ATE393779T1 - Verfahren zur detektion der gamma-sekretase- aktivität - Google Patents
Verfahren zur detektion der gamma-sekretase- aktivitätInfo
- Publication number
- ATE393779T1 ATE393779T1 AT04730223T AT04730223T ATE393779T1 AT E393779 T1 ATE393779 T1 AT E393779T1 AT 04730223 T AT04730223 T AT 04730223T AT 04730223 T AT04730223 T AT 04730223T AT E393779 T1 ATE393779 T1 AT E393779T1
- Authority
- AT
- Austria
- Prior art keywords
- gamma
- secretase
- protein
- cell
- val
- Prior art date
Links
- 102000002659 Amyloid Precursor Protein Secretases Human genes 0.000 title abstract 24
- 108010043324 Amyloid Precursor Protein Secretases Proteins 0.000 title abstract 24
- 230000000694 effects Effects 0.000 title abstract 9
- 108090000623 proteins and genes Proteins 0.000 abstract 22
- 102000004169 proteins and genes Human genes 0.000 abstract 22
- 108020001507 fusion proteins Proteins 0.000 abstract 12
- 102000037865 fusion proteins Human genes 0.000 abstract 12
- 210000004027 cell Anatomy 0.000 abstract 11
- 102000035195 Peptidases Human genes 0.000 abstract 9
- 108091005804 Peptidases Proteins 0.000 abstract 9
- 108700019146 Transgenes Proteins 0.000 abstract 8
- 235000019833 protease Nutrition 0.000 abstract 8
- 239000002773 nucleotide Substances 0.000 abstract 7
- 125000003729 nucleotide group Chemical group 0.000 abstract 7
- 239000002299 complementary DNA Substances 0.000 abstract 5
- 239000000126 substance Substances 0.000 abstract 5
- 230000009261 transgenic effect Effects 0.000 abstract 5
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 3
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 3
- 239000013612 plasmid Substances 0.000 abstract 3
- 239000008194 pharmaceutical composition Substances 0.000 abstract 2
- 230000004568 DNA-binding Effects 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 108700008625 Reporter Genes Proteins 0.000 abstract 1
- 230000027455 binding Effects 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 230000028023 exocytosis Effects 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000010534 mechanism of action Effects 0.000 abstract 1
- 230000000324 neuroprotective effect Effects 0.000 abstract 1
- 230000001777 nootropic effect Effects 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 235000019419 proteases Nutrition 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
- 210000005253 yeast cell Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
- C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Plant Pathology (AREA)
- Neurosurgery (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Neurology (AREA)
- Veterinary Medicine (AREA)
- Hospice & Palliative Care (AREA)
- Psychiatry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20030011807 EP1481987A1 (de) | 2003-05-26 | 2003-05-26 | Screening-Verfahren für Inhibitoren der gamma-Sekretase-Aktivität |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE393779T1 true ATE393779T1 (de) | 2008-05-15 |
Family
ID=33104072
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04730223T ATE393779T1 (de) | 2003-05-26 | 2004-04-29 | Verfahren zur detektion der gamma-sekretase- aktivität |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US7892778B2 (de) |
| EP (2) | EP1481987A1 (de) |
| JP (1) | JP4903046B2 (de) |
| KR (1) | KR101215855B1 (de) |
| CN (1) | CN1795203B (de) |
| AT (1) | ATE393779T1 (de) |
| AU (1) | AU2004241151B2 (de) |
| BR (1) | BRPI0410736A (de) |
| CA (1) | CA2526790C (de) |
| DE (1) | DE602004013440T2 (de) |
| DK (1) | DK1631584T3 (de) |
| ES (1) | ES2307012T3 (de) |
| HK (1) | HK1091215A1 (de) |
| IL (1) | IL172119A (de) |
| MX (1) | MXPA05012663A (de) |
| NO (1) | NO334058B1 (de) |
| RU (1) | RU2373284C2 (de) |
| WO (1) | WO2004104037A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007028655A2 (en) * | 2005-09-09 | 2007-03-15 | H. Lundbeck A/S | Drug discovery for neurodevelopmental disorders and their complications |
| US20090130691A1 (en) * | 2005-09-16 | 2009-05-21 | Institut De Recherches Cliniques De Montreal | Screening proteinase modulators using a chimeric protein and ski-i proprotein convertase substrates and inhibitors |
| KR100883158B1 (ko) * | 2005-10-26 | 2009-02-10 | 주식회사 뉴젝스 | 살아있는 세포에서 단백질분해효소의 활성 측정 방법 |
| JP2007300856A (ja) * | 2006-05-11 | 2007-11-22 | Hiroshi Mori | アミロイドタンパク質模倣物 |
| WO2008061673A2 (en) * | 2006-11-24 | 2008-05-29 | Synthon B.V. | Drug discovery for cancer |
| CN104880441B (zh) * | 2015-05-14 | 2017-12-22 | 上海皓拓生物技术有限公司 | β‑分泌酶特异性抑制剂的筛选方法及其筛选系统 |
| WO2017041037A1 (en) * | 2015-09-04 | 2017-03-09 | The Trustees Of Columbia University In The City Of New York | Disruption of the interaction between amyloid beta peptide and dietary lipids |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998015828A1 (en) * | 1996-10-07 | 1998-04-16 | Scios Inc. | Method to identify direct inhibitors of the beta-amyloid forming enzyme gamma-secretase |
| DE19849073A1 (de) * | 1998-10-24 | 2000-04-27 | Aventis Pharma Gmbh | Transgener C. elegans als Modellorganismus für Untersuchungen zur Alzheimer'schen Krankheit |
| DE19856261C1 (de) * | 1998-12-07 | 2000-03-30 | Hoechst Marion Roussel De Gmbh | Aß-Peptid Screening Assay |
| DE19920514A1 (de) * | 1999-05-05 | 2000-11-16 | Boehringer Ingelheim Pharma | Methoden zur Auffindung von Proteasen, die spezifisch membrangebundene Substrate spalten |
| JP4362035B2 (ja) * | 2001-05-11 | 2009-11-11 | メディカル リサーチ カウンシル | Ｒｈｏｍｂｏｉｄポリペプチドのモジュレーターを同定するアッセイ |
-
2003
- 2003-05-26 EP EP20030011807 patent/EP1481987A1/de not_active Withdrawn
-
2004
- 2004-04-29 DK DK04730223T patent/DK1631584T3/da active
- 2004-04-29 AU AU2004241151A patent/AU2004241151B2/en not_active Ceased
- 2004-04-29 WO PCT/EP2004/004550 patent/WO2004104037A1/en active IP Right Grant
- 2004-04-29 AT AT04730223T patent/ATE393779T1/de not_active IP Right Cessation
- 2004-04-29 RU RU2005140567/13A patent/RU2373284C2/ru not_active IP Right Cessation
- 2004-04-29 EP EP04730223A patent/EP1631584B1/de not_active Expired - Lifetime
- 2004-04-29 BR BRPI0410736-5A patent/BRPI0410736A/pt not_active IP Right Cessation
- 2004-04-29 ES ES04730223T patent/ES2307012T3/es not_active Expired - Lifetime
- 2004-04-29 CN CN2004800142844A patent/CN1795203B/zh not_active Expired - Fee Related
- 2004-04-29 MX MXPA05012663A patent/MXPA05012663A/es active IP Right Grant
- 2004-04-29 JP JP2006529722A patent/JP4903046B2/ja not_active Expired - Fee Related
- 2004-04-29 CA CA2526790A patent/CA2526790C/en not_active Expired - Fee Related
- 2004-04-29 DE DE602004013440T patent/DE602004013440T2/de not_active Expired - Lifetime
- 2004-05-19 US US10/849,423 patent/US7892778B2/en not_active Expired - Fee Related
-
2005
- 2005-11-22 IL IL172119A patent/IL172119A/en not_active IP Right Cessation
- 2005-11-25 KR KR1020057022603A patent/KR101215855B1/ko not_active IP Right Cessation
- 2005-12-19 NO NO20056029A patent/NO334058B1/no not_active IP Right Cessation
-
2006
- 2006-10-25 HK HK06111751.0A patent/HK1091215A1/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CN1795203B (zh) | 2010-06-16 |
| DE602004013440D1 (de) | 2008-06-12 |
| BRPI0410736A (pt) | 2006-06-27 |
| CA2526790C (en) | 2013-12-24 |
| KR20060015312A (ko) | 2006-02-16 |
| EP1481987A1 (de) | 2004-12-01 |
| MXPA05012663A (es) | 2006-05-25 |
| JP2008502302A (ja) | 2008-01-31 |
| CA2526790A1 (en) | 2004-12-02 |
| WO2004104037A1 (en) | 2004-12-02 |
| EP1631584A1 (de) | 2006-03-08 |
| AU2004241151B2 (en) | 2009-04-09 |
| EP1631584B1 (de) | 2008-04-30 |
| RU2373284C2 (ru) | 2009-11-20 |
| HK1091215A1 (en) | 2007-01-12 |
| US20050032150A1 (en) | 2005-02-10 |
| ES2307012T3 (es) | 2008-11-16 |
| JP4903046B2 (ja) | 2012-03-21 |
| KR101215855B1 (ko) | 2012-12-31 |
| NO334058B1 (no) | 2013-12-02 |
| NO20056029L (no) | 2006-02-24 |
| CN1795203A (zh) | 2006-06-28 |
| DE602004013440T2 (de) | 2009-05-20 |
| US7892778B2 (en) | 2011-02-22 |
| RU2005140567A (ru) | 2006-06-27 |
| AU2004241151A1 (en) | 2004-12-02 |
| DK1631584T3 (da) | 2008-09-01 |
| IL172119A (en) | 2010-12-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |