AU2017298984B2 - Methods of purifying Fc-containing proteins - Google Patents

Methods of purifying Fc-containing proteins Download PDF

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Publication number
AU2017298984B2
AU2017298984B2 AU2017298984A AU2017298984A AU2017298984B2 AU 2017298984 B2 AU2017298984 B2 AU 2017298984B2 AU 2017298984 A AU2017298984 A AU 2017298984A AU 2017298984 A AU2017298984 A AU 2017298984A AU 2017298984 B2 AU2017298984 B2 AU 2017298984B2
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Australia
Prior art keywords
protein
temperature
elution buffer
region
amino acid
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AU2017298984A
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AU2017298984A1 (en
Inventor
John K. KAWOOYA
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Amgen Inc
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Amgen Inc
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Priority to AU2023222915A priority Critical patent/AU2023222915B2/en
Publication of AU2017298984B2 publication Critical patent/AU2017298984B2/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Storage Device Security (AREA)
AU2017298984A 2016-07-22 2017-07-21 Methods of purifying Fc-containing proteins Active AU2017298984B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2023222915A AU2023222915B2 (en) 2016-07-22 2023-08-31 Methods of purifying Fc-containing proteins

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662365943P 2016-07-22 2016-07-22
US62/365,943 2016-07-22
PCT/US2017/043384 WO2018018011A2 (en) 2016-07-22 2017-07-21 Methods of purifying fc-containing proteins

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2023222915A Division AU2023222915B2 (en) 2016-07-22 2023-08-31 Methods of purifying Fc-containing proteins

Publications (2)

Publication Number Publication Date
AU2017298984A1 AU2017298984A1 (en) 2019-02-07
AU2017298984B2 true AU2017298984B2 (en) 2023-08-31

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Family Applications (2)

Application Number Title Priority Date Filing Date
AU2017298984A Active AU2017298984B2 (en) 2016-07-22 2017-07-21 Methods of purifying Fc-containing proteins
AU2023222915A Active AU2023222915B2 (en) 2016-07-22 2023-08-31 Methods of purifying Fc-containing proteins

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2023222915A Active AU2023222915B2 (en) 2016-07-22 2023-08-31 Methods of purifying Fc-containing proteins

Country Status (12)

Country Link
US (1) US11312745B2 (https=)
EP (1) EP3487867B1 (https=)
JP (2) JP7092744B2 (https=)
KR (2) KR102579850B1 (https=)
CN (2) CN109563125B (https=)
AU (2) AU2017298984B2 (https=)
CA (2) CA3218290A1 (https=)
IL (2) IL285146B (https=)
MA (1) MA45721A (https=)
MX (2) MX2019000903A (https=)
SG (1) SG11201900573UA (https=)
WO (1) WO2018018011A2 (https=)

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CN112839959A (zh) * 2018-09-21 2021-05-25 特尼奥生物股份有限公司 用于纯化异源二聚多特异性抗体的方法
AU2019356564A1 (en) * 2018-10-11 2021-04-29 Amgen Inc. Downstream processing of bispecific antibody constructs
CA3123052A1 (en) * 2019-01-16 2020-07-23 Regeneron Pharmaceuticals, Inc. Method and system of identifying and quantifying antibody fragmentation
KR102286892B1 (ko) * 2019-07-08 2021-08-06 삼천당제약주식회사 안과용 단백질 제제의 정제방법
CN110724204B (zh) * 2019-11-18 2021-10-22 广东菲鹏制药股份有限公司 Fc融合蛋白的纯化方法
CN112881675A (zh) * 2019-11-29 2021-06-01 广东菲鹏生物有限公司 IgM抗体检测稀释液
BR112022019841A2 (pt) 2020-04-09 2022-12-06 Cytomx Therapeutics Inc Composições contendo anticorpos ativáveis
EP4213958B1 (en) * 2020-09-16 2026-02-18 Waters Technologies Corporation Improved size exclusion chromatography utilizing low concentration amino acids in size exclusion chromatography mobile phase
CN118414350A (zh) * 2021-10-19 2024-07-30 阿特根公司 纯化具有igg fc结构域的融合蛋白的方法
CN113980092B (zh) * 2021-12-09 2024-05-14 上海药明生物技术有限公司 一种蛋白质亲和纯化方法
CN116165290B (zh) * 2021-12-17 2025-06-27 复旦大学 一种抗体药物研发流程中工程细胞株的筛选方法
CN116271982B (zh) * 2022-11-30 2024-01-09 杭州广科安德生物科技有限公司 一种血液高丰度蛋白免疫亲和柱的再生方法

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Also Published As

Publication number Publication date
AU2023222915B2 (en) 2026-01-22
KR102579850B1 (ko) 2023-09-18
CN115925780A (zh) 2023-04-07
CA3218290A1 (en) 2018-01-25
IL264375A (en) 2019-02-28
KR102435801B1 (ko) 2022-08-25
CN109563125A (zh) 2019-04-02
MX2023006291A (es) 2023-06-13
SG11201900573UA (en) 2019-02-27
CA3031469C (en) 2025-11-18
WO2018018011A3 (en) 2018-03-01
MA45721A (fr) 2019-05-29
JP7411020B2 (ja) 2024-01-10
CA3031469A1 (en) 2018-01-25
IL285146A (en) 2021-08-31
EP3487867A2 (en) 2019-05-29
KR20190037247A (ko) 2019-04-05
US11312745B2 (en) 2022-04-26
AU2023222915A1 (en) 2023-09-21
EP3487867B1 (en) 2026-04-01
IL264375B (en) 2021-09-30
US20190127418A1 (en) 2019-05-02
WO2018018011A2 (en) 2018-01-25
MX2019000903A (es) 2019-05-15
JP2022120138A (ja) 2022-08-17
CN109563125B (zh) 2022-08-09
AU2017298984A1 (en) 2019-02-07
CN115925780B (zh) 2025-11-04
JP2019528255A (ja) 2019-10-10
IL285146B (en) 2022-09-01
KR20220120716A (ko) 2022-08-30
JP7092744B2 (ja) 2022-06-28

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