BR0115912A - Métodos e composições para a produção altamente eficiente de proteìnas heterólogas em levedura - Google Patents

Métodos e composições para a produção altamente eficiente de proteìnas heterólogas em levedura

Info

Publication number
BR0115912A
BR0115912A BR0115912-7A BR0115912A BR0115912A BR 0115912 A BR0115912 A BR 0115912A BR 0115912 A BR0115912 A BR 0115912A BR 0115912 A BR0115912 A BR 0115912A
Authority
BR
Brazil
Prior art keywords
proteins
compositions
highly efficient
efficient production
methods
Prior art date
Application number
BR0115912-7A
Other languages
English (en)
Inventor
Davis T W Ng
Shilpa Vashist
Original Assignee
Penn State Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Penn State Res Found filed Critical Penn State Res Found
Publication of BR0115912A publication Critical patent/BR0115912A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

"MéTODOS E COMPOSIçõES PARA A PRODUçãO ALTAMENTE EFICIENTE DE PROTEìNAS HETERóLOGAS EM LEVEDURA". A invenção proporciona métodos e composições para a produção altamente eficiente de proteínas heterólogas em levedura e outros fungos, superando os problemas anteriores associados com a incapacidade destas proteínas de duplicarem-se apropriadamente. De acordo com a invenção, o mecanismo de controle de qualidade empregado pelos fungos, que retorna as proteínas duplicadas erroneamente para o citossol para a degradação, é manipulado de modo que estas proteínas sejam em vez disso secretadas.
BR0115912-7A 2000-12-05 2001-12-05 Métodos e composições para a produção altamente eficiente de proteìnas heterólogas em levedura BR0115912A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25137400P 2000-12-05 2000-12-05
PCT/US2001/047319 WO2002046437A2 (en) 2000-12-05 2001-12-05 Methods and compositions for highly efficient production of heterologous proteins in yeast

Publications (1)

Publication Number Publication Date
BR0115912A true BR0115912A (pt) 2005-10-18

Family

ID=22951679

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0115912-7A BR0115912A (pt) 2000-12-05 2001-12-05 Métodos e composições para a produção altamente eficiente de proteìnas heterólogas em levedura

Country Status (10)

Country Link
US (1) US20020068325A1 (pt)
EP (1) EP1379667A2 (pt)
JP (1) JP2005515749A (pt)
CN (1) CN1592786A (pt)
AU (1) AU2002227311A1 (pt)
BR (1) BR0115912A (pt)
CA (1) CA2431013A1 (pt)
MX (1) MXPA03004853A (pt)
RU (1) RU2003120093A (pt)
WO (1) WO2002046437A2 (pt)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100383251C (zh) * 2002-12-20 2008-04-23 荷兰联合利华有限公司 抗冻蛋白的制备
AU2006316838B2 (en) 2005-11-15 2012-04-12 Glycofi, Inc Production of glycoproteins with reduced O-glycosylation
AU2007250735A1 (en) * 2006-05-16 2007-11-22 Daiichi Sankyo Company, Limited Method for high-level secretory production of protein
US8389269B2 (en) * 2006-11-02 2013-03-05 Dsm Ip Assets B.V. Production of secreted proteins by filamentous fungi
US8173777B2 (en) 2007-10-09 2012-05-08 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Recombinant Factor X with no glycosylation and method for preparing the same
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
CN101945998B (zh) * 2008-02-20 2013-09-18 格利科菲公司 用于蛋白生产的载体和酵母菌株
WO2009111183A1 (en) 2008-03-03 2009-09-11 Glycofi, Inc. Surface display of recombinant proteins in lower eukaryotes
US8067339B2 (en) * 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
AU2009282228A1 (en) 2008-08-12 2010-02-18 Glycofi, Inc. Improved vectors and yeast strains for protein production: Ca2+ ATPase overexpression
JP5652206B2 (ja) 2008-10-01 2015-01-14 旭硝子株式会社 宿主、形質転換体およびその製造方法、ならびにｏ−グリコシド型糖鎖含有異種蛋白質の製造方法
US9120871B2 (en) 2009-01-23 2015-09-01 Novo Nordisk A/S Process for preparing FGF21 with low degree of O-glycosylation
WO2011088059A1 (en) * 2010-01-12 2011-07-21 Whitehead Institute For Biomedical Research Yeast cells expressing amyloid beta and uses therefor
ES2543634T3 (es) * 2010-01-22 2015-08-20 Novo Nordisk A/S Proceso para preparación de FGF-21 con grado de O-glicosilación bajo
ES2738593T3 (es) * 2012-12-10 2020-01-23 Seikagaku Kogyo Co Ltd Factor C recombinante novedoso y método para producir el mismo, y método para medir endotoxina
CN103275981B (zh) * 2013-05-27 2015-01-28 华南理工大学 丝状真菌蛋白分泌压力反馈调控元件与抗反馈抑制的启动子、质粒及制备方法和转化细胞
KR102111078B1 (ko) 2013-07-04 2020-05-18 노파르티스 아게 O-만노실트랜스퍼라제 결핍 사상균 세포 및 이의 이용 방법
CN104630174B (zh) * 2013-11-07 2019-09-27 丰益(上海)生物技术研发中心有限公司 磷脂酶c突变体及其用途
JP6655557B2 (ja) 2014-06-13 2020-02-26 ホワイトヘッド インスティテュート フォー バイオメディカル リサーチ アミロイドベータ発現構築物およびその使用
CA2954974A1 (en) 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
CN111500561B (zh) * 2020-05-08 2022-12-02 江南大学 一种提高胞内普鲁兰酶提取效率的方法
CN117777276A (zh) * 2024-02-23 2024-03-29 北京国科星联科技有限公司 一种促进马克斯克鲁维酵母分泌表达人乳铁蛋白的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4226971C2 (de) * 1992-08-14 1997-01-16 Widmar Prof Dr Tanner Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte
SE9301583D0 (sv) * 1993-05-07 1993-05-07 Kabi Pharmacia Ab Yeast strain and methods for expressing heterologous proteins in yeast
CA2232536A1 (en) * 1998-03-19 1999-09-19 Howard Bussey Antifungal drug screening using saccharomyces cerevisiae mannosyltransferase encoding genes

Also Published As

Publication number Publication date
JP2005515749A (ja) 2005-06-02
RU2003120093A (ru) 2005-01-27
CA2431013A1 (en) 2002-06-13
US20020068325A1 (en) 2002-06-06
AU2002227311A1 (en) 2002-06-18
WO2002046437A2 (en) 2002-06-13
MXPA03004853A (es) 2005-02-14
EP1379667A2 (en) 2004-01-14
CN1592786A (zh) 2005-03-09
WO2002046437A3 (en) 2003-11-06

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]