BR112013008459A2 - ''vetor de expressão,célula hospedeira,processo para a produção de proteinas e de peptideos e dos variantes dos mesmos'' - Google Patents

''vetor de expressão,célula hospedeira,processo para a produção de proteinas e de peptideos e dos variantes dos mesmos''

Info

Publication number
BR112013008459A2
BR112013008459A2 BR112013008459A BR112013008459A BR112013008459A2 BR 112013008459 A2 BR112013008459 A2 BR 112013008459A2 BR 112013008459 A BR112013008459 A BR 112013008459A BR 112013008459 A BR112013008459 A BR 112013008459A BR 112013008459 A2 BR112013008459 A2 BR 112013008459A2
Authority
BR
Brazil
Prior art keywords
variants
protein
host cell
expression vector
peptide production
Prior art date
Application number
BR112013008459A
Other languages
English (en)
Inventor
Aashini Parikh
Ajit K Gupta
Arun Singh
Mansi Jakhade
Original Assignee
Cadila Healthcare Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cadila Healthcare Ltd filed Critical Cadila Healthcare Ltd
Publication of BR112013008459A2 publication Critical patent/BR112013008459A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR112013008459A 2010-10-08 2011-10-10 ''vetor de expressão,célula hospedeira,processo para a produção de proteinas e de peptideos e dos variantes dos mesmos'' BR112013008459A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2806MU2010 2010-10-08
PCT/IN2011/000703 WO2012046255A2 (en) 2010-10-08 2011-10-10 Expression vector for high level expression of recombinant proteins

Publications (1)

Publication Number Publication Date
BR112013008459A2 true BR112013008459A2 (pt) 2016-06-28

Family

ID=45464050

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013008459A BR112013008459A2 (pt) 2010-10-08 2011-10-10 ''vetor de expressão,célula hospedeira,processo para a produção de proteinas e de peptideos e dos variantes dos mesmos''

Country Status (12)

Country Link
US (1) US20130244280A1 (pt)
EP (1) EP2625277B1 (pt)
JP (1) JP5888616B2 (pt)
KR (1) KR101607734B1 (pt)
AR (1) AR083376A1 (pt)
AU (1) AU2011311189B2 (pt)
BR (1) BR112013008459A2 (pt)
CA (1) CA2813903A1 (pt)
EA (1) EA201390461A1 (pt)
NZ (1) NZ609903A (pt)
WO (1) WO2012046255A2 (pt)
ZA (1) ZA201302939B (pt)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112013025099B1 (pt) * 2011-03-30 2021-08-10 Pangen Biotech Inc. Vetor de expressão para células animais
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
KR20140015999A (ko) * 2012-07-27 2014-02-07 한화케미칼 주식회사 신규 ＭＡＲｓ 및 이를 이용하여 목적 단백질을 생산하는 방법
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
AU2013381687A1 (en) * 2013-03-12 2015-09-24 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
KR101591823B1 (ko) * 2013-12-27 2016-02-04 재단법인 목암생명공학연구소 증가된 유전자 발현능을 갖는 발현벡터
KR101599138B1 (ko) * 2014-06-17 2016-03-03 한국생명공학연구원 재조합 단백질 발현 증진을 위한 유전자 절편을 포함하는 벡터 및 이의 용도
CN105274112B (zh) * 2015-11-20 2018-01-16 江南大学 一种在酸性条件下诱导表达的启动子
CN107177611B (zh) * 2017-05-23 2020-03-20 江苏康禾生物制药有限公司 编码组织型纤溶酶原激活剂的dna分子及其重组细胞株
CN107502594A (zh) * 2017-07-14 2017-12-22 中国药科大学 一种稳定表达外源性ex－4基因的骨髓源间充质干细胞株
ES2821655T5 (es) * 2017-09-19 2024-08-06 Deutsches Krebsforsch Vectores de ADN no integrantes para la modificación genética de células
CN113025651B (zh) * 2021-03-31 2023-03-24 重庆医科大学 靶向HBV核心启动子的药物筛选细胞模型、Triciribine及结构类似物新应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731178A (en) 1990-03-21 1998-03-24 Behringwerke Aktiengesellschaft Attachment-elements for stimulation of eukaryotic expression systems
US5888774A (en) 1994-12-19 1999-03-30 Cangene Corporation Recombinant DNA molecules and expression vectors for erythropoietin
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
US7129062B2 (en) 2001-01-26 2006-10-31 Selexis Sa Matrix attachment regions and methods for use thereof
WO2005005644A1 (en) 2003-07-11 2005-01-20 Cytos Biotechnology Ag Gene expression system
US8772021B2 (en) 2005-03-04 2014-07-08 Celltrion, Inc. Expression vector for animal cell comprising at least one copy of MAR DNA sequences at the 3′ terminal of transcription termination region of a gene and method for the expression of foreign gene using the vector
EA011619B1 (ru) * 2005-06-20 2009-04-28 Кадила Хелзкэр Лимитед Экспрессионный вектор и способ продуцирования высоких уровней белков
EP2109674A2 (en) * 2007-01-08 2009-10-21 Millipore Corporation High expression cell line that eliminates gene amplification
CN101802170A (zh) * 2007-01-08 2010-08-11 米利波尔公司 在存在降低水平的一或多种污染物的条件下制备重组蛋白的细胞培养方法
WO2011015917A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant tnk-tpa (tenecteplase)

Also Published As

Publication number Publication date
CA2813903A1 (en) 2012-04-12
WO2012046255A8 (en) 2013-05-10
EP2625277B1 (en) 2017-09-06
KR20130094830A (ko) 2013-08-26
NZ609903A (en) 2015-04-24
AU2011311189A1 (en) 2013-05-30
JP5888616B2 (ja) 2016-03-22
AU2011311189B2 (en) 2015-10-08
KR101607734B1 (ko) 2016-03-30
EP2625277A2 (en) 2013-08-14
ZA201302939B (en) 2013-12-23
EA201390461A1 (ru) 2013-09-30
JP2013541953A (ja) 2013-11-21
WO2012046255A3 (en) 2012-05-31
AR083376A1 (es) 2013-02-21
US20130244280A1 (en) 2013-09-19
WO2012046255A2 (en) 2012-04-12

Similar Documents

Publication Publication Date Title
BR112013008459A2 (pt) ''vetor de expressão,célula hospedeira,processo para a produção de proteinas e de peptideos e dos variantes dos mesmos''
BR112012022046A2 (pt) ''anticorpo,composição farmacêutica,ácido nucleico ,vetores de expressão,célula hospedeira e método para a produção de um anticorpo recombinante''.
BR112013033350A2 (pt) imunocitocina, ácido nucleico, vetor, célula hospedeira e composição farmacêutica
BR112012024287A2 (pt) anticorpo, composição farmacêutica, método, ácido nucleico, vetor de expressão e célula hospedeira
BRPI0814781A2 (pt) Proteína, sequência de ácido nucleico, método para a produção de ácido itacônico, vetor, célula hospedeira, e, uso de um ácido cis-aconítico
EP2709603A4 (en) MODIFIED BIOTIN-BINDING PROTEIN, FUSION PROTEINS AND APPLICATIONS THEREWITH
SG10201710631RA (en) Artificial nucleic acid molecules for improved protein or peptide expression
BR112014002006A2 (pt) proteína de fusão, célula engenheirada para expressar a proteína de fusão, vetor, e, método para tratar um paciente com câncer
EP2791159A4 (en) MODIFIED NUCLEIC ACIDS, AND SHORT-TERM CARE USES THEREOF
BR112012029716A2 (pt) sistema, método e produto de programa de computador
BR112013010220A2 (pt) "estrtura composta, artigo e processo"
BR112013023471A2 (pt) cocristal de n-[2-(7-metóxi-1-naftil) etil]acetamina, e, processo para preparar um cocristal.
BR112012031843A2 (pt) molécula de peptídeo, molécula de ácido nucleico, vetor, célula hospedeira, método de produção de peptídeo, composição, método de produção da composição, método de detecção do comportamento de internalização do peptídeo, composição farmacêutica, uso do peptídeo, método para a prevenção e/ou tratamento e kit de partes
BR112012033339A2 (pt) métodos, aparelho e produto de programa de computador
BRPI0808104A2 (pt) Métodos para preparar e para produzir um polipeptídeo, polipeptídeo, sequência de ácido nucleico isolada, construção de ácido nucleico, vetor de expressão recombinante, e, célula hospedeira recombinante
IT1397850B1 (it) Metodo e macchina per l'etichettatura di mazzette di teli.
BRPI1013484A2 (pt) "protease, dna, vetor de expressão , célula transformada, e, método para produzir uma protease".
BR112012001462A2 (pt) processo para preparar metil mercaptano.
BR112013000225A2 (pt) ''processo''
BR112015007212A2 (pt) método para produzir l-metionina, para preparar uma molécula de ácido nucleico, para a produção de s-adenosilmetionina, para a produção de cisteína, para a produção de glutationa, para a produção de 2-oxo-4-metiltiobutanoato, enzima, proteína, molécula de ácido nucleico, vetor, célula hospedeira e uso de uma proteína
BR112014000661A2 (pt) processo, e, formulação de revestimento
BR112012030603A2 (pt) processo cosmético e conjunto cosmético
EP2556387A4 (en) METHOD, SYSTEM AND COMPUTER PROGRAM FOR CLASSIFICATION OF RFID TAGS
BRPI1014942A2 (pt) "polipeptídeo, ácido nucleico, vetor, célula hospedeira, método para produzir um polipeptídeo, composição farmacêutica, e método para tratar uma doença"
EP2644616A4 (en) PROCESS FOR PRODUCING SPINDER CANVAS PROTEIN, FUSION PROTEIN, RECOMBINANT DNA, EXPRESSION VECTOR, HOST CELL, AND PRODUCTION STRAINS

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2483 DE 07-08-2018 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.