ATE201238T1 - Dns-sequenzierung durch stufenweise ligierung und spaltung - Google Patents

Dns-sequenzierung durch stufenweise ligierung und spaltung

Info

Publication number
ATE201238T1
ATE201238T1 AT95916916T AT95916916T ATE201238T1 AT E201238 T1 ATE201238 T1 AT E201238T1 AT 95916916 T AT95916916 T AT 95916916T AT 95916916 T AT95916916 T AT 95916916T AT E201238 T1 ATE201238 T1 AT E201238T1
Authority
AT
Austria
Prior art keywords
ligation
dna fragments
target polynucleotide
nucleotides
cleavage
Prior art date
Application number
AT95916916T
Other languages
English (en)
Inventor
Sydney Brenner
Original Assignee
Lynx Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lynx Therapeutics Inc filed Critical Lynx Therapeutics Inc
Application granted granted Critical
Publication of ATE201238T1 publication Critical patent/ATE201238T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT95916916T 1994-04-04 1995-03-24 Dns-sequenzierung durch stufenweise ligierung und spaltung ATE201238T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22230094A 1994-04-04 1994-04-04
US08/280,441 US5552278A (en) 1994-04-04 1994-07-25 DNA sequencing by stepwise ligation and cleavage
PCT/US1995/003678 WO1995027080A2 (en) 1994-04-04 1995-03-24 Dna sequencing by stepwise ligation and cleavage

Publications (1)

Publication Number Publication Date
ATE201238T1 true ATE201238T1 (de) 2001-06-15

Family

ID=26916666

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95916916T ATE201238T1 (de) 1994-04-04 1995-03-24 Dns-sequenzierung durch stufenweise ligierung und spaltung

Country Status (12)

Country Link
US (3) US5552278A (de)
EP (1) EP0703991B1 (de)
JP (3) JP3872812B2 (de)
AT (1) ATE201238T1 (de)
AU (1) AU685628B2 (de)
CA (1) CA2163662A1 (de)
DE (1) DE69520917T2 (de)
DK (1) DK0703991T3 (de)
ES (1) ES2159635T3 (de)
GR (1) GR3036314T3 (de)
PT (1) PT703991E (de)
WO (1) WO1995027080A2 (de)

Families Citing this family (350)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69332879T2 (de) * 1992-03-13 2004-02-19 Diatide, Inc. Technetium-99-markierte peptide zur visualisierung vom entzuendungen
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5695934A (en) * 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US6974666B1 (en) * 1994-10-21 2005-12-13 Appymetric, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US5866330A (en) 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5695937A (en) * 1995-09-12 1997-12-09 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US5763175A (en) * 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5851772A (en) * 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
WO1997046704A1 (en) * 1996-06-06 1997-12-11 Lynx Therapeutics, Inc. Sequencing by ligation of encoded adaptors
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
US6258533B1 (en) 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
IL156002A0 (en) * 1997-02-12 2003-12-23 Eugene Y Chan Methods and products for analyzing polymers
IL120339A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US5994068A (en) 1997-03-11 1999-11-30 Wisconsin Alumni Research Foundation Nucleic acid indexing
EP2327797B1 (de) 1997-04-01 2015-11-25 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
US5888737A (en) * 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
JP4294740B2 (ja) * 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド 分析物の系列的プロセシングのためのシステムおよび装置
AU739963B2 (en) 1997-06-27 2001-10-25 Lynx Therapeutics, Inc. Method of mapping restriction sites in polynucleotides
JP4789294B2 (ja) * 1997-08-22 2011-10-12 ソレクサ・インコーポレイテッド ローリングプライマーを用いたｄｎａ伸長および分析
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
GB9803930D0 (en) * 1998-02-26 1998-04-22 Univ Glasgow Polynucleotide sequencing method and kits therefor
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
DE19822287C2 (de) * 1998-05-18 2003-04-24 Switch Biotech Ag Klonierungsvektor, seine Herstellung und Verwendung zur Analyse von mRNA Expressionsmuster
US7060431B2 (en) 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
US6162602A (en) * 1998-07-16 2000-12-19 Gautsch; James W. Automatic direct sequencing of bases in nucleic acid chain elongation
US7270958B2 (en) * 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6500650B1 (en) 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
US6855500B2 (en) 1998-10-01 2005-02-15 Sequenom, Inc. Fluorescence-based genotyping
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6566059B1 (en) 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
WO2000036152A1 (en) * 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
EP1147220A1 (de) * 1999-01-29 2001-10-24 Keygene N.V. Verfahren und zusammensetzung zur bestimmung von einem oder mehreren restriktionsenzymen zur analyse von nukleinsäuren oder nukleinsäuresequenzen
JP2002537856A (ja) * 1999-03-05 2002-11-12 トランジェーヌ ヌクレオチド断片、プローブ、プライマー、試薬、及びｐＢＲ３２２の複製起点由来のヌクレオチド配列を検出する方法
US6521428B1 (en) * 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
AU7569600A (en) 1999-05-20 2000-12-28 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US20020119448A1 (en) * 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
ATE318932T1 (de) * 1999-08-13 2006-03-15 Univ Yale Binär kodierte sequenzmarker
GB0016472D0 (en) * 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
GB0016473D0 (en) * 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
US20060057565A1 (en) * 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
EP3034627B1 (de) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massives paralleles verfahren zum entschlüsseln von dna und rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
US6949340B2 (en) * 2001-03-28 2005-09-27 Creative Mines Llc Optical phase modulator
EP1262563A3 (de) * 2001-05-31 2003-02-05 Takara Bio Inc. Methode zur Fixierung von DNA auf Oberflächen
US6613523B2 (en) * 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
EP1472335A4 (de) * 2001-11-08 2005-12-28 Univ Johns Hopkins Verfahren und systeme zur nukleinsäuresequenzierung
US20030170700A1 (en) * 2002-01-09 2003-09-11 Lynx Therapeutics, Inc. Secreted and cell surface polypeptides affected by cholesterol and uses thereof
US20030166026A1 (en) * 2002-01-09 2003-09-04 Lynx Therapeutics, Inc. Identification of specific biomarkers for breast cancer cells
US20030180764A1 (en) * 2002-01-09 2003-09-25 Lynx Therapeutics, Inc. Genes affected by cholesterol treatment and during adipogenesis
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
US7291460B2 (en) * 2002-05-31 2007-11-06 Verenium Corporation Multiplexed systems for nucleic acid sequencing
US7074597B2 (en) * 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
US20040166567A1 (en) * 2002-09-26 2004-08-26 Santi Daniel V Synthetic genes
WO2004055160A2 (en) * 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
US20040224330A1 (en) * 2003-01-15 2004-11-11 Liyan He Nucleic acid indexing
WO2005010184A1 (ja) * 2003-07-25 2005-02-03 Takara Bio Inc. 変異の検出方法
WO2005017186A2 (en) * 2003-08-08 2005-02-24 Thomas Jefferson University Method for rapid identification of alternative splicing
US20050094807A1 (en) * 2003-11-04 2005-05-05 John Silzel Accuracy array assay system and method
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
JP2007521833A (ja) * 2004-02-12 2007-08-09 コンパス ジェネティクス エルエルシー 配列特異的分類による遺伝的分析
US7622279B2 (en) * 2004-03-03 2009-11-24 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
US20050227316A1 (en) * 2004-04-07 2005-10-13 Kosan Biosciences, Inc. Synthetic genes
WO2006073436A2 (en) * 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
GB0422551D0 (en) * 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
EP2857523A1 (de) 2005-02-01 2015-04-08 Applied Biosystems, LLC Verfahren zur Sequenzbestimmung in einem Polynucleotid
EP2236628A3 (de) * 2005-02-01 2010-10-13 AB Advanced Genetic Analysis Corporation Reagentien, Verfahren und Bibliotheken zur Sequenzierung mit Kügelchen
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
WO2006099604A2 (en) * 2005-03-16 2006-09-21 Compass Genetics, Llc Methods and compositions for assay readouts on multiple analytical platforms
WO2007002204A2 (en) 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070065834A1 (en) * 2005-09-19 2007-03-22 Hillis William D Method and sequences for determinate nucleic acid hybridization
WO2007062105A2 (en) * 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
JPWO2007097443A1 (ja) * 2006-02-20 2009-07-16 国立大学法人 北海道大学 Ｄｎａ塩基配列を決定する方法
AU2007237909A1 (en) * 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
US20090002703A1 (en) * 2006-08-16 2009-01-01 Craig Edward Parman Methods and systems for quantifying isobaric labels and peptides
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7947446B2 (en) * 2007-05-29 2011-05-24 Ming-Sheng Lee High throughput mutation screening methods and kits using a universalized approach—differential sequence fill-in (DSF)-enabled sequential adapter ligation and amplification
EP2201021A4 (de) 2007-08-29 2012-01-25 Applied Biosystems Llc Alternative nukleinsäuresequenzierverfahren
EP2209911B1 (de) 2007-10-19 2013-10-16 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit reversiblen nicht-fluoreszenz-nukleotid-terminatoren und mit einer spaltbaren markierung modifizierten nukleotidterminatoren und eine deoxyinosineverbindung mit einer reversiblen terminatorgruppe
EP2207900B1 (de) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Konstruktion und synthese spaltbarer fluoreszenznukleotide als reversible terminatoren für die dna-sequenzierung mittels synthese
US8101353B2 (en) * 2007-12-18 2012-01-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
WO2009086353A1 (en) * 2007-12-26 2009-07-09 Helicos Biosciences Corporation Improved two-primer sequencing for high-throughput expression analysis
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
US20110105356A1 (en) * 2008-05-07 2011-05-05 Derosier Chad F Compositions and methods for providing substances to and from an array
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
EP2373817A4 (de) * 2008-12-10 2013-01-02 Illumina Inc Verfahren und zusammensetzungen zur hybridisierung von nukleinsäuren
GB0904934D0 (en) 2009-03-23 2009-05-06 Geneseqe As Method and apparatus for detecting molecules
US20110070590A1 (en) 2009-09-22 2011-03-24 Jan Rohozinski Primers and Methods for Determining RhD Zygosity
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
KR101866401B1 (ko) 2010-04-05 2018-06-11 프로그노시스 바이오사이언스, 인코포레이티드 공간적으로 엔코딩된 생물학적 검정
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10240194B2 (en) 2010-05-13 2019-03-26 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US8483969B2 (en) 2010-09-17 2013-07-09 Illuminia, Inc. Variation analysis for multiple templates on a solid support
WO2012050920A1 (en) 2010-09-29 2012-04-19 Illumina, Inc. Compositions and methods for sequencing nucleic acids
GB201016484D0 (en) 2010-09-30 2010-11-17 Geneseque As Method
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
CA2856163C (en) 2011-10-28 2019-05-07 Illumina, Inc. Microarray fabrication system and method
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
GB201215435D0 (en) * 2012-08-30 2012-10-17 Geneseque As Sequencing method
EP3901280B1 (de) 2012-10-17 2025-03-12 10x Genomics Sweden AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP2970951B1 (de) 2013-03-13 2019-02-20 Illumina, Inc. Verfahren zur nukleinsäuresequenzierung
CA2898453C (en) 2013-03-13 2021-07-27 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
ES2685549T3 (es) 2013-03-15 2018-10-09 Illumina Cambridge Limited Nucleósidos o nucleótidos modificados
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
DK3431614T3 (da) 2013-07-01 2021-12-06 Illumina Inc Katalysator fri overfladefunktionalisering og polymerpodning
CN105358715B (zh) 2013-07-03 2018-09-18 伊鲁米那股份有限公司 正交合成测序
EP3038834B1 (de) 2013-08-30 2018-12-12 Illumina, Inc. Manipulation von tröpfchen auf hydrophilen oder variegiert hydrophilen oberflächen
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
BR112016014923B1 (pt) 2013-12-23 2022-11-16 Illumina, Inc Arranjo, método para produção de um arranjo e método para detecção de ácidos nucleicos
US10934581B2 (en) 2014-04-30 2021-03-02 International Business Machines Corporation Bow tie DNA compositions and methods
SG11201610357UA (en) 2014-06-13 2017-01-27 Illumina Cambridge Ltd Methods and compositions for preparing sequencing libraries
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
US10605766B2 (en) 2014-07-15 2020-03-31 Illumina, Inc. Biochemically activated electronic device
GB201413929D0 (en) 2014-08-06 2014-09-17 Geneseque As Method
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
EP3183577B1 (de) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible oberflächenfunktionalisierung
BR122021026779B1 (pt) 2014-10-17 2023-12-19 Illumina Cambridge Limited Transposon de preservação de contiguidade
PT3212684T (pt) 2014-10-31 2020-02-03 Illumina Cambridge Ltd Revestimentos de polímeros e de copolímero de dna
EP4321627A3 (de) 2015-04-10 2024-04-17 10x Genomics Sweden AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
EP3283870B1 (de) 2015-04-14 2020-05-06 Illumina, Inc. Strukturierte substrate zur verbesserten detektion von lichtemissionen und verfahren welche damit zusammenhängen
CN107810415B (zh) 2015-05-11 2021-06-22 亿明达股份有限公司 用于发现和分析治疗剂的平台
HK1253950A1 (zh) 2015-07-07 2019-07-05 Illumina, Inc. 经由纳米压印的选择性表面图案化
EP3325648B1 (de) 2015-07-17 2023-03-29 Illumina, Inc. Polymerfolien zur sequenzierung von anwendungen
IL294145B2 (en) 2015-07-30 2024-02-01 Illumina Inc Removal of orthogonal blocking of nucleotides
WO2017037078A1 (en) 2015-09-02 2017-03-09 Illumina Cambridge Limited Systems and methods of improving droplet operations in fluidic systems
CA3168463A1 (en) 2015-12-17 2017-06-22 Illumina, Inc. Distinguishing methylation levels in complex biological samples
US10907195B1 (en) * 2016-02-16 2021-02-02 Pharmaseq, Inc. Devices and methods for assembling extremely long DNA molecules
SG11201805665UA (en) 2016-03-28 2018-10-30 Illumina Inc Multi-plane microarrays
WO2017177017A1 (en) 2016-04-07 2017-10-12 Omniome, Inc. Methods of quantifying target nucleic acids and identifying sequence variants
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CA3050241C (en) 2017-01-20 2022-11-29 Omniome, Inc. Allele-specific capture of nucleic acids
CN110177884B (zh) 2017-01-20 2021-08-06 欧姆尼欧美公司 通过聚合酶结合进行的基因分型
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
JP2021500531A (ja) 2017-08-15 2021-01-07 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 化学的および生物学的検体の検出に有用なスキャン装置および方法
KR20200059243A (ko) 2017-09-20 2020-05-28 리제너론 파마슈티칼스 인코포레이티드 패신저 유전자 돌연변이 부담이 높은 종양을 가진 환자에 대한 면역 치료요법
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
NL2021377B1 (en) 2018-07-03 2020-01-08 Illumina Inc Interposer with first and second adhesive layers
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020047005A1 (en) 2018-08-28 2020-03-05 10X Genomics, Inc. Resolving spatial arrays
CN113767175B (zh) 2018-08-28 2025-07-25 10X基因组学股份有限公司 增加空间阵列分辨率
JP7528067B2 (ja) 2018-10-25 2024-08-05 イルミナ インコーポレイテッド インデックス及びバーコードを使用してアレイ上のリガンドを識別するための方法及び組成物
CN211311438U (zh) 2018-12-04 2020-08-21 欧姆尼欧美公司 流动池
AU2019391274B2 (en) 2018-12-05 2025-10-30 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
EP3894585A2 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Erzeugung von erfassungssonden zur räumlichen analyse
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
CN112739830A (zh) 2018-12-18 2021-04-30 伊卢米纳剑桥有限公司 使用单一表面引物进行配对末端测序的方法和组合物
BR112020026667A2 (pt) 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
EP3899032A2 (de) 2018-12-20 2021-10-27 Omniome, Inc. Temperaturkontrolle zur analyse von nukleinsäuren und anderen analyten
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
EP3931354A1 (de) 2019-02-28 2022-01-05 10X Genomics, Inc. Profilierung von biologischen analyten mit räumlich barcodierten oligonukleotid-arrays
EP3938538A1 (de) 2019-03-15 2022-01-19 10X Genomics, Inc. Verfahren zur verwendung von räumlichen anordnungen für einzelzellsequenzierung
EP3887542A1 (de) 2019-03-22 2021-10-06 10X Genomics, Inc. Dreidimensionale räumliche analyse
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
EP4028407B1 (de) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible nukleotide änderung
JP7796538B2 (ja) 2019-09-20 2026-01-09 イルミナ インコーポレイテッド インデックス及びバーコードを使用してアレイ上のリガンドを識別するための方法及び組成物
US11514575B2 (en) 2019-10-01 2022-11-29 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US12154266B2 (en) 2019-11-18 2024-11-26 10X Genomics, Inc. Systems and methods for binary tissue classification
EP4062373B1 (de) 2019-11-21 2025-01-22 10X Genomics, Inc. Räumliche analyse von analyten
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
EP4752521A3 (de) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixierreagenzien und verfahren zur verwendung davon
AU2020412459B2 (en) 2019-12-23 2022-12-08 Singular Genomics Systems, Inc. Methods for long read sequencing
EP4081656A1 (de) 2019-12-23 2022-11-02 10X Genomics, Inc. Zusammensetzungen und verfahren zur verwendung von fixierten biologischen proben bei partitionsbasierten tests
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
WO2021158511A1 (en) 2020-02-04 2021-08-12 Omniome, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
EP4104179B1 (de) 2020-02-13 2026-01-28 10X Genomics, Inc. Systeme und methoden zur gemeinsamen interaktiven visualisierung der genexpression und der zugänglichkeit von dna-chromatin
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
WO2021167986A1 (en) 2020-02-17 2021-08-26 10X Genomics, Inc. In situ analysis of chromatin interaction
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
EP4107285B1 (de) 2020-02-21 2024-10-09 10X Genomics, Inc. Verfahren und zusammensetzungen für integrierten räumlichen in-situ-test
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
EP4114966B1 (de) 2020-03-03 2025-02-26 Pacific Biosciences Of California, Inc. Verfahren und zusammensetzungen zur sequenzierung von doppelsträngigen nukleinsäuren
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
WO2021178893A2 (en) 2020-03-06 2021-09-10 Singular Genomics Systems, Inc. Linked paired strand sequencing
EP4139485B1 (de) 2020-04-22 2023-09-06 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
WO2021231263A2 (en) 2020-05-12 2021-11-18 Singular Genomics Systems, Inc. Nucleic acid amplification methods
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775B1 (de) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4600376A3 (de) 2020-06-02 2025-10-22 10X Genomics, Inc. Räumliche transkriptomik für antigenrezeptoren
EP4025692A2 (de) 2020-06-02 2022-07-13 10X Genomics, Inc. Nukleinsäure-bibliotheksverfahren
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252375A1 (en) 2020-06-08 2021-12-16 The Broad Institute, Inc. Single cell combinatorial indexing from amplified nucleic acids
EP4421186B1 (de) 2020-06-08 2025-08-13 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
EP4446430A3 (de) 2020-06-10 2024-12-18 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
EP4164796A4 (de) 2020-06-10 2024-03-06 10x Genomics, Inc. Flüssigkeitsabgabeverfahren
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
EP4450639B1 (de) 2020-06-25 2025-10-15 10X Genomics, Inc. Räumliche analyse der dna-methylierung
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
EP4153606A4 (de) 2020-07-13 2024-10-02 Singular Genomics Systems, Inc. Verfahren zur sequenzierung komplementärer polynukleotide
US11492662B2 (en) 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US12580044B1 (en) 2020-09-02 2026-03-17 10X Genomics, Inc. Systems and methods for identifying cells that are antigen-specific for an immunogenic feature
CN116194590A (zh) 2020-09-18 2023-05-30 10X基因组学有限公司 样品处理设备和图像配准方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
EP4208470A4 (de) 2020-10-22 2024-10-30 Singular Genomics Systems, Inc. Nukleinsäurezirkularisierung und amplifikation auf einer oberfläche
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (de) 2020-12-11 2022-06-15 10X Genomics, Inc. Verfahren und zusammensetzungen zur multimodalen in-situ-analyse
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284945B1 (de) 2021-01-26 2024-10-23 10X Genomics, Inc. Nukleinsäureanalogsonden zur in-situ-analyse
WO2022170212A1 (en) 2021-02-08 2022-08-11 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
US20240043915A1 (en) 2021-02-13 2024-02-08 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
ES2989523T3 (es) 2021-02-19 2024-11-26 10X Genomics Inc Método de uso de un dispositivo modular de soporte para ensayos
WO2022187366A1 (en) 2021-03-03 2022-09-09 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022192671A1 (en) 2021-03-12 2022-09-15 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
US11884977B2 (en) 2021-03-12 2024-01-30 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
EP4524261B1 (de) 2021-03-18 2026-07-22 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
EP4305196B1 (de) 2021-04-14 2025-04-02 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
WO2022232308A1 (en) 2021-04-27 2022-11-03 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
WO2022235764A1 (en) 2021-05-05 2022-11-10 Singular Genomics Systems, Inc. Multiomic analysis device and methods of use thereof
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
CN117396613A (zh) 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
US20230084407A1 (en) 2021-06-02 2023-03-16 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4779305A2 (de) 2021-07-13 2026-07-22 10X Genomics, Inc. Verfahren zur herstellung einer polymerisierten matrix mit steuerbarer dicke
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
EP4326900B1 (de) 2021-07-30 2024-09-04 10X Genomics, Inc. Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
EP4381095A1 (de) 2021-08-03 2024-06-12 10X Genomics, Inc. Nukleinsäurekoncatamere und verfahren zur stabilisierung und/oder verdichtung davon
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12435364B2 (en) 2021-08-16 2025-10-07 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
EP4388134A4 (de) 2021-08-20 2025-10-29 Singular Genomics Systems Inc Chemische und thermische unterstützte nukleinsäureamplifikationsverfahren
EP4344443A1 (de) 2021-08-31 2024-04-03 Illumina, Inc. Durchflusszelle mit verbesserter bohrlochabbildungsauflösung
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023076832A1 (en) 2021-10-25 2023-05-04 Singular Genomics Systems, Inc. Manipulating and detecting biological samples
WO2023076833A1 (en) 2021-10-26 2023-05-04 Singular Genomics Systems, Inc. Multiplexed targeted amplification of polynucleotides
EP4419707A1 (de) 2021-11-10 2024-08-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position eines analyten in einer biologischen probe
EP4305195A2 (de) 2021-12-01 2024-01-17 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme für verbesserten in-situ-nachweis von analyten und raumanalyse
EP4441711A1 (de) 2021-12-20 2024-10-09 10X Genomics, Inc. Selbsttest für eine pathologie-/histologie-objektträgerbildgebungsvorrichtung
US12668834B2 (en) 2021-12-22 2026-06-30 Singular Genomics Systems, Inc. Strand displacing amplification enzymes
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
AU2022424380A1 (en) 2021-12-29 2024-01-18 Illumina, Inc. Methods of nucleic acid sequencing using surface-bound primers
US12674196B2 (en) 2022-01-18 2026-07-07 10X Genomics, Inc. Methods and compositions for improved probe specificity
EP4733410A3 (de) 2022-01-21 2026-05-27 10X Genomics, Inc. Mehrere auslesesignale zur analyse einer probe
US12637489B2 (en) 2022-02-02 2026-05-26 Singular Genomics Systems, Inc. Silicon containing modified nucleotide analogs
US20250188521A1 (en) 2022-03-10 2025-06-12 Singular Genomics Systems, Inc. Nucleic acid delivery scaffolds
US12637706B2 (en) 2022-03-23 2026-05-26 10X Genomics, Inc. Methods and compositions related to microsphere surface gelation
US12703879B2 (en) 2022-03-29 2026-08-11 10X Genomics, Inc. RNase H assisted in situ rolling circle amplification
CN119032271A (zh) 2022-04-01 2024-11-26 10X基因组学有限公司 用于靶向掩蔽自发荧光的组合物和方法
WO2023196526A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods for multiplex cell analysis
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
US12692484B2 (en) 2022-08-12 2026-07-28 10X Genomics, Inc. PUMA1 polymerases and uses thereof
US12116626B2 (en) 2022-08-16 2024-10-15 10X Genomics, Inc. AP50 polymerases and uses thereof
US20240191297A1 (en) 2022-10-14 2024-06-13 10X Genomics, Inc. Methods, compositions, and systems for assessing biological sample quality
WO2024102736A1 (en) 2022-11-08 2024-05-16 10X Genomics, Inc. Immobilization methods and compositions for in situ detection
EP4685239A1 (de) 2022-11-09 2026-01-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position mehrerer analyten in einer biologischen probe
US20240158852A1 (en) 2022-11-16 2024-05-16 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
WO2024107727A1 (en) 2022-11-18 2024-05-23 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
EP4622746A1 (de) 2022-11-22 2025-10-01 10X Genomics, Inc. Systeme und verfahren zur schnittstellenbildung mit einer flasche
US12644837B2 (en) 2022-11-22 2026-06-02 10X Genomics, Inc. Systems and methods for imaging samples
US12581050B2 (en) 2022-11-23 2026-03-17 10X Genomics, Inc. Optical assemblies for machine vision calibration
WO2024129672A1 (en) 2022-12-12 2024-06-20 The Broad Institute, Inc. Trafficked rnas for assessment of cell-cell connectivity and neuroanatomy
US20240209419A1 (en) 2022-12-14 2024-06-27 Illumina, Inc. Systems and Methods for Capture and Enrichment of Clustered Beads on Flow Cell Substrates
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
EP4638789A1 (de) 2022-12-21 2025-10-29 10X Genomics, Inc. Verfahren zur bindung von ribonukleinsäuren in biologischen proben
WO2024229260A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
EP4461816A1 (de) 2023-05-10 2024-11-13 10X Genomics, Inc. Boronsäurezusammensetzungen und verfahren zur bindung von ribonukleinsäuren in biologischen proben
US20240401129A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Methods for double-stranded sequencing by synthesis
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
US20250163502A1 (en) 2023-11-22 2025-05-22 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
EP4578960A1 (de) 2023-12-20 2025-07-02 10x Genomics, Inc. In-situ-detektion von kopienzahlvariationen in biologischen proben
US20250288988A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
WO2025207535A1 (en) 2024-03-26 2025-10-02 Illumina, Inc. Photo-switchable surfaces
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
WO2025264702A1 (en) 2024-06-18 2025-12-26 10X Genomics, Inc. 3d hydrogels for nucleic acid sequencing
WO2025264836A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2025264831A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2026006314A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Tagging target regions prior to nucleotide sequencing
WO2026043885A1 (en) 2024-08-20 2026-02-26 10X Genomics, Inc. Methods and systems for preparing a nucleic acid library
WO2026090042A1 (en) 2024-10-21 2026-04-30 The Broad Institute, Inc. Single-cell and spatial dna methylation measurements
WO2026087220A1 (en) 2024-10-22 2026-04-30 F. Hoffmann-La Roche Ag Preparation of dna libraries for next generation sequencing using y-adapters modified with phosphorothioate bonds
WO2026093331A1 (en) 2024-10-28 2026-05-07 F. Hoffmann-La Roche Ag Hairpin adapter constructs and primers to generate next-generation sequencing library molecules
WO2026099278A1 (en) 2024-11-08 2026-05-15 F. Hoffmann-La Roche Ag Normalizing and pooling of pcr-free sequencing libraries
WO2026128365A1 (en) 2024-12-09 2026-06-18 Illumina, Inc. Methods for increasing sequencing quality
WO2026136945A1 (en) 2024-12-20 2026-06-25 10X Genomics, Inc. Methods and compositions for selective control of signal generation efficiency

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
US4321365A (en) * 1977-10-19 1982-03-23 Research Corporation Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
US4293652A (en) * 1979-05-25 1981-10-06 Cetus Corporation Method for synthesizing DNA sequentially
GB2258950B (en) * 1982-05-11 1993-07-07 Secr Defence Integrated antenna device with resistive connection
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5242794A (en) * 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0224126A3 (de) * 1985-11-25 1989-02-01 The University of Calgary Kovalent gekoppelte komplementäre Oligodeoxynukleotide zur Verwendung als "Primer Linkers" bei Sequenzierung von Nukleinsäuren
GB8612087D0 (en) * 1986-05-19 1986-06-25 Ici Plc Hybridisation probes
US5202231A (en) * 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US5102785A (en) * 1987-09-28 1992-04-07 E. I. Du Pont De Nemours And Company Method of gene mapping
US5093245A (en) * 1988-01-26 1992-03-03 Applied Biosystems Labeling by simultaneous ligation and restriction
US5002867A (en) * 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
DE58908694D1 (de) * 1988-05-24 1995-01-12 Biotechnolog Forschung Gmbh Oligonucleotidbank und verfahren zur dna-sequenzierung.
US5489507A (en) * 1988-11-30 1996-02-06 Perkin-Elmer Corporation DNA detection by color complementation
EP0392546A3 (de) * 1989-04-14 1991-09-11 Ro Institut Za Molekularnu Genetiku I Geneticko Inzenjerstvo Verfahren zur Bestimmung von vollständigen oder partiellen Bestandteilen von sehr kurzen Sequenzen in Proben von Partikeln mikroskopischer Grösse mittels Hybridisierung mit Oligonukleotid-Sonden
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5427911A (en) * 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
DE4321198A1 (de) * 1993-06-25 1995-01-05 Henkel Kgaa Verwendung von N-Allylaminoaromaten in Oxidationsfärbemitteln
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids

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WO1995027080A3 (en) 1995-11-16
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DE69520917D1 (de) 2001-06-21
AU2379195A (en) 1995-10-23
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PT703991E (pt) 2001-10-30
US5552278A (en) 1996-09-03
US5856093A (en) 1999-01-05
ES2159635T3 (es) 2001-10-16
DK0703991T3 (da) 2001-06-18
EP0703991A1 (de) 1996-04-03
JP2005323607A (ja) 2005-11-24
EP0703991B1 (de) 2001-05-16
WO1995027080A2 (en) 1995-10-12
AU685628B2 (en) 1998-01-22
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DE69520917T2 (de) 2001-12-20
GR3036314T3 (en) 2001-10-31
US5599675A (en) 1997-02-04

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