AU2001287695B2 - Method for purifying recombinant proteins expressed as insoluble aggregates - Google Patents

Method for purifying recombinant proteins expressed as insoluble aggregates Download PDF

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Publication number
AU2001287695B2
AU2001287695B2 AU2001287695A AU2001287695A AU2001287695B2 AU 2001287695 B2 AU2001287695 B2 AU 2001287695B2 AU 2001287695 A AU2001287695 A AU 2001287695A AU 2001287695 A AU2001287695 A AU 2001287695A AU 2001287695 B2 AU2001287695 B2 AU 2001287695B2
Authority
AU
Australia
Prior art keywords
recombinant
purification
solution
chromatography
allergen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2001287695A
Other languages
English (en)
Other versions
AU2001287695A1 (en
Inventor
Oliver Cromwell
Helmut Fiebig
Roland Suck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of AU2001287695A1 publication Critical patent/AU2001287695A1/en
Application granted granted Critical
Publication of AU2001287695B2 publication Critical patent/AU2001287695B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2001287695A 2000-09-08 2001-08-18 Method for purifying recombinant proteins expressed as insoluble aggregates Ceased AU2001287695B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10044360.5 2000-09-08
DE10044360A DE10044360A1 (de) 2000-09-08 2000-09-08 Verfahren zur Aufreinigung von rekombinanten als unlösliche Aggregate exprimierte Proteinen
PCT/EP2001/009552 WO2002020559A1 (de) 2000-09-08 2001-08-18 Verfahren zur aufreinigung von rekombinanten als unlösliche aggregate exprimierte proteinen

Publications (2)

Publication Number Publication Date
AU2001287695A1 AU2001287695A1 (en) 2002-06-13
AU2001287695B2 true AU2001287695B2 (en) 2006-10-05

Family

ID=7655449

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2001287695A Ceased AU2001287695B2 (en) 2000-09-08 2001-08-18 Method for purifying recombinant proteins expressed as insoluble aggregates
AU8769501A Pending AU8769501A (en) 2000-09-08 2001-08-18 Method for purifying recombinant proteins expressed as insoluble aggregates

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU8769501A Pending AU8769501A (en) 2000-09-08 2001-08-18 Method for purifying recombinant proteins expressed as insoluble aggregates

Country Status (14)

Country Link
US (1) US6900034B2 (https=)
EP (1) EP1315740B1 (https=)
JP (1) JP5174312B2 (https=)
CN (1) CN1468255A (https=)
AT (1) ATE443712T1 (https=)
AU (2) AU2001287695B2 (https=)
CA (1) CA2421438C (https=)
DE (2) DE10044360A1 (https=)
ES (1) ES2333939T3 (https=)
HU (1) HUP0303554A2 (https=)
PL (1) PL206158B1 (https=)
PT (1) PT1315740E (https=)
RU (1) RU2299214C2 (https=)
WO (1) WO2002020559A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2333951T5 (es) * 2002-02-27 2017-11-16 Merck Patent Gmbh Método para producir alergenos principales rBet v 1 hipoalergénicos de abedul
CA2487673C (en) * 2003-12-02 2010-11-02 F. Hoffmann-La Roche Ag Improved method for the recombinant production and purification of protein kinases
EP2323485B1 (en) * 2008-08-08 2013-10-16 Janssen Pharmaceutica, N.V. Anti-allergen combinations of calcium and lanthanum salts
PL2721051T3 (pl) 2011-06-15 2018-05-30 Asit Biotech S.A. Sposób wytwarzania hydrolizowanych alergenów
CN104558100A (zh) * 2015-02-04 2015-04-29 吉林农业大学 包涵体的预处理方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US5101018A (en) * 1989-06-12 1992-03-31 International Minerals & Chemical Corp. Method for recovering recombinant proteins
ATE113060T1 (de) 1989-12-05 1994-11-15 American Cyanamid Co Methode zur auflösung und naturation von somatotropin unter verwendung von einer niedrigen harnstoffkonzentration.
US5912327A (en) 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
SE9703531D0 (sv) * 1997-09-30 1997-09-30 Rudolf Valenta Non-anaphlactic forms of allergens and their use

Also Published As

Publication number Publication date
DE50115119D1 (de) 2009-11-05
DE10044360A1 (de) 2002-03-21
JP2004508384A (ja) 2004-03-18
JP5174312B2 (ja) 2013-04-03
EP1315740B1 (de) 2009-09-23
AU8769501A (en) 2002-03-22
WO2002020559A1 (de) 2002-03-14
CA2421438A1 (en) 2003-03-06
PL359713A1 (en) 2004-09-06
ATE443712T1 (de) 2009-10-15
CN1468255A (zh) 2004-01-14
US20030170815A1 (en) 2003-09-11
PT1315740E (pt) 2009-12-29
CA2421438C (en) 2010-10-26
RU2299214C2 (ru) 2007-05-20
ES2333939T3 (es) 2010-03-03
EP1315740A1 (de) 2003-06-04
HUP0303554A2 (hu) 2004-03-01
US6900034B2 (en) 2005-05-31
PL206158B1 (pl) 2010-07-30

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Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired