ATE544771T1 - Fluoreszent quenching detektion reagenzien und verfahren - Google Patents

Fluoreszent quenching detektion reagenzien und verfahren

Info

Publication number
ATE544771T1
ATE544771T1 AT00984069T AT00984069T ATE544771T1 AT E544771 T1 ATE544771 T1 AT E544771T1 AT 00984069 T AT00984069 T AT 00984069T AT 00984069 T AT00984069 T AT 00984069T AT E544771 T1 ATE544771 T1 AT E544771T1
Authority
AT
Austria
Prior art keywords
substituted
quencher
fluorophore
moiety
phenyldiazenyl
Prior art date
Application number
AT00984069T
Other languages
German (de)
English (en)
Inventor
Michael Reed
Eugeny Lukhtanov
Alexander Gall
Robert Dempcy
Yevgeniy Belousov
Original Assignee
Epoch Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epoch Biosciences Inc filed Critical Epoch Biosciences Inc
Application granted granted Critical
Publication of ATE544771T1 publication Critical patent/ATE544771T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C245/00—Compounds containing chains of at least two nitrogen atoms with at least one nitrogen-to-nitrogen multiple bond
    • C07C245/02—Azo compounds, i.e. compounds having the free valencies of —N=N— groups attached to different atoms, e.g. diazohydroxides
    • C07C245/06—Azo compounds, i.e. compounds having the free valencies of —N=N— groups attached to different atoms, e.g. diazohydroxides with nitrogen atoms of azo groups bound to carbon atoms of six-membered aromatic rings
    • C07C245/08—Azo compounds, i.e. compounds having the free valencies of —N=N— groups attached to different atoms, e.g. diazohydroxides with nitrogen atoms of azo groups bound to carbon atoms of six-membered aromatic rings with the two nitrogen atoms of azo groups bound to carbon atoms of six-membered aromatic rings, e.g. azobenzene
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/22—Amides of acids of phosphorus
    • C07F9/24—Esteramides
    • C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/2408—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
    • C07F9/572—Five-membered rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
    • C07F9/6552—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a six-membered ring
    • C07F9/65522—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a six-membered ring condensed with carbocyclic rings or carbocyclic ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B29/00—Monoazo dyes prepared by diazotising and coupling
    • C09B29/06—Monoazo dyes prepared by diazotising and coupling from coupling components containing amino as the only directing group
    • C09B29/08—Amino benzenes
    • C09B29/0805—Amino benzenes free of acid groups
    • C09B29/0807—Amino benzenes free of acid groups characterised by the amino group
    • C09B29/0809—Amino benzenes free of acid groups characterised by the amino group substituted amino group
    • C09B29/0811—Amino benzenes free of acid groups characterised by the amino group substituted amino group further substituted alkylamino, alkenylamino, alkynylamino, cycloalkylamino aralkylamino or arylamino
    • C09B29/0813—Amino benzenes free of acid groups characterised by the amino group substituted amino group further substituted alkylamino, alkenylamino, alkynylamino, cycloalkylamino aralkylamino or arylamino substituted by OH, O-C(=X)-R, O-C(=X)-X-R, O-R (X being O,S,NR; R being hydrocarbonyl)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
AT00984069T 1999-12-08 2000-12-08 Fluoreszent quenching detektion reagenzien und verfahren ATE544771T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/457,616 US6727356B1 (en) 1999-12-08 1999-12-08 Fluorescent quenching detection reagents and methods
PCT/US2000/033333 WO2001042505A2 (en) 1999-12-08 2000-12-08 Fluorescent quenching detection reagents and methods

Publications (1)

Publication Number Publication Date
ATE544771T1 true ATE544771T1 (de) 2012-02-15

Family

ID=23817445

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00984069T ATE544771T1 (de) 1999-12-08 2000-12-08 Fluoreszent quenching detektion reagenzien und verfahren

Country Status (8)

Country Link
US (5) US6727356B1 (US06727356-20040427-C00046.png)
EP (1) EP1235938B1 (US06727356-20040427-C00046.png)
JP (2) JP5019688B2 (US06727356-20040427-C00046.png)
AT (1) ATE544771T1 (US06727356-20040427-C00046.png)
AU (1) AU782204B2 (US06727356-20040427-C00046.png)
CA (1) CA2396795A1 (US06727356-20040427-C00046.png)
ES (1) ES2381861T3 (US06727356-20040427-C00046.png)
WO (1) WO2001042505A2 (US06727356-20040427-C00046.png)

Families Citing this family (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7715989B2 (en) * 1998-04-03 2010-05-11 Elitech Holding B.V. Systems and methods for predicting oligonucleotide melting temperature (TmS)
US20040081959A9 (en) * 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6727356B1 (en) * 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
EP1944310A3 (en) 2000-03-01 2008-08-06 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
AU2001243403A1 (en) 2000-03-01 2001-09-12 Epoch Biosiences, Inc. Modified oligonucleotides for mismatch discrimination
US7019129B1 (en) * 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
EP1295941B1 (en) * 2000-06-27 2009-12-16 National Institute of Advanced Industrial Science and Technology Novel nucleic acid probes and method of assaying nucleic acid by using the same
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7101999B2 (en) 2000-09-06 2006-09-05 Evotec Ag Oxazine derivatives
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US20030082547A1 (en) 2001-08-27 2003-05-01 Ewing Gregory J. Non-fluorescent quencher compounds and biomolecular assays
US6902900B2 (en) * 2001-10-19 2005-06-07 Prolico, Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
US20090239233A1 (en) * 2002-01-25 2009-09-24 Applera Corporation Single-tube, ready-to-use assay kits, and methods using same
US20040072195A1 (en) * 2002-01-25 2004-04-15 Hunkapiller Michael W. Single-tube, ready-to-use assay kits, and methods using same
CA2474482A1 (en) * 2002-01-25 2003-08-07 Applera Corporation Methods for placing, accepting, and filling orders for products and services
CA2472554A1 (en) * 2002-02-27 2003-09-04 Biosource International Inc. Methods of using fet labeled oligonucleotides that include a 3' 5' exonuclease resistant quencher domain and compositions for practicing the same
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
WO2003078450A2 (en) * 2002-03-11 2003-09-25 Epoch Biosciences, Inc. Negatively charged minor groove binders
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
US7109316B2 (en) * 2002-08-23 2006-09-19 Ce Healthcare Bio-Sciences Corp. Oligonucleotide tagged nucleoside triphosphates (OTNTPs) for genetic analysis
JP4694201B2 (ja) * 2002-09-20 2011-06-08 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド アントラキノン消光色素、それらの製造方法及び使用
EP1405920A3 (en) * 2002-10-02 2004-04-14 Roche Diagnostics GmbH Improved FRET process
CN100439515C (zh) * 2003-03-03 2008-12-03 清华大学 一种核酸分析芯片实验室系统与应用
CN1280428C (zh) * 2003-05-19 2006-10-18 清华大学 一种基于微小颗粒的生物芯片系统及其应用
US20070269803A1 (en) * 2003-06-26 2007-11-22 Khalil Arar Fluorogenic Nucleic Acid Probes Including Lna for Methods to Detect and/or Quantify Nucleic Acid Analytes
PT1687609E (pt) * 2003-10-28 2015-03-02 Epoch Biosciences Inc Sondas fluorescentes para a detecção de adn por hibridação com uma maior sensibilidade e menor ruído de fundo
WO2005047471A2 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. Improved fret efficiency methods
US7439341B2 (en) 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US7618778B2 (en) * 2004-06-02 2009-11-17 Kaufman Joseph C Producing, cataloging and classifying sequence tags
WO2006010038A2 (en) * 2004-07-08 2006-01-26 Biosearch Technologies, Inc. Inducible fluorescence assay
US7319022B1 (en) * 2004-08-13 2008-01-15 Epoch Biosciences, Inc. Amplification methods
AU2005277258B2 (en) 2004-08-19 2012-03-29 Blood Cell Storage, Inc Fluorescent pH detector system and related methods
US8497134B2 (en) * 2004-08-19 2013-07-30 Blood Cell Storage, Inc. Fluorescent detector systems for the detection of chemical perturbations in sterile storage devices
US8183052B2 (en) * 2004-08-19 2012-05-22 Blood Cell Storage, Inc. Methods and apparatus for sterility testing
EP1627926A1 (en) * 2004-08-19 2006-02-22 Roche Diagnostics GmbH A method to reduce false positive results
US8586718B2 (en) * 2004-09-14 2013-11-19 Applied Biosystems, Llc Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes
WO2006084067A2 (en) * 2005-02-04 2006-08-10 Berry & Associates, Inc. Dark quenchers, probes and other conjugates incorporating the same, their use
EP1866387B1 (en) * 2005-03-09 2013-05-08 Cepheid Polar dyes
KR101335218B1 (ko) 2005-05-02 2013-12-12 바스프 에스이 분석물의 감응성 검출을 위한 신규한 표지화 전략
TWI406952B (zh) * 2005-05-02 2013-09-01 Basf Ag 分析物之敏感偵測的新標記策略
WO2006122002A2 (en) * 2005-05-09 2006-11-16 Panomics, Inc. Multiplex capture of nucleic acids
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
WO2006124771A2 (en) * 2005-05-12 2006-11-23 Panomics, Inc. Multiplex branched-chain dna assays
JP2008545659A (ja) 2005-05-20 2008-12-18 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド オリゴヌクレオチドを標識する化合物及び方法
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
DK2500439T4 (da) * 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
DE102005029811B4 (de) * 2005-06-27 2009-03-12 Siemens Ag Oligonukleotidanordnungen, Verfahren zu deren Einsatz und deren Verwendung
WO2007044427A2 (en) * 2005-10-05 2007-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
WO2007059226A2 (en) * 2005-11-15 2007-05-24 The General Hospital Corporation Photoactivatable antimicrobial agents
US7345303B2 (en) * 2005-12-22 2008-03-18 Xerox Corporation Organic thin-film transistors
WO2007106907A2 (en) * 2006-03-15 2007-09-20 Integrated Dna Technologies, Inc. Nucleic acid monomers with 2'-chemical moieties
WO2008002920A2 (en) * 2006-06-26 2008-01-03 Epoch Biosciences, Inc. Methods for generating target nucleic acid sequences
JP5646172B2 (ja) 2006-10-31 2014-12-24 ベースクリック ゲーエムベーハー リポーター分子の製造のためのクリックケミストリー
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
US7960139B2 (en) 2007-03-23 2011-06-14 Academia Sinica Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells
EP2147009A4 (en) 2007-05-18 2014-01-08 Dharmacon Inc NOVEL CHROMOPHORE SILYL PROTECTION GROUPS AND THEIR USE IN THE CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES
US8084589B2 (en) * 2007-08-31 2011-12-27 University Of Massachusetts Phosphoramidite nucleoside analogs
JP4557014B2 (ja) * 2008-02-14 2010-10-06 ソニー株式会社 核酸検出用蛍光標識オリゴヌクレオチド、及び該核酸検出用蛍光標識オリゴヌクレオチドを用いた二本鎖形成に関わる情報を得る方法
EP2268608A4 (en) * 2008-04-01 2012-01-11 Biosearch Technologies Inc DARK NUCLEIC ACID STABILIZED FLUOROPHORE DEACTIVATOR PROBES
WO2009137062A2 (en) * 2008-05-05 2009-11-12 The General Hospital Corporation Photoactivatable antimicrobial agents and therapeutic and diagnostic methods of using same
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
ES2442024T3 (es) 2008-07-15 2014-02-07 Academia Sinica Matrices de glucano sobre portaobjetos de vidrio revestidos con aluminio de tipo PTFE y métodos relacionados
CN102171368B (zh) 2008-10-01 2017-06-20 皇家飞利浦电子股份有限公司 在载体上固定化核酸的方法
EP2334808B1 (en) * 2008-10-01 2017-11-15 Koninklijke Philips N.V. Method for testing and quality controlling of nucleic acids on a support
WO2010077324A2 (en) 2008-12-17 2010-07-08 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
EP3249053A1 (en) 2009-03-27 2017-11-29 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
CN104945509A (zh) 2009-09-16 2015-09-30 弗·哈夫曼-拉罗切有限公司 包含卷曲螺旋和/或系链的蛋白质复合体及其用途
US10087236B2 (en) 2009-12-02 2018-10-02 Academia Sinica Methods for modifying human antibodies by glycan engineering
US11377485B2 (en) 2009-12-02 2022-07-05 Academia Sinica Methods for modifying human antibodies by glycan engineering
WO2011087707A1 (en) 2009-12-22 2011-07-21 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
TW201138821A (en) 2010-03-26 2011-11-16 Roche Glycart Ag Bispecific antibodies
US8916345B2 (en) * 2010-03-26 2014-12-23 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
WO2011130332A1 (en) 2010-04-12 2011-10-20 Academia Sinica Glycan arrays for high throughput screening of viruses
EP3502272A1 (en) 2010-06-21 2019-06-26 Life Technologies Corporation Compositions, kits, and methods for synthesis and/or detection of nucleic acids
US9005895B2 (en) 2010-06-21 2015-04-14 Life Technologies Corporation Compositions, methods and kits for nucleic acid synthesis and amplification
CA2809457C (en) 2010-09-07 2019-07-30 Integrated Dna Technologies, Inc. Modifications for antisense compounds
ES2648564T3 (es) 2010-10-21 2018-01-04 Advanced Cell Diagnostics, Inc. Método ultrasensible para la detección in situ de ácidos nucleicos
KR20120042100A (ko) * 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
CN103384825B (zh) 2010-12-23 2017-05-24 霍夫曼-拉罗奇有限公司 通过二价结合剂检测经翻译后修饰的多肽
EP2655413B1 (en) 2010-12-23 2019-01-16 F.Hoffmann-La Roche Ag Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
US8969003B2 (en) 2011-03-23 2015-03-03 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
EP2689031B1 (en) 2011-03-23 2016-10-19 ELITechGroup B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
ES2537189T3 (es) 2011-05-24 2015-06-03 Elitech Holding B.V. Detección de estafilococos áureos resistentes a la meticilina
US9040307B2 (en) 2011-05-27 2015-05-26 Blood Cell Storage, Inc. Fluorescent pH detector system and related methods
CN103906848B (zh) 2011-08-18 2016-03-02 雀巢产品技术援助有限公司 用于检测等位变体的组合物和方法
BR112014019579A2 (pt) 2012-02-10 2019-10-15 Genentech, Inc Anticorpo de cadeia única, polinucleotídeo, vetor, célula hospedeira, método de produção de um anticorpo de cadeia única, heteromultímero e método de produção do heteromultímero
CA2867916C (en) 2012-03-22 2020-05-26 Lgc Genomics Limited Polymerase chain reaction detection system using oligonucleotides comprising a phosphorothioate group
US10130714B2 (en) 2012-04-14 2018-11-20 Academia Sinica Enhanced anti-influenza agents conjugated with anti-inflammatory activity
US20160073634A1 (en) 2012-06-22 2016-03-17 The General Hospital Corporation Beta-lactamase targeted photosensitizer for pesticide and pest detection
RU2639287C2 (ru) 2012-06-27 2017-12-20 Ф. Хоффманн-Ля Рош Аг Способ отбора и получения высокоселективных и мультиспецифичных нацеливающих групп с заданными свойствами, включающих по меньшей мере две различные связывающие группировки, и их применения
WO2014001325A1 (en) 2012-06-27 2014-01-03 F. Hoffmann-La Roche Ag Method for making antibody fc-region conjugates comprising at least one binding entity that specifically binds to a target and uses thereof
US9914956B2 (en) 2012-08-18 2018-03-13 Academia Sinica Cell-permeable probes for identification and imaging of sialidases
GB2515990B (en) 2013-03-06 2016-05-04 Lgc Genomics Ltd Polymerase chain reaction detection system
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
US20140255928A1 (en) 2013-03-11 2014-09-11 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
WO2014164874A2 (en) 2013-03-13 2014-10-09 Becton, Dickinson And Company Methods and compositions for modulation of amplification efficiency
EP2971086A1 (en) 2013-03-14 2016-01-20 ELITechGroup B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
EP2986597A4 (en) * 2013-04-19 2016-11-16 Agency Science Tech & Res ADJUSTABLE FLUORESCENCE USING CLIENTS
ES2654631T3 (es) 2013-05-13 2018-02-14 Elitechgroup B.V. PCR digital en gotas con sondas cortas de ranura menor
EP3013365B1 (en) 2013-06-26 2019-06-05 Academia Sinica Rm2 antigens and use thereof
WO2014210564A1 (en) 2013-06-27 2014-12-31 Academia Sinica Glycan conjugates and use thereof
CA2923579C (en) 2013-09-06 2023-09-05 Academia Sinica Human inkt cell activation using glycolipids with altered glycosyl groups
US10150818B2 (en) 2014-01-16 2018-12-11 Academia Sinica Compositions and methods for treatment and detection of cancers
AU2015206370A1 (en) 2014-01-16 2016-07-07 Academia Sinica Compositions and methods for treatment and detection of cancers
CN106415244B (zh) 2014-03-27 2020-04-24 中央研究院 反应性标记化合物及其用途
EP2942401A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Detection of DNA that originates from a specific cell-type
EP2942400A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
EP3140421B8 (en) 2014-05-09 2020-05-06 Eurofins LifeCodexx GmbH Detection of dna that originates from a specific cell-type and related methods
US10118969B2 (en) 2014-05-27 2018-11-06 Academia Sinica Compositions and methods relating to universal glycoforms for enhanced antibody efficacy
EP3149037A4 (en) 2014-05-27 2018-01-10 Academia Sinica Anti-her2 glycoantibodies and uses thereof
KR20230033737A (ko) 2014-05-27 2023-03-08 아카데미아 시니카 증진된 항체 효능을 위한 범용 당형태에 관한 조성물 및 방법
KR20220151036A (ko) 2014-05-27 2022-11-11 아카데미아 시니카 항-ｃｄ20 글리코항체 및 이의 용도
KR102494193B1 (ko) 2014-05-28 2023-01-31 아카데미아 시니카 항-ｔｎｆ-알파 글리코항체 및 이의 용도
WO2016040369A2 (en) 2014-09-08 2016-03-17 Academia Sinica HUMAN iNKT CELL ACTIVATION USING GLYCOLIPIDS
ES2764111T3 (es) 2014-12-03 2020-06-02 Hoffmann La Roche Anticuerpos multiespecíficos
US10266903B2 (en) 2014-12-12 2019-04-23 Elitechgroup, Inc. Methods and compositions for detecting antibiotic resistant bacteria
EP3230468B1 (en) 2014-12-12 2020-09-16 ELITechGroup, Inc. Methods and kits for detecting antibiotic resistant bacteria
US10495645B2 (en) 2015-01-16 2019-12-03 Academia Sinica Cancer markers and methods of use thereof
US9975965B2 (en) 2015-01-16 2018-05-22 Academia Sinica Compositions and methods for treatment and detection of cancers
BR112017013027A2 (pt) * 2015-01-20 2018-01-09 Biosearch Tech Inc compostos à base de cumarina e métodos relacionados
CA2972072A1 (en) 2015-01-24 2016-07-28 Academia Sinica Novel glycan conjugates and methods of use thereof
US9957393B2 (en) * 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
US11078528B2 (en) 2015-10-12 2021-08-03 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
HUE050491T2 (hu) 2015-11-10 2020-12-28 Eurofins Lifecodexx Gmbh Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között
CN108463560B (zh) 2016-01-15 2022-09-20 昆塔波尔公司 具有降低的背景的基于光学的纳米孔分析
JP2019515876A (ja) 2016-03-08 2019-06-13 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ Ｎ−グリカンおよびそのアレイのモジュール合成のための方法
EP3440221A1 (en) 2016-04-06 2019-02-13 Life Technologies Corporation Compositions, methods, and kits for synthesis and detection of nucleic acids
WO2017200790A1 (en) 2016-05-20 2017-11-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Real-time reverse transcriptase-polymerase chain reaction assay with modified probe for the diagnosis of rabies viruses and other lyssaviruses
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
US10781175B2 (en) 2016-07-15 2020-09-22 Am Chemicals Llc Solid supports and phosphoramidite building blocks for oligonucleotide conjugates
CA3031812A1 (en) 2016-08-19 2018-02-22 Quantapore, Inc. Optically-based nanopore sequencing using quenching agents
JP7213549B2 (ja) 2016-08-22 2023-01-27 シーエイチオー ファーマ インコーポレイテッド 抗体、結合性断片、および使用の方法
US10415080B2 (en) 2016-11-21 2019-09-17 Nanostring Technologies, Inc. Chemical compositions and methods of using same
WO2018162986A2 (en) 2017-03-09 2018-09-13 Elitechgroup B.V. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
EP3668884B1 (en) * 2017-08-17 2022-03-09 ELITechGroup Inc. Duplex stabilizing fluorescence quenchers for nucleic acid probes
GB201713251D0 (en) 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
ES2967690T3 (es) 2018-05-07 2024-05-03 Cepheid Colorantes de fosforamidita de sulforodamina
AU2019271028A1 (en) 2018-05-14 2020-12-03 Nanostring Technologies, Inc. Chemical compositions and methods of using same
EP3802526A1 (en) 2018-05-29 2021-04-14 ELITechGroup, Inc. Carborhodamine compounds and methods of preparation thereof
EP3830301A1 (en) 2018-08-01 2021-06-09 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
WO2020037290A1 (en) 2018-08-16 2020-02-20 Life Technologies Corporation Reagents, mixtures, kits and methods for amplification of nucleic acids
WO2020142754A2 (en) 2019-01-04 2020-07-09 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
CN113811607A (zh) 2019-03-07 2021-12-17 加利福尼亚大学董事会 CRISPR-Cas效应子多肽和其使用方法
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2021240443A1 (en) 2020-05-27 2021-12-02 King Abdullah University Of Science And Technology Iscan: an rt-lamp-coupled crispr-cas module for rapid, sensitive detection of sars-cov-2
CA3185976A1 (en) 2020-07-16 2022-01-20 Kevin P. Lund Fluorescent dyes
CA3186955A1 (en) 2020-07-23 2022-01-27 Scott Benson Energy transfer dye conjugates for use in biological assays
EP4185858A2 (en) 2020-07-23 2023-05-31 Life Technologies Corporation Compositions, systems and methods for biological analysis using dyes
WO2023004400A1 (en) 2021-07-21 2023-01-26 Life Technologies Corporation Dibenzoxanthene quenchers, uses, and methods of preparation
WO2023023673A2 (en) 2021-08-20 2023-02-23 Kasa Bio, L.L.C. Compositions and methods for multiplex detection of mirna and other polynucelotides

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US952202A (en) * 1909-05-22 1910-03-15 Heinrich Klingenfeld Combined garment support and form.
US2264303A (en) 1940-03-27 1941-12-02 Eastman Kodak Co Azo compounds and material colored therewith
US3407189A (en) 1956-07-25 1968-10-22 Sandoz Ltd Water-insoluble monoazo dyestuffs
GB1394368A (en) 1972-12-14 1975-05-14 Ici Ltd Disperse azo dyestuffs
US3970617A (en) * 1974-03-07 1976-07-20 E. I. Du Pont De Nemours And Company Navy-blue nitrophenylazonaphthylamino dyes
GB1533121A (en) 1975-08-04 1978-11-22 Ici Ltd Disperse nitro disazo dyestuffs
US4358535A (en) 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US4570542A (en) * 1982-05-17 1986-02-18 Weld Tooling Corporation Ribbon rail systems
DE3526565A1 (de) 1985-07-25 1987-02-05 Boehringer Mannheim Gmbh Resorufin-derivate, verfahren zu deren herstellung sowie deren verwendung in fluoreszenzimmunoassays
FR2588495B1 (fr) * 1985-10-16 1987-12-11 Aerospatiale Ensemble de douille de fixation pour unite d'usinage et unite d'usinage adaptee pour cooperer avec ladite douille
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5824796A (en) 1988-09-28 1998-10-20 Epoch Pharmaceuticals, Inc. Cross-linking oligonucleotides
US4850763A (en) * 1988-10-03 1989-07-25 The Boeing Company Tool track for use on aircraft
US5272259A (en) * 1988-12-23 1993-12-21 Bayer Aktiengesellschaft Stilbene dyestuffs and light-polarizing films or sheets containing stilbene
EP0472648A4 (en) 1989-05-18 1992-09-16 Microprobe Corporation Crosslinking oligonucleotides
DE3916595A1 (de) 1989-05-22 1990-11-29 Boehringer Mannheim Gmbh Verfahren zur nichtradioaktiven messung der nucleinsaeuresynthese in eukaryontischen zellen
US5451463A (en) 1989-08-28 1995-09-19 Clontech Laboratories, Inc. Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5512667A (en) 1990-08-28 1996-04-30 Reed; Michael W. Trifunctional intermediates for preparing 3'-tailed oligonucleotides
WO1992010588A1 (en) 1990-12-06 1992-06-25 Affymax Technologies N.V. Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides
JPH04279556A (ja) * 1991-03-07 1992-10-05 Nippon Oil & Fats Co Ltd フルオロアルキル基含有アゾ化合物及びその製造方法
EP0528512A1 (en) * 1991-08-20 1993-02-24 Pioneer Electronic Corporation Recording medium
JPH0578300A (ja) * 1991-09-19 1993-03-30 Nippon Oil & Fats Co Ltd フルオロアルキル基含有アゾ化合物及びその製造方法
CA2123133C (en) 1991-11-07 2005-01-04 Michael J. Heller Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
TW393513B (en) 1991-11-26 2000-06-11 Isis Pharmaceuticals Inc Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5502177A (en) * 1993-09-17 1996-03-26 Gilead Sciences, Inc. Pyrimidine derivatives for labeled binding partners
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5395187A (en) * 1993-11-22 1995-03-07 Mcdonnell Douglas Corporation Method and apparatus for attaching a drill motor to a drill plate with a clamping device having an expandable collet
JPH07179773A (ja) * 1993-12-01 1995-07-18 Dainichiseika Color & Chem Mfg Co Ltd アゾ化合物
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5482411A (en) * 1994-12-22 1996-01-09 Mcdonnell Douglas Corporation Method and apparatus for securely clamping a drill motor to a drill plate
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US20010018514A1 (en) 1998-07-31 2001-08-30 Mcgall Glenn H. Nucleic acid labeling compounds
GB9602265D0 (en) 1996-02-05 1996-04-03 Amersham Int Plc Benzophenoxazine dyes
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
US6036409A (en) * 1997-01-23 2000-03-14 The Boeing Company Blind hole self-collet countersink
US5846726A (en) 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
AU9220498A (en) 1997-09-04 1999-03-22 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6007992A (en) 1997-11-10 1999-12-28 Gilead Sciences, Inc. Pyrimidine derivatives for labeled binding partners
US6028183A (en) 1997-11-07 2000-02-22 Gilead Sciences, Inc. Pyrimidine derivatives and oligonucleotides containing same
US6210084B1 (en) * 1997-11-26 2001-04-03 The Boeing Company Pressure foot assembly for clamping a joint
CA2318880C (en) 1998-02-04 2010-07-27 Perkin-Elmer Corporation Determination of a genotype of an amplification product at multiple allelic sites
US6220099B1 (en) * 1998-02-17 2001-04-24 Ce Nuclear Power Llc Apparatus and method for performing non-destructive inspections of large area aircraft structures
US6073328A (en) * 1998-02-28 2000-06-13 Hendricks; James Carpet installation tool
US5952202A (en) 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
US6117986A (en) * 1998-06-10 2000-09-12 Intergen Company, L.P. Pyrimidines linked to a quencher
US6240322B1 (en) * 1998-11-04 2001-05-29 Cardiac Pacemakers, Inc. System and apparatus having low profile collapsible tines
US6399392B1 (en) 1999-04-23 2002-06-04 Molecular Probes, Inc. Xanthene dyes and their application as luminescence quenching compounds
US6151241A (en) 1999-05-19 2000-11-21 Symetrix Corporation Ferroelectric memory with disturb protection
US6114518A (en) 1999-09-30 2000-09-05 Becton, Dickinson And Company Synthesis and use of labelled phosphoramidite compositions
US6467385B1 (en) * 1999-12-03 2002-10-22 The Boeing Company Panel trimming system
US6727356B1 (en) * 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US6465644B1 (en) 2000-05-02 2002-10-15 Applera Corporation Sulfonated [8,9] benzophenoxazine dyes and the use of their labelled conjugates
US6430796B1 (en) * 2000-05-03 2002-08-13 The Boeing Company Apparatus for performing automated manufacturing operations on panel-shaped workpieces
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US6550129B1 (en) * 2000-09-25 2003-04-22 The Boeing Company Portable computer numeric controlled manufacturing machines and associated methods
US6448407B1 (en) 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis

Also Published As

Publication number Publication date
AU2074801A (en) 2001-06-18
AU782204B2 (en) 2005-07-14
US20020034754A1 (en) 2002-03-21
US6653473B2 (en) 2003-11-25
US6727356B1 (en) 2004-04-27
ES2381861T3 (es) 2012-06-01
JP2003516163A (ja) 2003-05-13
EP1235938B1 (en) 2012-02-08
US20030008304A1 (en) 2003-01-09
US6790945B2 (en) 2004-09-14
WO2001042505A3 (en) 2002-01-24
US6699975B2 (en) 2004-03-02
US20020155484A1 (en) 2002-10-24
JP5019688B2 (ja) 2012-09-05
US20040191796A1 (en) 2004-09-30
JP2012175975A (ja) 2012-09-13
EP1235938A2 (en) 2002-09-04
CA2396795A1 (en) 2001-06-14
WO2001042505A2 (en) 2001-06-14

Similar Documents

Publication Publication Date Title
ATE544771T1 (de) Fluoreszent quenching detektion reagenzien und verfahren
Im Jun et al. Rotaxane-based molecular switch with fluorescence signaling
Han et al. Recent advances in chromo-fluorogenic probes for fluoride detection
WO1999064431A3 (en) Purines and pyrimidines linked to a quencher
ES2164417T3 (es) Cromoforo fluorescente, enlazado de forma covalente a un material de soporte organico.
WO2004085539A3 (en) Cyanine dye labelling reagents with meso-substitution
WO2005049849A3 (en) Fluorescence quenching azo dyes, their methods of preparation and use
CA2222275A1 (en) Fluorinated xanthene derivatives
Kam et al. Tronocarpine, a novel pentacyclic indole incorporating a seven-membered lactam moiety
CA2139348A1 (en) Improved chemiluminescent 1,2-dioxetanes
CA2152446A1 (en) Covalent Cyanine Dye Oligonucleotide Conjugates
ECSP055975A (es) Tinturas de cianina hidrofílicas, reactivas con tiol y conjugados de las mismas con biomoléculas para diagnóstico por fluorescencia
ATE212367T1 (de) Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher
WO2003019145A3 (en) Non-fluorescent quencher compounds and biomolecular assays
WO2003002974A3 (en) Ecl labels having improved non-specific binding properties, methods of using and kits containing the same
WO2000023446A8 (de) Kernerweiterte perylenbisimide und ihre verwendung als fluoreszenzfarbstoffe
WO2006044275A3 (en) Methods for altering surface characteristics of microspheres
DE69431856D1 (de) 9-Amino und 9-Nitrocamptothecinderivate, Verfahren zu ihrer Herstellung unf ihre Verwendung als Antitumormittel
CA2299080A1 (en) Fluorescent tracer dyes
ES2108109T3 (es) Nuevos oligonucleotidos conjugados con quelatos de lantanidos.
Chen et al. A ratiometric fluorescent sensor for zinc (II) with high selectivity
Uchida et al. Effects of methyl substitution on electronic relaxation processes of pyrimidine vapor
CA2427831A1 (en) Cyclization process step in the making of quinolones and naphthyridines
CA2435280A1 (en) Chemiluminescent solution based on disubstituted perylene tetracarboxylic acids, their dianhydrides and diimides
WO2006044413A3 (en) Methods for forming dyed microspheres and populations of dyed microspheres