DE60129869D1 - Methode zur Quantifizierung von Nukleinsäuren in Echtzeit mittels Effizienzkorrektur - Google Patents

Methode zur Quantifizierung von Nukleinsäuren in Echtzeit mittels Effizienzkorrektur

Info

Publication number
DE60129869D1
DE60129869D1 DE60129869T DE60129869T DE60129869D1 DE 60129869 D1 DE60129869 D1 DE 60129869D1 DE 60129869 T DE60129869 T DE 60129869T DE 60129869 T DE60129869 T DE 60129869T DE 60129869 D1 DE60129869 D1 DE 60129869D1
Authority
DE
Germany
Prior art keywords
quantification
amplification
nucleic acid
target nucleic
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60129869T
Other languages
English (en)
Other versions
DE60129869T2 (de
Inventor
Karim Dr Tabiti
Gregor Dr Sagner
Richie Dr Soong
Martin Dr Gutekunst
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26006383&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60129869(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from EP00107036A external-priority patent/EP1138780A1/de
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of DE60129869D1 publication Critical patent/DE60129869D1/de
Publication of DE60129869T2 publication Critical patent/DE60129869T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16Z—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS, NOT OTHERWISE PROVIDED FOR
    • G16Z99/00—Subject matter not provided for in other main groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60129869T 2000-03-31 2001-04-02 Methode zur Quantifizierung von Nukleinsäuren in Echtzeit mittels Effizienzkorrektur Expired - Lifetime DE60129869T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP00107036A EP1138780A1 (de) 2000-03-31 2000-03-31 Detektion ausgesäter Tumorzellen mittels quantitativer RT-PCR auf Cytokeratin-20 mRNA
EP00107036 2000-03-31
DE10034209 2000-07-13
DE10034209 2000-07-13

Publications (2)

Publication Number Publication Date
DE60129869D1 true DE60129869D1 (de) 2007-09-27
DE60129869T2 DE60129869T2 (de) 2008-05-08

Family

ID=26006383

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60129869T Expired - Lifetime DE60129869T2 (de) 2000-03-31 2001-04-02 Methode zur Quantifizierung von Nukleinsäuren in Echtzeit mittels Effizienzkorrektur

Country Status (6)

Country Link
US (4) US6691041B2 (de)
EP (1) EP1138783B1 (de)
JP (1) JP3589638B2 (de)
AT (1) ATE370248T1 (de)
DE (1) DE60129869T2 (de)
ES (1) ES2291238T3 (de)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6691041B2 (en) 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10045521A1 (de) * 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
US7188030B2 (en) 2001-08-21 2007-03-06 Applera Corporation Automatic threshold setting for quantitative polymerase chain reaction
US7228237B2 (en) 2002-02-07 2007-06-05 Applera Corporation Automatic threshold setting and baseline determination for real-time PCR
GB0204022D0 (en) * 2002-02-20 2002-04-03 Onyvax Ltd Nucleic acid quantification
FR2842211A1 (fr) * 2002-07-09 2004-01-16 Inst Necker Procede et moyens pour l'analyse quantitative du nombre des molecules d'arnm codant pour des genes differents dans des cellules
NL1021160C2 (nl) * 2002-07-26 2004-01-27 Multigen Internat B V Werkwijze voor het bepalen van het kopieaantal van een nucleotidevolgorde.
EP1418241A1 (de) * 2002-11-08 2004-05-12 PrimaGen Holding B.V. Methode zur Quantifizierung des Verhältnisses zweier Nukleinsäuresequenzen
DE60316464T2 (de) * 2002-11-28 2008-06-26 Kufer, Peter, Dr. Neue real-time rt-pcr methode zur sensitiven detektierung mehrerer mage gen transkripte
US7822556B2 (en) 2003-04-29 2010-10-26 The Jackson Laboratory Expression data analysis systems and methods
US7881873B2 (en) 2003-04-29 2011-02-01 The Jackson Laboratory Systems and methods for statistical genomic DNA based analysis and evaluation
US7788039B2 (en) 2003-09-25 2010-08-31 Roche Molecular Systems, Inc. Quantitation of nucleic acids using growth curves
US7565250B2 (en) * 2003-12-06 2009-07-21 Abbott Laboratories Inc. Method and system for analyzing reactions using an information system
JP4440857B2 (ja) 2004-09-01 2010-03-24 三星電子株式会社 リアルタイム核酸増幅データから初期核酸濃度を定量化する方法
KR100738073B1 (ko) * 2004-09-01 2007-07-12 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 초기 핵산 농도를정량화하는 방법
JP2006072656A (ja) * 2004-09-01 2006-03-16 Hitachi Software Eng Co Ltd リアルタイムｐｃｒのプライマー設計方法
US9464310B2 (en) * 2005-02-10 2016-10-11 Medical Diagnostic Laboratories, Llc Integrated method for collection and maintenance of detectability of a plurality of microbiological agents in a single clinical sample and for handling a plurality of samples for reporting a sum of diagnostic results for each sample
US20060246423A1 (en) * 2005-02-10 2006-11-02 Adelson Martin E Method and kit for the collection and maintenance of the detectability of a plurality of microbiological species in a single gynecological sample
US20060178838A1 (en) * 2005-02-10 2006-08-10 Adelson Martin E Method of receiving and handling a plurality of clinical samples for reporting a sum of diagnostic results for each sample
CA2842404C (en) 2005-03-10 2016-05-10 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
EP2348320B1 (de) 2005-03-10 2024-05-01 Gen-Probe Incorporated Verfahren und Systeme zur Erkennung mehrerer Fluoreszenzemissionssignale
WO2007059179A1 (en) * 2005-11-14 2007-05-24 Gen-Probe Incorporated Parametric calibration method
US8232091B2 (en) * 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
DE102006056790B3 (de) * 2006-12-01 2008-01-17 IfP Privates Institut für Produktqualität GmbH Laboreinmalartikel für Analyse und Diagnosik
US9458451B2 (en) 2007-06-21 2016-10-04 Gen-Probe Incorporated Multi-channel optical measurement instrument
US20090018776A1 (en) * 2007-07-10 2009-01-15 Taylor Roger H System and method for normalizing data in nucleic acid amplification procedures
US8642746B2 (en) * 2007-07-13 2014-02-04 The United States Of America As Represented By The Secretary Of The Army Unique calibrator polynucleotides and methods of using in quantitative nucleic acid assays
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
JP5586631B2 (ja) 2009-01-30 2014-09-10 ジェン−プローブ・インコーポレーテッド 信号を検出し、信号伝送要素に熱エネルギーを加えるためのシステム及び方法
CN202830041U (zh) * 2009-04-03 2013-03-27 Illumina公司 用于加热生物样本的设备
CN201837588U (zh) * 2009-09-09 2011-05-18 海利克斯公司 用于多个反应的光学系统
EP2437192A1 (de) * 2010-09-16 2012-04-04 Roche Diagnostics GmbH Relative Quantifizierungsanalyse für mehrparametrische PCR-Experimente
PT3270141T (pt) 2011-03-08 2020-08-28 Univ Laval Dispositivo centrípeto fluídico
EP2723897A4 (de) * 2011-06-24 2015-03-18 Nanostring Technologies Inc Multivariate diagnostische tests und verfahren zu ihrer verwendung
JP5955680B2 (ja) * 2011-09-02 2016-07-20 アークレイ株式会社 核酸検出装置、方法、及びプログラム
ITMO20120015A1 (it) * 2012-01-26 2013-07-27 Guescini Michele Proprieta Al 25 Metodo di perfezionamento per la determinazione quantitativa degli acidi nucleici
US20130273547A1 (en) * 2012-04-16 2013-10-17 Samsung Techwin Co., Ltd. Method to determine and correct baseline and to characterize pcr amplification kinetics
WO2014018885A2 (en) * 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
SG11201606778TA (en) 2014-01-20 2016-09-29 Life Technologies Corp Methods and systems for quantification without standard curves
KR102207922B1 (ko) 2014-03-06 2021-01-26 삼성전자주식회사 반코마이신 저항성 엔테로코커스 특이적인 프라이머 세트, 그를 포함하는 조성물 및 시료 중 반코마이신 저항성 엔테로코커스 속 미생물을 검출하는 방법
US10385386B2 (en) 2014-04-04 2019-08-20 Life Technologies Corporation Methods and systems for PCR quantitation
KR102287811B1 (ko) 2014-10-31 2021-08-09 삼성전자주식회사 2개 표면을 결합시키는 방법 및 그에 의하여 제조된 구조체, 및 상기 구조체를 포함하는 미세유동 장치
EP3020827A1 (de) * 2014-11-13 2016-05-18 Carpegen GmbH Verfahren zur Bestimmung einer Eigenschaft einer Ausgangsprobe
USD799715S1 (en) 2015-10-23 2017-10-10 Gene POC, Inc. Fluidic centripetal device
CN109963948B (zh) 2016-03-24 2023-01-24 拜奥法尔诊断有限责任公司 用于定量扩增的方法
EP3486330A1 (de) * 2017-11-21 2019-05-22 Ricoh Company, Ltd. Vorrichtung
GB201720088D0 (en) 2017-12-01 2018-01-17 Univ Oslo Hf PCR Controls
TWI664292B (zh) * 2018-02-21 2019-07-01 奎克生技光電股份有限公司 用於低濃度核酸樣品的測量方法
DE102018213026A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Verfahren zur Durchführung einer Echtzeit-PCR
EP3670670A1 (de) * 2018-12-18 2020-06-24 Ricoh Company, Ltd. Nukleinsäureanalyseverfahren, nukleinsäureanalyseprogramm und vorrichtung zur bibliotheksherstellung
CN110724731A (zh) * 2019-11-22 2020-01-24 上海冰缘医疗科技有限公司 一种在多重pcr体系内加入内参定量核酸拷贝数的方法
DE102020202358B4 (de) * 2020-02-25 2023-09-21 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Durchführung eines qPCR-Verfahrens

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2516655A (en) * 1945-08-30 1950-07-25 Maytag Co Oscillating washing machine tub
US3163404A (en) * 1962-10-09 1964-12-29 Scientific Industries Rotary apparatus for agitating fluids
US3614434A (en) * 1968-07-17 1971-10-19 Lofstrom James E Automatic agitating and sample device
US3711379A (en) * 1971-06-24 1973-01-16 Cenco Medical Health Supply Co Rotating flask culture apparatus
GB9020352D0 (en) * 1990-09-18 1990-10-31 Anagen Ltd Assay or reaction apparatus
CA2129787A1 (en) 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
US5499872A (en) * 1994-03-14 1996-03-19 Baxter; Michael Turntable mixer apparatus
AT401270B (de) * 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
PT912766E (pt) * 1996-06-04 2009-07-16 Univ Utah Res Found Monitorização da hibridização durante a pcr
JP4281877B2 (ja) 1996-06-04 2009-06-17 ユニバーシティ オブ ユタ リサーチ ファウンデーション 生物学的プロセスを実行し且つモニタリングするためのシステムと方法
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
AU3867199A (en) 1998-04-23 1999-11-08 Genentech Inc. Quantitative analysis of gene expression
US6066458A (en) 1998-05-18 2000-05-23 Becton, Dickinson And Company Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates
US6235504B1 (en) * 1999-01-11 2001-05-22 The Rockefeller University Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein
US6691041B2 (en) * 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10045521A1 (de) 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen

Also Published As

Publication number Publication date
ES2291238T3 (es) 2008-03-01
JP2001314195A (ja) 2001-11-13
US20040153254A1 (en) 2004-08-05
ATE370248T1 (de) 2007-09-15
US20030165832A1 (en) 2003-09-04
US20120270222A1 (en) 2012-10-25
DE60129869T2 (de) 2008-05-08
EP1138783A2 (de) 2001-10-04
US8744777B2 (en) 2014-06-03
EP1138783A3 (de) 2003-04-09
EP1138783B1 (de) 2007-08-15
US6691041B2 (en) 2004-02-10
JP3589638B2 (ja) 2004-11-17
US8024132B2 (en) 2011-09-20
US20100021923A1 (en) 2010-01-28

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