AR083376A1 - Vector de expresion para la expresion de alto nivel de proteinas recombinantes - Google Patents

Vector de expresion para la expresion de alto nivel de proteinas recombinantes

Info

Publication number
AR083376A1
AR083376A1 ARP110103745A ARP110103745A AR083376A1 AR 083376 A1 AR083376 A1 AR 083376A1 AR P110103745 A ARP110103745 A AR P110103745A AR P110103745 A ARP110103745 A AR P110103745A AR 083376 A1 AR083376 A1 AR 083376A1
Authority
AR
Argentina
Prior art keywords
expression
high level
recombinant proteins
expression vector
vector
Prior art date
Application number
ARP110103745A
Other languages
English (en)
Inventor
Aashini Parikh
Arun Singh
Sanjeev K Mendiratta
Ajit K Gupta
Mansi Jakhade
Original Assignee
Cadila Healthcare Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cadila Healthcare Ltd filed Critical Cadila Healthcare Ltd
Publication of AR083376A1 publication Critical patent/AR083376A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente solicitud proporciona un vector de expresión para la producción de proteínas y péptidos que comprende un promotor operablemente vinculado al gen de interés, TPL (líder tripartito) y los genes VA I y II, regiones de unión de matriz (MARs)/SARs, marcador antibiótico. Dicho vector se transfecciona en una célula huésped adecuada.
ARP110103745A 2010-10-08 2011-10-11 Vector de expresion para la expresion de alto nivel de proteinas recombinantes AR083376A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN2806MU2010 2010-10-08

Publications (1)

Publication Number Publication Date
AR083376A1 true AR083376A1 (es) 2013-02-21

Family

ID=45464050

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110103745A AR083376A1 (es) 2010-10-08 2011-10-11 Vector de expresion para la expresion de alto nivel de proteinas recombinantes

Country Status (12)

Country Link
US (1) US20130244280A1 (es)
EP (1) EP2625277B1 (es)
JP (1) JP5888616B2 (es)
KR (1) KR101607734B1 (es)
AR (1) AR083376A1 (es)
AU (1) AU2011311189B2 (es)
BR (1) BR112013008459A2 (es)
CA (1) CA2813903A1 (es)
EA (1) EA201390461A1 (es)
NZ (1) NZ609903A (es)
WO (1) WO2012046255A2 (es)
ZA (1) ZA201302939B (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2617217T3 (es) * 2011-03-30 2017-06-15 Pangen Biotech Inc. Vector de expresión para células animales
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
KR20140015999A (ko) * 2012-07-27 2014-02-07 한화케미칼 주식회사 신규 ＭＡＲｓ 및 이를 이용하여 목적 단백질을 생산하는 방법
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
SG11201507230PA (en) * 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
KR101591823B1 (ko) * 2013-12-27 2016-02-04 재단법인 목암생명공학연구소 증가된 유전자 발현능을 갖는 발현벡터
KR101599138B1 (ko) * 2014-06-17 2016-03-03 한국생명공학연구원 재조합 단백질 발현 증진을 위한 유전자 절편을 포함하는 벡터 및 이의 용도
CN105274112B (zh) * 2015-11-20 2018-01-16 江南大学 一种在酸性条件下诱导表达的启动子
CN107177611B (zh) * 2017-05-23 2020-03-20 江苏康禾生物制药有限公司 编码组织型纤溶酶原激活剂的dna分子及其重组细胞株
CN107502594A (zh) * 2017-07-14 2017-12-22 中国药科大学 一种稳定表达外源性ex－4基因的骨髓源间充质干细胞株
CN113025651B (zh) * 2021-03-31 2023-03-24 重庆医科大学 靶向HBV核心启动子的药物筛选细胞模型、Triciribine及结构类似物新应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731178A (en) 1990-03-21 1998-03-24 Behringwerke Aktiengesellschaft Attachment-elements for stimulation of eukaryotic expression systems
US5888774A (en) 1994-12-19 1999-03-30 Cangene Corporation Recombinant DNA molecules and expression vectors for erythropoietin
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
DK1395669T3 (da) * 2001-01-26 2009-11-16 Selexis Sa Matriks bindingsregioner og fremgangsmåder til anvendelse af disse
EP1644508A1 (en) 2003-07-11 2006-04-12 Cytos Biotechnology AG Gene expression system
ATE471381T1 (de) 2005-03-04 2010-07-15 Celltrion Inc Expressionsvektor für tierische zelle mit wenigstens einer kopie von mar-dna-sequenzen am 3'-ende des transkriptionsterminationsbereichs eines gens sowie verfahren zur expression eines fremdgens unter verwendung des vektors
NZ592891A (en) * 2005-06-20 2013-02-22 Cadila Healthcare Ltd Expression vector and methods of producing high levels of proteins
CN101627123A (zh) * 2007-01-08 2010-01-13 米利波尔公司 消除基因扩增的高表达细胞系
EP2118270A2 (en) * 2007-01-08 2009-11-18 Millipore Corporation Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
WO2011015917A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant tnk-tpa (tenecteplase)

Also Published As

Publication number Publication date
AU2011311189A1 (en) 2013-05-30
NZ609903A (en) 2015-04-24
EA201390461A1 (ru) 2013-09-30
EP2625277B1 (en) 2017-09-06
JP5888616B2 (ja) 2016-03-22
KR20130094830A (ko) 2013-08-26
ZA201302939B (en) 2013-12-23
AU2011311189B2 (en) 2015-10-08
WO2012046255A8 (en) 2013-05-10
WO2012046255A3 (en) 2012-05-31
BR112013008459A2 (pt) 2016-06-28
CA2813903A1 (en) 2012-04-12
EP2625277A2 (en) 2013-08-14
US20130244280A1 (en) 2013-09-19
WO2012046255A2 (en) 2012-04-12
KR101607734B1 (ko) 2016-03-30
JP2013541953A (ja) 2013-11-21

Similar Documents

Publication Publication Date Title
AR083376A1 (es) Vector de expresion para la expresion de alto nivel de proteinas recombinantes
BR112012028805A2 (pt) composições de endorribonuclease e métodos de uso das mesmas.
AR121761A2 (es) Polipéptidos que presentan actividad de fosfolipasa y polinucleótidos que codifican los mismos
CO2019014704A2 (es) Agentes potenciadores para la transfección celular mejorada y/o la producción del vector raav
CL2012003077A1 (es) Polipeptido que comprende el decimo dominio de fibronectina tipo iii (10fn3), en donde el dominio 10fn3 se une al dominio 1 o 2 de seroalbumina humana, y donde la vida media del suero del polipéptido en presencia de seroalbumina es al menos 5 veces mayor que en su ausencia; acido nucleico que lo codifica; vector que lo comprende; celula hospedera que lo expresa; composición que lo comprende; y su uso para tratar la diabetes.
BRPI0924123B8 (pt) vetores adenovirais oncolíticos, usos dos mesmos, composição farmacêutica e método para produzir gmcsf em uma célula in vitro
CL2009000082A1 (es) Acido nucleico que codifica anticuerpos anti cd79a o anti cd79b para el tratamiento de tumores hematopoyeticos; vector y células huésped que los comprenden; polipeptido codificado por dicho acido nucleico, inmunoconjugado con el cuerpo.
BR112012015740A2 (pt) anticorpos de anti-flt3 e metodos de uso dos mesmos
BR122020002402B8 (pt) Proteína de ligação de antígeno isolada recombinante que liga il-23, composição farmacêutica, molécula de ácido nucleico isolada recombinante, vetor recombinante, célula hospedeira recombinante, métodos de confecção da referida proteína de ligação e uso da mesma
BR112014029441A2 (pt) rna tendo como alvo dna; polinucleotídeo de dna; vetor de expressão recombinante; célula hospedeira geneticamente modificada in vitro; polipeptídeo de modificação dirigido a sítio variante; polipeptídeo de modificação dirigido a sítio quimérico; polinucleotídeo de rna; célula modificada geneticamente; organismo não humano transgênico cujo genoma compreende um transgene; composição; método de modificação específica de sítio de um dna de alvo; método de modulação de transcrição específica de sítio dentro de um dna de alvo; método de modificação específica de sítio em dna de alvo; método de promover clivagem e modificação específica de sítio de um dna de alvo em uma célula; método de produzir uma célula modificada geneticamente em um sujeito; método de modificar dna de alvo em uma célula modificada geneticamente; kit; kit para direcionar dna de alvo; método de modular seletivamente transcrição de um dna de alvo em uma célula hospedeira; ácido nucleico isolado; célula hospedeira modificada geneticamente; e biblioteca de ácidos nucleicos
BR112014008152A2 (pt) método para produção de uma proteina de interesse cultivando uma linhagem celular eucariótica recombinante compreendendo uma construção de expressão compreendendo um promotor regulável e uma molécula de ácido nucleico que codifica uma poi sob o controle transcricional de tal promotor
CL2012000197A1 (es) Ácidos nucleicos aislados correspondientes a promotores de expresion genica, casete de expresion y celula huesped que lo comprenden, y metodo para controlar la expresion genica en celulas oclusivas vegetales.
CO6741152A2 (es) Anticuerpos estables y solubles
CL2011003075A1 (es) Proteina precursora que comprende una secuencia repetitiva con propiedades de autoensamblaje que puede ser escindida en sus componentes, los que comprenden un peptido de interes y un peptido auxiliar de autoensamblaje; acido nucleico que la codifica; casete de expresion, vector; metodo de produccion; y su uso.
PE20180674A1 (es) Uso de octacetidos sintasa para producir acido kermesico y acido flavokermesico
BR112014031526A2 (pt) promotores para expressar um gene em uma célula
BR112015027373A2 (pt) Processo de expressão
CL2012001143A1 (es) Anticuerpo anti-fractalkina (anti-fkn); composicion farmaceutica que comprende dicho anticuerpo anti-fkn; acido nucleico; vector; celula huesped; uso del anticuerpo anti-fkn para tratar trastornos inflamatorios.
AR080680A1 (es) Proteinas de union a basigina
AR116615A2 (es) Operaciones de cosecha mejoradas para proteínas recombinadas
BR112021017852A2 (pt) Vetores de dna não virais e usos dos mesmos para expressar terapêuticos de fenilalanina hidroxilase (pah)
WO2011159713A3 (en) Compositions and methods for gene expression and chromatin profiling of individual cell types within a tissue
BRPI1008762B8 (pt) métodos para seleção de células de mamífero expressando uma proteína heteróloga, para produzir um produto de interesse e uso de um meio de cultura seletivo
EA201391666A1 (ru) Рекомбинантная экспрессия растворимого интерферона
BRPI0605648A (pt) construções de dna que contém a seqüência codificante do gene hahb-10 de helianthus annuus, método para gerar plantas com ciclo de vida reduzido e com alta toleráncia a herbicidas e plantas transgênicas com dita seqüência

Legal Events

Date Code Title Description
FB Suspension of granting procedure