ATE480549T1 - Neues verfahren für die bisulfitbehandlung - Google Patents

Neues verfahren für die bisulfitbehandlung

Info

Publication number
ATE480549T1
ATE480549T1 AT06829518T AT06829518T ATE480549T1 AT E480549 T1 ATE480549 T1 AT E480549T1 AT 06829518 T AT06829518 T AT 06829518T AT 06829518 T AT06829518 T AT 06829518T AT E480549 T1 ATE480549 T1 AT E480549T1
Authority
AT
Austria
Prior art keywords
new process
bisulfite treatment
nucleic acid
bisulfite
solid phase
Prior art date
Application number
AT06829518T
Other languages
English (en)
Inventor
Christine Markert-Hahn
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE480549T1 publication Critical patent/ATE480549T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AT06829518T 2005-12-14 2006-12-12 Neues verfahren für die bisulfitbehandlung ATE480549T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05027329 2005-12-14
PCT/EP2006/011932 WO2007068437A1 (en) 2005-12-14 2006-12-12 New method for bisulfite treatment

Publications (1)

Publication Number Publication Date
ATE480549T1 true ATE480549T1 (de) 2010-09-15

Family

ID=35539298

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06829518T ATE480549T1 (de) 2005-12-14 2006-12-12 Neues verfahren für die bisulfitbehandlung

Country Status (8)

Country Link
US (1) US8137937B2 (de)
EP (1) EP1963352B1 (de)
JP (1) JP5374679B2 (de)
CN (1) CN101331148B (de)
AT (1) ATE480549T1 (de)
CA (1) CA2631829C (de)
DE (1) DE602006016859D1 (de)
WO (1) WO2007068437A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003236461B2 (en) * 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法
WO2009070843A1 (en) * 2007-12-05 2009-06-11 Human Genetic Signatures Pty Ltd Bisulphite treatment of rna
GB0814570D0 (en) * 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
US20230148447A9 (en) 2008-12-11 2023-05-11 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
CA2746632C (en) 2008-12-11 2020-06-30 Pacific Biosciences Of California, Inc. Characterization of modified nucleic acids
US9732375B2 (en) 2011-09-07 2017-08-15 Human Genetic Signatures Pty. Ltd. Molecular detection assay using direct treatment with a bisulphite reagent
US9315853B2 (en) * 2012-01-30 2016-04-19 Exact Sciences Corporation Modification of DNA on magnetic beads
US9175348B2 (en) 2012-04-24 2015-11-03 Pacific Biosciences Of California, Inc. Identification of 5-methyl-C in nucleic acid templates
JP6431522B2 (ja) * 2013-03-15 2018-11-28 アボツト・モレキユラー・インコーポレイテツド 核酸の精製のための１工程法
US20160108459A1 (en) * 2014-10-17 2016-04-21 Biochain Institute Inc. Automated isolation and chemical reaction(s) of nucleic acids
US20170253869A1 (en) * 2014-10-17 2017-09-07 Biochain Institute Inc. Automated isolation and chemical reaction(s) of nucleic acids
US10533210B2 (en) 2015-06-10 2020-01-14 Biocartis Nv Detection of methylated DNA
JP7020922B2 (ja) * 2015-06-15 2022-02-16 セファイド 自動反応カートリッジにおける、ＤＮＡメチル化の統合精製及び測定並びに突然変異及び／又はｍＲＮＡ発現レベルの同時測定
AU2017376118B2 (en) 2016-12-12 2022-09-01 Cepheid Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or mRNA expression levels in an automated reaction cartridge
ES2960530T3 (es) * 2017-10-31 2024-03-05 Hoffmann La Roche Partículas magnéticas mejoradas y usos de las mismas
CN115261467A (zh) * 2017-12-01 2022-11-01 博尔诚（北京）科技有限公司 用于检测食道癌的组合物及其用途
CN108265050A (zh) * 2018-03-13 2018-07-10 普迈德（北京）科技有限公司 一种血浆直接亚硫酸氢盐转化的方法及其应用
AU2019249422A1 (en) 2018-04-02 2020-10-15 Grail, Llc Methylation markers and targeted methylation probe panels
CA3111887A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
CA3186515A1 (en) * 2020-07-29 2022-02-24 Evan M. RAGLAND Purification of sulfonated dna
CN116064733B (zh) * 2023-01-18 2024-07-19 昂凯生命科技(苏州)有限公司 用于甲基化检测的样本前处理试剂盒及前处理方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
CA2257109C (en) * 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
DE10029915B4 (de) 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
DE10050942B4 (de) 2000-10-10 2005-11-17 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
US20030152950A1 (en) 2001-06-27 2003-08-14 Garner Harold R. Identification of chemically modified polymers
AU2003236461B2 (en) * 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
DE602004009038T3 (de) 2003-01-29 2015-06-25 Roche Diagnostics Gmbh Verbessertes Verfahren zur Behandlung durch Bisulfit
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
CA2547743C (en) * 2003-12-02 2011-06-28 F. Hoffmann-La Roche Ag Improved method for bisulfite treatment

Also Published As

Publication number Publication date
DE602006016859D1 (de) 2010-10-21
JP5374679B2 (ja) 2013-12-25
CA2631829C (en) 2012-01-24
EP1963352B1 (de) 2010-09-08
JP2009519023A (ja) 2009-05-14
US8137937B2 (en) 2012-03-20
EP1963352A1 (de) 2008-09-03
US20080281087A1 (en) 2008-11-13
WO2007068437A1 (en) 2007-06-21
CN101331148A (zh) 2008-12-24
CA2631829A1 (en) 2007-06-21
CN101331148B (zh) 2012-06-27

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