DE60136121D1 - Methode zur Bestimmung der Effizienz von Nukleinsäureamplifizierungen - Google Patents

Methode zur Bestimmung der Effizienz von Nukleinsäureamplifizierungen

Info

Publication number
DE60136121D1
DE60136121D1 DE60136121T DE60136121T DE60136121D1 DE 60136121 D1 DE60136121 D1 DE 60136121D1 DE 60136121 T DE60136121 T DE 60136121T DE 60136121 T DE60136121 T DE 60136121T DE 60136121 D1 DE60136121 D1 DE 60136121D1
Authority
DE
Germany
Prior art keywords
nucleic acid
determining
efficiency
target nucleic
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60136121T
Other languages
English (en)
Inventor
Karim Dr Tabiti
Gregor Dr Sagner
Richie Dr Soong
Martin Dr Gutekunst
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00107036A external-priority patent/EP1138780A1/de
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of DE60136121D1 publication Critical patent/DE60136121D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60136121T 2000-03-31 2001-04-02 Methode zur Bestimmung der Effizienz von Nukleinsäureamplifizierungen Expired - Lifetime DE60136121D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00107036A EP1138780A1 (de) 2000-03-31 2000-03-31 Detektion ausgesäter Tumorzellen mittels quantitativer RT-PCR auf Cytokeratin-20 mRNA
DE10034209 2000-07-13
DE10045521A DE10045521A1 (de) 2000-03-31 2000-09-13 Nukleinsäureamplifikationen

Publications (1)

Publication Number Publication Date
DE60136121D1 true DE60136121D1 (de) 2008-11-27

Family

ID=27213959

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10045521A Withdrawn DE10045521A1 (de) 2000-03-31 2000-09-13 Nukleinsäureamplifikationen
DE60136121T Expired - Lifetime DE60136121D1 (de) 2000-03-31 2001-04-02 Methode zur Bestimmung der Effizienz von Nukleinsäureamplifizierungen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10045521A Withdrawn DE10045521A1 (de) 2000-03-31 2000-09-13 Nukleinsäureamplifikationen

Country Status (6)

Country Link
US (2) US7125691B2 (de)
EP (1) EP1138784B1 (de)
JP (1) JP4022600B2 (de)
AT (1) ATE411396T1 (de)
DE (2) DE10045521A1 (de)
ES (1) ES2313919T3 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
ATE423622T1 (de) 1998-05-01 2009-03-15 Gen Probe Inc Automatisches isolierungs- und amplifizierungsverfahren für eine zielnukleinsäuresequenz
US6691041B2 (en) 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10045521A1 (de) * 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
AU2002352858A1 (en) * 2001-11-20 2003-06-10 Exact Sciences Corporation Automated sample preparation methods and devices
NL1021160C2 (nl) * 2002-07-26 2004-01-27 Multigen Internat B V Werkwijze voor het bepalen van het kopieaantal van een nucleotidevolgorde.
US7788039B2 (en) * 2003-09-25 2010-08-31 Roche Molecular Systems, Inc. Quantitation of nucleic acids using growth curves
DE602004031152D1 (de) 2003-12-06 2011-03-03 Abbott Lab Verfahren und system zum analysieren von reaktione
JP2006042803A (ja) * 2004-06-28 2006-02-16 Sumitomo Chemical Co Ltd コモンマーモセット由来のシクロフィリンａ遺伝子及びその利用
EP1776482A2 (de) * 2004-06-30 2007-04-25 Applera Corporation Loglineare verstärkung
JP4777631B2 (ja) 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ 核酸増幅分析法および装置
AU2006205055A1 (en) 2005-01-13 2006-07-20 Hsbc North America Holdings Inc. Framework for configuration and release management of group systems software
DE102005006635A1 (de) 2005-01-31 2006-08-10 Osram Opto Semiconductors Gmbh Optisches Element und Verfahren zu dessen Herstellung
EP1930730B1 (de) * 2005-03-10 2019-08-14 Gen-Probe Incorporated Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten
US7964413B2 (en) * 2005-03-10 2011-06-21 Gen-Probe Incorporated Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay
US7831417B2 (en) 2005-11-14 2010-11-09 Gen-Probe Incorporated Parametric calibration method
CA2670258C (en) 2006-11-30 2020-09-08 Gen-Probe Incorporated Quantitative method employing adjustment of pre-defined master calibration curves
KR101506607B1 (ko) * 2007-04-13 2015-03-30 켐제닉스 파마슈티칼스 인크. 세팔로탁신 경구 제형
EP2220227B1 (de) 2007-07-13 2014-09-10 The United States of America, as represented by The Secretary of the Army, on behalf of the U.S. Army Med. Research Institute of Chem. Defense Einmalige kalibrator-polynukleotide und verfahren zur verwendung bei quantitativen nukleinsäure-tests
KR101413659B1 (ko) * 2007-12-06 2014-07-01 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 시료 중의 표적 핵산의초기 농도를 결정하는 방법
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
RU2639515C2 (ru) 2009-04-16 2017-12-21 Конинклейке Филипс Электроникс Н.В. Способы количественного определения нуклеиновых кислот
CA2768391C (en) 2009-07-21 2016-09-06 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
WO2011017567A1 (en) 2009-08-05 2011-02-10 Life Technologies Corporation Methods for the analysis of proximity binding assay data
WO2011130184A2 (en) * 2010-04-11 2011-10-20 Life Technologies Corporation SYSTEMS AND METHODS FOR MODEL-BASED qPCR
EP2752245A3 (de) 2010-07-23 2016-08-24 Beckman Coulter, Inc. System oder Verfahren zur Aufnahme analytischer Einheiten
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
EP2437192A1 (de) 2010-09-16 2012-04-04 Roche Diagnostics GmbH Relative Quantifizierungsanalyse für mehrparametrische PCR-Experimente
EP2641201B1 (de) * 2010-11-16 2018-12-26 Life Technologies Corporation Systeme und verfahren zur analyse von näherungsbindenden testdaten
US8718948B2 (en) 2011-02-24 2014-05-06 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
ES2844324T3 (es) 2011-11-07 2021-07-21 Beckman Coulter Inc Brazo robótico
US9482684B2 (en) 2011-11-07 2016-11-01 Beckman Coulter, Inc. Centrifuge system and workflow
KR20140092377A (ko) 2011-11-07 2014-07-23 베크만 컬터, 인코포레이티드 분취기 시스템 및 작업흐름
US9910054B2 (en) 2011-11-07 2018-03-06 Beckman Coulter, Inc. System and method for processing samples
BR112014011043A2 (pt) 2011-11-07 2017-06-13 Beckman Coulter Inc detecção de recipiente de espécime
JP2014532881A (ja) 2011-11-07 2014-12-08 ベックマン コールター， インコーポレイテッド 標本輸送システムのための磁気制動
ITMO20120015A1 (it) * 2012-01-26 2013-07-27 Guescini Michele Proprieta Al 25 Metodo di perfezionamento per la determinazione quantitativa degli acidi nucleici
WO2013177524A1 (en) * 2012-05-25 2013-11-28 Accugenomics, Inc. Nucleic acid amplification and use thereof
WO2014018885A2 (en) 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
US10993418B2 (en) 2012-08-13 2021-05-04 Life Genetics Lab, Llc Method for measuring tumor burden in patient derived xenograft (PDX) mice
US9957557B2 (en) 2012-08-13 2018-05-01 Life Genetics Lab, Llc Development of a highly sensitive quantification system for assessing DNA degradation and quality in forensic samples
US20140287946A1 (en) * 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
DK2971090T3 (da) 2013-03-15 2020-06-08 Gen Probe Inc Kalibreringsfremgangsmåde, apparat og computerprogramprodukt
EP3765639A4 (de) * 2018-03-16 2021-12-22 Spartan Bioscience Inc. Linear amplifizierte interne kontrolle für nukleinsäureamplifikationsreaktion
CN111816256B (zh) * 2020-01-07 2024-03-29 江苏普瑞悉恩生物科技有限公司 核酸样本检测方法及装置、存储介质及电子设备
DE102020202358B4 (de) 2020-02-25 2023-09-21 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Durchführung eines qPCR-Verfahrens

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2516655A (en) 1945-08-30 1950-07-25 Maytag Co Oscillating washing machine tub
US3163404A (en) 1962-10-09 1964-12-29 Scientific Industries Rotary apparatus for agitating fluids
US3614434A (en) 1968-07-17 1971-10-19 Lofstrom James E Automatic agitating and sample device
US3711379A (en) 1971-06-24 1973-01-16 Cenco Medical Health Supply Co Rotating flask culture apparatus
GB9020352D0 (en) 1990-09-18 1990-10-31 Anagen Ltd Assay or reaction apparatus
CA2129787A1 (en) 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
US5499872A (en) 1994-03-14 1996-03-19 Baxter; Michael Turntable mixer apparatus
AT401270B (de) * 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
DE69735563T2 (de) * 1996-06-04 2007-05-16 University Of Utah Research Foundation, Salt Lake City Vorrichtung und Verfahren zur Durchführung und Überprüfung von biologischen Prozessen
CA2257109C (en) * 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
WO1999054510A2 (en) 1998-04-23 1999-10-28 Genentech, Inc. Quantitative analysis of gene expression
US6066458A (en) 1998-05-18 2000-05-23 Becton, Dickinson And Company Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates
CA2341522A1 (en) 1998-08-27 2000-03-09 Bristol-Myers Squibb Company Novel pharmaceutical salt form
GB9819272D0 (en) 1998-09-03 1998-10-28 Andaris Ltd Microparticles
US6235504B1 (en) * 1999-01-11 2001-05-22 The Rockefeller University Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein
US6691041B2 (en) * 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10045521A1 (de) 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen

Also Published As

Publication number Publication date
JP2001314194A (ja) 2001-11-13
US7125691B2 (en) 2006-10-24
US20050009048A1 (en) 2005-01-13
EP1138784A3 (de) 2003-05-02
EP1138784A2 (de) 2001-10-04
ATE411396T1 (de) 2008-10-15
DE10045521A1 (de) 2001-10-04
EP1138784B1 (de) 2008-10-15
US20020058262A1 (en) 2002-05-16
ES2313919T3 (es) 2009-03-16
JP4022600B2 (ja) 2007-12-19
US7378241B2 (en) 2008-05-27

Similar Documents

Publication Publication Date Title
ATE411396T1 (de) Methode zur bestimmung der effizienz von nukleinsäureamplifizierungen
DK0497784T3 (da) Kvantificering af nukleinsyrer under anvendelse af polymerasekædereaktionen
DE60129869D1 (de) Methode zur Quantifizierung von Nukleinsäuren in Echtzeit mittels Effizienzkorrektur
ATE548464T1 (de) Detektion von nukleinsäuren durch typenspezifische hybrid-capture methode
ES2082217T3 (es) Procedimiento de amplificacion de la señal de emision de un compuesto luminiscente.
DE59509937D1 (de) Verfahren zur Quantifizierung von genomischer DNA
EA200401354A1 (ru) Химерный олигонуклеотидный праймер для способа амплификации нуклеиновой кислоты; продукт для амплификации нуклеиновой кислоты; зонд для способа детекции нуклеиновой кислоты-мишени; материал с иммобилизованной амплифицированной нуклеиновой кислотой и способ его получения; способ определения нуклеотидной последовательности
ATE306561T1 (de) Verfahren zur quantifizierung eines analyten
DE60035459D1 (de) Automatisierte Echtzeit-Analyse von Nukleinsäureamplifikation
ATE394506T1 (de) Verfahren zur bestimmung von nukleinsäuren mittels einer kontrolle
WO2007024840A3 (en) Method of quantitating nucleic acids by flow cytometry microparticle-based array
ATE358733T1 (de) Schnell-verfahren zur detektion von mikroorganismen in lebensmittelproben
WO2002042486A3 (en) Methods for detection of a nucleic acid by sequential amplification
ATE304061T1 (de) Polynukleotid amplifizierungsverfahren
ATE227345T1 (de) Verfahren zur gattungs- und speziesspezifische identifizierung von legionellen
DE60132924D1 (de) Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2)
GB0300802D0 (en) Method for detecting DNA polymerisation
DE602004028993D1 (de) Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion
ES2152995T3 (es) Deteccion de la amplificacion de acido nucleico mediante polarizacion por fluorescencia.
CA2360012A1 (en) Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
WO2002101091A3 (fr) Procede de detection et d'identification de la presence de matieres biologiques provenant de poissons, et oligonucleotides pour sa mise en oeuvre
DK1284296T3 (da) Fremgangsmåde til påvisning af minority genomer i virus-quasispecies under anvendelse af DNA mikrochips
ATE491041T1 (de) Verfahren zur quantifizierung von methylierter dns
ATE308625T1 (de) Detektionsverfahren für escherichia coli
ATE456669T1 (de) Verfahren zur quantifizierung von nukleinsäuren

Legal Events

Date Code Title Description
8364 No opposition during term of opposition