, e.g., ligating, the
HC and LC strands or the HC and LC ds cDNAs.
54. The method of any of paragraphs 51-53, wherein the isolated linkage reaction site, e. g., a
linkage micro-chamber, ses an enzyme that covalently couples the HC and LC strands or the
HC and LC ds cDNAs.
55. The method of aph 50 or 54, wherein the enzyme is a ligase, e.g., a thermal stable
ligase.
56. The method of any of paragraphs 51-55, wherein the covalent linking, e.g., on,
comprises:
(a) heating the isolated linkage on site, e.g., the linkage micro-chamber, under
conditions (e.g., at 95°C) that allow denaturation of the HC strand and the LC strand;
(b) cooling the isolated linkage reaction site, e.g., the linkage micro-chamber, under
conditions (e.g., at 50-65°C) that allow hybridization of the splint oligonucleotide to the HC strand
and the LC strand;
(c) maintaining the isolated linkage reaction site, e.g., the linkage micro-chamber, under
conditions (e.g., at 45-65°C) that allow ligation of the HC strand and the LC strand (e.g., formation of
phosphodiester bond between the HC strand and the LC strand); and
(d) repeating steps (a), (b), and (c) sequentially for 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, or
more cycles.
57. The method of any of paragraphs 1-56, wherein the HC strand and the LC strand are
covalently linked, e.g., ligated, in the ce of a splint oligonucleotide.
58. The method of paragraph 57, wherein the splint oligonucleotide is hybridized to a
sequence comprising the junction of the HC strand and the DC , or a sequence complementary
thereof, and forms a duplexed region at the site of ligation.
59. The method of paragraph 57 or 58, wherein the splint oligonucleotide comprises a
modification (e.g., an NH2 group) that inhibits DNA synthesis, e.g., by a DNA polymerase.
60. The method of paragraph 59, wherein the modification is at the 3’ end of the splint
ucleotide.
61. The method of any of paragraphs 1-60, n a strand complimentary to the covalently
linked, e.g., ligated, HC and LC strands is produced by ication.
62. The method of any of aphs 1-61, further comprising, prior to acquiring the isolated
production reaction site, e.g., a production micro-chamber, acquiring an mRNA loaded capture
substrate.
63. The method of paragraph 62, n acquiring the mRNA loaded capture substrate
comprising:
a) acquiring an ed cell reaction site, e.g., a cell isolation micro-chamber, comprising:
i) a cell; and
ii) a capture substrate capable of binding a first mRNA encoding an HCVR from the
cell and a second mRNA ng an LCVR from the cell; and
b) maintaining the isolated cell reaction site, e.g., the cell isolation chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a
nucleic acid ng an HCVR or an LCVR from a cell other than the cell.
64. The method of paragraph 63, wherein the isolated cell on site, e. g., cell isolation
micro-chamber, comprises a lysing reagent, e.g., a detergent.
65. The method of paragraph 63 or 64, wherein the cell is lysed by heat or an enzyme.
66. The method of any of paragraphs 63-65, wherein the capture substrate comprises a
moiety (e.g., an oligonucleotide) which binds mRNA, e.g., an oligo(dT).
67. The method of any of paragraphs 62-66, further comprising releasing the mRNA loaded
capture substrate from the isolated cell reaction site, e.g., the cell isolation micro-chamber.
68. The method of paragraph 67, n the releasing step is performed in the presence of a
poly(dA) or poly(dT) oligonucleotide, e.g., to reduce cross-binding of non-captured mRNA.
69. The method of paragraph 62-68, wherein the mRNA loaded capture substrate is
transferred from the isolated cell reaction site, e.g., the cell ion micro-chamber, to the isolated
production reaction site, e.g., the production micro-chamber.
70. The method of any of paragraphs 1-69, comprising releasing the c acid sequence
from the production micro-chamber.
71. The method of any of paragraphs 1-70, further comprising amplifying the nucleic acid
sequence.
72. The method of any of paragraphs 1-71, comprising sequencing all or a portion of the
c acid sequence.
73. The method of any of paragraphs 1-72, comprising inserting all or a portion of c
acid sequence into a vector.
74. The method of aph 73, wherein the vector supplies an additional HC element or LC
element not included in the nucleic acid sequence.
75. The method of paragraph 73 or 74, n the vector supplies an HC CDRl, an HC
CDRZ, or both.
76. The method of any of paragraphs 73-75, comprising expressing the .
77. The method of any of paragraphs 1-76, comprising sing the c acid sequence
to produce a polypeptide comprising a segment that encodes an HC element of the HCVR, e.g., an
HCVRS, and a segment that encodes an LC element of the LCVR, e.g., an LCVRS.
78. The method of paragraph 77, wherein the LC element is N-terminal to the HC element in
the ptide.
79. The method of paragraph 77, wherein the HC element is C-terminal to the LC element in
the polypeptide.
80. The method of any of paragraphs 77-79, further comprising contacting the polypeptide
with an antigen.
81. The method of any of aphs 77-80, further comprising determining if the
polypeptide binds the antigen.
82. The method of any of paragraphs 1-81, wherein the cell is an immune cell, e.g., a B cell
or T cell, e. g., a human B cell or T cell.
83. The method of any of paragraphs 1-82, wherein the cell is a mammalian cell or an avian
cell.
84. A method of making a nucleic acid sequence comprising a sequence that s a heavy
chain element (HC element) of an antibody heavy chain variable region (HCVR) and a light chain
element (LC element) of an antibody light chain variable region (LCVR), and wherein the HCVR and
LCVR are matched, comprising:
a) acquiring an isolated cell reaction site, e.g., a cell isolation micro-chamber, comprising:
i) a cell; and
ii) a capture substrate capable of binding a first mRNA encoding an HCVR from the
cell and a second mRNA ng an LCVR from the cell;
b) maintaining isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the e substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture ate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a
nucleic acid encoding an HCVR or an LCVR from a cell other than the cell;
c) contacting the mRNA loaded capture substrate with a reaction mixture, e.g., a reaction
mixture comprising reverse transcriptase, that uses the loaded mRNA as a template to make cDNA
(this can occur, e.g., in the isolated cell on site, in the isolated production reaction site, or in
neither, e.g., not in an ed reaction site);
d) acquiring an isolated production reaction site, e.g., a production micro-chamber,
comprising:
i) a heavy chain (HC) strand from step b), wherein the HC strand is a strand of a
heavy chain double-stranded cDNA (HC ds cDNA) comprising a segment that encodes an HC
element of the HCVR from the cell, e.g., a heavy chain variable region sequence (HCVRS);
ii) a light chain (LC) strand from step b), wherein the LC strand is a strand of a light
chain -stranded cDNA (LC ds cDNA) comprising a segment that encodes an LC
element of the LCVR from the cell, e.g., a light chain variable region sequence (LCVRS); and
e) covalent linking, e.g., on, of an HC strand to an LC strand,
wherein the isolated production on site, e.g., a production micro-chamber, does not
include a nucleic acid encoding an LCVR or an HCVR from a cell other than the cell.
85. A method of making a nucleic acid sequence comprising a sequence that encodes a heavy
chain element (HC t) of an antibody heavy chain le region (HCVR) and a light chain
element (LC element) of an antibody light chain variable region , and n the HCVR and
LCVR are matched, comprising:
a) acquiring an isolated cell reaction site, e.g., a cell isolation micro-chamber, comprising:
i) a cell; and
ii) a capture substrate capable of binding a first mRNA encoding an HCVR from the
cell and a second mRNA encoding an LCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell isolation chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a
nucleic acid encoding an HCVR or an LCVR from a cell other than the cell;
c) acquiring an isolated production reaction site, e.g., a tion micro-chamber, comprises:
contacting the mRNA loaded capture substrate with a reaction mixture, e.g., a reaction mixture
comprising reverse transcriptase, that uses the loaded mRNA as a template, to produce:
a first double-stranded cDNA (ds cDNA) comprising a strand that is mentary to a first
mRNA that encodes an HCVR from a cell; and
a second ds cDNA comprising a strand complementary to a second mRNA encoding an
LCVR from the cell (the cDNA loaded e substrate);
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding an LCVR or an HCVR from a cell other than the cell.
d) ining the isolated production reaction site, e.g., the production micro-chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce:
a plurality of HC ds cDNAs comprising a segment that encodes an HC element of the HCVR
from the cell, e.g., an HCVRS; and
a plurality of LC ds cDNAs comprising a t that encodes an LC element of the LCVR
from the cell, e. g., an LCVRS;
e) acquiring an isolated linkage reaction site, e.g., a linkage micro-chamber, comprising:
covalent linking, e.g., ligation, of a strand of the HC ds cDNA (HC strand) to a strand of the LC ds
cDNA (LC strand), wherein the HC and LC strands are both sense strands or nse strands; and
f) amplifying the covalently linked, e.g., ligated, HC and LC strands.
86. A method of making a library comprising a ity of unique members, the method
comprising:
making the plurality of members, wherein each of the members comprises a sequence that
encodes a heavy chain t (HC element) of a heavy chain variable region (HCVR) and a light
chain element (LC element) of a light chain variable region , and wherein the HCVR and
LCVR are matched, made by a method of any of paragraphs 1-85,
wherein each unique nucleic acid ce of the plurality comprises an HC element and an
LC element from a different unique cell,
thereby making a library comprising a plurality of unique members.
87. The method of paragraph 86, wherein the ity of unique members ses at least
104, 105, 106, 107, 108, or 109 unique members.
88. The method of paragraph 86 or 87, wherein the plurality of unique members comprises
104 to 109, 104 to 108, 104 to 107, 104 to 106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109,
105 to 108, 106 to 107, 104 to 105, 105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique s.
89. The method of any of paragraphs 86-88, wherein at least 80%, 85 %, 90%, 95%, 96%,
97%, 98%, 99%, or 100%, of the s in the library are unique members (which encode matched
HC element and LC element sequences).
90. The method of any of paragraphs 86-89, wherein less than 20%, 15%, 10%, 5%, 4%, 3%,
2%, or 1%, of the members in the library are unique members (which encode matched HC element
and LC element sequences).
91. A library comprising:
a plurality of unique members,
wherein,
i) each unique member of the plurality comprises a segment that encodes an HC element, e.g.,
an HCVRS, and a segment that encodes an LC element, e.g., an LCVRS, wherein the HC element and
the LC element in each unique member is matched;
ii) each unique member of the plurality comprises a segment that encodes an HC element,
e.g., an HCVRS, and a segment that encodes an LC element, e.g., an LCVRS, from a different unique
cell; and
iii) the library ses one or more (e.g., two, three, four, or all) of the following
properties:
a) the library is made by a method of any of paragraphs 1-85;
b) the plurality of unique members comprises at least 104, 105, 106, 107, 108, or 109
unique nucleic acid sequences;
c) the plurality of unique members comprises 104 to 109, 104 to 108, 104 to 107, 104 to
106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 108, 106 to 107, 104 to 105,
105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique members;
(1) at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the members in
the y are unique members (which encode matched HC element and LC element
ces); or
e) less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the members in the library
are unique members (which encode matched HC element and LC element sequences).
92. The library of aph 91, wherein each unique member of the plurality is configured
such that, when expressed, the HC element, e.g., the HCVRS, and the LC element, e. g., the LCVRS,
form a functional antigen binding molecule, e.g., an scFV.
93. The y of any of paragraphs 91-92, wherein the library is a display library.
94. The library of any of paragraphs 91-93, wherein each of the members of the plurality
r s a polypeptide that s in display of the member on the e of a display entity.
95. The library of any of paragraphs 91-94, wherein the library is a phage display library.
96. The library of any of paragraphs 91-94, wherein the library is a yeast display library.
97. The y of any of paragraphs 91-94, wherein the y is a mammalian y
library.
98. A method of making a binding polypeptide, the method comprising:
a) acquiring a y of any of paragraphs 91-97; and
b) expressing a polypeptide d by a unique nucleic acid of the library.
99. The method of paragraph 98, further comprising contacting the polypeptide with an
antigen.
100. The method of paragraph 98 or 99, further comprising retrieving the nucleic acid that
encodes a polypeptide that binds the antigen.
101. A method of making a nucleic acid sequence comprising a sequence that encodes an 0:
chain element (AC element) of a TCR 0t chain variable region (ACVR) and a [3 chain element (BC
element) of a TCR [3 chain le region (BCVR), and wherein the ACVR and BCVR are d,
the method sing:
a) ing an isolated production reaction site, e.g., a production micro-chamber,
comprising:
i) an a chain (AC) , wherein the AC strand is a strand of an 0. chain -
stranded cDNA (AC ds cDNA) comprising a segment that encodes an AC element of the
ACVR from a cell, e.g., an a chain le region sequence (ACVRS); and
ii) a [5 chain (BC) strand, wherein the BC strand is a strand of a B chain double-
stranded cDNA (BC ds cDNA) comprising a segment that encodes a BC element of the
BCVR from the cell, e.g., a [3 chain variable region ce (BCVRS), and
b) covalent linking, e.g., ligation, of an AC strand to a BC strand,
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding a BCVR or an ACVR from a cell other than the cell,
thereby making a nucleic acid sequence comprising a sequence that encodes an AC element
of an ACVR and a BC element of a BCVR, wherein the ACVR and BCVR are matched.
102. The method of paragraph 101, wherein the AC element comprises, or consists of, an
ACVRS, or a functional fragment thereof (e.g., an antigen binding fragment thereof).
103. The method of paragraph 101 or 102, wherein the BC element comprises, or consists of,
a BCVRS, or a functional fragment thereof (e.g., an antigen binding fragment thereof).
104. The method of any of paragraphs 101-103, wherein the nucleic acid sequence is
red such that, when expressed, the ACVRS and the BCVRS form a functional antigen binding
molecule, e. g., a single chain TCR molecule.
105. The method of paragraph 104, wherein the antigen binding molecule is functional in
vitro, ex vivo, or in viva, e.g., as determined by a method or assay described herein.
106. The method of any of paragraphs 101-105, wherein acquiring an ed production
reaction site, e.g., a production micro-chamber, comprises:
a) acquiring a capture ate bound to:
(i) a first double-stranded cDNA (ds cDNA) comprising a strand that is
complementary to a first mRNA that encodes an ACVR from a cell; and
(ii) a second ds cDNA comprising a strand complementary to a second mRNA
encoding a BCVR from the cell (the cDNA loaded capture substrate), and
b) ining the isolated tion reaction site, e.g., the production micro-chamber,
under conditions that allow cation of the first and second ds cDNAs, to produce:
a plurality of AC ds cDNAs comprising a segment that encodes an AC element of the ACVR
from the cell, e.g., an ACVRS; and
a plurality of BC ds cDNAs comprising a segment that encodes a BC element of the BCVR
from the cell, e.g., a BCVRS.
107. The method of paragraph 106, wherein the AC ds cDNA is identical, or substantially
identical, to the first ds cDNA.
108. The method of paragraph 106 or 107, n the BC ds cDNA is identical, or
substantially identical, to the second ds cDNA.
109. The method of any of paragraphs 106-108, wherein the e substrate comprises a
bead, e.g., a magnetic bead.
110. The method of any of paragraphs 106-109, wherein the capture substrate comprises a
moiety (e.g., an ucleotide) which binds to cDNA, e.g., (i) a moiety which binds to the AC
strand; (ii) a moiety which binds to the BC strand; or (iii) both (i) and (ii).
111. The method of any of paragraphs 106-110, wherein the first mRNA and the second
mRNA are disposed on an mRNA loaded capture ate.
112. The method of any of paragraphs 106-111, wherein the isolated production reaction site,
e.g., the production micro-chamber, comprises:
a reagent mixture suitable for producing, from the first and second mRNAs (e.g., after the
first and second mRNAs are released from the mRNA loaded capture substrate into a solution), a first
ds cDNA comprising a segment that encodes an AC element of the ACVR of the cell, e. g., an
ACVRS, and a second ds cDNA comprising a segment that encodes a BC element of the BCVR of the
cell, e.g., a BCVRS.
113. The method of any of aphs 106-112, wherein the isolated tion reaction site,
e.g., production micro-chamber, comprises primers that mediate the production of the first ds cDNA.
114. The method of any of paragraphs 106-113, wherein the isolated production reaction site,
e.g., production micro-chamber, comprises primers that mediate the production of the second ds
cDNA.
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115. The method of any of paragraphs 106-114, wherein a cDNA strand that is
complementary to a first mRNA that encodes an ACVR from a cell is made by reverse transcription
of the first mRNA.
116. The method of any of paragraphs 106-115, n a cDNA strand that is
complementary to a second mRNA that encodes a BCVR from a cell is made by reverse transcription
of the second mRNA.
117. The method of paragraph 115 or 116, wherein the reverse transcription takes place in
the isolated production reaction site, e.g., a production-micro chamber.
118. The method of paragraph 115 or 116, wherein the reverse transcription takes place in an
isolated cell reaction site, e.g., a cell isolation micro-chamber.
119. The method of paragraph 115 or 116, wherein the reverse transcription takes place
outside the isolated production reaction site, e.g., a production micro-chamber, or outside an isolated
cell reaction site, e.g., a cell isolation micro-chamber.
120. The method of paragraph 115 or 116, wherein the e transcription takes place
outside the isolated production on site, e.g., a production-micro chamber, and outside an isolated
cell reaction site, e.g., a cell ion micro-chamber.
121. The method of paragraph 115 or 116, wherein the reverse ription takes place
outside an ed reaction site, e.g., e a micro-chamber.
122. The method of any of paragraphs 106-121, n the amplification comprises 20 or
fewer cycles, e.g., 15 or fewer, 14 or fewer, 13 or fewer, 12 or fewer, 11 or fewer, 10 or fewer, 9 or
fewer, 8 or fewer, 7 or fewer, 6 or fewer, or 5 or fewer cycles.
123. The method of any of paragraphs 106-122, wherein the reverse transcription and/or
amplification uses one or more primers, e.g., comprising a sequence specific for an ACVRS and/or a
BCVRS.
124. The method of any of paragraphs 106-123, n the amplification comprises using
two or more primers that mediate the production of the AC ds cDNA, wherein at least one primer
comprises a nucleotide modification, and wherein at least one primer does not comprise a nucleotide
modification.
125. The method of any of paragraphs 106-124, wherein the amplification comprises using
two or more primers that mediate the production of the BC ds cDNA, wherein at least one primer
comprises a nucleotide modification, and n at least one primer does not comprise a nucleotide
modification.
126. The method of aph 125, wherein at least one primer comprises a nucleotide
modification, e.g., which reduces, e.g., inhibits, DNA synthesis, e.g., by a DNA polymerase.
127. The method of paragraph 125 or 126, wherein at least one primer does not comprise a
nucleotide modification, e.g., which reduces, e.g., inhibits, DNA synthesis, e.g., by a DNA
polymerase.
128. The method of paragraph 126 or 127, wherein the nucleotide cation inhibits a
DNA polymerase from extending the DNA.
129. The method of any of paragraphs 126-128, wherein the nucleotide modification is an
insertion of a spacer to the primer, e.g., between two adjacent nucleotides in the primer.
130. The method of paragraph 129, wherein the spacer is a flexible spacer, a carbon spacer
(e.g., -(CH2)n-, wherein n=3, 4, 5, or more), two or more (e.g., three, four, five, or more) abasic
nucleotides or a PEG .
131. The method of any of paragraphs 8, wherein the nucleotide modification is 2’-O-
methyl, 2’-OH, 2’-NH2, or uracil, e.g., to a ribose.
132. The method of any of aphs 126-131, wherein the nucleotide ation is
located internally or at the 3’ end of the primer.
133. The method of any of paragraphs 123-132, wherein at least one primer comprises (i) a
first member; (ii) a second member; and optionally (iii) a nucleotide modification described herein,
e. g., located between (i) and (ii).
134. The method of paragraph 133, wherein the first member is capable of ing with the
second member in the same primer or a different primer, e.g., forming a n structure (via
intramolecular hybridization) or a double-stranded structure (via intermolecular hybridization)
comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more
basepairs.
135. The method of paragraph 133 or 134, wherein the first member comprises a sequence
that is complementary to the sequence of an oligonucleotide attached to the capture substrate.
136. The method of any of paragraphs 133-135, wherein the second member ses (e.g.,
from 5’ to 3’) one, two, or all of: (i) a sequence that is complementary to at least a n of the first
member; (ii) a universal priming sequence (e.g., for PCR amplification or next-generation
cing); and (iii) a sequence complementary to a target sequence, e. g., an ACVRS and/or a
BCVRS, optionally, wherein the second member comprises a sequence for homologous
recombination (e.g., in a yeast or mammalian cell).
137. The method of any of paragraphs 123-136, wherein at least one primer comprises a
sequence encoding at least a portion of a linker sequence, or a mentary ce thereof.
138. The method of paragraph 137, wherein the primer that comprises a sequence encoding
at least a n of a linker sequence, or a complementary sequence thereof, is phosphorylated, e.g.,
’ phosphorylated.
139. The method of paragraph 137 or 138, wherein the linker sequence ses, or consists
of, (Gly-Gly-Gly-Gly-Ser)n, where n=1, 2, 3, 4, 5, or more.
140. The method of any of paragraphs 101-139, wherein the AC ds cDNA comprises a 5’
overhang, e.g., a 5’ ng that is capable of hybridizing to an oligonucleotide attached to a capture
substrate.
141. The method of any of paragraphs 101-140, wherein the AC ds cDNA comprises a blunt
end, e.g., a blunt end comprising a 5’ phosphate.
142. The method of any of paragraphs 101-141, wherein the BC ds cDNA comprises a 5’
overhang, e.g., a 5’ overhang that is capable of hybridizing to an oligonucleotide attached to a capture
substrate.
143. The method of any of paragraphs 2, wherein the BC ds cDNA comprises a blunt
end, e.g., a blunt end comprising a 5’ phosphate.
144. The method of any of paragraphs 101-143, wherein the AC ds cDNA and the BC ds
cDNA comprise sticky ends, e.g., both have 5’ overhangs.
145. The method of any of paragraphs 101-144, wherein the AC strand and the BC strand are
covalently linked, e.g., ligated, to produce a single stranded nucleic acid sequence, wherein the AC
and BC strands are both sense strands or both antisense strands.
146. The method of any of aphs 101-144, wherein a denatured AC strand of the AC ds
cDNA to a denatured BC strand of the BC ds cDNA are ntly linked, e.g., ligated, wherein the
AC and BC strands are both sense strands or both antisense strands.
147. The method of any of aphs 101-144, wherein the AC strand is present in the AC
ds cDNA and the BC strand is present in the BC ds cDNA, and wherein the AC ds cDNA and the BC
ds cDNA are covalently linked, e.g., ligated, e. g., to produce a double stranded nucleic acid sequence.
148. The method of any of paragraphs 101-147, wherein the covalent linking, e.g., ligation,
occurs in the isolated production reaction site.
149. The method of paragraph 148, wherein the isolated production on site, e.g., a
production micro-chamber, ses a reagent that is capable of covalently linking, e. g., ligating, the
AC and BC s or the AC and BC ds cDNAs.
150. The method of paragraph 148 or 149, wherein the isolated production reaction site, e.g.,
a production chamber comprises an enzyme that covalently couples the AC and BC strands or
the AC and BC ds cDNAs.
151. The method of any of paragraphs 101-147, wherein the covalent linking, e. g., ligation,
occurs in a site different from the isolated production reaction site, e.g., occurs in an isolated e
reaction site, e.g., a linkage chamber.
152. The method of paragraph 151, wherein the AC strand and the BC strand are transferred
from the isolated production site to the isolated linkage reaction site, e.g., a e micro-chamber,
and the covalent linking occurs in the isolated linkage reaction site, e.g., a linkage micro-chamber.
153. The method of paragraph 151 or 152, wherein the isolated linkage reaction site, e.g., a
linkage chamber, ses a reagent that is capable of covalently linking, e.g., ligating, the
AC and BC strands or the AC and BC ds cDNAs.
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154. The method of any of paragraphs 151-153, wherein the ed linkage reaction site,
e.g., a linkage micro-chamber, comprises an enzyme that covalently couples the AC and BC strands
or the AC and BC ds cDNAs.
155. The method of paragraph 150 or 154, wherein the enzyme is a ligase, e.g., a l
stable ligase.
156. The method of any of aphs 151-155, wherein the covalent linking, e.g., ligation,
comprises:
(a) heating the isolated linkage reaction site, e.g., the e micro-chamber, under
conditions (e.g., at 95°C) that allow denaturation of the AC strand and the BC strand;
(b) cooling the isolated linkage reaction site, e.g., the linkage micro-chamber, under
conditions (e.g., at C) that allow ization of the splint oligonucleotide to the AC strand
and the BC strand;
(c) maintaining the isolated linkage reaction site, e.g., the linkage chamber, under
conditions (e.g., at 45-65°C) that allow ligation of the AC strand and the BC strand (e.g., formation of
phosphodiester bond n the AC strand and the BC strand); and
(d) repeating steps (a), (b), and (c) sequentially for 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, or
more cycles.
157. The method of any of paragraphs 101-156, wherein the AC strand and the BC strand are
covalently linked, e.g., ligated, in the presence of a splint oligonucleotide.
158. The method of paragraph 157, wherein the splint oligonucleotide is hybridized to a
sequence comprising the junction of the AC strand and the BC strand, or a sequence complementary
thereof, and forms a duplexed region at the site of ligation.
159. The method of paragraph 157 or 158, wherein the splint oligonucleotide comprises a
modification (e.g., an NH2 group) that inhibits DNA synthesis, e.g., by a DNA polymerase.
160. The method of paragraph 159, wherein the modification is at the 3’ end of the splint
oligonucleotide.
161. The method of any of paragraphs 101-160, wherein a strand complimentary to the
covalently linked, e.g., ligated, AC and BC s is produced by amplification.
162. The method of any of paragraphs 101-161, further comprising, prior to ing the
isolated production reaction site, e.g., a production micro-chamber, acquiring an mRNA loaded
capture substrate.
163. The method of paragraph 162, wherein ing the mRNA loaded capture substrate
comprising:
a) acquiring an isolated cell reaction site, e.g., a cell isolation micro-chamber, sing:
i) a cell; and
ii) a capture substrate capable of binding a first mRNA encoding an ACVR from the
cell and a second mRNA encoding a BCVR from the cell; and
b) maintaining the isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a
nucleic acid encoding an ACVR or a BCVR from a cell other than the cell.
164. The method of paragraph 163, wherein the isolated cell reaction site, e.g., cell isolation
micro-chamber, comprises a lysing reagent, e.g., a detergent.
165. The method of paragraph 163 or 164, wherein the cell is lysed by heat or an enzyme.
166. The method of any of paragraphs 163-165, wherein the capture ate comprises a
moiety (e.g., an oligonucleotide) which binds mRNA, e.g., an oligo(dT).
167. The method of any of paragraphs 162-166, r comprising releasing the mRNA
loaded e substrate from the isolated cell reaction site, e.g., the cell isolation micro-chamber.
168. The method of paragraph 167, wherein the releasing step is performed in the presence of
a poly(dA) or poly(dT) oligonucleotide, e.g., to reduce cross-binding of non-captured mRNA.
169. The method of aph 162-168, wherein the mRNA loaded capture substrate is
transferred from the isolated cell reaction site, e.g., the cell isolation micro-chamber, to the isolated
production on site, e.g., the production micro-chamber.
170. The method of any of paragraphs 101-169, comprising releasing the nucleic acid
sequence from the production chamber.
171. The method of any of paragraphs 101-170, further comprising ying the nucleic
acid sequence.
172. The method of any of paragraphs 101-171, comprising sequencing all or a n of the
c acid sequence.
173. The method of any of paragraphs , comprising inserting all or a portion of
nucleic acid sequence into a .
174. The method of paragraph 173, wherein the vector supplies an onal AC element or
BC element not included in the nucleic acid sequence.
175. The method of paragraph 173 or 174, wherein the vector supplies an AC CDRl, an AC
CDR2, or both.
176. The method of any of paragraphs 173-175, comprising expressing the vector.
177. The method of any of aphs 101-176, comprising sing the nucleic acid
sequence to produce a polypeptide comprising a segment that encodes an AC element of the ACVR,
e. g., an ACVRS, and a segment that encodes a BC element of the BCVR, e. g., a BCVRS.
178. The method of paragraph 177, wherein the BC element is N—terminal to the AC element
in the polypeptide.
179. The method of paragraph 177, wherein the AC element is C-terminal to the BC element
in the polypeptide.
180. The method of any of paragraphs 177-179, further comprising contacting the
polypeptide with an antigen.
181. The method of any of paragraphs 177-180, further comprising ining if the
polypeptide binds the antigen.
182. The method of any of paragraphs 101-181, wherein the cell is an immune cell, e.g., a B
cell or T cell, e.g., a human B cell or T cell.
183. The method of any of paragraphs 101-182, wherein the cell is a ian cell or an
avian cell.
184. A method of making a nucleic acid sequence comprising a sequence that encodes an 0:
chain element (AC element) of a TCR 0t chain variable region (ACVR) and a [3 chain element (BC
element) of a TCR [3 chain le region (BCVR), and wherein the ACVR and BCVR are matched,
comprising:
a) acquiring an isolated cell reaction site, e.g., a cell isolation micro-chamber, comprising:
i) a cell; and
ii) a capture substrate capable of g a first mRNA encoding an ACVR from the
cell and a second mRNA encoding a BCVR from the cell;
b) maintaining isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e. g., cell isolation micro-chamber, does not include a
nucleic acid encoding an ACVR or a BCVR from a cell other than the cell;
c) contacting the mRNA loaded capture ate with a reaction mixture, e.g., a reaction
mixture comprising reverse transcriptase, that uses the loaded mRNA as a template to make cDNA
(this can occur, e.g., in the ed cell reaction site, in the ed production reaction site, or in
neither, e.g., not in an isolated reaction site);
d) acquiring an isolated production reaction site, e.g., a production chamber,
sing:
i) an on chain (AC) strand from step b), wherein the AC strand is a strand of an 0t chain
double-stranded cDNA (AC ds cDNA) sing a segment that encodes an AC element of
the ACVR from the cell, e.g., a 0L chain variable region sequence (ACVRS); and
ii) a [3 chain (BC) strand from step b), wherein the BC strand is a strand of a [3 chain
double-stranded cDNA (BC ds cDNA) comprising a segment that encodes a BC element of
the BCVR from the cell, e.g., a B chain variable region sequence (BCVRS); and
e) covalent linking, e.g., ligation, of an AC strand to a BC ,
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
e a nucleic acid encoding a BCVR or an ACVR from a cell other than the cell.
185. A method of making a nucleic acid sequence comprising a sequence that s a or
chain element (AC element) of an TCR or chain le region (ACVR) and a [3 chain element (BC
element) of an TCR B chain variable region (BCVR), and wherein the ACVR and BCVR are d,
comprising:
a) acquiring an isolated cell reaction site, e.g., a cell isolation chamber, comprising:
i) a cell; and
ii) a capture substrate capable of binding a first mRNA encoding an ACVR from the
cell and a second mRNA encoding a BCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell ion micro-chamber, under
conditions that allow lysis of the cell and binding of the capture ate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell on site, e.g., cell isolation micro-chamber, does not include a
nucleic acid encoding an ACVR or a BCVR from a cell other than the cell;
c) acquiring an isolated production reaction site, e. g., a production micro-chamber, comprises:
ting the mRNA loaded capture substrate with a reaction mixture, e.g., a reaction mixture
comprising e transcriptase, that uses the loaded mRNA as a template, to produce:
a first double-stranded cDNA (ds cDNA) comprising a strand that is complementary to a first
mRNA that encodes an ACVR from a cell; and
a second ds cDNA comprising a strand complementary to a second mRNA encoding a BCVR
from the cell (the cDNA loaded capture substrate);
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding a BCVR or an ACVR from a cell other than the cell.
d) maintaining the isolated production reaction site, e.g., the production micro-chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce:
a plurality of AC ds cDNAs comprising a segment that s an AC t of the ACVR
from the cell, e.g., an ACVRS; and
a plurality of BC ds cDNAs comprising a segment that encodes a BC element of the BCVR
from the cell, e.g., a BCVRS;
e) acquiring an isolated linkage reaction site, e.g., a linkage micro-chamber, comprising:
covalent linking, e.g., ligation, of a strand of the AC ds cDNA (AC strand) to a strand of the BC ds
cDNA (BC strand), wherein the AC and BC strands are both sense strands or antisense strands; and
f) amplifying the covalently linked, e.g., d, AC and BC strands.
186. A method of making a y comprising a plurality of unique members, the method
comprising:
making the plurality of members, n each of the members comprises a sequence that
encodes a 0t chain element (AC element) of a 0t chain variable region (ACVR) and a [3 chain element
(BC element) of a [3 chain variable region (BCVR), and wherein the ACVR and BCVR are matched,
made by a method of any of paragraphs 101-185,
wherein each unique nucleic acid sequence of the plurality comprises an AC element and a
BC element from a different unique cell,
thereby making a library sing a plurality of unique members.
187. The method of paragraph 186, wherein the plurality of unique members ses at
least 104, 105, 106, 107, 108, or 109 unique members.
188. The method of paragraph 186 or 187, wherein the plurality of unique members
comprises 104 to 109, 104 to 108, 104 to 107, 104 to 106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105
to 109, 105 to 108, 106 to 107, 104 to 105, 105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique
members.
189. The method of any of paragraphs 186-188, wherein at least 80%, 85%, 90%, 95%, 96%,
97%, 98%, 99%, or 100%, of the members in the library are unique members (which encode d
AC element and BC elements sequences).
190. The method of any of paragraphs 186-189, wherein less than 20%, 15%, 10%, 5%, 4%,
3%, 2%, or 1%, of the s in the library are unique members (which encode matched AC
element and BC elements sequences).
191. A library comprising:
a plurality of unique members,
wherein,
i) each unique member of the plurality comprises a segment that encodes an AC element, e.g.,
an ACVRS, and a segment that encodes a BC element, e.g., a BCVRS, wherein the AC element and
the BC t in each unique member is d;
ii) each unique member of the plurality comprises a t that encodes an AC element,
e.g., an ACVRS, and a segment that s a BC t, e.g., a BCVRS, from a different unique
cell; and
iii) the library comprises one or more (e.g., two, three, four, or all) of the following
properties:
a) the library is made by a method of any of paragraphs 101-185;
b) the plurality of unique members comprises at least 104, 105, 106, 107, 108, or 109
unique nucleic acid sequences;
c) the plurality of unique members comprises 104 to 109, 104 to 108, 104 to 107, 104 to
106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 108, 106 to 107, 104 to 105,
105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique members;
d) at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the members in
the library are unique members (which encode matched AC element and BC elements
sequences); or
e) less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the s in the library
are unique members (which encode matched AC element and BC elements sequences).
192. The library of paragraph 191, wherein each unique member of the plurality is
configured such that, when expressed, the AC element, e.g., the ACVRS, and the BC element, e.g.,
the BCVRS, form a functional antigen binding molecule, e.g., a single chain TCR.
193. The library of any of paragraphs 191-192, wherein the y is a y library.
194. The library of any of paragraphs 3, wherein each of the members of the plurality
further encodes a polypeptide that results in display of the member on the surface of a display entity.
195. The library of any of paragraphs 191-194, wherein the library is a phage display library.
196. The y of any of aphs 191-194, wherein the library is a yeast display library.
197. The library of any of paragraphs 191-194, wherein the library is a mammalian display
library.
198. A method of making a binding polypeptide, the method comprising:
a) ing a library of any of paragraphs 191-197; and
b) expressing a polypeptide encoded by a unique nucleic acid of the y.
199. The method of paragraph 198, further sing contacting the polypeptide with an
antigen.
200. The method of paragraph 198 or 199, further sing retrieving the nucleic acid that
s a polypeptide that binds the antigen.
201. A method of making a nucleic acid sequence sing a sequence that encodes an 7
chain element (GC element) of a TCR 7 chain variable region (GCVR) and a 5 chain element (DC
element) of a TCR 5 chain variable region (DCVR), and wherein the GCVR and DCVR are matched,
the method comprising:
a) acquiring an isolated production reaction site, e.g., a production micro-chamber,
comprising:
i) a 7 chain (GC) strand, wherein the GC strand is a strand of a 7 chain double-
stranded cDNA (GC ds cDNA) comprising a t that encodes a GC element of the
GCVR from a cell, e.g., a 7 chain variable region ce (GCVRS); and
ii) a 5 chain (DC) strand, wherein the DC strand is a strand of a 5 chain double-
stranded cDNA (DC ds cDNA) comprising a segment that encodes a DC element of the
DCVR from the cell, e.g., a 5 chain variable region sequence (DCVRS), and
b) covalent linking, e.g., ligation, of a GC strand to a DC strand,
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding a DCVR or a GCVR from a cell other than the cell,
thereby making a nucleic acid ce comprising a sequence that encodes a GC element of
a GCVR and a DC element of a DCVR, wherein the GCVR and DCVR are matched.
202. The method of paragraph 201, wherein the GC element comprises, or ts of, a
GCVRS, or a functional fragment f (e. g., an antigen binding fragment thereof).
203. The method of paragraph 201 or 202, wherein the DC element ses, or consists of,
a DCVRS, or a functional fragment thereof (e.g., an antigen g nt thereof).
204. The method of any of aphs 201-203, wherein the nucleic acid sequence is
configured such that, when sed, the GCVRS and the DCVRS form a functional antigen binding
molecule, e. 3., a single chain TCR molecule.
205. The method of paragraph 204, wherein the antigen binding molecule is functional in
vitro, ex vivo, or in viva, e.g., as determined by a method or assay described herein.
206. The method of any of paragraphs 201-205, wherein acquiring an isolated production
reaction site, e.g., a production micro-chamber, comprises:
a) acquiring a capture substrate bound to:
(i) a first double-stranded cDNA (ds cDNA) comprising a strand that is
complementary to a first mRNA that encodes a GCVR from a cell; and
(ii) a second ds cDNA sing a strand complementary to a second mRNA
encoding a DCVR from the cell (the cDNA loaded capture substrate), and
b) maintaining the ed production reaction site, e.g., the production micro-chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce:
a plurality of GC ds cDNAs comprising a segment that encodes a GC element of the GCVR
from the cell, e. g., a GCVRS; and
a plurality of DC ds cDNAs comprising a segment that encodes a DC element of the DCVR
from the cell, e.g., a DCVRS.
207. The method of paragraph 206, wherein the GC ds cDNA is identical, or substantially
identical, to the first ds cDNA.
208. The method of paragraph 206 or 207, wherein the DC ds cDNA is identical, or
substantially identical, to the second ds cDNA.
209. The method of any of paragraphs 206-208, n the capture substrate comprises a
bead, e.g., a magnetic bead.
210. The method of any of paragraphs 206-209, wherein the capture substrate comprises a
moiety (e.g., an oligonucleotide) which binds to cDNA, e.g., (i) a moiety which binds to the GC
; (ii) a moiety which binds to the DC strand; or (iii) both (i) and (ii).
211. The method of any of paragraphs 0, wherein the first mRNA and the second
mRNA are disposed on an mRNA loaded capture substrate.
212. The method of any of paragraphs 206-211, wherein the isolated production reaction site,
e.g., the production micro-chamber, comprises:
a reagent mixture suitable for producing, from the first and second mRNAs (e.g., after the
first and second mRNAs are released from the mRNA loaded capture substrate into a solution), a first
ds cDNA sing a segment that encodes a GC element of the GCVR of the cell, e.g., a GCVRS,
and a second ds cDNA comprising a segment that encodes a DC element of the DCVR of the cell,
e.g., a DCVRS.
213. The method of any of paragraphs 206-212, n the isolated production reaction site,
e.g., production micro-chamber, ses primers that mediate the production of the first ds cDNA.
214. The method of any of paragraphs 206-213, n the isolated production reaction site,
e.g., tion micro-chamber, comprises primers that mediate the production of the second ds
cDNA.
215. The method of any of paragraphs 206-214, wherein a cDNA strand that is
complementary to a first mRNA that encodes a GCVR from a cell is made by reverse transcription of
the first mRNA.
216. The method of any of paragraphs 206-215, wherein a cDNA strand that is
complementary to a second mRNA that encodes a DCVR from a cell is made by reverse transcription
of the second mRNA.
217. The method of paragraph 215 or 216, wherein the reverse transcription takes place in
the isolated production reaction site, e.g., a production-micro r.
218. The method of paragraph 215 or 216, wherein the reverse transcription takes place in an
isolated cell reaction site, e. g., a cell isolation micro-chamber.
219. The method of paragraph 215 or 216, wherein the reverse transcription takes place
outside the isolated production reaction site, e.g., a production micro-chamber, or outside an isolated
cell reaction site, e.g., a cell ion micro-chamber.
220. The method of paragraph 215 or 216, wherein the reverse transcription takes place
outside the isolated production reaction site, e.g., a production-micro chamber, and outside an isolated
cell reaction site, e.g., a cell isolation micro-chamber.
221. The method of paragraph 215 or 216, wherein the reverse transcription takes place
outside an isolated reaction site, e.g., e a micro-chamber.
222. The method of any of paragraphs 206-221, wherein the amplification comprises 20 or
fewer cycles, e.g., 25 or fewer, 24 or fewer, 23 or fewer, 22 or fewer, 21 or fewer, 20 or fewer, 9 or
fewer, 8 or fewer, 7 or fewer, 6 or fewer, or 5 or fewer cycles.
223. The method of any of paragraphs 206-222, n the reverse transcription and/or
amplification uses one or more primers, e.g., sing a sequence c for a GCVRS and/or a
DCVRS.
224. The method of any of paragraphs 206-223, wherein the amplification ses using
two or more primers that mediate the production of the GC ds cDNA, wherein at least one primer
comprises a nucleotide modification, and wherein at least one primer does not comprise a tide
modification.
225 . The method of any of paragraphs 206-224, wherein the amplification comprises using
two or more primers that mediate the production of the DC ds cDNA, wherein at least one primer
comprises a nucleotide modification, and wherein at least one primer does not comprise a nucleotide
modification.
226. The method of paragraph 225, wherein at least one primer comprises a nucleotide
modification, e.g., which reduces, e.g., inhibits, DNA synthesis, e.g., by a DNA polymerase.
227. The method of paragraph 225 or 226, wherein at least one primer does not comprise a
nucleotide modification, e.g., which reduces, e.g., inhibits, DNA synthesis, e.g., by a DNA
polymerase.
228. The method of paragraph 226 or 227, wherein the nucleotide modification inhibits a
DNA rase from extending the DNA.
229. The method of any of paragraphs 226-228, wherein the nucleotide modification is an
insertion of a spacer to the primer, e.g., between two adjacent nucleotides in the primer.
230. The method of paragraph 229, wherein the spacer is a flexible spacer, a carbon spacer
(e.g., n-, wherein n=3, 4, 5, or more), two or more (6.3., three, four, five, or more) abasic
tides or a PEG spacer.
231. The method of any of paragraphs 226-228, wherein the nucleotide modification is 2’-O-
methyl, 2’-OH, 2’-NH2, or uracil, e.g., to a ribose.
232. The method of any of paragraphs 226-231, wherein the nucleotide modification is
d ally or at the 3’ end of the primer.
233. The method of any of paragraphs 223-232, wherein at least one primer ses (i) a
first member; (ii) a second ; and optionally (iii) a nucleotide modification described herein,
e.g., located between (i) and (ii).
234. The method of paragraph 233, wherein the first member is capable of annealing with the
second member in the same primer or a different primer, e.g., forming a hairpin ure (Via
intramolecular hybridization) or a double-stranded ure (Via intermolecular hybridization)
comprising a duplex region of 4, 5, 6, 7, 8, 9, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 20, more
basepairs.
235 . The method of paragraph 233 or 234, n the first member comprises a sequence
that is mentary to the sequence of an oligonucleotide attached to the capture substrate.
236. The method of any of paragraphs 233-235, wherein the second member comprises (6g,
from 5 ’ to 3’) one, two, or all of: (i) a ce that is complementary to at least a portion of the first
member; (ii) a universal priming sequence (e.g., for PCR ication or next-generation
sequencing); and (iii) a sequence complementary to a target sequence, e.g., a GCVRS and/or a
DCVRS, optionally, wherein the second member comprises a sequence for homologous
recombination (e.g., in a yeast or mammalian cell).
237. The method of any of paragraphs 223-236, wherein at least one primer comprises a
sequence encoding at least a n of a linker sequence, or a complementary sequence thereof.
238. The method of paragraph 237, n the primer that comprises a sequence encoding
at least a portion of a linker sequence, or a complementary sequence thereof, is phosphorylated, e.g.,
’ phosphorylated,
239. The method of paragraph 237 or 238, wherein the linker sequence comprises, or consists
of, (Gly-Gly-Gly-Gly-Ser)n, where n=1, 2, 3, 4, 5, or more.
240. The method of any of paragraphs 201-239, n the GC ds cDNA ses a 5’
overhang, e.g., a 5’ overhang that is capable of izing to an oligonucleotide attached to a capture
substrate.
241. The method of any of paragraphs 201-240, wherein the GC ds cDNA comprises a blunt
end, e.g., a blunt end comprising a 5’ phosphate.
242. The method of any of paragraphs 201-241, wherein the DC ds cDNA comprises a 5’
overhang, e.g., a 5’ overhang that is capable of hybridizing to an oligonucleotide attached to a capture
substrate.
243. The method of any of paragraphs 2, wherein the DC ds cDNA comprises a blunt
end, e.g., a blunt end comprising a 5’ phosphate.
244. The method of any of paragraphs 201-243, wherein the GC ds cDNA and the DC ds
cDNA comprise sticky ends, e.g., both have 5’ overhangs.
245. The method of any of paragraphs 201-244, wherein the GC strand and the DC strand are
covalently linked, e.g., ligated, to produce a single stranded nucleic acid ce, wherein the GC
and DC strands are both sense strands or both antisense strands.
246. The method of any of paragraphs 201-245, wherein a denatured GC strand of the GC ds
cDNA to a denatured DC strand of the DC ds cDNA are covalently linked, e.g., ligated, n the
GC and DC strands are both sense s or both antisense strands.
247. The method of any of paragraphs 201-245, wherein the GC strand is present in the GC
ds cDNA and the DC strand is present in the DC ds cDNA, and wherein the GC ds cDNA and the DC
ds cDNA are covalently linked, e.g., ligated, e.g., to produce a double stranded nucleic acid sequence.
248. The method of any of paragraphs 7, wherein the covalent linking, e. g., ligation,
occurs in the isolated production reaction site.
249. The method of paragraph 248, wherein the ed production reaction site, e.g., a
production micro-chamber, comprises a reagent that is capable of covalently linking, e.g., ligating, the
GC and DC strands or the GC and DC ds cDNAs.
250. The method of paragraph 248 or 249, wherein the isolated production reaction site, e.g.,
a production micro-chamber comprises an enzyme that ntly couples the GC and DC strands or
the GC and DC ds cDNAs.
251. The method of any of paragraphs 201-247, wherein the covalent g, e.g., ligation,
occurs in a site different from the isolated production reaction site, e.g., occurs in an ed linkage
reaction site, e.g., a linkage micro-chamber.
252. The method of paragraph 251, wherein the GC strand and the DC strand are transferred
from the isolated production site to the ed linkage reaction site, e. g., a linkage micro-chamber,
and the covalent linking occurs in the isolated linkage reaction site, e.g., a linkage micro-chamber.
253. The method of paragraph 251 or 252, wherein the isolated linkage reaction site, e.g., a
linkage micro-chamber, comprises a reagent that is capable of covalently linking, e.g., ligating, the
GC and DC strands or the GC and DC ds cDNAs.
254. The method of any of paragraphs 25 1-25 3, wherein the isolated linkage reaction site,
e.g., a e micro-chamber, comprises an enzyme that covalently s the GC and DC strands
or the GC and DC ds cDNAs.
255. The method of paragraph 250 or 254, wherein the enzyme is a ligase, e.g., a thermal
stable ligase.
256. The method of any of paragraphs 251-255, wherein the covalent linking, e.g., ligation,
comprises:
(a) heating the isolated linkage reaction site, e.g., the linkage micro-chamber, under
conditions (e.g., at 96°C) that allow ration of the GC strand and the DC strand;
(b) cooling the isolated linkage reaction site, e.g., the linkage micro-chamber, under
conditions (e.g., at 60-66°C) that allow hybridization of the splint oligonucleotide to the GC strand
and the DC ;
(c) maintaining the isolated linkage reaction site, e.g., the linkage chamber, under
conditions (e.g., at 46-66°C) that allow ligation of the GC strand and the DC strand (e.g., formation of
phosphodiester bond between the GC strand and the DC strand); and
(d) repeating steps (a), (b), and (c) sequentially for 2, 6, 20, 26, 20, 26, 30, 36, 40, 46, 60, or
more cycles.
257. The method of any of aphs 201-256, wherein the GC strand and the DC strand are
covalently linked, e.g., ligated, in the presence of a splint oligonucleotide.
258. The method of paragraph 257, wherein the splint oligonucleotide is ized to a
sequence comprising the on of the GC strand and the DC strand, or a ce complementary
thereof, and forms a duplexed region at the site of on.
259. The method of aph 257 or 258, wherein the splint oligonucleotide comprises a
modification (e.g., an NHZ group) that inhibits DNA synthesis, e.g., by a DNA polymerase.
260. The method of paragraph 259, wherein the modification is at the 3’ end of the splint
oligonucleotide.
261. The method of any of paragraphs 201-260, wherein a strand complimentary to the
covalently linked, e. g., ligated, GC and DC s is produced by amplification.
262. The method of any of paragraphs 201-261, further comprising, prior to acquiring the
isolated production reaction site, e.g., a production micro-chamber, acquiring an mRNA loaded
capture substrate.
263. The method of paragraph 262, wherein acquiring the mRNA loaded capture substrate
comprising:
a) acquiring an ed cell reaction site, e.g., a cell ion micro-chamber, comprising:
i) a cell; and
ii) a capture substrate capable of binding a first mRNA encoding a GCVR from the
cell and a second mRNA encoding a DCVR from the cell; and
b) maintaining the ed cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell on site, e.g., cell isolation micro-chamber, does not e a
nucleic acid encoding a GCVR or a DCVR from a cell other than the cell.
264. The method of paragraph 263, wherein the isolated cell on site, e.g., cell isolation
micro-chamber, comprises a lysing reagent, e.g., a detergent.
265. The method of paragraph 263 or 264, n the cell is lysed by heat or an enzyme.
266. The method of any of paragraphs 263-265, wherein the capture substrate ses a
moiety (e.g., an oligonucleotide) which binds mRNA, e.g., an oligo(dT).
267. The method of any of paragraphs 262-266, further comprising releasing the mRNA
loaded capture substrate from the isolated cell reaction site, e.g., the cell isolation micro-chamber.
268. The method of aph 267, wherein the releasing step is performed in the presence of
a poly(dA) or poly(dT) ucleotide, e.g., to reduce cross-binding of ptured mRNA.
269. The method of paragraph 262-268, wherein the mRNA loaded capture substrate is
transferred from the isolated cell reaction site, e.g., the cell isolation micro-chamber, to the isolated
production reaction site, e.g., the production micro-chamber.
270. The method of any of paragraphs 201-269, comprising releasing the nucleic acid
sequence from the production micro-chamber.
271. The method of any of paragraphs 201-270, further comprising amplifying the nucleic
acid sequence.
272. The method of any of paragraphs 201-271, comprising sequencing all or a portion of the
nucleic acid sequence.
273. The method of any of aphs 201-272, comprising inserting all or a portion of
nucleic acid sequence into a vector.
274. The method of paragraph 273, wherein the vector supplies an onal GC element or
DC element not included in the nucleic acid sequence.
275. The method of paragraph 273 or 274, n the vector supplies a GC CDRl, a GC
CDR2, or both.
276. The method of any of aphs 273-275, comprising expressing the vector.
277. The method of any of paragraphs 201-276, comprising expressing the nucleic acid
sequence to produce a ptide comprising a segment that encodes a GC element of the GCVR,
e.g., a GCVRS, and a segment that encodes a DC element of the DCVR, e.g., a DCVRS.
278. The method of paragraph 277, wherein the DC element is N—terminal to the GC element
in the ptide.
279. The method of paragraph 277, wherein the GC element is C-terminal to the DC element
in the polypeptide.
280. The method of any of paragraphs 277-279, r comprising contacting the
polypeptide with an antigen.
281. The method of any of paragraphs 0, r comprising determining if the
polypeptide binds the antigen.
282. The method of any of paragraphs 201-281, wherein the cell is an immune cell, e.g., a B
cell or T cell, e.g., a human B cell or T cell.
283. The method of any of paragraphs 201-282, wherein the cell is a mammalian cell or an
avian cell.
284. A method of making a nucleic acid sequence comprising a sequence that encodes a 7
chain element (GC element) of a TCR y chain variable region (GCVR) and a 5 chain element (DC
element) of a TCR 5 chain variable region (DCVR), and n the GCVR and DCVR are matched,
comprising:
a) acquiring an isolated cell on site, e.g., a cell isolation micro-chamber, comprising:
i) a cell; and
ii) a capture ate capable of binding a first mRNA encoding a GCVR from the
cell and a second mRNA encoding a DCVR from the cell;
b) maintaining isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
n the isolated cell reaction site, e.g., cell isolation micro-chamber, does not include a
nucleic acid encoding a GCVR or a DCVR from a cell other than the cell;
c) contacting the mRNA loaded capture substrate with a reaction mixture, e.g., a reaction
mixture comprising reverse transcriptase, that uses the loaded mRNA as a template to make cDNA
(this can occur, e.g., in the ed cell reaction site, in the isolated production reaction site, or in
neither, e. g., not in an ed reaction site);
d) acquiring an isolated production reaction site, e.g., a production micro-chamber,
comprising:
WO 19402
i) an y chain (GC) strand from step b), wherein the GC strand is a strand of a 7 chain
double-stranded cDNA (GC ds cDNA) comprising a segment that encodes a GC element of
the GCVR from the cell, e.g., a 7 chain variable region sequence ); and
ii) a 5 chain (DC) strand from step b), wherein the DC strand is a strand of a 5 chain
double-stranded cDNA (DC ds cDNA) comprising a segment that encodes a DC element of
the DCVR from the cell, e.g., a 5 chain variable region sequence (DCVRS); and
e) covalent linking, e. g., ligation, of a GC strand to a DC ,
wherein the isolated production reaction site, e.g., a production micro-chamber, does not
include a nucleic acid encoding a DCVR or a GCVR from a cell other than the cell.
285. A method of making a nucleic acid sequence comprising a sequence that encodes a y
chain element (GC element) of a TCR 7 chain variable region (GCVR) and a 5 chain element (DC
element) of a TCR 5 chain variable region (DCVR), and wherein the GCVR and DCVR are matched,
comprising:
a) acquiring an ed cell reaction site, e.g., a cell isolation micro-chamber, comprising:
i) a cell; and
ii) a capture substrate e of binding a first mRNA ng a GCVR from the
cell and a second mRNA encoding a DCVR from the cell;
b) maintaining the isolated cell reaction site, e.g., the cell isolation micro-chamber, under
conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the
second mRNA to form the mRNA loaded capture substrate,
wherein the isolated cell reaction site, e.g., cell ion micro-chamber, does not include a
nucleic acid encoding a GCVR or a DCVR from a cell other than the cell;
c) ing an ed production reaction site, e.g., a production micro-chamber, comprises:
contacting the mRNA loaded capture substrate with a reaction e, e.g., a reaction mixture
comprising reverse transcriptase, that uses the loaded mRNA as a template, to produce:
a first double-stranded cDNA (ds cDNA) comprising a strand that is complementary to a first
mRNA that encodes a GCVR from a cell; and
a second ds cDNA comprising a strand complementary to a second mRNA encoding a DCVR
from the cell (the cDNA loaded e substrate);
wherein the isolated production on site, e.g., a production micro-chamber, does not
include a nucleic acid encoding a DCVR or a GCVR from a cell other than the cell.
d) maintaining the isolated production on site, e.g., the production micro-chamber,
under conditions that allow amplification of the first and second ds cDNAs, to produce:
a plurality of GC ds cDNAs comprising a segment that encodes a GC element of the GCVR
from the cell, e. g., a GCVRS; and
a plurality of DC ds cDNAs comprising a segment that encodes a DC element of the DCVR
from the cell, e.g., a DCVRS;
e) acquiring an ed linkage reaction site, e.g., a linkage micro-chamber, comprising:
covalent linking, e.g., ligation, of a strand of the GC ds cDNA (GC strand) to a strand of the DC ds
cDNA (DC ), n the GC and DC strands are both sense strands or antisense strands; and
f) amplifying the covalently linked, e.g., ligated, GC and DC s.
286. A method of making a library comprising a plurality of unique members, the method
comprising:
making the plurality of members, wherein each of the members comprises a sequence that
encodes a 7 chain element (GC element) of a 7 chain variable region (GCVR) and a 5 chain element
(DC element) of a 5 chain variable region (DCVR), and wherein the GCVR and DCVR are matched,
made by a method of any of paragraphs 201-285,
wherein each unique nucleic acid sequence of the plurality comprises a GC element and a DC
element from a different unique cell,
thereby making a library comprising a plurality of unique members.
287. The method of paragraph 86, wherein the plurality of unique s comprises at
least 204, 205, 206, 207, 208, or 209 unique members.
288. The method of paragraph 286 or 287, wherein the plurality of unique members
comprises 204 to 209, 204 to 208, 204 to 207, 204 to 206, 204 to 205, 208 to 209, 207 to 209, 206 to 209, 205
to 209, 205 to 208, 206 to 207, 204 to 205, 205 to 206, 206 to 207, 207 to 208, or 208 to 209 unique
289. The method of any of paragraphs 286-288, wherein at least 80%, 85%, 90%, 95%, 96%,
97%, 98%, 99%, or 200%, of the members in the library are unique members (which encode d
GC element and DC elements ces).
290. The method of any of paragraphs 286-289, wherein less than 20%, 25%, 20%, 5%, 4%,
3%, 2%, or 1%, of the members in the library are unique members (which encode matched GC
element and DC elements sequences).
291. A library comprising:
a plurality of unique members,
wherein,
i) each unique member of the plurality comprises a segment that encodes a GC element, e.g.,
a GCVRS, and a segment that encodes a DC element, e.g., a DCVRS, wherein the GC element and
the DC element in each unique member is matched;
ii) each unique member of the ity comprises a segment that s a GC element, e. g.,
a GCVRS, and a segment that encodes a DC element, e.g., a DCVRS, from a different unique cell;
iii) the library comprises one or more (e.g., two, three, four, or all) of the ing
properties:
a) the library is made by a method of any of paragraphs 201-285;
b) the plurality of unique s comprises at least 204, 205, 206, 207, 208, or 209
unique nucleic acid sequences;
c) the plurality of unique members comprises 204 to 209, 204 to 208, 204 to 207, 204 to
206, 204 to 205, 208 to 209, 207 to 209, 206 to 209, 205 to 209, 205 to 208, 206 to 207, 204 to 205,
205 to 206, 206 to 207, 207 to 208, or 208 to 209 unique s;
d) at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 200%, of the members in
the library are unique members (which encode matched GC element and DC elements
sequences); or
e) less than 20%, 25%, 20%, 5%, 4%, 3%, 2%, or 1%, of the members in the library
are unique s (which encode matched GC element and DC elements sequences).
292. The library of paragraph 291, wherein each unique member of the ity is
configured such that, when expressed, the GC element, e.g., the GCVRS, and the DC element, e.g.,
the DCVRS, form a functional antigen binding molecule, e.g., a single chain TCR.
293. The library of any of paragraphs 291-292, wherein the library is a display library.
294. The library of any of paragraphs 291-293, wherein each of the members of the plurality
further encodes a polypeptide that s in display of the member on the surface of a display entity.
295. The library of any of paragraphs 291-294, wherein the library is a phage display library.
296. The library of any of paragraphs 4, wherein the library is a yeast display library.
297. The library of any of paragraphs 291-294, n the library is a mammalian display
library.
298. A method of making a binding polypeptide, the method comprising:
a) acquiring a library of any of paragraphs 291-297; and
b) expressing a polypeptide encoded by a unique nucleic acid of the library.
299. The method of paragraph 298, further comprising contacting the polypeptide with an
antigen.
300. The method of paragraph 298 or 299, further comprising retrieving the nucleic acid that
encodes a ptide that binds the antigen.
301. An isolated tion on site, e.g., a production micro-chamber, which is an
isolated production reaction site described in any of paragraphs l-85, 101-185, or 5.
302. The isolated production reaction site, e.g., a production micro-chamber, of aph
401, which does not include a nucleic acid ng (i) an HCVR or an LCVR, (ii) an ACVR or a
BCVR, or (iii) a GCVR or a DCVR, from a cell other than the cell,
303. The isolated production reaction site, e.g., a production micro-chamber, of paragraph
301 or 302, which comprises one, two, or all of:
(i) one or more primers specific to V gene sequences of the (a) HC and LC, (b) a chain and [3
chain, or (c) 7 chain and 5 chain;
(ii) one or more primers specific to overhangs introduced onto the (a) HC and LC, (b) or chain
and [3 chain, or (c) 7 chain and 6 chain, cDNAs;
(iii) one or more primers comprising a first member, a second member, and a nucleotide
modification (e.g., a spacer) located between the first and second members, wherein the first member
is capable of annealing with the second member in the same primer or a ent primer, e. g., forming
a hairpin ure (via intramolecular hybridization) or a double-stranded structure (via
intermolecular hybridization) comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16,
17, 18, 19, 20, more basepairs.
304. The isolated production reaction site, e.g., a production micro-chamber, of any of
paragraphs 301-103, which does not comprise a reagent that can covalently link nucleic acids, e.g., a
ligase, e.g., a thermostable ligase.
305. The isolated production reaction site, e.g., a production micro-chamber, of any of
aphs 301-303, which comprises a reagent that can ntly link nucleic acids, e.g., a ligase,
e. g., a thermostable ligase.
306. A self-annealing oligonucleotide comprising a first member, a second member, and a
nucleotide modification (e.g., a ) located between the first and second members, wherein the
first member is capable of annealing with the second member in the same oligonucleotide or a
different oligonucleotide, e. g., forming a hairpin structure (via intramolecular hybridization) or a
-stranded structure (via intermolecular hybridization) comprising a duplex region of 4, 5, 6, 7,
8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more basepairs.
307. The ucleotide of paragraph 306, wherein the first and second s are
capable of forming a hairpin structure comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,
, 16, 17, 18, 19,20, more basepairs.
308. The oligonucleotide of paragraph 306 or 307, wherein the first member is 5-40
nucleotides, e.g., 5-10, 5-20, 5-30, 30-40, 20-40, 10-30, 10-30, or 15-25 nucleotides, in length.
309. The oligonucleotide of any of paragraphs 306-308, wherein the second member is 5-40
nucleotides, e.g., 5-10, 5-20, 5-30, 30-40, 20-40, 10-30, 10-30, or 15-25 nucleotides, in .
310. The oligonucleotide of any of paragraphs 306-309, n the spacer is a flexible
spacer or a PEG spacer.
311. The oligonucleotide of any of paragraphs 0, wherein the first member comprises
a sequence that is complementary to the sequence of an oligonucleotide attached to a e
substrate.
312. The ucleotide of any of paragraphs 306-311, wherein the second member
comprises (e.g., from 5’ to 3’) one, two, or all of: (i) a sequence that is complementary to at least a
portion of the first member; (ii) a universal priming sequence (e.g., for PCR amplification or next-
generation sequencing); and (iii) a sequence mentary to a target sequence, e.g., a GCVRS
andfor a DCVRS, ally, wherein the second member comprises a sequence for homologous
recombination (e.g., in a yeast or ian cell).
313. An isolated linkage reaction site, e. g., a linkage micro-chamber, which is an isolated
linkage reaction site described in any of paragraphs 51-83, 85, 151-183, 185, 251-283, or 285.
314. The ed linkage reaction site, e.g., a linkage micro-chamber, of paragraph 313,
which does not include a nucleic acid encoding (i) an HCVR or an LCVR, (ii) an ACVR or a BCVR,
or (iii) a GCVR or a DCVR, from a cell other than the cell,
315. The isolated linkage reaction site, e.g., a linkage micro-chamber, of paragraph 313 or
314, which comprises a splint oligonucleotide that is capable of hybridizing to a sequence comprising
the junction of (i) the HC strand and the LC strand, (ii) the AC strand and the BC strand, or (iii) the
GC strand and the DC strand, or a sequence complementary thereof, to form a duplexed region at the
site of ligation.
316. The isolated linkage reaction site, e.g., a linkage micro-chamber, of any of paragraphs
313-315, which ses a reagent that can covalently link nucleic acids, e.g., a , e.g., a
thermostable ligase.
317. The method of any of paragraphs 1-300, which does not include a step of overlap
extension polymerase chain reaction (OE-PCR), also known as splicing by overlap extension or
splicing by overhang extension (SOE) PCR (Higuchi er al., Nucleic Acids Res. 1988; 16(15):7351-
67), e.g., in the linking step.
EXAMPLES
Example 1: Cohesive-End PCR-Ligation in Drops
B cells were encapsulated in ts. B cells were encapsulated into droplets, with droplet
volume ranging from 10 pL to 100 nL, typically 000 pL. s of B cells can e, for
example, mice, human, rat, rabbit, or chicken. The carrier (oil) phase was composed of 3M HFE-
7500 with ~1% urfactant (RAN Biotechnologies). Droplets were formed by a microfluidic
chip (e.g., 2R 100 pm from Dolomite) with flow of fluid phases controlled by a syringe or pressure
pump. The aqueous phase of droplets was composed of a buffer (e.g., Tris, pH 7.5), a detergent to aid
cell lysis, and magnetic beads which contain oligonucleotides (primers) to anneal to heavy and light
chain mRNAs. Occupancy of drops should be not more than 1 cell per droplet, and at least one bead
per droplet.
The droplets were incubated to facilitate cell lysis. The on was heated to improved
lysis efficiency in the ce of certain detergents (e.g., Tween 20). For e, the emulsion can
be heated to a temperature of 40°C, 50°C, 60°C, 70°C, or 80°C, for approximately 5-60 minutes.
After the cells were lysed, mRNA was released and captured on beads by annealing to
oligonucleotides on the beads.
The on was broken and the beads were red. Emulsions (or coalesce different
solution phases) were broken using drop destabilizing reagent, such as PFO (perfluorooctanol). The
aqueous phase containing the beads was recovered. The beads were isolated using magnet. The
beads were washed and resuspended in a buffer (e.g., Tris, pH 7.5).
Reverse transcription (RT) was performed to create cDNA-beads (in a non-emulsion
reaction). The beads were resuspended in a buffer-enzyme mix to facilitate RT (e.g., Superscript II
RT). The reaction was incubated at 40°C for 15 minutes to facilitate RT. The beads were washed
once with a buffer (6.3., Tris, pH 7.5).
The recovered beads can be ulated for PCR-Ligation reaction. Droplets g in
volume from about 5 pL-500 pL, most commonly about 10-50 pL, can be used. The carrier (oil)
phase can be composed of 3M HFE-7500 containing ~2% fluorosurfactant (RAN hnologies).
Drops can be encapsulated with: beads which have cDNA; PCR reagents (including, e.g., a DNA
polymerase, e.g., Phusion® High-Fidelity DNA Polymerase (NEB), Q5® High-Fidelity DNA
Polymerase (NEB), Pfu DNA rase, KAPA DNA polymerase, Vent® DNA polymerase, or Taq
DNA polymerase); ucleotides that allow for amplification of VH and VL sequences; a
thermostable ligase (e.g., Taq DNA ligase, Pfu DNA ligase, Ampligase® thermostable DNA ligase,
Tsc DNA ligase, Rma DNA ligase, Tfi DNA ligase, or Tth DNA ligase); and optimized buffer
conditions (compatibility to support both DNA polymerase and ligase enzymatic activities).
The scFV cassette was constructed as VL-Linker-VH, as tested in tubes. The order can be
switched (VH-Linker-VL) with no cant impact on function or performance. The e
s of the VL sequence can contain an overhang sequence encoding a Linker sequence and with
at least 1 modified nucleotide (e.g., 3 consecutive nucleotides with 2’-O-methyl modification). The
VL reverse primers can also contain 5’-phosphate (required to be substrate of ligase). The forward
primers of the VH sequence can contain an overhang sequence encoding a Linker sequence and with
at least 1 modified nucleotide (e.g., 3 consecutive nucleotides with 2’-O-methyl modification). The
VH forward primers can also contain 5’-phosphate red to be substrate of ligase). The
occupancy of drops should be not more than 1 bead per drop.
Thermocycling can be med with emulsion. The ted emulsion can be transferred
to PCR tubes. Thermocycling can be performed using the following conditions: initial denaturation at
95-98°C for 30 s to 2 s; 10-30 cycles of: denaturation at 95-98°C for 10-30 seconds,
primer annealing at 50-60°C for 10-30 seconds, polymerase extension at 72°C for 30 seconds, and
ve product annealing and ligation at 45-55°C for 3 minutes. The reaction can be hold at 4°C.
The emulsion was broken and the portion which contains linked (and non-linked) t was
recovered. Emulsions can be broken using drop destabilizing reagent, such as PFO
(perfluorooctanol). The s phase can be recovered and the beads can be discarded.
The linked product (representing natively linked VL—linker-VH), as tested in tubes, was
purified from non-linked VH and VL products. Ligated product was separated from non-ligated
products by size separation. For example, denaturing PAGE (polyacrylamide gel electrophoresis) or
denaturing HPLC-SEC can be used. Linked product (about 800-900 bp) was isolated from non-linked
product (about 350-500 bp). For denaturing PAGE purification, the ligated band was cut out from the
gel and electroelution was performed to extract DNA from the gel slice (Bio-Rad Electro-Elutor).
The purified linked product can be amplified by PCR. Polymerase/conditions which can
moderately read through DNA ning modified nucleotides, e.g., Taq polymerase, can be used.
Final PCR product can be introduced to yeast using standard methods (e.g., oporation
with expression vector) to create a natively paired library derived from biological sources.
Example 2: Ligase Cycling Approach
B cells were encapsulated in droplets. B cells were encapsulated into droplets, with droplet
volume ranging from 10 pL to 100 nL, typically 000 pL. Sources of B cells can include, for
example, mice, human, rat, rabbit, or chicken. The carrier (oil) phase was composed of 3M HFE-
7500 with ~1% fluorosurfactant (RAN Biotechnologies). Droplets were formed by a microfluidic
chip (e.g., 2R 100 pm from Dolomite) with flow of fluid phases controlled by a syringe or pressure
pump. The aqueous phase of droplets was composed of a buffer (e.g., Tris, pH 7.5), a detergent to aid
cell lysis, and magnetic beads which contain ucleotides (primers) to anneal to heavy and light
chain mRNAs. Occupancy of drops should be not more than 1 cell per droplet, and at least one bead
per droplet.
The droplets were incubated to facilitate cell lysis. The on was heated to improved
lysis efficiency in the presence of certain detergents (e.g., Tween 20). For example, the emulsion can
be heated to a temperature of 40°C, 50°C, 60°C, 70°C, or 80°C, for approximately 5-60 minutes.
After the cells were lysed, mRNA was released and captured on beads by annealing to
ucleotides on the beads.
The emulsion was broken and the beads were recovered. Emulsions (or coalesce different
solution phases) were broken using drop destabilizing reagent, such as PFO (perfluorooctanol). The
aqueous phase ning the beads was recovered. The beads were isolated using magnet. The
beads were washed and resuspended in a buffer (6.3., Tris, pH 7.5).
Reverse transcription (RT) can be performed to create eads (in a non-emulsion
reaction). The beads can be resuspended in a -enzyme mix to facilitate RT (e.g., Superscript 11
RT). The reaction can be ted at 40°C for 15 minutes to facilitate RT. The beads can be washed
once with a buffer (e.g., Tris, pH 7.5).
Recovered beads were encapsulated for PCR reaction. Droplets ranging in volume from
about 5 pL—500 pL, most commonly about 10-50 pL, can be used. The carrier (oil) phase can be
composed of 3M 00 ning ~2% fluorosurfactant (RAN Biotechnologies). Drops can be
encapsulated with: beads which contain cDNA (some beads can be ‘empty’, which is not
problematic); PCR ts (including, e.g., a DNA polymerase, e.g., n® High-Fidelity DNA
rase (NEB), Q5® idelity DNA Polymerase (NEB), Pfu DNA rase, KAPA DNA
polymerase, Vent® DNA polymerase, or Taq DNA polymerase); oligonucleotides that allow for
amplification of VH and VL sequences.
The scFv cassette can be constructed as VL—Linker-VH. The order can be switched (VH-
Linker-VL) with no significant impact on function or performance. The VL reverse and VH d
primers can contain an overhang sequence encoding Linker. The VH e primer can have a 5’-
phosphate. The occupancy of drops should be not more than 1 bead per drop.
Thermocycling can be performed with on. The generated emulsion can be erred
to PCR tubes. The thermocycling can be performed using standard PCR conditions: initial
denaturation: 95-98°C for 30 seconds to 2 minutes; 10-30 cycles of: denaturation at 95-98°C for 10-
seconds, primer annealing at 50-60°C for 10-30 seconds, polymerase extension at 72°C for 30
seconds. The reaction can be hold at 4°C.
The on can be broken and the beads can be recovered. Emulsions (or coalesce
different solution phases) can be broken using drop destabilizing reagent, such as PFO
(perfluorooctanol). The aqueous phase that contains beads can be recovered. The beads can be
isolated using magnet. The beads can be washed to remove PCR product not captured on beads. The
beads can be resuspended in a buffer (e.g., Tris, pH 7.5).
The recovered beads can be ulated for ligase cycling reaction. Droplets g in
volume from about 5 pL-SOO pL, most ly about 10-50 pL, can be used. The carrier (oil)
phase can be composed of 3M HFE-7500 containing ~2% fiuorosurfactant (RAN Biotechnologies).
The drops can be encapsulated with a Stint oligonucleotide which is complementary and anneals to 3’
terminus of ‘top’ VL strain and 5’ terminus of ‘top’ VH strand; a thermostable ligase (e.g., Taq DNA
ligase, Pfu DNA ligase, Ampligase® thermostable DNA ligase, Tsc DNA ligase, Rma DNA ligase,
Tfi DNA ligase, or Tth DNA ligase); reaction ents ed to support ligase enzymatic
activity (e.g., NAD). The occupancy should be not more than 1 bead per drop.
Thermocycling can be performed with emulsion. The ted emulsion can be transferred
to PCR tubes. Thermocycling can be performed using rd conditions: 3-15 cycles of:
denaturation: 90-95°C for 30 seconds, annealing and ligation at 50-60°C for 1-3 minutes. The
reaction can be hold at 4°C.
The emulsion can be broken and the aqueous portion which contains the linked product can
be recovered. Emulsions (or coalesce different solution phases) can be broken using drop
destabilizing reagent, such as PFO (perfluorooctanol). The aqueous phase can be recovered and the
beads can be discarded.
The purified linked product can be amplified by PCR. Standard conditions can be used with
oligonucleotides that anneal the outer termini of the linked VL-Linker-VH d fragment.
Final PCR product can be introduced to yeast to create a natively paired library derived from
biological sources.
Example 3: Ligase Cycling Reaction
In this example, VH and VL PCR products were covalently linked using a thermostable ligase
and a splint oligo.
RNA from a hybridoma clone (ATCC, HB-112, “4G2”) was isolated using an RNeasy Kit
n). cDNA was generated using SuperScript IV Reverse Transcriptase (Thermo Fisher) and the
isolated RNA ing manufacturer recommended conditions. Primers used were directed to
nt regions of the heavy and light chains: mouse IgG_CHl_rev (5’-
CHGATGGGGSTGTYGTTKTRGC (SEQ ID NO: 1)) and mouse Kappa_Rev (5 ’-
GTGCAGCATCAGCCC (SEQ ID NO: 2)). As used herein, the nucleotides are defined by IUPAC
nucleotide code, e.g., R=A or G; Y=C or T; S: G or C; K=G or T; H=A or C or T.
The components were mixed, heated to 65 °C for 5 min, and then incubated on ice for at least
1 min. The following components were then added:
Component Volume (111)
5X SSIV buffer 4
100mm
RNaseOUT RNase Inhibitor (40 U/ul)
SuperScript IV Reverse Transcriptase
The components were mixed and then incubated at 55°C for 10 minutes. The reaction was
then inactivated by incubating at 80°C for 10 minutes. cDNA ts were used as templates for
PCR to separately generate VH and VL products. The s used ed:
4G2 VL_Fwd
5’-GACATCAAGATGACCCAGTCTC (SEQ ID NO: 3)
Mouse Kappa_Rev-Phos
’-/5Phos/ACCAGCAGAGCTCTCACCTGGTGCAGCATCAGCCC (SEQ ID NO: 4)
4G2 VH_Fwd-Phos
’-/5Phos/GGAACTACCGAAGGCACAGGTGAGGTCCAGCTGCAACAGTC (SEQ ID NO: 5)
Mouse IgG_CHl_rev
’-CHGATGGGGSTGTYGTTKTRGC (SEQ ID NO: 1)
WO 19402
The PCR reactions each included:
Q5 Hot Start 2X Master Mix (NEB)
Thermocycling was then performed as follows:
Initial
Denature Anneal Extend
Denaturation
Forever
PCR products were purified using AMPure beads. Purified PCR ts were quantified by
op. Ligase cycling reactions were set up in 25 ul reactions to achieve a 1:1:1 molar ratio of
VH product, VL product, and splint oligo. The splint oligo used had the following sequence:
’—ACCTGTGCCTTCGGTAGTTCCACCAGCAGAGCTCTCACCTG/3AmMO/ (SEQ ID NO: 6).
Each ligase cycling reaction included:
Component Amount
4G2 VH PCR product 38 ng
4G2 VL PCR t 35 ng
Splint oligo (0.1 mM) 3.3 ul
Taq ligase buffer (NEB) 2.5 ul
Taq ligase (NEB) 1 ul
Water Up to 25 ul
The ligase cycling reactions were analyzed by denaturing PAGE (polyacrylamide gel
electrophoresis). In addition to Taq Ligase, an onal thermostable ligase (Ampligase (Amp),
Lucigen) was evaluated.
As shown in efficient linking of VH and VL product was observed using both Taq
ligase and Ampligase thermostable ligase.
Example 4: Retention of Native g During Bulk Re-Amplification of Linked Products
An emulsion OE-PCR workflow creates VH and VL products which arily share
ces to facilitate splicing by p extension. In such a w, any retained VH/VL
product which is not spliced together within a drop can become spliced during bulk (non-emulsion)
PCR amplification of products. Since such splicing occurs during the bulk phase when all cell
products (e.g., many clones/unique sequences) are mixed, random (non-native) pairing could occur
(see Eur J Immunol. 2013; 43(9):2507-15). The workflow would benefit from a strategy that not only
reduces this occurrence, but completely prevents it. Described herein is a method in which VH and
VL products do not share any common sequence, and thus the workflow is not susceptible to
incidental splicing by OE-PCR during bulk re-amplification steps.
To demonstrate this, linked VH-VL ts were generated from two hybridoma clones
which differ from each other by size. Native versus non-native pairing could therefore be assessed by
size of products. VH and VL products from hybridoma 4G2 were generated as described in Example
3. Hybridoma 9E10 (ATCC, CRL-l729) “mini” VH and VL products were similarly produced using
the same RT primers and the following PCR primers:
9E10 Mini_VL_Fwd
’-GGCAGTGGGTCTGGGACAG (SEQ ID NO: 7)
mouse Kappa_ReV-Phos
5’-/5Phos/ACCAGCAGAGCTCTCACCTGGTGCAGCATCAGCCC (SEQ ID NO: 4)
9E10_VH_Fwd
’ -/5Phos/GGAACTACCGAAGGCACAGGTGAGGTGCACCTGGTGGAGTCTGGGGG (SEQ ID
NO: 8)
9E 10 Mini_VH_ReV
5’ —GGATAGTGGGTGTAAGTACCACGACTACCAATG (SEQ ID NO: 9)
The 4G2 VH and VL products produced were of size 400 bp and 378 bp, respectively. The
mini 9E10 VH and VL products produced were of size 203 bp and 168 bp, tively. The PCR
products were purified using AMPure beads.
The products were then subjected to ligase cycling reactions as described in Example 3, using
cycles of thermocycling. 4G2 and 9E10 VH-VL products were ligated in separate tubes, simulating
tion of natively linked products in droplets (in individual compartments). The products were
analyzed by denaturing PAGE. Natively linked products of 4G2 VH-VL and of 9E10 VH-VL
corresponded to sizes of 778 bp and 371 bp, respectively. Twenty cycles of ligase g were used
to create products ning both ligated ts as well as non-ligated VH and VL DNA. As
shown in , natively linked VH-VL products were generated for both 6G2 and 9E10.
The ligation products were purified by AMPure beads and then combined and used as
te for PCR re-amplification of linked products. This step simulated recovery of products after
ming linking of VH-VL in drops, ed by PCR re-amplification of linked VH—VL. By
having non-ligated VH and VL DNA as template in the PCR, this provided an opportunity for DNA
to become linked together during bulk re-amplification by PCR. Since the DNA was not
compartmentalized by individual clones, any linkng that occurred in bulk phase would lead to non-
native pairing.
PCR re-amplification was performed as described below. ” primers used to amplify
linked products are as follows:
9E10 Mini_VL_Fwd
’-GGCAGTGGGTCTGGGACAG (SEQ ID NO: 7)
4G2 VL_Fwd
ATCAAGATGACCCAGTCTC (SEQ ID NO: 3)
9E 10 Mini_VH_Rev
5’-GGATAGTGGGTGTAAGTACCACGACTACCAATG (SEQ ID NO: 9)
Mouse IgG_CHl_rev
’-CHGATGGGGSTGTYGTTKTRGC (SEQ ID NO: 1)
The PCR reactions each included:
Q5 Hot Start 2X Master Mix
Fwd primers (10 uM) 0.9 (each)
Rev primers (10 uM) 0.9 (each)
Purified ligation products 4.5 (each)
Thermocycling was then performed as follows:
Initial
Denature Anneal Extend
ration
Finally, ts were analyzed by agarose gel electrophoresis. Non-natively linked products
could be readily identified by their intermediate size. Specifically, 4G2-VL / 9E10-mini-VH and
9E10-mini-VL / 4G2 VH linked products would correspond to sizes of 581 bp and 568 bp,
respectively. As shown in , ion of native pairing was observed when mixed during bulk
re-amplification (lane 5).
Example 5: Oligo Design to Facilitate Capture of PCR Product onto Beads
After RT-PCR in droplets (e.g., as described above), the PCR product can be captured onto
beads within the drop to retain native matching of the VH and VL products. To facilitate efficient
capture of dsDNA PCR ts onto beads, a strategy was devised to te products which have
defined ssDNA at their ends. The sequence of this ssDNA is complementary to sequence of an oligo
conjugated to the beads. The complementarity of these sequences thus facilitates specific and
efficient capture of dsDNA PCR products.
PCR products from 4G2 cDNA were produced using PCR with the following primers and Q5
DNA polymerase. The reverse primer contains a 5’-biotin moiety to facilitate specific detection of
PCR product on beads. The PCR gy was as follows:
(1) (VL) 4G2 VL Fwd +PEG Mus Kap Rev biotin
(2) (VL) 4G2 VL Fwd —PEG Mus Kap Rev biotin
(3) (VL) 4G2 VL Fwd No_5’ Mus Kap Rev biotin
(3) (VH) 4G2 VH Fwd Biotin Mus IgG-HC Rev +PEG
The ing primers were used:
4G2 VL Fwd +PEG
TGGATCGTTACTAATATTCGC/iSp18/GGACTCAGACACTTCCGTGCGACATCAAGATGACC
CAGTCTC (SEQ ID NO: 10)
4G2 VL Fwd -PEG
GTTACTAATATTCGCGGACTCAGACACTTCCGTGCGACATCAAGATGACCCAGT
CTC (SEQ ID NO: 11)
4G2 VL Fwd No_5’
5’-GGACTCAGACACTTCCGTGCGACATCAAGATGACCCAGTCTC (SEQ ID NO: 12)
Mus Kap Rev biotin
’-/5BiotinTEG/ACCAGCAGAGCTCTCACCTGGTGCAGCATCAGCCC (SEQ ID NO: 13)
Mus IgG-HC Rev +PEG
GCAATCCATCAACGTC/iSp l8/CGTGACACATGTGGTTCAAGTACGGCHGATGGGGSTGTY
GTTKTRGC (SEQ ID NO: 14)
4G2 VH Fwd Biotin
’-/5BiotinTEG/GGAACTACCGAAGGCACAGGTGAGGTCCAGCTGCAACAGTC (SEQ ID NO:
PCR products were analyzed by agarose gel electrophoresis, ed by AMPure beads and
fied by Nanodrop.
Amine-labeled capture oligos were conjugated to carboxylated beads L, Bangs
Laboratories) using standard methods. The following oligos having sequence complementary to
sequence 5’ of the PEG s in the above PCR primers were used for conjugation to beads. Beads
were conjugated to VL oligo only, to VH oligo only, or to both oligos.
VL Capture
GCGAATATTAGTAACGATCCAAAAAAAAAAAAAAAAAAAAA/iSp 18/iiSp1 8//iSp18//3AmM
O/ (SEQ ID NO: 16)
’ -GACGTTGATGGATTGCAAAAAAAAAAAAAAAAAAAA/iSp18//iSp18//iSp18//3AmMO/
(SEQ ID NO: 17)
To capture PCR product, the following reactions were setup. Purified PCR products were
diluted in 0.5X SSC buffer to achieve 250 ng DNA in 25 ul final volume. Oligo-conjugated beads
were washed with 0.5X SSC, and 400,000 beads were then mixed with each diluted PCR product. The
bead-PCR product mix was mixed by pipet to suspend the beads, and the tubes were then placed in a
thermocycler and the following program was run:
Step Temp Time
1 70 0C 30 sec
2 55 0C 30 sec
3 50 0C 30 sec
4 45 °C 4 min
40 OC 4 min
6 35 °C 4 min
7 4 OC hold
The samples were as follows:
Blank (no capture oligo) (1) (VL)
2 Blank (no capture oligo) (2) (VL)
3 Blank (no capture oligo) (3) (VL)
4 Blank (no capture oligo) (4) (VH)
VL capture (1) (VL)
6 VL capture (2) (VL)
7 VL capture (3) (VL)
8 VL capture (4) (VH)
9 VH e (1) (VL)
VH capture (4) (VH)
11 VH and VL capture (1) (VL)
12 VH and VL capture (4) (VH)
After temperature incubation, the samples were applied to a magnet to collect beads on the
side of the tube. Supernatants were removed, and the beads were washed with 0.5X SSC buffer.
Each sample was probed for captured PCR product with 50 pl of AlexaFluor 647 IgG Fraction
Monoclonal Mouse Anti-Biotin antibody (Jackson) diluted 1:1000 in PBS containing 1% BSA. After
incubation with mixing for 25 minutes at room temperature, the tubes were applied to a magnet, the
supernatants were removed, and the beads were washed with PBS buffer. The beads were
resuspended in 50 ul PBS and then analyzed by flow cytometry.
As shown in FIGS. 5A-SB, the results demonstrate ent and specific PCR product
capture by this method, with a requirement for a PEG spacer in the PCR s and an riate
complementary oligo conjugated to the beads (e. g., as in sample (1)).
Example 6: Generation of Natively Paired VH-VL Product in Drops by Ligase g
In this example, cells expressing VH and VL sequences of antibody 4G2 were lysed and
mRNAs from the lysate were used to generate natively paired VH-VL products by ligase cycling.
Cell lysis buffer was prepared as follows:
300 ul 20% Ficoll PM400
ul 20% Sarkosyl
40 ul 5 mM EDTA
100 ul 2M Tris pH 7.5
350 ul Water
200 ul 100% ep
Carboxylated COMPEL magnetic beads (Bangs Labs) were conjugated with the ing
amine-modified oligos:
VL Capture
GCGAATATTAGTAACGATCCAAAAAAAAAAAAAAAAAAAAA/iSp 18//iSp1 8//iSp18//3AmM
O/ (SEQ ID NO: 16)
’ TGATGGATTGCAAAAAAAAAAAAAAAAAAAA/iSp18//iSp18;’/iSp18//3AmMO/
(SEQ ID NO: 17)
Mus_IgG_CH1_mRNA capture
CTGGACAGGGATCCAKAGTTCCAAAAAAAAAAAAAAAAAAAAA/iSp18//iSp18//iSp18//3A
mMO/ (SEQ ID NO: 18)
Mus_Kap_mRNA capture
’ -GTGCAGCATCAGCCCGAAAAAAAAAAAAAAAAAAAA/iSp18//iSp18i/iSp18/l3AmMO/
(SEQ ID NO: 19)
Oligo(dT) may also be used for mRNA capture (e.g., for capture of mRNAs encoding a VH
andfor VL).
Beads were suspended in lysis buffer at a density of 2x107 beads/ml, which tates
encapsulation of about 2.5 beads per drop with drop sizes used.
4G2 mouse hybridoma cells were washed with PBS and resuspended in PBS containing 0.1%
BSA and 24% Optiprep at a density of 2x106 cells/m1. This density facilitates encapsulation into drops
at approximately one cell per two drops. For co-encapsulation of cells and beads in drops, a 2-
reagent, 100 pm diameter fluorophilic microfluidic chip (Dolomite) was used with solution flow
controlled by three Mitos P-Pump pressure pumps (Dolomite). The microfluidic chip contained two
input channels for s solutions and two input channels for fluorocarbon oil. Cells and beads
were flowed at a rate of 10 til/min each, and fluorinated oil (HFE-7500 containing 1%
urfactant (RAN Biotechnologies)) was flowed at 55 til/min. These flow rates resulted in
ts of approximately 500 pl in volume. ons were collected for an hour.
The emulsions (cells and no-cell control) were applied to a heat block at 45 0C for 20 min to
facilitate mRNA capture onto beads. The emulsions and beads were subsequently kept and handled at
4°C to reduce dissociation of mRNA from beads. Excess oil was removed by syringe, and 5 ml of
ice-cold 6X SSC buffer containing RNAse inhibitor (1:100) was added to the emulsion. PFO
(1H,1H,2H,2H-Perfluorooctanol) was added (100 ML) to the emulsion to induce drop coalescence.
The sample was mixed ghly by inversion followed by fugation at 500xg for 5 min at 4°C.
The aqueous phase was mixed by pipet, collected and then transferred to a new tube. After applying
the tube to the magnet, the beads (having hybridized mRNA) were washed twice with 500 ul of ice-
cold buffer (100 mM Tris HCl pH 8, 500 mM KCl, 15 mM MgC12) containing RNase inhibitor
(1 : 100). The beads were then ded in 500 u] of ice-cold buffer (100 mM Tris HCl pH 8, 500
mM KCl, 15 mM MgC12) containing RNase inhibitor (1:100).
A second emulsion was prepared with the mRNA beads and RT-PCR mix. The mRNA beads
were suspended in the RT-PCR bead buffer (100 ul 2X RT—PCR buffer (OneTaq ep RT-PCR,
NEB), 70 ul of ep, 4 ul of RNase inhibitor, 6.4 ul of premixed 25 uM s, and 32 ul of
water). Primers used were:
4G2 VL Fwd +PEG
TGGATCGTTACTAATATTCGC/iSp18/GGACTCAGACACTTCCGTGCGACATCAAGATGACC
CAGTCTC (SEQ ID NO: 10)
Mus IgG-HC Rev +PEG
GCAATCCATCAACGTC/iSp18/CGTGACACATGTGGTTCAAGTACGGCHGATGGGGSTGTY
GTTKTRGC (SEQ ID NO: 14)
mouse Kappa_Rev-Phos
’—/5Phos/ACCAGCAGAGCTCTCACCTGGTGCAGCATCAGCCC (SEQ ID NO: 4)
4G2 VH Fwd-Phos
’-/5Phos/GGAACTACCGAAGGCACAGGTGAGGTCCAGCTGCAACAGTC (SEQ ID NO: 5)
Separately, enzyme mix was prepared (400 ul 2X RT-PCR buffer, 43 ul of enzyme diluted to
1.33X in RT-PCR buffer, 16 ul RNase inhibitor, and 341 ul water).
ons for RT-PCR were generated by co-flowing beads and enzyme mix at 2.5 til/min
and 7.5 ul/min, respectively, and oil (HFE7500 containing 2% fluorosurfactant) at 35 ul/min. A two-
reagent 30 um diameter fluorophilic microfluidic chip (Dolomite) was used with pressure pumps to
generate droplets. Under these conditions, droplets of approximately 15-35 pl in volume were
, with bead occupancy of less than one bead per 5 drops. Drops were generated until all beads
were encapsulated (about 30-60 minutes). The emulsions were then aliquoted into PCR tubes for
thermocyling in a thermocycler with the following program:
Denature
1 Cycle
55°C 50°C 45°C 40°C 12°C
30 4 min Forever
sec sec
The thermocycled emulsions were combined into one tube. Excess oil was removed from the
bottom using a syringe. To break the drops, 100 ul of PFO was added followed by mixing and
centrifugation at 2000xg for two minutes. The aqueous phase was then mixed by pipet (to suspend
beads) and transferred to a new tube. The beads contained captured PCR product. The tube was
d to a magnet and supernatant removed. The beads were gently washed with 500 pl of ice-cold
0.5X SSC buffer. The beads were again applied to a magnet, supernatant d and then
resuspnded in 100 pl ice-cold 0.5X SSC buffer.
Samples were then prepared for ligase cycling emulsion to covalently link VH and VL
products. The beads were ded in bead on (20 ul 10X Taq Ligase buffer (NEB), 117 pl
60% sucrose, and 63 ul water). Taq ligase mixture was prepared (80 ul 10X Taq Ligase buffer, 42 ul
Taq Ligase, 13 ul of splint oligo (at 0.1 uM), and 665 pl water). The splint oligo used was
’ TGCCTTCGGTAGTTCCACCAGCAGAGCTCTCACCTG/3AmMO/ (SEQ ID NO: 6).
Emulsions were generated by flowing the bead solution at 2 ul/min, enzyme mix at 6 ul/min,
and oil (HFE7500 containing 2% urfactant) at 35 ul/min. A two-reagent 30 um diameter
fluorophilic microfluidic chip (Dolomite) was used with pressure pumps to generate droplets. Under
these conditions, droplets of approximately 15-35 pl in volume were formed, with bead occupancy of
less than one bead per 5 drops. Drops were generated until all beads were encapsulated (about 30-60
minutes). The emulsion was then aliquoted into PCR tubes for thermocyling in a thermocycler using
the following program:
Initial Denature Anneal
Denaturation
1 cycle 40 cycles
2 minutes 20 30 1 Forever
seconds seconds minute
The thermocycled emulsions were combined into one tube. Excess oil was removed from the
bottom using a e. To break drops, 100 pl of PFO was added ed by mixing and
centrifugation at 2000xg for two minutes. The aqueous phase was then mixed by pipet (to suspend
beads) and transferred to PCR tubes (50 ul per tube). The samples were then applied to a thermocyler
preheated to 80°C to facilitate dissociation of d PCR ts hybridized to beads. After
allowing beads to , the supernatant was removed and transferred to a new tube. The products
were then purified using AMPure beads.
The ligated products were then reamplified in tubes by PCR using the following primers:
4G2-VL—Fwd-Reamp 5’—TGACCCAGTCTCCATCTTCA (SEQ ID NO: 23)
4G2-VH-Rev-Reamp 5’—TGTTGTTTTGGCTGAGGAGA (SEQ ID NO: 24)
Component Volume (111)
Thermocycling was performed as follows:
Initial
Denature Anneal Extend
Denaturation
The reamplification products were analyzed by agarose gel electrophoresis. As shown in the 4G2 cell sample yielded the intended product of linked VH-VL DNA, whereas negative control
samples did not yield any product.
It is contemplated that the final PCR t, containing, e.g., natively paired VL and VH
with a flexible linker sequence between in an intact open reading frame, can be cloned into a yeast
surface expression vector and transformed into yeast using standard methods, resulting in a natively
paired yeast y library. For example, an additional 50 bp on each terminus can be incorporated
by PCR. These 50 bp match appropriate sequences in a yeast expression vector to facilitate cloning
by homologous recombination in yeast, following yeast co-transformation of insert PCR product (e.g.,
containing ly linked VL-VH) and a linearized yeast expression vector.
e 7: Self-Annealing Primers to Prevent PCR Product Capture ition from Primers
PCR product capture onto beads was facilitated by tion of sticky ends (ssDNA) on a
terminus of PCR products through incorporation of a spacer, such as PEG. These ssDNA portions
had ce complementarity to oligos conjugated to beads, thereby tating specific
hybridization. The sequences in the PCR products were incorporated by their inclusion in
amplification primers. Since the PCR primers had the same sequences as the PCR product ssDNA
termini, they too can be captured onto beads through specific hybridization. Such primer capture
would necessarily e with PCR product capture.
Amplification of antibody repertoires by PCR typically requires a multitude of PCR s
to amplify the varied antibody gene sequences. For example, primer sets recognizing V and J regions
of human and mouse repertoires are often ed of about 15-20 s directed to the V regions
of the heavy and light chains. The requirement for a multitude of primers leads to circumstances that
exacerbate the competing effect of s on capturing PCR t onto beads. For multiplex PCR
scenarios, in a given drop (compartment), a single primer (or limited number) can have
complementarity to the target dy sequence within in the drop. The remaining repertoire primers
will not match the target sequence. These non-matching primers would not be incorporated into
amplicons during PCR, and as such, are poised to effectively compete for PCR product capture. To
s this limitation, a design was ted that mitigates the ability of the unused PCR primers
within a drop to compete with PCR product for capture. PCR product capture can be able, for
example, under conditions of 15:1 molar ratio of unused primer to primer that generates amplicon.
Carboxylated magnetic beads were conjugated with the following amine-modified oligos, as
described above.
VL_Capture
GCGAATATTAGTAACGATCCAAAAAAAAAAAAAAAAAAAAA/iSp l8//iSp l 8//iSp l8//3AmM
O/ (SEQ ID NO: 16)
VH_Capture
GACGTTGATGGATTGCAAAAAAAAAAAAAAAAAAAA/iSp lSli’iSp l8//iSp18//3AmMO/ (SEQ
ID NO: 17)
Mus_IgG_CH1_mRNA_capt
AGGGATCCAKAGTTCCAAAAAAAAAAAAAAAAAAAAA/iSp18//iSpl8//iSpl8//3A
mMO/ (SEQ ID NO: 18)
p_mRNA_capt
GTGCAGCATCAGCCCGAAAAAAAAAAAAAAAAAAAA/iSp18/!iSp18//iSpl8//3AmMO/ (SEQ
ID NO: 19)
RT-PCR reactions were set up to amplify VL sequence corresponding to 4G2 hybridoma
using the following oligos. These oligos represent a design without a self-annealing ce (Non-
SA) and a design with a self-annealing sequence (SA). Additionally, an oligo that has a self-annealing
’ -end but has
sequence on its 5 a 3’ -end sequence that does not match the 4G2 VL or VH mRNA
sequences (Mismatch) was included as an exemplary PCR primer that would not be consumed during
PCR, akin to multiplex repertoire primers. The reverse primer was biotinylated to facilitate detection
of captured PCR product on the bead.
4G2_VL_Fwd_Non-SA
TGGATCGTTACTAATATTCGC/iSp18fGGACTCAGACACTTCCGTGCGACATCAAGATGACC
CAGTCTC (SEQ ID NO: 10)
Mismatch_VL_Fwd_Non-SA
WO 19402
TGGATCGTTACTAATATTCGC/iSp 18/GGACTCAGACACTTCCGTGCATTGTGCTGACGCAA
ACTGTTA (SEQ ID NO: 20)
_Fwd_SA
TGGATCGTTACTAATATTCGC/iSp 18/GAATATTAGTAACGATCCAGGACTCGGACCGACAT
CAAGATGACCCAGTCTC (SEQ ID NO: 21)
Mismatch_VL_Fwd_SA
TGGATCGTTACTAATATTCGC/iSp 18/GAATATTAGTAACGATCCAGGACTCGGACCATTGT
GCTGACGCAAACTGTTA (SEQ ID NO: 22)
Mus_Kappa_ReV_Biotin
iotinTEG/ACCAGCAGAGCTCTCACCTGGTGCAGCATCAGCCC (SEQ ID NO: 13)
The underline designates the nnealing (complementary) sequences.
Component Volume (111) for 25 ul reaction
4G2 VL amplification primers (10 uM each) 0.5
4G2 RNA 0.1 (20 ng)
2X OneTaq One-Step RT-PCR Buffer (NEB) 12.5
OneTaq One-Step RT-PCR enzyme (NEB) 1
Oligo-conjugated beads 2
Mismatch_VL_Fwd oligo (varying concentrations, as listed below) 2.5
Water 4
Optiprep 2
RNase inhibitor 0.5
4G2 VL Amplification Fwd Primer Rev Primer
Primer Mix
# 1 4G2_VL_Fwd_Non-SA Mus_Kappa_ReV_Biotin
# 2 4G2_VL_Fwd_SA Mus_Kappa_ReV_Biotin
For competing mismatch oligo concentration used with the forward self-annealing primer
(4G2_VL_Fwd_SA), the following conditions were used:
Molar Ratio of Fwd Oligos Mismatch_VL_Fwd_SA
(Mismatch_VL_Fwd_SA : concentration (11M)
4G2_VL_Fwd_SA)
:1 30
:1 10
:1 5
1.25:1 2.5
0.625: 1 1.25
0:1 0
For competing mismatch oligo concentration used with the Fwd non-self—annealing primer
(4G2_VL_Fwd_Non-SA), the following ions were used:
Molar Ratio of Fwd Oligos
Mismatch_VL_Fwd_Non-
(Mismatch_VL_Fwd_Non-SA :
SA concentration (11M)
4G2_VL_Fwd_Non-SA )
:1 10
1:1 2
0.1:1 0.2
0.01:1 0.02
0:1 0
The samples were thermocycled with the following program:
After thermocycling, the samples were gently mixed by pipetting to d the beads. The
samples were then placed in a thermocycler with the following program to facilitate PCR product
capture onto beads:
Step Temp Time
1 70 0C 30 sec
2 55 OC 4 min
3 50 OC 4 min
4 45 °C 4 min
40 OC 3 min
7 4 OC hold
After the capturing ure, the tubes were applied to magnets and the atants were
recovered. The supernatants were analyzed by agarose gel electrophoresis to confirm successful
generation of PCR product. The beads, having captured PCR product, were washed three times with
100 pl ice-cold PBSA (1X PBS containing 1% BSA) using a magnet to sequester beads. Each sample
was then incubated with 100 pl of 1:500 AlexaFluor 647 IgG Fraction Monoclonal Mouse Anti-Biotin
(Jackson) in PBSA with rotation at 4°C and away from light for 45 minutes. After incubation, the
beads were washed three times with 100 pl ice-cold PBSA. After a final wash, beads were
resuspended with 100 pl ice-cold PBSA and analyzed with a flow cytometer for AlexaGluo647
fluorescence signal.
Mean fluorescence intensity (MFI) was determined for each sample. The sample data were
plotted as a percentage of MFI relative to MFI corresponding to absence of ch oligo. As
shown in FIGS. 7A-7B, only the self-annealing primers were capable of preventing PCR product
e competition at high ratios of ched primer oligo to matched primer oligo. These s
show that, specifically with the self-annealing primer design, the PCR product can be efficiently
ed under conditions of 15-fold more unused primer — conditions that, for example, simulate
multiple antibody repertoire conditions.
ORATION BY REFERENCE
All publications, patents, and Accession numbers ned herein are hereby incorporated
by reference in their entirety as if each individual publication or patent was specifically and
individually indicated to be incorporated by reference.
EQUIVALENTS
While specific embodiments of the subject invention have been discussed, the above
specification is illustrative and not restrictive. Many variations of the invention will become apparent
to those skilled in the art upon review of this specification and the claims below. The full scope of the
invention should be ined by reference to the claims, along with their full scope of equivalents,
and the specification, along with such variations.
Claims (47)
1. A method of making a nucleic acid sequence comprising a sequence that encodes a heavy chain element (HC element) of an antibody heavy chain variable region (HCVR) and a light chain element (LC t) of an antibody light chain variable region , and wherein the HCVR and LCVR are matched, the method comprising: a) acquiring an isolated production reaction site, comprising: i) a heavy chain (HC) strand, wherein the HC strand is a strand of a heavy chain double-stranded cDNA (HC ds cDNA) comprising a segment that encodes the HC element of 10 the HCVR from a cell; and ii) a light chain (LC) strand, wherein the LC strand is a strand of a light chain double- stranded cDNA (LC ds cDNA) comprising a segment that encodes the LC t of the LCVR from the cell, and b) covalent linking of the HC strand to the LC strand, 15 n the isolated production reaction site does not comprise a nucleic acid encoding an HCVR or an LCVR from a cell other than the cell, thereby making the nucleic acid sequence.
2. The method of claim 1, wherein the HC element comprises, or consists of, a heavy chain 20 variable region sequence (HCVRS), or an antigen binding nt thereof.
3. The method of claim 1 or 2, wherein the LC element comprises, or consists of, a light chain variable region sequence ), or an antigen binding fragment thereof. 25
4. The method of any of claims 1-3, wherein the nucleic acid sequence is configured such that, when expressed, the HC element and the LC element form a functional antigen binding molecule in vilro, ex vivo, or in vivo.
5. The method of any of claims 1-4, wherein acquiring the isolated production reaction site 30 ses: a) acquiring a capture substrate bound to: (i) a first -stranded cDNA (ds cDNA) comprising a strand that is complementary to a first mRNA that encodes the HCVR from the cell; and (ii) a second ds cDNA sing a strand complementary to a second mRNA 35 encoding the LCVR from the cell, to produce a loaded e substrate, and b) maintaining the isolated production reaction site under ions that allow amplification of the first and second ds cDNAs, to produce: a plurality of HC ds cDNAs comprising the segment that encodes the HC element of the HCVR from the cell; and a plurality of LC ds cDNAs comprising the segment that encodes an LC element of the LCVR from the cell.
6. The method of any of claims 1-5, wherein the e ate comprises a bead and a moiety which binds to cDNA.
7. The method of any of claims 1-6, wherein the ed production reaction site comprises a 10 reagent mixture suitable for producing, from the first and second mRNAs, a first ds cDNA comprising the segment that encodes the HC element of the HCVR of the cell, and a second ds cDNA comprising a t that encodes the LC element of the LCVR of the cell.
8. The method of any of claims 5-7, wherein the first and second ds cDNAs are ed in 15 the presence of primers, wherein at least one of the primers comprises a first member, a second member, and a nucleotide modification between the first and second members, wherein the nucleotide modification reduces DNA synthesis.
9. The method of claim 8, wherein the nucleotide modification comprises an insertion of a 20 spacer between two adjacent nucleotides or a modification to a ribose.
10. The method of claim 8 or 9, wherein the first member is capable of annealing with the second member in the same primer or a different , forming a double-stranded structure comprising a duplex region of 4, 5,6, 7, 8, 9, 10, ll, 12, 13, 14, 15, 16, 17, 18, 19, 20, more 25 basepairs.
11. The method of any of claims 8-10, n at least one of the primers is phosphorylated and comprises a sequence encoding at least a portion of a linker sequence, or a complementary sequence thereof.
12. The method of any of claims 1-1 1, n the HC ds cDNA comprises a 5’ overhang and a blunt end and the LC ds cDNA comprises a 5’ overhang and a blunt end.
13. The method of any of claims 1-12, wherein the HC strand and the LC strand are 35 covalently linked to produce a single stranded nucleic acid sequence, wherein the HC and LC strands are both sense strands or both antisense strands.
14. The method of any of claims 1-13, wherein the covalent linking occurs in an isolated linkage reaction site comprising a ligase.
15. The method of any of claims 1-14, wherein the HC strand and the LC strand are covalently linked in the presence of a splint oligonucleotide, wherein the splint oligonucleotide is hybridized to a ce comprising the junction of the HC strand and the LC strand to form a duplexed region at the site of linkage.
16. The method of claim 15, wherein the splint oligonucleotide comprises a modification that 10 inhibits DNA synthesis.
17. The method of any of claims l-l6, further sing, prior to acquiring the isolated production reaction site, ing an mRNA loaded capture substrate comprising: a) acquiring an isolated cell reaction site, comprising: 15 i) a cell; and ii) a capture ate capable of binding a first mRNA encoding an HCVR from the cell and a second mRNA ng an LCVR from the cell; and b) maintaining the ed cell reaction site under conditions that allow lysis of the cell and binding of the capture substrate with the first mRNA and the second mRNA to form the mRNA 20 loaded capture substrate, wherein the isolated cell reaction site does not include a nucleic acid encoding an HCVR or an LCVR from a cell other than the cell.
18. The method of claim 17, further sing releasing the mRNA loaded capture substrate 25 from the isolated cell reaction site in the presence of a poly(dA) or poly(dT) oligonucleotide.
19. The method of any of claims 1-18, further comprising amplifying the nucleic acid sequence. 30
20. The method of any of claims 1-19, further comprising cing all or a portion of the nucleic acid sequence.
21. The method of any of claims 1-20, r comprising inserting all or a portion of nucleic acid sequence into a vector.
22. The method of any of claims 1-21, comprising expressing the nucleic acid sequence to produce a polypeptide comprising the segment that encodes the HC element of the HCVR, and the segment that encodes the LC element of the LCVR.
23. The method of claim 22, further comprising contacting the ptide with an antigen and determining if the polypeptide binds the antigen.
24. A method of making a library comprising a plurality of unique members, the method comprising: 10 making the plurality of members by the method of any of claims 1-23, n each of the members comprises a sequence that encodes a heavy chain element (HC t) of a heavy chain variable region (HCVR) and a light chain element (LC element) of a light chain variable region (LCVR), wherein the HCVR and the LCVR are matched, and wherein each unique nucleic acid sequence of the ity comprises an HC element and an LC element from a 15 ent unique cell, thereby making the library.
25. The method of claim 24, wherein the library comprises one, two, three, or all of the following properties: 20 a) the plurality of unique s ses at least 104, 105, 106, 107, 108, or 109 unique members; b) the plurality of unique members comprises 104 to 109, 104 to 108, 104 to 107, 104 to 106, 104 to 105, 108 to 109, 107 to 109, 106 to 109, 105 to 109, 105 to 103, 106 to 107, 104 to 105, 105 to 106, 106 to 107, 107 to 108, or 108 to 109 unique members; 25 c) at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%, of the members in the library are unique members,; or d) less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1%, of the members in the library are unique members. 30
26. A library made by the method of claim 25.
27. The library of claim 26, wherein the library is a display library.
28. A method of making a binding polypeptide, the method comprising: 35 a) acquiring a library of claim 26 or 27; and b) sing a polypeptide encoded by a unique nucleic acid of the library.
29. The method of claim 28, further comprising contacting the polypeptide with an antigen and obtaining a nucleic acid that s a polypeptide that binds the n.
30. A method of making a nucleic acid sequence comprising a sequence that encodes an or chain element (AC element) of a T-cell receptor (TCR) or chain le region (ACVR) and a [5 chain element (BC element) of a TCR [3 chain variable region (BCVR), and wherein the ACVR and BCVR are matched, the method comprising: a) acquiring an isolated tion reaction site sing: i) an or chain (AC) , wherein the AC strand is a strand of an a chain double- 10 stranded cDNA (AC ds cDNA) comprising a segment that s an AC element of the ACVR from a cell; and ii) a [3 chain (BC) strand, wherein the BC strand is a strand of a [3 chain double- stranded cDNA (BC ds cDNA) comprising a segment that encodes a BC element of the BCVR from the cell, and 15 b) covalent linking of an AC strand to a BC strand, wherein the isolated production reaction site does not comprise a nucleic acid encoding a BCVR or an ACVR from a cell other than the cell, thereby making the nucleic acid sequence. 20
31. The method of claim 30, wherein the nucleic acid sequence is configured such that, when expressed, the AC element and the BC t form a functional antigen binding molecule.
32. The method of claim 31, wherein the covalent linking occurs in an isolated linkage on site comprising a ligase.
33. A method of making a library comprising a plurality of unique members, the method comprising: making the plurality of members by the method of any of claims 30-32, n each of the members comprises a sequence that encodes a or chain element (AC 30 element) of a or chain variable region (ACVR) and a [3 chain element (BC element) of a [3 chain variable region (BCVR), wherein the ACVR and BCVR are matched, and wherein each unique nucleic acid sequence of the plurality comprises an AC element and a BC element from a different unique cell, thereby making the library.
34. A library made by the method of claim 33.
35. A method of making a binding polypeptide, the method comprising: a) acquiring the library of claim 34; and b) expressing a polypeptide encoded by a unique nucleic acid of the library.
36. A method of making a nucleic acid sequence comprising a sequence that s an 7 chain element (GC element) of a TCR 7 chain variable region (GCVR) and a 5 chain element (DC element) of a TCR 5 chain variable region (DCVR), and wherein the GCVR and DCVR are d, the method comprising: a) acquiring an isolated production reaction site, sing: 10 i) a y chain (GC) , wherein the GC strand is a strand of a 7 chain double- stranded cDNA (GC ds cDNA) comprising a segment that encodes a GC element of the GCVR from a cell; and ii) a 5 chain (DC) strand, wherein the DC strand is a strand of a 5 chain double- ed cDNA (DC ds cDNA) comprising a segment that encodes a DC element of the 15 DCVR from the cell, and b) nt linking of a GC strand to a DC strand, wherein the isolated production reaction site does not comprises a nucleic acid encoding a DCVR or a GCVR from a cell other than the cell, thereby making the nucleic acid sequence.
37. The method of claim 36, wherein the nucleic acid sequence is ured such that, when expressed, the GC element and the DC element form a functional antigen binding le.
38. The method of claim 36 or 37, wherein the covalent linking occurs in an isolated linkage 25 reaction site comprising a ligase.
39. A method of making a library comprising a ity of unique s, the method comprising: making the plurality of members by the method of any of claims 36-38, 30 wherein each of the members ses a sequence that encodes a 7 chain element (GC element) of a 1/ chain variable region (GCVR) and a 5 chain element (DC element) of a 5 chain variable region (DCVR), wherein the GCVR and DCVR are matched, and wherein each unique nucleic acid sequence of the plurality comprises a GC element and a DC element from a different unique cell, 35 thereby making the library.
40. A library made by the method of claim 39.
41. A method of making a binding polypeptide, the method comprising: a) acquiring the library of claim 40; and b) expressing a polypeptide encoded by a unique nucleic acid of the library.
42. An isolated tion reaction site, comprising: a) a heavy chain (HC) strand, wherein the HC strand is a strand of a heavy chain double- ed cDNA (HC ds cDNA) comprising a segment that encodes an HC element of the HCVR from a cell; and 10 b) a light chain (LC) strand, wherein the LC strand is a strand of a light chain double-stranded cDNA (LC ds cDNA) comprising a segment that encodes an LC element of the LCVR from the cell, c) a primer comprising a first member, a second member, and a nucleotide modification between the first and second members, wherein the nucleotide modification s DNA synthesis, wherein the HCVR and LCVR are d, and wherein the isolated production reaction site 15 does not comprise a c acid encoding an LCVR or an HCVR from a cell other than the cell.
43. The isolated tion reaction site of claim 42, wherein the first member is capable of annealing with the second member in the same primer or a different , forming double-stranded structure comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, more basepairs. .
44. A self-annealing oligonucleotide comprising a first member, a second , and a spacer located between the first and second members, wherein the first member is capable of annealing with the second member in the same oligonucleotide or a different oligonucleotide, forming 25 a hairpin structure or a double-stranded structure comprising a duplex region of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19,20, more basepairs.
45. The oligonucleotide of claim 44, wherein the spacer is a flexible spacer or a PEG spacer. 30
46. An isolated linkage reaction site, comprising: a) a heavy chain (HC) strand, wherein the HC strand is a strand of a heavy chain double- stranded cDNA (HC ds cDNA) comprising a segment that s an HC element of the HCVR from a cell; b) a light chain (LC) strand, wherein the LC strand is a strand of a light chain -stranded 35 cDNA (LC ds cDNA) comprising a segment that encodes an LC element of the LCVR from the cell; c) a splint oligonucleotide that is capable of hybridizing to a sequence comprising the junction of the HC strand and the LC strand, to form a duplexed region at the site of linkage, wherein the HCVR and LCVR are matched, wherein the HC strand and the LC strand are covalently , and wherein the isolated linkage reaction site does not comprise a nucleic acid encoding an HCVR or an LCVR from a cell other than the cell,
47. The isolated linkage reaction site of claim 46, further comprising a ligase. Wage ammo, Wommc mfi Exam g Ex? mfi ”Exam E $0 E 30. E $0 3&5 E 85¢.me 3&5 SE35 ,8st .Nm mm 850.35 3% E Em 3 3% gm $333 £03 3cm Eon” mg E 0w («Emu nmfiommfi Eu 3 flung {2% cc 3 @338 £00m flag.9 so comma: Emu muSSQ Sufiwoa Emma: (2% 4&2me flu mu 3cm Wmfimmfi Embmgsm mv mam mm SE33 MI mnSQm u: 3333 wnfioa Wm Egommfi 3335 m: {Emu B E fiéu me + u: <2Qu Wmfimmfi 3 we «2% “E mumbfifi casmmfi: flu mam um E 95» mafia E <23.» hmfimmow .3 dzmu mo 39$me guise @355 QE< QE< m: mu. mu mm mm“ mm”. mm ,3 Emfi Na. mwgmzm 3 £338 83mg QE<.mm @335H Emfi c3 mvcgm 3 36% 8338 mm- 3:553 Emfi mm mvgmbm 3 338 $358 . .. WE .E 3 Eek 8 «ZmE Ema. Ow {ZmE Eat 3 E Fm E mmmmmm; gmbmgsm we wumflse mmammmh .mwmmeSm “5 mm «Egg cemmmofl .wme «Emu {Emu E EN Emam 33mm.“ .mwmsmomnm Emu cm 3mm <ZmE @338 munwei £03.39 SEE <2me .U cmu wkdwfimu $33th £55:QO ggfimmm mq. 33%th 838a .goWEWQm gowumwm 38% ““0Wmhfinwu $3 £338 am a Em <sz @3353 commmomm @333 .E gm Smbmga SUBSTITUTE SHEET (RULE 26) * 3 0339305 ccnjugated *1 03390 for mRNA capture {paw-{cm} .. HQ mRNA /* LC mRNA Beads; with ed mRNA in aqueaus phaga HQ 2% SUBSTITUTE SHEET (RULE 26) Spacer LCVR sequanae Compiementaryta Universaipriming bead ca ptur‘e oiiga sequence Lin ker Sequencas LCVR pmduct HC‘JR produqt SUBSTITUTE SHEET (RULE 26) SUBSTITUTE SHEET (RULE 26) €35 Fiexibfe LCVP‘ HCVR __Linker ........... Séq fer Seq far homeiagous hamoiogms remmbina’cion ination Fifi-3., 2E3 SUBSTITUTE SHEET (RULE 26) WO 19402 SUBSTITUTE SHEET (RULE 26) —amp§e-L 462 VH—VL m 800 m 600 9E1 0 Mini VH-VL —-— 460 462 VH—VL : W8 hp Mixed VH—VL product :2 5681581 bp 9E1 0 Mini VH—VL : 371 hp HG. 4B SUBSTITUTE SHEET (RULE 26) Primer PCR Produd VL sequence} {3} EZZF-- fi} (43 VH sticky-end PCR product Ffiifisfgik 16000%qnmmwmmmmnmmmmnm ‘" 8900€”_nwmmmmmnmnmwmmnn 4000?“ .....M "w M _____ EPCRFVGdUct IflflllfifllflfifilIflfllIflfilflfiflllflflfllflfllllflliflfilIflll SEEM-Gigs Biank VL-Qiigo VH-oiige ‘v’H-i-VL-aiégo ....................................................................................................................................................................................................................... SUBSTITUTE SHEET (RULE 26) 462 PCR NTC SUBSTITUTE SHEET (RULE 26) 10l10 PCR Praduct Capture 4mm Beads Uging Non‘Seifu Capture Anneaimg PCB 01290 123 ....................................................................... g ma~~~ Product g; saw g 68-- :1 45:2»- PCR a 28-~ 1% (3 VL 0:1 (101:1 Cam 1:1 5:”: Ratio of ah Oiiga : Matched {)Eigfl HQ. 72% PER Product Capture (into Beads Using Ssh“— Capture Anneaiing PCR Géigo 193W ...................... Pmduct PCR ‘Nm‘maiized 8Q .......... ............... £3 C3 49” .... 29m ............. VL {3 9:? 0952521 1.25:? 25:1 5:? ”35:1 Ratio 0? Mismamh Giiga : Matched Oiigo HQ. 7% SUBSTITUTE SHEET (RULE 26)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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US62/438,712 | 2016-12-23 |
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