ATE429500T1 - Stellenspezifischer einbau von ketoaminosäuren in proteine - Google Patents

Stellenspezifischer einbau von ketoaminosäuren in proteine

Info

Publication number
ATE429500T1
ATE429500T1 AT03809019T AT03809019T ATE429500T1 AT E429500 T1 ATE429500 T1 AT E429500T1 AT 03809019 T AT03809019 T AT 03809019T AT 03809019 T AT03809019 T AT 03809019T AT E429500 T1 ATE429500 T1 AT E429500T1
Authority
AT
Austria
Prior art keywords
proteins
site
orthogonal
methods
specific incorporation
Prior art date
Application number
AT03809019T
Other languages
English (en)
Inventor
Peter Schultz
Lei Wang
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Application granted granted Critical
Publication of ATE429500T1 publication Critical patent/ATE429500T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT03809019T 2002-10-16 2003-10-15 Stellenspezifischer einbau von ketoaminosäuren in proteine ATE429500T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41926502P 2002-10-16 2002-10-16
US42099002P 2002-10-23 2002-10-23
PCT/US2003/032576 WO2004035743A2 (en) 2002-10-16 2003-10-15 Site specific incorporation of keto amino acids into proteins

Publications (1)

Publication Number Publication Date
ATE429500T1 true ATE429500T1 (de) 2009-05-15

Family

ID=32110229

Family Applications (2)

Application Number Title Priority Date Filing Date
AT09003609T ATE526414T1 (de) 2002-10-16 2003-10-15 Ortsspezifische einbindung von ketoaminosäuren in proteine
AT03809019T ATE429500T1 (de) 2002-10-16 2003-10-15 Stellenspezifischer einbau von ketoaminosäuren in proteine

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT09003609T ATE526414T1 (de) 2002-10-16 2003-10-15 Ortsspezifische einbindung von ketoaminosäuren in proteine

Country Status (16)

Country Link
US (5) US7432092B2 (de)
EP (2) EP2062976B1 (de)
JP (1) JP4444113B2 (de)
KR (1) KR20050072441A (de)
AT (2) ATE526414T1 (de)
AU (2) AU2003277373B2 (de)
CA (1) CA2502029C (de)
CY (1) CY1110483T1 (de)
DE (1) DE60327369D1 (de)
DK (1) DK1578949T3 (de)
ES (1) ES2324608T3 (de)
IL (3) IL167715A (de)
MX (1) MXPA05003983A (de)
PT (1) PT1578949E (de)
SI (1) SI1578949T1 (de)
WO (1) WO2004035743A2 (de)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005502322A (ja) * 2001-04-19 2005-01-27 ザ スクリップス リサーチ インスティテュート 非天然アミノ酸のインビボ組込み
EP1558747B1 (de) 2002-10-16 2009-10-14 The Scripps Research Institute Glycoproteinsynthese
CN103215227B (zh) 2003-04-17 2015-09-16 斯克利普斯研究院 扩展真核生物遗传密码
JP5275566B2 (ja) 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
ES2301099T3 (es) * 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
WO2005038002A2 (en) 2003-10-14 2005-04-28 The Scripps Research Institute Site-specific incorporation of redox active amino acids into proteins
NZ548255A (en) 2004-02-02 2010-10-29 Ambrx Inc Modified human interferon polypeptides and their uses
MXPA06013586A (es) * 2004-05-25 2007-11-09 Scripss Res Inst Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.
KR20070034512A (ko) 2004-06-18 2007-03-28 암브룩스, 인코포레이티드 신규 항원-결합 폴리펩티드 및 이의 용도
AU2009212311B8 (en) 2004-07-21 2013-05-30 Ambrx, Inc. Modified leptin polypeptides and their uses
EP1771573A4 (de) * 2004-07-21 2009-02-18 Ambrx Inc Nichtnatürliche kodierte aminosäuren verwendende biosynthetische polypeptide
CN101151366B (zh) 2004-09-21 2012-12-19 斯克利普斯研究院 将炔基氨基酸体内掺入真细菌的蛋白
EP1794312A4 (de) * 2004-09-22 2009-08-12 Scripps Research Inst Ortsspezifische etikettierung von proteinen für nmr-studien
EP1809758A4 (de) 2004-10-20 2009-04-01 Scripps Research Inst Stellenspezifischer in-vivo-einbau von n-acetylgalactosamin-aminosäuren in eubakterien
US8216804B2 (en) * 2004-10-27 2012-07-10 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
ATE541934T1 (de) 2004-12-22 2012-02-15 Ambrx Inc Zusammensetzungen von aminoacyl-trna-synthetase und verwendungen davon
CN104803865A (zh) 2004-12-22 2015-07-29 Ambrx公司 含有非天然氨基酸和多肽的组合物、涉及非天然氨基酸和多肽的方法以及其用途
NZ555386A (en) 2004-12-22 2011-01-28 Ambrx Inc Formulations of human growth hormone comprising a non-naturally encoded amino acid
JP2008525473A (ja) 2004-12-22 2008-07-17 アンブレツクス・インコーポレイテツド 修飾されたヒト成長ホルモン
WO2006073846A2 (en) 2004-12-22 2006-07-13 Ambrx, Inc. Methods for expression and purification of recombinant human growth hormone
MX2007015058A (es) 2005-06-03 2008-01-28 Ambrx Inc Moleculas de interferon humano mejoradas y sus usos.
US20090306023A1 (en) * 2005-06-29 2009-12-10 Ramirez Jose E Stable organic peroxide compositions
AU2006304110B2 (en) 2005-10-12 2012-02-02 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
CN101454461A (zh) 2005-11-16 2009-06-10 Ambrx公司 包括非天然氨基酸的方法和组合物
NZ568578A (en) 2005-12-14 2011-10-28 Ambrx Inc Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
US7776535B2 (en) * 2006-02-06 2010-08-17 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
CN101400796B (zh) 2006-03-09 2013-03-27 斯克利普斯研究院 在真细菌宿主细胞内表达正交翻译组分的系统
RU2464315C2 (ru) * 2006-03-16 2012-10-20 Зе Скрипс Ресеч Инститьют Генетически запрограммированная экспрессия белков, содержащих не встречающуюся в природе аминокислоту фенилселеноцистеин
US7807411B2 (en) * 2006-05-23 2010-10-05 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
CN106008699A (zh) 2006-09-08 2016-10-12 Ambrx公司 经修饰的人类血浆多肽或Fc骨架和其用途
AU2007292892B9 (en) * 2006-09-08 2013-02-28 Ambrx, Inc. Hybrid suppressor tRNA for vertebrate cells
AU2007292893B2 (en) 2006-09-08 2012-03-01 Ambrx, Inc. Suppressor tRNA transcription in vertebrate cells
EP2052083B1 (de) * 2006-09-21 2014-01-08 The Scripps Research Institute Genetisch programmierte expression von selektiv sulfatierten proteinen in eubakterien
WO2008049034A1 (en) * 2006-10-17 2008-04-24 Zymera, Inc. System for assays of aminotranferase
EP2069481A4 (de) 2006-10-18 2010-01-06 Scripps Research Inst Genetischer einbau unnatürlicher aminosäuren in proteine in säugerzellen
GB0623974D0 (en) * 2006-11-30 2007-01-10 Medical Res Council Evolved orthogonal ribosomes
EP2068909B1 (de) 2007-03-30 2012-04-25 Ambrx, Inc. Modifizierte fgf-21 polypeptide und ihre verwendung
CN101675071B (zh) 2007-05-02 2014-06-18 Ambrx公司 经修饰干扰素β多肽和其用途
ES2632504T3 (es) 2007-11-20 2017-09-13 Ambrx, Inc. Polipéptidos de insulina modificados y sus usos
CN106928339A (zh) 2008-07-23 2017-07-07 Ambrx 公司 经修饰的牛g‑csf多肽和其用途
PT2337846T (pt) 2008-09-26 2018-04-05 Ambrx Inc Vacinas e microrganismos dependentes da replicação de aminoácidos não naturais
CA2737026C (en) 2008-09-26 2019-12-24 Ambrx, Inc. Modified animal erythropoietin polypeptides and their uses
WO2011073738A1 (en) 2009-12-14 2011-06-23 Metabolic Explorer Use of inducible promoters in the production of methionine
NZ600361A (en) 2009-12-21 2014-06-27 Ambrx Inc Modified bovine somatotropin polypeptides and their uses
EP2516455A4 (de) 2009-12-21 2013-05-22 Ambrx Inc Modifizierte schweine-somatotropin-polypeptide und deren verwendungen
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
EP2605789B1 (de) 2010-08-17 2019-06-05 Ambrx, Inc. Modifizierte relaxinpolypeptide und ihre verwendungen
AR083006A1 (es) 2010-09-23 2013-01-23 Lilly Co Eli Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas
CN102888387B (zh) * 2011-07-21 2015-03-18 中国科学院生物物理研究所 3-氯代酪氨酸翻译系统及其应用
UY35368A (es) 2013-03-08 2014-10-31 Irm Llc Péptidos y composiciones para el tratamiento de daño articular
CA2901928C (en) 2013-03-13 2017-10-10 Eli Lilly And Company Modified canine leptin polypeptides
WO2015009948A1 (en) 2013-07-19 2015-01-22 Regents Of The University Of California Milk fat globule epidermal growth factor 8 regulates fatty acid uptake
US10011649B2 (en) 2013-08-26 2018-07-03 Arizona Board Of Regents On Behalf Of Arizona State University High affinity synbodies for influenza
EP3164501A4 (de) 2014-07-02 2018-01-17 Sutro Biopharma, Inc. Auxotrophes escherichia coli mit hoher wachstumskapazität und verfahren zur verwendung
KR102854572B1 (ko) 2014-10-24 2025-09-02 브리스톨-마이어스 스큅 컴퍼니 변형된 fgf-21 폴리펩티드 및 그의 용도
CN107406463A (zh) 2014-12-04 2017-11-28 细胞基因公司 生物分子共轭物
US9744624B2 (en) * 2015-06-17 2017-08-29 Kinsus Interconnect Technology Corp. Method for manufacturing circuit board
KR20190084287A (ko) 2016-11-14 2019-07-16 노파르티스 아게 연골 손상 및 관절염 치료 방법 및 조성물
CN109996809A (zh) 2016-11-14 2019-07-09 诺华股份有限公司 与促融合蛋白minion相关的组合物、方法和治疗用途
BR112019016139A2 (pt) 2017-02-08 2020-04-07 Bristol-Myers Squibb Company polipeptídio de relaxina modificada compreendendo um melhorador farmacocinético e usos do mesmo
TW202128996A (zh) * 2019-10-10 2021-08-01 美商史基普研究協會 用於活體內合成非天然多肽的組合物及方法
WO2024077277A1 (en) 2022-10-07 2024-04-11 Ambrx, Inc. Drug linkers and antibody conjugates thereof
WO2024091824A1 (en) 2022-10-26 2024-05-02 Ada Forsyth Institute, Inc. Differentiation and reprogramming of chondrocyte
KR20250134670A (ko) 2023-01-16 2025-09-11 암브룩스, 인코포레이티드 항-cd70 항체-약물 접합체
WO2024178310A1 (en) 2023-02-23 2024-08-29 Ambrx, Inc. Trop2-directed antibody-drug conjugates and uses thereof
WO2024241086A1 (en) 2023-05-24 2024-11-28 Ambrx, Inc. Pegylated bovine interferon lambda and methods of use thereof
WO2025041055A1 (en) 2023-08-22 2025-02-27 Ambrx, Inc. Anti-psma adc conjugate compositions and methods of use thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2219136A1 (en) 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US5756316A (en) 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US5783431A (en) 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
JP2005502322A (ja) * 2001-04-19 2005-01-27 ザ スクリップス リサーチ インスティテュート 非天然アミノ酸のインビボ組込み
EP1558747B1 (de) * 2002-10-16 2009-10-14 The Scripps Research Institute Glycoproteinsynthese

Also Published As

Publication number Publication date
AU2009203098B2 (en) 2011-09-08
JP2006507814A (ja) 2006-03-09
EP1578949B1 (de) 2009-04-22
AU2009203098A1 (en) 2009-08-20
US8071344B2 (en) 2011-12-06
US8114629B2 (en) 2012-02-14
MXPA05003983A (es) 2005-06-22
US20090215117A1 (en) 2009-08-27
WO2004035743A2 (en) 2004-04-29
JP4444113B2 (ja) 2010-03-31
US7524647B2 (en) 2009-04-28
IL167715A (en) 2010-11-30
EP1578949A2 (de) 2005-09-28
SI1578949T1 (sl) 2009-08-31
WO2004035743A8 (en) 2005-06-09
US20080227153A1 (en) 2008-09-18
EP2062976B1 (de) 2011-09-28
US20060068478A1 (en) 2006-03-30
AU2003277373A1 (en) 2004-05-04
DK1578949T3 (da) 2009-07-27
PT1578949E (pt) 2009-07-14
EP2062976A3 (de) 2010-04-07
US7432092B2 (en) 2008-10-07
EP2062976A2 (de) 2009-05-27
US7910345B2 (en) 2011-03-22
CA2502029C (en) 2014-06-10
AU2003277373B2 (en) 2009-05-07
DE60327369D1 (de) 2009-06-04
EP1578949A4 (de) 2006-12-20
KR20050072441A (ko) 2005-07-11
US20080176284A1 (en) 2008-07-24
CA2502029A1 (en) 2004-04-29
IL210359A0 (en) 2011-03-31
CY1110483T1 (el) 2015-04-29
IL201208A (en) 2011-02-28
US20110318806A1 (en) 2011-12-29
ATE526414T1 (de) 2011-10-15
WO2004035743A3 (en) 2005-11-10
ES2324608T3 (es) 2009-08-11
HK1083351A1 (en) 2006-06-30

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