ATE201238T1 - Dns-sequenzierung durch stufenweise ligierung und spaltung - Google Patents

Dns-sequenzierung durch stufenweise ligierung und spaltung

Info

Publication number
ATE201238T1
ATE201238T1 AT95916916T AT95916916T ATE201238T1 AT E201238 T1 ATE201238 T1 AT E201238T1 AT 95916916 T AT95916916 T AT 95916916T AT 95916916 T AT95916916 T AT 95916916T AT E201238 T1 ATE201238 T1 AT E201238T1
Authority
AT
Austria
Prior art keywords
ligation
dna fragments
target polynucleotide
nucleotides
cleavage
Prior art date
Application number
AT95916916T
Other languages
English (en)
Inventor
Sydney Brenner
Original Assignee
Lynx Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lynx Therapeutics Inc filed Critical Lynx Therapeutics Inc
Application granted granted Critical
Publication of ATE201238T1 publication Critical patent/ATE201238T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT95916916T 1994-04-04 1995-03-24 Dns-sequenzierung durch stufenweise ligierung und spaltung ATE201238T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22230094A 1994-04-04 1994-04-04
US08/280,441 US5552278A (en) 1994-04-04 1994-07-25 DNA sequencing by stepwise ligation and cleavage
PCT/US1995/003678 WO1995027080A2 (en) 1994-04-04 1995-03-24 Dna sequencing by stepwise ligation and cleavage

Publications (1)

Publication Number Publication Date
ATE201238T1 true ATE201238T1 (de) 2001-06-15

Family

ID=26916666

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95916916T ATE201238T1 (de) 1994-04-04 1995-03-24 Dns-sequenzierung durch stufenweise ligierung und spaltung

Country Status (12)

Country Link
US (3) US5552278A (de)
EP (1) EP0703991B1 (de)
JP (3) JP3872812B2 (de)
AT (1) ATE201238T1 (de)
AU (1) AU685628B2 (de)
CA (1) CA2163662A1 (de)
DE (1) DE69520917T2 (de)
DK (1) DK0703991T3 (de)
ES (1) ES2159635T3 (de)
GR (1) GR3036314T3 (de)
PT (1) PT703991E (de)
WO (1) WO1995027080A2 (de)

Families Citing this family (339)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0630265B1 (de) * 1992-03-13 2003-04-16 Diatide, Inc. Technetium-99-markierte peptide zur visualisierung vom entzuendungen
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5695934A (en) * 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6974666B1 (en) * 1994-10-21 2005-12-13 Appymetric, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US5695937A (en) * 1995-09-12 1997-12-09 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5866330A (en) 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US5763175A (en) * 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5851772A (en) * 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
HUP0003944A3 (en) * 1996-06-06 2003-08-28 Lynx Therapeutics Inc Hayward Sequencing by ligation of encoded adaptors
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
US6124092A (en) * 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US6258533B1 (en) * 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
EP1009802B1 (de) * 1997-02-12 2004-08-11 Eugene Y. Chan Verfahren zur analyse von polymeren
IL120339A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US5994068A (en) 1997-03-11 1999-11-30 Wisconsin Alumni Research Foundation Nucleic acid indexing
EP1591541B1 (de) 1997-04-01 2012-02-15 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
US5888737A (en) * 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
EP0985142A4 (de) 1997-05-23 2006-09-13 Lynx Therapeutics Inc System und gerät zur sequentiellen behandlung von analyten
ATE289358T1 (de) * 1997-06-27 2005-03-15 Lynx Therapeutics Inc Verfahren zur restriktionskarten-analyse von polynukleotiden
JP4789294B2 (ja) * 1997-08-22 2011-10-12 ソレクサ・インコーポレイテッド ローリングプライマーを用いたｄｎａ伸長および分析
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
GB9803930D0 (en) * 1998-02-26 1998-04-22 Univ Glasgow Polynucleotide sequencing method and kits therefor
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
DE19822287C2 (de) * 1998-05-18 2003-04-24 Switch Biotech Ag Klonierungsvektor, seine Herstellung und Verwendung zur Analyse von mRNA Expressionsmuster
US7060431B2 (en) 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
US6162602A (en) * 1998-07-16 2000-12-19 Gautsch; James W. Automatic direct sequencing of bases in nucleic acid chain elongation
US7270958B2 (en) * 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
US6500650B1 (en) 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
US6566059B1 (en) 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6855500B2 (en) 1998-10-01 2005-02-15 Sequenom, Inc. Fluorescence-based genotyping
JP4638043B2 (ja) 1998-12-14 2011-02-23 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
AU2467800A (en) * 1999-01-29 2000-08-18 Keygene N.V. Method and arrangement for determining one or more restriction enzymes for analyzing a nucleic acid or nucleic acid sequence
AU3171700A (en) * 1999-03-05 2000-09-28 Transgene Nucleotide fragment, probe, primer, reagent and method for detecting a nucleotide sequence derived from pbr322 replication origin
US6521428B1 (en) * 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
EP2360270B1 (de) 1999-05-20 2016-11-09 Illumina, Inc. Kombinatorische Entschlüsselung von Zufallsnukleinsäurearrays
US20020119448A1 (en) * 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
JP2003527087A (ja) * 1999-08-13 2003-09-16 イェール・ユニバーシティ バイナリーコード化配列タグ
GB0016473D0 (en) * 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
GB0016472D0 (en) * 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
US20060057565A1 (en) * 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
US7211414B2 (en) * 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US6949340B2 (en) 2001-03-28 2005-09-27 Creative Mines Llc Optical phase modulator
EP1262563A3 (de) * 2001-05-31 2003-02-05 Takara Bio Inc. Methode zur Fixierung von DNA auf Oberflächen
US6613523B2 (en) * 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US20030219770A1 (en) * 2001-11-08 2003-11-27 Eshleman James R. Methods and systems of nucleic acid sequencing
US20030170700A1 (en) * 2002-01-09 2003-09-11 Lynx Therapeutics, Inc. Secreted and cell surface polypeptides affected by cholesterol and uses thereof
US20030166026A1 (en) * 2002-01-09 2003-09-04 Lynx Therapeutics, Inc. Identification of specific biomarkers for breast cancer cells
US20030180764A1 (en) * 2002-01-09 2003-09-25 Lynx Therapeutics, Inc. Genes affected by cholesterol treatment and during adipogenesis
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
WO2003102216A2 (en) * 2002-05-31 2003-12-11 Diversa Corporation Multiplexed systems for nucleic acid sequencing
US7074597B2 (en) * 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
WO2004029220A2 (en) * 2002-09-26 2004-04-08 Kosan Biosciences, Inc. Synthetic genes
WO2004055160A2 (en) * 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
US20040224330A1 (en) * 2003-01-15 2004-11-11 Liyan He Nucleic acid indexing
WO2005010184A1 (ja) * 2003-07-25 2005-02-03 Takara Bio Inc. 変異の検出方法
US7919238B2 (en) * 2003-08-08 2011-04-05 Albert J. Wong Method for rapid identification of alternative splicing
US20050094807A1 (en) * 2003-11-04 2005-05-05 John Silzel Accuracy array assay system and method
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
AU2005214329A1 (en) * 2004-02-12 2005-09-01 Population Genetics Technologies Ltd Genetic analysis by sequence-specific sorting
CA2557818A1 (en) * 2004-03-03 2005-09-15 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
US20050227316A1 (en) * 2004-04-07 2005-10-13 Kosan Biosciences, Inc. Synthetic genes
WO2006073436A2 (en) * 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
GB0422551D0 (en) * 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
EP2236628A3 (de) * 2005-02-01 2010-10-13 AB Advanced Genetic Analysis Corporation Reagentien, Verfahren und Bibliotheken zur Sequenzierung mit Kügelchen
EP2272983A1 (de) 2005-02-01 2011-01-12 AB Advanced Genetic Analysis Corporation Reagentien, Verfahren und Bibliotheken zur Sequenzierung mit Kügelchen
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
WO2006099604A2 (en) * 2005-03-16 2006-09-21 Compass Genetics, Llc Methods and compositions for assay readouts on multiple analytical platforms
WO2007002204A2 (en) 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070065834A1 (en) * 2005-09-19 2007-03-22 Hillis William D Method and sequences for determinate nucleic acid hybridization
CA2630544A1 (en) * 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
WO2007097443A1 (ja) * 2006-02-20 2007-08-30 National University Corporation Hokkaido University Ｄｎａ塩基配列を決定する方法
JP2009538123A (ja) * 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
US20090002703A1 (en) * 2006-08-16 2009-01-01 Craig Edward Parman Methods and systems for quantifying isobaric labels and peptides
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
DE112007002932B4 (de) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren
US7947446B2 (en) * 2007-05-29 2011-05-24 Ming-Sheng Lee High throughput mutation screening methods and kits using a universalized approach—differential sequence fill-in (DSF)-enabled sequential adapter ligation and amplification
WO2009029728A1 (en) 2007-08-29 2009-03-05 Applied Biosystems Inc. Alternative nucleic acid sequencing methods
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
EP4310194A3 (de) 2007-10-19 2024-10-16 The Trustees of Columbia University in the City of New York Entwurf und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
WO2009117031A2 (en) * 2007-12-18 2009-09-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
WO2009086353A1 (en) * 2007-12-26 2009-07-09 Helicos Biosciences Corporation Improved two-primer sequencing for high-throughput expression analysis
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
EP2294214A2 (de) * 2008-05-07 2011-03-16 Illumina, Inc. Zusammensetzungen und verfahren zur bereitstellung von substanzen an und aus einem array
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010068702A2 (en) * 2008-12-10 2010-06-17 Illumina, Inc. Methods and compositions for hybridizing nucleic acids
GB0904934D0 (en) 2009-03-23 2009-05-06 Geneseqe As Method and apparatus for detecting molecules
US20110070590A1 (en) 2009-09-22 2011-03-24 Jan Rohozinski Primers and Methods for Determining RhD Zygosity
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
AU2011237729B2 (en) 2010-04-05 2014-04-03 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US8483969B2 (en) 2010-09-17 2013-07-09 Illuminia, Inc. Variation analysis for multiple templates on a solid support
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
GB201016484D0 (en) 2010-09-30 2010-11-17 Geneseque As Method
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
GB201215435D0 (en) * 2012-08-30 2012-10-17 Geneseque As Sequencing method
US9593365B2 (en) 2012-10-17 2017-03-14 Spatial Transcriptions Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP3553175B1 (de) 2013-03-13 2021-07-14 Illumina, Inc. Verfahren zur herstellung von nukleinsäuresequenzierungsbibliotheken
US20160023208A1 (en) 2013-03-13 2016-01-28 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
KR102230831B1 (ko) 2013-03-15 2021-03-22 일루미나 케임브리지 리미티드 변형된 뉴클레오사이드 또는 뉴클레오타이드
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
MX363806B (es) 2013-07-01 2019-04-03 Illumina Inc Funcionalización de superficies sin catalizador e injerto de polímeros..
EP3241913B1 (de) 2013-07-03 2019-02-20 Illumina, Inc. System zur sequenzierung durch orthogonale synthese
JP2016539343A (ja) 2013-08-30 2016-12-15 イルミナ インコーポレイテッド 親水性または斑状親水性表面上の液滴の操作
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
US10246746B2 (en) 2013-12-20 2019-04-02 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
KR102333635B1 (ko) 2013-12-23 2021-11-30 일루미나, 인코포레이티드 광 방출의 검출을 개선시키기 위한 구조화 기판 및 이와 관련한 방법
US10934581B2 (en) 2014-04-30 2021-03-02 International Business Machines Corporation Bow tie DNA compositions and methods
WO2015189636A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
ES2697428T3 (es) 2014-07-15 2019-01-23 Illumina Inc Dispositivo electrónico activado bioquímicamente
GB201413929D0 (en) 2014-08-06 2014-09-17 Geneseque As Method
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
EP3183577B1 (de) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible oberflächenfunktionalisierung
JP6808617B2 (ja) 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
CN114805710B (zh) 2014-10-31 2023-11-17 伊鲁米纳剑桥有限公司 聚合物以及dna共聚物涂层
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
CN108449971B (zh) 2015-04-14 2020-10-16 亿明达股份有限公司 用于改进对光发射的检测的结构化基底及涉及其的方法
JP2018518157A (ja) 2015-05-11 2018-07-12 イラミーナ インコーポレーテッド 治療薬剤の発見および分析のためのプラットフォーム
CN107924121B (zh) 2015-07-07 2021-06-08 亿明达股份有限公司 经由纳米压印的选择性表面图案化
WO2017015018A1 (en) 2015-07-17 2017-01-26 Illumina, Inc. Polymer sheets for sequencing applications
AU2016298541B2 (en) 2015-07-30 2019-10-31 Illumina, Inc. Orthogonal deblocking of nucleotides
CN107921432A (zh) 2015-09-02 2018-04-17 伊卢米纳剑桥有限公司 改善流控系统中的液滴操作的系统和方法
CN109072300B (zh) 2015-12-17 2023-01-31 伊路敏纳公司 区分复杂生物样品中的甲基化水平
US10907195B1 (en) * 2016-02-16 2021-02-02 Pharmaseq, Inc. Devices and methods for assembling extremely long DNA molecules
PL3377226T3 (pl) 2016-03-28 2021-07-26 Illumina, Inc. Wielopłaszczyznowe mikromacierze
WO2017177017A1 (en) 2016-04-07 2017-10-12 Omniome, Inc. Methods of quantifying target nucleic acids and identifying sequence variants
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CA3050241C (en) 2017-01-20 2022-11-29 Omniome, Inc. Allele-specific capture of nucleic acids
EP3565900B1 (de) 2017-01-20 2021-04-07 Omniome, Inc. Genotypisierung durch polymerasebindung
US10737267B2 (en) 2017-04-04 2020-08-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
SG11202000964UA (en) 2017-08-15 2020-02-27 Omniome Inc Scanning apparatus and methods useful for detection of chemical and biological analytes
KR20200059243A (ko) 2017-09-20 2020-05-28 리제너론 파마슈티칼스 인코포레이티드 패신저 유전자 돌연변이 부담이 높은 종양을 가진 환자에 대한 면역 치료요법
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
NL2021377B1 (en) 2018-07-03 2020-01-08 Illumina Inc Interposer with first and second adhesive layers
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3844308A1 (de) 2018-08-28 2021-07-07 10X Genomics, Inc. Auflösung von räumlichen anordnungen
EP4603594A3 (de) 2018-08-28 2025-11-12 10X Genomics, Inc. Erhöhung der räumlichen array-auflösung
AU2019364418B2 (en) 2018-10-25 2025-11-13 Illumina, Inc. Methods and compositions for identifying ligands on arrays using indexes and barcodes
CN211311438U (zh) 2018-12-04 2020-08-21 欧姆尼欧美公司 流动池
AU2019391274B2 (en) 2018-12-05 2025-10-30 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
US20220290217A1 (en) 2018-12-10 2022-09-15 10X Genomics, Inc. Resolving spatial arrays using deconvolution
JP7587425B2 (ja) 2018-12-18 2024-11-20 イルミナ ケンブリッジ リミテッド 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物
BR112020026667A2 (pt) 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020167574A1 (en) 2019-02-14 2020-08-20 Omniome, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
CN114127309A (zh) 2019-03-15 2022-03-01 10X基因组学有限公司 使用空间阵列进行单细胞测序的方法
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
WO2020252186A1 (en) 2019-06-11 2020-12-17 Omniome, Inc. Calibrated focus sensing
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
TW202124406A (zh) 2019-09-10 2021-07-01 美商歐姆尼歐美公司 核苷酸之可逆修飾
EP4031682A1 (de) 2019-09-20 2022-07-27 Illumina, Inc. Verfahren und zusammensetzungen zur identifizierung von liganden auf arrays mit indizes und barcodes
CN117036248A (zh) 2019-10-01 2023-11-10 10X基因组学有限公司 用于识别组织样品中的形态学模式的系统和方法
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
EP4055185A1 (de) 2019-11-08 2022-09-14 10X Genomics, Inc. Räumlich markierte analytfänger für analyt-multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CA3158603A1 (en) 2019-11-18 2021-05-27 Neil Ira WEISENFELD Systems and methods for tissue classification
US20210155982A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc. Pipeline for spatial analysis of analytes
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
EP4081656A1 (de) 2019-12-23 2022-11-02 10X Genomics, Inc. Zusammensetzungen und verfahren zur verwendung von fixierten biologischen proben bei partitionsbasierten tests
AU2020412459B2 (en) 2019-12-23 2022-12-08 Singular Genomics Systems, Inc. Methods for long read sequencing
EP4081777B1 (de) 2019-12-23 2026-02-18 10X Genomics, Inc. Reversible fixierreagenzien und verfahren zu deren verwendung
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
WO2021158511A1 (en) 2020-02-04 2021-08-12 Omniome, Inc. Flow cells and methods for their manufacture and use
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12421558B2 (en) 2020-02-13 2025-09-23 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and DNA chromatin accessibility
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
EP4484571A3 (de) 2020-02-21 2025-03-19 10X Genomics, Inc. Verfahren und zusammensetzungen für integrierten räumlichen in-situ-assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CA3173798A1 (en) 2020-03-03 2021-09-10 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
US11359238B2 (en) 2020-03-06 2022-06-14 Singular Genomics Systems, Inc. Linked paired strand sequencing
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
CN116134308B (zh) 2020-05-19 2025-05-27 10X基因组学有限公司 电泳盒和仪器
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459B1 (de) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4158054B1 (de) 2020-06-02 2025-04-16 10X Genomics, Inc. Räumliche transkriptomik für antigenrezeptoren
WO2021247543A2 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Nucleic acid library methods
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US20230193356A1 (en) 2020-06-08 2023-06-22 The Broad Institute, Inc. Single cell combinatorial indexing from amplified nucleic acids
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
EP4446430A3 (de) 2020-06-10 2024-12-18 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
EP4153606A4 (de) 2020-07-13 2024-10-02 Singular Genomics Systems, Inc. Verfahren zur sequenzierung komplementärer polynukleotide
EP4121561A4 (de) 2020-08-06 2024-04-17 Singular Genomics Systems, Inc. Verfahren für in-situ-transkriptomik und proteomik
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US12580044B1 (en) 2020-09-02 2026-03-17 10X Genomics, Inc. Systems and methods for identifying cells that are antigen-specific for an immunogenic feature
WO2022061150A2 (en) 2020-09-18 2022-03-24 10X Geonomics, Inc. Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022087485A1 (en) 2020-10-22 2022-04-28 Singular Genomics Systems, Inc. Nucleic acid circularization and amplification on a surface
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US12509717B2 (en) 2020-12-11 2025-12-30 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4121555B1 (de) 2020-12-21 2026-02-25 10X Genomics, Inc. Verfahren zur erfassung von sonden und/oder barcodes
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284945B1 (de) 2021-01-26 2024-10-23 10X Genomics, Inc. Nukleinsäureanalogsonden zur in-situ-analyse
US11486001B2 (en) 2021-02-08 2022-11-01 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
WO2022174054A1 (en) 2021-02-13 2022-08-18 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
EP4421491A3 (de) 2021-02-19 2024-11-27 10X Genomics, Inc. Verfahren zur verwendung einer modularen testträgervorrichtung
EP4301873A1 (de) 2021-03-03 2024-01-10 10X Genomics, Inc. In-situ-analytnachweis mit nukleinsäureorigami
EP4263868A4 (de) 2021-03-12 2024-11-27 Singular Genomics Systems, Inc. Nanoarrays und verfahren zur verwendung davon
US11884977B2 (en) 2021-03-12 2024-01-30 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
CA3210451A1 (en) 2021-03-22 2022-09-29 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
EP4428246B1 (de) 2021-04-14 2025-12-24 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
US11578320B2 (en) 2021-04-27 2023-02-14 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
WO2022235764A1 (en) 2021-05-05 2022-11-10 Singular Genomics Systems, Inc. Multiomic analysis device and methods of use thereof
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
EP4347877A1 (de) 2021-06-01 2024-04-10 10X Genomics, Inc. Verfahren und zusammensetzungen zum nachweis von analyten und zur sondenauflösung
EP4347880B1 (de) 2021-06-02 2026-02-04 10X Genomics, Inc. Probenanalyse mit asymmetrischen zirkularisierbaren sonden
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
WO2023023484A1 (en) 2021-08-16 2023-02-23 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
WO2023023638A1 (en) 2021-08-20 2023-02-23 Singular Genomics Systems, Inc. Chemical and thermal assisted nucleic acid amplification methods
CN118103750A (zh) 2021-08-31 2024-05-28 伊鲁米纳公司 具有增强的孔成像分辨率的流动池
EP4196605B1 (de) 2021-09-01 2024-12-04 10X Genomics, Inc. Verfahren zur blockierung einer erfassungssonde auf einer räumlichen anordnung
EP4423664A4 (de) 2021-10-25 2025-08-27 Singular Genomics Systems Inc Manipulation und detektion biologischer proben
EP4422792A4 (de) 2021-10-26 2025-12-17 Singular Genomics Systems Inc Gezielte multiplex-amplifikation von polynukleotiden
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (de) 2021-12-20 2024-10-09 10X Genomics, Inc. Selbsttest für eine pathologie-/histologie-objektträgerbildgebungsvorrichtung
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230212667A1 (en) 2021-12-29 2023-07-06 Illumina Cambridge Limited Methods of nucleic acid sequencing using surface-bound primers
WO2023141588A1 (en) 2022-01-21 2023-07-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
US11795505B2 (en) 2022-03-10 2023-10-24 Singular Genomics Systems, Inc. Nucleic acid delivery scaffolds
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
EP4504969A1 (de) 2022-04-06 2025-02-12 10X Genomics, Inc. Verfahren zur multiplex-zellanalyse
EP4519674A1 (de) 2022-05-06 2025-03-12 10X Genomics, Inc. Analyse von antigen- und antigenrezeptorinteraktionen
EP4540607A1 (de) 2022-06-17 2025-04-23 10X Genomics, Inc. Katalytische entvernetzung von proben zur in-situ-analyse
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
WO2024036304A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Puma1 polymerases and uses thereof
US12116626B2 (en) 2022-08-16 2024-10-15 10X Genomics, Inc. AP50 polymerases and uses thereof
CN120129825A (zh) 2022-10-14 2025-06-10 10X基因组学有限公司 用于评估生物样品质量的方法、组合物和系统
WO2024102736A1 (en) 2022-11-08 2024-05-16 10X Genomics, Inc. Immobilization methods and compositions for in situ detection
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
EP4573210B1 (de) 2022-11-16 2026-05-06 10X Genomics, Inc. Verfahren zur analyse der genexprimierung in zellen in einem pellet
WO2024107727A1 (en) 2022-11-18 2024-05-23 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
EP4622746A1 (de) 2022-11-22 2025-10-01 10X Genomics, Inc. Systeme und verfahren zur schnittstellenbildung mit einer flasche
US12581050B2 (en) 2022-11-23 2026-03-17 10X Genomics, Inc. Optical assemblies for machine vision calibration
WO2024129672A1 (en) 2022-12-12 2024-06-20 The Broad Institute, Inc. Trafficked rnas for assessment of cell-cell connectivity and neuroanatomy
WO2024129969A1 (en) 2022-12-14 2024-06-20 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
EP4638789A1 (de) 2022-12-21 2025-10-29 10X Genomics, Inc. Verfahren zur bindung von ribonukleinsäuren in biologischen proben
WO2024229260A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
US20240376521A1 (en) 2023-05-10 2024-11-14 10X Genomics, Inc. Boronic acid compositions and methods for tethering ribonucleic acids in biological samples
EP4720339A1 (de) 2023-05-31 2026-04-08 Illumina, Inc. Verfahren zur doppelsträngigen sequenzierung durch synthese
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
US20250163502A1 (en) 2023-11-22 2025-05-22 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025194033A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
WO2025207535A1 (en) 2024-03-26 2025-10-02 Illumina, Inc. Photo-switchable surfaces
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
WO2025264702A1 (en) 2024-06-18 2025-12-26 10X Genomics, Inc. 3d hydrogels for nucleic acid sequencing
WO2025264836A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2025264831A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2026006314A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Tagging target regions prior to nucleotide sequencing
WO2026043885A1 (en) 2024-08-20 2026-02-26 10X Genomics, Inc. Methods and systems for preparing a nucleic acid library
WO2026090042A1 (en) 2024-10-21 2026-04-30 The Broad Institute, Inc. Single-cell and spatial dna methylation measurements
WO2026087220A1 (en) 2024-10-22 2026-04-30 F. Hoffmann-La Roche Ag Preparation of dna libraries for next generation sequencing using y-adapters modified with phosphorothioate bonds
WO2026093331A1 (en) 2024-10-28 2026-05-07 F. Hoffmann-La Roche Ag Hairpin adapter constructs and primers to generate next-generation sequencing library molecules

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
US4321365A (en) * 1977-10-19 1982-03-23 Research Corporation Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
US4293652A (en) * 1979-05-25 1981-10-06 Cetus Corporation Method for synthesizing DNA sequentially
GB2258950B (en) * 1982-05-11 1993-07-07 Secr Defence Integrated antenna device with resistive connection
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5242794A (en) * 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0224126A3 (de) * 1985-11-25 1989-02-01 The University of Calgary Kovalent gekoppelte komplementäre Oligodeoxynukleotide zur Verwendung als "Primer Linkers" bei Sequenzierung von Nukleinsäuren
GB8612087D0 (en) * 1986-05-19 1986-06-25 Ici Plc Hybridisation probes
US5202231A (en) * 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US5102785A (en) * 1987-09-28 1992-04-07 E. I. Du Pont De Nemours And Company Method of gene mapping
US5093245A (en) * 1988-01-26 1992-03-03 Applied Biosystems Labeling by simultaneous ligation and restriction
US5002867A (en) * 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
WO1989011211A2 (en) * 1988-05-24 1989-11-30 Gesellschaft Für Biotechnologische Forschung Mbh ( Oligonucleotide bank and process for dna sequencing
US5489507A (en) * 1988-11-30 1996-02-06 Perkin-Elmer Corporation DNA detection by color complementation
JPH02299598A (ja) * 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5427911A (en) * 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
DE4321198A1 (de) * 1993-06-25 1995-01-05 Henkel Kgaa Verwendung von N-Allylaminoaromaten in Oxidationsfärbemitteln
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids

Also Published As

Publication number Publication date
DE69520917T2 (de) 2001-12-20
US5552278A (en) 1996-09-03
JP2005323607A (ja) 2005-11-24
AU2379195A (en) 1995-10-23
GR3036314T3 (en) 2001-10-31
WO1995027080A2 (en) 1995-10-12
US5856093A (en) 1999-01-05
JP3872812B2 (ja) 2007-01-24
US5599675A (en) 1997-02-04
AU685628B2 (en) 1998-01-22
EP0703991A1 (de) 1996-04-03
JPH08511174A (ja) 1996-11-26
JP2006197944A (ja) 2006-08-03
CA2163662A1 (en) 1995-10-12
EP0703991B1 (de) 2001-05-16
ES2159635T3 (es) 2001-10-16
PT703991E (pt) 2001-10-30
DE69520917D1 (de) 2001-06-21
DK0703991T3 (da) 2001-06-18
JP4250174B2 (ja) 2009-04-08
WO1995027080A3 (en) 1995-11-16

Similar Documents

Publication Publication Date Title
DE69520917D1 (de) Dns-sequenzierung durch stufenweise ligierung und spaltung
CA2218017A1 (en) Dna sequencing by parallel oligonucleotide extensions
DE69432419D1 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
DE69737450D1 (de) Sequenzierung durch ligation kodierter adapter
DE60005309D1 (de) Methode zur sequenzierung von polynukleotiden
ATE250671T1 (de) Verfahren mit einer hohen umsatzrate für das auffinden von sequenzen oder genetischen veränderungen in nukleinsäuren
DK1159453T3 (da) Fremgangsmåde til direkte nukleinsyresekventering
JO1598B1 (en) A method for amplifying and amplifying the biocleotide sequences
DE69917303D1 (de) Genomische multi-loci analyse mittels eines verbesserten verfahrens basierend auf zyklischer dna-sequenzierung
DE3587427D1 (de) Hybridisierungsverfahren zum nachweis genetischer stoffe.
DE60113945D1 (de) Verfahren zur Analyse von Wiederkehrenden PCR-Podukten, das auf der Analyse von DNS-Schmelzkurven basiert
WO2003052099A3 (en) Methods of parallel gene cloning and analysis
DK0898618T3 (da) Fremgangsmåde til tilvejebringelse af hidtil ukendte DNA-sekvenser
DE69940890D1 (de) Ein verfahren zur analyse von polynukleotiden
SE8700821D0 (sv) Quantitative determination of nucleic acid molecules and the reagent kit used
DE69323199D1 (de) Gen für die ataxia-telangiectasia komplementationsgruppe d
TW329476B (en) Method for detecting contaminant DNA in a protein product and kits used for the same purpose
ATE211180T1 (de) Verfahren zur nicht-radioaktiven detektion von membran-gebundenen nukleinsäuren und testkit
Brenner DNA sequencing by stepwise ligation and cleavage
DE69009050D1 (de) Verfahren zur stabilisierung der hybridisierung von komplementären polynukleotidsequenzen.
ATE108834T1 (de) Spezifische nukleinsäurefragmente des menschlichen villingens, ihre anwendung für diagnostische zwecke.
Luk'ianov et al. In vitro cloning of DNA fragments by one polymerase chain reaction
Subramanian et al. SEQUENCES OF SV40 DNA
KR960023084A (ko) 수두 바이러스 게놈 디엔에이(dna)의 비교정량적 피씨알(pcr) 방법
WO1996033284A3 (en) Nucleic acid sequencing

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee