MA45721A - Procédés de purification de protéines contenant des fragments fc - Google Patents

Procédés de purification de protéines contenant des fragments fc

Info

Publication number
MA45721A
MA45721A MA045721A MA45721A MA45721A MA 45721 A MA45721 A MA 45721A MA 045721 A MA045721 A MA 045721A MA 45721 A MA45721 A MA 45721A MA 45721 A MA45721 A MA 45721A
Authority
MA
Morocco
Prior art keywords
fragments
processes
protein containing
purifying protein
purifying
Prior art date
Application number
MA045721A
Other languages
English (en)
Inventor
John K Kawooya
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of MA45721A publication Critical patent/MA45721A/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Storage Device Security (AREA)
MA045721A 2016-07-22 2017-07-21 Procédés de purification de protéines contenant des fragments fc MA45721A (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201662365943P 2016-07-22 2016-07-22

Publications (1)

Publication Number Publication Date
MA45721A true MA45721A (fr) 2019-05-29

Family

ID=59656168

Family Applications (1)

Application Number Title Priority Date Filing Date
MA045721A MA45721A (fr) 2016-07-22 2017-07-21 Procédés de purification de protéines contenant des fragments fc

Country Status (12)

Country Link
US (1) US11312745B2 (fr)
EP (1) EP3487867A2 (fr)
JP (2) JP7092744B2 (fr)
KR (2) KR102435801B1 (fr)
CN (2) CN109563125B (fr)
AU (2) AU2017298984B2 (fr)
CA (2) CA3031469A1 (fr)
IL (2) IL285146B (fr)
MA (1) MA45721A (fr)
MX (2) MX2019000903A (fr)
SG (1) SG11201900573UA (fr)
WO (1) WO2018018011A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210355215A1 (en) * 2018-09-21 2021-11-18 Teneobio, Inc. Methods for purifying heterodimeric, multispecific antibodies
US20210395298A1 (en) * 2018-10-11 2021-12-23 Amgen Inc. Downstream processing of bispecific antibody constructs
KR102286892B1 (ko) * 2019-07-08 2021-08-06 삼천당제약주식회사 안과용 단백질 제제의 정제방법
CN110724204B (zh) * 2019-11-18 2021-10-22 广东菲鹏制药股份有限公司 Fc融合蛋白的纯化方法
CN112881675A (zh) * 2019-11-29 2021-06-01 广东菲鹏生物有限公司 IgM抗体检测稀释液
CN116056775A (zh) * 2020-09-16 2023-05-02 沃特世科技公司 在尺寸排阻色谱流动相中使用低浓度氨基酸的改善尺寸排阻色谱法
KR20230057280A (ko) * 2021-10-19 2023-04-28 (주)알테오젠 IgG Fc 도메인을 가지는 융합 단백질의 정제방법
CN116271982B (zh) * 2022-11-30 2024-01-09 杭州广科安德生物科技有限公司 一种血液高丰度蛋白免疫亲和柱的再生方法

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4968607A (en) 1987-11-25 1990-11-06 Immunex Corporation Interleukin-1 receptors
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
WO1990005183A1 (fr) 1988-10-31 1990-05-17 Immunex Corporation Recepteurs d'interleukine-4
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6713610B1 (en) 1990-01-12 2004-03-30 Raju Kucherlapati Human antibodies derived from immunized xenomice
WO1991010741A1 (fr) 1990-01-12 1991-07-25 Cell Genesys, Inc. Generation d'anticorps xenogeniques
US6673986B1 (en) 1990-01-12 2004-01-06 Abgenix, Inc. Generation of xenogeneic antibodies
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
WO1991018982A1 (fr) 1990-06-05 1991-12-12 Immunex Corporation Recepteurs de l'interleukine-1 de type ii
US5350683A (en) 1990-06-05 1994-09-27 Immunex Corporation DNA encoding type II interleukin-1 receptors
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US5874299A (en) 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6300129B1 (en) 1990-08-29 2001-10-09 Genpharm International Transgenic non-human animals for producing heterologous antibodies
ES2108048T3 (es) 1990-08-29 1997-12-16 Genpharm Int Produccion y utilizacion de animales inferiores transgenicos capaces de producir anticuerpos heterologos.
US5877397A (en) 1990-08-29 1999-03-02 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US6255458B1 (en) 1990-08-29 2001-07-03 Genpharm International High affinity human antibodies and human antibodies against digoxin
CA2140638C (fr) 1992-07-24 2010-05-04 Raju Kucherlapati Production d'anticorps xenogeniques
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US6130364A (en) 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
CA2219361C (fr) 1995-04-27 2012-02-28 Abgenix, Inc. Anticorps humains derives d'une xenosouris immunisee
EP1500329B1 (fr) 1996-12-03 2012-03-21 Amgen Fremont Inc. Les anticorps humains qui lient en particulier l'alpha de TNF humain
US6271349B1 (en) 1996-12-23 2001-08-07 Immunex Corporation Receptor activator of NF-κB
WO1999053059A1 (fr) * 1998-04-16 1999-10-21 Genentech, Inc. Secretion de proteines glycosylees au moyen d'une pro-sequence de l'activateur tissulaire du plasminogene
US6660843B1 (en) 1998-10-23 2003-12-09 Amgen Inc. Modified peptides as therapeutic agents
US20030133939A1 (en) 2001-01-17 2003-07-17 Genecraft, Inc. Binding domain-immunoglobulin fusion proteins
JP5553963B2 (ja) 2004-10-22 2014-07-23 アムジエン・インコーポレーテツド 組換え抗体をリフォールディングする方法
CA2636109A1 (fr) * 2006-01-06 2007-07-19 Amgen Inc. Procedes et systemes d'isolation de molecules cibles de solutions complexes par chromatographie sur colonne a l'aide d'eluants contenant des solvants organiques
MX363905B (es) 2006-06-12 2019-04-08 Aptevo Res & Development Llc Proteinas de union multivalentes monocatenarias con funcion efectora.
US10982250B2 (en) * 2006-09-18 2021-04-20 Genentech, Inc. Methods of protein production
US8198409B2 (en) * 2007-05-21 2012-06-12 Nomadic Bioscience Co., Ltd. Polypeptide, an affinity chromatography material, and a method for separating and/or purifying immunoglobulin
WO2009089004A1 (fr) 2008-01-07 2009-07-16 Amgen Inc. Méthode de fabrication de molécules hétérodimères fc d'anticorps utilisant les effets de conduite électrostatique
SG2013061528A (en) * 2008-08-14 2015-03-30 Merck Sharp & Dohme Methods for purifying antibodies using protein a affinity chromatography
CN102482321B (zh) 2009-06-22 2015-06-17 安姆根有限公司 使用化学控制的氧化还原态重折叠蛋白质
AU2010266093B2 (en) * 2009-06-25 2013-06-27 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
CN104945509A (zh) 2009-09-16 2015-09-30 弗·哈夫曼-拉罗切有限公司 包含卷曲螺旋和/或系链的蛋白质复合体及其用途
CN102382190B (zh) 2010-09-01 2014-04-09 山东新时代药业有限公司 分离和去除TNFR-Fc融合蛋白中寡聚体的方法
EP2657254A4 (fr) 2010-12-24 2014-01-08 Asahi Kasei Medical Co Ltd Procédé d'immobilisation d'une protéine a thermosensible
DK2729482T3 (en) 2011-07-08 2018-05-07 Merck Sharp & Dohme PROCEDURE FOR CLEANING FC-FUSION PROTEIN
WO2013147691A1 (fr) 2012-03-28 2013-10-03 Ge Healthcare Bio-Sciences Ab Matrice de chromatographie par affinité
EP2889617B1 (fr) 2012-08-27 2017-10-11 Asahi Kasei Medical Co., Ltd. Procédé de purification d'anticorps au moyen d'une chromatographie sensible à la température
UY35148A (es) * 2012-11-21 2014-05-30 Amgen Inc Immunoglobulinas heterodiméricas
US20140302037A1 (en) 2013-03-15 2014-10-09 Amgen Inc. BISPECIFIC-Fc MOLECULES
EP3176185A1 (fr) * 2013-11-04 2017-06-07 Glenmark Pharmaceuticals S.A. Production lymphocytes t de reciblage d'immunoglobulines hétéro-dimères

Also Published As

Publication number Publication date
IL264375A (en) 2019-02-28
CA3031469A1 (fr) 2018-01-25
MX2023006291A (es) 2023-06-13
WO2018018011A3 (fr) 2018-03-01
KR20190037247A (ko) 2019-04-05
US20190127418A1 (en) 2019-05-02
CN109563125B (zh) 2022-08-09
AU2023222915A1 (en) 2023-09-21
WO2018018011A2 (fr) 2018-01-25
CN109563125A (zh) 2019-04-02
JP7092744B2 (ja) 2022-06-28
KR20220120716A (ko) 2022-08-30
AU2017298984A1 (en) 2019-02-07
JP7411020B2 (ja) 2024-01-10
IL285146B (en) 2022-09-01
IL264375B (en) 2021-09-30
MX2019000903A (es) 2019-05-15
KR102435801B1 (ko) 2022-08-25
JP2019528255A (ja) 2019-10-10
IL285146A (en) 2021-08-31
KR102579850B1 (ko) 2023-09-18
CN115925780A (zh) 2023-04-07
CA3218290A1 (fr) 2018-01-25
US11312745B2 (en) 2022-04-26
AU2017298984B2 (en) 2023-08-31
SG11201900573UA (en) 2019-02-27
EP3487867A2 (fr) 2019-05-29
JP2022120138A (ja) 2022-08-17

Similar Documents

Publication Publication Date Title
MA45721A (fr) Procédés de purification de protéines contenant des fragments fc
MA39774A (fr) Procédés chimiques pour produire des nucléotides étiquetés
EP3408219A4 (fr) Appareil de séquençage d'adn massivement parallèle
MA41294A (fr) Anticorps anti-myostatine, polypeptides contenant des variants de régions fc, et procédés d'utilisation
FR3014901B1 (fr) Procede de purification de virus ou vecteurs viraux enveloppes
EP3427824A4 (fr) Catalyseur pour purification de gaz d'échappement
FR3025515B1 (fr) Procede de purification d'un anticorps monoclonal
MA41941A (fr) Procédé de purification de protéines
IL261040B (en) Protein purification
SG11201610431WA (en) Methods and reagents for purification of proteins
EP3275544A4 (fr) Catalyseur de purification des gaz d'échappement
GB201705213D0 (en) Exhaust gas purification filter
PL3513821T3 (pl) Materiał do oczyszczania krwi
HUE055160T2 (hu) Eljárás klorid-tartalmú reakcióoldatok elektrokémiai tisztítására
EP3438426A4 (fr) Système de purification d'échappement
FR2990878B1 (fr) Procede de purification de melanges gazeux contenant des gaz acides.
EP3318533A4 (fr) Cuivre supporté sur zéolite et catalyseur de traitement de purification de gaz d'échappement contenant ladite zéolite
MA41297A (fr) Système de purification de liquides
EP3341112A4 (fr) Procédés et compositions de purification de protéine et de mise en réaction d'une enzyme
MA41961A (fr) Procédés de régénération d'adsorbants solides
HK1257713A1 (zh) 用於蛋白純化的方法
DE112016002454A5 (de) Biologisch-chemisch reaktives Verfahren zur Abluftreinigung
FR3027821B1 (fr) Procede de purification d'oses.
IL258745A (en) Improved purification of tgf-beta superfamily proteins
EP3365365A4 (fr) Procédés de purification de protéines du plasma