ATE470726T1 - Polynukleotidamplifikation - Google Patents
PolynukleotidamplifikationInfo
- Publication number
- ATE470726T1 ATE470726T1 AT06827949T AT06827949T ATE470726T1 AT E470726 T1 ATE470726 T1 AT E470726T1 AT 06827949 T AT06827949 T AT 06827949T AT 06827949 T AT06827949 T AT 06827949T AT E470726 T1 ATE470726 T1 AT E470726T1
- Authority
- AT
- Austria
- Prior art keywords
- cdna
- sample
- polynucleotide
- primer
- obtaining
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/131—Terminal transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
- C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
- C12Q2539/113—Differential Display Analysis [DDA]
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT0192305A AT502823B1 (de) | 2005-11-29 | 2005-11-29 | Polynukleotid-amplifikation |
| PCT/AT2006/000494 WO2007062445A1 (en) | 2005-11-29 | 2006-11-29 | Polynucleotide amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE470726T1 true ATE470726T1 (de) | 2010-06-15 |
Family
ID=37814243
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT0192305A AT502823B1 (de) | 2005-11-29 | 2005-11-29 | Polynukleotid-amplifikation |
| AT06827949T ATE470726T1 (de) | 2005-11-29 | 2006-11-29 | Polynukleotidamplifikation |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT0192305A AT502823B1 (de) | 2005-11-29 | 2005-11-29 | Polynukleotid-amplifikation |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US9394564B2 (de) |
| EP (1) | EP1954829B1 (de) |
| JP (1) | JP5118056B2 (de) |
| KR (1) | KR101327376B1 (de) |
| CN (1) | CN101316937B (de) |
| AT (2) | AT502823B1 (de) |
| CA (1) | CA2630573C (de) |
| DE (1) | DE602006014860D1 (de) |
| WO (1) | WO2007062445A1 (de) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2491131A1 (de) * | 2009-10-21 | 2012-08-29 | Riboxx GmbH | Verfahren zur exponentiellen rna-amplifikation mit wärmestabiler rna-abhängiger rna-polymerase |
| EP2333104A1 (de) | 2009-12-11 | 2011-06-15 | Lexogen GmbH | RNA-Analyseverfahren |
| EP2354243A1 (de) | 2010-02-03 | 2011-08-10 | Lexogen GmbH | Verfahren zur Komplexitätsminderung |
| NZ629913A (en) | 2010-08-23 | 2016-01-29 | Univ Texas | Anti-ox40 antibodies and methods of using the same |
| EP3461914A1 (de) * | 2010-10-22 | 2019-04-03 | Cold Spring Harbor Laboratory | Sortenreine zählung von nukleinsäuren zur gewinnung von informationen über genkopienzahlen |
| EP2492673A1 (de) | 2011-02-28 | 2012-08-29 | Lexogen GmbH | Biosensorarray gebildet aus Knotenpunkten zwischen funktionalisierten Elektroden |
| EP2492674A1 (de) | 2011-02-28 | 2012-08-29 | Lexogen GmbH | Biosensorarray gebildet aus Knotenpunkten zwischen funktionalisierten Elektroden |
| CN103160575B (zh) * | 2011-12-15 | 2014-10-01 | 王荣芳 | SAA1β/β纯合子基因型在肝硬化预后诊断及肝硬化诊断中的应用 |
| LT2828399T (lt) * | 2012-03-22 | 2017-04-10 | Lgc Genomics Limited | Polimerazės grandininės reakcijos aptikimo sistema, panaudojant oligonukleotidus, apimančius fosforotioato grupę |
| EP2684954A1 (de) | 2012-07-10 | 2014-01-15 | Lexogen GmbH | 5'-schutzabhängige Verstärkung |
| WO2014066179A1 (en) * | 2012-10-24 | 2014-05-01 | Clontech Laboratories, Inc. | Template switch-based methods for producing a product nucleic acid |
| KR101922125B1 (ko) | 2012-11-29 | 2018-11-26 | 삼성전자주식회사 | 표적 핵산을 표지하는 방법 |
| EP2921556A1 (de) | 2014-03-21 | 2015-09-23 | Lexogen GmbH | Anzahlerhaltendes RNA-Analyseverfahren |
| CN106978506A (zh) * | 2017-05-05 | 2017-07-25 | 武汉爱基百客生物科技有限公司 | 一种检测大肠杆菌的荧光定量pcr方法 |
| EP3673064A4 (de) | 2017-08-24 | 2021-05-26 | Takara Bio USA, Inc. | Verfahren zur herstellung von nukleinsäuren unter verwendung von durch einen stimulus modifizierten oligonukleotiden |
| CN108949937A (zh) * | 2018-07-16 | 2018-12-07 | 翌圣生物科技（上海）有限公司 | 一步法rt-pcr预混试剂 |
| WO2020037281A1 (en) * | 2018-08-17 | 2020-02-20 | Dana-Farber Cancer Institute, Inc. | Dna amplification method for probe generation |
| PL3899037T3 (pl) * | 2018-12-19 | 2024-04-08 | Illumina, Inc. | Sposoby poprawy priorytetu klonalności klastrów polinukleotydowych |
| WO2020184551A1 (ja) * | 2019-03-13 | 2020-09-17 | 東洋紡株式会社 | 核酸の生成および増幅 |
| US20210381067A1 (en) * | 2020-06-08 | 2021-12-09 | Ampliwise Inc. | Nucleic acid amplification and detection with attenuaiting probe |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990001065A1 (en) * | 1988-07-26 | 1990-02-08 | Genelabs Incorporated | Rna and dna amplification techniques |
| US6582908B2 (en) * | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| ATE231920T1 (de) * | 1992-03-11 | 2003-02-15 | Dana Farber Cancer Inst Inc | Methode um mrna zu klonieren |
| EP0832294A1 (de) * | 1995-06-07 | 1998-04-01 | Pioneer Hi-Bred International, Inc. | Auf pcr bsierendes substraktives cdns klonierungsverfahren |
| US6395887B1 (en) * | 1995-08-01 | 2002-05-28 | Yale University | Analysis of gene expression by display of 3'-end fragments of CDNAS |
| US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| AU2264197A (en) * | 1996-02-09 | 1997-08-28 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Restriction display (rd-pcr) of differentially expressed mrnas |
| US5932451A (en) * | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
| US6287825B1 (en) * | 1998-09-18 | 2001-09-11 | Molecular Staging Inc. | Methods for reducing the complexity of DNA sequences |
| US20020012922A1 (en) | 1998-11-04 | 2002-01-31 | Hilbush Brian S. | Simplified method for indexing and determining the relative concentration of expressed messenger RNAs |
| US6224565B1 (en) * | 1998-11-13 | 2001-05-01 | Sound Surgical Technologies, Llc | Protective sheath and method for ultrasonic probes |
| DE19920611A1 (de) * | 1999-05-05 | 2000-11-09 | Roche Diagnostics Gmbh | Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs |
| EP1235932A2 (de) * | 1999-10-08 | 2002-09-04 | Protogene Laboratories, Inc. | Verfahren und vorrichtung zur durchführung von hohen zahlen von reaktionen unter benutzung von arrays |
| EP1186673A3 (de) * | 2000-09-11 | 2003-03-26 | Agilent Technologies, Inc. (a Delaware corporation) | Kalibrierung von Daten von Molekül-Arrays |
| EP1325118B1 (de) * | 2000-10-05 | 2016-05-25 | Riken | Oligonucleotidlinker mit einem variablen kohesiven anteil und methode zur herstellung von polynucleotidbanken mittels dieser linker |
| US7290274B1 (en) * | 2000-10-20 | 2007-10-30 | Scientific-Atlanta, Inc. | Context sensitive television menu |
| US6955876B2 (en) * | 2000-11-01 | 2005-10-18 | Kane Michael D | Compositions and systems for identifying and comparing expressed genes (mRNAs) in eukaryotic organisms |
| JP2004527236A (ja) * | 2001-02-14 | 2004-09-09 | ベイラー カレッジ オブ メディスン | Ｒｎａ増幅の方法及び組成物 |
| US20030077611A1 (en) | 2001-10-24 | 2003-04-24 | Sention | Methods and systems for dynamic gene expression profiling |
| EP1369480A1 (de) * | 2002-06-05 | 2003-12-10 | Bioneer Corporation | Verfahren zur Herstellung von vollständiger cDNA und darin verwendete Ankersequenz |
| WO2004087952A2 (en) * | 2003-03-31 | 2004-10-14 | Vib Vzw | Genes regulated by hydrogen peroxide stress |
-
2005
- 2005-11-29 AT AT0192305A patent/AT502823B1/de active
-
2006
- 2006-11-29 DE DE602006014860T patent/DE602006014860D1/de active Active
- 2006-11-29 JP JP2008542554A patent/JP5118056B2/ja active Active
- 2006-11-29 US US12/095,485 patent/US9394564B2/en active Active
- 2006-11-29 CN CN200680044723.5A patent/CN101316937B/zh active Active
- 2006-11-29 WO PCT/AT2006/000494 patent/WO2007062445A1/en active Search and Examination
- 2006-11-29 KR KR1020087015868A patent/KR101327376B1/ko active IP Right Grant
- 2006-11-29 AT AT06827949T patent/ATE470726T1/de not_active IP Right Cessation
- 2006-11-29 EP EP06827949A patent/EP1954829B1/de active Active
- 2006-11-29 CA CA2630573A patent/CA2630573C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AT502823B1 (de) | 2007-06-15 |
| US20090311754A1 (en) | 2009-12-17 |
| JP2009517052A (ja) | 2009-04-30 |
| JP5118056B2 (ja) | 2013-01-16 |
| US9394564B2 (en) | 2016-07-19 |
| EP1954829A1 (de) | 2008-08-13 |
| CA2630573A1 (en) | 2007-06-07 |
| WO2007062445A1 (en) | 2007-06-07 |
| EP1954829B1 (de) | 2010-06-09 |
| CN101316937B (zh) | 2016-05-25 |
| CN101316937A (zh) | 2008-12-03 |
| DE602006014860D1 (de) | 2010-07-22 |
| AT502823A4 (de) | 2007-06-15 |
| CA2630573C (en) | 2016-03-15 |
| KR101327376B1 (ko) | 2013-11-08 |
| KR20080080600A (ko) | 2008-09-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE470726T1 (de) | Polynukleotidamplifikation | |
| DE60332873D1 (de) | Rolling-circle rna-amplifikation | |
| CL2004000934A1 (es) | Conjunto de elementos que comprende cebadores para reconocer la linea de maiz tc1507, metodo para detectar la linea de maiz tc1507 o la progenie del mismo en una muestra biologica. | |
| ATE480644T1 (de) | Verfahren zur quantifizierung kleiner rna- moleküle | |
| WO2008070675A3 (en) | Compositions and methods for the detection of small rna | |
| WO2009124255A3 (en) | Methods for transcript analysis | |
| WO2009152928A3 (de) | Verfahren zur quantitativen analyse von nukleinsäuren, marker dafür und deren verwendung | |
| WO2009152353A3 (en) | Reversible nucleosides and nucleotides terminators and their use in dna sequencing | |
| EP2580355A4 (de) | Durch ein oligonukleotid mit veränderter haarnadelstruktur vermittelte reverse transkription und durch base-spacing eingeschränkte quantitative pcr | |
| DE602005020691D1 (de) | Rna-amplifikation auf ligationsbasis | |
| DK1934345T3 (da) | Modifikationer af rna, der fører til en forøget transkriptstabilitet og translationseffektivitet | |
| DK1735459T3 (da) | Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er | |
| ATE483032T1 (de) | Verfahren zur herstellung von oligonukleotiden | |
| WO2010085966A3 (en) | Method for quantification of small rna species | |
| EA201170933A1 (ru) | Анализ нуклеиновых кислот, полученных из единичных клеток | |
| WO2006081222A3 (en) | Isothermal dna amplification | |
| UA99439C2 (ru) | Синтетический промотор, который индуцируется патогенами | |
| WO2010002938A3 (en) | Method for direct amplification from crude nucleic acid samples | |
| WO2006135684A3 (en) | Methods and kits for sense rna synthesis | |
| WO2006050433A3 (en) | Methods for detection of microrna molecules | |
| ATE480549T1 (de) | Neues verfahren für die bisulfitbehandlung | |
| ATE446384T1 (de) | Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates | |
| WO2014052487A8 (en) | Two-primer pcr for microrna multiplex assay | |
| ATE505562T1 (de) | Doppel-oligonukleotid- nukleinsäurenachweisverfahren | |
| MX2023004690A (es) | Transcripcion inversa de polinucleotidos que comprenden nucleotidos no naturales. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |