CN101316937B - 多核苷酸扩增 - Google Patents

多核苷酸扩增 Download PDF

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Publication number
CN101316937B
CN101316937B CN200680044723.5A CN200680044723A CN101316937B CN 101316937 B CN101316937 B CN 101316937B CN 200680044723 A CN200680044723 A CN 200680044723A CN 101316937 B CN101316937 B CN 101316937B
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cdna
tail
rna
primers
primer
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CN101316937A (zh
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A·塞茨
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Lexogen GmbH
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Lexogen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/131—Terminal transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
    • C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
    • C12Q2539/113—Differential Display Analysis [DDA]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN200680044723.5A 2005-11-29 2006-11-29 多核苷酸扩增 Active CN101316937B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AT0192305A AT502823B1 (de) 2005-11-29 2005-11-29 Polynukleotid-amplifikation
ATA1923/2005 2005-11-29
PCT/AT2006/000494 WO2007062445A1 (en) 2005-11-29 2006-11-29 Polynucleotide amplification

Publications (2)

Publication Number Publication Date
CN101316937A CN101316937A (zh) 2008-12-03
CN101316937B true CN101316937B (zh) 2016-05-25

Family

ID=37814243

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200680044723.5A Active CN101316937B (zh) 2005-11-29 2006-11-29 多核苷酸扩增

Country Status (9)

Country Link
US (1) US9394564B2 (enExample)
EP (1) EP1954829B1 (enExample)
JP (1) JP5118056B2 (enExample)
KR (1) KR101327376B1 (enExample)
CN (1) CN101316937B (enExample)
AT (2) AT502823B1 (enExample)
CA (1) CA2630573C (enExample)
DE (1) DE602006014860D1 (enExample)
WO (1) WO2007062445A1 (enExample)

Families Citing this family (20)

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CN102597265A (zh) * 2009-10-21 2012-07-18 里博克斯艾克斯有限公司 使用热稳定的rna依赖的rna聚合酶进行指数式扩增rna的方法
EP2333104A1 (en) 2009-12-11 2011-06-15 Lexogen GmbH RNA analytics method
EP2354243A1 (en) 2010-02-03 2011-08-10 Lexogen GmbH Complexity reduction method
PL2609118T3 (pl) 2010-08-23 2017-07-31 Board Of Regents, The University Of Texas System Przeciwciała anty-OX40 i sposoby ich stosowania
DK2630263T4 (da) 2010-10-22 2022-02-14 Cold Spring Harbor Laboratory Varital tælling af nucleinsyrer for at opnå information om antal genomiske kopier
EP2492673A1 (en) 2011-02-28 2012-08-29 Lexogen GmbH Biosensor array formed by junctions of functionalized electrodes
EP2492674A1 (en) 2011-02-28 2012-08-29 Lexogen GmbH Biosensor array formed by junctions of functionalized electrodes
CN103160575B (zh) * 2011-12-15 2014-10-01 王荣芳 SAA1β/β纯合子基因型在肝硬化预后诊断及肝硬化诊断中的应用
DK2828399T3 (en) * 2012-03-22 2017-04-10 Lgc Genomics Ltd SYSTEM FOR DETECTING POLYMERASE CHAIN REACTION USING OLIGONUCLEOTIDS INCLUDING A PHOSPHOROTHIOATE GROUP
EP2684954A1 (en) 2012-07-10 2014-01-15 Lexogen GmbH 5´ protection dependent amplification
WO2014066179A1 (en) * 2012-10-24 2014-05-01 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
KR101922125B1 (ko) 2012-11-29 2018-11-26 삼성전자주식회사 표적 핵산을 표지하는 방법
EP2921556A1 (en) 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
CN106978506A (zh) * 2017-05-05 2017-07-25 武汉爱基百客生物科技有限公司 一种检测大肠杆菌的荧光定量pcr方法
EP3673064A4 (en) * 2017-08-24 2021-05-26 Takara Bio USA, Inc. METHOD OF PRODUCING NUCLEIC ACIDS USING STIMULUS MODIFIED OLIGONUCLEOTIDES
CN108949937A (zh) * 2018-07-16 2018-12-07 翌圣生物科技（上海）有限公司 一步法rt-pcr预混试剂
AU2019321683B2 (en) * 2018-08-17 2025-04-03 Dana-Farber Cancer Institute, Inc. DNA amplification method for probe generation
WO2020132103A1 (en) * 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority
EP3940073A4 (en) * 2019-03-13 2023-03-01 Toyobo Co., Ltd. PRODUCTION AND AMPLIFICATION OF NUCLEIC ACIDS
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe

Citations (4)

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US5962272A (en) * 1996-01-03 1999-10-05 Clontech Laboratories, Inc. Methods and compositions for full-length cDNA Cloning using a template-switching oligonucleotide
US6114149A (en) * 1988-07-26 2000-09-05 Genelabs Technologies, Inc. Amplification of mixed sequence nucleic acid fragments
WO2002065093A3 (en) * 2001-02-14 2002-10-10 Baylor College Medicine Methods and compositions of amplifying rna
WO2003035841A2 (en) * 2001-10-24 2003-05-01 Sention, Inc Methods and systems for dynamic gene expression profiling

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ATE231920T1 (de) * 1992-03-11 2003-02-15 Dana Farber Cancer Inst Inc Methode um mrna zu klonieren
EP0832294A1 (en) * 1995-06-07 1998-04-01 Pioneer Hi-Bred International, Inc. PCR-BASED cDNA SUBTRACTIVE CLONING METHOD
US6395887B1 (en) * 1995-08-01 2002-05-28 Yale University Analysis of gene expression by display of 3'-end fragments of CDNAS
AU2264197A (en) * 1996-02-09 1997-08-28 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Restriction display (rd-pcr) of differentially expressed mrnas
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US20020012922A1 (en) 1998-11-04 2002-01-31 Hilbush Brian S. Simplified method for indexing and determining the relative concentration of expressed messenger RNAs
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EP1235932A2 (en) * 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
EP1186673A3 (en) * 2000-09-11 2003-03-26 Agilent Technologies, Inc. (a Delaware corporation) Calibration of molecular array data
WO2002028876A2 (en) * 2000-10-05 2002-04-11 Riken Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers.
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US6114149A (en) * 1988-07-26 2000-09-05 Genelabs Technologies, Inc. Amplification of mixed sequence nucleic acid fragments
US5962272A (en) * 1996-01-03 1999-10-05 Clontech Laboratories, Inc. Methods and compositions for full-length cDNA Cloning using a template-switching oligonucleotide
WO2002065093A3 (en) * 2001-02-14 2002-10-10 Baylor College Medicine Methods and compositions of amplifying rna
WO2003035841A2 (en) * 2001-10-24 2003-05-01 Sention, Inc Methods and systems for dynamic gene expression profiling

Also Published As

Publication number Publication date
CA2630573C (en) 2016-03-15
JP5118056B2 (ja) 2013-01-16
US20090311754A1 (en) 2009-12-17
KR101327376B1 (ko) 2013-11-08
DE602006014860D1 (de) 2010-07-22
US9394564B2 (en) 2016-07-19
AT502823B1 (de) 2007-06-15
CN101316937A (zh) 2008-12-03
WO2007062445A1 (en) 2007-06-07
JP2009517052A (ja) 2009-04-30
ATE470726T1 (de) 2010-06-15
EP1954829A1 (en) 2008-08-13
EP1954829B1 (en) 2010-06-09
AT502823A4 (de) 2007-06-15
KR20080080600A (ko) 2008-09-04
CA2630573A1 (en) 2007-06-07

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