DE602006014860D1 - Polynukleotidamplifikation - Google Patents
PolynukleotidamplifikationInfo
- Publication number
- DE602006014860D1 DE602006014860D1 DE602006014860T DE602006014860T DE602006014860D1 DE 602006014860 D1 DE602006014860 D1 DE 602006014860D1 DE 602006014860 T DE602006014860 T DE 602006014860T DE 602006014860 T DE602006014860 T DE 602006014860T DE 602006014860 D1 DE602006014860 D1 DE 602006014860D1
- Authority
- DE
- Germany
- Prior art keywords
- cdna
- sample
- polynucleotide
- primer
- obtaining
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/131—Terminal transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
- C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
- C12Q2539/113—Differential Display Analysis [DDA]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT0192305A AT502823B1 (de) | 2005-11-29 | 2005-11-29 | Polynukleotid-amplifikation |
| PCT/AT2006/000494 WO2007062445A1 (en) | 2005-11-29 | 2006-11-29 | Polynucleotide amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602006014860D1 true DE602006014860D1 (de) | 2010-07-22 |
Family
ID=37814243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602006014860T Active DE602006014860D1 (de) | 2005-11-29 | 2006-11-29 | Polynukleotidamplifikation |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US9394564B2 (de) |
| EP (1) | EP1954829B1 (de) |
| JP (1) | JP5118056B2 (de) |
| KR (1) | KR101327376B1 (de) |
| CN (1) | CN101316937B (de) |
| AT (2) | AT502823B1 (de) |
| CA (1) | CA2630573C (de) |
| DE (1) | DE602006014860D1 (de) |
| WO (1) | WO2007062445A1 (de) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2491131A1 (de) * | 2009-10-21 | 2012-08-29 | Riboxx GmbH | Verfahren zur exponentiellen rna-amplifikation mit wärmestabiler rna-abhängiger rna-polymerase |
| EP2333104A1 (de) | 2009-12-11 | 2011-06-15 | Lexogen GmbH | RNA-Analyseverfahren |
| EP2354243A1 (de) | 2010-02-03 | 2011-08-10 | Lexogen GmbH | Verfahren zur Komplexitätsminderung |
| BR112013004266A8 (pt) | 2010-08-23 | 2018-01-02 | Univ Texas | anticorpos anti-ox40 e métodos de uso dos mesmos. |
| SI2630263T2 (sl) * | 2010-10-22 | 2022-04-29 | Cold Spring Harbor Laboratory | Vrstno štetje nukleinskih kislin za pridobivanje informacij o številu genomskih kopij |
| EP2492674A1 (de) | 2011-02-28 | 2012-08-29 | Lexogen GmbH | Biosensorarray gebildet aus Knotenpunkten zwischen funktionalisierten Elektroden |
| EP2492673A1 (de) | 2011-02-28 | 2012-08-29 | Lexogen GmbH | Biosensorarray gebildet aus Knotenpunkten zwischen funktionalisierten Elektroden |
| CN103160575B (zh) * | 2011-12-15 | 2014-10-01 | 王荣芳 | SAA1β/β纯合子基因型在肝硬化预后诊断及肝硬化诊断中的应用 |
| CN104379763B (zh) * | 2012-03-22 | 2017-04-19 | Lgc基因组学有限公司 | 使用包含硫代磷酸酯基的寡核苷酸的聚合酶链式反应检测系统 |
| EP2684954A1 (de) | 2012-07-10 | 2014-01-15 | Lexogen GmbH | 5'-schutzabhängige Verstärkung |
| EP2912197B1 (de) * | 2012-10-24 | 2019-08-07 | Takara Bio USA, Inc. | Auf vorlagenwechseln basierende verfahren zur herstellung einer produktnukleinsäure |
| KR101922125B1 (ko) | 2012-11-29 | 2018-11-26 | 삼성전자주식회사 | 표적 핵산을 표지하는 방법 |
| EP2921556A1 (de) | 2014-03-21 | 2015-09-23 | Lexogen GmbH | Anzahlerhaltendes RNA-Analyseverfahren |
| CN106978506A (zh) * | 2017-05-05 | 2017-07-25 | 武汉爱基百客生物科技有限公司 | 一种检测大肠杆菌的荧光定量pcr方法 |
| US11667952B2 (en) | 2017-08-24 | 2023-06-06 | Takara Bio Usa, Inc. | Methods of producing nucleic acids using oligonucleotides modified by a stimulus |
| CN108949937A (zh) * | 2018-07-16 | 2018-12-07 | 翌圣生物科技（上海）有限公司 | 一步法rt-pcr预混试剂 |
| AU2019321683A1 (en) * | 2018-08-17 | 2021-01-21 | Dana-Farber Cancer Institute, Inc. | DNA amplification method for probe generation |
| CA3103527A1 (en) * | 2018-12-19 | 2020-06-25 | Illumina, Inc. | Methods for improving polynucleotide cluster clonality priority |
| EP3940073A4 (de) * | 2019-03-13 | 2023-03-01 | Toyobo Co., Ltd. | Herstellung und amplifikation von nukleinsäuren |
| US20210381067A1 (en) * | 2020-06-08 | 2021-12-09 | Ampliwise Inc. | Nucleic acid amplification and detection with attenuaiting probe |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU632760B2 (en) * | 1988-07-26 | 1993-01-14 | Genelabs Technologies, Inc. | Rna and dna amplification techniques |
| US6582908B2 (en) * | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| CA2102784A1 (en) * | 1992-03-11 | 1993-09-12 | Peng Liang | Identifying, isolating and cloning messenger rnas |
| WO1996040998A1 (en) * | 1995-06-07 | 1996-12-19 | Pioneer Hi-Bred International, Inc. | PCR-BASED cDNA SUBTRACTIVE CLONING METHOD |
| US6395887B1 (en) * | 1995-08-01 | 2002-05-28 | Yale University | Analysis of gene expression by display of 3'-end fragments of CDNAS |
| US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| WO1997029211A1 (en) * | 1996-02-09 | 1997-08-14 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs |
| US5932451A (en) * | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
| US6287825B1 (en) * | 1998-09-18 | 2001-09-11 | Molecular Staging Inc. | Methods for reducing the complexity of DNA sequences |
| US20020012922A1 (en) | 1998-11-04 | 2002-01-31 | Hilbush Brian S. | Simplified method for indexing and determining the relative concentration of expressed messenger RNAs |
| US6224565B1 (en) * | 1998-11-13 | 2001-05-01 | Sound Surgical Technologies, Llc | Protective sheath and method for ultrasonic probes |
| DE19920611A1 (de) * | 1999-05-05 | 2000-11-09 | Roche Diagnostics Gmbh | Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs |
| WO2001027327A2 (en) * | 1999-10-08 | 2001-04-19 | Protogene Laboratories, Inc. | Method and apparatus for performing large numbers of reactions using array assembly |
| EP1186673A3 (de) * | 2000-09-11 | 2003-03-26 | Agilent Technologies, Inc. (a Delaware corporation) | Kalibrierung von Daten von Molekül-Arrays |
| US20040166499A1 (en) * | 2000-10-05 | 2004-08-26 | Yoshihide Hayashizaki | Oligonucleotide linkers comprising a variable cohesive portion and method for the praparation of polynucleotide libraries by using said linkers |
| US7290274B1 (en) * | 2000-10-20 | 2007-10-30 | Scientific-Atlanta, Inc. | Context sensitive television menu |
| WO2002036828A2 (en) * | 2000-11-01 | 2002-05-10 | Genomic Solutions Inc. | COMPOSITIONS AND SYSTEMS FOR IDENTIFYING AND COMPARING EXPRESSED GENES (mRNAs) IN EUKARYOTIC ORGANISMS |
| EP1366196A4 (de) * | 2001-02-14 | 2004-07-07 | Baylor College Medicine | Verfahren und zusammensetzungen zur amplifikation von rna |
| US20030077611A1 (en) * | 2001-10-24 | 2003-04-24 | Sention | Methods and systems for dynamic gene expression profiling |
| EP1369480A1 (de) * | 2002-06-05 | 2003-12-10 | Bioneer Corporation | Verfahren zur Herstellung von vollständiger cDNA und darin verwendete Ankersequenz |
| WO2004087952A2 (en) * | 2003-03-31 | 2004-10-14 | Vib Vzw | Genes regulated by hydrogen peroxide stress |
-
2005
- 2005-11-29 AT AT0192305A patent/AT502823B1/de active
-
2006
- 2006-11-29 CN CN200680044723.5A patent/CN101316937B/zh active Active
- 2006-11-29 AT AT06827949T patent/ATE470726T1/de not_active IP Right Cessation
- 2006-11-29 JP JP2008542554A patent/JP5118056B2/ja active Active
- 2006-11-29 CA CA2630573A patent/CA2630573C/en active Active
- 2006-11-29 WO PCT/AT2006/000494 patent/WO2007062445A1/en active Search and Examination
- 2006-11-29 DE DE602006014860T patent/DE602006014860D1/de active Active
- 2006-11-29 EP EP06827949A patent/EP1954829B1/de active Active
- 2006-11-29 KR KR1020087015868A patent/KR101327376B1/ko active IP Right Grant
- 2006-11-29 US US12/095,485 patent/US9394564B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ATE470726T1 (de) | 2010-06-15 |
| EP1954829A1 (de) | 2008-08-13 |
| JP5118056B2 (ja) | 2013-01-16 |
| CA2630573C (en) | 2016-03-15 |
| US9394564B2 (en) | 2016-07-19 |
| US20090311754A1 (en) | 2009-12-17 |
| AT502823B1 (de) | 2007-06-15 |
| AT502823A4 (de) | 2007-06-15 |
| EP1954829B1 (de) | 2010-06-09 |
| WO2007062445A1 (en) | 2007-06-07 |
| KR101327376B1 (ko) | 2013-11-08 |
| CN101316937A (zh) | 2008-12-03 |
| KR20080080600A (ko) | 2008-09-04 |
| CA2630573A1 (en) | 2007-06-07 |
| CN101316937B (zh) | 2016-05-25 |
| JP2009517052A (ja) | 2009-04-30 |
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