ATE445704T1 - Expressionsvektor für tierische zellen - Google Patents
Expressionsvektor für tierische zellenInfo
- Publication number
- ATE445704T1 ATE445704T1 AT01957013T AT01957013T ATE445704T1 AT E445704 T1 ATE445704 T1 AT E445704T1 AT 01957013 T AT01957013 T AT 01957013T AT 01957013 T AT01957013 T AT 01957013T AT E445704 T1 ATE445704 T1 AT E445704T1
- Authority
- AT
- Austria
- Prior art keywords
- vector
- expression vector
- present
- animal cells
- expression
- Prior art date
Links
- 239000013604 expression vector Substances 0.000 title abstract 4
- 241001465754 Metazoa Species 0.000 title abstract 2
- 239000013598 vector Substances 0.000 abstract 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 2
- 108010052343 Gastrins Proteins 0.000 abstract 1
- 101000899111 Homo sapiens Hemoglobin subunit beta Proteins 0.000 abstract 1
- 210000004102 animal cell Anatomy 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000005030 transcription termination Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4717—Plasma globulins, lactoglobulin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/595—Gastrins; Cholecystokinins [CCK]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Endocrinology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2000-0043996A KR100408844B1 (ko) | 2000-07-29 | 2000-07-29 | 동물세포 발현벡터 |
| PCT/KR2001/001285 WO2002014525A2 (en) | 2000-07-29 | 2001-07-27 | Expression vector using for animal cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE445704T1 true ATE445704T1 (de) | 2009-10-15 |
Family
ID=19680759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01957013T ATE445704T1 (de) | 2000-07-29 | 2001-07-27 | Expressionsvektor für tierische zellen |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7422874B2 (de) |
| EP (1) | EP1392835B1 (de) |
| JP (1) | JP4138479B2 (de) |
| KR (1) | KR100408844B1 (de) |
| CN (1) | CN1262663C (de) |
| AT (1) | ATE445704T1 (de) |
| AU (1) | AU2001278797A1 (de) |
| DE (1) | DE60140218D1 (de) |
| ES (1) | ES2333774T3 (de) |
| WO (1) | WO2002014525A2 (de) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2435972C (en) * | 2001-01-26 | 2011-09-13 | University Of Lausanne | Matrix attachment regions and methods for use thereof |
| CA2484000A1 (en) | 2002-05-24 | 2003-12-04 | Schering Corporation | Neutralizing human anti-igfr antibody |
| AU2003263161A1 (en) * | 2002-09-20 | 2004-04-08 | Novo Nordisk A/S | Method for making recombinant proteins |
| US6933136B2 (en) | 2002-09-20 | 2005-08-23 | Novo Nordisk A/S | Method for making recombinant proteins |
| US7135338B2 (en) * | 2003-06-11 | 2006-11-14 | Dobeel Corporation | Methods for overexpression of high molecular weight form of mannose binding lectin (MBL) and a specific formulation for active treatment for systemic infection with microorganism |
| KR100755931B1 (ko) * | 2003-06-11 | 2007-09-06 | (주)두비엘 | 고분자형 만노스 결합형 렉틴의 대량 생산방법 및 상기방법으로 제조된 만노스 결합형 렉틴 재조합 단백질의제제화 |
| CN1863913B (zh) | 2003-10-24 | 2011-01-12 | 思兰克斯有限公司 | 通过mar序列的多次转染过程在哺乳动物细胞中实现高效基因转移和表达 |
| JP2007510434A (ja) * | 2003-11-12 | 2007-04-26 | シェーリング コーポレイション | 多重遺伝子発現のためのプラスミドシステム |
| PE20050928A1 (es) | 2003-11-21 | 2005-11-08 | Schering Corp | Combinaciones terapeuticas de anticuerpo anti-igfr1 |
| WO2006046786A1 (en) * | 2004-10-28 | 2006-05-04 | Dobeel Co., Ltd. | Method for the mass production of multimeric mannose binding lectin |
| KR20060096383A (ko) * | 2005-03-04 | 2006-09-11 | 주식회사 셀트리온 | 1 카피 이상의 mar dna 서열이 유전자의전사종결영역의 3′말단에 삽입된 동물세포 발현 벡터 및그를 이용한 외래 유전자의 발현 방법 |
| KR100779761B1 (ko) * | 2005-10-28 | 2007-11-30 | 대한민국 | 인슐린 억제 및 사람 ide 유전자로 형질전환된 동물 및그 제조방법 |
| KR100836745B1 (ko) * | 2007-01-31 | 2008-06-10 | (주)두비엘 | Hbv 백신 및 그의 제조 방법 |
| EP2164971B1 (de) | 2007-06-15 | 2015-12-09 | Mogam Biotechnology Research Institute | Verfahren zur herstellung von aktivem rekombinantem blutgerinnungsfaktor ix |
| CN101348786B (zh) * | 2007-07-20 | 2011-05-11 | 谭孟群 | 一种人β珠蛋白基因及其重组腺相关病毒载体 |
| WO2009150456A1 (en) * | 2008-06-10 | 2009-12-17 | Isis Innovation Limited | Protein expression |
| KR101054362B1 (ko) | 2008-07-03 | 2011-08-05 | 재단법인 목암생명공학연구소 | 재조합 단백질의 푸코스 함량을 감소시키는 방법 |
| US8137933B2 (en) | 2008-11-12 | 2012-03-20 | Schering Corporation | Mammalian expression vector pUHAB |
| JP5688771B2 (ja) * | 2009-03-27 | 2015-03-25 | 国立大学法人広島大学 | 哺乳動物細胞内で目的遺伝子を増幅し高発現させる方法、および当該方法を実施するために用いられるキット |
| CN102575264A (zh) * | 2009-09-18 | 2012-07-11 | 瑟莱克斯公司 | 增加的转基因表达和加工的产品和方法 |
| US20130034875A1 (en) * | 2009-10-01 | 2013-02-07 | Toto Ltd. | Dna construct, and process for production of recombinant cho cell using same |
| EP2484761A4 (de) * | 2009-10-01 | 2013-11-27 | Toto Ltd | Dna-konstrukt und verfahren zur herstellung rekombinanter cho-zellen damit |
| NZ609903A (en) | 2010-10-08 | 2015-04-24 | Cadila Healthcare Ltd | Expression vector for high level expression of recombinant proteins |
| WO2012134215A2 (ko) * | 2011-03-30 | 2012-10-04 | (주)팬젠 | 동물세포 발현벡터 |
| WO2012133695A1 (ja) | 2011-03-31 | 2012-10-04 | クニミネ工業株式会社 | タンパク質結晶化条件探索剤及びタンパク質結晶化条件探索方法 |
| KR101420274B1 (ko) * | 2011-06-13 | 2014-07-21 | 주식회사 종근당 | Csp-b 5'sar 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법 |
| CA2849318C (en) | 2011-09-22 | 2019-11-12 | Amgen Inc. | Cd27l antigen binding proteins |
| MX355624B (es) | 2011-12-22 | 2018-04-25 | Hoffmann La Roche | Combinaciones de elementos de vector de expresion, metodos novedosos de generacion de celulas de produccion y su uso para la produccion recombinante de polipeptidos. |
| SG10201805622QA (en) | 2011-12-22 | 2018-08-30 | Hoffmann La Roche | Expression Vector Organization, Novel Production Cell Generation Methods And Their Use For The Recombinant Production Of Polypeptides |
| UY34813A (es) | 2012-05-18 | 2013-11-29 | Amgen Inc | Proteínas de unión a antígeno dirigidas contra el receptor st2 |
| UY35148A (es) | 2012-11-21 | 2014-05-30 | Amgen Inc | Immunoglobulinas heterodiméricas |
| US9546203B2 (en) | 2013-03-14 | 2017-01-17 | Amgen Inc. | Aglycosylated Fc-containing polypeptides with cysteine substitutions |
| WO2014144817A2 (en) | 2013-03-15 | 2014-09-18 | Amgen Inc. | Inhibitory polypeptides specific to wnt inhibitors |
| CN103194455B (zh) * | 2013-04-08 | 2014-10-15 | 山东省农业科学院畜牧兽医研究所 | 高效表达的猪粒细胞巨噬细胞集落刺激因子基因及其表达蛋白的用途 |
| ES2895824T3 (es) | 2013-05-30 | 2022-02-22 | Kiniksa Pharmaceuticals Ltd | Proteínas de enlace al antígeno del receptor de oncastatina M |
| CA2922838A1 (en) * | 2013-09-05 | 2015-03-12 | Sarepta Therapeutics, Inc. | Antisense-induced exon2 inclusion in acid alpha-glucosidase |
| JP6668241B2 (ja) | 2013-09-05 | 2020-03-18 | アムジエン・インコーポレーテツド | 予測可能で、一貫性のある、且つ再現可能な糖型特性を示すFc含有分子 |
| KR101591823B1 (ko) | 2013-12-27 | 2016-02-04 | 재단법인 목암생명공학연구소 | 증가된 유전자 발현능을 갖는 발현벡터 |
| TN2017000432A1 (en) | 2015-04-10 | 2019-04-12 | Amgen Inc | Interleukin-2 muteins for the expansion of t-regulatory cells |
| CN116063543A (zh) | 2015-04-24 | 2023-05-05 | 豪夫迈·罗氏有限公司 | 多特异性抗原结合蛋白 |
| AU2016329001A1 (en) | 2015-09-22 | 2018-03-29 | Genentech, Inc. | Expression of Fc-containing proteins |
| WO2017059551A1 (en) | 2015-10-08 | 2017-04-13 | Zymeworks Inc. | Antigen-binding polypeptide constructs comprising kappa and lambda light chains and uses thereof |
| BR112018007356A2 (pt) | 2015-11-02 | 2018-10-23 | Genentech Inc | métodos de produção de formas fucosiladas e não fucosiladas de uma proteína |
| MX2018012695A (es) | 2016-04-18 | 2019-06-20 | Sarepta Therapeutics Inc | Oligomeros antisentido y metodos de uso de los mismos para tratar enfermedades relacionadas con el gen alpha-glucosidasa acido. |
| EP3280725B1 (de) | 2016-05-04 | 2020-08-26 | Amgen Inc. | Interleukin-2-muteine zur expansion von t-regulatorischen zellen |
| WO2018150345A1 (en) * | 2017-02-16 | 2018-08-23 | De Vita Bio Life Sciences | An expression vector |
| KR102611853B1 (ko) | 2017-06-30 | 2023-12-08 | 자임워크스 비씨 인코포레이티드 | 안정화된 키메라 fabs |
| CN110343718A (zh) * | 2018-04-03 | 2019-10-18 | 新乡医学院 | 一种高效稳定的细胞表达载体、表达系统及其制备方法、应用 |
| JP2022544236A (ja) | 2019-08-13 | 2022-10-17 | アムジエン・インコーポレーテツド | 制御性t細胞の増殖のためのインターロイキン-2変異タンパク質 |
| JOP20220151A1 (ar) | 2019-12-17 | 2023-01-30 | Amgen Inc | ناهض مستقبل إنترلوكين -2 / tnf المزدوج للاستخدام في العلاج |
| WO2025006529A1 (en) | 2023-06-27 | 2025-01-02 | Amgen Inc. | Charge pair mutations to enable correct heavy-light chain pairing |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4956288A (en) * | 1988-04-22 | 1990-09-11 | Biogen, Inc. | Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA |
| US5108901A (en) * | 1988-09-02 | 1992-04-28 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
| ES2176208T3 (es) | 1992-10-05 | 2002-12-01 | Univ North Carolina State | Metodo para incrementar la expresion y para reducir la variabilidad de expresion de genes foraneos en celulas vegetales. |
| US5738985A (en) * | 1993-04-02 | 1998-04-14 | Ribogene, Inc. | Method for selective inactivation of viral replication |
| US5773695A (en) | 1996-01-26 | 1998-06-30 | North Carolina State University | Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells |
| CA2256484A1 (en) | 1996-06-06 | 1997-12-11 | Novartis Ag | Vectors comprising sar elements |
| EP0914454B1 (de) | 1996-06-11 | 2005-03-09 | Pioneer Hi-Bred International, Inc. | Ein synthetischer, pflanzlicher kernpromotor und stromaufwaerts gelegenes, regulatorisches element |
| US6245974B1 (en) | 1997-08-06 | 2001-06-12 | North Carolina State University | Matrix attachment regions |
| KR100313559B1 (ko) * | 1998-07-22 | 2002-03-08 | 허영섭 | 수용성 TGFβ 수용체를 발현하는 재조합 세포주 및 전기세포주를 이용한 암치료 방법 |
| US6388066B1 (en) * | 1998-09-29 | 2002-05-14 | Pioneer Hi-Bred International, Inc. | MAR/SAR elements flanking RSYN7-driven construct |
| CA2435972C (en) * | 2001-01-26 | 2011-09-13 | University Of Lausanne | Matrix attachment regions and methods for use thereof |
-
2000
- 2000-07-29 KR KR10-2000-0043996A patent/KR100408844B1/ko not_active Expired - Lifetime
-
2001
- 2001-07-27 EP EP01957013A patent/EP1392835B1/de not_active Expired - Lifetime
- 2001-07-27 AT AT01957013T patent/ATE445704T1/de not_active IP Right Cessation
- 2001-07-27 AU AU2001278797A patent/AU2001278797A1/en not_active Abandoned
- 2001-07-27 CN CNB018135579A patent/CN1262663C/zh not_active Expired - Lifetime
- 2001-07-27 ES ES01957013T patent/ES2333774T3/es not_active Expired - Lifetime
- 2001-07-27 JP JP2002519651A patent/JP4138479B2/ja not_active Expired - Lifetime
- 2001-07-27 DE DE60140218T patent/DE60140218D1/de not_active Expired - Lifetime
- 2001-07-27 WO PCT/KR2001/001285 patent/WO2002014525A2/en not_active Ceased
- 2001-07-27 US US10/343,303 patent/US7422874B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP4138479B2 (ja) | 2008-08-27 |
| EP1392835B1 (de) | 2009-10-14 |
| AU2001278797A1 (en) | 2002-02-25 |
| KR20020010327A (ko) | 2002-02-04 |
| US20040038394A1 (en) | 2004-02-26 |
| KR100408844B1 (ko) | 2003-12-06 |
| JP2004506428A (ja) | 2004-03-04 |
| WO2002014525A3 (en) | 2003-11-20 |
| US7422874B2 (en) | 2008-09-09 |
| CN1444657A (zh) | 2003-09-24 |
| ES2333774T3 (es) | 2010-03-01 |
| EP1392835A2 (de) | 2004-03-03 |
| EP1392835A4 (de) | 2006-04-19 |
| CN1262663C (zh) | 2006-07-05 |
| WO2002014525A2 (en) | 2002-02-21 |
| DE60140218D1 (de) | 2009-11-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |