ATE429500T1 - Stellenspezifischer einbau von ketoaminosäuren in proteine - Google Patents

Stellenspezifischer einbau von ketoaminosäuren in proteine

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Publication number
ATE429500T1
ATE429500T1 AT03809019T AT03809019T ATE429500T1 AT E429500 T1 ATE429500 T1 AT E429500T1 AT 03809019 T AT03809019 T AT 03809019T AT 03809019 T AT03809019 T AT 03809019T AT E429500 T1 ATE429500 T1 AT E429500T1
Authority
AT
Austria
Prior art keywords
proteins
site
orthogonal
methods
specific incorporation
Prior art date
Application number
AT03809019T
Other languages
English (en)
Inventor
Peter Schultz
Lei Wang
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Application granted granted Critical
Publication of ATE429500T1 publication Critical patent/ATE429500T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03809019T 2002-10-16 2003-10-15 Stellenspezifischer einbau von ketoaminosäuren in proteine ATE429500T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41926502P 2002-10-16 2002-10-16
US42099002P 2002-10-23 2002-10-23
PCT/US2003/032576 WO2004035743A2 (en) 2002-10-16 2003-10-15 Site specific incorporation of keto amino acids into proteins

Publications (1)

Publication Number Publication Date
ATE429500T1 true ATE429500T1 (de) 2009-05-15

Family

ID=32110229

Family Applications (2)

Application Number Title Priority Date Filing Date
AT03809019T ATE429500T1 (de) 2002-10-16 2003-10-15 Stellenspezifischer einbau von ketoaminosäuren in proteine
AT09003609T ATE526414T1 (de) 2002-10-16 2003-10-15 Ortsspezifische einbindung von ketoaminosäuren in proteine

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT09003609T ATE526414T1 (de) 2002-10-16 2003-10-15 Ortsspezifische einbindung von ketoaminosäuren in proteine

Country Status (16)

Country Link
US (5) US7432092B2 (de)
EP (2) EP2062976B1 (de)
JP (1) JP4444113B2 (de)
KR (1) KR20050072441A (de)
AT (2) ATE429500T1 (de)
AU (2) AU2003277373B2 (de)
CA (1) CA2502029C (de)
CY (1) CY1110483T1 (de)
DE (1) DE60327369D1 (de)
DK (1) DK1578949T3 (de)
ES (1) ES2324608T3 (de)
IL (3) IL167715A (de)
MX (1) MXPA05003983A (de)
PT (1) PT1578949E (de)
SI (1) SI1578949T1 (de)
WO (1) WO2004035743A2 (de)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2322631T3 (en) * 2001-04-19 2015-01-12 Scripps Research Inst Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA syntetasepar
CA2500653A1 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
MXPA05011127A (es) 2003-04-17 2006-03-10 Scripps Research Inst Expansion del codigo genetico eucariontico.
JP5275566B2 (ja) 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
EP1658366A4 (de) 2003-07-07 2006-08-09 Scripps Research Inst Zusammensetzungen von orthogonalen glutamyl-trna- und aminoacyl-trna-synthetasepaaren und deren verwendungen
AU2004267359B2 (en) * 2003-07-07 2010-09-09 The Scripps Research Institute Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
AU2004282577B2 (en) 2003-10-14 2009-10-29 The Scripps Research Institute Site-specific incorporation of redox active amino acids into proteins
WO2005074546A2 (en) 2004-02-02 2005-08-18 Ambrx, Inc. Modified human growth hormone polypeptides and their uses
CA2567624A1 (en) 2004-05-25 2005-12-08 The Scripps Research Institute Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
NZ582684A (en) 2004-06-18 2011-05-27 Ambrx Inc Use of an antibody or binding fragment thereof comprising a non naturally encoded amino acid coupled to a linker
JP2008507280A (ja) * 2004-07-21 2008-03-13 アンブレツクス・インコーポレイテツド 非天然コードアミノ酸を用いた生合成ポリペプチド
CA2580840A1 (en) 2004-09-21 2006-03-30 The Scripps Research Institute In vivo incorporation of alkynyl amino acids into proteins in eubacteria
US20060110784A1 (en) * 2004-09-22 2006-05-25 The Scripps Research Institute Site-specific labeling of proteins for NMR studies
EP1809758A4 (de) 2004-10-20 2009-04-01 Scripps Research Inst Stellenspezifischer in-vivo-einbau von n-acetylgalactosamin-aminosäuren in eubakterien
WO2006110182A2 (en) * 2004-10-27 2006-10-19 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
WO2006073846A2 (en) 2004-12-22 2006-07-13 Ambrx, Inc. Methods for expression and purification of recombinant human growth hormone
MX2007007587A (es) 2004-12-22 2007-12-11 Ambrx Inc Formulaciones de la hormona del crecimiento humano que comprenden un aminoacido codificado de manera no natural.
CA2590590C (en) 2004-12-22 2016-04-19 Ambrx, Inc. Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
ATE541934T1 (de) 2004-12-22 2012-02-15 Ambrx Inc Zusammensetzungen von aminoacyl-trna-synthetase und verwendungen davon
KR20070090023A (ko) 2004-12-22 2007-09-04 암브룩스, 인코포레이티드 변형 인간 성장 호르몬
KR20080026135A (ko) 2005-06-03 2008-03-24 암브룩스, 인코포레이티드 개선된 인간 인터페론 분자 및 이의 용도
US20090306023A1 (en) * 2005-06-29 2009-12-10 Ramirez Jose E Stable organic peroxide compositions
BRPI0617191A2 (pt) * 2005-10-12 2011-07-19 Scripps Research Inst modificação pós-traducional de polipeptìdeos expressos em fagos
EP1951890A4 (de) 2005-11-16 2009-06-24 Ambrx Inc Verfahren und zusammensetzungen mit nichtnatürlichen aminosäuren
JP2009519942A (ja) 2005-12-14 2009-05-21 アンブルックス，インコーポレイテッド 非天然アミノ酸およびポリペプチドを含んでいる組成物、それらに関する方法、ならびに、それらの使用
US7776535B2 (en) * 2006-02-06 2010-08-17 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
JP5589186B2 (ja) 2006-03-09 2014-09-17 ザ スクリプス リサーチ インスティテュート 真正細菌宿主細胞における直交翻訳成分の発現システム
US8217145B2 (en) * 2006-03-16 2012-07-10 The Scrips Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
RU2473690C2 (ru) * 2006-05-23 2013-01-27 Зе Скрипс Ресеч Инститьют Генетически кодируемые флюоресцентные кумариновые аминокислоты
ES2457527T3 (es) * 2006-09-08 2014-04-28 Ambrx, Inc. ARNt Supresor hibrido en células de vertebrados
PT2064333E (pt) 2006-09-08 2014-06-09 Ambrx Inc Supressor híbrido arnt para células de vertebrados
CN104193815A (zh) 2006-09-08 2014-12-10 Ambrx公司 经修饰的人类血浆多肽或Fc骨架和其用途
PL2052083T3 (pl) * 2006-09-21 2014-06-30 Scripps Research Inst Genetycznie programowana ekspresja selektywnie siarczanowych białek u eubakterii
US20100055725A1 (en) * 2006-10-17 2010-03-04 Zymera, Inc. System for assays of aminotransferase
WO2008073184A2 (en) 2006-10-18 2008-06-19 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells
GB0623974D0 (en) 2006-11-30 2007-01-10 Medical Res Council Evolved orthogonal ribosomes
KR20140012199A (ko) 2007-03-30 2014-01-29 암브룩스, 인코포레이티드 변형된 ｆｇｆ-21 폴리펩티드 및 그 용도
NZ580686A (en) 2007-05-02 2012-11-30 Ambrx Inc Modified interferon beta polypeptides and their uses
EP2930182A1 (de) 2007-11-20 2015-10-14 Ambrx, Inc. Modifizierte insulinpolypeptide und deren verwendungen
EP3103880A1 (de) 2008-02-08 2016-12-14 Ambrx, Inc. Modifizierte leptinpolypeptide und deren verwendungen
AU2009274076C1 (en) 2008-07-23 2014-04-17 Ambrx, Inc. Modified bovine G-CSF polypeptides and their uses
PL2342223T3 (pl) 2008-09-26 2017-09-29 Ambrx, Inc. Zmodyfikowane polipeptydy zwierzęcej erytropoetyny i ich zastosowania
US9121024B2 (en) 2008-09-26 2015-09-01 Ambrx, Inc. Non-natural amino acid replication-dependent microorganisms and vaccines
WO2011073738A1 (en) 2009-12-14 2011-06-23 Metabolic Explorer Use of inducible promoters in the production of methionine
WO2011087810A1 (en) 2009-12-21 2011-07-21 Ambrx, Inc. Modified porcine somatotropin polypeptides and their uses
BR112012015461A2 (pt) 2009-12-21 2017-01-10 Ambrx Inc polipeptídeos de somatotropina boviina modificados e seus usos
ES2742296T3 (es) 2010-08-17 2020-02-13 Ambrx Inc Polipéptidos de relaxina modificados y sus usos
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
TWI480288B (zh) 2010-09-23 2015-04-11 Lilly Co Eli 牛顆粒細胞群落刺激因子及其變體之調配物
CN102888387B (zh) * 2011-07-21 2015-03-18 中国科学院生物物理研究所 3-氯代酪氨酸翻译系统及其应用
JO3564B1 (ar) 2013-03-08 2020-07-05 Novartis Ag ببتيدات وتركيبات لعلاج ضرر المفاصل
KR20150126035A (ko) 2013-03-13 2015-11-10 일라이 릴리 앤드 캄파니 변형된 개 렙틴 폴리펩티드
WO2015009948A1 (en) 2013-07-19 2015-01-22 Regents Of The University Of California Milk fat globule epidermal growth factor 8 regulates fatty acid uptake
US10011649B2 (en) 2013-08-26 2018-07-03 Arizona Board Of Regents On Behalf Of Arizona State University High affinity synbodies for influenza
WO2016004024A1 (en) 2014-07-02 2016-01-07 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
CN107108710B (zh) 2014-10-24 2022-02-15 百时美施贵宝公司 修饰的fgf-21多肽及其用途
CN107406463A (zh) 2014-12-04 2017-11-28 细胞基因公司 生物分子共轭物
US9744624B2 (en) * 2015-06-17 2017-08-29 Kinsus Interconnect Technology Corp. Method for manufacturing circuit board
WO2018087727A1 (en) 2016-11-14 2018-05-17 Novartis Ag Methods and compositions for treatment of cartilage damage and arthritis
US11339209B2 (en) 2016-11-14 2022-05-24 Novartis Ag Compositions, methods, and therapeutic uses related to fusogenic protein minion
KR102670432B1 (ko) 2017-02-08 2024-05-28 브리스톨-마이어스 스큅 컴퍼니 약동학적 인핸서를 포함하는 변형된 렐락신 폴리펩티드 및 그의 용도
TW202128996A (zh) * 2019-10-10 2021-08-01 美商史基普研究協會 用於活體內合成非天然多肽的組合物及方法
CN120344270A (zh) 2022-10-07 2025-07-18 Ambrx 公司 药物接头及其抗体缀合物
WO2024091824A1 (en) 2022-10-26 2024-05-02 Ada Forsyth Institute, Inc. Differentiation and reprogramming of chondrocyte
CN120882432A (zh) 2023-01-16 2025-10-31 Ambrx 公司 抗cd70抗体-药物缀合物
WO2024178310A1 (en) 2023-02-23 2024-08-29 Ambrx, Inc. Trop2-directed antibody-drug conjugates and uses thereof
WO2024241086A1 (en) 2023-05-24 2024-11-28 Ambrx, Inc. Pegylated bovine interferon lambda and methods of use thereof
WO2025041055A1 (en) 2023-08-22 2025-02-27 Ambrx, Inc. Anti-psma adc conjugate compositions and methods of use thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2219136A1 (en) 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US5756316A (en) 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US5783431A (en) 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
DK2322631T3 (en) * 2001-04-19 2015-01-12 Scripps Research Inst Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA syntetasepar
CA2500653A1 (en) * 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis

Also Published As

Publication number Publication date
CA2502029C (en) 2014-06-10
AU2003277373A1 (en) 2004-05-04
PT1578949E (pt) 2009-07-14
ES2324608T3 (es) 2009-08-11
US20060068478A1 (en) 2006-03-30
US20080227153A1 (en) 2008-09-18
US8114629B2 (en) 2012-02-14
AU2003277373B2 (en) 2009-05-07
US20110318806A1 (en) 2011-12-29
US7910345B2 (en) 2011-03-22
WO2004035743A8 (en) 2005-06-09
DK1578949T3 (da) 2009-07-27
US20090215117A1 (en) 2009-08-27
ATE526414T1 (de) 2011-10-15
HK1083351A1 (en) 2006-06-30
WO2004035743A2 (en) 2004-04-29
DE60327369D1 (de) 2009-06-04
CA2502029A1 (en) 2004-04-29
IL210359A0 (en) 2011-03-31
EP1578949B1 (de) 2009-04-22
AU2009203098B2 (en) 2011-09-08
JP4444113B2 (ja) 2010-03-31
US7432092B2 (en) 2008-10-07
KR20050072441A (ko) 2005-07-11
IL167715A (en) 2010-11-30
EP2062976A2 (de) 2009-05-27
US7524647B2 (en) 2009-04-28
EP2062976B1 (de) 2011-09-28
MXPA05003983A (es) 2005-06-22
EP1578949A2 (de) 2005-09-28
US8071344B2 (en) 2011-12-06
IL201208A (en) 2011-02-28
JP2006507814A (ja) 2006-03-09
US20080176284A1 (en) 2008-07-24
AU2009203098A1 (en) 2009-08-20
WO2004035743A3 (en) 2005-11-10
CY1110483T1 (el) 2015-04-29
EP2062976A3 (de) 2010-04-07
EP1578949A4 (de) 2006-12-20
SI1578949T1 (sl) 2009-08-31

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