AT502823B1 - Polynukleotid-amplifikation - Google Patents

Polynukleotid-amplifikation Download PDF

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Publication number
AT502823B1
AT502823B1 AT0192305A AT19232005A AT502823B1 AT 502823 B1 AT502823 B1 AT 502823B1 AT 0192305 A AT0192305 A AT 0192305A AT 19232005 A AT19232005 A AT 19232005A AT 502823 B1 AT502823 B1 AT 502823B1
Authority
AT
Austria
Prior art keywords
primer
rna
cdna
amplification
tail
Prior art date
Application number
AT0192305A
Other languages
German (de)
English (en)
Other versions
AT502823A4 (de
Original Assignee
Seitz Alexander Dr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AT0192305A priority Critical patent/AT502823B1/de
Application filed by Seitz Alexander Dr filed Critical Seitz Alexander Dr
Priority to EP06827949A priority patent/EP1954829B1/en
Priority to PCT/AT2006/000494 priority patent/WO2007062445A1/en
Priority to CA2630573A priority patent/CA2630573C/en
Priority to DE602006014860T priority patent/DE602006014860D1/de
Priority to CN200680044723.5A priority patent/CN101316937B/zh
Priority to US12/095,485 priority patent/US9394564B2/en
Priority to AT06827949T priority patent/ATE470726T1/de
Priority to JP2008542554A priority patent/JP5118056B2/ja
Priority to KR1020087015868A priority patent/KR101327376B1/ko
Application granted granted Critical
Publication of AT502823B1 publication Critical patent/AT502823B1/de
Publication of AT502823A4 publication Critical patent/AT502823A4/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/131—Terminal transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
    • C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
    • C12Q2539/113—Differential Display Analysis [DDA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT0192305A 2005-11-29 2005-11-29 Polynukleotid-amplifikation AT502823B1 (de)

Priority Applications (10)

Application Number Priority Date Filing Date Title
AT0192305A AT502823B1 (de) 2005-11-29 2005-11-29 Polynukleotid-amplifikation
PCT/AT2006/000494 WO2007062445A1 (en) 2005-11-29 2006-11-29 Polynucleotide amplification
CA2630573A CA2630573C (en) 2005-11-29 2006-11-29 Polynucleotide amplification
DE602006014860T DE602006014860D1 (de) 2005-11-29 2006-11-29 Polynukleotidamplifikation
EP06827949A EP1954829B1 (en) 2005-11-29 2006-11-29 Polynucleotide amplification
CN200680044723.5A CN101316937B (zh) 2005-11-29 2006-11-29 多核苷酸扩增
US12/095,485 US9394564B2 (en) 2005-11-29 2006-11-29 Polynucleotide amplification
AT06827949T ATE470726T1 (de) 2005-11-29 2006-11-29 Polynukleotidamplifikation
JP2008542554A JP5118056B2 (ja) 2005-11-29 2006-11-29 ポリヌクレオチド増幅
KR1020087015868A KR101327376B1 (ko) 2005-11-29 2006-11-29 폴리뉴클레오티드 증폭

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT0192305A AT502823B1 (de) 2005-11-29 2005-11-29 Polynukleotid-amplifikation

Publications (2)

Publication Number Publication Date
AT502823B1 true AT502823B1 (de) 2007-06-15
AT502823A4 AT502823A4 (de) 2007-06-15

Family

ID=37814243

Family Applications (2)

Application Number Title Priority Date Filing Date
AT0192305A AT502823B1 (de) 2005-11-29 2005-11-29 Polynukleotid-amplifikation
AT06827949T ATE470726T1 (de) 2005-11-29 2006-11-29 Polynukleotidamplifikation

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT06827949T ATE470726T1 (de) 2005-11-29 2006-11-29 Polynukleotidamplifikation

Country Status (9)

Country Link
US (1) US9394564B2 (enExample)
EP (1) EP1954829B1 (enExample)
JP (1) JP5118056B2 (enExample)
KR (1) KR101327376B1 (enExample)
CN (1) CN101316937B (enExample)
AT (2) AT502823B1 (enExample)
CA (1) CA2630573C (enExample)
DE (1) DE602006014860D1 (enExample)
WO (1) WO2007062445A1 (enExample)

Families Citing this family (20)

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CN102597265A (zh) * 2009-10-21 2012-07-18 里博克斯艾克斯有限公司 使用热稳定的rna依赖的rna聚合酶进行指数式扩增rna的方法
EP2333104A1 (en) 2009-12-11 2011-06-15 Lexogen GmbH RNA analytics method
EP2354243A1 (en) 2010-02-03 2011-08-10 Lexogen GmbH Complexity reduction method
PL2609118T3 (pl) 2010-08-23 2017-07-31 Board Of Regents, The University Of Texas System Przeciwciała anty-OX40 i sposoby ich stosowania
DK2630263T4 (da) 2010-10-22 2022-02-14 Cold Spring Harbor Laboratory Varital tælling af nucleinsyrer for at opnå information om antal genomiske kopier
EP2492673A1 (en) 2011-02-28 2012-08-29 Lexogen GmbH Biosensor array formed by junctions of functionalized electrodes
EP2492674A1 (en) 2011-02-28 2012-08-29 Lexogen GmbH Biosensor array formed by junctions of functionalized electrodes
CN103160575B (zh) * 2011-12-15 2014-10-01 王荣芳 SAA1β/β纯合子基因型在肝硬化预后诊断及肝硬化诊断中的应用
DK2828399T3 (en) * 2012-03-22 2017-04-10 Lgc Genomics Ltd SYSTEM FOR DETECTING POLYMERASE CHAIN REACTION USING OLIGONUCLEOTIDS INCLUDING A PHOSPHOROTHIOATE GROUP
EP2684954A1 (en) 2012-07-10 2014-01-15 Lexogen GmbH 5´ protection dependent amplification
WO2014066179A1 (en) * 2012-10-24 2014-05-01 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
KR101922125B1 (ko) 2012-11-29 2018-11-26 삼성전자주식회사 표적 핵산을 표지하는 방법
EP2921556A1 (en) 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
CN106978506A (zh) * 2017-05-05 2017-07-25 武汉爱基百客生物科技有限公司 一种检测大肠杆菌的荧光定量pcr方法
EP3673064A4 (en) * 2017-08-24 2021-05-26 Takara Bio USA, Inc. METHOD OF PRODUCING NUCLEIC ACIDS USING STIMULUS MODIFIED OLIGONUCLEOTIDES
CN108949937A (zh) * 2018-07-16 2018-12-07 翌圣生物科技（上海）有限公司 一步法rt-pcr预混试剂
AU2019321683B2 (en) * 2018-08-17 2025-04-03 Dana-Farber Cancer Institute, Inc. DNA amplification method for probe generation
WO2020132103A1 (en) * 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority
EP3940073A4 (en) * 2019-03-13 2023-03-01 Toyobo Co., Ltd. PRODUCTION AND AMPLIFICATION OF NUCLEIC ACIDS
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018176A1 (en) * 1992-03-11 1993-09-16 Dana-Farber Cancer Institute METHODS TO CLONE mRNA
WO1996040998A1 (en) * 1995-06-07 1996-12-19 Pioneer Hi-Bred International, Inc. PCR-BASED cDNA SUBTRACTIVE CLONING METHOD
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
US20010012922A1 (en) * 1998-11-13 2001-08-09 Cimino William W. Protective sheath and method for ultrasonic probes
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US20020177701A1 (en) * 1995-08-01 2002-11-28 Yale University Analysis of gene expression by display of 3'-end fragments of cDNAs
US20030108874A1 (en) * 2000-11-01 2003-06-12 Genomic Solutions, Inc. Compositions and systems for identifying and comparing expressed genes (mRNAs) in eukaryotic organisms
US20040166499A1 (en) * 2000-10-05 2004-08-26 Yoshihide Hayashizaki Oligonucleotide linkers comprising a variable cohesive portion and method for the praparation of polynucleotide libraries by using said linkers

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AU632760B2 (en) * 1988-07-26 1993-01-14 Genelabs Technologies, Inc. Rna and dna amplification techniques
US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
AU2264197A (en) * 1996-02-09 1997-08-28 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Restriction display (rd-pcr) of differentially expressed mrnas
US20020012922A1 (en) 1998-11-04 2002-01-31 Hilbush Brian S. Simplified method for indexing and determining the relative concentration of expressed messenger RNAs
DE19920611A1 (de) * 1999-05-05 2000-11-09 Roche Diagnostics Gmbh Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs
EP1235932A2 (en) * 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
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US20030077611A1 (en) * 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
EP1369480A1 (en) 2002-06-05 2003-12-10 Bioneer Corporation Process for preparation of full-length cDNA and anchor used for the same
WO2004087952A2 (en) * 2003-03-31 2004-10-14 Vib Vzw Genes regulated by hydrogen peroxide stress

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018176A1 (en) * 1992-03-11 1993-09-16 Dana-Farber Cancer Institute METHODS TO CLONE mRNA
WO1996040998A1 (en) * 1995-06-07 1996-12-19 Pioneer Hi-Bred International, Inc. PCR-BASED cDNA SUBTRACTIVE CLONING METHOD
US20020177701A1 (en) * 1995-08-01 2002-11-28 Yale University Analysis of gene expression by display of 3'-end fragments of cDNAs
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US20010012922A1 (en) * 1998-11-13 2001-08-09 Cimino William W. Protective sheath and method for ultrasonic probes
US20040166499A1 (en) * 2000-10-05 2004-08-26 Yoshihide Hayashizaki Oligonucleotide linkers comprising a variable cohesive portion and method for the praparation of polynucleotide libraries by using said linkers
US20030108874A1 (en) * 2000-11-01 2003-06-12 Genomic Solutions, Inc. Compositions and systems for identifying and comparing expressed genes (mRNAs) in eukaryotic organisms

Also Published As

Publication number Publication date
CN101316937B (zh) 2016-05-25
CA2630573C (en) 2016-03-15
JP5118056B2 (ja) 2013-01-16
US20090311754A1 (en) 2009-12-17
KR101327376B1 (ko) 2013-11-08
DE602006014860D1 (de) 2010-07-22
US9394564B2 (en) 2016-07-19
CN101316937A (zh) 2008-12-03
WO2007062445A1 (en) 2007-06-07
JP2009517052A (ja) 2009-04-30
ATE470726T1 (de) 2010-06-15
EP1954829A1 (en) 2008-08-13
EP1954829B1 (en) 2010-06-09
AT502823A4 (de) 2007-06-15
KR20080080600A (ko) 2008-09-04
CA2630573A1 (en) 2007-06-07

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