ATE85076T1 - Verfahren zur mikroinjektion in lebende zellen und vorrichtung zur durchfuehrung des verfahrens. - Google Patents

Verfahren zur mikroinjektion in lebende zellen und vorrichtung zur durchfuehrung des verfahrens.

Info

Publication number
ATE85076T1
ATE85076T1 AT89102612T AT89102612T ATE85076T1 AT E85076 T1 ATE85076 T1 AT E85076T1 AT 89102612 T AT89102612 T AT 89102612T AT 89102612 T AT89102612 T AT 89102612T AT E85076 T1 ATE85076 T1 AT E85076T1
Authority
AT
Austria
Prior art keywords
needle
cell
plane
stage
microinjection
Prior art date
Application number
AT89102612T
Other languages
German (de)
English (en)
Inventor
Guenther Dipl Bio Dr Bertholdt
Original Assignee
Eppendorf Geraetebau Netheler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Geraetebau Netheler filed Critical Eppendorf Geraetebau Netheler
Application granted granted Critical
Publication of ATE85076T1 publication Critical patent/ATE85076T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT89102612T 1988-03-15 1989-02-16 Verfahren zur mikroinjektion in lebende zellen und vorrichtung zur durchfuehrung des verfahrens. ATE85076T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3808531A DE3808531C1 (enExample) 1988-03-15 1988-03-15
EP89102612A EP0332864B1 (de) 1988-03-15 1989-02-16 Verfahren zur Mikroinjektion in lebende Zellen und Vorrichtung zur Durchführung des Verfahrens

Publications (1)

Publication Number Publication Date
ATE85076T1 true ATE85076T1 (de) 1993-02-15

Family

ID=6349731

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89102612T ATE85076T1 (de) 1988-03-15 1989-02-16 Verfahren zur mikroinjektion in lebende zellen und vorrichtung zur durchfuehrung des verfahrens.

Country Status (6)

Country Link
US (1) US5114854A (enExample)
EP (1) EP0332864B1 (enExample)
JP (1) JPH0646943B2 (enExample)
AT (1) ATE85076T1 (enExample)
DE (1) DE3808531C1 (enExample)
ES (1) ES2037889T3 (enExample)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10136481A1 (de) * 2001-07-27 2003-02-20 Leica Microsystems Anordnung zum Mikromanipulieren von biologischen Objekten
US5413924A (en) * 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
US5344610A (en) * 1993-02-03 1994-09-06 Eastman Kodak Company Aspirator probe with long pivot arm to minimize tip flick
DE4401076C2 (de) * 1994-01-15 1998-12-03 Eppendorf Geraetebau Netheler Vorrichtung zur Injektion von Flüssigkeiten in biologische Zellen
DE19629141A1 (de) 1996-07-19 1998-04-16 Bayer Ag Verfahren und Vorrichtung zum Screening von Molekülen bezüglich ihres individuellen Bindungsverhaltens zu mindestens einem vorgegebenen Ligand
DE19714987C1 (de) * 1997-04-10 1998-09-24 Deutsches Krebsforsch Vorrichtung zur Isolation von Partikeln, insbesondere von Zellen
DE19721084A1 (de) * 1997-05-20 1998-11-26 Max Planck Gesellschaft Mikroinjektion
US5877008A (en) * 1997-07-22 1999-03-02 Lockheed Martin Energy Research Corporation Microinjector for blasocysts
US6410057B1 (en) 1997-12-12 2002-06-25 Samyang Corporation Biodegradable mixed polymeric micelles for drug delivery
AR018528A1 (es) 1997-12-12 2001-11-28 Expression Genetics Inc Mezcla polimerica biodegradable de micelas para transferencia genetica
US6759014B2 (en) * 2001-01-26 2004-07-06 Symyx Technologies, Inc. Apparatus and methods for parallel processing of multiple reaction mixtures
US6913934B2 (en) * 1998-08-13 2005-07-05 Symyx Technologies, Inc. Apparatus and methods for parallel processing of multiple reaction mixtures
CA2406572A1 (en) 1999-04-23 2000-11-02 Monica Palacios-Boyce Microelectromechanical devices useful for manipulating cells or embryos, kits thereof, methods of making same, and methods of use thereof
JP3442357B2 (ja) * 2000-08-25 2003-09-02 株式会社日立製作所 両生類卵母細胞試料導入装置、両生類卵母細胞試料導入システム、両生類卵母細胞試料導入方法、両生類卵母細胞の製造方法、両生類卵母細胞及びそれを販売又は譲渡する方法、スクリーニング用のセンサーとして用いる方法、容器、及び解析方法
DE10136572B4 (de) * 2001-07-27 2005-11-17 Eppendorf Ag Vorrichtung zur optisch kontrollierten Mikromanipulation von biologischen Objekten
EP1544286A4 (en) * 2002-08-08 2007-06-27 Univ Tokyo Nat Univ Corp WORKING ON A SINGLE CELL UNDERSTANDING ROBOT
KR100475098B1 (ko) * 2002-11-12 2005-03-11 한국과학기술연구원 단일 셀 조작을 위한 바이오 자동 조작 장치
MXPA06001719A (es) * 2003-08-11 2006-05-19 Codexis Inc Polipeptidos de glucosa deshidrogenasa mejorados, y polinucleotidos relacionados.
JP4504082B2 (ja) 2004-04-28 2010-07-14 富士通株式会社 液体注入装置
JP4456429B2 (ja) * 2004-07-27 2010-04-28 富士通株式会社 インジェクション装置
DE102004053596B4 (de) * 2004-11-05 2006-10-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtungen zur Bearbeitung einzelner biologischer Zellen
JP4894199B2 (ja) * 2005-02-17 2012-03-14 富士通株式会社 物質注入装置
US20060246571A1 (en) * 2005-04-29 2006-11-02 Fujitsu Limited Apparatus and method for automatically producing substance-introduced particles
US7491669B2 (en) * 2006-02-07 2009-02-17 Crystal Clear Technologies, Inc. Adsorbent with multiple layers
JP2007166981A (ja) * 2005-12-22 2007-07-05 Fujitsu Ltd 注入装置及び方法
US8497119B1 (en) 2010-12-15 2013-07-30 Paul J. Taylor Method and device for performing micro-operations on a vesicular object
DE102011012993B3 (de) * 2011-03-04 2012-07-12 Otto-Von-Guericke-Universität Magdeburg Integriertes Greif- und Zuführsystem für Anwendungen in der Mikro- und Nanotechnik zur Manipulation von Mikro- und Nano-Objekten und Verfahren zur Durchführung der Manipulation
KR101387422B1 (ko) * 2012-06-13 2014-04-21 듀라케미 (주) 관의 내.외부 방식도장에 의해 결합된 패킹 일체형 관
GB2508906A (en) * 2012-12-14 2014-06-18 Life Science Methods Bv Frame element for sample injection with optical control means
KR102649456B1 (ko) 2016-10-31 2024-03-19 미코노스 리미티드 생체 세포 주입을 위한 니들 매니퓰레이터들의 어레이
WO2018144814A1 (en) 2017-02-03 2018-08-09 Infinitesimal Llc Electroporation system with micromanipulator and probe
US12084340B2 (en) 2019-06-13 2024-09-10 Mekonos, Inc. Micro-electro-mechanical-system structures and applications thereof
US12233406B2 (en) 2021-08-04 2025-02-25 City University Of Hong Kong Automated system for high-throughput microinjection of adherent cells
GB202301403D0 (en) * 2023-01-31 2023-03-15 Francis Crick Institute Ltd Systems and method for examining biological material

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU675359A1 (en) * 1977-04-06 1979-07-25 Inst Biologicheskoi Fiz Method of obtaining sub-cell structures
JPS54158945A (en) * 1978-06-05 1979-12-15 Olympus Optical Co Ltd Micro-manipulator
DE3204040A1 (de) * 1982-02-05 1983-08-11 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg Verfahren und vorrichtung zur injektion von sehr kleinen probenmengen in zellen
DE3390499C2 (de) * 1983-06-30 1986-11-06 Institut Biochimii I Fiziologii Mikroorganizmov Akademii Nauk Sssr Verfahren zur Durchf}hrung von Mikrooperationen anZelten und Einrichtung zu dessen Verwirklichung
JPS60118473A (ja) * 1983-11-29 1985-06-25 オリンパス光学工業株式会社 マイクロマニピユレ−タ
JPS60205411A (ja) * 1984-03-29 1985-10-17 Olympus Optical Co Ltd 倒立型顕微鏡
DE3718066A1 (de) * 1987-05-29 1988-12-08 Zeiss Carl Fa Verfahren zur mikroinjektion in zellen bzw. zum absaugen aus einzelnen zellen oder ganzer zellen aus zellkulturen

Also Published As

Publication number Publication date
JPH0646943B2 (ja) 1994-06-22
US5114854A (en) 1992-05-19
JPH02119770A (ja) 1990-05-07
EP0332864B1 (de) 1993-01-27
EP0332864A3 (en) 1990-07-18
DE3808531C1 (enExample) 1989-07-13
ES2037889T3 (es) 1993-07-01
EP0332864A2 (de) 1989-09-20

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Legal Events

Date Code Title Description
EELA Cancelled due to lapse of time