AU2013284425B2 - Techniques and droplet actuator designs for reducing bubble formation - Google Patents

Techniques and droplet actuator designs for reducing bubble formation Download PDF

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Publication number
AU2013284425B2
AU2013284425B2 AU2013284425A AU2013284425A AU2013284425B2 AU 2013284425 B2 AU2013284425 B2 AU 2013284425B2 AU 2013284425 A AU2013284425 A AU 2013284425A AU 2013284425 A AU2013284425 A AU 2013284425A AU 2013284425 B2 AU2013284425 B2 AU 2013284425B2
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Prior art keywords
droplet
droplet operations
gap
operations
actuator
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AU2013284425A1 (en
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Cyril Delattre
Arnaud Rival
Vijay Srinivasan
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Illumina France Sarl
Advanced Liquid Logic Inc
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Illumina France Sarl
Advanced Liquid Logic Inc
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Assigned to ADVANCED LIQUID LOGIC INC., ILLUMINA FRANCE reassignment ADVANCED LIQUID LOGIC INC. Amend patent request/document other than specification (104) Assignors: ADVANCED LIQUID LOGIC INC.
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
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    • B01L2300/08—Geometry, shape and general structure
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/04—Moving fluids with specific forces or mechanical means
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    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/04—Moving fluids with specific forces or mechanical means
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    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
    • B01L2400/0448—Marangoni flow; Thermocapillary effect
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Coating Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Particle Formation And Scattering Control In Inkjet Printers (AREA)
  • Reciprocating Pumps (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
AU2013284425A 2012-06-27 2013-06-27 Techniques and droplet actuator designs for reducing bubble formation Active AU2013284425B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261664980P 2012-06-27 2012-06-27
US61/664,980 2012-06-27
US201261666417P 2012-06-29 2012-06-29
US61/666,417 2012-06-29
US201261678263P 2012-08-01 2012-08-01
US61/678,263 2012-08-01
PCT/US2013/048319 WO2014004908A1 (en) 2012-06-27 2013-06-27 Techniques and droplet actuator designs for reducing bubble formation

Publications (2)

Publication Number Publication Date
AU2013284425A1 AU2013284425A1 (en) 2015-01-29
AU2013284425B2 true AU2013284425B2 (en) 2017-07-27

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US (2) US9815061B2 (enExample)
EP (1) EP2867645B1 (enExample)
JP (1) JP6222671B2 (enExample)
KR (1) KR102070330B1 (enExample)
CN (1) CN104603595B (enExample)
AU (1) AU2013284425B2 (enExample)
BR (1) BR112014032727B1 (enExample)
CA (1) CA2877950C (enExample)
IL (1) IL236459A0 (enExample)
IN (1) IN2015DN00359A (enExample)
WO (1) WO2014004908A1 (enExample)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140216559A1 (en) * 2013-02-07 2014-08-07 Advanced Liquid Logic, Inc. Droplet actuator with local variation in gap height to assist in droplet splitting and merging operations
WO2014135864A1 (en) * 2013-03-05 2014-09-12 Micromass Uk Limited Charging plate for enhancing multiply charged ions by laser desorption
WO2014201341A1 (en) * 2013-06-14 2014-12-18 Advanced Liquid Logic, Inc. Droplet actuator and methods
EP3131758B1 (en) * 2014-04-16 2021-11-24 Abbott Laboratories Droplet actuator fabrication apparatus, systems, and related methods
CA2944994C (en) 2014-04-24 2021-01-19 Diassess Inc. Colorimetric detection of nucleic acid amplification
WO2015196303A1 (en) * 2014-06-27 2015-12-30 Uvic Industry Partnerships Inc. System and method for matrix-coating samples for mass spectrometry
CN107249743B (zh) * 2014-12-31 2020-01-10 雅培制药有限公司 数字微流控稀释设备、系统及相关方法
WO2016151029A1 (en) * 2015-03-23 2016-09-29 Stamford Devices Limited An aerosol generator
US10016759B2 (en) * 2015-04-10 2018-07-10 University Of Macau Cooperative-electrode driving technique for droplet-velocity improvement of digital microfluidic systems
CN108026494A (zh) 2015-06-05 2018-05-11 米罗库鲁斯公司 限制蒸发和表面结垢的空气基质数字微流控装置和方法
CN208562324U (zh) 2015-06-05 2019-03-01 米罗库鲁斯公司 空气基质数字微流控(dmf)装置
US10906044B2 (en) 2015-09-02 2021-02-02 Illumina Cambridge Limited Methods of improving droplet operations in fluidic systems with a filler fluid including a surface regenerative silane
CN109153016B (zh) * 2015-09-02 2021-12-28 帝肯贸易股份公司 微流体中的磁导管
EP3365108B1 (en) * 2015-10-22 2024-06-12 Illumina, Inc. Filler fluid for fluidic devices
EP3429543B1 (en) 2016-03-14 2024-11-20 Pfizer Inc. Selectively vented biological assay devices and associated methods
WO2017160836A1 (en) 2016-03-14 2017-09-21 Diassess Inc. Systems and methods for performing biological assays
PT3430378T (pt) 2016-03-14 2022-09-15 Lucira Health Inc Dispositivos e métodos para modificar propriedades ópticas
KR101891401B1 (ko) * 2016-08-12 2018-08-23 고려대학교 산학협력단 미세유체 소자 및 이의 제조방법
WO2018039281A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
EP3563151A4 (en) 2016-12-28 2020-08-19 Miroculus Inc. DIGITAL MICROFLUIDIC DEVICES AND METHODS
US10596567B2 (en) * 2017-03-27 2020-03-24 International Business Machines Corporation Microfluidic ratchets for displacing particles
WO2018187476A1 (en) 2017-04-04 2018-10-11 Miroculus Inc. Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets
US11080848B2 (en) 2017-04-06 2021-08-03 Lucira Health, Inc. Image-based disease diagnostics using a mobile device
EP3582894A4 (en) 2017-04-21 2020-03-04 Hewlett-Packard Development Company, L.P. MICROFLUIDIC CHIP
US11235328B2 (en) * 2017-04-21 2022-02-01 Hewlett-Packard Development Company, L.P. Coplanar microfluidic manipulation
US11364496B2 (en) 2017-04-21 2022-06-21 Hewlett-Packard Development Company, L.P. Coplanar fluidic interconnect
US11278892B2 (en) 2017-04-21 2022-03-22 Hewlett-Packard Development Company, L.P. Chip to chip fluidic interconnect
EP3641934B1 (en) * 2017-06-21 2024-09-25 Lightcast Discovery Ltd Microdroplet manipulation device
WO2019023133A1 (en) 2017-07-24 2019-01-31 Miroculus Inc. DIGITAL MICROFLUIDIC SYSTEMS AND METHODS WITH INTEGRATED PLASMA COLLECTION DEVICE
US10369570B2 (en) 2017-07-27 2019-08-06 Sharp Life Science (Eu) Limited Microfluidic device with droplet pre-charge on input
WO2019046860A1 (en) 2017-09-01 2019-03-07 Miroculus Inc. DIGITAL MICROFLUIDIC DEVICES AND METHODS OF USE
US10549275B2 (en) 2017-09-14 2020-02-04 Lucira Health, Inc. Multiplexed biological assay device with electronic readout
WO2019055135A1 (en) 2017-09-14 2019-03-21 Diassess Inc. MULTIPLEXED BIOLOGICAL ASSAY DEVICE WITH ELECTRONIC READING
WO2019099306A1 (en) * 2017-11-14 2019-05-23 Illumina, Inc. Droplet dispensing
US11779926B2 (en) * 2017-11-14 2023-10-10 Illumina, Inc. Digital fluidic cartridge with inlet gap height larger than outlet gap height
WO2019177589A1 (en) * 2018-03-13 2019-09-19 Hewlett-Packard Development Company, L.P. Microfluidic devices
CN112469504B (zh) 2018-05-23 2024-08-16 米罗库鲁斯公司 对数字微流控中的蒸发的控制
JP7022844B2 (ja) * 2018-10-17 2022-02-18 株式会社日立ハイテク 生化学用カートリッジ及び生化学分析装置
CN112585474B (zh) * 2018-10-19 2024-06-21 株式会社日立高新技术 生物化学用盒和生物化学分析装置
CN109375365A (zh) * 2018-11-30 2019-02-22 重庆秉为科技有限公司 一种电湿润液滴形状可调的显示装置
USD907232S1 (en) 2018-12-21 2021-01-05 Lucira Health, Inc. Medical testing device
CN113543883A (zh) 2019-01-31 2021-10-22 米罗库鲁斯公司 非结垢组合物以及用于操控和处理包封的微滴的方法
AU2020232685B2 (en) * 2019-03-05 2024-12-19 Pfizer Inc. Bubble-free liquid filling of fluidic chambers
EP3953041A4 (en) 2019-04-08 2023-01-25 Miroculus Inc. MULTI-CARTRIDGE DIGITAL MICROFLUID DEVICES AND METHODS OF USE
WO2021016614A1 (en) 2019-07-25 2021-01-28 Miroculus Inc. Digital microfluidics devices and methods of use thereof
US11458467B2 (en) * 2019-08-06 2022-10-04 Bio-Rad Laboratories Inc. Structures to define flow confinement shape and confinement stability with uniform aspiration
CN114829626A (zh) 2019-10-10 2022-07-29 1859公司 用于微流体筛选的方法和系统
US11946901B2 (en) 2020-01-27 2024-04-02 Nuclera Ltd Method for degassing liquid droplets by electrical actuation at higher temperatures
USD953561S1 (en) 2020-05-05 2022-05-31 Lucira Health, Inc. Diagnostic device with LED display
CN111569961B (zh) * 2020-05-18 2021-11-30 华南师范大学 一种一次性纸基数字微流控检测芯片及其检测方法
USD962470S1 (en) 2020-06-03 2022-08-30 Lucira Health, Inc. Assay device with LCD display
US11777422B2 (en) * 2021-01-06 2023-10-03 Massachusetts Institute Of Technology Multilayered microhydraulic actuators
JP7528850B2 (ja) * 2021-04-12 2024-08-06 トヨタ自動車株式会社 アクチュエータ
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation
CN116510791A (zh) * 2022-01-24 2023-08-01 广东奥素液芯微纳科技有限公司 基于数字微流控芯片的液滴分离装置及分离方法
KR102868980B1 (ko) * 2022-12-15 2025-10-02 숭실대학교산학협력단 3차원 액적 제어 장치 및 방법

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6911132B2 (en) * 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
WO2008038810A1 (en) * 2006-09-25 2008-04-03 Sumitomo Chemical Company, Limited Process for the preparation of 2-hydr0xy-4- (alkylthio or arylthio) butanoic acid by microbial conversion of 2-0x0-4- (alkylthio or arylthio) 1-butanol employing bacteria of the genus pseud0m0nas, rh0d0c0ccus, or bacillus
US20090280475A1 (en) * 2006-04-18 2009-11-12 Pollack Michael G Droplet-based pyrosequencing
US20090311713A1 (en) * 2008-05-13 2009-12-17 Advanced Liquid Logic, Inc. Method of Detecting an Analyte
WO2012012090A2 (en) * 2010-06-30 2012-01-26 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
WO2012037308A2 (en) * 2010-09-16 2012-03-22 Advanced Liquid Logic, Inc. Droplet actuator systems, devices and methods

Family Cites Families (206)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4127460A (en) 1976-10-27 1978-11-28 Desoto, Inc. Radiation-curing aqueous coatings providing a nonadherent surface
US4244693A (en) 1977-02-28 1981-01-13 The United States Of America As Represented By The United States Department Of Energy Method and composition for testing for the presence of an alkali metal
FR2543320B1 (fr) 1983-03-23 1986-01-31 Thomson Csf Dispositif indicateur a commande electrique de deplacement d'un fluide
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US6013531A (en) 1987-10-26 2000-01-11 Dade International Inc. Method to use fluorescent magnetic polymer particles as markers in an immunoassay
US5225332A (en) 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
GB8917963D0 (en) 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
US5266498A (en) 1989-10-27 1993-11-30 Abbott Laboratories Ligand binding assay for an analyte using surface-enhanced scattering (SERS) signal
US5181016A (en) 1991-01-15 1993-01-19 The United States Of America As Represented By The United States Department Of Energy Micro-valve pump light valve display
US5498392A (en) 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
WO1994008759A1 (en) 1992-10-16 1994-04-28 Thomas Jefferson University Method and apparatus for robotically performing sanger dideoxynucleotide dna sequencing reactions
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
US6152181A (en) 1992-11-16 2000-11-28 The United States Of America As Represented By The Secretary Of The Air Force Microdevices based on surface tension and wettability that function as sensors, actuators, and other devices
ATE208658T1 (de) 1993-07-28 2001-11-15 Pe Corp Ny Vorrichtung und verfahren zur nukleinsäurevervielfältigung
US5486337A (en) 1994-02-18 1996-01-23 General Atomics Device for electrostatic manipulation of droplets
US6673533B1 (en) 1995-03-10 2004-01-06 Meso Scale Technologies, Llc. Multi-array multi-specific electrochemiluminescence testing
US6319668B1 (en) 1995-04-25 2001-11-20 Discovery Partners International Method for tagging and screening molecules
CA2176053C (en) 1995-05-09 1999-10-05 Yoshihiro Kinoshita Method and apparatus for agglutination immunoassay
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US5945281A (en) 1996-02-02 1999-08-31 Becton, Dickinson And Company Method and apparatus for determining an analyte from a sample fluid
DE19717085C2 (de) 1997-04-23 1999-06-17 Bruker Daltonik Gmbh Verfahren und Geräte für extrem schnelle DNA-Vervielfachung durch Polymerase-Kettenreaktionen (PCR)
US5998224A (en) 1997-05-16 1999-12-07 Abbott Laboratories Magnetically assisted binding assays utilizing a magnetically responsive reagent
US20020001544A1 (en) 1997-08-28 2002-01-03 Robert Hess System and method for high throughput processing of droplets
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
DE19822123C2 (de) 1997-11-21 2003-02-06 Meinhard Knoll Verfahren und Vorrichtung zum Nachweis von Analyten
US6063339A (en) 1998-01-09 2000-05-16 Cartesian Technologies, Inc. Method and apparatus for high-speed dot array dispensing
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6632655B1 (en) 1999-02-23 2003-10-14 Caliper Technologies Corp. Manipulation of microparticles in microfluidic systems
EP1041386B1 (en) 1999-03-25 2007-10-17 Tosoh Corporation Analyzer
IT1309430B1 (it) 1999-05-18 2002-01-23 Guerrieri Roberto Metodo ed apparato per la manipolazione di particelle per mezzo delladielettroforesi
FR2794039B1 (fr) 1999-05-27 2002-05-03 Osmooze Sa Dispositif de formation, de deplacement et de diffusion de petites quantites calibrees de liquides
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US20030027204A1 (en) 1999-09-03 2003-02-06 Yokogawa Electric Corporation, A Japan Corporation Method and apparatus for producing biochips
US20040209376A1 (en) 1999-10-01 2004-10-21 Surromed, Inc. Assemblies of differentiable segmented particles
ATE328670T1 (de) 1999-11-11 2006-06-15 Trinity College Dublin Vorrichtung und verfahren zur verabreichung von tropfen
US6924792B1 (en) 2000-03-10 2005-08-02 Richard V. Jessop Electrowetting and electrostatic screen display systems, colour displays and transmission means
JP3442338B2 (ja) 2000-03-17 2003-09-02 株式会社日立製作所 Ｄｎａ分析装置、ｄｎａ塩基配列決定装置、ｄｎａ塩基配列決定方法、および反応モジュール
WO2002007503A1 (en) 2000-07-25 2002-01-31 The Regents Of The University Of California Electrowetting-driven micropumping
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
AU2001290879A1 (en) 2000-09-15 2002-03-26 California Institute Of Technology Microfabricated crossflow devices and methods
US6453928B1 (en) 2001-01-08 2002-09-24 Nanolab Ltd. Apparatus, and method for propelling fluids
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US7211442B2 (en) 2001-06-20 2007-05-01 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US6734436B2 (en) 2001-08-07 2004-05-11 Sri International Optical microfluidic devices and methods
US6995024B2 (en) 2001-08-27 2006-02-07 Sri International Method and apparatus for electrostatic dispensing of microdroplets
US20040231987A1 (en) 2001-11-26 2004-11-25 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
US7163612B2 (en) * 2001-11-26 2007-01-16 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
US7147763B2 (en) 2002-04-01 2006-12-12 Palo Alto Research Center Incorporated Apparatus and method for using electrostatic force to cause fluid movement
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
FR2842747B1 (fr) 2002-07-23 2004-10-15 Commissariat Energie Atomique Procede et dispositif pour le criblage de molecules dans des cellules
FR2843048B1 (fr) 2002-08-01 2004-09-24 Commissariat Energie Atomique Dispositif d'injection et de melange de micro-gouttes liquides.
US6989234B2 (en) 2002-09-24 2006-01-24 Duke University Method and apparatus for non-contact electrostatic actuation of droplets
US7329545B2 (en) 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US20040055871A1 (en) 2002-09-25 2004-03-25 The Regents Of The University Of California Use of ion beams for protecting substrates from particulate defect contamination in ultra-low-defect coating processes
US7217542B2 (en) 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
US7845749B2 (en) * 2002-11-13 2010-12-07 Sony Corporation Liquid-ejecting method and liquid-ejecting apparatus
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
GB0304033D0 (en) 2003-02-21 2003-03-26 Imp College Innovations Ltd Apparatus
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
JP4404672B2 (ja) 2003-05-28 2010-01-27 セイコーエプソン株式会社 液滴吐出ヘッド、液滴吐出ヘッドの製造方法、マイクロアレイ製造装置、及びマイクロアレイの製造方法
CN1267565C (zh) * 2003-06-09 2006-08-02 清华大学 微液滴振荡型硅槽式聚合酶链式反应生物芯片
TWI230760B (en) * 2003-07-29 2005-04-11 Univ Tsinghua Electrowetting electrode design with electromagnetic field for actuation of the magnetic-beads biochemical detection system
US7767435B2 (en) 2003-08-25 2010-08-03 University Of Washington Method and device for biochemical detection and analysis of subcellular compartments from a single cell
WO2005040228A2 (en) 2003-10-24 2005-05-06 Adhesives Research, Inc. Disintegratable films for diagnostic devices
JP2005139011A (ja) 2003-11-04 2005-06-02 Nof Corp 火薬原料及びその製造方法
JP4773360B2 (ja) 2003-11-17 2011-09-14 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 流体を操作するためのシステム
JP2007518991A (ja) 2004-01-14 2007-07-12 ルミネックス・コーポレーション ダイナミックレンジを拡大する方法及びシステム
WO2005069015A1 (ja) 2004-01-15 2005-07-28 Japan Science And Technology Agency 化学分析装置及び化学分析方法
FR2866493B1 (fr) 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
US7495031B2 (en) 2004-02-24 2009-02-24 Kao Corporation Process for producing an emulsion
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
US7048889B2 (en) 2004-03-23 2006-05-23 Lucent Technologies Inc. Dynamically controllable biological/chemical detectors having nanostructured surfaces
US20050226991A1 (en) 2004-04-07 2005-10-13 Hossainy Syed F Methods for modifying balloon of a catheter assembly
KR100583231B1 (ko) 2004-04-13 2006-05-26 한국과학기술연구원 물방울형 세포 부유액을 이용한 세포 분리 장치
JP2007536634A (ja) 2004-05-04 2007-12-13 フィッシャー−ローズマウント・システムズ・インコーポレーテッド プロセス制御システムのためのサービス指向型アーキテクチャ
US8974652B2 (en) 2004-05-28 2015-03-10 Board Of Regents, The University Of Texas System Programmable fluidic processors
FR2871076A1 (fr) 2004-06-04 2005-12-09 Univ Lille Sciences Tech Dispositif pour desorption par rayonnement laser incorporant une manipulation de l'echantillon liquide sous forme de gouttes individuelles permettant leur traitement chimique et biochimique
US7121998B1 (en) 2004-06-08 2006-10-17 Eurica Califorrniaa Vented microcradle for prenidial incubator
FR2872438B1 (fr) 2004-07-01 2006-09-15 Commissariat Energie Atomique Dispositif de deplacement et de traitement de volumes de liquide
US7693666B2 (en) 2004-07-07 2010-04-06 Rensselaer Polytechnic Institute Method, system, and program product for controlling chemical reactions in a digital microfluidic system
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
WO2006025982A2 (en) 2004-07-28 2006-03-09 University Of Rochester Rapid flow fractionation of particles combining liquid and particulate dielectrophoresis
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
US20060102477A1 (en) 2004-08-26 2006-05-18 Applera Corporation Electrowetting dispensing devices and related methods
JP4047314B2 (ja) 2004-09-07 2008-02-13 株式会社東芝 微細流路構造体
JP5885901B2 (ja) 2004-09-09 2016-03-16 アンスティテュート キュリー マイクロチャネルまたは他のマイクロ容器中でパケットを操作するためのデバイス
JP4185904B2 (ja) 2004-10-27 2008-11-26 株式会社日立ハイテクノロジーズ 液体搬送基板、分析システム、分析方法
FR2879946B1 (fr) 2004-12-23 2007-02-09 Commissariat Energie Atomique Dispositif de dispense de gouttes
US7458661B2 (en) 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
PL1859330T3 (pl) 2005-01-28 2013-01-31 Univ Duke Urządzenia i sposoby manipulacji kropelkami na płytkach obwodów drukowanych
US20060210443A1 (en) 2005-03-14 2006-09-21 Stearns Richard G Avoidance of bouncing and splashing in droplet-based fluid transport
FR2884437B1 (fr) 2005-04-19 2007-07-20 Commissariat Energie Atomique Dispositif et procede microfluidique de transfert de matiere entre deux phases immiscibles.
EP1885885A4 (en) 2005-05-11 2008-08-27 Nanolytics Inc METHOD OR DEVICE FOR CONDUCTING CHEMICAL OR BIOCHEMICAL REACTIONS AT MULTIPLE TEMPERATURES
JP2006317364A (ja) 2005-05-16 2006-11-24 Hitachi High-Technologies Corp 分注装置
US8481125B2 (en) 2005-05-21 2013-07-09 Advanced Liquid Logic Inc. Mitigation of biomolecular adsorption with hydrophilic polymer additives
JP2006329904A (ja) 2005-05-30 2006-12-07 Hitachi High-Technologies Corp 液体搬送デバイス及び分析システム
JP4500733B2 (ja) 2005-05-30 2010-07-14 株式会社日立ハイテクノロジーズ 化学分析装置
JP4969060B2 (ja) 2005-06-08 2012-07-04 株式会社日立ハイテクノロジーズ 自動分析装置
CN101252993A (zh) 2005-06-16 2008-08-27 精华微技有限公司 利用液滴驱动、搅拌和蒸发的生物传感器检测
FR2887305B1 (fr) 2005-06-17 2011-05-27 Commissariat Energie Atomique Dispositif de pompage par electromouillage et application aux mesures d'activite electrique
DE602005011870D1 (de) 2005-07-01 2009-01-29 Commissariat Energie Atomique Hydrophobe oberflächenbeschichtung mit geringer benetzungshysterese, aufbringungsverfahren dafür, mikrokomponente und verwendung
FR2888912B1 (fr) 2005-07-25 2007-08-24 Commissariat Energie Atomique Procede de commande d'une communication entre deux zones par electromouillage, dispositif comportant des zones isolables les unes des autres et procede de realisation d'un tel dispositif
US20070023292A1 (en) 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
US7556776B2 (en) 2005-09-08 2009-07-07 President And Fellows Of Harvard College Microfluidic manipulation of fluids and reactions
CA2623130C (en) 2005-09-21 2018-01-09 Luminex Corporation Methods and systems for image data processing
FR2890875B1 (fr) 2005-09-22 2008-02-22 Commissariat Energie Atomique Fabrication d'un systeme diphasique liquide/liquide ou gaz en micro-fluidique
US20070075922A1 (en) 2005-09-28 2007-04-05 Jessop Richard V Electronic display systems
US7344679B2 (en) 2005-10-14 2008-03-18 International Business Machines Corporation Method and apparatus for point of care osmolarity testing
CN101351270A (zh) 2005-10-22 2009-01-21 精华微技有限公司 从用于芯片微流控的液柱中抽取液滴
WO2007076023A2 (en) 2005-12-21 2007-07-05 Meso Scale Technologies, Llc Assay modules having assay reagents and methods of making and using same
EP3913375A1 (en) 2006-01-11 2021-11-24 Bio-Rad Laboratories, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US20070207513A1 (en) 2006-03-03 2007-09-06 Luminex Corporation Methods, Products, and Kits for Identifying an Analyte in a Sample
US8613889B2 (en) 2006-04-13 2013-12-24 Advanced Liquid Logic, Inc. Droplet-based washing
US8492168B2 (en) 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
WO2010006166A2 (en) 2008-07-09 2010-01-14 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8637317B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Method of washing beads
WO2007123908A2 (en) 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Droplet-based multiwell operations
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
US8658111B2 (en) * 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
ATE490971T1 (de) 2006-04-18 2010-12-15 Advanced Liquid Logic Inc Biochemie auf tröpfchenbasis
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
US8685754B2 (en) 2006-04-18 2014-04-01 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for immunoassays and washing
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7815871B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
US8470606B2 (en) 2006-04-18 2013-06-25 Duke University Manipulation of beads in droplets and methods for splitting droplets
US7816121B2 (en) * 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
WO2010042637A2 (en) 2008-10-07 2010-04-15 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7763471B2 (en) 2006-04-18 2010-07-27 Advanced Liquid Logic, Inc. Method of electrowetting droplet operations for protein crystallization
WO2010027894A2 (en) 2008-08-27 2010-03-11 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
EP2021103B1 (en) 2006-05-09 2017-07-12 Advanced Liquid Logic, Inc. Electrowetting droplet microactuator controlled via graphical user interface
US7822510B2 (en) 2006-05-09 2010-10-26 Advanced Liquid Logic, Inc. Systems, methods, and products for graphically illustrating and controlling a droplet actuator
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
WO2009026339A2 (en) 2007-08-20 2009-02-26 Advanced Liquid Logic, Inc. Modular droplet actuator drive
US8041463B2 (en) 2006-05-09 2011-10-18 Advanced Liquid Logic, Inc. Modular droplet actuator drive
US7939021B2 (en) 2007-05-09 2011-05-10 Advanced Liquid Logic, Inc. Droplet actuator analyzer with cartridge
EP2021113A2 (en) 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
WO2007146025A2 (en) 2006-06-06 2007-12-21 University Of Virginia Patent Foundation Capillary force actuator device and related method of applications
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US8128798B2 (en) 2006-07-10 2012-03-06 Hitachi High-Technologies Corporation Liquid transfer device
EP1905513A1 (en) 2006-09-13 2008-04-02 Institut Curie Methods and devices for sampling fluids
JP4901410B2 (ja) 2006-10-10 2012-03-21 シャープ株式会社 バックライト装置及び映像表示装置
WO2008055256A2 (en) 2006-11-02 2008-05-08 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
FR2909293B1 (fr) 2006-12-05 2011-04-22 Commissariat Energie Atomique Micro-dispositif de traitement d'echantillons liquides
EP2099930B1 (en) 2006-12-13 2015-02-18 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
EP2111554B1 (en) 2007-02-09 2013-05-08 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
WO2008101194A2 (en) 2007-02-15 2008-08-21 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
WO2008106678A1 (en) 2007-03-01 2008-09-04 Advanced Liquid Logic, Inc. Droplet actuator structures
CN101663403B (zh) 2007-03-05 2013-04-24 先进流体逻辑公司 基于微滴的过氧化氢测定
BRPI0808400A2 (pt) 2007-03-13 2014-07-08 Advanced Liquid Logic Inc Dispositivos atuadores de gotículas, configurações, e métodos para aperfeiçoar a detecção da absorbância.
EP2837692A1 (en) 2007-03-22 2015-02-18 Advanced Liquid Logic, Inc. Enzymatic assays for a droplet actuator
US8093062B2 (en) 2007-03-22 2012-01-10 Theodore Winger Enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil
WO2008116221A1 (en) 2007-03-22 2008-09-25 Advanced Liquid Logic, Inc. Bead sorting on a droplet actuator
US8202686B2 (en) 2007-03-22 2012-06-19 Advanced Liquid Logic, Inc. Enzyme assays for a droplet actuator
WO2008118831A2 (en) 2007-03-23 2008-10-02 Advanced Liquid Logic, Inc. Droplet actuator loading and target concentration
JP2010524002A (ja) 2007-04-10 2010-07-15 アドヴァンスト リキッド ロジック インコーポレイテッド 液滴分配装置および方法
US20100206094A1 (en) 2007-04-23 2010-08-19 Advanced Liquid Logic, Inc. Device and Method for Sample Collection and Concentration
US20100087012A1 (en) 2007-04-23 2010-04-08 Advanced Liquid Logic, Inc. Sample Collector and Processor
WO2008134153A1 (en) 2007-04-23 2008-11-06 Advanced Liquid Logic, Inc. Bead-based multiplexed analytical methods and instrumentation
US20080283414A1 (en) 2007-05-17 2008-11-20 Monroe Charles W Electrowetting devices
WO2009002920A1 (en) 2007-06-22 2008-12-31 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
CN101679932A (zh) 2007-06-27 2010-03-24 数字化生物系统 用于热交换化学过程的基于数字微流体的装置
US20110303542A1 (en) 2007-08-08 2011-12-15 Advanced Liquid Logic, Inc. Use of Additives for Enhancing Droplet Operations
US20100120130A1 (en) * 2007-08-08 2010-05-13 Advanced Liquid Logic, Inc. Droplet Actuator with Droplet Retention Structures
WO2009021233A2 (en) 2007-08-09 2009-02-12 Advanced Liquid Logic, Inc. Pcb droplet actuator fabrication
AU2008293652B2 (en) 2007-08-24 2013-02-21 Advanced Liquid Logic, Inc. Bead manipulations on a droplet actuator
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US8460528B2 (en) 2007-10-17 2013-06-11 Advanced Liquid Logic Inc. Reagent storage and reconstitution for a droplet actuator
US20100236928A1 (en) 2007-10-17 2010-09-23 Advanced Liquid Logic, Inc. Multiplexed Detection Schemes for a Droplet Actuator
US8454905B2 (en) 2007-10-17 2013-06-04 Advanced Liquid Logic Inc. Droplet actuator structures
EP2212683A4 (en) 2007-10-17 2011-08-31 Advanced Liquid Logic Inc MANIPULATION OF BEADS INTO DROPLETS
US20100236929A1 (en) 2007-10-18 2010-09-23 Advanced Liquid Logic, Inc. Droplet Actuators, Systems and Methods
US7621059B2 (en) 2007-10-18 2009-11-24 Oceaneering International, Inc. Underwater sediment evacuation system
WO2009076414A2 (en) 2007-12-10 2009-06-18 Advanced Liquid Logic, Inc. Droplet actuator configurations and methods
EP2237955A4 (en) 2007-12-23 2016-04-20 Advanced Liquid Logic Inc DROPLET FORMATION ACTUATOR CONFIGURATIONS, AND METHODS OF PERFORMING DROPLET FORMING OPERATIONS
US20110104725A1 (en) 2008-05-02 2011-05-05 Advanced Liquid Logic, Inc. Method of Effecting Coagulation in a Droplet
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US20110097763A1 (en) 2008-05-13 2011-04-28 Advanced Liquid Logic, Inc. Thermal Cycling Method
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
EP2286228B1 (en) 2008-05-16 2019-04-03 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for manipulating beads
FR2933713B1 (fr) 2008-07-11 2011-03-25 Commissariat Energie Atomique Procede et dispositif de manipulation et d'observation de gouttes de liquide
US20120261264A1 (en) 2008-07-18 2012-10-18 Advanced Liquid Logic, Inc. Droplet Operations Device
US8364315B2 (en) 2008-08-13 2013-01-29 Advanced Liquid Logic Inc. Methods, systems, and products for conducting droplet operations
WO2010077859A2 (en) 2008-12-15 2010-07-08 Advanced Liquid Logic, Inc. Nucleic acid amplification and sequencing on a droplet actuator
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
WO2011002957A2 (en) 2009-07-01 2011-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
WO2011020011A2 (en) 2009-08-13 2011-02-17 Advanced Liquid Logic, Inc. Droplet actuator and droplet-based techniques
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US8846414B2 (en) 2009-09-29 2014-09-30 Advanced Liquid Logic, Inc. Detection of cardiac markers on a droplet actuator
WO2011057197A2 (en) 2009-11-06 2011-05-12 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel electrophoresis and molecular analysis
EP2516669B1 (en) 2009-12-21 2016-10-12 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
US9248450B2 (en) 2010-03-30 2016-02-02 Advanced Liquid Logic, Inc. Droplet operations platform
US20130217113A1 (en) 2010-07-15 2013-08-22 Advanced Liquid Logic Inc. System for and methods of promoting cell lysis in droplet actuators
US20130293246A1 (en) 2010-11-17 2013-11-07 Advanced Liquid Logic Inc. Capacitance Detection in a Droplet Actuator
US20130018611A1 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic Inc Systems and Methods of Measuring Gap Height
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
US20130017544A1 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic Inc High Resolution Melting Analysis on a Droplet Actuator

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6911132B2 (en) * 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US20090280475A1 (en) * 2006-04-18 2009-11-12 Pollack Michael G Droplet-based pyrosequencing
WO2008038810A1 (en) * 2006-09-25 2008-04-03 Sumitomo Chemical Company, Limited Process for the preparation of 2-hydr0xy-4- (alkylthio or arylthio) butanoic acid by microbial conversion of 2-0x0-4- (alkylthio or arylthio) 1-butanol employing bacteria of the genus pseud0m0nas, rh0d0c0ccus, or bacillus
US20090311713A1 (en) * 2008-05-13 2009-12-17 Advanced Liquid Logic, Inc. Method of Detecting an Analyte
WO2012012090A2 (en) * 2010-06-30 2012-01-26 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
WO2012037308A2 (en) * 2010-09-16 2012-03-22 Advanced Liquid Logic, Inc. Droplet actuator systems, devices and methods

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