JP5118056B2 - ポリヌクレオチド増幅 - Google Patents

ポリヌクレオチド増幅 Download PDF

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Publication number
JP5118056B2
JP5118056B2 JP2008542554A JP2008542554A JP5118056B2 JP 5118056 B2 JP5118056 B2 JP 5118056B2 JP 2008542554 A JP2008542554 A JP 2008542554A JP 2008542554 A JP2008542554 A JP 2008542554A JP 5118056 B2 JP5118056 B2 JP 5118056B2
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primer
cdna
rna
tail
primers
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Japanese (ja)
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JP2009517052A5 (enExample
JP2009517052A (ja
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アレクサンダー・ザイツ
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Lexogen GmbH
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Lexogen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/131—Terminal transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
    • C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
    • C12Q2539/113—Differential Display Analysis [DDA]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2008542554A 2005-11-29 2006-11-29 ポリヌクレオチド増幅 Expired - Fee Related JP5118056B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ATA1923/2005 2005-11-29
AT0192305A AT502823B1 (de) 2005-11-29 2005-11-29 Polynukleotid-amplifikation
PCT/AT2006/000494 WO2007062445A1 (en) 2005-11-29 2006-11-29 Polynucleotide amplification

Publications (3)

Publication Number Publication Date
JP2009517052A JP2009517052A (ja) 2009-04-30
JP2009517052A5 JP2009517052A5 (enExample) 2010-01-14
JP5118056B2 true JP5118056B2 (ja) 2013-01-16

Family

ID=37814243

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008542554A Expired - Fee Related JP5118056B2 (ja) 2005-11-29 2006-11-29 ポリヌクレオチド増幅

Country Status (9)

Country Link
US (1) US9394564B2 (enExample)
EP (1) EP1954829B1 (enExample)
JP (1) JP5118056B2 (enExample)
KR (1) KR101327376B1 (enExample)
CN (1) CN101316937B (enExample)
AT (2) AT502823B1 (enExample)
CA (1) CA2630573C (enExample)
DE (1) DE602006014860D1 (enExample)
WO (1) WO2007062445A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2833979C1 (ru) * 2024-02-20 2025-02-03 федеральное государственное автономное образовательное учреждение высшего образования "Пермский национальный исследовательский политехнический университет" Способ управления работой экскаватора

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102597265A (zh) * 2009-10-21 2012-07-18 里博克斯艾克斯有限公司 使用热稳定的rna依赖的rna聚合酶进行指数式扩增rna的方法
EP2333104A1 (en) 2009-12-11 2011-06-15 Lexogen GmbH RNA analytics method
EP2354243A1 (en) 2010-02-03 2011-08-10 Lexogen GmbH Complexity reduction method
LT2609118T (lt) 2010-08-23 2017-04-25 Board Of Regents, The University Of Texas System Anti-ox40 antikūnai ir jų panaudojimo būdai
DK2630263T4 (da) 2010-10-22 2022-02-14 Cold Spring Harbor Laboratory Varital tælling af nucleinsyrer for at opnå information om antal genomiske kopier
EP2492674A1 (en) 2011-02-28 2012-08-29 Lexogen GmbH Biosensor array formed by junctions of functionalized electrodes
EP2492673A1 (en) 2011-02-28 2012-08-29 Lexogen GmbH Biosensor array formed by junctions of functionalized electrodes
CN103160575B (zh) * 2011-12-15 2014-10-01 王荣芳 SAA1β/β纯合子基因型在肝硬化预后诊断及肝硬化诊断中的应用
RS55868B1 (sr) * 2012-03-22 2017-08-31 Lgc Genomics Ltd Sistem za detekciju lančane reakcije polimeraze pomoću oligonukleotida koji sadrže fosforotioatnu grupu
EP2684954A1 (en) 2012-07-10 2014-01-15 Lexogen GmbH 5´ protection dependent amplification
EP2912197B1 (en) * 2012-10-24 2019-08-07 Takara Bio USA, Inc. Template switch-based methods for producing a product nucleic acid
KR101922125B1 (ko) 2012-11-29 2018-11-26 삼성전자주식회사 표적 핵산을 표지하는 방법
EP2921556A1 (en) 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
CN106978506A (zh) * 2017-05-05 2017-07-25 武汉爱基百客生物科技有限公司 一种检测大肠杆菌的荧光定量pcr方法
US11667952B2 (en) 2017-08-24 2023-06-06 Takara Bio Usa, Inc. Methods of producing nucleic acids using oligonucleotides modified by a stimulus
CN108949937A (zh) * 2018-07-16 2018-12-07 翌圣生物科技（上海）有限公司 一步法rt-pcr预混试剂
US20210310061A1 (en) * 2018-08-17 2021-10-07 Dana-Farber Cancer Institute, Inc. Dna amplification method for probe generation
BR112020026667A2 (pt) * 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
WO2020184551A1 (ja) * 2019-03-13 2020-09-17 東洋紡株式会社 核酸の生成および増幅
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe
WO2026082268A1 (en) * 2024-10-14 2026-04-23 Noscendo Gmbh Methods for processing nucleic acid-containing samples

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU632760B2 (en) * 1988-07-26 1993-01-14 Genelabs Technologies, Inc. Rna and dna amplification techniques
US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
EP0592626B1 (en) * 1992-03-11 2003-01-29 Dana-Farber Cancer Institute, Inc. METHODS TO CLONE mRNA
EP0832294A1 (en) * 1995-06-07 1998-04-01 Pioneer Hi-Bred International, Inc. PCR-BASED cDNA SUBTRACTIVE CLONING METHOD
US6395887B1 (en) * 1995-08-01 2002-05-28 Yale University Analysis of gene expression by display of 3'-end fragments of CDNAS
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
AU2264197A (en) * 1996-02-09 1997-08-28 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Restriction display (rd-pcr) of differentially expressed mrnas
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US20020012922A1 (en) 1998-11-04 2002-01-31 Hilbush Brian S. Simplified method for indexing and determining the relative concentration of expressed messenger RNAs
US6224565B1 (en) * 1998-11-13 2001-05-01 Sound Surgical Technologies, Llc Protective sheath and method for ultrasonic probes
DE19920611A1 (de) * 1999-05-05 2000-11-09 Roche Diagnostics Gmbh Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs
AU1075701A (en) * 1999-10-08 2001-04-23 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
EP1186673A3 (en) 2000-09-11 2003-03-26 Agilent Technologies, Inc. (a Delaware corporation) Calibration of molecular array data
JP4340779B2 (ja) * 2000-10-05 2009-10-07 独立行政法人理化学研究所 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法
US7290274B1 (en) * 2000-10-20 2007-10-30 Scientific-Atlanta, Inc. Context sensitive television menu
AU2002218000A1 (en) * 2000-11-01 2002-05-15 Genomic Solutions, Inc. Compositions and systems for identifying and comparing expressed genes (mrnas) in eukaryotic organisms
WO2002065093A2 (en) 2001-02-14 2002-08-22 Baylor College Of Medicine Methods and compositions of amplifying rna
US20030077611A1 (en) * 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
EP1369480A1 (en) * 2002-06-05 2003-12-10 Bioneer Corporation Process for preparation of full-length cDNA and anchor used for the same
WO2004087952A2 (en) * 2003-03-31 2004-10-14 Vib Vzw Genes regulated by hydrogen peroxide stress

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2833979C1 (ru) * 2024-02-20 2025-02-03 федеральное государственное автономное образовательное учреждение высшего образования "Пермский национальный исследовательский политехнический университет" Способ управления работой экскаватора

Also Published As

Publication number Publication date
CN101316937A (zh) 2008-12-03
CN101316937B (zh) 2016-05-25
DE602006014860D1 (de) 2010-07-22
CA2630573C (en) 2016-03-15
US20090311754A1 (en) 2009-12-17
KR20080080600A (ko) 2008-09-04
KR101327376B1 (ko) 2013-11-08
EP1954829A1 (en) 2008-08-13
WO2007062445A1 (en) 2007-06-07
AT502823B1 (de) 2007-06-15
JP2009517052A (ja) 2009-04-30
EP1954829B1 (en) 2010-06-09
US9394564B2 (en) 2016-07-19
CA2630573A1 (en) 2007-06-07
ATE470726T1 (de) 2010-06-15
AT502823A4 (de) 2007-06-15

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