JP2009517052A5 - - Google Patents
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- Publication number
- JP2009517052A5 JP2009517052A5 JP2008542554A JP2008542554A JP2009517052A5 JP 2009517052 A5 JP2009517052 A5 JP 2009517052A5 JP 2008542554 A JP2008542554 A JP 2008542554A JP 2008542554 A JP2008542554 A JP 2008542554A JP 2009517052 A5 JP2009517052 A5 JP 2009517052A5
- Authority
- JP
- Japan
- Prior art keywords
- cdna
- nucleotides
- tail
- primer
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002773 nucleotide Substances 0.000 claims 45
- 125000003729 nucleotide group Chemical group 0.000 claims 45
- 238000000034 method Methods 0.000 claims 41
- 239000002299 complementary DNA Substances 0.000 claims 31
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 21
- 230000003321 amplification Effects 0.000 claims 21
- 238000003199 nucleic acid amplification method Methods 0.000 claims 21
- 108091033319 polynucleotide Proteins 0.000 claims 12
- 239000002157 polynucleotide Substances 0.000 claims 12
- 102000040430 polynucleotide Human genes 0.000 claims 12
- 238000010839 reverse transcription Methods 0.000 claims 12
- 238000011144 upstream manufacturing Methods 0.000 claims 12
- 108020004999 messenger RNA Proteins 0.000 claims 11
- 239000000523 sample Substances 0.000 claims 9
- 230000000295 complement effect Effects 0.000 claims 8
- 102100034343 Integrase Human genes 0.000 claims 6
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 5
- LTFMZDNNPPEQNG-KVQBGUIXSA-N 2'-deoxyguanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@H]1C[C@H](O)[C@@H](COP(O)(O)=O)O1 LTFMZDNNPPEQNG-KVQBGUIXSA-N 0.000 claims 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 4
- 239000000470 constituent Substances 0.000 claims 4
- 239000007787 solid Substances 0.000 claims 4
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims 3
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims 3
- 108091028664 Ribonucleotide Proteins 0.000 claims 3
- 238000006243 chemical reaction Methods 0.000 claims 3
- 239000002336 ribonucleotide Substances 0.000 claims 3
- 125000002652 ribonucleotide group Chemical group 0.000 claims 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims 2
- 108010006785 Taq Polymerase Proteins 0.000 claims 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims 2
- 238000005251 capillar electrophoresis Methods 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 239000011521 glass Substances 0.000 claims 2
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims 2
- 239000003550 marker Substances 0.000 claims 2
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 125000006853 reporter group Chemical group 0.000 claims 2
- 238000006467 substitution reaction Methods 0.000 claims 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims 1
- VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 claims 1
- LOJNBPNACKZWAI-UHFFFAOYSA-N 3-nitro-1h-pyrrole Chemical group [O-][N+](=O)C=1C=CNC=1 LOJNBPNACKZWAI-UHFFFAOYSA-N 0.000 claims 1
- OZFPSOBLQZPIAV-UHFFFAOYSA-N 5-nitro-1h-indole Chemical compound [O-][N+](=O)C1=CC=C2NC=CC2=C1 OZFPSOBLQZPIAV-UHFFFAOYSA-N 0.000 claims 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 claims 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 208000031886 HIV Infections Diseases 0.000 claims 1
- 241000713772 Human immunodeficiency virus 1 Species 0.000 claims 1
- 241000713340 Human immunodeficiency virus 2 Species 0.000 claims 1
- 101710203526 Integrase Proteins 0.000 claims 1
- 241000713869 Moloney murine leukemia virus Species 0.000 claims 1
- OKIZCWYLBDKLSU-UHFFFAOYSA-M N,N,N-Trimethylmethanaminium chloride Chemical compound [Cl-].C[N+](C)(C)C OKIZCWYLBDKLSU-UHFFFAOYSA-M 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- 108010019653 Pwo polymerase Proteins 0.000 claims 1
- 108010065868 RNA polymerase SP6 Proteins 0.000 claims 1
- 239000013614 RNA sample Substances 0.000 claims 1
- 108010001244 Tli polymerase Proteins 0.000 claims 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims 1
- 229960005305 adenosine Drugs 0.000 claims 1
- 238000000246 agarose gel electrophoresis Methods 0.000 claims 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims 1
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 claims 1
- 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 claims 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 claims 1
- 229960003237 betaine Drugs 0.000 claims 1
- 239000002981 blocking agent Substances 0.000 claims 1
- 230000003247 decreasing effect Effects 0.000 claims 1
- VGONTNSXDCQUGY-UHFFFAOYSA-N desoxyinosine Natural products C1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 VGONTNSXDCQUGY-UHFFFAOYSA-N 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 238000001962 electrophoresis Methods 0.000 claims 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 claims 1
- 238000001502 gel electrophoresis Methods 0.000 claims 1
- 229910021645 metal ion Inorganic materials 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- DAZMQARMQFEKQY-UHFFFAOYSA-L oxalate;tetramethylazanium Chemical compound C[N+](C)(C)C.C[N+](C)(C)C.[O-]C(=O)C([O-])=O DAZMQARMQFEKQY-UHFFFAOYSA-L 0.000 claims 1
- 230000001124 posttranscriptional effect Effects 0.000 claims 1
- 230000001915 proofreading effect Effects 0.000 claims 1
- 238000003753 real-time PCR Methods 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
- 229940104230 thymidine Drugs 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
Images
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT0192305A AT502823B1 (de) | 2005-11-29 | 2005-11-29 | Polynukleotid-amplifikation |
| ATA1923/2005 | 2005-11-29 | ||
| PCT/AT2006/000494 WO2007062445A1 (en) | 2005-11-29 | 2006-11-29 | Polynucleotide amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009517052A JP2009517052A (ja) | 2009-04-30 |
| JP2009517052A5 true JP2009517052A5 (enExample) | 2010-01-14 |
| JP5118056B2 JP5118056B2 (ja) | 2013-01-16 |
Family
ID=37814243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008542554A Active JP5118056B2 (ja) | 2005-11-29 | 2006-11-29 | ポリヌクレオチド増幅 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US9394564B2 (enExample) |
| EP (1) | EP1954829B1 (enExample) |
| JP (1) | JP5118056B2 (enExample) |
| KR (1) | KR101327376B1 (enExample) |
| CN (1) | CN101316937B (enExample) |
| AT (2) | AT502823B1 (enExample) |
| CA (1) | CA2630573C (enExample) |
| DE (1) | DE602006014860D1 (enExample) |
| WO (1) | WO2007062445A1 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102597265A (zh) * | 2009-10-21 | 2012-07-18 | 里博克斯艾克斯有限公司 | 使用热稳定的rna依赖的rna聚合酶进行指数式扩增rna的方法 |
| EP2333104A1 (en) | 2009-12-11 | 2011-06-15 | Lexogen GmbH | RNA analytics method |
| EP2354243A1 (en) | 2010-02-03 | 2011-08-10 | Lexogen GmbH | Complexity reduction method |
| PL2609118T3 (pl) | 2010-08-23 | 2017-07-31 | Board Of Regents, The University Of Texas System | Przeciwciała anty-OX40 i sposoby ich stosowania |
| DK2630263T4 (da) | 2010-10-22 | 2022-02-14 | Cold Spring Harbor Laboratory | Varital tælling af nucleinsyrer for at opnå information om antal genomiske kopier |
| EP2492673A1 (en) | 2011-02-28 | 2012-08-29 | Lexogen GmbH | Biosensor array formed by junctions of functionalized electrodes |
| EP2492674A1 (en) | 2011-02-28 | 2012-08-29 | Lexogen GmbH | Biosensor array formed by junctions of functionalized electrodes |
| CN103160575B (zh) * | 2011-12-15 | 2014-10-01 | 王荣芳 | SAA1β/β纯合子基因型在肝硬化预后诊断及肝硬化诊断中的应用 |
| DK2828399T3 (en) * | 2012-03-22 | 2017-04-10 | Lgc Genomics Ltd | SYSTEM FOR DETECTING POLYMERASE CHAIN REACTION USING OLIGONUCLEOTIDS INCLUDING A PHOSPHOROTHIOATE GROUP |
| EP2684954A1 (en) | 2012-07-10 | 2014-01-15 | Lexogen GmbH | 5´ protection dependent amplification |
| WO2014066179A1 (en) * | 2012-10-24 | 2014-05-01 | Clontech Laboratories, Inc. | Template switch-based methods for producing a product nucleic acid |
| KR101922125B1 (ko) | 2012-11-29 | 2018-11-26 | 삼성전자주식회사 | 표적 핵산을 표지하는 방법 |
| EP2921556A1 (en) | 2014-03-21 | 2015-09-23 | Lexogen GmbH | Copy number preserving RNA analysis method |
| CN106978506A (zh) * | 2017-05-05 | 2017-07-25 | 武汉爱基百客生物科技有限公司 | 一种检测大肠杆菌的荧光定量pcr方法 |
| EP3673064A4 (en) * | 2017-08-24 | 2021-05-26 | Takara Bio USA, Inc. | METHOD OF PRODUCING NUCLEIC ACIDS USING STIMULUS MODIFIED OLIGONUCLEOTIDES |
| CN108949937A (zh) * | 2018-07-16 | 2018-12-07 | 翌圣生物科技（上海）有限公司 | 一步法rt-pcr预混试剂 |
| AU2019321683B2 (en) * | 2018-08-17 | 2025-04-03 | Dana-Farber Cancer Institute, Inc. | DNA amplification method for probe generation |
| WO2020132103A1 (en) * | 2018-12-19 | 2020-06-25 | Illumina, Inc. | Methods for improving polynucleotide cluster clonality priority |
| EP3940073A4 (en) * | 2019-03-13 | 2023-03-01 | Toyobo Co., Ltd. | PRODUCTION AND AMPLIFICATION OF NUCLEIC ACIDS |
| US20210381067A1 (en) * | 2020-06-08 | 2021-12-09 | Ampliwise Inc. | Nucleic acid amplification and detection with attenuaiting probe |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU632760B2 (en) * | 1988-07-26 | 1993-01-14 | Genelabs Technologies, Inc. | Rna and dna amplification techniques |
| US6582908B2 (en) * | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| ATE231920T1 (de) * | 1992-03-11 | 2003-02-15 | Dana Farber Cancer Inst Inc | Methode um mrna zu klonieren |
| EP0832294A1 (en) * | 1995-06-07 | 1998-04-01 | Pioneer Hi-Bred International, Inc. | PCR-BASED cDNA SUBTRACTIVE CLONING METHOD |
| US6395887B1 (en) * | 1995-08-01 | 2002-05-28 | Yale University | Analysis of gene expression by display of 3'-end fragments of CDNAS |
| US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| AU2264197A (en) * | 1996-02-09 | 1997-08-28 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Restriction display (rd-pcr) of differentially expressed mrnas |
| US5932451A (en) * | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
| US6287825B1 (en) * | 1998-09-18 | 2001-09-11 | Molecular Staging Inc. | Methods for reducing the complexity of DNA sequences |
| US20020012922A1 (en) | 1998-11-04 | 2002-01-31 | Hilbush Brian S. | Simplified method for indexing and determining the relative concentration of expressed messenger RNAs |
| US6224565B1 (en) * | 1998-11-13 | 2001-05-01 | Sound Surgical Technologies, Llc | Protective sheath and method for ultrasonic probes |
| DE19920611A1 (de) * | 1999-05-05 | 2000-11-09 | Roche Diagnostics Gmbh | Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs |
| EP1235932A2 (en) * | 1999-10-08 | 2002-09-04 | Protogene Laboratories, Inc. | Method and apparatus for performing large numbers of reactions using array assembly |
| EP1186673A3 (en) * | 2000-09-11 | 2003-03-26 | Agilent Technologies, Inc. (a Delaware corporation) | Calibration of molecular array data |
| WO2002028876A2 (en) * | 2000-10-05 | 2002-04-11 | Riken | Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers. |
| US7290274B1 (en) * | 2000-10-20 | 2007-10-30 | Scientific-Atlanta, Inc. | Context sensitive television menu |
| AU2002218000A1 (en) * | 2000-11-01 | 2002-05-15 | Genomic Solutions, Inc. | Compositions and systems for identifying and comparing expressed genes (mrnas) in eukaryotic organisms |
| WO2002065093A2 (en) * | 2001-02-14 | 2002-08-22 | Baylor College Of Medicine | Methods and compositions of amplifying rna |
| US20030077611A1 (en) * | 2001-10-24 | 2003-04-24 | Sention | Methods and systems for dynamic gene expression profiling |
| EP1369480A1 (en) | 2002-06-05 | 2003-12-10 | Bioneer Corporation | Process for preparation of full-length cDNA and anchor used for the same |
| WO2004087952A2 (en) * | 2003-03-31 | 2004-10-14 | Vib Vzw | Genes regulated by hydrogen peroxide stress |
-
2005
- 2005-11-29 AT AT0192305A patent/AT502823B1/de not_active IP Right Cessation
-
2006
- 2006-11-29 CN CN200680044723.5A patent/CN101316937B/zh active Active
- 2006-11-29 WO PCT/AT2006/000494 patent/WO2007062445A1/en not_active Ceased
- 2006-11-29 KR KR1020087015868A patent/KR101327376B1/ko active Active
- 2006-11-29 DE DE602006014860T patent/DE602006014860D1/de active Active
- 2006-11-29 AT AT06827949T patent/ATE470726T1/de not_active IP Right Cessation
- 2006-11-29 US US12/095,485 patent/US9394564B2/en active Active
- 2006-11-29 JP JP2008542554A patent/JP5118056B2/ja active Active
- 2006-11-29 CA CA2630573A patent/CA2630573C/en active Active
- 2006-11-29 EP EP06827949A patent/EP1954829B1/en active Active
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