AU2009326306B2 - Process for the enzymatic production of cyclic diguanosine monophosphate employing a diguanylate cyclase comprising a mutated RXXD motif - Google Patents

Process for the enzymatic production of cyclic diguanosine monophosphate employing a diguanylate cyclase comprising a mutated RXXD motif Download PDF

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Publication number
AU2009326306B2
AU2009326306B2 AU2009326306A AU2009326306A AU2009326306B2 AU 2009326306 B2 AU2009326306 B2 AU 2009326306B2 AU 2009326306 A AU2009326306 A AU 2009326306A AU 2009326306 A AU2009326306 A AU 2009326306A AU 2009326306 B2 AU2009326306 B2 AU 2009326306B2
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AU
Australia
Prior art keywords
dgc
gmp
synthesis
mutant
modified
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Ceased
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AU2009326306A
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English (en)
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AU2009326306A1 (en
Inventor
Thomas Simon Ilg
Volker Spehr
Ralf Warrass
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Intervet International BV
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Intervet International BV
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Publication of AU2009326306A1 publication Critical patent/AU2009326306A1/en
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Publication of AU2009326306B2 publication Critical patent/AU2009326306B2/en
Ceased legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/36—Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C07H19/207—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids the phosphoric or polyphosphoric acids being esterified by a further hydroxylic compound, e.g. flavine adenine dinucleotide or nicotinamide-adenine dinucleotide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2009326306A 2008-12-09 2009-12-07 Process for the enzymatic production of cyclic diguanosine monophosphate employing a diguanylate cyclase comprising a mutated RXXD motif Ceased AU2009326306B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12092508P 2008-12-09 2008-12-09
EP08171026 2008-12-09
EP08171026.1 2008-12-09
US61/120,925 2008-12-09
PCT/EP2009/066492 WO2010066666A1 (en) 2008-12-09 2009-12-07 Process for the enzymatic production of cyclic diguanosine monophosphate employing a diguanylate cyclase comprising a mutated rxxd motif

Publications (2)

Publication Number Publication Date
AU2009326306A1 AU2009326306A1 (en) 2010-06-17
AU2009326306B2 true AU2009326306B2 (en) 2012-12-13

Family

ID=40602165

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2009326306A Ceased AU2009326306B2 (en) 2008-12-09 2009-12-07 Process for the enzymatic production of cyclic diguanosine monophosphate employing a diguanylate cyclase comprising a mutated RXXD motif

Country Status (12)

Country Link
US (1) US20110288286A1 (https=)
EP (1) EP2376634A1 (https=)
JP (1) JP2012511313A (https=)
KR (1) KR20110099111A (https=)
CN (1) CN102245774A (https=)
AR (1) AR074495A1 (https=)
AU (1) AU2009326306B2 (https=)
BR (1) BRPI0923296A2 (https=)
CA (1) CA2747127A1 (https=)
TW (1) TW201033367A (https=)
UY (1) UY32307A (https=)
WO (1) WO2010066666A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013129427A1 (ja) 2012-02-29 2013-09-06 ヤマサ醤油株式会社 cyclic di-GMPの実践的酵素合成法
CN113122593A (zh) * 2019-12-31 2021-07-16 安徽古特生物科技有限公司 一种利用多聚磷酸盐制备核苷三磷酸和脱氧核苷三磷酸的方法
CN111647611A (zh) * 2020-07-02 2020-09-11 杭州美亚药业股份有限公司 一种双鸟苷酸环化酶改造基因、编码蛋白、工程菌及其构建方法和应用
CN117701596A (zh) * 2023-12-08 2024-03-15 广东省科学院微生物研究所(广东省微生物分析检测中心) 一种大量合成天然环二鸟苷酸的基因工程菌及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6037145A (en) * 1994-09-07 2000-03-14 Suntory Limited Process for production of protein
JP3995279B2 (ja) * 1994-09-07 2007-10-24 アスビオファーマ株式会社 蛋白の製造方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHRISTEN, B. et al., '2006, The Journal of Biological Chemistry, vol. 281, no. 42, pages 32015-32024 *
CHRISTEN, M., 'Mechanisms of cyclic-di-GMP signaling: insight into the biochemistry, signal transduction and regulation of the bacterial second cyclic-di-GMP', 2007, PhD Thesis *
EMBL DATABASE Accession number B4EM14, 2008 *

Also Published As

Publication number Publication date
US20110288286A1 (en) 2011-11-24
JP2012511313A (ja) 2012-05-24
CA2747127A1 (en) 2010-06-17
BRPI0923296A2 (pt) 2015-08-11
EP2376634A1 (en) 2011-10-19
WO2010066666A1 (en) 2010-06-17
AU2009326306A1 (en) 2010-06-17
UY32307A (es) 2010-02-26
KR20110099111A (ko) 2011-09-06
CN102245774A (zh) 2011-11-16
AR074495A1 (es) 2011-01-19
TW201033367A (en) 2010-09-16

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