ATE370248T1 - Methode zur quantifizierung von nukleinsäuren in echtzeit mittels effizienzkorrektur - Google Patents
Methode zur quantifizierung von nukleinsäuren in echtzeit mittels effizienzkorrekturInfo
- Publication number
- ATE370248T1 ATE370248T1 AT01107737T AT01107737T ATE370248T1 AT E370248 T1 ATE370248 T1 AT E370248T1 AT 01107737 T AT01107737 T AT 01107737T AT 01107737 T AT01107737 T AT 01107737T AT E370248 T1 ATE370248 T1 AT E370248T1
- Authority
- AT
- Austria
- Prior art keywords
- quantification
- amplification
- nucleic acid
- target nucleic
- real
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 6
- 150000007523 nucleic acids Chemical class 0.000 title abstract 6
- 102000039446 nucleic acids Human genes 0.000 title abstract 6
- 238000011002 quantification Methods 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 2
- 230000003321 amplification Effects 0.000 abstract 5
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 5
- 238000006243 chemical reaction Methods 0.000 abstract 2
- 108700039887 Essential Genes Proteins 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16Z—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS, NOT OTHERWISE PROVIDED FOR
- G16Z99/00—Subject matter not provided for in other main groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP00107036A EP1138780A1 (de) | 2000-03-31 | 2000-03-31 | Detektion ausgesäter Tumorzellen mittels quantitativer RT-PCR auf Cytokeratin-20 mRNA |
| DE10034209 | 2000-07-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE370248T1 true ATE370248T1 (de) | 2007-09-15 |
Family
ID=26006383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01107737T ATE370248T1 (de) | 2000-03-31 | 2001-04-02 | Methode zur quantifizierung von nukleinsäuren in echtzeit mittels effizienzkorrektur |
Country Status (6)
| Country | Link |
|---|---|
| US (4) | US6691041B2 (de) |
| EP (1) | EP1138783B1 (de) |
| JP (1) | JP3589638B2 (de) |
| AT (1) | ATE370248T1 (de) |
| DE (1) | DE60129869T2 (de) |
| ES (1) | ES2291238T3 (de) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10045521A1 (de) * | 2000-03-31 | 2001-10-04 | Roche Diagnostics Gmbh | Nukleinsäureamplifikationen |
| US6691041B2 (en) | 2000-03-31 | 2004-02-10 | Roche Molecular Systems, Inc. | Method for the efficiency-corrected real-time quantification of nucleic acids |
| US7188030B2 (en) | 2001-08-21 | 2007-03-06 | Applera Corporation | Automatic threshold setting for quantitative polymerase chain reaction |
| US7228237B2 (en) | 2002-02-07 | 2007-06-05 | Applera Corporation | Automatic threshold setting and baseline determination for real-time PCR |
| GB0204022D0 (en) * | 2002-02-20 | 2002-04-03 | Onyvax Ltd | Nucleic acid quantification |
| FR2842211A1 (fr) * | 2002-07-09 | 2004-01-16 | Inst Necker | Procede et moyens pour l'analyse quantitative du nombre des molecules d'arnm codant pour des genes differents dans des cellules |
| NL1021160C2 (nl) * | 2002-07-26 | 2004-01-27 | Multigen Internat B V | Werkwijze voor het bepalen van het kopieaantal van een nucleotidevolgorde. |
| EP1418241A1 (de) * | 2002-11-08 | 2004-05-12 | PrimaGen Holding B.V. | Methode zur Quantifizierung des Verhältnisses zweier Nukleinsäuresequenzen |
| ES2294346T3 (es) * | 2002-11-28 | 2008-04-01 | Peter Kufer | Rt-pcr en tiempo real novedosa para la deteccion sensible de multiples transcripciones del gen mage. |
| US7822556B2 (en) | 2003-04-29 | 2010-10-26 | The Jackson Laboratory | Expression data analysis systems and methods |
| US7881873B2 (en) | 2003-04-29 | 2011-02-01 | The Jackson Laboratory | Systems and methods for statistical genomic DNA based analysis and evaluation |
| US7788039B2 (en) | 2003-09-25 | 2010-08-31 | Roche Molecular Systems, Inc. | Quantitation of nucleic acids using growth curves |
| ES2497502T3 (es) | 2003-12-06 | 2014-09-23 | Abbott Laboratories | Procedimiento y sistema para el análisis de las reacciones usando un sistema de información |
| JP4440857B2 (ja) | 2004-09-01 | 2010-03-24 | 三星電子株式会社 | リアルタイム核酸増幅データから初期核酸濃度を定量化する方法 |
| KR100738073B1 (ko) * | 2004-09-01 | 2007-07-12 | 삼성전자주식회사 | 실시간 핵산 증폭 데이터로부터 초기 핵산 농도를정량화하는 방법 |
| JP2006072656A (ja) | 2004-09-01 | 2006-03-16 | Hitachi Software Eng Co Ltd | リアルタイムpcrのプライマー設計方法 |
| US20060178838A1 (en) * | 2005-02-10 | 2006-08-10 | Adelson Martin E | Method of receiving and handling a plurality of clinical samples for reporting a sum of diagnostic results for each sample |
| US20060246423A1 (en) * | 2005-02-10 | 2006-11-02 | Adelson Martin E | Method and kit for the collection and maintenance of the detectability of a plurality of microbiological species in a single gynecological sample |
| US9464310B2 (en) * | 2005-02-10 | 2016-10-11 | Medical Diagnostic Laboratories, Llc | Integrated method for collection and maintenance of detectability of a plurality of microbiological agents in a single clinical sample and for handling a plurality of samples for reporting a sum of diagnostic results for each sample |
| EP2322940B1 (de) | 2005-03-10 | 2014-10-29 | Gen-Probe Incorporated | Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten in Proben |
| EP1930730B1 (de) | 2005-03-10 | 2019-08-14 | Gen-Probe Incorporated | Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten |
| AU2006315489B2 (en) * | 2005-11-14 | 2012-09-06 | Gen-Probe Incorporated | Parametric calibration method |
| US8232091B2 (en) * | 2006-05-17 | 2012-07-31 | California Institute Of Technology | Thermal cycling system |
| DE102006056790B3 (de) * | 2006-12-01 | 2008-01-17 | IfP Privates Institut für Produktqualität GmbH | Laboreinmalartikel für Analyse und Diagnosik |
| WO2009002447A1 (en) | 2007-06-21 | 2008-12-31 | Gen-Probe Incorporated | Instrument and receptacles for use in performing processes |
| US20090018776A1 (en) * | 2007-07-10 | 2009-01-15 | Taylor Roger H | System and method for normalizing data in nucleic acid amplification procedures |
| WO2009012110A2 (en) * | 2007-07-13 | 2009-01-22 | The United States Of America, As Represented By The Secretary Of The Army, On Behalf Of The U.S. Army Medical Research Institute Of Chemical Defense | Unique calibrator polynucleotides and methods of using in quantitative nucleic acid assays |
| US8346485B2 (en) | 2008-11-25 | 2013-01-01 | Quest Diagnostics Investments Incorporated | Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction |
| AU2010208085B2 (en) | 2009-01-30 | 2014-02-06 | Gen-Probe Incorporated | Systems and methods for detecting a signal and applying thermal energy to a signal transmission element |
| DE212010000039U1 (de) * | 2009-04-03 | 2012-02-02 | Helixis, Inc. | Geräte zum erhitzen biologischer proben |
| CN201837588U (zh) * | 2009-09-09 | 2011-05-18 | 海利克斯公司 | 用于多个反应的光学系统 |
| EP2437192A1 (de) * | 2010-09-16 | 2012-04-04 | Roche Diagnostics GmbH | Relative Quantifizierungsanalyse für mehrparametrische PCR-Experimente |
| EP2684022B1 (de) | 2011-03-08 | 2017-09-27 | Universite Laval | Zentripetale fluidikvorrichtung |
| AU2012272763B2 (en) * | 2011-06-24 | 2016-11-03 | Nanostring Technologies, Inc. | Multivariate diagnostic assays and methods for using same |
| JP5955680B2 (ja) * | 2011-09-02 | 2016-07-20 | アークレイ株式会社 | 核酸検出装置、方法、及びプログラム |
| ITMO20120015A1 (it) * | 2012-01-26 | 2013-07-27 | Guescini Michele Proprieta Al 25 | Metodo di perfezionamento per la determinazione quantitativa degli acidi nucleici |
| US20130273547A1 (en) * | 2012-04-16 | 2013-10-17 | Samsung Techwin Co., Ltd. | Method to determine and correct baseline and to characterize pcr amplification kinetics |
| US9932628B2 (en) | 2012-07-27 | 2018-04-03 | Gen-Probe Incorporated | Dual reference calibration method and system for quantifying polynucleotides |
| US10465237B2 (en) * | 2014-01-20 | 2019-11-05 | Life Technologies Corporation | Methods and systems for quantification without standard curves |
| KR102207922B1 (ko) | 2014-03-06 | 2021-01-26 | 삼성전자주식회사 | 반코마이신 저항성 엔테로코커스 특이적인 프라이머 세트, 그를 포함하는 조성물 및 시료 중 반코마이신 저항성 엔테로코커스 속 미생물을 검출하는 방법 |
| US20170177791A1 (en) * | 2014-04-04 | 2017-06-22 | Life Technologies Corporation | Methods and Systems for PCR Quantitation |
| KR102287811B1 (ko) | 2014-10-31 | 2021-08-09 | 삼성전자주식회사 | 2개 표면을 결합시키는 방법 및 그에 의하여 제조된 구조체, 및 상기 구조체를 포함하는 미세유동 장치 |
| EP3020827A1 (de) * | 2014-11-13 | 2016-05-18 | Carpegen GmbH | Verfahren zur Bestimmung einer Eigenschaft einer Ausgangsprobe |
| USD799715S1 (en) | 2015-10-23 | 2017-10-10 | Gene POC, Inc. | Fluidic centripetal device |
| EP3433383B1 (de) | 2016-03-24 | 2022-11-16 | BioFire Diagnostics, LLC | Verfahren zur quantitativen amplifikation |
| EP3486330A1 (de) | 2017-11-21 | 2019-05-22 | Ricoh Company, Ltd. | Vorrichtung |
| GB201720088D0 (en) | 2017-12-01 | 2018-01-17 | Univ Oslo Hf | PCR Controls |
| TWI664292B (zh) * | 2018-02-21 | 2019-07-01 | 奎克生技光電股份有限公司 | 用於低濃度核酸樣品的測量方法 |
| DE102018213026A1 (de) * | 2018-08-03 | 2020-02-06 | Robert Bosch Gmbh | Verfahren zur Durchführung einer Echtzeit-PCR |
| EP3670670A1 (de) * | 2018-12-18 | 2020-06-24 | Ricoh Company, Ltd. | Nukleinsäureanalyseverfahren, nukleinsäureanalyseprogramm und vorrichtung zur bibliotheksherstellung |
| CN110724731A (zh) * | 2019-11-22 | 2020-01-24 | 上海冰缘医疗科技有限公司 | 一种在多重pcr体系内加入内参定量核酸拷贝数的方法 |
| DE102020202358B4 (de) | 2020-02-25 | 2023-09-21 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und Vorrichtung zur Durchführung eines qPCR-Verfahrens |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2516655A (en) * | 1945-08-30 | 1950-07-25 | Maytag Co | Oscillating washing machine tub |
| US3163404A (en) * | 1962-10-09 | 1964-12-29 | Scientific Industries | Rotary apparatus for agitating fluids |
| US3614434A (en) * | 1968-07-17 | 1971-10-19 | Lofstrom James E | Automatic agitating and sample device |
| US3711379A (en) * | 1971-06-24 | 1973-01-16 | Cenco Medical Health Supply Co | Rotating flask culture apparatus |
| GB9020352D0 (en) * | 1990-09-18 | 1990-10-31 | Anagen Ltd | Assay or reaction apparatus |
| CA2129787A1 (en) | 1993-08-27 | 1995-02-28 | Russell G. Higuchi | Monitoring multiple amplification reactions simultaneously and analyzing same |
| US5499872A (en) * | 1994-03-14 | 1996-03-19 | Baxter; Michael | Turntable mixer apparatus |
| AT401270B (de) * | 1994-09-26 | 1996-07-25 | Immuno Ag | Verfahren zur quantifizierung von genomischer dna |
| EP1674585B1 (de) | 1996-06-04 | 2010-10-20 | University of Utah Research Foundation | Vorrichtung zur Durchführung von PCR unter zeitnaher Beobachtung der Fluoreszenz |
| PT912766E (pt) | 1996-06-04 | 2009-07-16 | Univ Utah Res Found | Monitorização da hibridização durante a pcr |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| EP1071824A2 (de) | 1998-04-23 | 2001-01-31 | Genentech, Inc. | Quantitative analyse der genexpression |
| US6066458A (en) | 1998-05-18 | 2000-05-23 | Becton, Dickinson And Company | Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates |
| US6235504B1 (en) * | 1999-01-11 | 2001-05-22 | The Rockefeller University | Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein |
| US6691041B2 (en) * | 2000-03-31 | 2004-02-10 | Roche Molecular Systems, Inc. | Method for the efficiency-corrected real-time quantification of nucleic acids |
| DE10045521A1 (de) | 2000-03-31 | 2001-10-04 | Roche Diagnostics Gmbh | Nukleinsäureamplifikationen |
-
2001
- 2001-03-30 US US09/823,711 patent/US6691041B2/en not_active Expired - Lifetime
- 2001-04-02 AT AT01107737T patent/ATE370248T1/de active
- 2001-04-02 JP JP2001103974A patent/JP3589638B2/ja not_active Expired - Lifetime
- 2001-04-02 ES ES01107737T patent/ES2291238T3/es not_active Expired - Lifetime
- 2001-04-02 DE DE60129869T patent/DE60129869T2/de not_active Expired - Lifetime
- 2001-04-02 EP EP01107737A patent/EP1138783B1/de not_active Expired - Lifetime
-
2003
- 2003-12-24 US US10/746,993 patent/US20040153254A1/en not_active Abandoned
-
2009
- 2009-08-27 US US12/549,150 patent/US8024132B2/en not_active Expired - Fee Related
-
2011
- 2011-06-17 US US13/163,500 patent/US8744777B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DE60129869T2 (de) | 2008-05-08 |
| US6691041B2 (en) | 2004-02-10 |
| JP3589638B2 (ja) | 2004-11-17 |
| US20040153254A1 (en) | 2004-08-05 |
| ES2291238T3 (es) | 2008-03-01 |
| EP1138783A3 (de) | 2003-04-09 |
| DE60129869D1 (de) | 2007-09-27 |
| US20120270222A1 (en) | 2012-10-25 |
| EP1138783A2 (de) | 2001-10-04 |
| JP2001314195A (ja) | 2001-11-13 |
| US8744777B2 (en) | 2014-06-03 |
| US20100021923A1 (en) | 2010-01-28 |
| EP1138783B1 (de) | 2007-08-15 |
| US20030165832A1 (en) | 2003-09-04 |
| US8024132B2 (en) | 2011-09-20 |
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| Date | Code | Title | Description |
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| UEP | Publication of translation of european patent specification |
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