AU2002227311A1 - Methods and compositions for highly efficient production of heterologous proteins in yeast - Google Patents

Methods and compositions for highly efficient production of heterologous proteins in yeast

Info

Publication number
AU2002227311A1
AU2002227311A1 AU2002227311A AU2731102A AU2002227311A1 AU 2002227311 A1 AU2002227311 A1 AU 2002227311A1 AU 2002227311 A AU2002227311 A AU 2002227311A AU 2731102 A AU2731102 A AU 2731102A AU 2002227311 A1 AU2002227311 A1 AU 2002227311A1
Authority
AU
Australia
Prior art keywords
yeast
cell
pmt
gene
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002227311A
Other languages
English (en)
Inventor
Davis T. W. Ng
Shilpa Vashist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Penn State Research Foundation
Original Assignee
Penn State Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Penn State Research Foundation filed Critical Penn State Research Foundation
Publication of AU2002227311A1 publication Critical patent/AU2002227311A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2002227311A 2000-12-05 2001-12-05 Methods and compositions for highly efficient production of heterologous proteins in yeast Abandoned AU2002227311A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25137400P 2000-12-05 2000-12-05
US60251374 2000-12-05
PCT/US2001/047319 WO2002046437A2 (en) 2000-12-05 2001-12-05 Methods and compositions for highly efficient production of heterologous proteins in yeast

Publications (1)

Publication Number Publication Date
AU2002227311A1 true AU2002227311A1 (en) 2002-06-18

Family

ID=22951679

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002227311A Abandoned AU2002227311A1 (en) 2000-12-05 2001-12-05 Methods and compositions for highly efficient production of heterologous proteins in yeast

Country Status (10)

Country Link
US (1) US20020068325A1 (zh)
EP (1) EP1379667A2 (zh)
JP (1) JP2005515749A (zh)
CN (1) CN1592786A (zh)
AU (1) AU2002227311A1 (zh)
BR (1) BR0115912A (zh)
CA (1) CA2431013A1 (zh)
MX (1) MXPA03004853A (zh)
RU (1) RU2003120093A (zh)
WO (1) WO2002046437A2 (zh)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0315943A (pt) * 2002-12-20 2005-09-13 Unilever Nv Método para a produção de uma proteìna anticongelante e composição
CN101365783B (zh) * 2005-11-15 2013-07-17 格利科菲公司 具有降低的o-糖基化的糖蛋白的产生
AU2007250735A1 (en) * 2006-05-16 2007-11-22 Daiichi Sankyo Company, Limited Method for high-level secretory production of protein
WO2008053018A2 (en) * 2006-11-02 2008-05-08 Dsm Ip Assets B.V. Improved production of secreted proteins by filamentous fungi
WO2009048067A1 (ja) * 2007-10-09 2009-04-16 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute 糖鎖を持たない遺伝子組換え血液凝固第x因子およびその製法
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
JP2011514157A (ja) * 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
JP5731827B2 (ja) * 2008-03-03 2015-06-10 グライコフィ， インコーポレイテッド 下等真核生物中での組換えタンパク質の表面ディスプレイ
US8067339B2 (en) 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
JP2011530311A (ja) 2008-08-12 2011-12-22 グライコフィ， インコーポレイテッド タンパク質製造のための改良されたベクターおよび酵母株：Ｃａ２＋ＡＴＰアーゼ過剰発現
WO2010038802A1 (ja) 2008-10-01 2010-04-08 旭硝子株式会社 宿主、形質転換体およびその製造方法、ならびにｏ－グリコシド型糖鎖含有異種蛋白質の製造方法
US9120871B2 (en) 2009-01-23 2015-09-01 Novo Nordisk A/S Process for preparing FGF21 with low degree of O-glycosylation
WO2011088059A1 (en) 2010-01-12 2011-07-21 Whitehead Institute For Biomedical Research Yeast cells expressing amyloid beta and uses therefor
CN102791730A (zh) * 2010-01-22 2012-11-21 诺沃—诺迪斯克有限公司 低度o-糖基化的fgf21的制备方法
ES2738593T3 (es) 2012-12-10 2020-01-23 Seikagaku Kogyo Co Ltd Factor C recombinante novedoso y método para producir el mismo, y método para medir endotoxina
CN103275981B (zh) * 2013-05-27 2015-01-28 华南理工大学 丝状真菌蛋白分泌压力反馈调控元件与抗反馈抑制的启动子、质粒及制备方法和转化细胞
WO2015001049A1 (en) 2013-07-04 2015-01-08 Novartis Ag O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof
CN104630174B (zh) * 2013-11-07 2019-09-27 丰益(上海)生物技术研发中心有限公司 磷脂酶c突变体及其用途
KR102342622B1 (ko) 2014-06-13 2021-12-22 화이트헤드 인스티튜트 포 바이오메디칼 리서치 아밀로이드 베타 발현 작제물 및 이에 대한 용도
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
CN111500561B (zh) * 2020-05-08 2022-12-02 江南大学 一种提高胞内普鲁兰酶提取效率的方法
CN117777276A (zh) * 2024-02-23 2024-03-29 北京国科星联科技有限公司 一种促进马克斯克鲁维酵母分泌表达人乳铁蛋白的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4226971C2 (de) * 1992-08-14 1997-01-16 Widmar Prof Dr Tanner Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte
SE9301583D0 (sv) * 1993-05-07 1993-05-07 Kabi Pharmacia Ab Yeast strain and methods for expressing heterologous proteins in yeast
CA2232536A1 (en) * 1998-03-19 1999-09-19 Howard Bussey Antifungal drug screening using saccharomyces cerevisiae mannosyltransferase encoding genes

Also Published As

Publication number Publication date
WO2002046437A3 (en) 2003-11-06
WO2002046437A2 (en) 2002-06-13
EP1379667A2 (en) 2004-01-14
JP2005515749A (ja) 2005-06-02
CA2431013A1 (en) 2002-06-13
US20020068325A1 (en) 2002-06-06
CN1592786A (zh) 2005-03-09
RU2003120093A (ru) 2005-01-27
BR0115912A (pt) 2005-10-18
MXPA03004853A (es) 2005-02-14

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