ATE437233T1 - Matrix-anheftungsregionen und verfahren zu deren verwendung - Google Patents

Matrix-anheftungsregionen und verfahren zu deren verwendung

Info

Publication number
ATE437233T1
ATE437233T1 AT02726395T AT02726395T ATE437233T1 AT E437233 T1 ATE437233 T1 AT E437233T1 AT 02726395 T AT02726395 T AT 02726395T AT 02726395 T AT02726395 T AT 02726395T AT E437233 T1 ATE437233 T1 AT E437233T1
Authority
AT
Austria
Prior art keywords
matrix attachment
attachment regions
relates
mar
compositions
Prior art date
Application number
AT02726395T
Other languages
English (en)
Inventor
Nicolas Mermod
Monique Zahn-Zabal
Markus Imhof
Philippe Chatellard
Pierre-Alain Girod
Original Assignee
Selexis Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Selexis Sa filed Critical Selexis Sa
Application granted granted Critical
Publication of ATE437233T1 publication Critical patent/ATE437233T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electric Connection Of Electric Components To Printed Circuits (AREA)
  • Wire Bonding (AREA)
  • Local Oxidation Of Silicon (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT02726395T 2001-01-26 2002-01-28 Matrix-anheftungsregionen und verfahren zu deren verwendung ATE437233T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26435501P 2001-01-26 2001-01-26
US28139101P 2001-04-04 2001-04-04
PCT/IB2002/002137 WO2002074969A2 (en) 2001-01-26 2002-01-28 Matrix attachment regions and methods for use thereof

Publications (1)

Publication Number Publication Date
ATE437233T1 true ATE437233T1 (de) 2009-08-15

Family

ID=26950488

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02726395T ATE437233T1 (de) 2001-01-26 2002-01-28 Matrix-anheftungsregionen und verfahren zu deren verwendung

Country Status (10)

Country Link
US (1) US7129062B2 (de)
EP (1) EP1395669B1 (de)
JP (1) JP4307079B2 (de)
AT (1) ATE437233T1 (de)
CA (1) CA2435972C (de)
DE (1) DE60233049D1 (de)
DK (1) DK1395669T3 (de)
ES (1) ES2330201T3 (de)
SG (1) SG141239A1 (de)
WO (1) WO2002074969A2 (de)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100408844B1 (ko) * 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
KR101086533B1 (ko) 2002-05-24 2011-11-23 쉐링 코포레이션 중화 사람 항-ｉｇｆｒ 항체, 이를 제조하는 방법 및 이를 포함하는 조성물
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
CA2965865C (en) 2002-07-18 2021-10-19 Merus N.V. Recombinant production of mixtures of antibodies
EP1572994B1 (de) 2002-12-20 2007-02-21 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
EP2395016A3 (de) 2003-05-30 2012-12-19 Merus B.V. Entwurf und Verwendung von gepaarten unterschiedlichen Regionen von spezifisch bindenden Molekülen
JP2005031812A (ja) * 2003-07-08 2005-02-03 Toshiba Corp 画像形成装置およびその制御方法
EP2292754B1 (de) 2003-10-24 2012-12-26 Selexis S.A. Gentransfer und Expression mit hoher Effizienz in Säugerzellen mittels eines Mehrfachtransfektionsverfahrens von Matrixbindungsbereichsequenzen
EP1694850B1 (de) 2003-11-12 2011-06-29 Schering Corporation Plasmidsystem zur expression mehrerer gene
TW200526684A (en) 2003-11-21 2005-08-16 Schering Corp Anti-IGFR1 antibody therapeutic combinations
GB0327919D0 (en) * 2003-12-02 2004-01-07 Plant Bioscience Ltd Enhanced expression
DK1737971T3 (da) 2004-01-20 2017-11-13 Merus Nv Blandinger af bindingsproteiner
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
SI1809750T1 (sl) 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
JP5291341B2 (ja) 2004-11-08 2013-09-18 クロマジェニックス ベー ヴェー タンパク質を高レベルで発現する宿主細胞の選定
PT1828249E (pt) * 2004-12-03 2011-02-25 Schering Corp Biomarcadores para a pré-selecção de pacientes para terapêutica anti-igf1r
WO2007021353A2 (en) * 2005-06-10 2007-02-22 Genentech, Inc. Improving antibody expression using vectors containing insulator elements
US7498150B2 (en) * 2005-08-11 2009-03-03 Centocor, Inc. Materials and methods to increase peptide chain expression
EP1996705B1 (de) 2006-03-20 2011-08-31 ChromaGenics B.V. Die expression verstärkende dna-fragmente, deren verwendung und verfahren zum finden dieser
US8058027B2 (en) * 2007-01-08 2011-11-15 Millipore Corporation Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
JP2010515435A (ja) * 2007-01-08 2010-05-13 ミリポア・コーポレイション 遺伝子増幅を不要とする高発現細胞株
JP5432117B2 (ja) * 2007-03-30 2014-03-05 アッヴィ・インコーポレイテッド 宿主細胞中の組換えタンパク質の発現を増進するための組換え発現ベクター要素（ｒＥＶＥ）
EP4206231A1 (de) 2007-12-24 2023-07-05 ID Biomedical Corporation of Quebec Rekombinante rsv-antigene
CN105274072A (zh) 2008-01-18 2016-01-27 生物马林药物股份有限公司 制备活性高度磷酸化人溶酶体硫酸酯酶及其应用
BRPI0914484A2 (pt) * 2008-10-28 2015-09-01 Avesthagen Ltd Vetor de expressão e processos deste
WO2010081494A1 (en) 2009-01-19 2010-07-22 King Faisal Specialist Hospital And Research Centre A method of increasing protein expression in eukaryotic cells
WO2010081890A1 (en) 2009-01-19 2010-07-22 Innate Pharma Anti-kir3d antibodies
WO2010149745A1 (en) 2009-06-24 2010-12-29 Glaxosmithkline Biologicals S.A. Recombinant rsv antigens
MX2012000036A (es) 2009-06-24 2012-02-28 Glaxosmithkline Biolog Sa Vacuna.
HRP20220756T1 (hr) 2009-07-15 2022-09-02 Glaxosmithkline Biologicals S.A. Proteinski pripravci rsv f i postupci za izradu istih
WO2011015916A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant infliximab
WO2011015924A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant trastuzumab
WO2011015917A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant tnk-tpa (tenecteplase)
WO2011015918A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant cetuximab
KR20120099376A (ko) 2009-09-18 2012-09-10 셀렉시스 에스. 에이. 강화된 전이 유전자 발현과 공정 방법 및 그 산물
AR082319A1 (es) 2010-07-22 2012-11-28 Biomarin Pharm Inc Produccion de una n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada y sus usos
NZ609903A (en) 2010-10-08 2015-04-24 Cadila Healthcare Ltd Expression vector for high level expression of recombinant proteins
WO2012095514A1 (en) 2011-01-14 2012-07-19 Vivalis Recombinant protein production system
BR112014006822B1 (pt) 2011-09-22 2021-05-18 Amgen Inc. Proteína de ligação ao antígeno cd27l, composição compreendendo uma proteína de ligação ao antígeno cd27l, ácido nucleico isolado, vetor de expressão, célula hospedeira recombinante procariótica, método de preparação de um conjugado de droga e anticorpo cd27l e uso de uma proteína de ligação ao antígeno cd27l
AU2013249985B2 (en) 2012-04-20 2017-11-23 Merus N.V. Methods and means for the production of Ig-like molecules
AR091069A1 (es) 2012-05-18 2014-12-30 Amgen Inc Proteinas de union a antigeno dirigidas contra el receptor st2
UY35148A (es) 2012-11-21 2014-05-30 Amgen Inc Immunoglobulinas heterodiméricas
KR102235603B1 (ko) 2013-02-01 2021-04-05 셀렉시스 에스. 에이. 향상된 이식유전자 발현 및 가공
US9580486B2 (en) 2013-03-14 2017-02-28 Amgen Inc. Interleukin-2 muteins for the expansion of T-regulatory cells
US9708375B2 (en) 2013-03-15 2017-07-18 Amgen Inc. Inhibitory polypeptides specific to WNT inhibitors
EP3004167B1 (de) 2013-05-30 2018-07-25 Kiniksa Pharmaceuticals, Ltd. Oncostatin-m-rezeptor-antigenbindende proteine
US11427627B2 (en) 2013-09-05 2022-08-30 Amgen Inc. Fc-containing molecules exhibiting predictable, consistent, and reproducible glycoform profiles
WO2015060935A1 (en) 2013-10-22 2015-04-30 Lubris, Llc Control of rheological properties of mixed hyaluronate/lubricin solutions
WO2016164937A2 (en) 2015-04-10 2016-10-13 Amgen Inc. Interleukin-2 muteins for the expansion of t-regulatory cells
BR112017021696A2 (pt) 2015-04-10 2018-07-10 Amgen Inc muteínas de interleucina-2 para a expansão de células t regulatórias
KR102668727B1 (ko) 2015-04-24 2024-05-28 제넨테크, 인크. 다중특이적 항원-결합 단백질
EP3699269A1 (de) 2015-09-22 2020-08-26 F. Hoffmann-La Roche AG Expression von fc-haltigen proteinen
EP3359576A4 (de) 2015-10-08 2019-08-28 Zymeworks Inc. Antigenbindende polypeptidkonstrukte mit kappa- und lambda-leichtketten und verwendungen davon
EP3925970A1 (de) 2015-11-02 2021-12-22 F. Hoffmann-La Roche AG Verfahren zur herstellung von fucosylierten und afucosylierten formen eines proteins
EP3293263A1 (de) * 2016-09-07 2018-03-14 Steinbeis Innovation gGmbH Induzierbare genexpression durch lineare at-reiche dna-fragmente in säugetierzellen und zugehörige verfahren
RU2019142330A (ru) 2017-06-30 2021-07-30 Займворкс, Инк. Стабилизированные химерные fab
WO2020084528A1 (en) 2018-10-24 2020-04-30 Selexis Sa Expression systems, recombinant cells and uses thereof
AR119769A1 (es) 2019-08-13 2022-01-12 Amgen Inc Muteínas de interleucina-2 para la expansión de células t reguladoras
AU2020407208A1 (en) 2019-12-17 2022-06-02 Amgen Inc. Dual interleukin-2 /tnf receptor agonist for use in therapy
JP2023515504A (ja) 2020-02-24 2023-04-13 ノバルティス アーゲー 組み換え産生されたポリペプチドの精製

Family Cites Families (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8925590D0 (en) * 1989-11-13 1990-01-04 Central Blood Lab Authority Monoclonal antibodies
US5733761A (en) * 1991-11-05 1998-03-31 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6063630A (en) * 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
DE69331908T2 (de) 1992-10-05 2002-12-12 North Carolina State University, Raleigh Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen
US5610053A (en) * 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5464758A (en) * 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5888981A (en) * 1993-06-14 1999-03-30 Basf Aktiengesellschaft Methods for regulating gene expression
US5907078A (en) * 1994-12-09 1999-05-25 Greenberg; Norman M. Transgenic mouse model for prostate cancer
DE19541450C2 (de) * 1995-11-07 1997-10-02 Gsf Forschungszentrum Umwelt Genkonstrukt und dessen Verwendung
US6596514B2 (en) * 1996-01-11 2003-07-22 Immunex Corporation Expression augmenting sequence elements (EASE) for eukaryotic expression systems
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
AU2064297A (en) * 1996-02-29 1997-09-16 Immusol, Inc Hepatitis c virus ribozymes
NZ333188A (en) * 1996-06-06 2001-06-29 Systemix Inc Use of DNA scaffold attachments regions to modify expression of a heterologous gene
EP1471144B1 (de) 1996-12-02 2010-06-23 Genaera Corporation Biologische Variabilität von Asthma-assoziiertem Faktor 2 AAF2 (IL-9 Rezeptor) nützlich für die Behandlung und Diagnose von atopischen Allergien wie Asthma und Asthma-ähnlichen Erkrankungen
US6245974B1 (en) 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
AU734476B2 (en) * 1997-11-05 2001-06-14 Baylor College Of Medicine Sequences for targeting metastatic cells
US6635806B1 (en) * 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
KR100795626B1 (ko) 1998-07-21 2008-01-17 코브라 바이오매뉴팩쳐링 피엘씨. 도처에 있는 염색질 개방 요소(유씨오이)를 포함하는폴리뉴클레오티드
CA2343080A1 (en) * 1998-09-29 2000-04-06 Pioneer Hi-Bred International, Inc. Mar/sar elements flanking rsyn7-driven construct
DE19848017A1 (de) * 1998-10-17 2000-04-20 Multigene Biotech Gmbh Episomal replizierender Vektor zur Expression eines Transgens in Säugerzellen
ATE279524T1 (de) 1998-12-01 2004-10-15 Dow Agrosciences Llc Künstliche matrix-anheftungsregion zur erhöhung der expression von in pflanzen eingeführten genen
US6225113B1 (en) * 1998-12-04 2001-05-01 Genvec, Inc. Use of trans-activation and cis-activation to modulate the persistence of expression of a transgene in an at least E4-deficient adenovirus
US6207879B1 (en) * 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
US6232526B1 (en) * 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
US6429357B1 (en) * 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6194636B1 (en) * 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
CA2374365A1 (en) * 1999-05-28 2000-12-07 Sangamo Biosciences, Inc. Gene switches
US20050129669A1 (en) * 1999-06-21 2005-06-16 Transkaryotic Therapies, Inc., A Massachusetts Corporation DNA construct for effecting homologous recombination and uses thereof
US6972349B1 (en) * 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
JP3507858B2 (ja) * 1999-11-19 2004-03-15 奈良先端科学技術大学院大学長 植物において外来遺伝子の発現を安定化させるｄｎａ断片、外来遺伝子を植物において安定に発現させる遺伝子導入方法、外来遺伝子を安定に発現させた形質転換植物
AU7521500A (en) 1999-11-30 2001-06-12 Novartis Ag Increased transgene expression in retroviral vectors having scaffold attachment region
US6864085B2 (en) 1999-12-14 2005-03-08 Novartis Ag Bovine immunodeficiency virus (BIV) based vectors
US20020127199A1 (en) 2000-02-03 2002-09-12 Tang Y. Tom Novel nucleic acids and polypeptides
US6821775B1 (en) * 2000-02-11 2004-11-23 Genvec, Inc. Viral vector encoding pigment epithelium-derived factor
DE10006886A1 (de) 2000-02-16 2001-08-23 Hepavec Ag Fuer Gentherapie Nichthumaner helferabhängiger Virusvektor
US6569681B1 (en) * 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
US6747189B1 (en) * 2000-03-21 2004-06-08 Dekalb Genetics Corporation Maize glycine rich protein promoter compositions and methods for use thereof
US6730826B2 (en) * 2000-03-22 2004-05-04 University Of Kentucky Research Foundation Plant trichome gland specific promoter sequence
US20030157715A1 (en) * 2000-06-26 2003-08-21 Ulrich Laemmli Modulation of chromosome function by chromatin remodeling agents
AU2001279744A1 (en) 2000-06-26 2002-01-08 Universite De Geneve Modulation of chromosome function by chromatin remodeling agents
CA2416877C (en) 2000-07-27 2013-03-12 Apogene Gmbh & Co. Kg Somatic cloning gene transfer for producing recombinant proteins, cells and organs
KR100408844B1 (ko) * 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
EP1313868B1 (de) * 2000-08-30 2006-07-19 North Carolina State University Transgene pflanzen, die molekulare decoys enthalten, die den proteininhalt ändern
EP1322771A2 (de) * 2000-09-09 2003-07-02 BASF Plant Science GmbH Modifiziertes tet-induzierbares system zur regulierung der genexpression in pflanzen
GB0022995D0 (en) * 2000-09-20 2000-11-01 Cobra Therapeutics Ltd Polynucleotide
US6919204B2 (en) * 2000-09-29 2005-07-19 Sangamo Biosciences, Inc. Modulation of gene expression using localization domains
US7405276B2 (en) * 2000-11-01 2008-07-29 Elusys Therapeutics, Inc. Method of producing bispecific molecules by protein trans-splicing
DE10061150A1 (de) * 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
AU2002216443A1 (en) * 2000-12-15 2002-06-24 Pangen Biotech Inc. Expression vector for animal cell containing nuclear matrix attachment region fointerferon beta
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
DE10109780A1 (de) 2001-02-28 2002-09-05 Cardion Ag Episomale Vektoren enthaltend Matrix-Anheftungsregionen zur zellspezifischen Genexpression
EP1365801A4 (de) 2001-03-08 2004-10-13 Nuvelo Inc Verfahren und materialien in verbindung mit fibulinartigen polypeptiden und polynucleotiden
JP4817514B2 (ja) * 2001-03-09 2011-11-16 協和発酵キリン株式会社 新規動物細胞用ベクターおよびその使用
US20040076954A1 (en) * 2001-03-12 2004-04-22 Irm, Llc Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
ATE397084T1 (de) * 2001-03-13 2008-06-15 Novartis Pharma Gmbh Lentivirale verpackungskonstrukte
WO2002079447A2 (en) * 2001-03-30 2002-10-10 Avigenics, Inc. Avian lysozyme promoter
DE10129010A1 (de) * 2001-06-13 2002-12-19 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
US20040126189A1 (en) * 2001-06-27 2004-07-01 Chowdhury Ajit K. Method for stabilizing chromium-contaminated materials
US20030032597A1 (en) * 2001-07-31 2003-02-13 Sebestyen Magdolna G. Targeting nucleic acids to a cellular nucleus
US7122715B2 (en) * 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
AU2002341795A1 (en) 2001-09-21 2003-04-01 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
US7138278B2 (en) * 2001-11-20 2006-11-21 Monsanto Technology, L.L.C. Maize cytoplasmic glutamine synthetase promoter compositions and methods for use thereof
FR2832423B1 (fr) 2001-11-22 2004-10-08 Vivalis Systeme d'expression de proteines exogenes dans un systeme aviaire
US6875588B2 (en) * 2001-11-30 2005-04-05 Avigenics, Inc. Ovomucoid promoter and methods of use
CA2427190A1 (en) * 2002-04-30 2003-10-30 Alberta Research Council Inc. Production of recombinant epidermal growth factor in plants
US20030224477A1 (en) * 2002-05-31 2003-12-04 Heartlein Michael W. Optimized promoter constructs
US7262025B2 (en) * 2002-06-18 2007-08-28 Zymogenetics, Inc. Hybrid vector having a cytomegalovirus enhancer and myeloproliferative sarcoma virus promoter
US20040016015A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US6933136B2 (en) * 2002-09-20 2005-08-23 Novo Nordisk A/S Method for making recombinant proteins
CN1263860C (zh) * 2002-09-30 2006-07-12 华南农业大学 多基因载体的构建方法与应用
WO2004053106A2 (en) 2002-12-05 2004-06-24 Regulome Corporation Profiled regulatory sites useful for gene control
WO2004053137A2 (en) 2002-12-11 2004-06-24 Cytos Biotechnology Ag Method for protein production
AU2002953381A0 (en) 2002-12-18 2003-01-09 Diatech Pty Ltd In vivo affinity maturation scheme
EP1445320A1 (de) 2003-02-05 2004-08-11 ARTEMIS Pharmaceuticals GmbH Automatisches Gen-Targeting mittels nicht toxisischer nachweissbarer Marker
US7585963B2 (en) 2003-04-15 2009-09-08 Synageva Biopharma Corp. Chicken ovalbumin nucleotide sequence
EP2395016A3 (de) 2003-05-30 2012-12-19 Merus B.V. Entwurf und Verwendung von gepaarten unterschiedlichen Regionen von spezifisch bindenden Molekülen
WO2005005644A1 (en) * 2003-07-11 2005-01-20 Cytos Biotechnology Ag Gene expression system
WO2005021765A2 (en) 2003-08-27 2005-03-10 Orf Liftaekni Hf. Enhancing accumulation of heterologous polypeptides in plant seeds through targeted suppression of endogenous storage proteins
ES2354160T3 (es) 2003-10-21 2011-03-10 Merck Serono Sa Secuencia de adn mínima que actúa como un aislante de cromatina, y su uso en la expresión de proteínas.

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EP1395669A2 (de) 2004-03-10
US7129062B2 (en) 2006-10-31
ES2330201T3 (es) 2009-12-07
JP4307079B2 (ja) 2009-08-05
WO2002074969A2 (en) 2002-09-26
WO2002074969A3 (en) 2003-12-24
EP1395669B1 (de) 2009-07-22
CA2435972C (en) 2011-09-13
DE60233049D1 (de) 2009-09-03
DK1395669T3 (da) 2009-11-16
US20030087342A1 (en) 2003-05-08
JP2004519246A (ja) 2004-07-02
CA2435972A1 (en) 2002-09-26
SG141239A1 (en) 2008-04-28

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